Rh2AG584800

Alpha-amylase C-terminal beta-sheet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003013
Physical Location & Seq
Forward (+)
81787745 .. 81795740
7996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG584800.1

Sequence Viewer

Length: 2697 bp
ATGGGTGTTATTTCACTGCCTGATATGGCCAACCTCGCCGTGTCATATTCAGGTGGACATTGCAAACATGATACGACTTTATTCCTGCGGCGGCGCCCTCTTATTTTCAGAACAAACTCGAATAGAAAAAAGAAACTATCCCTCACAGAACATTGGCTATGTAAATCAAGAACAAGCATATTTTCAAGCCTGGATGATTCCAGTGTTACAGATGTTGTTGACAACAATGACAGTTTTTCTTCAGGAAGAAATGAAATGTTGAGTGTAGAGGAGGATGAGTTAATGACAGCTAAATTAGCACTTTCTGAGGCTCATGCTAGTCAAGAAGCCATTGAGAAAGAGAGGGATCAATTGCTTGAAGAGTTGGCATGTTTTGAGGCAAAACAAAAGGAATATGTAGCTACCATATTGCATGACAAGGATATGGCTGTTTCGGAACTTGAGGCTGCAAAATCTTTGTTCAATCAGAAGCTGCAGGAATCAGTTCAAGAGAAGTTCAGCTTGCAAGATAAGTTAGTCCTTGTGAAACAAGATGCTGTTCAACTTGCGGTACAAGTAGAGAGGCTAGCAGAAATTGCTTTTCAGCAGGCAACATCTCACATACTAGAAGATGCCCAGTTGAGGGTTTCCGCTGCTGAAACTACAGCTGCTGAAGCATCTTATCAGATAGAAAAGCAAATTAAGGATATGACTGAAGGTACAATATTGTCGATTGTGGAACAGTCGAAAAATGCTATAGAGAAGGCCCTGGACGTGGCAGAAAAAGCTGGTGATCAGGCATCAAAAGCTGCGTCAGCATTTAATGACAGTATGAGTCCACTTGATGAGCTTGCTTCGGTCCAGTCAAAGAACATCATGCTACAGGGTGCTGTGAATGATTTAGAATCTCAGTTATTGCTTACAAAAAGTGACGTCGCTAAGTTGAAGTTGGAGTTGGAAAAGGCCCATGCATATGCAAATTTGCTTGAGGTCCGAGCTACTGATGCTGAGAAAGCATTGGTTGAATTTCAGGAGTCAAGCAGCAAAGAGAGTCTCCAGAGAGAGCAGGAAATCATGTCATTGATGGAGCAGATGAAGAAAGATTCATCGGAAAGAAACCAGGCTGCTTCAGGGGCTTTCAATGTTGAGTTACAAAGCATTAGGGATGCTGTTGCAGCTGTGAAAGAAACAGTACATTCCAAAGATGATGCCTACTTGAGAAGATGCGAAGCACTGCAAAGATCGTTGAAGGCATCTGAAGCTACTACAAAGATGTGGAGACAGAGAGCAGAAACTGCAGAATCCTTGTTATTGAAGGAAAGGCAGCCAGGTGATCGGGAAGAAGATTCCATTTTTGTTGTTAATGGTGGAAGGATAGACCTTTTGACCAATGATGATTCACAGAAATGGAAACTTTTAAGTGATGGTCCTCGCAGAGAGATACCTCAATGGATGGCAAGGAGAATATGTACCATCCGTACCAAGTTTCCTCCAAGGAAGATTGATGTAGCTGAAGCCTTGAGTTCAGAGTTCAGATCTTTGAACTTGCCAAAGCCTGAGGAAGTATGGTCAATAGCTCTGGAAAAGCCAAAGGAGGGGGATACACTTATTGAGCATGCTTTTGAGAAAGAAACAATAGAGAAGAAAAGGAAGGCTCTTGAGCGTGTTCTTCAGCGGAAGACCACACAATGGCAGAGGACTGAGGAACAAACAAAATTAGAGCCAGGAACTGGAACAGGACGTGAGATTGTGTTTCAAGGTTTCAATTGGGAAAGCTGGAGAAAGCAGTGGTACCTTGATTTAGCTCCTAAAGCTTCTGATTTATCTAAAATTGGGGTAACATCAGTGTGGTTCCCACCACCAACGGAATCCGTTGCTCCTCAAGGTTATATGCCTTCTGACCTTTACAATTTGAACTCGGCATATGGTACTAAGGAAGAACTTCAACACTGCATTGCGGAGATGCATGCTCATGATCTTCTGGCCTTGGGAGATGTTGTCCTGAATCATCGATGTGCTCATAAACAGAGTCCAAATGGTGTTTGGAACATCTTTGGTGGGAAGCTTGCATGGGGGCCTGAAGCAATCGTTTGTGATGATCCAAATTTTGAGGGCCGTGGAAACCCTTCGAGTGGGGATATATTCCATGCAGCACCCAATATTGATCATTCAAAGGACTTTGTAAGAAAAGATATAAAGGACTGGCTAAACTGGCTTAGGAGTGATATTGGTTTTGATGGATGGCGCCTTGATTTTGTAAGAGGCTTCTCTGGTACCCATGTAAAGGAATATATTGAAGCTTCGAATCCAGCATTTGCTATTGGAGAATATTGGGACAGTTTAGCTTATGAAAATGGAAGTTTGTGTTACAATCAAGATGCTCATCGCCAAAGGATAGTTAATTGGATCAATGCTTCAGGCGGCAGTTCCTCAGCATTTGATGTCACAACTAAGGGAATTCTCCATTGTGCTCTTCATAACCAATATTGGAGGTTAATAGATCCTCAGGGTAAACCAACTGGAGTTATGGGATGGTGGCCCTCACGTGCTGTTACATTTTTGGAGAACCATGACACAGGATCAACACAGGGTCATTGGCCATTTCCACGAGAAAAGCTTACACAGGGATATGCATACATTTTGACCCATCCTGGAACAGTATATTCTCAATCACCCAATTGCCAATCTGCTTTCTTTTTCTCTTTGGGGGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

898

Amino Acids

101.11

Weight (kDa)

5.46

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 601 - 665 8.6e-09 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 915
Acc65I GGTACC 2 cut(s) 1770, 2252
AccB1I GGYRCC 4 cut(s) 93, 1770, 2223, 2252
AccB7I CCANNNNNTGG 2 cut(s) 1668, 1709
AciI CCGC 7 cut(s) 88, 91, 548, 630, 1654, 1937, 2400
AclWI GGATC 5 cut(s) 354, 2072, 2393, 2474, 2566
AcoI YGGCCR 2 cut(s) 27, 2576
AcsI RAATTY 4 cut(s) 958, 1004, 2083, 2436
AcuI CTGAAG 9 cut(s) 225, 672, 714, 1092, 1257, 1512, 1634, 2079, 2379
AcvI CACGTG 1 cut(s) 2525
AcyI GRCGYC 3 cut(s) 94, 912, 2224
AfaI GTAC 8 cut(s) 552, 700, 1173, 1450, 1459, 1772, 1909, 2254
AfiI CCNNNNNNNGG 8 cut(s) 621, 622, 754, 1574, 1668, 1709, 2111, 2263
AjiI CACGTC 2 cut(s) 754, 1721
AjnI CCWGG 6 cut(s) 189, 747, 1098, 1306, 1702, 2629
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
Alw21I GWGCWC 2 cut(s) 1999, 2452
Alw26I GTCTC 2 cut(s) 1037, 1252
AlwI GGATC 5 cut(s) 354, 2072, 2393, 2474, 2566
AlwNI CAGNNNCTG 4 cut(s) 472, 650, 1274, 1709
AoxI GGCC 8 cut(s) 27, 744, 942, 1962, 2054, 2092, 2516, 2576
ApoI RAATTY 4 cut(s) 958, 1004, 2083, 2436
ArsI GACNNNNNNTTYG 2 cut(s) 776, 808
Asp700I GAANNNNTTC 3 cut(s) 483, 1920, 2104
Asp718I GGTACC 2 cut(s) 1770, 2252
AspLEI GCGC 2 cut(s) 96, 2226
AspS9I GGNCC 8 cut(s) 745, 838, 943, 970, 1406, 2054, 2092, 2517
AsuHPI GGTGA 3 cut(s) 782, 1322, 2643
AsuII TTCGAA 1 cut(s) 2282
AsuNHI GCTAGC 1 cut(s) 565
AvaII GGWCC 3 cut(s) 838, 970, 1406
AxyI CCTNAGG 2 cut(s) 1536, 2484
BaeI ACNNNNGTAYC 2 cut(s) 2244, 2277
BalI TGGCCA 2 cut(s) 29, 2578
BanI GGYRCC 4 cut(s) 93, 1770, 2223, 2252
BauI CACGAG 1 cut(s) 2586
BbrPI CACGTG 1 cut(s) 2525
BbsI GAAGAC 1 cut(s) 1664
Bbv12I GWGCWC 2 cut(s) 1999, 2452
BbvCI CCTCAGC 1 cut(s) 2410
BccI CCATC 8 cut(s) 1057, 1397, 1426, 1460, 2210, 2214, 2505, 2634
BceAI ACGGC 2 cut(s) 23, 2079
BciT130I CCWGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
BciVI GTATCC 1 cut(s) 1573
BclI TGATCA 2 cut(s) 772, 2143
BcoDI GTCTC 2 cut(s) 1037, 1252
BfaI CTAG 3 cut(s) 318, 566, 605
BfmI CTRYAG 5 cut(s) 473, 642, 735, 860, 1275
BfoI RGCGCY 2 cut(s) 97, 2227
BfuI GTATCC 1 cut(s) 1573
BglII AGATCT 1 cut(s) 1514
Bme1390I CCNGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
Bme18I GGWCC 3 cut(s) 838, 970, 1406
BmgBI CACGTC 2 cut(s) 754, 1721
BmgT120I GGNCC 8 cut(s) 745, 838, 943, 970, 1406, 2054, 2092, 2517
BmiI GGNNCC 6 cut(s) 95, 1772, 1832, 2055, 2225, 2254
BmrFI CCNGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
BmrI ACTGGG 1 cut(s) 610
BmtI GCTAGC 1 cut(s) 569
BmuI ACTGGG 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 1664
BpmI CTGGAG 3 cut(s) 1019, 1777, 2520
Bpu10I CCTNAGC 2 cut(s) 2195, 2410
Bpu14I TTCGAA 1 cut(s) 2282
BpuEI CTTGAG 6 cut(s) 461, 986, 1216, 1519, 1658, 1845
Bsa29I ATCGAT 1 cut(s) 1990
BsaAI YACGTR 1 cut(s) 2525
BsaBI GATNNNNATC 1 cut(s) 2361
BsaHI GRCGYC 3 cut(s) 94, 912, 2224
BsaJI CCNNGG 4 cut(s) 747, 1472, 1965, 2095
BsaXI ACNNNNNCTCC 6 cut(s) 263, 293, 1432, 1462, 1962, 1992
Bsc4I CCNNNNNNNGG 8 cut(s) 621, 622, 754, 1574, 1668, 1709, 2111, 2263
Bse1I ACTGG 7 cut(s) 201, 616, 841, 1714, 2186, 2195, 2503
Bse21I CCTNAGG 2 cut(s) 1536, 2484
Bse3DI GCAATG 2 cut(s) 58, 1932
Bse8I GATNNNNATC 1 cut(s) 2361
BseBI CCWGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
BseCI ATCGAT 1 cut(s) 1990
BseDI CCNNGG 4 cut(s) 747, 1472, 1965, 2095
BseGI GGATG 9 cut(s) 199, 280, 1150, 1437, 1452, 2225, 2516, 2626, 2695
BseJI GATNNNNATC 1 cut(s) 2361
BseLI CCNNNNNNNGG 8 cut(s) 621, 622, 754, 1574, 1668, 1709, 2111, 2263
BseMI GCAATG 2 cut(s) 58, 1932
BseMII CTCAG 7 cut(s) 297, 902, 978, 1527, 1671, 2424, 2498
BseNI ACTGG 7 cut(s) 201, 616, 841, 1714, 2186, 2195, 2503
BseRI GAGGAG 2 cut(s) 284, 1848
BshFI GGCC 8 cut(s) 29, 746, 944, 1964, 2056, 2094, 2518, 2578
BshNI GGYRCC 4 cut(s) 93, 1770, 2223, 2252
BshVI ATCGAT 1 cut(s) 1990
BsiHKAI GWGCWC 2 cut(s) 1999, 2452
BslFI GGGAC 1 cut(s) 2327
BslI CCNNNNNNNGG 8 cut(s) 621, 622, 754, 1574, 1668, 1709, 2111, 2263
BsmAI GTCTC 2 cut(s) 1037, 1252
BsmFI GGGAC 1 cut(s) 2327
BsnI GGCC 8 cut(s) 29, 746, 944, 1964, 2056, 2094, 2518, 2578
Bsp119I TTCGAA 1 cut(s) 2282
Bsp1286I GDGCHC 2 cut(s) 1999, 2452
BspACI CCGC 7 cut(s) 88, 91, 548, 630, 1654, 1937, 2400
BspANI GGCC 8 cut(s) 29, 746, 944, 1964, 2056, 2094, 2518, 2578
BspCNI CTCAG 7 cut(s) 298, 901, 979, 1528, 1672, 2423, 2497
BspDI ATCGAT 1 cut(s) 1990
BspHI TCATGA 1 cut(s) 1951
BspLI GGNNCC 6 cut(s) 95, 1772, 1832, 2055, 2225, 2254
BspMAI CTGCAG 2 cut(s) 477, 1279
BspOI GCTAGC 1 cut(s) 569
BspPI GGATC 5 cut(s) 354, 2072, 2393, 2474, 2566
BspQI GCTCTTC 1 cut(s) 2457
BspT104I TTCGAA 1 cut(s) 2282
BspT107I GGYRCC 4 cut(s) 93, 1770, 2223, 2252
BsrDI GCAATG 2 cut(s) 58, 1932
BsrI ACTGG 7 cut(s) 201, 616, 841, 1714, 2186, 2195, 2503
BssECI CCNNGG 4 cut(s) 747, 1472, 1965, 2095
BssNI GRCGYC 3 cut(s) 94, 912, 2224
BssSI CACGAG 1 cut(s) 2586
BssT1I CCWWGG 2 cut(s) 1472, 1965
Bst2BI CACGAG 1 cut(s) 2586
Bst2UI CCWGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
Bst4CI ACNGT 6 cut(s) 233, 723, 809, 1171, 2318, 2638
Bst6I CTCTTC 2 cut(s) 354, 2457
BstACI GRCGYC 3 cut(s) 94, 912, 2224
BstAPI GCANNNNNTGC 2 cut(s) 575, 1274
BstBAI YACGTR 1 cut(s) 2525
BstBI TTCGAA 1 cut(s) 2282
BstC8I GCNNGC 7 cut(s) 503, 567, 588, 831, 1596, 1947, 2046
BstDSI CCRYGG 1 cut(s) 2095
BstF5I GGATG 9 cut(s) 199, 280, 1150, 1437, 1452, 2225, 2516, 2626, 2695
BstH2I RGCGCY 2 cut(s) 97, 2227
BstHHI GCGC 2 cut(s) 96, 2226
BstMAI GTCTC 2 cut(s) 1037, 1252
BstNI CCWGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
BstNSI RCATGY 3 cut(s) 372, 1598, 1949
BstSCI CCNGG 6 cut(s) 189, 747, 1098, 1306, 1702, 2629
BstSFI CTRYAG 5 cut(s) 473, 642, 735, 860, 1275
BstV2I GAAGAC 1 cut(s) 1664
BstX2I RGATCY 2 cut(s) 1514, 2479
BstYI RGATCY 2 cut(s) 1514, 2479
Bsu15I ATCGAT 1 cut(s) 1990
Bsu36I CCTNAGG 2 cut(s) 1536, 2484
BsuI GTATCC 1 cut(s) 1573
BsuRI GGCC 8 cut(s) 29, 746, 944, 1964, 2056, 2094, 2518, 2578
BsuTUI ATCGAT 1 cut(s) 1990
BtgI CCRYGG 1 cut(s) 2095
BtgZI GCGATG 1 cut(s) 2348
BtrI CACGTC 2 cut(s) 754, 1721
BtsCI GGATG 9 cut(s) 199, 280, 1150, 1437, 1452, 2225, 2516, 2626, 2695
BtsI GCAGTG 4 cut(s) 14, 1211, 1772, 1927
BtsIMutI CAGTG 6 cut(s) 14, 208, 1211, 1772, 1830, 1927
Cac8I GCNNGC 7 cut(s) 503, 567, 588, 831, 1596, 1947, 2046
CaiI CAGNNNCTG 4 cut(s) 472, 650, 1274, 1709
CciI TCATGA 1 cut(s) 1951
CfoI GCGC 2 cut(s) 96, 2226
Cfr13I GGNCC 8 cut(s) 745, 838, 943, 970, 1406, 2054, 2092, 2517
ClaI ATCGAT 1 cut(s) 1990
CseI GACGC 1 cut(s) 780
Csp6I GTAC 8 cut(s) 551, 699, 1172, 1449, 1458, 1771, 1908, 2253
CviQI GTAC 8 cut(s) 551, 699, 1172, 1449, 1458, 1771, 1908, 2253
DinI GGCGCC 2 cut(s) 95, 2225
EaeI YGGCCR 2 cut(s) 27, 2576
Eam1104I CTCTTC 2 cut(s) 354, 2457
EarI CTCTTC 2 cut(s) 354, 2457
Eco130I CCWWGG 2 cut(s) 1472, 1965
Eco47I GGWCC 3 cut(s) 838, 970, 1406
Eco57I CTGAAG 9 cut(s) 225, 672, 714, 1092, 1257, 1512, 1634, 2079, 2379
Eco72I CACGTG 1 cut(s) 2525
Eco81I CCTNAGG 2 cut(s) 1536, 2484
EcoO109I RGGNCCY 2 cut(s) 745, 2054
EcoRI GAATTC 1 cut(s) 2436
EcoRII CCWGG 6 cut(s) 189, 747, 1098, 1306, 1702, 2629
EcoT14I CCWWGG 2 cut(s) 1472, 1965
EcoT22I ATGCAT 3 cut(s) 952, 1947, 2614
EgeI GGCGCC 2 cut(s) 95, 2225
EheI GGCGCC 2 cut(s) 95, 2225
ErhI CCWWGG 2 cut(s) 1472, 1965
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 1 cut(s) 2327
FauNDI CATATG 2 cut(s) 952, 1903
FbaI TGATCA 2 cut(s) 772, 2143
FokI GGATG 8 cut(s) 206, 287, 1157, 1439, 1444, 2232, 2523, 2613
FspBI CTAG 3 cut(s) 318, 566, 605
GlaI GCGC 2 cut(s) 95, 2225
GsuI CTGGAG 3 cut(s) 1019, 1777, 2520
HaeII RGCGCY 2 cut(s) 97, 2227
HaeIII GGCC 8 cut(s) 29, 746, 944, 1964, 2056, 2094, 2518, 2578
HgaI GACGC 1 cut(s) 780
HhaI GCGC 2 cut(s) 96, 2226
Hin1I GRCGYC 3 cut(s) 94, 912, 2224
Hin6I GCGC 2 cut(s) 94, 2224
HinP1I GCGC 2 cut(s) 94, 2224
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 4 cut(s) 1791, 2042, 2277, 2594
HphI GGTGA 3 cut(s) 782, 1322, 2643
Hpy166II GTNNAC 4 cut(s) 56, 220, 818, 2492
Hpy8I GTNNAC 4 cut(s) 56, 220, 818, 2492
Hpy99I CGWCG 1 cut(s) 917
HpyAV CCTTC 8 cut(s) 689, 736, 1222, 1288, 1344, 1624, 1884, 2115
HpyCH4III ACNGT 6 cut(s) 233, 723, 809, 1171, 2318, 2638
HpyCH4IV ACGT 4 cut(s) 753, 912, 1720, 2524
HpySE526I ACGT 4 cut(s) 753, 912, 1720, 2524
Hsp92I GRCGYC 3 cut(s) 94, 912, 2224
HspAI GCGC 2 cut(s) 94, 2224
KasI GGCGCC 2 cut(s) 93, 2223
KpnI GGTACC 2 cut(s) 1774, 2256
Ksp22I TGATCA 2 cut(s) 772, 2143
LguI GCTCTTC 1 cut(s) 2457
LmnI GCTCC 3 cut(s) 1066, 1789, 1861
MaeI CTAG 3 cut(s) 318, 566, 605
MaeII ACGT 4 cut(s) 753, 912, 1720, 2524
MaeIII GTNAC 7 cut(s) 205, 908, 1128, 1816, 2345, 2422, 2530
MfeI CAATTG 3 cut(s) 350, 1744, 2656
MflI RGATCY 2 cut(s) 1514, 2479
MhlI GDGCHC 2 cut(s) 1999, 2452
MlsI TGGCCA 2 cut(s) 29, 2578
MluNI TGGCCA 2 cut(s) 29, 2578
Mly113I GGCGCC 2 cut(s) 94, 2224
MlyI GAGTC 4 cut(s) 823, 1022, 1039, 2017
MmeI TCCRAC 2 cut(s) 909, 915
Mox20I TGGCCA 2 cut(s) 29, 2578
Mph1103I ATGCAT 3 cut(s) 952, 1947, 2614
MroXI GAANNNNTTC 3 cut(s) 483, 1920, 2104
MscI TGGCCA 2 cut(s) 29, 2578
MseI TTAA 7 cut(s) 281, 681, 801, 1341, 1397, 2379, 2474
MslI CAYNNNNRTG 5 cut(s) 951, 1384, 1825, 1950, 1992
Msp20I TGGCCA 2 cut(s) 29, 2578
MspA1I CMGCKG 4 cut(s) 632, 647, 1157, 1654
MspR9I CCNGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
MunI CAATTG 3 cut(s) 350, 1744, 2656
MvaI CCWGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
NarI GGCGCC 2 cut(s) 94, 2224
NdeI CATATG 2 cut(s) 952, 1903
NheI GCTAGC 1 cut(s) 565
NlaIV GGNNCC 6 cut(s) 95, 1772, 1832, 2055, 2225, 2254
NmeAIII GCCGAG 1 cut(s) 1877
NmuCI GTSAC 2 cut(s) 908, 2422
NsiI ATGCAT 3 cut(s) 952, 1947, 2614
NspI RCATGY 3 cut(s) 372, 1598, 1949
NspV TTCGAA 1 cut(s) 2282
PaeI GCATGC 2 cut(s) 1598, 1949
PagI TCATGA 1 cut(s) 1951
PciSI GCTCTTC 1 cut(s) 2457
PdmI GAANNNNTTC 3 cut(s) 483, 1920, 2104
PflMI CCANNNNNTGG 2 cut(s) 1668, 1709
PfoI TCCNGGA 1 cut(s) 2629
PleI GAGTC 4 cut(s) 822, 1021, 1038, 2016
PluTI GGCGCC 2 cut(s) 97, 2227
PmaCI CACGTG 1 cut(s) 2525
PmlI CACGTG 1 cut(s) 2525
PpsI GAGTC 4 cut(s) 822, 1021, 1038, 2016
Ppu21I YACGTR 1 cut(s) 2525
Psp6I CCWGG 6 cut(s) 189, 747, 1098, 1306, 1702, 2629
PspCI CACGTG 1 cut(s) 2525
PspGI CCWGG 6 cut(s) 189, 747, 1098, 1306, 1702, 2629
PspN4I GGNNCC 6 cut(s) 95, 1772, 1832, 2055, 2225, 2254
PspPI GGNCC 8 cut(s) 745, 838, 943, 970, 1406, 2054, 2092, 2517
PstI CTGCAG 2 cut(s) 477, 1279
PstNI CAGNNNCTG 4 cut(s) 472, 650, 1274, 1709
PsuI RGATCY 2 cut(s) 1514, 2479
PvuII CAGCTG 2 cut(s) 647, 1157
RsaI GTAC 8 cut(s) 552, 700, 1173, 1450, 1459, 1772, 1909, 2254
RsaNI GTAC 8 cut(s) 551, 699, 1172, 1449, 1458, 1771, 1908, 2253
RseI CAYNNNNRTG 5 cut(s) 951, 1384, 1825, 1950, 1992
SapI GCTCTTC 1 cut(s) 2457
SaqAI TTAA 7 cut(s) 281, 681, 801, 1341, 1397, 2379, 2474
Sau96I GGNCC 8 cut(s) 745, 838, 943, 970, 1406, 2054, 2092, 2517
SchI GAGTC 4 cut(s) 823, 1022, 1039, 2017
ScrFI CCNGG 6 cut(s) 191, 749, 1100, 1308, 1704, 2631
SduI GDGCHC 2 cut(s) 1999, 2452
SfcI CTRYAG 5 cut(s) 473, 642, 735, 860, 1275
SfoI GGCGCC 2 cut(s) 95, 2225
SfuI TTCGAA 1 cut(s) 2282
SinI GGWCC 3 cut(s) 838, 970, 1406
SmiMI CAYNNNNRTG 5 cut(s) 951, 1384, 1825, 1950, 1992
SmlI CTYRAG 6 cut(s) 440, 965, 1195, 1498, 1637, 1860
SmoI CTYRAG 6 cut(s) 440, 965, 1195, 1498, 1637, 1860
SphI GCATGC 2 cut(s) 1598, 1949
SsiI CCGC 7 cut(s) 88, 91, 548, 630, 1654, 1937, 2400
SspDI GGCGCC 2 cut(s) 93, 2223
SspI AATATT 4 cut(s) 705, 2140, 2309, 2465
SspMI CTAG 3 cut(s) 318, 566, 605
StyD4I CCNGG 6 cut(s) 189, 747, 1098, 1306, 1702, 2629
StyI CCWWGG 2 cut(s) 1472, 1965
TaaI ACNGT 6 cut(s) 233, 723, 809, 1171, 2318, 2638
TaiI ACGT 4 cut(s) 756, 915, 1723, 2527
TaqI TCGA 6 cut(s) 119, 710, 725, 1990, 2108, 2282
TaqII GACCGA 1 cut(s) 826
TatI WGTACW 1 cut(s) 1171
TauI GCSGC 3 cut(s) 91, 94, 2403
Tru1I TTAA 7 cut(s) 281, 681, 801, 1341, 1397, 2379, 2474
Tru9I TTAA 7 cut(s) 281, 681, 801, 1341, 1397, 2379, 2474
TscAI CASTG 6 cut(s) 21, 208, 1218, 1772, 1830, 1934
TseFI GTSAC 2 cut(s) 908, 2422
Tsp45I GTSAC 2 cut(s) 908, 2422
TspDTI ATGAA 5 cut(s) 267, 1074, 1088, 2343, 2444
TspGWI ACGGA 3 cut(s) 1445, 1840, 1859
TspRI CASTG 6 cut(s) 21, 208, 1218, 1772, 1830, 1934
Van91I CCANNNNNTGG 2 cut(s) 1668, 1709
VpaK11BI GGWCC 3 cut(s) 838, 970, 1406
XapI RAATTY 4 cut(s) 958, 1004, 2083, 2436
XceI RCATGY 3 cut(s) 372, 1598, 1949
XcmI CCANNNNNNNNNTGG 1 cut(s) 2019
XmnI GAANNNNTTC 3 cut(s) 483, 1920, 2104
XspI CTAG 3 cut(s) 318, 566, 605
ZraI GACGTC 1 cut(s) 913
Zsp2I ATGCAT 3 cut(s) 952, 1947, 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.