Rh2AG618900

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008673
Physical Location & Seq
Reverse (-)
84272098 .. 84272871
774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG618900.1

Sequence Viewer

Length: 774 bp
ATGGGATTCTTCACCTTCGCAGTCGCAGGCGGCGGCCTGATTCTGATGGGCACATTCTCCTCCACCGTCGACCCATCTCCACCGTCCACTCATCCCCAATCGAAACCGCAAACGACCCAGTACAAATCGTCGTTCATCTACTTCCTCCTCGCATCCATTCTCTCCTTCCTCTTCATTCTCAACTCGTTGGTTTCTTTCTTCGACGCCTTAAACTCAAACGATCGGGTCGGGTCGGCCCAGCAGCTCCAGGTCCTCGCGATTGCCGCCCTCTTCTTCCTCTACTCCATCACCGGCCTCCTGGTAAACTTCACCAACTCATCTTTCCCTTCTTCGCTGCTCAATTTGATCGTCCTCTTCGGTTTCGTTGAAGAATTCATGTACTTTTATCTTCAAAGAAAAGACACTGCTGGAATCGAGAATCGATACTTTGATCTGCTGCTGGTGCCGATTTTCGTCTGTGTCTTCTCCACAGTTCTTGAATTGCACAACCCTAAATCGGTTTTCCCCAAATTGGCTCGCGGAGTTGGCTTGGTTCTACAAGGACTGTGGTTCTTGCAAATGGGTCTTTCGATTTATACGCATTGGATGGCTCATGGGTGCTCATTGAAGGAAAAGAGTAGAGGGAATTACACGGTTAAGTGTAAGAGGCACCCTGAATATCACAGGGCTAGAGCTATTGCTACGCTTCAGTTTAACTGCCATTTGGCTCTGATAGTGGTTTTGGTGGTGGGAGTGTATTCGATTGTGGCGAGCGGAAATGCAGCATCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

257

Amino Acids

28.51

Weight (kDa)

9.05

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 111 - 246 3.2e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 442, 648
AccBSI CCGCTC 1 cut(s) 753
AccI GTMKAC 1 cut(s) 69
AccII CGCG 2 cut(s) 257, 519
AciI CCGC 6 cut(s) 30, 33, 107, 264, 519, 753
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 122, 380
AfiI CCNNNNNNNGG 2 cut(s) 496, 511
AgsI TTSAA 4 cut(s) 368, 392, 479, 607
AjnI CCWGG 2 cut(s) 246, 297
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 2 cut(s) 244, 674
AluI AGCT 2 cut(s) 244, 674
Alw21I GWGCWC 1 cut(s) 602
AoxI GGCC 3 cut(s) 34, 234, 292
ApeKI GCWGC 4 cut(s) 241, 334, 436, 761
ApoI RAATTY 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 235, 250
AsuHPI GGTGA 3 cut(s) 4, 280, 301
AvaII GGWCC 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 53
BanI GGYRCC 2 cut(s) 442, 648
BbsI GAAGAC 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 602
BbvI GCAGC 3 cut(s) 253, 321, 423
BccI CCATC 4 cut(s) 40, 82, 293, 580
BciT130I CCWGG 2 cut(s) 248, 299
BfaI CTAG 1 cut(s) 669
BisI GCNGC 7 cut(s) 31, 34, 242, 264, 335, 437, 762
BlsI GCNGC 7 cut(s) 32, 35, 243, 265, 336, 438, 763
Bme1390I CCNGG 2 cut(s) 248, 299
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 235, 250
BmiI GGNNCC 2 cut(s) 444, 650
BmrFI CCNGG 2 cut(s) 248, 299
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 161
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 421
BsaHI GRCGYC 1 cut(s) 204
Bsc4I CCNNNNNNNGG 2 cut(s) 496, 511
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 118
BseBI CCWGG 2 cut(s) 248, 299
BseCI ATCGAT 1 cut(s) 421
BseGI GGATG 3 cut(s) 91, 152, 591
BseLI CCNNNNNNNGG 2 cut(s) 496, 511
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 2 cut(s) 49, 137
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 3 cut(s) 253, 321, 423
BseYI CCCAGC 1 cut(s) 237
Bsh1236I CGCG 2 cut(s) 257, 519
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 36, 236, 294
BshNI GGYRCC 2 cut(s) 442, 648
BshVI ATCGAT 1 cut(s) 421
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 602
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 496, 511
BsnI GGCC 3 cut(s) 36, 236, 294
Bsp1286I GDGCHC 2 cut(s) 53, 602
Bsp143I GATC 3 cut(s) 220, 345, 430
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 6 cut(s) 30, 33, 107, 264, 519, 753
BspANI GGCC 3 cut(s) 36, 236, 294
BspDI ATCGAT 1 cut(s) 421
BspFNI CGCG 2 cut(s) 257, 519
BspLI GGNNCC 2 cut(s) 444, 650
BspT107I GGYRCC 2 cut(s) 442, 648
BsrBI CCGCTC 1 cut(s) 753
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 290
BssMI GATC 3 cut(s) 220, 345, 430
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 248, 299
Bst4CI ACNGT 5 cut(s) 67, 84, 472, 546, 634
Bst6I CTCTTC 3 cut(s) 176, 275, 359
BstACI GRCGYC 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 28, 517, 751
BstF5I GGATG 3 cut(s) 91, 152, 591
BstFNI CGCG 2 cut(s) 257, 519
BstKTI GATC 3 cut(s) 223, 348, 433
BstMBI GATC 3 cut(s) 220, 345, 430
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 3 cut(s) 263, 442, 525
BstNI CCWGG 2 cut(s) 248, 299
BstSCI CCNGG 2 cut(s) 246, 297
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 2 cut(s) 257, 519
BstV1I GCAGC 3 cut(s) 253, 321, 423
BstV2I GAAGAC 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 421
BsuRI GGCC 3 cut(s) 36, 236, 294
BsuTUI ATCGAT 1 cut(s) 421
BtsCI GGATG 3 cut(s) 91, 152, 591
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
BtuMI TCGCGA 1 cut(s) 257
Cac8I GCNNGC 3 cut(s) 28, 517, 751
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 235, 250
ClaI ATCGAT 1 cut(s) 421
CseI GACGC 1 cut(s) 212
Csp6I GTAC 2 cut(s) 121, 379
CspCI CAANNNNNGTGG 4 cut(s) 457, 492, 527, 562
CviAII CATG 2 cut(s) 376, 593
CviQI GTAC 2 cut(s) 121, 379
DpnI GATC 3 cut(s) 222, 347, 432
DpnII GATC 3 cut(s) 220, 345, 430
Eam1104I CTCTTC 3 cut(s) 176, 275, 359
EarI CTCTTC 3 cut(s) 176, 275, 359
Eco47I GGWCC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 246, 297
FaeI CATG 2 cut(s) 379, 596
FaiI YATR 3 cut(s) 377, 576, 594
FatI CATG 2 cut(s) 375, 592
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 7 cut(s) 31, 34, 242, 264, 335, 437, 762
FokI GGATG 3 cut(s) 78, 139, 598
Fsp4HI GCNGC 7 cut(s) 31, 34, 242, 264, 335, 437, 762
FspBI CTAG 1 cut(s) 669
GluI GCNGC 7 cut(s) 31, 34, 242, 264, 335, 437, 762
GsaI CCCAGC 1 cut(s) 241
GsuI CTGGAG 1 cut(s) 230
HaeIII GGCC 3 cut(s) 36, 236, 294
HapII CCGG 1 cut(s) 291
HgaI GACGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 379, 596
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 4 cut(s) 6, 40, 411, 418
HpaII CCGG 1 cut(s) 291
HphI GGTGA 3 cut(s) 4, 280, 301
Hpy166II GTNNAC 3 cut(s) 70, 87, 304
Hpy188I TCNGA 3 cut(s) 45, 711, 769
Hpy188III TCNNGA 3 cut(s) 256, 415, 476
Hpy8I GTNNAC 3 cut(s) 70, 87, 304
Hpy99I CGWCG 3 cut(s) 71, 133, 206
HpyAV CCTTC 4 cut(s) 25, 175, 336, 601
HpyCH4III ACNGT 5 cut(s) 67, 84, 472, 546, 634
HpyCH4V TGCA 3 cut(s) 484, 556, 761
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 442, 525
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 379, 596
Kzo9I GATC 3 cut(s) 220, 345, 430
LmnI GCTCC 1 cut(s) 249
Lsp1109I GCAGC 3 cut(s) 253, 321, 423
LweI GCATC 1 cut(s) 161
MaeI CTAG 1 cut(s) 669
MalI GATC 3 cut(s) 222, 347, 432
MbiI CCGCTC 1 cut(s) 753
MboI GATC 3 cut(s) 220, 345, 430
MboII GAAGA 9 cut(s) 163, 190, 262, 265, 321, 346, 380, 380, 454
MhlI GDGCHC 2 cut(s) 53, 602
MluCI AATT 5 cut(s) 340, 371, 479, 509, 625
MseI TTAA 4 cut(s) 209, 636, 693, 772
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 2 cut(s) 248, 299
MvaI CCWGG 2 cut(s) 248, 299
MvnI CGCG 2 cut(s) 257, 519
MwoI GCNNNNNNNGC 3 cut(s) 263, 442, 525
NdeII GATC 3 cut(s) 220, 345, 430
NlaIII CATG 2 cut(s) 379, 596
NlaIV GGNNCC 2 cut(s) 444, 650
NruI TCGCGA 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 746
PfeI GAWTC 4 cut(s) 6, 40, 411, 418
PkrI GCNGC 7 cut(s) 32, 35, 243, 265, 336, 438, 763
Ple19I CGATCG 1 cut(s) 223
PpuMI RGGWCCY 1 cut(s) 250
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 246, 297
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 2 cut(s) 246, 297
PspN4I GGNNCC 2 cut(s) 444, 650
PspPI GGNCC 2 cut(s) 235, 250
PspPPI RGGWCCY 1 cut(s) 250
PvuI CGATCG 1 cut(s) 223
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 2 cut(s) 122, 380
RsaNI GTAC 2 cut(s) 121, 379
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 4 cut(s) 209, 636, 693, 772
SatI GCNGC 7 cut(s) 31, 34, 242, 264, 335, 437, 762
Sau3AI GATC 3 cut(s) 220, 345, 430
Sau96I GGNCC 2 cut(s) 235, 250
ScrFI CCNGG 2 cut(s) 248, 299
SduI GDGCHC 2 cut(s) 53, 602
SetI ASST 4 cut(s) 17, 246, 252, 676
SfaNI GCATC 1 cut(s) 161
SinI GGWCC 1 cut(s) 250
Sse9I AATT 5 cut(s) 340, 371, 479, 509, 625
SsiI CCGC 6 cut(s) 30, 33, 107, 264, 519, 753
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 246, 297
TaaI ACNGT 5 cut(s) 67, 84, 472, 546, 634
TaqI TCGA 7 cut(s) 69, 101, 201, 414, 421, 569, 740
TasI AATT 5 cut(s) 340, 371, 479, 509, 625
TatI WGTACW 2 cut(s) 120, 378
TauI GCSGC 3 cut(s) 33, 36, 266
TfiI GAWTC 4 cut(s) 6, 40, 411, 418
Tru1I TTAA 4 cut(s) 209, 636, 693, 772
Tru9I TTAA 4 cut(s) 209, 636, 693, 772
TscAI CASTG 1 cut(s) 409
TseI GCWGC 4 cut(s) 241, 334, 436, 761
TspDTI ATGAA 3 cut(s) 124, 163, 364
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.