RchiOBHm_Chr2g0170271

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008673
Physical Location & Seq
Reverse (-)
84240981 .. 84242912
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53782

Sequence Viewer

Length: 966 bp
ATGGGATTCTTCACCTTCGCTGCTGCAGGCGGCGGCCTGATTCTGATGGGCACATTGGAAGCCATCTCCTCCTCCACCGTCGACCCATCTCCACCGTCCACTCATCCCCAATCGAAACCGCAAAGGACCCAGTTCAAATCGTCGTTCATCTACTTCCTCCTCGCATCCATTCTCTCCTTCCTCTTCATTCTCAACTCGTTGGTTTCTTTCTTCGACGCCTTAAACTCAAACGATCGGGTCGGGTCAGCCCAGCAGCTCCAGGTCCTCGCGATTGCCGCCCTCTTCTTCCTCTACTCCATCACCGGCCTCCTGGTAAACTTCACCAACTCATCTTTCCCTTCTTCGCTGCTCAATTTGATCGTCCTCTTCGGTTTCGTTGAAGAATTCATGTACTTTTATCTTCAAAGAAAAGACACTGCTGGAATCGAGAATCGATACTTTGATCTGCTGCTGGTGCCGATTTTCGTCTGTGTCTTCTCCACAGTTCTTGAATTGCACAACCCTAAATCGGTTTTCCCCAAATTGGCTCGCGGAGTTGGCTTGGCTCTACAAGGACTGTGGTTCCTGCAAATGGGTCTTTCGATTTATACGCATTGGATGGCTCATGGGTGCTCATTGAAGGAAAAGAGTAGAGGGAATTACACGGTCAAGTGTAAGGGGCACCCTGAATATCATAGGGCTAGAGCCATCGCTACGCTTCAGTTTAACTGCCATTTGGCTCTGATAGTGGTTTTGGTGGTGGGGCTGTATTCGATTGTGGCGAGGAAAACAGGAGTTGTTGCTGATTTCTCGAGCTATAAGCCGCTTGGCGCGGAGATGCAGCATCAGATTGATAGTCAGGGGCAGTTTACTTTGGATTCGGAAGATGATAGTGATGAGGAGGTTAGAGAAGAGGGCAATGTGGTTATGAACAAGGCCGGCGGGGAAGTGGAGTTGGGTGTGAATGGTAATGGGACTTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

321

Amino Acids

35.42

Weight (kDa)

6.24

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 115 - 250 5.4e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
AccB1I GGYRCC 2 cut(s) 454, 660
AccI GTMKAC 1 cut(s) 81
AccII CGCG 3 cut(s) 269, 531, 812
AciI CCGC 8 cut(s) 30, 33, 119, 276, 531, 803, 812, 921
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 3 cut(s) 508, 523, 571
AgsI TTSAA 5 cut(s) 136, 380, 404, 491, 619
AjnI CCWGG 2 cut(s) 258, 309
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 2 cut(s) 256, 795
AluI AGCT 2 cut(s) 256, 795
Alw21I GWGCWC 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 790
AoxI GGCC 3 cut(s) 34, 304, 915
ApeKI GCWGC 6 cut(s) 20, 23, 253, 346, 448, 820
ApoI RAATTY 1 cut(s) 383
AspLEI GCGC 1 cut(s) 812
AspS9I GGNCC 2 cut(s) 126, 262
AsuHPI GGTGA 3 cut(s) 4, 292, 313
AvaI CYCGRG 1 cut(s) 790
AvaII GGWCC 2 cut(s) 126, 262
BaeGI GKGCMC 2 cut(s) 53, 663
BanI GGYRCC 2 cut(s) 454, 660
BbsI GAAGAC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 6 cut(s) 7, 10, 265, 333, 435, 832
BccI CCATC 6 cut(s) 40, 71, 94, 305, 592, 695
BciT130I CCWGG 2 cut(s) 260, 311
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 24
Bme1390I CCNGG 2 cut(s) 260, 311
Bme18I GGWCC 2 cut(s) 126, 262
BmeT110I CYCGRG 1 cut(s) 790
BmgT120I GGNCC 2 cut(s) 126, 262
BmiI GGNNCC 4 cut(s) 128, 456, 563, 662
BmrFI CCNGG 2 cut(s) 260, 311
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 3 cut(s) 173, 807, 832
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 433
BsaHI GRCGYC 1 cut(s) 216
Bsc4I CCNNNNNNNGG 3 cut(s) 508, 523, 571
Bse118I RCCGGY 2 cut(s) 302, 917
Bse1I ACTGG 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 904
BseBI CCWGG 2 cut(s) 260, 311
BseCI ATCGAT 1 cut(s) 433
BseGI GGATG 3 cut(s) 103, 164, 603
BseLI CCNNNNNNNGG 3 cut(s) 508, 523, 571
BseMI GCAATG 1 cut(s) 904
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 4 cut(s) 58, 61, 149, 893
BseSI GKGCMC 2 cut(s) 53, 663
BseXI GCAGC 6 cut(s) 7, 10, 265, 333, 435, 832
BseYI CCCAGC 1 cut(s) 249
Bsh1236I CGCG 3 cut(s) 269, 531, 812
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 3 cut(s) 36, 306, 917
BshNI GGYRCC 2 cut(s) 454, 660
BshVI ATCGAT 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 790
BsiSI CCGG 2 cut(s) 303, 918
BslI CCNNNNNNNGG 3 cut(s) 508, 523, 571
BsnI GGCC 3 cut(s) 36, 306, 917
BsoBI CYCGRG 1 cut(s) 790
Bsp1286I GDGCHC 3 cut(s) 53, 614, 663
Bsp143I GATC 3 cut(s) 232, 357, 442
Bsp68I TCGCGA 1 cut(s) 269
BspACI CCGC 8 cut(s) 30, 33, 119, 276, 531, 803, 812, 921
BspANI GGCC 3 cut(s) 36, 306, 917
BspDI ATCGAT 1 cut(s) 433
BspFNI CGCG 3 cut(s) 269, 531, 812
BspLI GGNNCC 4 cut(s) 128, 456, 563, 662
BspMAI CTGCAG 1 cut(s) 28
BspT107I GGYRCC 2 cut(s) 454, 660
BsrDI GCAATG 1 cut(s) 904
BsrFI RCCGGY 2 cut(s) 302, 917
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 2 cut(s) 302, 917
BssMI GATC 3 cut(s) 232, 357, 442
BssNI GRCGYC 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 260, 311
Bst4CI ACNGT 5 cut(s) 79, 96, 484, 558, 646
Bst6I CTCTTC 4 cut(s) 188, 287, 371, 885
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 28, 529, 919
BstF5I GGATG 3 cut(s) 103, 164, 603
BstFNI CGCG 3 cut(s) 269, 531, 812
BstHHI GCGC 1 cut(s) 812
BstKTI GATC 3 cut(s) 235, 360, 445
BstMBI GATC 3 cut(s) 232, 357, 442
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 275, 454, 537
BstNI CCWGG 2 cut(s) 260, 311
BstSCI CCNGG 2 cut(s) 258, 309
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 53, 663
BstUI CGCG 3 cut(s) 269, 531, 812
BstV1I GCAGC 6 cut(s) 7, 10, 265, 333, 435, 832
BstV2I GAAGAC 1 cut(s) 466
Bsu15I ATCGAT 1 cut(s) 433
BsuRI GGCC 3 cut(s) 36, 306, 917
BsuTUI ATCGAT 1 cut(s) 433
BtgZI GCGATG 1 cut(s) 673
BtsCI GGATG 3 cut(s) 103, 164, 603
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 28, 529, 919
CfoI GCGC 1 cut(s) 812
Cfr10I RCCGGY 2 cut(s) 302, 917
Cfr13I GGNCC 2 cut(s) 126, 262
ClaI ATCGAT 1 cut(s) 433
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 391
CspCI CAANNNNNGTGG 4 cut(s) 469, 504, 539, 574
CviAII CATG 2 cut(s) 388, 605
CviQI GTAC 1 cut(s) 391
DpnI GATC 3 cut(s) 234, 359, 444
DpnII GATC 3 cut(s) 232, 357, 442
Eam1104I CTCTTC 4 cut(s) 188, 287, 371, 885
EarI CTCTTC 4 cut(s) 188, 287, 371, 885
Eco47I GGWCC 2 cut(s) 126, 262
Eco57I CTGAAG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 790
EcoO109I RGGNCCY 2 cut(s) 126, 262
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 2 cut(s) 258, 309
FaeI CATG 2 cut(s) 391, 608
FaiI YATR 7 cut(s) 389, 588, 606, 675, 798, 908, 960
FatI CATG 2 cut(s) 387, 604
FauI CCCGC 1 cut(s) 914
FblI GTMKAC 1 cut(s) 81
FokI GGATG 3 cut(s) 90, 151, 610
FspBI CTAG 1 cut(s) 681
GlaI GCGC 1 cut(s) 811
GsaI CCCAGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 3 cut(s) 36, 306, 917
HapII CCGG 2 cut(s) 303, 918
HgaI GACGC 1 cut(s) 224
HhaI GCGC 1 cut(s) 812
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 391, 608
Hin6I GCGC 1 cut(s) 810
HinP1I GCGC 1 cut(s) 810
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 5 cut(s) 6, 40, 423, 430, 857
HpaII CCGG 2 cut(s) 303, 918
HphI GGTGA 3 cut(s) 4, 292, 313
Hpy166II GTNNAC 4 cut(s) 82, 99, 316, 849
Hpy188I TCNGA 4 cut(s) 45, 723, 828, 862
Hpy188III TCNNGA 4 cut(s) 268, 427, 488, 790
Hpy8I GTNNAC 4 cut(s) 82, 99, 316, 849
Hpy99I CGWCG 3 cut(s) 83, 145, 218
HpyAV CCTTC 4 cut(s) 25, 187, 348, 613
HpyCH4III ACNGT 5 cut(s) 79, 96, 484, 558, 646
HpyCH4V TGCA 4 cut(s) 26, 496, 568, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 454, 537
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 391, 608
HspAI GCGC 1 cut(s) 810
KroI GCCGGC 1 cut(s) 917
KroNI GCCGGC 1 cut(s) 919
Kzo9I GATC 3 cut(s) 232, 357, 442
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 6 cut(s) 7, 10, 265, 333, 435, 832
LweI GCATC 3 cut(s) 173, 807, 832
MaeI CTAG 1 cut(s) 681
MalI GATC 3 cut(s) 234, 359, 444
MboI GATC 3 cut(s) 232, 357, 442
MhlI GDGCHC 3 cut(s) 53, 614, 663
MluCI AATT 5 cut(s) 352, 383, 491, 521, 637
MroNI GCCGGC 1 cut(s) 917
MseI TTAA 2 cut(s) 221, 705
MspI CCGG 2 cut(s) 303, 918
MspR9I CCNGG 2 cut(s) 260, 311
MvaI CCWGG 2 cut(s) 260, 311
MvnI CGCG 3 cut(s) 269, 531, 812
MwoI GCNNNNNNNGC 3 cut(s) 275, 454, 537
NaeI GCCGGC 1 cut(s) 919
NdeII GATC 3 cut(s) 232, 357, 442
NgoMIV GCCGGC 1 cut(s) 917
NlaIII CATG 2 cut(s) 391, 608
NlaIV GGNNCC 4 cut(s) 128, 456, 563, 662
NruI TCGCGA 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 790
PcsI WCGNNNNNNNCGW 1 cut(s) 758
PdiI GCCGGC 1 cut(s) 919
PfeI GAWTC 5 cut(s) 6, 40, 423, 430, 857
Ple19I CGATCG 1 cut(s) 235
PpuMI RGGWCCY 2 cut(s) 126, 262
PsiI TTATAA 1 cut(s) 960
Psp5II RGGWCCY 2 cut(s) 126, 262
Psp6I CCWGG 2 cut(s) 258, 309
PspFI CCCAGC 1 cut(s) 249
PspGI CCWGG 2 cut(s) 258, 309
PspN4I GGNNCC 4 cut(s) 128, 456, 563, 662
PspPI GGNCC 2 cut(s) 126, 262
PspPPI RGGWCCY 2 cut(s) 126, 262
PstI CTGCAG 1 cut(s) 28
PvuI CGATCG 1 cut(s) 235
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 2 cut(s) 221, 705
Sau3AI GATC 3 cut(s) 232, 357, 442
Sau96I GGNCC 2 cut(s) 126, 262
ScrFI CCNGG 2 cut(s) 260, 311
SduI GDGCHC 3 cut(s) 53, 614, 663
SetI ASST 5 cut(s) 17, 258, 264, 797, 885
SfaNI GCATC 3 cut(s) 173, 807, 832
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 1 cut(s) 790
SinI GGWCC 2 cut(s) 126, 262
SlaI CTCGAG 1 cut(s) 790
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
Sse9I AATT 5 cut(s) 352, 383, 491, 521, 637
SsiI CCGC 8 cut(s) 30, 33, 119, 276, 531, 803, 812, 921
SspMI CTAG 1 cut(s) 681
StyD4I CCNGG 2 cut(s) 258, 309
TaaI ACNGT 5 cut(s) 79, 96, 484, 558, 646
TaqI TCGA 8 cut(s) 81, 113, 213, 426, 433, 581, 752, 791
TasI AATT 5 cut(s) 352, 383, 491, 521, 637
TatI WGTACW 1 cut(s) 390
TauI GCSGC 4 cut(s) 33, 36, 278, 805
TfiI GAWTC 5 cut(s) 6, 40, 423, 430, 857
Tru1I TTAA 2 cut(s) 221, 705
Tru9I TTAA 2 cut(s) 221, 705
TscAI CASTG 1 cut(s) 421
TseI GCWGC 6 cut(s) 20, 23, 253, 346, 448, 820
TspDTI ATGAA 4 cut(s) 136, 175, 376, 923
TspRI CASTG 1 cut(s) 421
VpaK11BI GGWCC 2 cut(s) 126, 262
XapI RAATTY 1 cut(s) 383
XhoI CTCGAG 1 cut(s) 790
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.