RLG00000021569

Alpha-amylase C-terminal beta-sheet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003013
Physical Location & Seq
Forward (+)
76452146 .. 76464646
12501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021569

Sequence Viewer

Length: 4326 bp
ATGGAGATAGAGGAAGGCACAGATGACAGGCAGGTCCAGGTTCGCTTCGTCACGAAGCTTCAGGACATCCCCTTCAAAGTTCCCAACACCACCATTCCCGCCTCCTATTCCCGTTTGGACCTCTCCAAAGTCGTCAACGCGCTCATCCAAACCGCAAATCCTGACTGGGAATCGGAGCCCTTTGATTTTCTCATCAATGGCGTATTGGTTCGGATGTCGCTTGGACAGTTCCTTCTTGCTAAGGGCATTTCCGCGGAGAAGACATTGGAAATTGAATATATACGGGCTGTTGTCCCACGCAGAGAAGAAGAGCCTTGTTTACATGATGACTGGGTCGGTGCAGTTGATGGTTCCAGTCCCGGGTTCATTTTGACAGGGTGCTATGATGGTTTAGGAAGGGTATGGAAAGCTTCTGGAACATGTACACATATACTGCAAGGACACAGTGAACCAGTTACATCTGTTAGTGTTATCAGTGCAGAAGGGGGAGAAAGTTGTAGCGTAGCTACTGCTTCAAAAGATCGCACACTGAGGCTGTGGAAGTTCAATACAGAAGAGCCAACAAACAATCCTTTGAGGACAACAGCATACAAAATTTTGCGTGGGCACGGAGCAGCTGTTCAAAGTGTCGCTGCTCATACCTCTGGAGACATGGTCTGTTCAGGCTCCTGGGATTCCACAATCAAATTATGGCAGACAGATGAGCCTGATATAGAAGTTGATGTGTCAATTAAGAAGAGAAAAAAAATTGATGAAGCTAAGGAATCTCAAAAGGAGGGGGAGGCTGTTTCTTCTCTAGTGGGCCATACACAATGTGTATCTTCTGTCAAGTGGCCTCAGCGTGACATAATCTATTCGGCATCTTGGGACCATTCTATTAGAAGGTGGGATGTTGGGACAGGCAAAGATGTACTGAACATACCCGCCAGCAAAGCCCTCAACTGTCTTGATATTGGTGGTGAAGGTTCTGCACTTGTTGCTGCTGGTGGTTCTGACCCCATTCTTAGGATATGGGATCCTCGTAGACCAGGAACTTCTGCTCCTGTTGGTCAATTCTCATCTCACACTTCTTGGATTACGGGATGCAAGTGGCATGAGAGCTCTTGGTTTCATATACTCTCTTCATCTTATGATGGCAAAGTCATGCTCTGGGATATGAGAACTACTTGGCCTTTATTTGTCATTGATTCACATAAAGACAGCAAGGTATTATTTCCGGCGTTTCTGTGCAGTGAGTTAAATTTTGAAGATAGAAGTACTCACACATGGAGTGGCACATCTTCAGCCCTCCCATTTTCTGAAAATGGTGCACCTAAATCATGGGGCCTCATCACCTTCCTGTTTCGGATAATTAATCAAACCTCATTGTTTTTTTTTTTTTTTTTTTTGCATCAGGTAGAAATGGGTGTTATTTCATTGCCTGATATGGCAGCTGGGGTCATTCCCAACCTCGCCGTGTCACATTCAGGTGGACATTGGAAACATGATAGGACTTTATTCCTGCGGCGGCGGCCTCTTGTTTTCAGAACAAACTTGAATAGAAAAAAGAAACTATCCCTCACAGAACATTGGCTATGTAAATCAAGAACAAGCATATTTTCAAGCCTGGATGATTCCAGTGTTACGGATGTTGTTGACAACAGTGACAGTTTTTCTTCAGGAAGAAATGAAATGTTGAGTGTAGAGGAGGATGAGTTAATGACAGCTAAAATAGCACTTTCTGAGGCTCATGCTAGACAAGAAGCCATTGAGAAAGAGAGGGATCAATTGCTTGAAGAGTTGGCATGTTTTGAGGCAAAACAACAGGAATATGTAGCTACCATATTGCACGACAAGGATATGGCTGTTTCAGAACTTGAGGCTGCCAAATCTTTGTTCAATCAGAAGCTGCAGGAATCCGTTCAAGAGAAGTTCAGCTTGCAAGATAAGTTAGTCCTTGTGAAACAAGATGCTGTTCAACTTGCGGTACAAGTAGAGAGGTTAGCAGAAATTGCTTTTCAGCAGGCAACATCTCACATACTAGAAGATGCCCAGTTGAGGGTTTCCGCTGCTGAAACTACAGCTGCTGAAGCATCTTATCAGATAGAAAAGCAAATTAAGGATTTGACTGAAGGTACTATATTGTCGATTGTGGAACAGTCGAAAAATGCTATAGAGAAGGCCCTGGACGTGGCAGAAAAAGCTGGTGACCATGCAACAAAAGCTGCATCAGCATTTAATGACAGTATGAGTCCACTTGATGAGCTTGCTTCGGTCCAGTCAAAGAACATCATGCTACAGGATGCTGTGAATGATTTAGAATCTCAGTTATTGCTTACAAAAAGTGACGTCACTAAGTTGAAGTTGGAGTTGGAAAAGGCCCGTGCATATGCAAATTTGCTTGAGGTCCGAGCTACTGATGCTGAGAAAGCATTGGTTGAATTTCAGGAGTCAAGCAGGAAAGAGAGTCTCCAGAGAGAGCAGGAAATCATGTCATTGGTGGAGCAGATGAAGAAAGATTCATCGGAAAGAAACCAGGCTGCTTCAGGGGCTTTCAATGTTGAGTTACAAAGCATTAGGGATGCTGTTGCAGCTGTGAAAGAAACAGTACATTCCAAAGATGATGCCTACTTGAGAAGATGCGAAGCACTGCAAAGATCGTTGAAGGCATCTGAAGCTACTACAAAGATGTGGAGACAGAGAGCAGAAACTGCAGAATCCTTGTTATTGAAGGAAAGGCAGCCAGGTGATCGGGAAGAAGATTCCATTTTTGTTGTTAATGGTGGAAGGATAGACCTTTTGACCAATGATGATTCACAGAAATGGAAACTTTTAAGTGATGGTCCTCGCAGAGAGATACCTCAATGGATGGCAAGGAGAATATGTACTATCCGTACCAAGTTTCCTCCAAGGAAGATTGATGTAGCTGAAGCCTTGAGTTCAGAGTTCAGATCTTTGAACTTGCCAAAGCCTGAGGAAGTATGGTCAATAGCCCTGGAAAAGCCAAAGGAGGGGGATACACTTATTGAGCATGCTTTTGAGAAAGAAACAATAGAGAAGAAAAGGAAGGCTCTTGAGCGTGTTCTTCAGCGGAAGACCACACAATGGCAGAGGACTGAGGAACAAACAAAATTAGAGCCAGGAACTGGAACAGGACGTGAGATTGTGTTTCAAGGTTTCAATTGGGAAAGCTGGAGAAAGCAGTGGTACCTTGATTTAGCTCCTAAAGCTTCTGATTTATCTAAAATTGGGGTAACATCAGTGTGGTTCCCACCACCAACGGAATCCGTTGCTCCTCAAGGTTATATGCCTTCTGACCTTTACAATTTGAACTCAGCATATGGTACTAAGGAAGAACTTCAACACTGCATTGCGGAGATGCATGCTCATGATCTTCTGGCCTTGGGAGATGTTGTCCTGAATCATCGATGTGCTCATAAACAGAGTCCAAATGGTGTTTGGAACATCTTTGGTGGGAAGCTTGCATGGGGGCCTGAAGCAATCGTTTGTGATGATCCAAATTTTGAGGGCCGTGGCAACCCTTCAAGTGGGGATATATTCCATGCAGCACCCAATATTGATCATTCAAAGGACTTTGTAAGAAAAGATATAAAGGAGTGGCTAAATTGGCTTAGGAGTGATATTGGTTTTGATGGATGGCGCCTTGATTTTGTAAGAGGCTTCTCTGGTACCCATGTAAAGGAATATATTGAAGCTTCGAATCCAGCATTTGCTATTGGAGAATATTGGGACAGTTTAGCTTATGAAAATGGAAGTTTGTGTTACAATCAAGATGCTCATCGCCAAAGGATAGTTAATTGGATCAATGCTACAGGCGGCAGTTCCTCAGCATTTGATGTCACAACTAAGGGAATTCTCCATTCTGCTCTTCATAACCAATATTGGAGGTTAATAGATCCTCAGGGTAAACCAACTGGAGTTATGGGATGGTGGCCCTCACGTGCTGTTACATTTTTGGAGAACCATGACACAGGATCAACACAGGGTCATTGGCCATTTCCACGAGAAAAGCTTACACAGGGATATGCATACATTTTGACCCATCCTGGAACACCTACCATCTTTTACGACCATTTATACGACTTTGGTCTTCATGAGATCCTAACTGAGCTCATTGAGGCTCGGAGAAGGGCAGGGATCCATTGCCGGAGCGCTGTGAAGATATACCATGCAAACAATGAAGGGTATGTTGCACATATAGGTGACAGTCTAGTAATGAAGCTCGGACATTTCGACTGGAATCCCTCAAAGGAAAACCGTTTGGAAGGGAGCTGGCAGAAGTTTGTTGATAAAGGAGCAGATTACATAGTGTGGTTACGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

1442

Amino Acids

162.14

Weight (kDa)

5.73

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NLE PF08154 12 - 78 3.7e-14 NLE (NUC135) domain
Beta-prop_THOC3 PF25174 108 - 190 7.6e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 116 - 238 1.4e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 119 - 234 1.3e-15 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 119 - 237 7.7e-08 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 149 - 311 6.5e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 169 - 238 1.5e-09 THOC3 beta-propeller domain
WD40 PF00400 198 - 231 3.1e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 203 - 312 3.5e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 258 - 340 1.3e-09 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 260 - 400 3.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 312 - 399 2.7e-08 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 312 - 398 1.7e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 313 - 401 4.9e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 323 - 401 7.2e-08 WDR5 beta-propeller domain
Alpha-amylase PF00128 1073 - 1137 1.5e-08 Alpha amylase, catalytic domain
Alpha-amyl_C2 PF07821 1377 - 1438 7.6e-16 Alpha-amylase C-terminal beta-sheet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AatII GACGTC 1 cut(s) 2331
Acc36I ACCTGC 1 cut(s) 22
Acc65I GGTACC 2 cut(s) 3186, 3668
AccB1I GGYRCC 3 cut(s) 3186, 3639, 3668
AccB7I CCANNNNNTGG 2 cut(s) 3084, 3125
AccI GTMKAC 1 cut(s) 1024
AccII CGCG 2 cut(s) 140, 254
AcoI YGGCCR 1 cut(s) 3992
AcsI RAATTY 6 cut(s) 594, 1240, 2374, 2420, 3499, 3852
AcvI CACGTG 1 cut(s) 3941
AcyI GRCGYC 2 cut(s) 2328, 3640
AdeI CACNNNGTG 1 cut(s) 815
AfeI AGCGCT 1 cut(s) 4153
AflIII ACRYGT 1 cut(s) 419
AjiI CACGTC 2 cut(s) 2170, 3137
AjuI GAANNNNNNNTTGG 4 cut(s) 1859, 1891, 2333, 2365
AloI GAACNNNNNNTCC 4 cut(s) 603, 635, 1024, 1056
Alw21I GWGCWC 4 cut(s) 1103, 1312, 3415, 4113
Alw26I GTCTC 3 cut(s) 642, 2453, 2668
Alw44I GTGCAC 1 cut(s) 1308
AlwNI CAGNNNCTG 4 cut(s) 1888, 2066, 2690, 3125
Ama87I CYCGRG 1 cut(s) 359
Aor51HI AGCGCT 1 cut(s) 4153
ApaLI GTGCAC 1 cut(s) 1308
ApoI RAATTY 6 cut(s) 594, 1240, 2374, 2420, 3499, 3852
AseI ATTAAT 1 cut(s) 1353
Asp700I GAANNNNTTC 2 cut(s) 1899, 3336
Asp718I GGTACC 2 cut(s) 3186, 3668
AspLEI GCGC 3 cut(s) 142, 3642, 4154
AsuC2I CCSGG 2 cut(s) 360, 361
AsuHPI GGTGA 5 cut(s) 971, 1324, 2198, 2738, 4214
AsuII TTCGAA 1 cut(s) 3698
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 6 cut(s) 34, 118, 868, 2254, 2386, 2822
AxyI CCTNAGG 2 cut(s) 2952, 3900
BaeGI GKGCMC 2 cut(s) 609, 1312
BaeI ACNNNNGTAYC 2 cut(s) 3660, 3693
BalI TGGCCA 1 cut(s) 3994
BamHI GGATCC 2 cut(s) 1015, 4137
BanI GGYRCC 3 cut(s) 3186, 3639, 3668
BanII GRGCYC 3 cut(s) 180, 1103, 4113
BauI CACGAG 1 cut(s) 4002
BbrPI CACGTG 1 cut(s) 3941
BbsI GAAGAC 3 cut(s) 266, 3080, 4082
Bbv12I GWGCWC 4 cut(s) 1103, 1312, 3415, 4113
BbvCI CCTCAGC 2 cut(s) 837, 3826
BceAI ACGGC 2 cut(s) 1439, 3495
BciVI GTATCC 1 cut(s) 2989
BclI TGATCA 1 cut(s) 3559
BcnI CCSGG 2 cut(s) 360, 361
BcoDI GTCTC 3 cut(s) 642, 2453, 2668
BfaI CTAG 4 cut(s) 797, 1734, 2021, 4211
BfmI CTRYAG 6 cut(s) 1889, 2058, 2151, 2276, 2691, 3810
BfoI RGCGCY 2 cut(s) 3643, 4155
BfuAI ACCTGC 1 cut(s) 22
BfuI GTATCC 1 cut(s) 2989
BglII AGATCT 1 cut(s) 2930
BmcAI AGTACT 1 cut(s) 1258
Bme18I GGWCC 6 cut(s) 34, 118, 868, 2254, 2386, 2822
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 2 cut(s) 2170, 3137
BmrI ACTGGG 3 cut(s) 175, 340, 2026
BmuI ACTGGG 3 cut(s) 175, 340, 2026
BoxI GACNNNNGTC 1 cut(s) 4086
BpiI GAAGAC 3 cut(s) 266, 3080, 4082
BpmI CTGGAG 4 cut(s) 666, 2435, 3193, 3936
Bpu10I CCTNAGC 5 cut(s) 240, 759, 837, 3611, 3826
Bpu14I TTCGAA 1 cut(s) 3698
BpuEI CTTGAG 6 cut(s) 1877, 2402, 2632, 2935, 3074, 3261
BpuMI CCSGG 2 cut(s) 360, 361
Bsa29I ATCGAT 1 cut(s) 3406
BsaAI YACGTR 1 cut(s) 3941
BsaBI GATNNNNATC 1 cut(s) 3777
BsaHI GRCGYC 2 cut(s) 2328, 3640
BsaJI CCNNGG 8 cut(s) 252, 359, 669, 2163, 2888, 2973, 3381, 3511
Bse21I CCTNAGG 2 cut(s) 2952, 3900
Bse3DI GCAATG 3 cut(s) 1415, 3348, 4141
Bse8I GATNNNNATC 1 cut(s) 3777
BseCI ATCGAT 1 cut(s) 3406
BseDI CCNNGG 8 cut(s) 252, 359, 669, 2163, 2888, 2973, 3381, 3511
BseJI GATNNNNATC 1 cut(s) 3777
BseMI GCAATG 3 cut(s) 1415, 3348, 4141
BseRI GAGGAG 2 cut(s) 1700, 3264
BseSI GKGCMC 2 cut(s) 609, 1312
BseYI CCCAGC 1 cut(s) 1433
BsgI GTGCAG 4 cut(s) 360, 498, 954, 1249
Bsh1236I CGCG 2 cut(s) 140, 254
BshNI GGYRCC 3 cut(s) 3186, 3639, 3668
BshVI ATCGAT 1 cut(s) 3406
BsiHKAI GWGCWC 4 cut(s) 1103, 1312, 3415, 4113
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 3 cut(s) 360, 1217, 4147
BslFI GGGAC 5 cut(s) 278, 342, 881, 910, 3743
BsmAI GTCTC 3 cut(s) 642, 2453, 2668
BsmFI GGGAC 5 cut(s) 278, 342, 881, 910, 3743
BsoBI CYCGRG 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 3698
Bsp1286I GDGCHC 6 cut(s) 180, 609, 1103, 1312, 3415, 4113
Bsp1407I TGTACA 1 cut(s) 422
BspDI ATCGAT 1 cut(s) 3406
BspFNI CGCG 2 cut(s) 140, 254
BspHI TCATGA 2 cut(s) 3367, 4093
BspMAI CTGCAG 2 cut(s) 1893, 2695
BspMI ACCTGC 1 cut(s) 22
BspQI GCTCTTC 3 cut(s) 303, 549, 3873
BspT104I TTCGAA 1 cut(s) 3698
BspT107I GGYRCC 3 cut(s) 3186, 3639, 3668
BsrDI GCAATG 3 cut(s) 1415, 3348, 4141
BsrGI TGTACA 1 cut(s) 422
BssECI CCNNGG 8 cut(s) 252, 359, 669, 2163, 2888, 2973, 3381, 3511
BssNI GRCGYC 2 cut(s) 2328, 3640
BssSI CACGAG 1 cut(s) 4002
BssT1I CCWWGG 2 cut(s) 2888, 3381
Bst2BI CACGAG 1 cut(s) 4002
Bst6I CTCTTC 6 cut(s) 303, 549, 731, 1126, 1770, 3873
BstACI GRCGYC 2 cut(s) 2328, 3640
BstAPI GCANNNNNTGC 3 cut(s) 977, 1991, 2690
BstAUI TGTACA 1 cut(s) 422
BstBAI YACGTR 1 cut(s) 3941
BstBI TTCGAA 1 cut(s) 3698
BstC8I GCNNGC 8 cut(s) 928, 1919, 2004, 2247, 3012, 3363, 3462, 4274
BstDSI CCRYGG 2 cut(s) 252, 3511
BstEII GGTNACC 1 cut(s) 2186
BstFNI CGCG 2 cut(s) 140, 254
BstH2I RGCGCY 2 cut(s) 3643, 4155
BstHHI GCGC 3 cut(s) 142, 3642, 4154
BstMAI GTCTC 3 cut(s) 642, 2453, 2668
BstNSI RCATGY 4 cut(s) 423, 1788, 3014, 3365
BstPAI GACNNNNGTC 1 cut(s) 4086
BstPI GGTNACC 1 cut(s) 2186
BstSFI CTRYAG 6 cut(s) 1889, 2058, 2151, 2276, 2691, 3810
BstSLI GKGCMC 2 cut(s) 609, 1312
BstUI CGCG 2 cut(s) 140, 254
BstV2I GAAGAC 3 cut(s) 266, 3080, 4082
BstX2I RGATCY 5 cut(s) 1015, 2930, 3895, 4098, 4137
BstYI RGATCY 5 cut(s) 1015, 2930, 3895, 4098, 4137
Bsu15I ATCGAT 1 cut(s) 3406
Bsu36I CCTNAGG 2 cut(s) 2952, 3900
BsuI GTATCC 1 cut(s) 2989
BsuTUI ATCGAT 1 cut(s) 3406
BtgI CCRYGG 2 cut(s) 252, 3511
BtgZI GCGATG 1 cut(s) 3764
BtrI CACGTC 2 cut(s) 2170, 3137
BtsI GCAGTG 4 cut(s) 1237, 2627, 3188, 3343
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 8 cut(s) 928, 1919, 2004, 2247, 3012, 3363, 3462, 4274
CaiI CAGNNNCTG 4 cut(s) 1888, 2066, 2690, 3125
CciI TCATGA 2 cut(s) 3367, 4093
CfoI GCGC 3 cut(s) 142, 3642, 4154
Cfr42I CCGCGG 1 cut(s) 255
Cfr9I CCCGGG 1 cut(s) 359
ClaI ATCGAT 1 cut(s) 3406
DinI GGCGCC 1 cut(s) 3641
DraIII CACNNNGTG 1 cut(s) 815
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 3992
Eam1104I CTCTTC 6 cut(s) 303, 549, 731, 1126, 1770, 3873
EarI CTCTTC 6 cut(s) 303, 549, 731, 1126, 1770, 3873
Ecl136II GAGCTC 2 cut(s) 1101, 4111
Eco130I CCWWGG 2 cut(s) 2888, 3381
Eco24I GRGCYC 3 cut(s) 180, 1103, 4113
Eco47I GGWCC 6 cut(s) 34, 118, 868, 2254, 2386, 2822
Eco47III AGCGCT 1 cut(s) 4153
Eco53kI GAGCTC 2 cut(s) 1101, 4111
Eco72I CACGTG 1 cut(s) 3941
Eco81I CCTNAGG 2 cut(s) 2952, 3900
Eco88I CYCGRG 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 2186
EcoICRI GAGCTC 2 cut(s) 1101, 4111
EcoO109I RGGNCCY 3 cut(s) 1324, 2161, 3470
EcoO65I GGTNACC 1 cut(s) 2186
EcoRI GAATTC 1 cut(s) 3852
EcoT14I CCWWGG 2 cut(s) 2888, 3381
EcoT22I ATGCAT 2 cut(s) 3363, 4030
EcoT38I GRGCYC 3 cut(s) 180, 1103, 4113
EgeI GGCGCC 1 cut(s) 3641
EheI GGCGCC 1 cut(s) 3641
ErhI CCWWGG 2 cut(s) 2888, 3381
FalI AAGNNNNNCTT 2 cut(s) 1901, 1933
FaqI GGGAC 5 cut(s) 278, 342, 881, 910, 3743
FauI CCCGC 2 cut(s) 106, 931
FauNDI CATATG 2 cut(s) 2368, 3319
FbaI TGATCA 1 cut(s) 3559
FblI GTMKAC 1 cut(s) 1024
FriOI GRGCYC 3 cut(s) 180, 1103, 4113
FspBI CTAG 4 cut(s) 797, 1734, 2021, 4211
GlaI GCGC 3 cut(s) 141, 3641, 4153
GsaI CCCAGC 1 cut(s) 1437
GsuI CTGGAG 4 cut(s) 666, 2435, 3193, 3936
HaeII RGCGCY 2 cut(s) 3643, 4155
HapII CCGG 3 cut(s) 360, 1217, 4147
HhaI GCGC 3 cut(s) 142, 3642, 4154
Hin1I GRCGYC 2 cut(s) 2328, 3640
Hin6I GCGC 3 cut(s) 140, 3640, 4152
HinP1I GCGC 3 cut(s) 140, 3640, 4152
HincII GTYRAC 2 cut(s) 136, 1636
HindII GTYRAC 2 cut(s) 136, 1636
HindIII AAGCTT 6 cut(s) 56, 408, 3207, 3458, 3693, 4010
HpaII CCGG 3 cut(s) 360, 1217, 4147
HphI GGTGA 5 cut(s) 971, 1324, 2198, 2738, 4214
HpyCH4IV ACGT 4 cut(s) 2169, 2328, 3136, 3940
HpySE526I ACGT 4 cut(s) 2169, 2328, 3136, 3940
Hsp92I GRCGYC 2 cut(s) 2328, 3640
HspAI GCGC 3 cut(s) 140, 3640, 4152
KasI GGCGCC 1 cut(s) 3639
KpnI GGTACC 2 cut(s) 3190, 3672
Ksp22I TGATCA 1 cut(s) 3559
KspI CCGCGG 1 cut(s) 255
LguI GCTCTTC 3 cut(s) 303, 549, 3873
MaeI CTAG 4 cut(s) 797, 1734, 2021, 4211
MaeII ACGT 4 cut(s) 2169, 2328, 3136, 3940
MfeI CAATTG 2 cut(s) 1766, 3160
MflI RGATCY 5 cut(s) 1015, 2930, 3895, 4098, 4137
MhlI GDGCHC 6 cut(s) 180, 609, 1103, 1312, 3415, 4113
MlsI TGGCCA 1 cut(s) 3994
MluNI TGGCCA 1 cut(s) 3994
Mly113I GGCGCC 1 cut(s) 3640
MlyI GAGTC 4 cut(s) 2239, 2438, 2455, 3433
MmeI TCCRAC 2 cut(s) 2325, 2331
Mox20I TGGCCA 1 cut(s) 3994
Mph1103I ATGCAT 2 cut(s) 3363, 4030
MroXI GAANNNNTTC 2 cut(s) 1899, 3336
MscI TGGCCA 1 cut(s) 3994
MslI CAYNNNNRTG 6 cut(s) 1467, 2800, 3241, 3366, 3408, 4200
Msp20I TGGCCA 1 cut(s) 3994
MspA1I CMGCKG 7 cut(s) 254, 617, 1433, 2048, 2063, 2573, 3070
MspI CCGG 3 cut(s) 360, 1217, 4147
MunI CAATTG 2 cut(s) 1766, 3160
MvnI CGCG 2 cut(s) 140, 254
NarI GGCGCC 1 cut(s) 3640
NciI CCSGG 2 cut(s) 360, 361
NdeI CATATG 2 cut(s) 2368, 3319
NmuCI GTSAC 9 cut(s) 49, 842, 1458, 1643, 2186, 2324, 2329, 3838, 4202
NsiI ATGCAT 2 cut(s) 3363, 4030
NspI RCATGY 4 cut(s) 423, 1788, 3014, 3365
NspV TTCGAA 1 cut(s) 3698
PaeI GCATGC 2 cut(s) 3014, 3365
PagI TCATGA 2 cut(s) 3367, 4093
PciI ACATGT 1 cut(s) 419
PciSI GCTCTTC 3 cut(s) 303, 549, 3873
PcsI WCGNNNNNNNCGW 1 cut(s) 4230
PdmI GAANNNNTTC 2 cut(s) 1899, 3336
PflFI GACNNNGTC 2 cut(s) 332, 653
PflMI CCANNNNNTGG 2 cut(s) 3084, 3125
PfoI TCCNGGA 1 cut(s) 4045
PleI GAGTC 4 cut(s) 2238, 2437, 2454, 3432
PluTI GGCGCC 1 cut(s) 3643
PmaCI CACGTG 1 cut(s) 3941
PmlI CACGTG 1 cut(s) 3941
PpsI GAGTC 4 cut(s) 2238, 2437, 2454, 3432
Ppu21I YACGTR 1 cut(s) 3941
PscI ACATGT 1 cut(s) 419
PshAI GACNNNNGTC 1 cut(s) 4086
PshBI ATTAAT 1 cut(s) 1353
Psp124BI GAGCTC 2 cut(s) 1103, 4113
PspCI CACGTG 1 cut(s) 3941
PspEI GGTNACC 1 cut(s) 2186
PspFI CCCAGC 1 cut(s) 1433
PstI CTGCAG 2 cut(s) 1893, 2695
PstNI CAGNNNCTG 4 cut(s) 1888, 2066, 2690, 3125
PsuI RGATCY 5 cut(s) 1015, 2930, 3895, 4098, 4137
PsyI GACNNNGTC 2 cut(s) 332, 653
PvuII CAGCTG 4 cut(s) 617, 1433, 2063, 2573
RseI CAYNNNNRTG 6 cut(s) 1467, 2800, 3241, 3366, 3408, 4200
SacI GAGCTC 2 cut(s) 1103, 4113
SacII CCGCGG 1 cut(s) 255
SapI GCTCTTC 3 cut(s) 303, 549, 3873
ScaI AGTACT 1 cut(s) 1258
SchI GAGTC 4 cut(s) 2239, 2438, 2455, 3433
SduI GDGCHC 6 cut(s) 180, 609, 1103, 1312, 3415, 4113
SfcI CTRYAG 6 cut(s) 1889, 2058, 2151, 2276, 2691, 3810
SfoI GGCGCC 1 cut(s) 3641
Sfr303I CCGCGG 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 3698
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 6 cut(s) 34, 118, 868, 2254, 2386, 2822
SmaI CCCGGG 1 cut(s) 361
SmiMI CAYNNNNRTG 6 cut(s) 1467, 2800, 3241, 3366, 3408, 4200
SmlI CTYRAG 6 cut(s) 1856, 2381, 2611, 2914, 3053, 3276
SmoI CTYRAG 6 cut(s) 1856, 2381, 2611, 2914, 3053, 3276
SphI GCATGC 2 cut(s) 3014, 3365
SspDI GGCGCC 1 cut(s) 3639
SspI AATATT 3 cut(s) 3556, 3725, 3881
SspMI CTAG 4 cut(s) 797, 1734, 2021, 4211
SstI GAGCTC 2 cut(s) 1103, 4113
StyI CCWWGG 2 cut(s) 2888, 3381
TaiI ACGT 4 cut(s) 2172, 2331, 3139, 3943
TaqI TCGA 5 cut(s) 2126, 2141, 3406, 3698, 4233
TaqII GACCGA 1 cut(s) 2242
TatI WGTACW 5 cut(s) 422, 910, 1256, 2587, 2864
TauI GCSGC 4 cut(s) 1507, 1510, 1513, 3819
TseFI GTSAC 9 cut(s) 49, 842, 1458, 1643, 2186, 2324, 2329, 3838, 4202
Tsp45I GTSAC 9 cut(s) 49, 842, 1458, 1643, 2186, 2324, 2329, 3838, 4202
TspGWI ACGGA 6 cut(s) 624, 1640, 1888, 2861, 3256, 3275
TspMI CCCGGG 1 cut(s) 359
Tth111I GACNNNGTC 2 cut(s) 332, 653
Van91I CCANNNNNTGG 2 cut(s) 3084, 3125
VneI GTGCAC 1 cut(s) 1308
VpaK11BI GGWCC 6 cut(s) 34, 118, 868, 2254, 2386, 2822
VspI ATTAAT 1 cut(s) 1353
XapI RAATTY 6 cut(s) 594, 1240, 2374, 2420, 3499, 3852
XceI RCATGY 4 cut(s) 423, 1788, 3014, 3365
XcmI CCANNNNNNNNNTGG 1 cut(s) 3435
XmaI CCCGGG 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 1024
XmnI GAANNNNTTC 2 cut(s) 1899, 3336
XspI CTAG 4 cut(s) 797, 1734, 2021, 4211
ZraI GACGTC 1 cut(s) 2329
ZrmI AGTACT 1 cut(s) 1258
Zsp2I ATGCAT 2 cut(s) 3363, 4030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.