Rw2G051250

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008673
Physical Location & Seq
Reverse (-)
78975626 .. 78976546
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051250.1

Sequence Viewer

Length: 921 bp
ATGGGCACATTCGAAGCCATCTCCTCCTCCACCCTCGACCCATCTCCACCGTCCACTCATCCCCAGTCGAAACCGCAAACGACCCAGTCCAAATCGTCGTTCATCTACTTCCTCCTCGCATCCCTTCTCTCCTTCCTCTTCATTCTCAACTCGTTGATTTCTTTCTTCGACGCCTTAAACTCAAACGATCGGGTCGGGTCGGCCCAGCAGCTCCAGGTCCTCGCGATTGCCGCCCTCTTCTTCCTCTACTCCATCACCGGCCTCCTGGTAAACTTCACCAACTCATCTTTCCCTTCTTCGCTGCTCAATTTGATCGTTCTCTTCGGTTTCGTTGAAGAATTCATGTACTTTTATCTTCAAAGAAAAGACGCTGCTGGAATCGAGAATCGATACTTTGATCTGCTGCTGGTGCCGATTTTCGTCTGTGTCTTCTCCACAGTTCTTGAATTGCACAACCCTAAATCGGTTTTCCCCAAATTGGCTCGCGGAGTTGGCTTGGTTCTACAAGGACTATGGTTCCTGCAAATGGGTCTTTCCATTTATACGCATTGGATGGCTCATGGGTGCTCATTGAAGGAAAAGAGTAGAGGGAATTACACGGTTAAGTGTAAGGGGCACCCTGAATATCATAGGGCTAGAGCTATCGCTACGCTTCAGTTTAACTGCCATTTGGCTCTGATAGTGGTTTTGGTGGTGGGGCTGTATTCGATTGTGGCGAGGAAAACAGGAGTTGTTGCTGATTTCTCGAGCTATAAGCCGCTTGGCGCGGAGATGCAGCATCAGATTGATAGTCAGGGGCAGTTTACTTTGGATTCGGAGGATGATAGTGATGGGGAGGTTAGAGAAGAGGGCAATGTGGTTATGAACAAGGCCGGCGGGGAAGTGGAGTTGGGTGTGAATGGAAATGGGACTTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

306

Amino Acids

33.84

Weight (kDa)

6.24

Isoelectric Point (pI)

34.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 100 - 235 9.1e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AccB1I GGYRCC 2 cut(s) 409, 615
AccII CGCG 3 cut(s) 224, 486, 767
AciI CCGC 6 cut(s) 74, 231, 486, 758, 767, 876
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 638
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 3 cut(s) 463, 478, 526
AgsI TTSAA 4 cut(s) 335, 359, 446, 574
AjnI CCWGG 2 cut(s) 213, 264
AjuI GAANNNNNNNTTGG 4 cut(s) 83, 115, 272, 304
AluBI AGCT 3 cut(s) 211, 641, 750
AluI AGCT 3 cut(s) 211, 641, 750
Alw21I GWGCWC 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 3 cut(s) 201, 259, 870
ApeKI GCWGC 5 cut(s) 208, 301, 371, 403, 775
ApoI RAATTY 1 cut(s) 338
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 2 cut(s) 202, 217
AsuHPI GGTGA 2 cut(s) 247, 268
AsuII TTCGAA 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 745
AvaII GGWCC 1 cut(s) 217
BaeGI GKGCMC 2 cut(s) 8, 618
BanI GGYRCC 2 cut(s) 409, 615
BbsI GAAGAC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 569
BbvI GCAGC 5 cut(s) 220, 288, 358, 390, 787
BccI CCATC 5 cut(s) 26, 49, 260, 547, 824
BciT130I CCWGG 2 cut(s) 215, 266
BfaI CTAG 1 cut(s) 636
BisI GCNGC 7 cut(s) 209, 231, 302, 372, 404, 758, 776
BlsI GCNGC 7 cut(s) 210, 232, 303, 373, 405, 759, 777
Bme1390I CCNGG 2 cut(s) 215, 266
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 202, 217
BmiI GGNNCC 3 cut(s) 411, 518, 617
BmrFI CCNGG 2 cut(s) 215, 266
BmrI ACTGGG 2 cut(s) 58, 79
BmsI GCATC 3 cut(s) 128, 762, 787
BmuI ACTGGG 2 cut(s) 58, 79
BpiI GAAGAC 1 cut(s) 421
BpmI CTGGAG 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 12
Bsa29I ATCGAT 1 cut(s) 388
BsaHI GRCGYC 1 cut(s) 171
Bsc4I CCNNNNNNNGG 3 cut(s) 463, 478, 526
Bse118I RCCGGY 2 cut(s) 257, 872
Bse1I ACTGG 2 cut(s) 64, 85
Bse3DI GCAATG 1 cut(s) 859
BseBI CCWGG 2 cut(s) 215, 266
BseCI ATCGAT 1 cut(s) 388
BseGI GGATG 4 cut(s) 58, 119, 558, 826
BseLI CCNNNNNNNGG 3 cut(s) 463, 478, 526
BseMI GCAATG 1 cut(s) 859
BseNI ACTGG 2 cut(s) 64, 85
BseRI GAGGAG 3 cut(s) 13, 16, 104
BseSI GKGCMC 2 cut(s) 8, 618
BseXI GCAGC 5 cut(s) 220, 288, 358, 390, 787
BseYI CCCAGC 1 cut(s) 204
Bsh1236I CGCG 3 cut(s) 224, 486, 767
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 203, 261, 872
BshNI GGYRCC 2 cut(s) 409, 615
BshVI ATCGAT 1 cut(s) 388
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 2 cut(s) 258, 873
BslI CCNNNNNNNGG 3 cut(s) 463, 478, 526
BsnI GGCC 3 cut(s) 203, 261, 872
BsoBI CYCGRG 1 cut(s) 745
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 3 cut(s) 8, 569, 618
Bsp143I GATC 3 cut(s) 187, 312, 397
Bsp68I TCGCGA 1 cut(s) 224
BspACI CCGC 6 cut(s) 74, 231, 486, 758, 767, 876
BspANI GGCC 3 cut(s) 203, 261, 872
BspDI ATCGAT 1 cut(s) 388
BspFNI CGCG 3 cut(s) 224, 486, 767
BspLI GGNNCC 3 cut(s) 411, 518, 617
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 409, 615
BsrDI GCAATG 1 cut(s) 859
BsrFI RCCGGY 2 cut(s) 257, 872
BsrI ACTGG 2 cut(s) 64, 85
BssAI RCCGGY 2 cut(s) 257, 872
BssMI GATC 3 cut(s) 187, 312, 397
BssNI GRCGYC 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 215, 266
Bst4CI ACNGT 3 cut(s) 51, 439, 601
Bst6I CTCTTC 4 cut(s) 143, 242, 326, 840
BstACI GRCGYC 1 cut(s) 171
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 484, 874
BstF5I GGATG 4 cut(s) 58, 119, 558, 826
BstFNI CGCG 3 cut(s) 224, 486, 767
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 3 cut(s) 190, 315, 400
BstMBI GATC 3 cut(s) 187, 312, 397
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 230, 409, 492
BstNI CCWGG 2 cut(s) 215, 266
BstSCI CCNGG 2 cut(s) 213, 264
BstSLI GKGCMC 2 cut(s) 8, 618
BstUI CGCG 3 cut(s) 224, 486, 767
BstV1I GCAGC 5 cut(s) 220, 288, 358, 390, 787
BstV2I GAAGAC 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 388
BsuRI GGCC 3 cut(s) 203, 261, 872
BsuTUI ATCGAT 1 cut(s) 388
BtsCI GGATG 4 cut(s) 58, 119, 558, 826
BtuMI TCGCGA 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 484, 874
CfoI GCGC 1 cut(s) 767
Cfr10I RCCGGY 2 cut(s) 257, 872
Cfr13I GGNCC 2 cut(s) 202, 217
ClaI ATCGAT 1 cut(s) 388
CseI GACGC 2 cut(s) 179, 377
Csp6I GTAC 1 cut(s) 346
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 2 cut(s) 343, 560
CviQI GTAC 1 cut(s) 346
DpnI GATC 3 cut(s) 189, 314, 399
DpnII GATC 3 cut(s) 187, 312, 397
Eam1104I CTCTTC 4 cut(s) 143, 242, 326, 840
EarI CTCTTC 4 cut(s) 143, 242, 326, 840
Eco47I GGWCC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 745
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 2 cut(s) 213, 264
FaeI CATG 2 cut(s) 346, 563
FaiI YATR 8 cut(s) 344, 514, 543, 561, 630, 753, 863, 915
FatI CATG 2 cut(s) 342, 559
FauI CCCGC 1 cut(s) 869
Fnu4HI GCNGC 7 cut(s) 209, 231, 302, 372, 404, 758, 776
FokI GGATG 4 cut(s) 45, 106, 565, 833
Fsp4HI GCNGC 7 cut(s) 209, 231, 302, 372, 404, 758, 776
FspBI CTAG 1 cut(s) 636
GlaI GCGC 1 cut(s) 766
GluI GCNGC 7 cut(s) 209, 231, 302, 372, 404, 758, 776
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 1 cut(s) 197
HaeIII GGCC 3 cut(s) 203, 261, 872
HapII CCGG 2 cut(s) 258, 873
HgaI GACGC 2 cut(s) 179, 377
HhaI GCGC 1 cut(s) 767
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 2 cut(s) 346, 563
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HinfI GANTC 3 cut(s) 378, 385, 812
HpaII CCGG 2 cut(s) 258, 873
HphI GGTGA 2 cut(s) 247, 268
Hpy166II GTNNAC 3 cut(s) 54, 271, 804
Hpy188I TCNGA 3 cut(s) 678, 783, 817
Hpy188III TCNNGA 4 cut(s) 223, 382, 443, 745
Hpy8I GTNNAC 3 cut(s) 54, 271, 804
Hpy99I CGWCG 2 cut(s) 100, 173
HpyAV CCTTC 4 cut(s) 134, 142, 303, 568
HpyCH4III ACNGT 3 cut(s) 51, 439, 601
HpyCH4V TGCA 3 cut(s) 451, 523, 775
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 409, 492
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 2 cut(s) 346, 563
HspAI GCGC 1 cut(s) 765
KroI GCCGGC 1 cut(s) 872
KroNI GCCGGC 1 cut(s) 874
Kzo9I GATC 3 cut(s) 187, 312, 397
LmnI GCTCC 1 cut(s) 216
Lsp1109I GCAGC 5 cut(s) 220, 288, 358, 390, 787
LweI GCATC 3 cut(s) 128, 762, 787
MaeI CTAG 1 cut(s) 636
MalI GATC 3 cut(s) 189, 314, 399
MboI GATC 3 cut(s) 187, 312, 397
MhlI GDGCHC 3 cut(s) 8, 569, 618
MluCI AATT 5 cut(s) 307, 338, 446, 476, 592
MroNI GCCGGC 1 cut(s) 872
MseI TTAA 3 cut(s) 176, 603, 660
MspI CCGG 2 cut(s) 258, 873
MspR9I CCNGG 2 cut(s) 215, 266
MvaI CCWGG 2 cut(s) 215, 266
MvnI CGCG 3 cut(s) 224, 486, 767
MwoI GCNNNNNNNGC 3 cut(s) 230, 409, 492
NaeI GCCGGC 1 cut(s) 874
NdeII GATC 3 cut(s) 187, 312, 397
NgoMIV GCCGGC 1 cut(s) 872
NlaIII CATG 2 cut(s) 346, 563
NlaIV GGNNCC 3 cut(s) 411, 518, 617
NruI TCGCGA 1 cut(s) 224
NspV TTCGAA 1 cut(s) 12
PaeR7I CTCGAG 1 cut(s) 745
PcsI WCGNNNNNNNCGW 1 cut(s) 713
PdiI GCCGGC 1 cut(s) 874
PfeI GAWTC 3 cut(s) 378, 385, 812
PflFI GACNNNGTC 1 cut(s) 85
PkrI GCNGC 7 cut(s) 210, 232, 303, 373, 405, 759, 777
Ple19I CGATCG 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 217
PsiI TTATAA 1 cut(s) 915
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 2 cut(s) 213, 264
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 2 cut(s) 213, 264
PspN4I GGNNCC 3 cut(s) 411, 518, 617
PspPI GGNCC 2 cut(s) 202, 217
PspPPI RGGWCCY 1 cut(s) 217
PsyI GACNNNGTC 1 cut(s) 85
PvuI CGATCG 1 cut(s) 190
RruI TCGCGA 1 cut(s) 224
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
SaqAI TTAA 3 cut(s) 176, 603, 660
SatI GCNGC 7 cut(s) 209, 231, 302, 372, 404, 758, 776
Sau3AI GATC 3 cut(s) 187, 312, 397
Sau96I GGNCC 2 cut(s) 202, 217
ScrFI CCNGG 2 cut(s) 215, 266
SduI GDGCHC 3 cut(s) 8, 569, 618
SetI ASST 5 cut(s) 213, 219, 643, 752, 840
SfaNI GCATC 3 cut(s) 128, 762, 787
Sfr274I CTCGAG 1 cut(s) 745
SfuI TTCGAA 1 cut(s) 12
SinI GGWCC 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 745
SmlI CTYRAG 1 cut(s) 745
SmoI CTYRAG 1 cut(s) 745
Sse9I AATT 5 cut(s) 307, 338, 446, 476, 592
SsiI CCGC 6 cut(s) 74, 231, 486, 758, 767, 876
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 213, 264
TaaI ACNGT 3 cut(s) 51, 439, 601
TaqI TCGA 8 cut(s) 12, 36, 68, 168, 381, 388, 707, 746
TasI AATT 5 cut(s) 307, 338, 446, 476, 592
TatI WGTACW 1 cut(s) 345
TauI GCSGC 2 cut(s) 233, 760
TfiI GAWTC 3 cut(s) 378, 385, 812
Tru1I TTAA 3 cut(s) 176, 603, 660
Tru9I TTAA 3 cut(s) 176, 603, 660
TseI GCWGC 5 cut(s) 208, 301, 371, 403, 775
TspDTI ATGAA 4 cut(s) 91, 130, 331, 878
Tth111I GACNNNGTC 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 1 cut(s) 338
XhoI CTCGAG 1 cut(s) 745
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.