Rroxscaffold_2G00081480

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008673
Physical Location & Seq
Forward (+)
4533898 .. 4534864
967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081480.1

Sequence Viewer

Length: 897 bp
ATGGGATTCTTCACCTTCGCAGCCGCAGGCGGCGGCCTGATTCTGATGGGCACATTCGAAGCCATCTCCTCCTCCACCGTCGACCCATCTCCACCGTCCACTCATCCCCAATCGAAACCGCAAACGACCCAGTCCAAATCGTCGTTCATCTACTTCCTCCTCGCATCCCTTCTCTCCTTCCTCTTCATTCTCAACTCGTTGATTTCCTTCTTCGACGCCTTAAACTCAAACGATCGGGTCGGGTCGGCCCAGCAGCTCCAGGTCATCGCGATCGCCGCCCTCTTCTTCCTCTACTCCATCACCGGCCTCCTGAATTCATGTTCTTTTTATCTTCAAAGAAAAGACACTGCTGGAATCGAGAATCGATACTTTGATCTGTTGCTGGTGCCGATTTTCGTCTGTGTGTTCTCCACAGTGCTTGAATTGCACAACCCTAAATCGATTTTCCCCAAACTGGCTCGCGGAGTTGGCTTGGTTCTACAAGGACTGTGGTTCTTGCAAATGGGTCTTTCGATTTATACGCATTGGATGGCTCATGGGTGCTCATTGAAGGAAAAGAGTAGAGGGAATTACACGGTTAAGTGTAAGGGGCACCCTGAATATCACAGGGCTAGAGCTATTGCTACGCTTCAGTTTAACTGCCATTTGGCTCTGATAGTGGTTTTGGTGGTGGGGGTGTATTCGATTGTGGCGAGGAAAACAAGAGTTGTTGCTGATTTCTCGAGCTATAAGCCGCTTGGCGCGGAAATGCAGCGTCAGATTGATAGTCAGGGGCAGTTTACTTTGGATTCGGAGGATGATGGTGATGAGGAGGTTAGAGAAGAGGGCAATGTGGTTATGAACAAGGCCGGCGGGGAAGTGGAGTTGGGTGTGAATGGTAATGGGACTTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

298

Amino Acids

32.76

Weight (kDa)

7.04

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 99 - 227 1.7e-41 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 891
AccB1I GGYRCC 2 cut(s) 385, 591
AccI GTMKAC 1 cut(s) 81
AccII CGCG 3 cut(s) 269, 462, 743
AciI CCGC 9 cut(s) 24, 30, 33, 119, 276, 462, 734, 743, 852
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 614
AcyI GRCGYC 1 cut(s) 216
AfiI CCNNNNNNNGG 1 cut(s) 454
AgsI TTSAA 3 cut(s) 335, 422, 550
AjnI CCWGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 3 cut(s) 256, 617, 726
AluI AGCT 3 cut(s) 256, 617, 726
Alw21I GWGCWC 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 721
AoxI GGCC 4 cut(s) 34, 246, 304, 846
ApeKI GCWGC 3 cut(s) 20, 253, 751
ApoI RAATTY 1 cut(s) 313
AsiSI GCGATCGC 1 cut(s) 273
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 4, 292, 815
AsuII TTCGAA 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 721
BaeGI GKGCMC 2 cut(s) 53, 594
BanI GGYRCC 2 cut(s) 385, 591
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 3 cut(s) 32, 265, 763
BccI CCATC 6 cut(s) 40, 71, 94, 305, 523, 794
BciT130I CCWGG 1 cut(s) 260
BfaI CTAG 1 cut(s) 612
BisI GCNGC 8 cut(s) 21, 24, 31, 34, 254, 276, 734, 752
BlsI GCNGC 8 cut(s) 22, 25, 32, 35, 255, 277, 735, 753
Bme1390I CCNGG 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 721
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 387, 593
BmrFI CCNGG 1 cut(s) 260
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 173
BmuI ACTGGG 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 57
Bsa29I ATCGAT 2 cut(s) 364, 440
BsaHI GRCGYC 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse118I RCCGGY 2 cut(s) 302, 848
Bse1I ACTGG 2 cut(s) 130, 459
Bse3DI GCAATG 1 cut(s) 835
BseBI CCWGG 1 cut(s) 260
BseCI ATCGAT 2 cut(s) 364, 440
BseGI GGATG 4 cut(s) 103, 164, 534, 802
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMI GCAATG 1 cut(s) 835
BseNI ACTGG 2 cut(s) 130, 459
BseRI GAGGAG 4 cut(s) 58, 61, 149, 824
BseSI GKGCMC 2 cut(s) 53, 594
BseXI GCAGC 3 cut(s) 32, 265, 763
BseYI CCCAGC 1 cut(s) 249
Bsh1236I CGCG 3 cut(s) 269, 462, 743
Bsh1285I CGRYCG 2 cut(s) 235, 273
BshFI GGCC 4 cut(s) 36, 248, 306, 848
BshNI GGYRCC 2 cut(s) 385, 591
BshVI ATCGAT 2 cut(s) 364, 440
BsiEI CGRYCG 2 cut(s) 235, 273
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 721
BsiSI CCGG 2 cut(s) 303, 849
BslI CCNNNNNNNGG 1 cut(s) 454
BsnI GGCC 4 cut(s) 36, 248, 306, 848
BsoBI CYCGRG 1 cut(s) 721
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 3 cut(s) 53, 545, 594
Bsp143I GATC 3 cut(s) 232, 270, 373
Bsp68I TCGCGA 1 cut(s) 269
BspACI CCGC 9 cut(s) 24, 30, 33, 119, 276, 462, 734, 743, 852
BspANI GGCC 4 cut(s) 36, 248, 306, 848
BspDI ATCGAT 2 cut(s) 364, 440
BspFNI CGCG 3 cut(s) 269, 462, 743
BspLI GGNNCC 2 cut(s) 387, 593
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 2 cut(s) 385, 591
BsrDI GCAATG 1 cut(s) 835
BsrFI RCCGGY 2 cut(s) 302, 848
BsrI ACTGG 2 cut(s) 130, 459
BssAI RCCGGY 2 cut(s) 302, 848
BssMI GATC 3 cut(s) 232, 270, 373
BssNI GRCGYC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 5 cut(s) 79, 96, 415, 489, 577
Bst6I CTCTTC 3 cut(s) 188, 287, 816
BstACI GRCGYC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 28, 460, 850
BstF5I GGATG 4 cut(s) 103, 164, 534, 802
BstFNI CGCG 3 cut(s) 269, 462, 743
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 3 cut(s) 235, 273, 376
BstMBI GATC 3 cut(s) 232, 270, 373
BstMCI CGRYCG 2 cut(s) 235, 273
BstMWI GCNNNNNNNGC 3 cut(s) 275, 424, 468
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 258
BstSLI GKGCMC 2 cut(s) 53, 594
BstUI CGCG 3 cut(s) 269, 462, 743
BstV1I GCAGC 3 cut(s) 32, 265, 763
Bsu15I ATCGAT 2 cut(s) 364, 440
BsuRI GGCC 4 cut(s) 36, 248, 306, 848
BsuTUI ATCGAT 2 cut(s) 364, 440
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 4 cut(s) 103, 164, 534, 802
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 345, 420
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 28, 460, 850
CfoI GCGC 1 cut(s) 743
Cfr10I RCCGGY 2 cut(s) 302, 848
Cfr13I GGNCC 1 cut(s) 247
ClaI ATCGAT 2 cut(s) 364, 440
CseI GACGC 2 cut(s) 224, 743
CspCI CAANNNNNGTGG 4 cut(s) 400, 435, 470, 505
CviAII CATG 2 cut(s) 318, 536
DpnI GATC 3 cut(s) 234, 272, 375
DpnII GATC 3 cut(s) 232, 270, 373
Eam1104I CTCTTC 3 cut(s) 188, 287, 816
EarI CTCTTC 3 cut(s) 188, 287, 816
Eco57I CTGAAG 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 721
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 258
FaeI CATG 2 cut(s) 321, 539
FaiI YATR 6 cut(s) 319, 519, 537, 729, 839, 891
FatI CATG 2 cut(s) 317, 535
FauI CCCGC 1 cut(s) 845
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 8 cut(s) 21, 24, 31, 34, 254, 276, 734, 752
FokI GGATG 4 cut(s) 90, 151, 541, 809
Fsp4HI GCNGC 8 cut(s) 21, 24, 31, 34, 254, 276, 734, 752
FspBI CTAG 1 cut(s) 612
GlaI GCGC 1 cut(s) 742
GluI GCNGC 8 cut(s) 21, 24, 31, 34, 254, 276, 734, 752
GsaI CCCAGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 4 cut(s) 36, 248, 306, 848
HapII CCGG 2 cut(s) 303, 849
HgaI GACGC 2 cut(s) 224, 743
HhaI GCGC 1 cut(s) 743
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 321, 539
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 5 cut(s) 6, 40, 354, 361, 788
HpaII CCGG 2 cut(s) 303, 849
HphI GGTGA 3 cut(s) 4, 292, 815
Hpy166II GTNNAC 3 cut(s) 82, 99, 780
Hpy188I TCNGA 4 cut(s) 45, 654, 759, 793
Hpy188III TCNNGA 4 cut(s) 268, 310, 358, 721
Hpy8I GTNNAC 3 cut(s) 82, 99, 780
Hpy99I CGWCG 3 cut(s) 83, 145, 218
HpyAV CCTTC 5 cut(s) 25, 179, 187, 217, 544
HpyCH4III ACNGT 5 cut(s) 79, 96, 415, 489, 577
HpyCH4V TGCA 3 cut(s) 427, 499, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 424, 468
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 321, 539
HspAI GCGC 1 cut(s) 741
KroI GCCGGC 1 cut(s) 848
KroNI GCCGGC 1 cut(s) 850
Kzo9I GATC 3 cut(s) 232, 270, 373
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 3 cut(s) 32, 265, 763
LweI GCATC 1 cut(s) 173
MaeI CTAG 1 cut(s) 612
MalI GATC 3 cut(s) 234, 272, 375
MboI GATC 3 cut(s) 232, 270, 373
MboII GAAGA 6 cut(s) 175, 202, 274, 277, 323, 833
MhlI GDGCHC 3 cut(s) 53, 545, 594
MluCI AATT 3 cut(s) 313, 422, 568
MroNI GCCGGC 1 cut(s) 848
MseI TTAA 3 cut(s) 221, 579, 636
MspI CCGG 2 cut(s) 303, 849
MspR9I CCNGG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 3 cut(s) 269, 462, 743
MwoI GCNNNNNNNGC 3 cut(s) 275, 424, 468
NaeI GCCGGC 1 cut(s) 850
NdeII GATC 3 cut(s) 232, 270, 373
NgoMIV GCCGGC 1 cut(s) 848
NlaIII CATG 2 cut(s) 321, 539
NlaIV GGNNCC 2 cut(s) 387, 593
NruI TCGCGA 1 cut(s) 269
NspV TTCGAA 1 cut(s) 57
PaeR7I CTCGAG 1 cut(s) 721
PcsI WCGNNNNNNNCGW 1 cut(s) 689
PdiI GCCGGC 1 cut(s) 850
PfeI GAWTC 5 cut(s) 6, 40, 354, 361, 788
PflFI GACNNNGTC 1 cut(s) 130
PkrI GCNGC 8 cut(s) 22, 25, 32, 35, 255, 277, 735, 753
Ple19I CGATCG 2 cut(s) 235, 273
PsiI TTATAA 1 cut(s) 891
Psp6I CCWGG 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 249
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 387, 593
PspPI GGNCC 1 cut(s) 247
PsyI GACNNNGTC 1 cut(s) 130
PvuI CGATCG 2 cut(s) 235, 273
RgaI GCGATCGC 1 cut(s) 273
RruI TCGCGA 1 cut(s) 269
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 221, 579, 636
SatI GCNGC 8 cut(s) 21, 24, 31, 34, 254, 276, 734, 752
Sau3AI GATC 3 cut(s) 232, 270, 373
Sau96I GGNCC 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 3 cut(s) 53, 545, 594
SetI ASST 6 cut(s) 17, 258, 264, 619, 728, 816
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 1 cut(s) 173
Sfr274I CTCGAG 1 cut(s) 721
SfuI TTCGAA 1 cut(s) 57
SgfI GCGATCGC 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 721
SmlI CTYRAG 1 cut(s) 721
SmoI CTYRAG 1 cut(s) 721
Sse9I AATT 3 cut(s) 313, 422, 568
SsiI CCGC 9 cut(s) 24, 30, 33, 119, 276, 462, 734, 743, 852
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 258
TaaI ACNGT 5 cut(s) 79, 96, 415, 489, 577
TasI AATT 3 cut(s) 313, 422, 568
TauI GCSGC 5 cut(s) 26, 33, 36, 278, 736
TfiI GAWTC 5 cut(s) 6, 40, 354, 361, 788
Tru1I TTAA 3 cut(s) 221, 579, 636
Tru9I TTAA 3 cut(s) 221, 579, 636
TscAI CASTG 2 cut(s) 352, 420
TseI GCWGC 3 cut(s) 20, 253, 751
TspDTI ATGAA 4 cut(s) 136, 175, 306, 854
TspRI CASTG 2 cut(s) 352, 420
Tth111I GACNNNGTC 1 cut(s) 130
XapI RAATTY 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.