MD17G1040800.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008673
Physical Location & Seq
Forward (+)
2966451 .. 2967443
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1040800.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGGTTTTCTCACATTCGCCGCCGCAGGCGGCGGCCTGATCTTGATGGGCGCGTACGAAGCCATTTCCTCCTCAATCCAAAACCCAGATTCAGATTCATCTCCGCCGTCGTCGCCTCAACCTATGATTCGTCCCCCGATGGCGCAGTCGCAAGCGAAAACCCAGTCTCGGTTGTCGTCGATTTATTACCTGGCAGCCGCCGTTGTTTCGTTGCTGTTCATCTTGAACTCTTTGGTTTCGTTCTTCGACGCGCTCGACTCAGCGGACCCAGTCGGCTCGGCTATGCAATTGCAAGTGCTGGCGATCGCGTCGCTGTTCTTGCTCTACGCGATTACGGGTCTTGTGGTAAATTTCACGAATTCAACTCTACCTTGTTCGTTGCTTAGTTTGGTCGGTCTTTTCGCTTTCGTTGAAGAATTTTTAATGTTTTACATGCAAAGAAAGGACACTTTCGGAATCGAAAATCGGTACTTTGATCTGCTGCTTGTGCCAATTGCTGTGTGTATATTTTCTACAATGCTTGAATTCAACAACCCCAAATCGAATTATCCGAAATTGGCTCGTGGGGTTGGCTTGGTTCTGCAAGGAATGTGGTTTCTGCAAATGGGCATTTCTTTGTACACCAACTTCATGGCGCACGGATGCTCGTTGCACGAAAAGAGTAGAGGGAATTACACTGTGAAGTGCAAGGGGCACCCTGAGTATCACAGGGCTAGAGCCATCGTCACGCTTCAGTTTAACTGCCATCTTGCTTTTGTGGTGGTTGTGGTTGTGGGAGTGTACTCGATTATTGCCAGGAAGCGTGGCGGATTTCGGGGTGATGTTCCGCACTATAAGCCGCTTGGCGCAGAGATACATCAGTTTGATAATCAGAGCCAATTTACTTTGGATTCTGATGATGGCGATGATGAGGTTAAAGAGGAGGGGAATTCGGCAATGCAGAAAGCCGGCGAAGTGGAATTGGGTGTCAACGGCTATGGTTCTCATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

331

Amino Acids

36.12

Weight (kDa)

6.06

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 126 - 261 1.2e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 693
AccII CGCG 4 cut(s) 53, 251, 308, 329
AcsI RAATTY 5 cut(s) 349, 358, 416, 524, 928
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 4 cut(s) 56, 470, 620, 782
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 5 cut(s) 226, 363, 413, 524, 529
AjnI CCWGG 2 cut(s) 189, 794
Alw26I GTCTC 1 cut(s) 171
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 194, 481
ApoI RAATTY 5 cut(s) 349, 358, 416, 524, 928
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AsiSI GCGATCGC 1 cut(s) 306
AspLEI GCGC 5 cut(s) 53, 145, 253, 637, 848
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 1 cut(s) 830
AvaII GGWCC 1 cut(s) 265
BaeGI GKGCMC 1 cut(s) 696
BaeI ACNNNNGTAYC 2 cut(s) 845, 878
BanI GGYRCC 1 cut(s) 693
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BauI CACGAG 1 cut(s) 561
BbvI GCAGC 2 cut(s) 206, 468
BccI CCATC 5 cut(s) 40, 133, 728, 753, 893
BceAI ACGGC 3 cut(s) 91, 185, 988
BciT130I CCWGG 2 cut(s) 191, 796
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 714
BisI GCNGC 8 cut(s) 21, 24, 31, 34, 195, 198, 482, 839
BlsI GCNGC 8 cut(s) 22, 25, 32, 35, 196, 199, 483, 840
Bme1390I CCNGG 2 cut(s) 191, 796
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 2 cut(s) 267, 695
BmrFI CCNGG 2 cut(s) 191, 796
BmrI ACTGGG 2 cut(s) 157, 263
BmsI GCATC 1 cut(s) 632
BmuI ACTGGG 2 cut(s) 157, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse118I RCCGGY 1 cut(s) 947
Bse1I ACTGG 2 cut(s) 163, 269
Bse3DI GCAATG 1 cut(s) 942
BseBI CCWGG 2 cut(s) 191, 796
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 942
BseMII CTCAG 2 cut(s) 273, 690
BseNI ACTGG 2 cut(s) 163, 269
BseRI GAGGAG 2 cut(s) 61, 935
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 2 cut(s) 206, 468
Bsh1236I CGCG 4 cut(s) 53, 251, 308, 329
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 693
BsiEI CGRYCG 1 cut(s) 306
BsiSI CCGG 1 cut(s) 948
BsiWI CGTACG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 3 cut(s) 39, 303, 475
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 2 cut(s) 272, 691
BspFNI CGCG 4 cut(s) 53, 251, 308, 329
BspLI GGNNCC 2 cut(s) 267, 695
BspT107I GGYRCC 1 cut(s) 693
BsrDI GCAATG 1 cut(s) 942
BsrFI RCCGGY 1 cut(s) 947
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 2 cut(s) 163, 269
BssAI RCCGGY 1 cut(s) 947
BssMI GATC 3 cut(s) 39, 303, 475
BssSI CACGAG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 191, 796
Bst4CI ACNGT 1 cut(s) 679
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 4 cut(s) 28, 153, 300, 949
BstDEI CTNAG 3 cut(s) 259, 383, 699
BstF5I GGATG 1 cut(s) 647
BstFNI CGCG 4 cut(s) 53, 251, 308, 329
BstHHI GCGC 5 cut(s) 53, 145, 253, 637, 848
BstKTI GATC 3 cut(s) 42, 306, 478
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 3 cut(s) 39, 303, 475
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 5 cut(s) 59, 112, 319, 487, 835
BstNI CCWGG 2 cut(s) 191, 796
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 189, 794
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 4 cut(s) 53, 251, 308, 329
BstV1I GCAGC 2 cut(s) 206, 468
BstXI CCANNNNNNTGG 1 cut(s) 631
BsuRI GGCC 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 675
Cac8I GCNNGC 4 cut(s) 28, 153, 300, 949
CfoI GCGC 5 cut(s) 53, 145, 253, 637, 848
Cfr10I RCCGGY 1 cut(s) 947
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 2 cut(s) 257, 297
Csp6I GTAC 4 cut(s) 55, 469, 619, 781
CspCI CAANNNNNGTGG 2 cut(s) 572, 607
CviAII CATG 2 cut(s) 433, 631
CviQI GTAC 4 cut(s) 55, 469, 619, 781
DdeI CTNAG 3 cut(s) 259, 383, 699
DpnI GATC 3 cut(s) 41, 305, 477
DpnII GATC 3 cut(s) 39, 303, 475
EciI GGCGGA 2 cut(s) 93, 822
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 716
EcoRI GAATTC 3 cut(s) 358, 524, 928
EcoRII CCWGG 2 cut(s) 189, 794
FaeI CATG 2 cut(s) 436, 634
FaiI YATR 8 cut(s) 125, 284, 434, 506, 632, 834, 978, 987
FaqI GGGAC 1 cut(s) 117
FatI CATG 2 cut(s) 432, 630
Fnu4HI GCNGC 8 cut(s) 21, 24, 31, 34, 195, 198, 482, 839
FokI GGATG 1 cut(s) 654
Fsp4HI GCNGC 8 cut(s) 21, 24, 31, 34, 195, 198, 482, 839
FspBI CTAG 1 cut(s) 714
GlaI GCGC 5 cut(s) 52, 144, 252, 636, 847
GluI GCNGC 8 cut(s) 21, 24, 31, 34, 195, 198, 482, 839
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 948
HgaI GACGC 2 cut(s) 257, 297
HhaI GCGC 5 cut(s) 53, 145, 253, 637, 848
Hin1II CATG 2 cut(s) 436, 634
Hin6I GCGC 5 cut(s) 51, 143, 251, 635, 846
HinP1I GCGC 5 cut(s) 51, 143, 251, 635, 846
HincII GTYRAC 1 cut(s) 970
HindII GTYRAC 1 cut(s) 970
HinfI GANTC 6 cut(s) 89, 95, 127, 257, 456, 890
HpaII CCGG 1 cut(s) 948
HphI GGTGA 1 cut(s) 830
Hpy166II GTNNAC 3 cut(s) 621, 781, 970
Hpy188I TCNGA 5 cut(s) 94, 455, 552, 873, 895
Hpy188III TCNNGA 3 cut(s) 43, 223, 355
Hpy8I GTNNAC 3 cut(s) 621, 781, 970
Hpy99I CGWCG 5 cut(s) 112, 115, 181, 251, 313
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4V TGCA 8 cut(s) 286, 292, 436, 583, 601, 652, 687, 940
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 112, 319, 487, 835
HpyF3I CTNAG 3 cut(s) 259, 383, 699
Hsp92II CATG 2 cut(s) 436, 634
HspAI GCGC 5 cut(s) 51, 143, 251, 635, 846
KroI GCCGGC 1 cut(s) 947
KroNI GCCGGC 1 cut(s) 949
Kzo9I GATC 3 cut(s) 39, 303, 475
Lsp1109I GCAGC 2 cut(s) 206, 468
LweI GCATC 1 cut(s) 632
MaeI CTAG 1 cut(s) 714
MaeIII GTNAC 1 cut(s) 724
MalI GATC 3 cut(s) 41, 305, 477
MboI GATC 3 cut(s) 39, 303, 475
MboII GAAGA 2 cut(s) 235, 425
MfeI CAATTG 2 cut(s) 287, 492
MhlI GDGCHC 1 cut(s) 696
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 7 cut(s) 79, 82, 126, 659, 904, 913, 916
MroNI GCCGGC 1 cut(s) 947
MseI TTAA 3 cut(s) 422, 738, 915
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 948
MspR9I CCNGG 2 cut(s) 191, 796
MunI CAATTG 2 cut(s) 287, 492
MvaI CCWGG 2 cut(s) 191, 796
MvnI CGCG 4 cut(s) 53, 251, 308, 329
MwoI GCNNNNNNNGC 5 cut(s) 59, 112, 319, 487, 835
NaeI GCCGGC 1 cut(s) 949
NdeII GATC 3 cut(s) 39, 303, 475
NgoMIV GCCGGC 1 cut(s) 947
NlaIII CATG 2 cut(s) 436, 634
NlaIV GGNNCC 2 cut(s) 267, 695
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 1 cut(s) 724
NspI RCATGY 1 cut(s) 436
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 548
PdiI GCCGGC 1 cut(s) 949
PfeI GAWTC 5 cut(s) 89, 95, 127, 456, 890
Pfl23II CGTACG 1 cut(s) 54
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 8 cut(s) 22, 25, 32, 35, 196, 199, 483, 840
Ple19I CGATCG 1 cut(s) 306
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 2 cut(s) 189, 794
PspGI CCWGG 2 cut(s) 189, 794
PspLI CGTACG 1 cut(s) 54
PspN4I GGNNCC 2 cut(s) 267, 695
PspPI GGNCC 1 cut(s) 265
PsyI GACNNNGTC 1 cut(s) 269
PvuI CGATCG 1 cut(s) 306
RgaI GCGATCGC 1 cut(s) 306
RsaI GTAC 4 cut(s) 56, 470, 620, 782
RsaNI GTAC 4 cut(s) 55, 469, 619, 781
SaqAI TTAA 3 cut(s) 422, 738, 915
SatI GCNGC 8 cut(s) 21, 24, 31, 34, 195, 198, 482, 839
Sau3AI GATC 3 cut(s) 39, 303, 475
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 2 cut(s) 191, 796
SduI GDGCHC 1 cut(s) 696
SetI ASST 4 cut(s) 124, 192, 373, 915
SfaAI GCGATCGC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 632
SgfI GCGATCGC 1 cut(s) 306
SinI GGWCC 1 cut(s) 265
SspMI CTAG 1 cut(s) 714
StyD4I CCNGG 2 cut(s) 189, 794
TaaI ACNGT 1 cut(s) 679
TaqI TCGA 6 cut(s) 179, 246, 255, 459, 542, 785
TaqII GACCGA 1 cut(s) 383
TatI WGTACW 2 cut(s) 618, 780
TauI GCSGC 6 cut(s) 23, 26, 33, 36, 200, 841
TfiI GAWTC 5 cut(s) 89, 95, 127, 456, 890
Tru1I TTAA 3 cut(s) 422, 738, 915
Tru9I TTAA 3 cut(s) 422, 738, 915
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 2 cut(s) 194, 481
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 3 cut(s) 87, 208, 619
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 1 cut(s) 682
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 5 cut(s) 349, 358, 416, 524, 928
XceI RCATGY 1 cut(s) 436
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.