Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0003013

Physical Location & Seq

Genomic Coordinates Forward (+)

84083171 .. 84090687

Gene Structure 7517 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG606800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGGGTGTTATTCCATTGCCTGATATGGCAGCTGGGGTTATTCCCAACCTCGCCGTGTCATATTCAGGTGGACATTGCAAACATGATACGACTTTATTCCTGCGGCGGCGGCACCCTCTTGTTTTCAGAACAAACTCGAATAGAAAAAAGAAACTATCCCTCACAGAACATTGGCTATGTAAATCAAGAACAAGCATATTTTCAAGCCTGGATGATTCCAGTGTTACAGATGTTGTTGACAACAGTGACAGTTTTTCTTCAGGAAGAAATGAAATGTTGAGTGTAGAGGAGGATGAGTTAATGACAGCTAAAATAGCACTTTCTGAGGCTCATGCTAGACAAGAAGCCATTGAGAAAGAGAGGGATCAATTGCTTGAAGAGTTGGCATGTTTTGAGGCAAAACAACAGGAATATATAGCTACCATATTGCACGACAAGGATATGGCTGTTTCGGAACTTGAGGCTGCAAAATCTTTGTTCAATCAGAAGCTGCAGGAATCAGTTCAAGAGAAGTTCAGCTTGCAAGATAAGTTAGTCCTTGTGAAACAAGATGCTGTTCAACTTGCGGTTCAAGTAGAGAGGCTAGCAGAAATTGCTTTTCGGCAGGCAACATCTCACATACTAGAAGATGCCCAGTTGAGGGTTTCCGCTGCTGAAACTACAGCTGCTGAAGCATCTTATCAGATAGAAAAGCAAATTAAGGATATGACTGAAGGTACTATATTGTCGATTGTGGAACAGTCGAAAAATGCTATAGAGAAGGCCCTGGACGTGGCAGAAAAAGCTGGTGACCATGCAACAAAAGCTGCGTCAGCATTTAATCACAGTATGAGTCCGCTTGATGAGCTTGCTTCGGTCCAGTCAAAGAACATCATGCTACAGGGTGCTGTGAATGATTTAGAATCTCAGTTACTGCTTACAAAAAGTGACGTCGCTAAGTTGAAGTTGGAGTTGGAAAAGGCCCATGCATATGCAAATTTGCTTGAGGTCCGAGCTACTGATGCTGAGAAAGCATTGGTTGAATTTCAGGAGTCAAGCAGGAAAGAGAGTCTCCAGAGAGAGCAGGAAATCATGTCATTGATGGAGCAGATGAAGAAAGATTCATCGGAAAGAAACCAGGCTGCTTCAGGGGCTTTCAATGTTGAGTTACAAAGCATTAGGGATGCTGTTGCAGCTGTGAAAGAAACGGTACATTCCAAAGATGATGCCTACTTGAGAAGATGCGAAGCACTGCAAAGATCGTTGAAGGCATCTGAAGCTACTACAAAGATGTGGAGACAGAGAGCAGAAACTGCAGAATCCTTGTTATTGAAGGAAAGGCAGCCAGGTGATTGGGAAGAAGATTCCATTTTTGTTGTTAATGGTGGAAGGATAGACCTTTTGACCAATGATGATTCACAGAAATGGAAACTTTTAAGTGATGGTCCTCGCAGAGAGATACCTCAATGGATGGCAAGGAGAATATGTACTATCCGTACCAAGTTTCCTCCAAGGAAGATTGATGTAGCTGAAGCCTTGAGTTCAGAGTTCAGATCTTTGAACTTGCCAAAGCCTGAGGAAGTATGGTCAATAGCTCTGGAAAAGCCAAAGGACGGGGATACACTTATTGAGCATGCTTTTGAGAAAGAAACAATAGAGAAGAAAAGGAAGGCTCTTGAGCGTGTTCTTCAGCGGAAGACCACACAATGGCAGAGGACTGAGGAACAAACAAAATTAGAGCCAGGAACTGGAACAGGACGTGAAATTGTGTTTCAAGGTTTCAATTGGGAAAGCTGGAGAAAGCAGTGGTACCTTGATTTAGCTCCTAAAGCTTCTGATTTATCTAAAATTGGGGTAACATCAGTGTGGTTCCCACCACCAACGGAATCTGTTGCTCCTCAAGGTTATATGCCTTCTGACCTTTACAATTTGAACTCGGCATATGGTACTAAGGAAGAACTTCAACACTGCATTGCAGAGATGCATGCTCATGATCTTCTGGCCTTGGGAGATGTTGTCCTGAATCATCGATGTGCTCATAAACAGAGTCCAAATGGTGTTTGGAACATCTTTGGTGGGAAGCTTGCATGGGGGCCTGAAGCAATCGTTTGTGATGATCCAAATTTTGAGGGCCGTGGAAACCCTTCGAGTGGGGATATATTCCATGCAGCACCCAATATTGATCATTCAAAGGACTTTGTAAGAAAAGATATAAAGGAGTGGCTAAACTGGCTTAGAAGTGATATTGGTTTTGATGGATGGCGCCTTGATTTTGTAAGAGGCTTCTCTGGTACCCATGTAAAGGAATATATTGAAGCTTCAAATCCAGCATTTGCTATTGGAGAATATTGGGACAGTTTAGCTTATGAAAATGGAAGTTTGTGTTACAATCAAGATGCTCATCGCCAGAGGATAGTTAATTGGATCAATGCTACAGGCGGCAGTTCCTCAGCATTTGATGTCACAACTAAGGGAATTCTCCATTCTGCTCTTCATAACCAATATTGGAGGTTAATAGATCCTCAGGGTAAACCAACTGGAGTTATGGGATGGTGGCCCTCACGTGCTGTTACATTTTTGGAGAACCATGACACAGGATCAACACAGGGTCATTGGCCATTTCCACGAGAAAAGCTTACACAGGGATATGCATACATTTTGACCCATCCTGGAACACCTACCATCTTTTACGACCATTTATACGACTTTGGTCTTCATGAGATCCTAAGTGAGCTCATTGAGGCTCGGAGAAGGGCAGGGATCCATTGCCGGAGCGCCGTGAAGATATACCATGCAAACAATGAAGGGTATGTTGCACATATAGGTGACACTCTAGTAATGAAGCTCGGACATTTCGACTGGAATCCCTCAAAGGAAAACCATTTGGAAGGGAGCTGGCAGAAGTTTGTTGATAAAGGAGCAGATTACATAGTGTGGTTACGACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

975

Amino Acids

110.32

Weight (kDa)

5.78

Isoelectric Point (pI)

48.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	607 - 671	9.5e-09	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	911 - 972	6.2e-16	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15550 AT5G15550 AT5G15550
fragaria_vesca	FvH4_6g46921 FvH4_6g46921 FvH4_6g46921 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930
malus_domestica	MD09G1066000.v1.1 MD09G1066100.v1.1 MD17G1058700.v1.1 MD17G1058800.v1.1
prunus_persica	Prupe.3G255400_v2.0.a1 Prupe.3G255500_v2.0.a1
pyrus_communis	pycom111g05550 pycom17g05870
rosa_chinensis	RchiOBHm_Chr2g0165901 RchiOBHm_Chr2g0165911
rosa_laevigata	RLG00000021569
rosa_multiflora	Rmu_co8422767.1_g000001 Rmu_sc0006218.1_g000010
rosa_roxburghii	Rroxscaffold_2G00085310 Rroxscaffold_2G00085320
rosa_rugosa	Rorug02G0519200 Rorug02G0519300
rosa_samantha	Rh2AG584700 Rh2AG584800 Rh2BG596600 Rh2CG567400 Rh2DG606700 Rh2DG606800
rosa_wichuraiana	Rw2G048750 Rw2G048760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	933
Acc65I	GGTACC	2 cut(s)	1788, 2270
AccB1I	GGYRCC	4 cut(s)	111, 1788, 2241, 2270
AccB7I	CCANNNNNTGG	2 cut(s)	1686, 1727
AciI	CCGC	8 cut(s)	103, 106, 109, 566, 648, 836, 1672, 2418
AclWI	GGATC	8 cut(s)	372, 2090, 2411, 2492, 2584, 2695, 2734, 2747
AcoI	YGGCCR	1 cut(s)	2594
AcsI	RAATTY	4 cut(s)	976, 1022, 2101, 2454
AcuI	CTGAAG	8 cut(s)	243, 690, 732, 1110, 1275, 1530, 1652, 2097
AcvI	CACGTG	1 cut(s)	2543
AcyI	GRCGYC	2 cut(s)	930, 2242
AfaI	GTAC	7 cut(s)	718, 1191, 1468, 1477, 1790, 1927, 2272
AfiI	CCNNNNNNNGG	8 cut(s)	639, 640, 772, 1592, 1686, 1727, 2129, 2281
AjiI	CACGTC	2 cut(s)	772, 1739
AjnI	CCWGG	6 cut(s)	207, 765, 1116, 1324, 1720, 2647
AjuI	GAANNNNNNNTTGG	2 cut(s)	935, 967
Alw21I	GWGCWC	2 cut(s)	2017, 2715
Alw26I	GTCTC	2 cut(s)	1055, 1270
AlwI	GGATC	8 cut(s)	372, 2090, 2411, 2492, 2584, 2695, 2734, 2747
AlwNI	CAGNNNCTG	5 cut(s)	490, 668, 913, 1292, 1727
AoxI	GGCC	7 cut(s)	762, 960, 1980, 2072, 2110, 2534, 2594
ApoI	RAATTY	4 cut(s)	976, 1022, 2101, 2454
ArsI	GACNNNNNNTTYG	2 cut(s)	794, 826
Asp700I	GAANNNNTTC	3 cut(s)	501, 1938, 2122
Asp718I	GGTACC	2 cut(s)	1788, 2270
AspLEI	GCGC	2 cut(s)	2244, 2756
AspS9I	GGNCC	8 cut(s)	763, 856, 961, 988, 1424, 2072, 2110, 2535
AsuHPI	GGTGA	3 cut(s)	800, 1340, 2816
AsuNHI	GCTAGC	1 cut(s)	583
AvaII	GGWCC	3 cut(s)	856, 988, 1424
AxyI	CCTNAGG	2 cut(s)	1554, 2502
BaeI	ACNNNNGTAYC	2 cut(s)	2262, 2295
BalI	TGGCCA	1 cut(s)	2596
BamHI	GGATCC	1 cut(s)	2739
BanI	GGYRCC	4 cut(s)	111, 1788, 2241, 2270
BanII	GRGCYC	1 cut(s)	2715
BauI	CACGAG	1 cut(s)	2604
BbrPI	CACGTG	1 cut(s)	2543
BbsI	GAAGAC	2 cut(s)	1682, 2684
Bbv12I	GWGCWC	2 cut(s)	2017, 2715
BbvCI	CCTCAGC	1 cut(s)	2428
BccI	CCATC	8 cut(s)	1075, 1415, 1444, 2228, 2232, 2523, 2652, 2669
BceAI	ACGGC	3 cut(s)	38, 2097, 2741
BciT130I	CCWGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
BciVI	GTATCC	1 cut(s)	1591
BclI	TGATCA	1 cut(s)	2161
BcoDI	GTCTC	2 cut(s)	1055, 1270
BfaI	CTAG	4 cut(s)	336, 584, 623, 2813
BfmI	CTRYAG	6 cut(s)	491, 660, 753, 878, 1293, 2412
BfoI	RGCGCY	2 cut(s)	2245, 2757
BfuI	GTATCC	1 cut(s)	1591
BglII	AGATCT	1 cut(s)	1532
Bme1390I	CCNGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
Bme18I	GGWCC	3 cut(s)	856, 988, 1424
BmgBI	CACGTC	2 cut(s)	772, 1739
BmgT120I	GGNCC	8 cut(s)	763, 856, 961, 988, 1424, 2072, 2110, 2535
BmiI	GGNNCC	7 cut(s)	113, 1790, 1850, 2073, 2243, 2272, 2741
BmrFI	CCNGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
BmrI	ACTGGG	1 cut(s)	628
BmtI	GCTAGC	1 cut(s)	587
BmuI	ACTGGG	1 cut(s)	628
BoxI	GACNNNNGTC	1 cut(s)	2688
BpiI	GAAGAC	2 cut(s)	1682, 2684
BpmI	CTGGAG	3 cut(s)	1037, 1795, 2538
Bpu10I	CCTNAGC	1 cut(s)	2428
BpuEI	CTTGAG	6 cut(s)	479, 1004, 1234, 1537, 1676, 1863
Bsa29I	ATCGAT	1 cut(s)	2008
BsaAI	YACGTR	1 cut(s)	2543
BsaBI	GATNNNNATC	1 cut(s)	2379
BsaHI	GRCGYC	2 cut(s)	930, 2242
BsaJI	CCNNGG	4 cut(s)	765, 1490, 1983, 2113
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 311, 1450, 1480, 1980, 2010
Bsc4I	CCNNNNNNNGG	8 cut(s)	639, 640, 772, 1592, 1686, 1727, 2129, 2281
Bse1I	ACTGG	7 cut(s)	219, 634, 859, 1732, 2213, 2521, 2843
Bse21I	CCTNAGG	2 cut(s)	1554, 2502
Bse3DI	GCAATG	4 cut(s)	14, 73, 1950, 2743
Bse8I	GATNNNNATC	1 cut(s)	2379
BseBI	CCWGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
BseCI	ATCGAT	1 cut(s)	2008
BseDI	CCNNGG	4 cut(s)	765, 1490, 1983, 2113
BseGI	GGATG	7 cut(s)	217, 298, 1168, 1455, 2243, 2534, 2644
BseJI	GATNNNNATC	1 cut(s)	2379
BseLI	CCNNNNNNNGG	8 cut(s)	639, 640, 772, 1592, 1686, 1727, 2129, 2281
BseMI	GCAATG	4 cut(s)	14, 73, 1950, 2743
BseMII	CTCAG	7 cut(s)	315, 920, 996, 1545, 1689, 2442, 2516
BseNI	ACTGG	7 cut(s)	219, 634, 859, 1732, 2213, 2521, 2843
BseRI	GAGGAG	2 cut(s)	302, 1866
BseYI	CCCAGC	1 cut(s)	32
BshFI	GGCC	7 cut(s)	764, 962, 1982, 2074, 2112, 2536, 2596
BshNI	GGYRCC	4 cut(s)	111, 1788, 2241, 2270
BshVI	ATCGAT	1 cut(s)	2008
BsiHKAI	GWGCWC	2 cut(s)	2017, 2715
BsiSI	CCGG	1 cut(s)	2749
BslFI	GGGAC	1 cut(s)	2345
BslI	CCNNNNNNNGG	8 cut(s)	639, 640, 772, 1592, 1686, 1727, 2129, 2281
BsmAI	GTCTC	2 cut(s)	1055, 1270
BsmFI	GGGAC	1 cut(s)	2345
BsnI	GGCC	7 cut(s)	764, 962, 1982, 2074, 2112, 2536, 2596
Bsp1286I	GDGCHC	2 cut(s)	2017, 2715
BspACI	CCGC	8 cut(s)	103, 106, 109, 566, 648, 836, 1672, 2418
BspANI	GGCC	7 cut(s)	764, 962, 1982, 2074, 2112, 2536, 2596
BspCNI	CTCAG	7 cut(s)	316, 919, 997, 1546, 1690, 2441, 2515
BspDI	ATCGAT	1 cut(s)	2008
BspHI	TCATGA	2 cut(s)	1969, 2695
BspLI	GGNNCC	7 cut(s)	113, 1790, 1850, 2073, 2243, 2272, 2741
BspMAI	CTGCAG	2 cut(s)	495, 1297
BspOI	GCTAGC	1 cut(s)	587
BspPI	GGATC	8 cut(s)	372, 2090, 2411, 2492, 2584, 2695, 2734, 2747
BspQI	GCTCTTC	1 cut(s)	2475
BspT107I	GGYRCC	4 cut(s)	111, 1788, 2241, 2270
BsrDI	GCAATG	4 cut(s)	14, 73, 1950, 2743
BsrI	ACTGG	7 cut(s)	219, 634, 859, 1732, 2213, 2521, 2843
BssECI	CCNNGG	4 cut(s)	765, 1490, 1983, 2113
BssNI	GRCGYC	2 cut(s)	930, 2242
BssSI	CACGAG	1 cut(s)	2604
BssT1I	CCWWGG	2 cut(s)	1490, 1983
Bst2BI	CACGAG	1 cut(s)	2604
Bst2UI	CCWGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
Bst4CI	ACNGT	6 cut(s)	245, 251, 741, 827, 1189, 2336
Bst6I	CTCTTC	2 cut(s)	372, 2475
BstACI	GRCGYC	2 cut(s)	930, 2242
BstAPI	GCANNNNNTGC	2 cut(s)	593, 1292
BstBAI	YACGTR	1 cut(s)	2543
BstC8I	GCNNGC	8 cut(s)	521, 585, 606, 849, 1614, 1965, 2064, 2876
BstDSI	CCRYGG	1 cut(s)	2113
BstEII	GGTNACC	1 cut(s)	788
BstF5I	GGATG	7 cut(s)	217, 298, 1168, 1455, 2243, 2534, 2644
BstH2I	RGCGCY	2 cut(s)	2245, 2757
BstHHI	GCGC	2 cut(s)	2244, 2756
BstMAI	GTCTC	2 cut(s)	1055, 1270
BstNI	CCWGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
BstNSI	RCATGY	3 cut(s)	390, 1616, 1967
BstPAI	GACNNNNGTC	1 cut(s)	2688
BstPI	GGTNACC	1 cut(s)	788
BstSCI	CCNGG	6 cut(s)	207, 765, 1116, 1324, 1720, 2647
BstSFI	CTRYAG	6 cut(s)	491, 660, 753, 878, 1293, 2412
BstV2I	GAAGAC	2 cut(s)	1682, 2684
BstX2I	RGATCY	4 cut(s)	1532, 2497, 2700, 2739
BstXI	CCANNNNNNTGG	1 cut(s)	1332
BstYI	RGATCY	4 cut(s)	1532, 2497, 2700, 2739
Bsu15I	ATCGAT	1 cut(s)	2008
Bsu36I	CCTNAGG	2 cut(s)	1554, 2502
BsuI	GTATCC	1 cut(s)	1591
BsuRI	GGCC	7 cut(s)	764, 962, 1982, 2074, 2112, 2536, 2596
BsuTUI	ATCGAT	1 cut(s)	2008
BtgI	CCRYGG	1 cut(s)	2113
BtgZI	GCGATG	1 cut(s)	2366
BtrI	CACGTC	2 cut(s)	772, 1739
BtsCI	GGATG	7 cut(s)	217, 298, 1168, 1455, 2243, 2534, 2644
BtsI	GCAGTG	3 cut(s)	1229, 1790, 1945
BtsIMutI	CAGTG	6 cut(s)	226, 250, 1229, 1790, 1848, 1945
Cac8I	GCNNGC	8 cut(s)	521, 585, 606, 849, 1614, 1965, 2064, 2876
CaiI	CAGNNNCTG	5 cut(s)	490, 668, 913, 1292, 1727
CciI	TCATGA	2 cut(s)	1969, 2695
CfoI	GCGC	2 cut(s)	2244, 2756
Cfr13I	GGNCC	8 cut(s)	763, 856, 961, 988, 1424, 2072, 2110, 2535
ClaI	ATCGAT	1 cut(s)	2008
CseI	GACGC	1 cut(s)	798
Csp6I	GTAC	7 cut(s)	717, 1190, 1467, 1476, 1789, 1926, 2271
CviQI	GTAC	7 cut(s)	717, 1190, 1467, 1476, 1789, 1926, 2271
DinI	GGCGCC	1 cut(s)	2243
EaeI	YGGCCR	1 cut(s)	2594
Eam1104I	CTCTTC	2 cut(s)	372, 2475
EarI	CTCTTC	2 cut(s)	372, 2475
Ecl136II	GAGCTC	1 cut(s)	2713
Eco130I	CCWWGG	2 cut(s)	1490, 1983
Eco24I	GRGCYC	1 cut(s)	2715
Eco47I	GGWCC	3 cut(s)	856, 988, 1424
Eco53kI	GAGCTC	1 cut(s)	2713
Eco57I	CTGAAG	8 cut(s)	243, 690, 732, 1110, 1275, 1530, 1652, 2097
Eco72I	CACGTG	1 cut(s)	2543
Eco81I	CCTNAGG	2 cut(s)	1554, 2502
Eco91I	GGTNACC	1 cut(s)	788
EcoICRI	GAGCTC	1 cut(s)	2713
EcoO109I	RGGNCCY	2 cut(s)	763, 2072
EcoO65I	GGTNACC	1 cut(s)	788
EcoRI	GAATTC	1 cut(s)	2454
EcoRII	CCWGG	6 cut(s)	207, 765, 1116, 1324, 1720, 2647
EcoT14I	CCWWGG	2 cut(s)	1490, 1983
EcoT22I	ATGCAT	3 cut(s)	970, 1965, 2632
EcoT38I	GRGCYC	1 cut(s)	2715
EgeI	GGCGCC	1 cut(s)	2243
EheI	GGCGCC	1 cut(s)	2243
ErhI	CCWWGG	2 cut(s)	1490, 1983
FalI	AAGNNNNNCTT	2 cut(s)	503, 535
FaqI	GGGAC	1 cut(s)	2345
FauNDI	CATATG	2 cut(s)	970, 1921
FbaI	TGATCA	1 cut(s)	2161
FokI	GGATG	7 cut(s)	224, 305, 1175, 1462, 2250, 2541, 2631
FriOI	GRGCYC	1 cut(s)	2715
FspBI	CTAG	4 cut(s)	336, 584, 623, 2813
GlaI	GCGC	2 cut(s)	2243, 2755
GsaI	CCCAGC	1 cut(s)	36
GsuI	CTGGAG	3 cut(s)	1037, 1795, 2538
HaeII	RGCGCY	2 cut(s)	2245, 2757
HaeIII	GGCC	7 cut(s)	764, 962, 1982, 2074, 2112, 2536, 2596
HapII	CCGG	1 cut(s)	2749
HgaI	GACGC	1 cut(s)	798
HhaI	GCGC	2 cut(s)	2244, 2756
Hin1I	GRCGYC	2 cut(s)	930, 2242
Hin6I	GCGC	2 cut(s)	2242, 2754
HinP1I	GCGC	2 cut(s)	2242, 2754
HincII	GTYRAC	1 cut(s)	238
HindII	GTYRAC	1 cut(s)	238
HindIII	AAGCTT	4 cut(s)	1809, 2060, 2295, 2612
HpaII	CCGG	1 cut(s)	2749
HphI	GGTGA	3 cut(s)	800, 1340, 2816
Hpy166II	GTNNAC	3 cut(s)	71, 238, 2510
Hpy8I	GTNNAC	3 cut(s)	71, 238, 2510
Hpy99I	CGWCG	1 cut(s)	935
HpyCH4III	ACNGT	6 cut(s)	245, 251, 741, 827, 1189, 2336
HpyCH4IV	ACGT	4 cut(s)	771, 930, 1738, 2542
HpySE526I	ACGT	4 cut(s)	771, 930, 1738, 2542
Hsp92I	GRCGYC	2 cut(s)	930, 2242
HspAI	GCGC	2 cut(s)	2242, 2754
KasI	GGCGCC	1 cut(s)	2241
KpnI	GGTACC	2 cut(s)	1792, 2274
Ksp22I	TGATCA	1 cut(s)	2161
LguI	GCTCTTC	1 cut(s)	2475
LmnI	GCTCC	6 cut(s)	1084, 1807, 1879, 2751, 2871, 2897
MaeI	CTAG	4 cut(s)	336, 584, 623, 2813
MaeII	ACGT	4 cut(s)	771, 930, 1738, 2542
MfeI	CAATTG	2 cut(s)	368, 1762
MflI	RGATCY	4 cut(s)	1532, 2497, 2700, 2739
MhlI	GDGCHC	2 cut(s)	2017, 2715
MlsI	TGGCCA	1 cut(s)	2596
MluNI	TGGCCA	1 cut(s)	2596
Mly113I	GGCGCC	1 cut(s)	2242
MlyI	GAGTC	4 cut(s)	841, 1040, 1057, 2035
MmeI	TCCRAC	2 cut(s)	927, 933
Mox20I	TGGCCA	1 cut(s)	2596
Mph1103I	ATGCAT	3 cut(s)	970, 1965, 2632
MroXI	GAANNNNTTC	3 cut(s)	501, 1938, 2122
MscI	TGGCCA	1 cut(s)	2596
MseI	TTAA	7 cut(s)	299, 699, 819, 1359, 1415, 2397, 2492
MslI	CAYNNNNRTG	6 cut(s)	969, 1402, 1843, 1968, 2010, 2802
Msp20I	TGGCCA	1 cut(s)	2596
MspA1I	CMGCKG	5 cut(s)	32, 650, 665, 1175, 1672
MspI	CCGG	1 cut(s)	2749
MspR9I	CCNGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
MunI	CAATTG	2 cut(s)	368, 1762
MvaI	CCWGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
NarI	GGCGCC	1 cut(s)	2242
NdeI	CATATG	2 cut(s)	970, 1921
NheI	GCTAGC	1 cut(s)	583
NlaIV	GGNNCC	7 cut(s)	113, 1790, 1850, 2073, 2243, 2272, 2741
NmeAIII	GCCGAG	1 cut(s)	1895
NmuCI	GTSAC	5 cut(s)	245, 788, 926, 2440, 2804
NsiI	ATGCAT	3 cut(s)	970, 1965, 2632
NspI	RCATGY	3 cut(s)	390, 1616, 1967
PaeI	GCATGC	2 cut(s)	1616, 1967
PagI	TCATGA	2 cut(s)	1969, 2695
PciSI	GCTCTTC	1 cut(s)	2475
PcsI	WCGNNNNNNNCGW	1 cut(s)	2832
PdmI	GAANNNNTTC	3 cut(s)	501, 1938, 2122
PflMI	CCANNNNNTGG	2 cut(s)	1686, 1727
PfoI	TCCNGGA	1 cut(s)	2647
PleI	GAGTC	4 cut(s)	840, 1039, 1056, 2034
PluTI	GGCGCC	1 cut(s)	2245
PmaCI	CACGTG	1 cut(s)	2543
PmlI	CACGTG	1 cut(s)	2543
PpsI	GAGTC	4 cut(s)	840, 1039, 1056, 2034
Ppu21I	YACGTR	1 cut(s)	2543
PshAI	GACNNNNGTC	1 cut(s)	2688
Psp124BI	GAGCTC	1 cut(s)	2715
Psp6I	CCWGG	6 cut(s)	207, 765, 1116, 1324, 1720, 2647
PspCI	CACGTG	1 cut(s)	2543
PspEI	GGTNACC	1 cut(s)	788
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	6 cut(s)	207, 765, 1116, 1324, 1720, 2647
PspN4I	GGNNCC	7 cut(s)	113, 1790, 1850, 2073, 2243, 2272, 2741
PspPI	GGNCC	8 cut(s)	763, 856, 961, 988, 1424, 2072, 2110, 2535
PstI	CTGCAG	2 cut(s)	495, 1297
PstNI	CAGNNNCTG	5 cut(s)	490, 668, 913, 1292, 1727
PsuI	RGATCY	4 cut(s)	1532, 2497, 2700, 2739
PvuII	CAGCTG	3 cut(s)	32, 665, 1175
RsaI	GTAC	7 cut(s)	718, 1191, 1468, 1477, 1790, 1927, 2272
RsaNI	GTAC	7 cut(s)	717, 1190, 1467, 1476, 1789, 1926, 2271
RseI	CAYNNNNRTG	6 cut(s)	969, 1402, 1843, 1968, 2010, 2802
SacI	GAGCTC	1 cut(s)	2715
SapI	GCTCTTC	1 cut(s)	2475
SaqAI	TTAA	7 cut(s)	299, 699, 819, 1359, 1415, 2397, 2492
Sau96I	GGNCC	8 cut(s)	763, 856, 961, 988, 1424, 2072, 2110, 2535
SchI	GAGTC	4 cut(s)	841, 1040, 1057, 2035
ScrFI	CCNGG	6 cut(s)	209, 767, 1118, 1326, 1722, 2649
SduI	GDGCHC	2 cut(s)	2017, 2715
SfcI	CTRYAG	6 cut(s)	491, 660, 753, 878, 1293, 2412
SfoI	GGCGCC	1 cut(s)	2243
SinI	GGWCC	3 cut(s)	856, 988, 1424
SmiMI	CAYNNNNRTG	6 cut(s)	969, 1402, 1843, 1968, 2010, 2802
SmlI	CTYRAG	6 cut(s)	458, 983, 1213, 1516, 1655, 1878
SmoI	CTYRAG	6 cut(s)	458, 983, 1213, 1516, 1655, 1878
SphI	GCATGC	2 cut(s)	1616, 1967
SsiI	CCGC	8 cut(s)	103, 106, 109, 566, 648, 836, 1672, 2418
SspDI	GGCGCC	1 cut(s)	2241
SspI	AATATT	3 cut(s)	2158, 2327, 2483
SspMI	CTAG	4 cut(s)	336, 584, 623, 2813
SstI	GAGCTC	1 cut(s)	2715
StyD4I	CCNGG	6 cut(s)	207, 765, 1116, 1324, 1720, 2647
StyI	CCWWGG	2 cut(s)	1490, 1983
TaaI	ACNGT	6 cut(s)	245, 251, 741, 827, 1189, 2336
TaiI	ACGT	4 cut(s)	774, 933, 1741, 2545
TaqI	TCGA	6 cut(s)	137, 728, 743, 2008, 2126, 2835
TaqII	GACCGA	1 cut(s)	844
TatI	WGTACW	1 cut(s)	1466
TauI	GCSGC	4 cut(s)	106, 109, 112, 2421
Tru1I	TTAA	7 cut(s)	299, 699, 819, 1359, 1415, 2397, 2492
Tru9I	TTAA	7 cut(s)	299, 699, 819, 1359, 1415, 2397, 2492
TscAI	CASTG	6 cut(s)	226, 250, 1236, 1790, 1848, 1952
TseFI	GTSAC	5 cut(s)	245, 788, 926, 2440, 2804
Tsp45I	GTSAC	5 cut(s)	245, 788, 926, 2440, 2804
TspDTI	ATGAA	8 cut(s)	285, 1092, 1106, 2361, 2462, 2684, 2796, 2834
TspGWI	ACGGA	2 cut(s)	1463, 1877
TspRI	CASTG	6 cut(s)	226, 250, 1236, 1790, 1848, 1952
Van91I	CCANNNNNTGG	2 cut(s)	1686, 1727
VpaK11BI	GGWCC	3 cut(s)	856, 988, 1424
XapI	RAATTY	4 cut(s)	976, 1022, 2101, 2454
XceI	RCATGY	3 cut(s)	390, 1616, 1967
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2037
XmnI	GAANNNNTTC	3 cut(s)	501, 1938, 2122
XspI	CTAG	4 cut(s)	336, 584, 623, 2813
ZraI	GACGTC	1 cut(s)	931
Zsp2I	ATGCAT	3 cut(s)	970, 1965, 2632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.