Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003013

Physical Location & Seq

Genomic Coordinates Forward (+)

76319466 .. 76327318

Gene Structure 7853 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G048760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGGGTGTTATATCATTGCCTGATATGGCAGCTGGGGTTATTCCCAACCTCGCCGTGTCATATTCAGGTGGACATTGCAAACATGATAGGACTTTATTCCTGCGGCGGCGCCCTCTTGTTTTCAGAACAAACTCGAATAGAAAAAAGAAACTATCCCTCACAGAACATTGGCTATGTAAATCAAGAACAAGCATATTTTCAAGCCTGGATGATTCCAGTGTTACAGATGTTGTTGACAACGGTGACAGTTTTTCTTCAGGAAGAAATGAAATGTTGAGTGTAGAGGAGGATGAGTTAATGACAGCTAAATTAGCACTTTCTGAGGCTCATGCTAGACAAGAAGCCATTGAGAAAGAGAGGGATCAATTGCTTGAAGAGTTGGCATGTTTTGAGGCAAAACAACAGGAATATGTAGCTACCATATTGCACGACAAGGATGTGGCTGTTTCGGAACTTGAGGCTGCAAAATCTTTGTTCAATCAGAAGCTGCAGGAATCAGTTCAAGAGAAGTTCAGCTTGCAAGATAAGTTAGTCCTTGTGAAACAAGATGCTGTTCAACTTGCGGTACAAGTAGAGAGGCTAGCAGAAATTGCTTTTCAGCAGGCAACATCTCACATACTAGAAGATGCCCAGTTGAGGGTTTCCGCTGCTGAAACTACAGCTGCTGAAGCATCTTATCGGATAGAAAAGCAAATTAAGGATATGACTGAAGGTACAATATTGTCGATTGTGGAACAGTCGAAAAATGCTATAGAGAAGGCCCTGGACGTGGCAGAAAAAGCTGGTGACCATGCATCAAAAGCTGCGTCAGCATTTAATGACAGTATGAGTCCACTTGATGAGCTTGCTTCGGTCCAGTCAAAGAACATCATGCTACAGGGTGCTGTGAATGATTTAGAATCTCAGTTATTGCTTACAAAAAGTGATGTCGCTAAGTTGAAGTTGGAGTTGGAAAAGGCCCATGCATATGCAAATTTGCTTGAGGTCCGAGCTACTGATGCTGAGAAAGCATTGGTTGAATTTCAGGAGTCAAGCAGCAAAGAGAGTCTCCAGAGAGAGCAGGAAATCATGTCATTGATGGAGCAGATGAAGAAAGATTCATCGGAAAGAAACCAGGCTGCTTCAGGGGCTTTCAATGTTGAGTTACAAAGCATTAGGGATGCTGTTGCAGCTGTGAAAGAAACAGTACATTCCAAAGATGATGCCTACTTGAGAAGATGCGAAGCACTGCAAAGATCGTTGAAGGCATCTGAAGCTACTACAAAGATGTGGAGACAGAGAGCAGAAACTGCAGAATCCTTGTTATTGAAGGAAAGGCAGCCAGGTGATTGGGAAGAAGATTCCATTTTTGTTGTTAATGGTGGAAGGATAGACCTTTTGACCAATGATGATTCACAGAAATGGAAGCTTTTAAGTGATGGTCCTCGCAGAGAGATACCTCAATGGATGGCAAGGAGAATATGTACCATCCGTACCAAGTTTCCTCCAAGGAAGATTGATGTAGCTGAAGCCTTGAGTTCAGAGTTCAGATCTTTGAACTTGCCAAAGCCTGAGGAAGTATGGTCAATAGCTCTGGAAAAGCCAAAGGACGGGGATACACTTATTGAGCATGCTTTTGAGAAAGAAACAATAGAGAAGAAAAGGAAGGCTCTTGAGCGTGTTCTTCAGCGGAAGACCACACAATGGCAGAGGACTGAGGAACAAACAAAATTAGAGCCAGGAACTGGAACAGGACGTGAGATTGTGTTTCAAGGTTTCAATTGGGAAAGCTGGAGAAAGCAGTGGTACCTTGATTTAGCTCCTAAAGCTTCTGATTTATCTAAAATTGGGGTAACATCAGTGTGGTTCCCACCACCAACGGAATCCGTTGCTCCTCAAGGTTATATGCCTTCTGACCTTTACAATTTGAACTCGGCATATGGTACTAAGGAAGAACTTCAACACTGCATTGCGGAGATGCATGCTCATGATCTTCTGGCCTTGGGAGATGTTGTCCTGAATCATCGATGTGCTCATAAACAGAGTCCAAATGGTGTTTGGAACATCTTTGGTGGGAAGCTTGCATGGGGGCCTGAAGCAATCGTTTGTGATGATCCAAATTTTGAGGGCCGTGGAAACCCTTCGAGTGGGGATATATTCCATGCAGCACCCAATATTGATCATTCAAAGGACTTTGTAAGAAAAGATATAAAGGAGTGGCTAAACTGGCTTAGGAGTGATATTGGTTTTGATGGATGGCGCCTTGATTTTGTAAGAGGCTTCTCTGGTACCCATGTAAAGGAATATATTGAAGCTTCGAATCCAGCATTCGCTATTGGAGAATATTGGGACAGTTTAGCTTATGAAAATGGAAGTTTGTGTTACAATCAAGATGCTCATCGTCAAAGGATAGTTAATTGGATCAATGCTACAGGCGGCAGTTCCTCAGCATTTGATGTCACAACTAAGGGAATTCTCCATTCTGCTCTTCATAACCAATATTGGAGGTTAATAGATCCTCAGGGTAAACCAACTGGAGTTATGGGATGGTGGCCCTCACGTGCTGTTACATTTTTGGAGAACCATGACACAGGATCAACGCAGGGTCATTGGCCATTTCCACGAGAAAAGCTTACACAGGGATATGCATACATTTTGACCCATCCTGGAACACCTACCATCTTTTACGACCATTTATACGACTTTGGTCTTCACGAGATCCTAAGTGAGCTCATTGAGGCTCGGAGAAGGGCAGGGATCCATTGCCGGAGCGCCGTGAAGATATACCATGCAAACAATGAAGGGTATGTTGCACATATAGGTGACACTCTAGTAATGAAGCTCGGACATTTCGACTGGAATCCCTCAAAGGAAAACCATTTGGAAGGGAGCTGGCAGAAGTTTGTTGATAAAGGAGCAGATTACATAGTGTGGTTACGACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

974

Amino Acids

110.05

Weight (kDa)

5.7

Isoelectric Point (pI)

48.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	606 - 670	9.5e-09	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	910 - 971	6.2e-16	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15550 AT5G15550 AT5G15550
fragaria_vesca	FvH4_6g46921 FvH4_6g46921 FvH4_6g46921 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930
malus_domestica	MD09G1066000.v1.1 MD09G1066100.v1.1 MD17G1058700.v1.1 MD17G1058800.v1.1
prunus_persica	Prupe.3G255400_v2.0.a1 Prupe.3G255500_v2.0.a1
pyrus_communis	pycom111g05550 pycom17g05870
rosa_chinensis	RchiOBHm_Chr2g0165901 RchiOBHm_Chr2g0165911
rosa_laevigata	RLG00000021569
rosa_multiflora	Rmu_co8422767.1_g000001 Rmu_sc0006218.1_g000010
rosa_roxburghii	Rroxscaffold_2G00085310 Rroxscaffold_2G00085320
rosa_rugosa	Rorug02G0519200 Rorug02G0519300
rosa_samantha	Rh2AG584700 Rh2AG584800 Rh2BG596600 Rh2CG567400 Rh2DG606700 Rh2DG606800
rosa_wichuraiana	Rw2G048750 Rw2G048760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	1785, 2267
AccB1I	GGYRCC	4 cut(s)	108, 1785, 2238, 2267
AccB7I	CCANNNNNTGG	2 cut(s)	1683, 1724
AciI	CCGC	7 cut(s)	103, 106, 563, 645, 1669, 1952, 2415
AclWI	GGATC	8 cut(s)	369, 2087, 2408, 2489, 2581, 2692, 2731, 2744
AcoI	YGGCCR	1 cut(s)	2591
AcsI	RAATTY	4 cut(s)	973, 1019, 2098, 2451
AcuI	CTGAAG	8 cut(s)	240, 687, 729, 1107, 1272, 1527, 1649, 2094
AcvI	CACGTG	1 cut(s)	2540
AcyI	GRCGYC	2 cut(s)	109, 2239
AfaI	GTAC	8 cut(s)	567, 715, 1188, 1465, 1474, 1787, 1924, 2269
AfiI	CCNNNNNNNGG	8 cut(s)	636, 637, 769, 1589, 1683, 1724, 2126, 2278
AjiI	CACGTC	2 cut(s)	769, 1736
AjnI	CCWGG	6 cut(s)	204, 762, 1113, 1321, 1717, 2644
AjuI	GAANNNNNNNTTGG	2 cut(s)	932, 964
Alw21I	GWGCWC	2 cut(s)	2014, 2712
Alw26I	GTCTC	2 cut(s)	1052, 1267
AlwI	GGATC	8 cut(s)	369, 2087, 2408, 2489, 2581, 2692, 2731, 2744
AlwNI	CAGNNNCTG	4 cut(s)	487, 665, 1289, 1724
AoxI	GGCC	7 cut(s)	759, 957, 1977, 2069, 2107, 2531, 2591
ApoI	RAATTY	4 cut(s)	973, 1019, 2098, 2451
ArsI	GACNNNNNNTTYG	2 cut(s)	791, 823
Asp700I	GAANNNNTTC	3 cut(s)	498, 1935, 2119
Asp718I	GGTACC	2 cut(s)	1785, 2267
AspLEI	GCGC	3 cut(s)	111, 2241, 2753
AspS9I	GGNCC	8 cut(s)	760, 853, 958, 985, 1421, 2069, 2107, 2532
AsuHPI	GGTGA	4 cut(s)	254, 797, 1337, 2813
AsuII	TTCGAA	1 cut(s)	2297
AsuNHI	GCTAGC	1 cut(s)	580
AvaII	GGWCC	3 cut(s)	853, 985, 1421
AxyI	CCTNAGG	2 cut(s)	1551, 2499
BaeI	ACNNNNGTAYC	2 cut(s)	2259, 2292
BalI	TGGCCA	1 cut(s)	2593
BamHI	GGATCC	1 cut(s)	2736
BanI	GGYRCC	4 cut(s)	108, 1785, 2238, 2267
BanII	GRGCYC	1 cut(s)	2712
BauI	CACGAG	2 cut(s)	2601, 2693
BbrPI	CACGTG	1 cut(s)	2540
BbsI	GAAGAC	2 cut(s)	1679, 2681
Bbv12I	GWGCWC	2 cut(s)	2014, 2712
BbvCI	CCTCAGC	1 cut(s)	2425
BccI	CCATC	9 cut(s)	1072, 1412, 1441, 1475, 2225, 2229, 2520, 2649, 2666
BceAI	ACGGC	3 cut(s)	38, 2094, 2738
BciT130I	CCWGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
BciVI	GTATCC	1 cut(s)	1588
BclI	TGATCA	1 cut(s)	2158
BcoDI	GTCTC	2 cut(s)	1052, 1267
BfaI	CTAG	4 cut(s)	333, 581, 620, 2810
BfmI	CTRYAG	6 cut(s)	488, 657, 750, 875, 1290, 2409
BfoI	RGCGCY	3 cut(s)	112, 2242, 2754
BfuI	GTATCC	1 cut(s)	1588
BglII	AGATCT	1 cut(s)	1529
Bme1390I	CCNGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
Bme18I	GGWCC	3 cut(s)	853, 985, 1421
BmgBI	CACGTC	2 cut(s)	769, 1736
BmgT120I	GGNCC	8 cut(s)	760, 853, 958, 985, 1421, 2069, 2107, 2532
BmiI	GGNNCC	7 cut(s)	110, 1787, 1847, 2070, 2240, 2269, 2738
BmrFI	CCNGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
BmrI	ACTGGG	1 cut(s)	625
BmtI	GCTAGC	1 cut(s)	584
BmuI	ACTGGG	1 cut(s)	625
BoxI	GACNNNNGTC	1 cut(s)	2685
BpiI	GAAGAC	2 cut(s)	1679, 2681
BpmI	CTGGAG	3 cut(s)	1034, 1792, 2535
Bpu10I	CCTNAGC	2 cut(s)	2210, 2425
Bpu14I	TTCGAA	1 cut(s)	2297
BpuEI	CTTGAG	6 cut(s)	476, 1001, 1231, 1534, 1673, 1860
Bsa29I	ATCGAT	1 cut(s)	2005
BsaAI	YACGTR	1 cut(s)	2540
BsaBI	GATNNNNATC	1 cut(s)	2376
BsaHI	GRCGYC	2 cut(s)	109, 2239
BsaJI	CCNNGG	4 cut(s)	762, 1487, 1980, 2110
BsaXI	ACNNNNNCTCC	6 cut(s)	278, 308, 1447, 1477, 1977, 2007
Bsc4I	CCNNNNNNNGG	8 cut(s)	636, 637, 769, 1589, 1683, 1724, 2126, 2278
Bse1I	ACTGG	7 cut(s)	216, 631, 856, 1729, 2210, 2518, 2840
Bse21I	CCTNAGG	2 cut(s)	1551, 2499
Bse3DI	GCAATG	4 cut(s)	14, 73, 1947, 2740
Bse8I	GATNNNNATC	1 cut(s)	2376
BseBI	CCWGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
BseCI	ATCGAT	1 cut(s)	2005
BseDI	CCNNGG	4 cut(s)	762, 1487, 1980, 2110
BseGI	GGATG	9 cut(s)	214, 295, 442, 1165, 1452, 1467, 2240, 2531, 2641
BseJI	GATNNNNATC	1 cut(s)	2376
BseLI	CCNNNNNNNGG	8 cut(s)	636, 637, 769, 1589, 1683, 1724, 2126, 2278
BseMI	GCAATG	4 cut(s)	14, 73, 1947, 2740
BseMII	CTCAG	7 cut(s)	312, 917, 993, 1542, 1686, 2439, 2513
BseNI	ACTGG	7 cut(s)	216, 631, 856, 1729, 2210, 2518, 2840
BseRI	GAGGAG	2 cut(s)	299, 1863
BseYI	CCCAGC	1 cut(s)	32
BshFI	GGCC	7 cut(s)	761, 959, 1979, 2071, 2109, 2533, 2593
BshNI	GGYRCC	4 cut(s)	108, 1785, 2238, 2267
BshVI	ATCGAT	1 cut(s)	2005
BsiHKAI	GWGCWC	2 cut(s)	2014, 2712
BsiSI	CCGG	1 cut(s)	2746
BslFI	GGGAC	1 cut(s)	2342
BslI	CCNNNNNNNGG	8 cut(s)	636, 637, 769, 1589, 1683, 1724, 2126, 2278
BsmAI	GTCTC	2 cut(s)	1052, 1267
BsmFI	GGGAC	1 cut(s)	2342
BsmI	GAATGC	1 cut(s)	2306
BsnI	GGCC	7 cut(s)	761, 959, 1979, 2071, 2109, 2533, 2593
Bsp119I	TTCGAA	1 cut(s)	2297
Bsp1286I	GDGCHC	2 cut(s)	2014, 2712
BspACI	CCGC	7 cut(s)	103, 106, 563, 645, 1669, 1952, 2415
BspANI	GGCC	7 cut(s)	761, 959, 1979, 2071, 2109, 2533, 2593
BspCNI	CTCAG	7 cut(s)	313, 916, 994, 1543, 1687, 2438, 2512
BspDI	ATCGAT	1 cut(s)	2005
BspHI	TCATGA	1 cut(s)	1966
BspLI	GGNNCC	7 cut(s)	110, 1787, 1847, 2070, 2240, 2269, 2738
BspMAI	CTGCAG	2 cut(s)	492, 1294
BspOI	GCTAGC	1 cut(s)	584
BspPI	GGATC	8 cut(s)	369, 2087, 2408, 2489, 2581, 2692, 2731, 2744
BspQI	GCTCTTC	1 cut(s)	2472
BspT104I	TTCGAA	1 cut(s)	2297
BspT107I	GGYRCC	4 cut(s)	108, 1785, 2238, 2267
BsrDI	GCAATG	4 cut(s)	14, 73, 1947, 2740
BsrI	ACTGG	7 cut(s)	216, 631, 856, 1729, 2210, 2518, 2840
BssECI	CCNNGG	4 cut(s)	762, 1487, 1980, 2110
BssNI	GRCGYC	2 cut(s)	109, 2239
BssSI	CACGAG	2 cut(s)	2601, 2693
BssT1I	CCWWGG	2 cut(s)	1487, 1980
Bst2BI	CACGAG	2 cut(s)	2601, 2693
Bst2UI	CCWGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
Bst4CI	ACNGT	6 cut(s)	242, 248, 738, 824, 1186, 2333
Bst6I	CTCTTC	2 cut(s)	369, 2472
BstACI	GRCGYC	2 cut(s)	109, 2239
BstAPI	GCANNNNNTGC	2 cut(s)	590, 1289
BstBAI	YACGTR	1 cut(s)	2540
BstBI	TTCGAA	1 cut(s)	2297
BstC8I	GCNNGC	8 cut(s)	518, 582, 603, 846, 1611, 1962, 2061, 2873
BstDSI	CCRYGG	1 cut(s)	2110
BstEII	GGTNACC	1 cut(s)	785
BstF5I	GGATG	9 cut(s)	214, 295, 442, 1165, 1452, 1467, 2240, 2531, 2641
BstH2I	RGCGCY	3 cut(s)	112, 2242, 2754
BstHHI	GCGC	3 cut(s)	111, 2241, 2753
BstMAI	GTCTC	2 cut(s)	1052, 1267
BstNI	CCWGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
BstNSI	RCATGY	3 cut(s)	387, 1613, 1964
BstPAI	GACNNNNGTC	1 cut(s)	2685
BstPI	GGTNACC	1 cut(s)	785
BstSCI	CCNGG	6 cut(s)	204, 762, 1113, 1321, 1717, 2644
BstSFI	CTRYAG	6 cut(s)	488, 657, 750, 875, 1290, 2409
BstV2I	GAAGAC	2 cut(s)	1679, 2681
BstX2I	RGATCY	4 cut(s)	1529, 2494, 2697, 2736
BstXI	CCANNNNNNTGG	1 cut(s)	1329
BstYI	RGATCY	4 cut(s)	1529, 2494, 2697, 2736
Bsu15I	ATCGAT	1 cut(s)	2005
Bsu36I	CCTNAGG	2 cut(s)	1551, 2499
BsuI	GTATCC	1 cut(s)	1588
BsuRI	GGCC	7 cut(s)	761, 959, 1979, 2071, 2109, 2533, 2593
BsuTUI	ATCGAT	1 cut(s)	2005
BtgI	CCRYGG	1 cut(s)	2110
BtrI	CACGTC	2 cut(s)	769, 1736
BtsCI	GGATG	9 cut(s)	214, 295, 442, 1165, 1452, 1467, 2240, 2531, 2641
BtsI	GCAGTG	3 cut(s)	1226, 1787, 1942
BtsIMutI	CAGTG	5 cut(s)	223, 1226, 1787, 1845, 1942
Cac8I	GCNNGC	8 cut(s)	518, 582, 603, 846, 1611, 1962, 2061, 2873
CaiI	CAGNNNCTG	4 cut(s)	487, 665, 1289, 1724
CciI	TCATGA	1 cut(s)	1966
CfoI	GCGC	3 cut(s)	111, 2241, 2753
Cfr13I	GGNCC	8 cut(s)	760, 853, 958, 985, 1421, 2069, 2107, 2532
ClaI	ATCGAT	1 cut(s)	2005
CseI	GACGC	1 cut(s)	795
Csp6I	GTAC	8 cut(s)	566, 714, 1187, 1464, 1473, 1786, 1923, 2268
CviQI	GTAC	8 cut(s)	566, 714, 1187, 1464, 1473, 1786, 1923, 2268
DinI	GGCGCC	2 cut(s)	110, 2240
EaeI	YGGCCR	1 cut(s)	2591
Eam1104I	CTCTTC	2 cut(s)	369, 2472
EarI	CTCTTC	2 cut(s)	369, 2472
Ecl136II	GAGCTC	1 cut(s)	2710
Eco130I	CCWWGG	2 cut(s)	1487, 1980
Eco24I	GRGCYC	1 cut(s)	2712
Eco47I	GGWCC	3 cut(s)	853, 985, 1421
Eco53kI	GAGCTC	1 cut(s)	2710
Eco57I	CTGAAG	8 cut(s)	240, 687, 729, 1107, 1272, 1527, 1649, 2094
Eco72I	CACGTG	1 cut(s)	2540
Eco81I	CCTNAGG	2 cut(s)	1551, 2499
Eco91I	GGTNACC	1 cut(s)	785
EcoICRI	GAGCTC	1 cut(s)	2710
EcoO109I	RGGNCCY	2 cut(s)	760, 2069
EcoO65I	GGTNACC	1 cut(s)	785
EcoRI	GAATTC	1 cut(s)	2451
EcoRII	CCWGG	6 cut(s)	204, 762, 1113, 1321, 1717, 2644
EcoT14I	CCWWGG	2 cut(s)	1487, 1980
EcoT22I	ATGCAT	4 cut(s)	796, 967, 1962, 2629
EcoT38I	GRGCYC	1 cut(s)	2712
EgeI	GGCGCC	2 cut(s)	110, 2240
EheI	GGCGCC	2 cut(s)	110, 2240
ErhI	CCWWGG	2 cut(s)	1487, 1980
FalI	AAGNNNNNCTT	2 cut(s)	500, 532
FaqI	GGGAC	1 cut(s)	2342
FauNDI	CATATG	2 cut(s)	967, 1918
FbaI	TGATCA	1 cut(s)	2158
FokI	GGATG	9 cut(s)	221, 302, 449, 1172, 1454, 1459, 2247, 2538, 2628
FriOI	GRGCYC	1 cut(s)	2712
FspBI	CTAG	4 cut(s)	333, 581, 620, 2810
GlaI	GCGC	3 cut(s)	110, 2240, 2752
GsaI	CCCAGC	1 cut(s)	36
GsuI	CTGGAG	3 cut(s)	1034, 1792, 2535
HaeII	RGCGCY	3 cut(s)	112, 2242, 2754
HaeIII	GGCC	7 cut(s)	761, 959, 1979, 2071, 2109, 2533, 2593
HapII	CCGG	1 cut(s)	2746
HgaI	GACGC	1 cut(s)	795
HhaI	GCGC	3 cut(s)	111, 2241, 2753
Hin1I	GRCGYC	2 cut(s)	109, 2239
Hin6I	GCGC	3 cut(s)	109, 2239, 2751
HinP1I	GCGC	3 cut(s)	109, 2239, 2751
HincII	GTYRAC	1 cut(s)	235
HindII	GTYRAC	1 cut(s)	235
HindIII	AAGCTT	5 cut(s)	1406, 1806, 2057, 2292, 2609
HpaII	CCGG	1 cut(s)	2746
HphI	GGTGA	4 cut(s)	254, 797, 1337, 2813
Hpy166II	GTNNAC	4 cut(s)	71, 235, 833, 2507
Hpy8I	GTNNAC	4 cut(s)	71, 235, 833, 2507
HpyCH4III	ACNGT	6 cut(s)	242, 248, 738, 824, 1186, 2333
HpyCH4IV	ACGT	3 cut(s)	768, 1735, 2539
HpySE526I	ACGT	3 cut(s)	768, 1735, 2539
Hsp92I	GRCGYC	2 cut(s)	109, 2239
HspAI	GCGC	3 cut(s)	109, 2239, 2751
KasI	GGCGCC	2 cut(s)	108, 2238
KpnI	GGTACC	2 cut(s)	1789, 2271
Ksp22I	TGATCA	1 cut(s)	2158
LguI	GCTCTTC	1 cut(s)	2472
LmnI	GCTCC	6 cut(s)	1081, 1804, 1876, 2748, 2868, 2894
MaeI	CTAG	4 cut(s)	333, 581, 620, 2810
MaeII	ACGT	3 cut(s)	768, 1735, 2539
MfeI	CAATTG	2 cut(s)	365, 1759
MflI	RGATCY	4 cut(s)	1529, 2494, 2697, 2736
MhlI	GDGCHC	2 cut(s)	2014, 2712
MlsI	TGGCCA	1 cut(s)	2593
MluNI	TGGCCA	1 cut(s)	2593
Mly113I	GGCGCC	2 cut(s)	109, 2239
MlyI	GAGTC	4 cut(s)	838, 1037, 1054, 2032
MmeI	TCCRAC	2 cut(s)	924, 930
Mox20I	TGGCCA	1 cut(s)	2593
Mph1103I	ATGCAT	4 cut(s)	796, 967, 1962, 2629
MroXI	GAANNNNTTC	3 cut(s)	498, 1935, 2119
MscI	TGGCCA	1 cut(s)	2593
MseI	TTAA	7 cut(s)	296, 696, 816, 1356, 1412, 2394, 2489
MslI	CAYNNNNRTG	6 cut(s)	966, 1399, 1840, 1965, 2007, 2799
Msp20I	TGGCCA	1 cut(s)	2593
MspA1I	CMGCKG	5 cut(s)	32, 647, 662, 1172, 1669
MspI	CCGG	1 cut(s)	2746
MspR9I	CCNGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
MunI	CAATTG	2 cut(s)	365, 1759
Mva1269I	GAATGC	1 cut(s)	2306
MvaI	CCWGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
NarI	GGCGCC	2 cut(s)	109, 2239
NdeI	CATATG	2 cut(s)	967, 1918
NheI	GCTAGC	1 cut(s)	580
NlaIV	GGNNCC	7 cut(s)	110, 1787, 1847, 2070, 2240, 2269, 2738
NmeAIII	GCCGAG	1 cut(s)	1892
NmuCI	GTSAC	4 cut(s)	242, 785, 2437, 2801
NsiI	ATGCAT	4 cut(s)	796, 967, 1962, 2629
NspI	RCATGY	3 cut(s)	387, 1613, 1964
NspV	TTCGAA	1 cut(s)	2297
PaeI	GCATGC	2 cut(s)	1613, 1964
PagI	TCATGA	1 cut(s)	1966
PciSI	GCTCTTC	1 cut(s)	2472
PcsI	WCGNNNNNNNCGW	1 cut(s)	2829
PctI	GAATGC	1 cut(s)	2306
PdmI	GAANNNNTTC	3 cut(s)	498, 1935, 2119
PflMI	CCANNNNNTGG	2 cut(s)	1683, 1724
PfoI	TCCNGGA	1 cut(s)	2644
PleI	GAGTC	4 cut(s)	837, 1036, 1053, 2031
PluTI	GGCGCC	2 cut(s)	112, 2242
PmaCI	CACGTG	1 cut(s)	2540
PmlI	CACGTG	1 cut(s)	2540
PpsI	GAGTC	4 cut(s)	837, 1036, 1053, 2031
Ppu21I	YACGTR	1 cut(s)	2540
PshAI	GACNNNNGTC	1 cut(s)	2685
Psp124BI	GAGCTC	1 cut(s)	2712
Psp6I	CCWGG	6 cut(s)	204, 762, 1113, 1321, 1717, 2644
PspCI	CACGTG	1 cut(s)	2540
PspEI	GGTNACC	1 cut(s)	785
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	6 cut(s)	204, 762, 1113, 1321, 1717, 2644
PspN4I	GGNNCC	7 cut(s)	110, 1787, 1847, 2070, 2240, 2269, 2738
PspPI	GGNCC	8 cut(s)	760, 853, 958, 985, 1421, 2069, 2107, 2532
PstI	CTGCAG	2 cut(s)	492, 1294
PstNI	CAGNNNCTG	4 cut(s)	487, 665, 1289, 1724
PsuI	RGATCY	4 cut(s)	1529, 2494, 2697, 2736
PvuII	CAGCTG	3 cut(s)	32, 662, 1172
RsaI	GTAC	8 cut(s)	567, 715, 1188, 1465, 1474, 1787, 1924, 2269
RsaNI	GTAC	8 cut(s)	566, 714, 1187, 1464, 1473, 1786, 1923, 2268
RseI	CAYNNNNRTG	6 cut(s)	966, 1399, 1840, 1965, 2007, 2799
SacI	GAGCTC	1 cut(s)	2712
SapI	GCTCTTC	1 cut(s)	2472
SaqAI	TTAA	7 cut(s)	296, 696, 816, 1356, 1412, 2394, 2489
Sau96I	GGNCC	8 cut(s)	760, 853, 958, 985, 1421, 2069, 2107, 2532
SchI	GAGTC	4 cut(s)	838, 1037, 1054, 2032
ScrFI	CCNGG	6 cut(s)	206, 764, 1115, 1323, 1719, 2646
SduI	GDGCHC	2 cut(s)	2014, 2712
SfcI	CTRYAG	6 cut(s)	488, 657, 750, 875, 1290, 2409
SfoI	GGCGCC	2 cut(s)	110, 2240
SfuI	TTCGAA	1 cut(s)	2297
SinI	GGWCC	3 cut(s)	853, 985, 1421
SmiMI	CAYNNNNRTG	6 cut(s)	966, 1399, 1840, 1965, 2007, 2799
SmlI	CTYRAG	6 cut(s)	455, 980, 1210, 1513, 1652, 1875
SmoI	CTYRAG	6 cut(s)	455, 980, 1210, 1513, 1652, 1875
SphI	GCATGC	2 cut(s)	1613, 1964
SsiI	CCGC	7 cut(s)	103, 106, 563, 645, 1669, 1952, 2415
SspDI	GGCGCC	2 cut(s)	108, 2238
SspI	AATATT	4 cut(s)	720, 2155, 2324, 2480
SspMI	CTAG	4 cut(s)	333, 581, 620, 2810
SstI	GAGCTC	1 cut(s)	2712
StyD4I	CCNGG	6 cut(s)	204, 762, 1113, 1321, 1717, 2644
StyI	CCWWGG	2 cut(s)	1487, 1980
TaaI	ACNGT	6 cut(s)	242, 248, 738, 824, 1186, 2333
TaiI	ACGT	3 cut(s)	771, 1738, 2542
TaqI	TCGA	7 cut(s)	134, 725, 740, 2005, 2123, 2297, 2832
TaqII	GACCGA	1 cut(s)	841
TatI	WGTACW	1 cut(s)	1186
TauI	GCSGC	3 cut(s)	106, 109, 2418
Tru1I	TTAA	7 cut(s)	296, 696, 816, 1356, 1412, 2394, 2489
Tru9I	TTAA	7 cut(s)	296, 696, 816, 1356, 1412, 2394, 2489
TscAI	CASTG	5 cut(s)	223, 1233, 1787, 1845, 1949
TseFI	GTSAC	4 cut(s)	242, 785, 2437, 2801
Tsp45I	GTSAC	4 cut(s)	242, 785, 2437, 2801
TspDTI	ATGAA	7 cut(s)	282, 1089, 1103, 2358, 2459, 2793, 2831
TspGWI	ACGGA	3 cut(s)	1460, 1855, 1874
TspRI	CASTG	5 cut(s)	223, 1233, 1787, 1845, 1949
Van91I	CCANNNNNTGG	2 cut(s)	1683, 1724
VpaK11BI	GGWCC	3 cut(s)	853, 985, 1421
XapI	RAATTY	4 cut(s)	973, 1019, 2098, 2451
XceI	RCATGY	3 cut(s)	387, 1613, 1964
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2034
XmnI	GAANNNNTTC	3 cut(s)	498, 1935, 2119
XspI	CTAG	4 cut(s)	333, 581, 620, 2810
Zsp2I	ATGCAT	4 cut(s)	796, 967, 1962, 2629

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.