Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003013

Physical Location & Seq

Genomic Coordinates Reverse (-)

4492099 .. 4498389

Gene Structure 6291 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1066000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2895 bp

ATGGCAGTTGGGGTTATTCACCACTGCCCCATTGCATCTTCAGGTCCACATTGCAAGTATGATAGGAGTGTATGCCGCCTGGGGCGGCGTCCTCTTGTCCTCAGAACTAATTCGAATAGAAAAAAGAACCTTTTCTACACGCCACATTGGCTATGTAAATCAAGAACTAGTATTTTTTCAAGCATGGATGATTCCACTGACACTTTTACCAACGTGGCTAACACTTCGGGTAGTAGTGAAGTGTTGAATATAGAAGAAGATGAAATGATGACCGCTAGGAAAGCTCTTTTGGAGGCCCAAGCTAGACAAGGAGCCATTGAGAAAGAGAGAGATCAATTGCTTGAAGAGTTAGCATGTTCCGAGGCTAAACAACAGGAATATGTTGCGACCATATTGCACGACAAGGAATTGGCTATCGCAGAACTCGAGGCAGCCAAGTCACTGTTCCATCAGAAGCTGCTGGAATCAGTTGAAGAGAAGTTCAGCTTAGAGTCTAAGTTAGTTCTTGCAAAACAGGATGCAGTTGAACTTGCGGTACAAGTAGAAAAGTTAGCGGAAATTGCTTTCCAGCAGGCCACATCTCACATTCTACAAGATGCCCAGATGAGGGTTTCGGCAGCAGAAACTACAGCTGCTGAAGCAGCTTATCAGATAGAAAAACAAATTAAGGAGGTGACTGAAGGTAGTATATTGTTAATTGTGGAACAGTCAAAACTTGCAATAGAGAAGGCACTGGATGCGGCGGAAAAATCTGGTGAGCATGCATCAAAAGCAGTATTAGAATATACTGAAGGTGTGAGTCCACTGGATGAGCTTGCTTCCCTTCAGTCAAAAAATATTATGCTACAGGGTGCCGTAAATGATTTAGAATCTCAGTTGTTGCTTACAAGAAGTGACGTTGATAGGTTGAAGTTGGAGTTGGAAAAGGCCCTTGCACATGCAAATGCGTTTGAGGTCCGAGCTAATGATGCTGAGAAAGCATTGCTTGAATTTCAGGAATCCAGTAGGAAAAATACCCTCCAGAAAGAGGAGGAAATTATGTCTTTGATAGAGAAAATGAAGAAAGATACGTCAGAAAGAATGAAGTCTTCTTCTAAGGCTTTCAAGGCTGAGTTGCAAAGCATTAGGGATGCTATTGGAGCTGCCAAAGAAATGGCATGTACGAAAGATGATGCATACTTGAGAAGATGTGAAGCACTGCGAAGATCACTGAAGGCATCTGAAGCTGCTACAAAGATGTGGAGACAGAGAGCAGAAATGGCAGAATCATTATTATTGAAGGACAGATCACTGGGTGAAGGGGATGAAGATTCTATTTATGTTGTTAATGGTGGGCGGATAGACCTTTTGACAGACGATGATTCACAGAAATGGAAACTTTTAAGCGATGGCCCTCGCCGGGAGATACCTCAATGGATGGCTAGGAAAATTCGTACTATCAGTCCCAGGTTCCCTCCAAGAAAGATCGATGTAGCTGAAGCCTCGAGTTCAAAGTTCAGATCTTTGAATTTGCCCAAACCTGATGAAGTATGGTCTATAGCTAAGGAAAAGCCAAAGGAGGGCGATACACTTATTGAGCATGTGCGTGAGAGAGAAACAATAGAAAAGAAACGAAAAGCTCTGGAGCACGTTCTTCAGAGGAAGACCATACAATGGCAGAGCACCCAGGAACAAACAAAGTTAGAGCCAGGAACTGGAACCGGACGTGAGATCGTGTTTCAAGGCTTCAATTGGGAAAGCTGGAGAAGGCAGTGGTACTTAGACTTAGCTCCTAAAGCTGCTGATTTATCTAAAATTGGGGTAACGGCAGTGTGGCTCCCACCACCAACAGAATCTGTCGCTCCTCAAGGTTATATGCCTTCGGACCTTTACAATTTGAACTCAGCATATGGTACTGTGGATGAACTTAAACACTGCATTGAGGAAATGCATTCTCAAGATCTTCTGGCTCTGGGAGATGTTGTCCTAAATCATAGATGTGCTCATAAACAGAGTCCAAACGGCATTTGGAACATTTTTGGTGGGAAGCTTGCTTGGGGGCCTGAAGCAATTGTTTGTGATGATCCAAATTTTCAGGGCCAAGGAAATCCTTCAAGTGGGGATATATTCCATGCGGCACCCAATATTGATCATTCGAAGGAATTTGTAAGAAATGATATAAAGGAGTGGCTAAACTGGCTTCGGAATGATATTGGTTTTGATGGATGGCGCCTTGATTTCGTAAGAGGCTTCTCTGGCACCTATGTAAAAGAATATATTGAAGCTTCGGTTCCTGCATTTGCTATTGGAGAATATTGGGACAGCTTAGCTTATGAACATGGAAATTTGTGTTACAATCAAGATGCTCACCGGCAACGGATTGTCAATTGGATCAATGCCACAGGCGGCACTTCCTCGGCATTTGATGTCACAACAAAGGGAATACTCCACTCTGCTCTTCATAACCAATATTGGAGGCTAATAGATCCTCAGGGTAAACCGACTGGAGTTTTGGGATGGTGGCCCTCTCGTGCTGTCACGTTTTTGGAGAACCACGACACAGGATCAACACAGGGTCACTGGCCGTTTCCACGAGATAAGCTTACACAGGGATACGCGTACATCCTAACTCATCCCGGAACACCTGTCATTTTCTATGACCATTTGTACGACTTTGGTCTTCATGACATCCTAACCGAGCTAATCGATGCTCGGAGAAGGGCCGGGATCCATTGCCGGAGCTCGGTGAAGATATACCACGCGAACAACGAAGGTTACGTTGCACAGATAGGTGATACACTGGTGATGAAGCTTGGACATTTCGATTGGAACCCGTCGAAGGAGAATCATCTGGAGGGGAGCTGGCAGACGTTTGTTGACAAAGGATCGGATTACAAACTGTGGGTACGACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

965

Amino Acids

109.0

Weight (kDa)

5.9

Isoelectric Point (pI)

48.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	596 - 660	9e-10	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	900 - 961	1.3e-15	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15550 AT5G15550 AT5G15550
fragaria_vesca	FvH4_6g46921 FvH4_6g46921 FvH4_6g46921 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930
malus_domestica	MD09G1066000.v1.1 MD09G1066100.v1.1 MD17G1058700.v1.1 MD17G1058800.v1.1
prunus_persica	Prupe.3G255400_v2.0.a1 Prupe.3G255500_v2.0.a1
pyrus_communis	pycom111g05550 pycom17g05870
rosa_chinensis	RchiOBHm_Chr2g0165901 RchiOBHm_Chr2g0165911
rosa_laevigata	RLG00000021569
rosa_multiflora	Rmu_co8422767.1_g000001 Rmu_sc0006218.1_g000010
rosa_roxburghii	Rroxscaffold_2G00085310 Rroxscaffold_2G00085320
rosa_rugosa	Rorug02G0519200 Rorug02G0519300
rosa_samantha	Rh2AG584700 Rh2AG584800 Rh2BG596600 Rh2CG567400 Rh2DG606700 Rh2DG606800
rosa_wichuraiana	Rw2G048750 Rw2G048760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	4 cut(s)	851, 2116, 2208, 2237
AccB7I	CCANNNNNTGG	2 cut(s)	1653, 1694
AccII	CGCG	2 cut(s)	2597, 2741
AclWI	GGATC	7 cut(s)	2057, 2378, 2459, 2551, 2701, 2714, 2872
AcoI	YGGCCR	1 cut(s)	2561
AcsI	RAATTY	6 cut(s)	989, 1428, 1507, 2068, 2141, 2323
AcyI	GRCGYC	2 cut(s)	88, 2209
AdeI	CACNNNGTG	1 cut(s)	1295
AfaI	GTAC	8 cut(s)	537, 1162, 1435, 1757, 1894, 2600, 2648, 2886
AflIII	ACRYGT	1 cut(s)	2595
AhlI	ACTAGT	1 cut(s)	167
AjiI	CACGTC	1 cut(s)	1706
AjnI	CCWGG	4 cut(s)	78, 1445, 1665, 1687
AjuI	GAANNNNNNNTTGG	6 cut(s)	272, 304, 428, 460, 902, 934
Alw21I	GWGCWC	4 cut(s)	1629, 1664, 1984, 2723
Alw26I	GTCTC	1 cut(s)	1237
AlwI	GGATC	7 cut(s)	2057, 2378, 2459, 2551, 2701, 2714, 2872
AlwNI	CAGNNNCTG	4 cut(s)	457, 635, 1694, 1835
Ama87I	CYCGRG	2 cut(s)	425, 1483
AoxI	GGCC	9 cut(s)	294, 573, 927, 1390, 2039, 2077, 2501, 2561, 2700
ApeKI	GCWGC	8 cut(s)	431, 457, 617, 632, 641, 1142, 1226, 1778
ApoI	RAATTY	6 cut(s)	989, 1428, 1507, 2068, 2141, 2323
ArsI	GACNNNNNNTTYG	2 cut(s)	2473, 2505
Asp700I	GAANNNNTTC	3 cut(s)	109, 131, 2089
AspLEI	GCGC	1 cut(s)	2211
AsuC2I	CCSGG	3 cut(s)	1400, 2616, 2704
AsuHPI	GGTGA	8 cut(s)	11, 685, 767, 1307, 2339, 2737, 2783, 2794
AsuII	TTCGAA	2 cut(s)	113, 2135
AvaI	CYCGRG	2 cut(s)	425, 1483
AvaII	GGWCC	3 cut(s)	44, 955, 1864
AxyI	CCTNAGG	1 cut(s)	2469
BaeI	ACNNNNGTAYC	3 cut(s)	2766, 2799, 2876
BamHI	GGATCC	1 cut(s)	2706
BanI	GGYRCC	4 cut(s)	851, 2116, 2208, 2237
BanII	GRGCYC	1 cut(s)	2723
BauI	CACGAG	2 cut(s)	2508, 2571
BbsI	GAAGAC	3 cut(s)	1080, 1649, 2651
Bbv12I	GWGCWC	4 cut(s)	1629, 1664, 1984, 2723
BbvI	GCAGC	8 cut(s)	443, 444, 619, 629, 653, 1129, 1213, 1765
BccI	CCATC	6 cut(s)	456, 1382, 1411, 2195, 2199, 2490
BceAI	ACGGC	4 cut(s)	839, 1821, 2017, 2548
BcgI	CGANNNNNNTGC	2 cut(s)	376, 410
BciT130I	CCWGG	4 cut(s)	80, 1447, 1667, 1689
BciVI	GTATCC	1 cut(s)	2585
BclI	TGATCA	1 cut(s)	2128
BcnI	CCSGG	3 cut(s)	1400, 2616, 2704
BcoDI	GTCTC	1 cut(s)	1237
BcuI	ACTAGT	1 cut(s)	167
BfaI	CTAG	4 cut(s)	168, 276, 303, 1422
BfmI	CTRYAG	3 cut(s)	627, 845, 1536
BfoI	RGCGCY	1 cut(s)	2212
BfuI	GTATCC	1 cut(s)	2585
BglI	GCCNNNNNGGC	1 cut(s)	148
BglII	AGATCT	2 cut(s)	1499, 1939
BlpI	GCTNAGC	1 cut(s)	2305
Bme1390I	CCNGG	7 cut(s)	80, 1400, 1447, 1667, 1689, 2616, 2704
Bme18I	GGWCC	3 cut(s)	44, 955, 1864
BmeT110I	CYCGRG	2 cut(s)	425, 1483
BmgBI	CACGTC	1 cut(s)	1706
BmrFI	CCNGG	7 cut(s)	80, 1400, 1447, 1667, 1689, 2616, 2704
BmrI	ACTGGG	1 cut(s)	1301
BmuI	ACTGGG	1 cut(s)	1301
BoxI	GACNNNNGTC	1 cut(s)	2655
BpiI	GAAGAC	3 cut(s)	1080, 1649, 2651
BpmI	CTGGAG	5 cut(s)	1004, 1643, 1762, 2505, 2852
Bpu10I	CCTNAGC	1 cut(s)	1542
Bpu1102I	GCTNAGC	1 cut(s)	2305
Bpu14I	TTCGAA	2 cut(s)	113, 2135
BpuEI	CTTGAG	3 cut(s)	1201, 1830, 1920
BpuMI	CCSGG	3 cut(s)	1400, 2616, 2704
Bsa29I	ATCGAT	2 cut(s)	1467, 2685
BsaHI	GRCGYC	2 cut(s)	88, 2209
BsaJI	CCNNGG	6 cut(s)	79, 360, 1445, 1665, 2080, 2394
BsaWI	WCCGGW	1 cut(s)	1700
BsaXI	ACNNNNNCTCC	2 cut(s)	1947, 1977
Bse118I	RCCGGY	1 cut(s)	2349
Bse1I	ACTGG	9 cut(s)	738, 810, 1002, 1296, 1699, 2180, 2488, 2564, 2784
Bse21I	CCTNAGG	1 cut(s)	2469
Bse3DI	GCAATG	4 cut(s)	30, 49, 980, 2710
BseBI	CCWGG	4 cut(s)	80, 1447, 1667, 1689
BseCI	ATCGAT	2 cut(s)	1467, 2685
BseDI	CCNNGG	6 cut(s)	79, 360, 1445, 1665, 2080, 2394
BseMI	GCAATG	4 cut(s)	30, 49, 980, 2710
BseMII	CTCAG	6 cut(s)	115, 887, 963, 1101, 1896, 2483
BseNI	ACTGG	9 cut(s)	738, 810, 1002, 1296, 1699, 2180, 2488, 2564, 2784
BseRI	GAGGAG	2 cut(s)	1043, 1833
BseXI	GCAGC	8 cut(s)	443, 444, 619, 629, 653, 1129, 1213, 1765
Bsh1236I	CGCG	2 cut(s)	2597, 2741
BshFI	GGCC	9 cut(s)	296, 575, 929, 1392, 2041, 2079, 2503, 2563, 2702
BshNI	GGYRCC	4 cut(s)	851, 2116, 2208, 2237
BshVI	ATCGAT	2 cut(s)	1467, 2685
BsiHKAI	GWGCWC	4 cut(s)	1629, 1664, 1984, 2723
BsiHKCI	CYCGRG	2 cut(s)	425, 1483
BsiSI	CCGG	6 cut(s)	1399, 1701, 2350, 2616, 2703, 2716
BslFI	GGGAC	2 cut(s)	1428, 2312
BsmAI	GTCTC	1 cut(s)	1237
BsmFI	GGGAC	2 cut(s)	1428, 2312
BsmI	GAATGC	1 cut(s)	1930
BsnI	GGCC	9 cut(s)	296, 575, 929, 1392, 2041, 2079, 2503, 2563, 2702
BsoBI	CYCGRG	2 cut(s)	425, 1483
Bsp119I	TTCGAA	2 cut(s)	113, 2135
Bsp1286I	GDGCHC	4 cut(s)	1629, 1664, 1984, 2723
Bsp1720I	GCTNAGC	1 cut(s)	2305
BspANI	GGCC	9 cut(s)	296, 575, 929, 1392, 2041, 2079, 2503, 2563, 2702
BspCNI	CTCAG	6 cut(s)	114, 886, 964, 1102, 1895, 2482
BspDI	ATCGAT	2 cut(s)	1467, 2685
BspFNI	CGCG	2 cut(s)	2597, 2741
BspHI	TCATGA	1 cut(s)	2662
BspPI	GGATC	7 cut(s)	2057, 2378, 2459, 2551, 2701, 2714, 2872
BspQI	GCTCTTC	1 cut(s)	2442
BspT104I	TTCGAA	2 cut(s)	113, 2135
BspT107I	GGYRCC	4 cut(s)	851, 2116, 2208, 2237
BsrDI	GCAATG	4 cut(s)	30, 49, 980, 2710
BsrFI	RCCGGY	1 cut(s)	2349
BsrI	ACTGG	9 cut(s)	738, 810, 1002, 1296, 1699, 2180, 2488, 2564, 2784
BssAI	RCCGGY	1 cut(s)	2349
BssECI	CCNNGG	6 cut(s)	79, 360, 1445, 1665, 2080, 2394
BssNI	GRCGYC	2 cut(s)	88, 2209
BssSI	CACGAG	2 cut(s)	2508, 2571
BssT1I	CCWWGG	1 cut(s)	2080
Bst2BI	CACGAG	2 cut(s)	2508, 2571
Bst2UI	CCWGG	4 cut(s)	80, 1447, 1667, 1689
Bst4CI	ACNGT	5 cut(s)	444, 708, 1897, 2880, 2892
Bst6I	CTCTTC	3 cut(s)	339, 468, 2442
BstACI	GRCGYC	2 cut(s)	88, 2209
BstAPI	GCANNNNNTGC	1 cut(s)	737
BstBI	TTCGAA	2 cut(s)	113, 2135
BstC8I	GCNNGC	5 cut(s)	573, 762, 816, 2031, 2843
BstFNI	CGCG	2 cut(s)	2597, 2741
BstH2I	RGCGCY	1 cut(s)	2212
BstHHI	GCGC	1 cut(s)	2211
BstMAI	GTCTC	1 cut(s)	1237
BstNI	CCWGG	4 cut(s)	80, 1447, 1667, 1689
BstNSI	RCATGY	5 cut(s)	357, 764, 941, 1161, 1583
BstPAI	GACNNNNGTC	1 cut(s)	2655
BstSCI	CCNGG	7 cut(s)	78, 1398, 1445, 1665, 1687, 2614, 2702
BstSFI	CTRYAG	3 cut(s)	627, 845, 1536
BstUI	CGCG	2 cut(s)	2597, 2741
BstV1I	GCAGC	8 cut(s)	443, 444, 619, 629, 653, 1129, 1213, 1765
BstV2I	GAAGAC	3 cut(s)	1080, 1649, 2651
BstX2I	RGATCY	4 cut(s)	1499, 1939, 2464, 2706
BstXI	CCANNNNNNTGG	1 cut(s)	1153
BstYI	RGATCY	4 cut(s)	1499, 1939, 2464, 2706
Bsu15I	ATCGAT	2 cut(s)	1467, 2685
Bsu36I	CCTNAGG	1 cut(s)	2469
BsuI	GTATCC	1 cut(s)	2585
BsuRI	GGCC	9 cut(s)	296, 575, 929, 1392, 2041, 2079, 2503, 2563, 2702
BsuTUI	ATCGAT	2 cut(s)	1467, 2685
BtgZI	GCGATG	1 cut(s)	1401
BtrI	CACGTC	1 cut(s)	1706
BtsI	GCAGTG	5 cut(s)	22, 1196, 1757, 1815, 1912
Cac8I	GCNNGC	5 cut(s)	573, 762, 816, 2031, 2843
CaiI	CAGNNNCTG	4 cut(s)	457, 635, 1694, 1835
CciI	TCATGA	1 cut(s)	2662
CfoI	GCGC	1 cut(s)	2211
Cfr10I	RCCGGY	1 cut(s)	2349
ClaI	ATCGAT	2 cut(s)	1467, 2685
CseI	GACGC	1 cut(s)	77
Csp6I	GTAC	8 cut(s)	536, 1161, 1434, 1756, 1893, 2599, 2647, 2885
CviAII	CATG	9 cut(s)	184, 354, 761, 938, 1158, 1580, 2111, 2318, 2663
CviQI	GTAC	8 cut(s)	536, 1161, 1434, 1756, 1893, 2599, 2647, 2885
DinI	GGCGCC	1 cut(s)	2210
DraIII	CACNNNGTG	1 cut(s)	1295
EaeI	YGGCCR	1 cut(s)	2561
Eam1104I	CTCTTC	3 cut(s)	339, 468, 2442
EarI	CTCTTC	3 cut(s)	339, 468, 2442
EciI	GGCGGA	2 cut(s)	758, 1351
Ecl136II	GAGCTC	1 cut(s)	2721
Eco130I	CCWWGG	1 cut(s)	2080
Eco24I	GRGCYC	1 cut(s)	2723
Eco47I	GGWCC	3 cut(s)	44, 955, 1864
Eco53kI	GAGCTC	1 cut(s)	2721
Eco81I	CCTNAGG	1 cut(s)	2469
Eco88I	CYCGRG	2 cut(s)	425, 1483
EcoICRI	GAGCTC	1 cut(s)	2721
EcoO109I	RGGNCCY	2 cut(s)	928, 2039
EcoRII	CCWGG	4 cut(s)	78, 1445, 1665, 1687
EcoT14I	CCWWGG	1 cut(s)	2080
EcoT22I	ATGCAT	3 cut(s)	766, 1177, 1932
EcoT38I	GRGCYC	1 cut(s)	2723
EgeI	GGCGCC	1 cut(s)	2210
EheI	GGCGCC	1 cut(s)	2210
ErhI	CCWWGG	1 cut(s)	2080
FaeI	CATG	9 cut(s)	187, 357, 764, 941, 1161, 1583, 2114, 2321, 2666
FalI	AAGNNNNNCTT	4 cut(s)	470, 502, 969, 1001
FaqI	GGGAC	2 cut(s)	1428, 2312
FatI	CATG	9 cut(s)	183, 353, 760, 937, 1157, 1579, 2110, 2317, 2662
FauNDI	CATATG	1 cut(s)	1888
FbaI	TGATCA	1 cut(s)	2128
FriOI	GRGCYC	1 cut(s)	2723
FspBI	CTAG	4 cut(s)	168, 276, 303, 1422
GlaI	GCGC	1 cut(s)	2210
GsuI	CTGGAG	5 cut(s)	1004, 1643, 1762, 2505, 2852
HaeII	RGCGCY	1 cut(s)	2212
HaeIII	GGCC	9 cut(s)	296, 575, 929, 1392, 2041, 2079, 2503, 2563, 2702
HapII	CCGG	6 cut(s)	1399, 1701, 2350, 2616, 2703, 2716
HgaI	GACGC	1 cut(s)	77
HhaI	GCGC	1 cut(s)	2211
Hin1I	GRCGYC	2 cut(s)	88, 2209
Hin1II	CATG	9 cut(s)	187, 357, 764, 941, 1161, 1583, 2114, 2321, 2666
Hin6I	GCGC	1 cut(s)	2209
HinP1I	GCGC	1 cut(s)	2209
HincII	GTYRAC	1 cut(s)	2857
HindII	GTYRAC	1 cut(s)	2857
HindIII	AAGCTT	4 cut(s)	2027, 2262, 2579, 2789
HpaII	CCGG	6 cut(s)	1399, 1701, 2350, 2616, 2703, 2716
HphI	GGTGA	8 cut(s)	11, 685, 767, 1307, 2339, 2737, 2783, 2794
Hpy166II	GTNNAC	4 cut(s)	47, 803, 2477, 2857
Hpy188III	TCNNGA	8 cut(s)	162, 995, 1021, 1622, 1937, 2339, 2663, 2831
Hpy8I	GTNNAC	4 cut(s)	47, 803, 2477, 2857
Hpy99I	CGWCG	1 cut(s)	2818
HpyCH4III	ACNGT	5 cut(s)	444, 708, 1897, 2880, 2892
HpyCH4IV	ACGT	8 cut(s)	213, 897, 1070, 1629, 1705, 2519, 2757, 2849
HpySE526I	ACGT	8 cut(s)	213, 897, 1070, 1629, 1705, 2519, 2757, 2849
Hsp92I	GRCGYC	2 cut(s)	88, 2209
Hsp92II	CATG	9 cut(s)	187, 357, 764, 941, 1161, 1583, 2114, 2321, 2666
HspAI	GCGC	1 cut(s)	2209
KasI	GGCGCC	1 cut(s)	2208
Ksp22I	TGATCA	1 cut(s)	2128
LguI	GCTCTTC	1 cut(s)	2442
LmnI	GCTCC	8 cut(s)	311, 1139, 1624, 1774, 1821, 1846, 2718, 2838
Lsp1109I	GCAGC	8 cut(s)	443, 444, 619, 629, 653, 1129, 1213, 1765
MaeI	CTAG	4 cut(s)	168, 276, 303, 1422
MaeII	ACGT	8 cut(s)	213, 897, 1070, 1629, 1705, 2519, 2757, 2849
MaeIII	GTNAC	9 cut(s)	438, 673, 893, 1801, 2330, 2407, 2515, 2555, 2753
MfeI	CAATTG	4 cut(s)	335, 1729, 2049, 2365
MflI	RGATCY	4 cut(s)	1499, 1939, 2464, 2706
MhlI	GDGCHC	4 cut(s)	1629, 1664, 1984, 2723
MluI	ACGCGT	1 cut(s)	2595
Mly113I	GGCGCC	1 cut(s)	2209
MlyI	GAGTC	3 cut(s)	500, 808, 2002
MmeI	TCCRAC	2 cut(s)	894, 900
Mph1103I	ATGCAT	3 cut(s)	766, 1177, 1932
MroXI	GAANNNNTTC	3 cut(s)	109, 131, 2089
MseI	TTAA	5 cut(s)	666, 695, 1326, 1382, 1908
MslI	CAYNNNNRTG	4 cut(s)	942, 1369, 1584, 1977
MspA1I	CMGCKG	1 cut(s)	632
MspI	CCGG	6 cut(s)	1399, 1701, 2350, 2616, 2703, 2716
MspR9I	CCNGG	7 cut(s)	80, 1400, 1447, 1667, 1689, 2616, 2704
MunI	CAATTG	4 cut(s)	335, 1729, 2049, 2365
Mva1269I	GAATGC	1 cut(s)	1930
MvaI	CCWGG	4 cut(s)	80, 1447, 1667, 1689
MvnI	CGCG	2 cut(s)	2597, 2741
NarI	GGCGCC	1 cut(s)	2209
NciI	CCSGG	3 cut(s)	1400, 2616, 2704
NdeI	CATATG	1 cut(s)	1888
NlaIII	CATG	9 cut(s)	187, 357, 764, 941, 1161, 1583, 2114, 2321, 2666
NmeAIII	GCCGAG	1 cut(s)	2375
NmuCI	GTSAC	6 cut(s)	438, 673, 893, 2407, 2515, 2555
NsiI	ATGCAT	3 cut(s)	766, 1177, 1932
NspI	RCATGY	5 cut(s)	357, 764, 941, 1161, 1583
NspV	TTCGAA	2 cut(s)	113, 2135
PaeI	GCATGC	1 cut(s)	764
PaeR7I	CTCGAG	2 cut(s)	425, 1483
PagI	TCATGA	1 cut(s)	2662
PciSI	GCTCTTC	1 cut(s)	2442
PcsI	WCGNNNNNNNCGW	3 cut(s)	423, 2745, 2754
PctI	GAATGC	1 cut(s)	1930
PdmI	GAANNNNTTC	3 cut(s)	109, 131, 2089
PfeI	GAWTC	9 cut(s)	191, 464, 869, 998, 1265, 1310, 1361, 1832, 2824
PflMI	CCANNNNNTGG	2 cut(s)	1653, 1694
PfoI	TCCNGGA	1 cut(s)	2614
PleI	GAGTC	3 cut(s)	499, 807, 2001
PluTI	GGCGCC	1 cut(s)	2212
PpsI	GAGTC	3 cut(s)	499, 807, 2001
PshAI	GACNNNNGTC	1 cut(s)	2655
Psp124BI	GAGCTC	1 cut(s)	2723
Psp6I	CCWGG	4 cut(s)	78, 1445, 1665, 1687
PspGI	CCWGG	4 cut(s)	78, 1445, 1665, 1687
PspXI	VCTCGAGB	2 cut(s)	425, 1483
PsrI	GAACNNNNNNTAC	4 cut(s)	119, 151, 519, 551
PstNI	CAGNNNCTG	4 cut(s)	457, 635, 1694, 1835
PsuI	RGATCY	4 cut(s)	1499, 1939, 2464, 2706
PvuII	CAGCTG	1 cut(s)	632
RsaI	GTAC	8 cut(s)	537, 1162, 1435, 1757, 1894, 2600, 2648, 2886
RsaNI	GTAC	8 cut(s)	536, 1161, 1434, 1756, 1893, 2599, 2647, 2885
RseI	CAYNNNNRTG	4 cut(s)	942, 1369, 1584, 1977
SacI	GAGCTC	1 cut(s)	2723
SapI	GCTCTTC	1 cut(s)	2442
SaqAI	TTAA	5 cut(s)	666, 695, 1326, 1382, 1908
SchI	GAGTC	3 cut(s)	500, 808, 2002
ScrFI	CCNGG	7 cut(s)	80, 1400, 1447, 1667, 1689, 2616, 2704
SduI	GDGCHC	4 cut(s)	1629, 1664, 1984, 2723
SfcI	CTRYAG	3 cut(s)	627, 845, 1536
SfoI	GGCGCC	1 cut(s)	2210
Sfr274I	CTCGAG	2 cut(s)	425, 1483
SfuI	TTCGAA	2 cut(s)	113, 2135
SinI	GGWCC	3 cut(s)	44, 955, 1864
SlaI	CTCGAG	2 cut(s)	425, 1483
SmiMI	CAYNNNNRTG	4 cut(s)	942, 1369, 1584, 1977
SmlI	CTYRAG	5 cut(s)	425, 1180, 1483, 1845, 1935
SmoI	CTYRAG	5 cut(s)	425, 1180, 1483, 1845, 1935
SpeI	ACTAGT	1 cut(s)	167
SphI	GCATGC	1 cut(s)	764
SspDI	GGCGCC	1 cut(s)	2208
SspI	AATATT	4 cut(s)	838, 2125, 2294, 2450
SspMI	CTAG	4 cut(s)	168, 276, 303, 1422
SstI	GAGCTC	1 cut(s)	2723
StyD4I	CCNGG	7 cut(s)	78, 1398, 1445, 1665, 1687, 2614, 2702
StyI	CCWWGG	1 cut(s)	2080
TaaI	ACNGT	5 cut(s)	444, 708, 1897, 2880, 2892
TaiI	ACGT	8 cut(s)	216, 900, 1073, 1632, 1708, 2522, 2760, 2852
TaqI	TCGA	8 cut(s)	113, 426, 1467, 1484, 2135, 2685, 2802, 2816
TauI	GCSGC	5 cut(s)	78, 88, 743, 2117, 2388
TfiI	GAWTC	9 cut(s)	191, 464, 869, 998, 1265, 1310, 1361, 1832, 2824
Tru1I	TTAA	5 cut(s)	666, 695, 1326, 1382, 1908
Tru9I	TTAA	5 cut(s)	666, 695, 1326, 1382, 1908
TseFI	GTSAC	6 cut(s)	438, 673, 893, 2407, 2515, 2555
TseI	GCWGC	8 cut(s)	431, 457, 617, 632, 641, 1142, 1226, 1778
Tsp45I	GTSAC	6 cut(s)	438, 673, 893, 2407, 2515, 2555
TspGWI	ACGGA	1 cut(s)	2371
Van91I	CCANNNNNTGG	2 cut(s)	1653, 1694
VpaK11BI	GGWCC	3 cut(s)	44, 955, 1864
XapI	RAATTY	6 cut(s)	989, 1428, 1507, 2068, 2141, 2323
XceI	RCATGY	5 cut(s)	357, 764, 941, 1161, 1583
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2004
XhoI	CTCGAG	2 cut(s)	425, 1483
XmnI	GAANNNNTTC	3 cut(s)	109, 131, 2089
XspI	CTAG	4 cut(s)	168, 276, 303, 1422
Zsp2I	ATGCAT	3 cut(s)	766, 1177, 1932

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.