pycom17g03660

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008673
Physical Location & Seq
Forward (+)
2445077 .. 2446127
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03660.1

Sequence Viewer

Length: 993 bp
ATGGGGTTTCTCACCTTCGCCGCCGCAGGCGGCGGCCTGATCTTGATAGGCGCGTACGAAGCCATTTCCTCCTCAATCCAAAACCCAGATTCAGATTCATCTCCGCCGTCGCCGCCTCAACCTCCGATTCGTCCCCCGATGGCGCAGTCGAAAGCGAAAACCCAGTCTCTGTCGTCGTCGATTTATTACCTGGCCGCCGCCGTTATTTCGTTGCTGTTCATCTTGAACTCTTTGGTTTCGTTCTTTGACGCGCTCGACTCAGCGGATCCAGTCGGCTCAGCTATGCAATTGCAAGTGCTGGCGATCGCGTCGCTGTTCTTGCTCTACGCAATTACGGGTCTTGTGGTAAATTTCACGAATTCAGCCCTACCTTGTTCGTTGCTTAGTTTGGTCGGTCTTTTCGCTTTTGTTGAAGAATTTTTAATGTTTTACATGCAAAGAAAAGACACTTTCGGAATCGAAAATCGGTACTTTGATCTGCTGCTTGTGCCGATTGCTGTGTGTATATTTTCTACAATGCTTGAATTCAACAACCCCAAATCGAATTATCCGAAATTGGCTCGTGGGGTTGGGCTGGTTCTGCAGGGAATGTGGTTTCTGCAAATGGGTATTTCTTTGTACACCAACTTCGTGGCGCACGGATGCTCGTTGCACGAAAAGAGTAGAGGGAATTACACTGTGAAATGCAAGGGGCACCCTGAGTATCACAGGGCTAGAGCCATCGTCACGCTTCAGTTTAACTGCCATCTTGCTTTTATGGTGGTTGTGGTTGTGGGAGTGTACTCGATTATTGCTAGGAAGCGTGGCGGATTTCAGGCCAATTTACTTTGGATTCTGATGATGGCGATGATGAGATTAAAGAGGAGGGGAATTCGGCAATGCAGAAAGCCGGCGAAGTGGAATTGGGTGTCAACGGTTATGGTTCTCATAAGTGAGTATATCTTACCAAATTTTGATATGCTGAATGTAGAACTTCATTTTTTGAACCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

331

Amino Acids

36.67

Weight (kDa)

9.3

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 126 - 261 2.5e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 693
AccII CGCG 3 cut(s) 53, 251, 308
AclWI GGATC 2 cut(s) 260, 273
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 6 cut(s) 349, 358, 416, 524, 870, 949
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 4 cut(s) 56, 470, 620, 782
AgsI TTSAA 5 cut(s) 226, 413, 524, 529, 985
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 2 cut(s) 260, 273
AlwNI CAGNNNCTG 1 cut(s) 169
AoxI GGCC 3 cut(s) 34, 192, 816
ApeKI GCWGC 1 cut(s) 481
ApoI RAATTY 6 cut(s) 349, 358, 416, 524, 870, 949
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AsiSI GCGATCGC 1 cut(s) 306
AspLEI GCGC 4 cut(s) 53, 145, 253, 637
AsuHPI GGTGA 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 696
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 693
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BauI CACGAG 1 cut(s) 561
BbvI GCAGC 1 cut(s) 468
BccI CCATC 4 cut(s) 133, 728, 753, 835
BceAI ACGGC 2 cut(s) 91, 185
BciT130I CCWGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 2 cut(s) 714, 795
BfmI CTRYAG 1 cut(s) 581
BisI GCNGC 8 cut(s) 21, 24, 31, 34, 113, 195, 198, 482
BlpI GCTNAGC 1 cut(s) 277
BlsI GCNGC 8 cut(s) 22, 25, 32, 35, 114, 196, 199, 483
Bme1390I CCNGG 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 267, 695
BmrFI CCNGG 1 cut(s) 191
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 632
BmuI ACTGGG 1 cut(s) 157
Bpu1102I GCTNAGC 1 cut(s) 277
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 2 cut(s) 163, 269
Bse3DI GCAATG 1 cut(s) 884
BseBI CCWGG 1 cut(s) 191
BseGI GGATG 1 cut(s) 647
BseMI GCAATG 1 cut(s) 884
BseMII CTCAG 3 cut(s) 273, 291, 690
BseNI ACTGG 2 cut(s) 163, 269
BseRI GAGGAG 2 cut(s) 61, 877
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 1 cut(s) 468
Bsh1236I CGCG 3 cut(s) 53, 251, 308
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 36, 194, 818
BshNI GGYRCC 1 cut(s) 693
BsiEI CGRYCG 1 cut(s) 306
BsiSI CCGG 1 cut(s) 890
BsiWI CGTACG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 117
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 3 cut(s) 36, 194, 818
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 4 cut(s) 39, 265, 303, 475
Bsp1720I GCTNAGC 1 cut(s) 277
BspANI GGCC 3 cut(s) 36, 194, 818
BspCNI CTCAG 3 cut(s) 272, 290, 691
BspFNI CGCG 3 cut(s) 53, 251, 308
BspLI GGNNCC 2 cut(s) 267, 695
BspMAI CTGCAG 1 cut(s) 585
BspPI GGATC 2 cut(s) 260, 273
BspT107I GGYRCC 1 cut(s) 693
BsrDI GCAATG 1 cut(s) 884
BsrFI RCCGGY 1 cut(s) 889
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 2 cut(s) 163, 269
BssAI RCCGGY 1 cut(s) 889
BssMI GATC 4 cut(s) 39, 265, 303, 475
BssSI CACGAG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 679, 916
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 28, 300, 891
BstDEI CTNAG 5 cut(s) 259, 277, 383, 699, 990
BstF5I GGATG 1 cut(s) 647
BstFNI CGCG 3 cut(s) 53, 251, 308
BstHHI GCGC 4 cut(s) 53, 145, 253, 637
BstKTI GATC 4 cut(s) 42, 268, 306, 478
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 4 cut(s) 39, 265, 303, 475
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 5 cut(s) 59, 112, 319, 487, 580
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 581
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 3 cut(s) 53, 251, 308
BstV1I GCAGC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 265
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 3 cut(s) 36, 194, 818
BtgZI GCGATG 1 cut(s) 860
BtsCI GGATG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 675
Cac8I GCNNGC 3 cut(s) 28, 300, 891
CaiI CAGNNNCTG 1 cut(s) 169
CfoI GCGC 4 cut(s) 53, 145, 253, 637
Cfr10I RCCGGY 1 cut(s) 889
CseI GACGC 2 cut(s) 257, 297
Csp6I GTAC 4 cut(s) 55, 469, 619, 781
CviAII CATG 1 cut(s) 433
CviQI GTAC 4 cut(s) 55, 469, 619, 781
DdeI CTNAG 5 cut(s) 259, 277, 383, 699, 990
DpnI GATC 4 cut(s) 41, 267, 305, 477
DpnII GATC 4 cut(s) 39, 265, 303, 475
EaeI YGGCCR 1 cut(s) 192
EciI GGCGGA 2 cut(s) 93, 822
Eco57I CTGAAG 1 cut(s) 716
EcoRI GAATTC 3 cut(s) 358, 524, 870
EcoRII CCWGG 1 cut(s) 189
FaeI CATG 1 cut(s) 436
FaiI YATR 8 cut(s) 284, 434, 506, 758, 920, 929, 939, 959
FaqI GGGAC 1 cut(s) 117
FatI CATG 1 cut(s) 432
Fnu4HI GCNGC 8 cut(s) 21, 24, 31, 34, 113, 195, 198, 482
FokI GGATG 1 cut(s) 654
Fsp4HI GCNGC 8 cut(s) 21, 24, 31, 34, 113, 195, 198, 482
FspBI CTAG 2 cut(s) 714, 795
GlaI GCGC 4 cut(s) 52, 144, 252, 636
GluI GCNGC 8 cut(s) 21, 24, 31, 34, 113, 195, 198, 482
HaeIII GGCC 3 cut(s) 36, 194, 818
HapII CCGG 1 cut(s) 890
HgaI GACGC 2 cut(s) 257, 297
HhaI GCGC 4 cut(s) 53, 145, 253, 637
Hin1II CATG 1 cut(s) 436
Hin6I GCGC 4 cut(s) 51, 143, 251, 635
HinP1I GCGC 4 cut(s) 51, 143, 251, 635
HincII GTYRAC 1 cut(s) 912
HindII GTYRAC 1 cut(s) 912
HinfI GANTC 6 cut(s) 89, 95, 127, 257, 456, 832
HpaII CCGG 1 cut(s) 890
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 3 cut(s) 621, 781, 912
Hpy188I TCNGA 5 cut(s) 94, 126, 455, 552, 837
Hpy188III TCNNGA 3 cut(s) 43, 223, 355
Hpy8I GTNNAC 3 cut(s) 621, 781, 912
Hpy99I CGWCG 4 cut(s) 112, 178, 181, 313
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 2 cut(s) 679, 916
HpyCH4V TGCA 8 cut(s) 286, 292, 436, 583, 601, 652, 687, 882
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 112, 319, 487, 580
HpyF3I CTNAG 5 cut(s) 259, 277, 383, 699, 990
Hsp92II CATG 1 cut(s) 436
HspAI GCGC 4 cut(s) 51, 143, 251, 635
KroI GCCGGC 1 cut(s) 889
KroNI GCCGGC 1 cut(s) 891
Kzo9I GATC 4 cut(s) 39, 265, 303, 475
Lsp1109I GCAGC 1 cut(s) 468
LweI GCATC 1 cut(s) 632
MaeI CTAG 2 cut(s) 714, 795
MaeIII GTNAC 1 cut(s) 724
MalI GATC 4 cut(s) 41, 267, 305, 477
MboI GATC 4 cut(s) 39, 265, 303, 475
MboII GAAGA 1 cut(s) 425
MfeI CAATTG 1 cut(s) 287
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 696
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 7 cut(s) 79, 82, 126, 132, 659, 855, 858
MroNI GCCGGC 1 cut(s) 889
MseI TTAA 3 cut(s) 422, 738, 857
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 890
MspR9I CCNGG 1 cut(s) 191
MunI CAATTG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 3 cut(s) 53, 251, 308
MwoI GCNNNNNNNGC 5 cut(s) 59, 112, 319, 487, 580
NaeI GCCGGC 1 cut(s) 891
NdeII GATC 4 cut(s) 39, 265, 303, 475
NgoMIV GCCGGC 1 cut(s) 889
NlaIII CATG 1 cut(s) 436
NlaIV GGNNCC 2 cut(s) 267, 695
NmuCI GTSAC 1 cut(s) 724
NspI RCATGY 1 cut(s) 436
PcsI WCGNNNNNNNCGW 1 cut(s) 548
PdiI GCCGGC 1 cut(s) 891
PfeI GAWTC 5 cut(s) 89, 95, 127, 456, 832
Pfl23II CGTACG 1 cut(s) 54
PkrI GCNGC 8 cut(s) 22, 25, 32, 35, 114, 196, 199, 483
Ple19I CGATCG 1 cut(s) 306
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspLI CGTACG 1 cut(s) 54
PspN4I GGNNCC 2 cut(s) 267, 695
PstI CTGCAG 1 cut(s) 585
PstNI CAGNNNCTG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 265
PvuI CGATCG 1 cut(s) 306
RgaI GCGATCGC 1 cut(s) 306
RsaI GTAC 4 cut(s) 56, 470, 620, 782
RsaNI GTAC 4 cut(s) 55, 469, 619, 781
SaqAI TTAA 3 cut(s) 422, 738, 857
SatI GCNGC 8 cut(s) 21, 24, 31, 34, 113, 195, 198, 482
Sau3AI GATC 4 cut(s) 39, 265, 303, 475
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 696
SetI ASST 5 cut(s) 17, 124, 192, 283, 373
SfaAI GCGATCGC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 632
SfcI CTRYAG 1 cut(s) 581
SgfI GCGATCGC 1 cut(s) 306
SspMI CTAG 2 cut(s) 714, 795
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 2 cut(s) 679, 916
TaqI TCGA 6 cut(s) 149, 179, 255, 459, 542, 785
TaqII GACCGA 1 cut(s) 383
TatI WGTACW 2 cut(s) 618, 780
TauI GCSGC 7 cut(s) 23, 26, 33, 36, 115, 197, 200
TfiI GAWTC 5 cut(s) 89, 95, 127, 456, 832
Tru1I TTAA 3 cut(s) 422, 738, 857
Tru9I TTAA 3 cut(s) 422, 738, 857
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 1 cut(s) 481
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 3 cut(s) 87, 208, 965
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 1 cut(s) 682
XapI RAATTY 6 cut(s) 349, 358, 416, 524, 870, 949
XceI RCATGY 1 cut(s) 436
XspI CTAG 2 cut(s) 714, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.