Rh2DG643000

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008673
Physical Location & Seq
Reverse (-)
87035733 .. 87037376
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG643000.1

Sequence Viewer

Length: 570 bp
ATGTACTTTTATCTTCAAAGAAAAGACACTGCTGGAATCGAGAATCGATACTTTGATCTGCTGCTGGTGCCGATTTTCGTCTGTGTCTTCTCCACAGTTCTTGAATTGCACAACCCTAAATCGGTTTTCCCCAAATTGGCTCGCGGAGTTGGCTTGGTTCTACAAGGACTGTGGTTCCTGCAAATGGGTCTTTCCATTTATACGCATTGGATGGCTCATGGGTGCTCATTGAAGGAAAAGAGTAGAGGGAATTACACGGTTAAGTGTAAGGGGCACCCTGAATATCATAGGGCTAGAGCTATCGCTACGCTTCAGTTTAACTGCCATTTGGCTCTGATAGTGGTTTTGGTGGTGGGGCTGTATTCGATTGTGGCGAGGAAAACAGGAGTTGTTGCTGATTTCTCGAGCTATAAGCCTCTTGGCGCGGAGATGCAGCATCAGATTGATAGTCAGGGGCAGTTTACTTTGGATTCGGAGGATGAGGAGGTTAGAGAAGAGGGCAATGTGGTTATGAACAAGGCCGGCGGGGAAGTGGAGTTGGGTGTAAATGGTAATGGGACTTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

189

Amino Acids

21.17

Weight (kDa)

7.71

Isoelectric Point (pI)

29.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 2 - 121 1.3e-41 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 564
AccB1I GGYRCC 2 cut(s) 67, 273
AccII CGCG 2 cut(s) 144, 425
AciI CCGC 3 cut(s) 144, 425, 525
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 3 cut(s) 121, 136, 184
AgsI TTSAA 3 cut(s) 17, 104, 232
AluBI AGCT 2 cut(s) 299, 408
AluI AGCT 2 cut(s) 299, 408
Alw21I GWGCWC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 403
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 2 cut(s) 61, 433
AspLEI GCGC 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 403
BaeGI GKGCMC 1 cut(s) 276
BanI GGYRCC 2 cut(s) 67, 273
BbsI GAAGAC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 48, 445
BccI CCATC 1 cut(s) 205
BfaI CTAG 1 cut(s) 294
BisI GCNGC 2 cut(s) 62, 434
BlsI GCNGC 2 cut(s) 63, 435
BmeT110I CYCGRG 1 cut(s) 403
BmiI GGNNCC 3 cut(s) 69, 176, 275
BmsI GCATC 2 cut(s) 420, 445
BpiI GAAGAC 1 cut(s) 79
Bsa29I ATCGAT 1 cut(s) 46
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 136, 184
Bse118I RCCGGY 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 508
BseCI ATCGAT 1 cut(s) 46
BseGI GGATG 2 cut(s) 216, 484
BseLI CCNNNNNNNGG 3 cut(s) 121, 136, 184
BseMI GCAATG 1 cut(s) 508
BseRI GAGGAG 1 cut(s) 497
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 48, 445
Bsh1236I CGCG 2 cut(s) 144, 425
BshFI GGCC 1 cut(s) 521
BshNI GGYRCC 2 cut(s) 67, 273
BshVI ATCGAT 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 227
BsiHKCI CYCGRG 1 cut(s) 403
BsiSI CCGG 1 cut(s) 522
BslI CCNNNNNNNGG 3 cut(s) 121, 136, 184
BsnI GGCC 1 cut(s) 521
BsoBI CYCGRG 1 cut(s) 403
Bsp1286I GDGCHC 2 cut(s) 227, 276
Bsp143I GATC 1 cut(s) 55
BspACI CCGC 3 cut(s) 144, 425, 525
BspANI GGCC 1 cut(s) 521
BspDI ATCGAT 1 cut(s) 46
BspFNI CGCG 2 cut(s) 144, 425
BspLI GGNNCC 3 cut(s) 69, 176, 275
BspT107I GGYRCC 2 cut(s) 67, 273
BsrDI GCAATG 1 cut(s) 508
BsrFI RCCGGY 1 cut(s) 521
BssAI RCCGGY 1 cut(s) 521
BssMI GATC 1 cut(s) 55
Bst4CI ACNGT 3 cut(s) 97, 171, 259
Bst6I CTCTTC 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 142, 523
BstF5I GGATG 2 cut(s) 216, 484
BstFNI CGCG 2 cut(s) 144, 425
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 1 cut(s) 58
BstMBI GATC 1 cut(s) 55
BstMWI GCNNNNNNNGC 2 cut(s) 67, 150
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 2 cut(s) 144, 425
BstV1I GCAGC 2 cut(s) 48, 445
BstV2I GAAGAC 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 46
BsuRI GGCC 1 cut(s) 521
BsuTUI ATCGAT 1 cut(s) 46
BtsCI GGATG 2 cut(s) 216, 484
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 142, 523
CfoI GCGC 1 cut(s) 425
Cfr10I RCCGGY 1 cut(s) 521
ClaI ATCGAT 1 cut(s) 46
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 4 cut(s) 82, 117, 152, 187
CviAII CATG 1 cut(s) 218
CviQI GTAC 1 cut(s) 4
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 403
FaeI CATG 1 cut(s) 221
FaiI YATR 6 cut(s) 201, 219, 288, 411, 512, 564
FatI CATG 1 cut(s) 217
FauI CCCGC 1 cut(s) 518
Fnu4HI GCNGC 2 cut(s) 62, 434
FokI GGATG 2 cut(s) 223, 491
Fsp4HI GCNGC 2 cut(s) 62, 434
FspBI CTAG 1 cut(s) 294
GlaI GCGC 1 cut(s) 424
GluI GCNGC 2 cut(s) 62, 434
HaeIII GGCC 1 cut(s) 521
HapII CCGG 1 cut(s) 522
HhaI GCGC 1 cut(s) 425
Hin1II CATG 1 cut(s) 221
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HinfI GANTC 3 cut(s) 36, 43, 470
HpaII CCGG 1 cut(s) 522
Hpy166II GTNNAC 1 cut(s) 462
Hpy188I TCNGA 3 cut(s) 336, 441, 475
Hpy188III TCNNGA 3 cut(s) 40, 101, 403
Hpy8I GTNNAC 1 cut(s) 462
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 3 cut(s) 97, 171, 259
HpyCH4V TGCA 3 cut(s) 109, 181, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 150
Hsp92II CATG 1 cut(s) 221
HspAI GCGC 1 cut(s) 423
KroI GCCGGC 1 cut(s) 521
KroNI GCCGGC 1 cut(s) 523
Kzo9I GATC 1 cut(s) 55
LpnPI CCDG 7 cut(s) 18, 50, 191, 291, 369, 437, 535
Lsp1109I GCAGC 2 cut(s) 48, 445
LweI GCATC 2 cut(s) 420, 445
MaeI CTAG 1 cut(s) 294
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 3 cut(s) 5, 79, 506
MhlI GDGCHC 2 cut(s) 227, 276
MluCI AATT 3 cut(s) 104, 134, 250
MnlI CCTC 7 cut(s) 239, 369, 426, 469, 475, 478, 490
MroNI GCCGGC 1 cut(s) 521
MseI TTAA 2 cut(s) 261, 318
MspI CCGG 1 cut(s) 522
MvnI CGCG 2 cut(s) 144, 425
MwoI GCNNNNNNNGC 2 cut(s) 67, 150
NaeI GCCGGC 1 cut(s) 523
NdeII GATC 1 cut(s) 55
NgoMIV GCCGGC 1 cut(s) 521
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 3 cut(s) 69, 176, 275
PaeR7I CTCGAG 1 cut(s) 403
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PdiI GCCGGC 1 cut(s) 523
PfeI GAWTC 3 cut(s) 36, 43, 470
PkrI GCNGC 2 cut(s) 63, 435
PsiI TTATAA 1 cut(s) 564
PspN4I GGNNCC 3 cut(s) 69, 176, 275
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 261, 318
SatI GCNGC 2 cut(s) 62, 434
Sau3AI GATC 1 cut(s) 55
SduI GDGCHC 2 cut(s) 227, 276
SetI ASST 3 cut(s) 301, 410, 489
SfaNI GCATC 2 cut(s) 420, 445
Sfr274I CTCGAG 1 cut(s) 403
SlaI CTCGAG 1 cut(s) 403
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 3 cut(s) 104, 134, 250
SsiI CCGC 3 cut(s) 144, 425, 525
SspMI CTAG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 97, 171, 259
TaqI TCGA 4 cut(s) 39, 46, 365, 404
TasI AATT 3 cut(s) 104, 134, 250
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 3 cut(s) 36, 43, 470
Tru1I TTAA 2 cut(s) 261, 318
Tru9I TTAA 2 cut(s) 261, 318
TscAI CASTG 1 cut(s) 34
TseI GCWGC 2 cut(s) 61, 433
TspDTI ATGAA 1 cut(s) 527
TspRI CASTG 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 403
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.