Rw2G048750

Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003013
Physical Location & Seq
Forward (+)
76313440 .. 76317144
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G048750.1

Sequence Viewer

Length: 1299 bp
ATGGAGATAGAGGAAGGCACACATGACAGGCAGGTCCAGGTTCGCTTCGTCACGAAGCTTCAGGACATCCCCTTCAAAGTTCCCAACACCACCATTCCCGCCTCCTATTCCCGTTTGGACCTCTCCAAAGTCGTCAACGCTCTCATCCAAACCGCAAATCCTGACTGGGAGTCGGAGCCCTTTGATTTTCTCATCAATGGCGTATTGGTTCGGATGTCGCTTGGACAGTTCCTTCTTGCTAAGGGCATTTCCGCGGAGAAGACATTGGAAGTTGAATATATACGGGCTGTTGTCCCACGCAGAGAAGAAGAGCCTTGTTTACATGATGACTGGGTCGGTGCAGTTGATGGTTCCAGTCCTGGGTTCATTTTGACAGGGTGCTATGATGGTTTAGGAAGGGTATGGAAAGCTTCTGGAACATGTACACATATACTGCAAGGACACAGTGAACCAGTTACATCTGTTAGTGTTATCAGTGCAGAAGGGGGAGAAAGTTGTAGCGTAGCTACTGCTTCAAAAGATCGCACACTGAGGCTGTGGAAGTTCAATACAGAAGAGCCAACAAACAATCCTTTGAGGACAACAGCATACAAAATTTTGCGTGGGCACGGAGCAGCTGTTCAAAGTGTCGCTGCTCATACCTCCGGAGACATGGTCTGTTCAGGCTCCTGGGATTCCACAATCAAATTATGGCAGACAGATGAGCCTGATATAGAAGTTGATGTGTCAATTAAGAAGAGAAAAAAAATTGATGAAGCTAAGGAATCTCAAAAGGAGGGGGAGGCTGTTTCTTCTCTAGTGGGCCATACACAATGTGTATCTTCTGTCAAGTGGCCTCAGCGTGACATAATCTATTCGGCATCTTGGGACCATTCTATTAGAAGGTGGGATGTTGGGACAGGCAAAGATGTACTGAACATACCCGCCAGCAAAGCCCTCAACTGTCTTGATATTGGTGGTGAAGGTTCTGCACTTGTTGCTGCTGGTGGTTCTGACCCCATTCTTAGGATATGGGATCCTCGTAGACCAGGAACTTCTGCTCCTGTTGGTCAATTCTCATCTCACACTTCTTGGATTACGGGATGCAAGTGGCATGAGAGCTCTTGGTTTCATATACTCTCTTCATCGTATGATGGCAAAGTCATGCTATGGGATATGAGAACTACTTGGCCTTTATTTGTCATTGATTCACATAAAGACAGCAAGGTACTATGTGTGGATTGGTGGAAAGGTGATAGTGTGGTTAGTGGTGGGGCAGATACAAAGCTTTGCATCAGTTCCGGCGTTTCTGTGCAGTGA

Protein Analysis

432

Amino Acids

47.43

Weight (kDa)

5.77

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NLE PF08154 12 - 78 7.5e-15 NLE (NUC135) domain
Beta-prop_THOC3 PF25174 108 - 190 1.5e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 116 - 238 2.1e-12 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 119 - 237 1.4e-08 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 120 - 235 2.5e-16 WDR5 beta-propeller domain
WD40 PF00400 139 - 180 7e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 149 - 311 9.9e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 168 - 238 2.1e-10 THOC3 beta-propeller domain
WD40 PF00400 198 - 231 7.2e-08 WD domain, G-beta repeat
WDR55 PF24796 256 - 423 2e-08 WDR55
Beta-prop_WDR5 PF25175 258 - 341 2.3e-10 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 259 - 421 1.3e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 260 - 311 1.9e-06 THOC3 beta-propeller domain
WD40_Gbeta PF25391 265 - 420 2.8e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 279 - 415 1.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 311 - 422 1.2e-06 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 312 - 425 2.4e-10 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 312 - 422 2.5e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 314 - 423 1.4e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 323 - 423 1.8e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
Acc36I ACCTGC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 1024
AccII CGCG 1 cut(s) 254
AccIII TCCGGA 1 cut(s) 644
AciI CCGC 5 cut(s) 99, 153, 252, 254, 924
AclWI GGATC 2 cut(s) 1010, 1023
AcsI RAATTY 1 cut(s) 594
AcuI CTGAAG 1 cut(s) 44
AdeI CACNNNGTG 1 cut(s) 815
AfaI GTAC 3 cut(s) 424, 912, 1209
AfiI CCNNNNNNNGG 2 cut(s) 360, 1005
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 5 cut(s) 76, 275, 516, 547, 623
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 36, 358, 668, 1027
AloI GAACNNNNNNTCC 4 cut(s) 603, 635, 1024, 1056
AluBI AGCT 7 cut(s) 58, 410, 506, 617, 758, 1101, 1267
AluI AGCT 7 cut(s) 58, 410, 506, 617, 758, 1101, 1267
Alw21I GWGCWC 1 cut(s) 1103
Alw26I GTCTC 1 cut(s) 642
AlwI GGATC 2 cut(s) 1010, 1023
Aor13HI TCCGGA 1 cut(s) 644
AoxI GGCC 3 cut(s) 802, 833, 1169
ApeKI GCWGC 3 cut(s) 614, 632, 980
ApoI RAATTY 1 cut(s) 594
AspS9I GGNCC 4 cut(s) 34, 118, 802, 868
AsuHPI GGTGA 2 cut(s) 971, 1244
AvaII GGWCC 3 cut(s) 34, 118, 868
BaeGI GKGCMC 1 cut(s) 609
BamHI GGATCC 1 cut(s) 1015
BanII GRGCYC 2 cut(s) 180, 1103
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 1103
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 3 cut(s) 619, 626, 967
BccI CCATC 3 cut(s) 341, 380, 1127
BciT130I CCWGG 4 cut(s) 38, 360, 670, 1029
BcoDI GTCTC 1 cut(s) 642
BfaI CTAG 1 cut(s) 797
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 3 cut(s) 615, 633, 981
BlsI GCNGC 3 cut(s) 616, 634, 982
Bme1390I CCNGG 4 cut(s) 38, 360, 670, 1029
Bme18I GGWCC 3 cut(s) 34, 118, 868
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgT120I GGNCC 4 cut(s) 34, 118, 802, 868
BmiI GGNNCC 5 cut(s) 177, 352, 667, 869, 1017
BmrFI CCNGG 4 cut(s) 38, 360, 670, 1029
BmrI ACTGGG 2 cut(s) 175, 340
BmsI GCATC 3 cut(s) 869, 1073, 1281
BmuI ACTGGG 2 cut(s) 175, 340
BpiI GAAGAC 1 cut(s) 266
Bpu10I CCTNAGC 3 cut(s) 240, 759, 837
BsaJI CCNNGG 3 cut(s) 252, 359, 669
BsaWI WCCGGW 1 cut(s) 644
BsaXI ACNNNNNCTCC 6 cut(s) 603, 633, 639, 669, 1024, 1054
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 1005
Bse1I ACTGG 4 cut(s) 170, 335, 354, 452
BseAI TCCGGA 1 cut(s) 644
BseBI CCWGG 4 cut(s) 38, 360, 670, 1029
BseDI CCNNGG 3 cut(s) 252, 359, 669
BseGI GGATG 5 cut(s) 66, 144, 219, 895, 1088
BseLI CCNNNNNNNGG 2 cut(s) 360, 1005
BseMII CTCAG 2 cut(s) 521, 851
BseNI ACTGG 4 cut(s) 170, 335, 354, 452
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 3 cut(s) 619, 626, 967
BsgI GTGCAG 3 cut(s) 360, 498, 954
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 3 cut(s) 804, 835, 1171
BsiHKAI GWGCWC 1 cut(s) 1103
BsiSI CCGG 2 cut(s) 645, 1281
BslFI GGGAC 3 cut(s) 278, 881, 910
BslI CCNNNNNNNGG 2 cut(s) 360, 1005
BsmAI GTCTC 1 cut(s) 642
BsmFI GGGAC 3 cut(s) 278, 881, 910
BsnI GGCC 3 cut(s) 804, 835, 1171
Bsp1286I GDGCHC 3 cut(s) 180, 609, 1103
Bsp13I TCCGGA 1 cut(s) 644
Bsp1407I TGTACA 1 cut(s) 422
Bsp143I GATC 2 cut(s) 520, 1015
BspACI CCGC 5 cut(s) 99, 153, 252, 254, 924
BspANI GGCC 3 cut(s) 804, 835, 1171
BspCNI CTCAG 2 cut(s) 522, 850
BspEI TCCGGA 1 cut(s) 644
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 5 cut(s) 177, 352, 667, 869, 1017
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 2 cut(s) 1010, 1023
BspQI GCTCTTC 2 cut(s) 303, 549
BsrGI TGTACA 1 cut(s) 422
BsrI ACTGG 4 cut(s) 170, 335, 354, 452
BssECI CCNNGG 3 cut(s) 252, 359, 669
BssMI GATC 2 cut(s) 520, 1015
Bst2UI CCWGG 4 cut(s) 38, 360, 670, 1029
Bst4CI ACNGT 3 cut(s) 228, 446, 944
Bst6I CTCTTC 4 cut(s) 303, 549, 731, 1126
BstAPI GCANNNNNTGC 1 cut(s) 977
BstAUI TGTACA 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 928
BstDEI CTNAG 5 cut(s) 240, 530, 759, 837, 1004
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 5 cut(s) 66, 144, 219, 895, 1088
BstFNI CGCG 1 cut(s) 254
BstKTI GATC 2 cut(s) 523, 1018
BstMAI GTCTC 1 cut(s) 642
BstMBI GATC 2 cut(s) 520, 1015
BstMWI GCNNNNNNNGC 2 cut(s) 932, 977
BstNI CCWGG 4 cut(s) 38, 360, 670, 1029
BstNSI RCATGY 1 cut(s) 423
BstSCI CCNGG 4 cut(s) 36, 358, 668, 1027
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 619, 626, 967
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 1015
BstYI RGATCY 1 cut(s) 1015
BsuRI GGCC 3 cut(s) 804, 835, 1171
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 5 cut(s) 66, 144, 219, 895, 1088
BtsIMutI CAGTG 3 cut(s) 451, 481, 527
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 928
Cfr13I GGNCC 4 cut(s) 34, 118, 802, 868
Cfr42I CCGCGG 1 cut(s) 255
Csp6I GTAC 3 cut(s) 423, 911, 1208
CviAII CATG 6 cut(s) 23, 323, 420, 652, 1094, 1144
CviQI GTAC 3 cut(s) 423, 911, 1208
DdeI CTNAG 5 cut(s) 240, 530, 759, 837, 1004
DpnI GATC 2 cut(s) 522, 1017
DpnII GATC 2 cut(s) 520, 1015
DraIII CACNNNGTG 1 cut(s) 815
DrdI GACNNNNNNGTC 1 cut(s) 32
DriI GACNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 32
Eam1104I CTCTTC 4 cut(s) 303, 549, 731, 1126
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 4 cut(s) 303, 549, 731, 1126
Ecl136II GAGCTC 1 cut(s) 1101
Eco24I GRGCYC 2 cut(s) 180, 1103
Eco47I GGWCC 3 cut(s) 34, 118, 868
Eco53kI GAGCTC 1 cut(s) 1101
Eco57I CTGAAG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 1101
EcoRII CCWGG 4 cut(s) 36, 358, 668, 1027
EcoT38I GRGCYC 2 cut(s) 180, 1103
FaeI CATG 6 cut(s) 26, 326, 423, 655, 1097, 1147
FaqI GGGAC 3 cut(s) 278, 881, 910
FatI CATG 6 cut(s) 22, 322, 419, 651, 1093, 1143
FauI CCCGC 2 cut(s) 106, 931
FblI GTMKAC 1 cut(s) 1024
Fnu4HI GCNGC 3 cut(s) 615, 633, 981
FokI GGATG 5 cut(s) 53, 131, 226, 902, 1095
FriOI GRGCYC 2 cut(s) 180, 1103
Fsp4HI GCNGC 3 cut(s) 615, 633, 981
FspBI CTAG 1 cut(s) 797
GluI GCNGC 3 cut(s) 615, 633, 981
HaeIII GGCC 3 cut(s) 804, 835, 1171
HapII CCGG 2 cut(s) 645, 1281
Hin1II CATG 6 cut(s) 26, 326, 423, 655, 1097, 1147
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 3 cut(s) 56, 408, 1265
HinfI GANTC 4 cut(s) 170, 674, 764, 1187
HpaII CCGG 2 cut(s) 645, 1281
HphI GGTGA 2 cut(s) 971, 1244
Hpy166II GTNNAC 5 cut(s) 136, 320, 425, 449, 1025
Hpy188I TCNGA 3 cut(s) 175, 213, 994
Hpy188III TCNNGA 6 cut(s) 52, 62, 161, 414, 645, 947
Hpy8I GTNNAC 5 cut(s) 136, 320, 425, 449, 1025
HpyAV CCTTC 7 cut(s) 8, 82, 242, 390, 476, 876, 956
HpyCH4III ACNGT 3 cut(s) 228, 446, 944
HpyCH4V TGCA 7 cut(s) 341, 436, 479, 971, 1086, 1272, 1294
HpyF10VI GCNNNNNNNGC 2 cut(s) 932, 977
HpyF3I CTNAG 5 cut(s) 240, 530, 759, 837, 1004
Hsp92II CATG 6 cut(s) 26, 326, 423, 655, 1097, 1147
Kpn2I TCCGGA 1 cut(s) 644
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 2 cut(s) 520, 1015
LguI GCTCTTC 2 cut(s) 303, 549
LmnI GCTCC 4 cut(s) 175, 611, 671, 1045
Lsp1109I GCAGC 3 cut(s) 619, 626, 967
LweI GCATC 3 cut(s) 869, 1073, 1281
MaeI CTAG 1 cut(s) 797
MaeIII GTNAC 3 cut(s) 49, 454, 842
MalI GATC 2 cut(s) 522, 1017
MboI GATC 2 cut(s) 520, 1015
MboII GAAGA 8 cut(s) 271, 317, 320, 566, 748, 783, 813, 1113
MflI RGATCY 1 cut(s) 1015
MhlI GDGCHC 3 cut(s) 180, 609, 1103
MluCI AATT 5 cut(s) 594, 686, 729, 747, 1052
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 153
MroI TCCGGA 1 cut(s) 644
MseI TTAA 1 cut(s) 732
MspA1I CMGCKG 2 cut(s) 254, 617
MspI CCGG 2 cut(s) 645, 1281
MspR9I CCNGG 4 cut(s) 38, 360, 670, 1029
MvaI CCWGG 4 cut(s) 38, 360, 670, 1029
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 932, 977
NdeII GATC 2 cut(s) 520, 1015
NlaIII CATG 6 cut(s) 26, 326, 423, 655, 1097, 1147
NlaIV GGNNCC 5 cut(s) 177, 352, 667, 869, 1017
NmuCI GTSAC 2 cut(s) 49, 842
NspI RCATGY 1 cut(s) 423
PciI ACATGT 1 cut(s) 419
PciSI GCTCTTC 2 cut(s) 303, 549
PfeI GAWTC 3 cut(s) 674, 764, 1187
PflFI GACNNNGTC 2 cut(s) 332, 653
PkrI GCNGC 3 cut(s) 616, 634, 982
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PscI ACATGT 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 1103
Psp6I CCWGG 4 cut(s) 36, 358, 668, 1027
PspGI CCWGG 4 cut(s) 36, 358, 668, 1027
PspN4I GGNNCC 5 cut(s) 177, 352, 667, 869, 1017
PspPI GGNCC 4 cut(s) 34, 118, 802, 868
PsuI RGATCY 1 cut(s) 1015
PsyI GACNNNGTC 2 cut(s) 332, 653
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 3 cut(s) 424, 912, 1209
RsaNI GTAC 3 cut(s) 423, 911, 1208
SacI GAGCTC 1 cut(s) 1103
SacII CCGCGG 1 cut(s) 255
SapI GCTCTTC 2 cut(s) 303, 549
SaqAI TTAA 1 cut(s) 732
SatI GCNGC 3 cut(s) 615, 633, 981
Sau3AI GATC 2 cut(s) 520, 1015
Sau96I GGNCC 4 cut(s) 34, 118, 802, 868
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 4 cut(s) 38, 360, 670, 1029
SduI GDGCHC 3 cut(s) 180, 609, 1103
SfaNI GCATC 3 cut(s) 869, 1073, 1281
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 3 cut(s) 34, 118, 868
Sse9I AATT 5 cut(s) 594, 686, 729, 747, 1052
SsiI CCGC 5 cut(s) 99, 153, 252, 254, 924
SspMI CTAG 1 cut(s) 797
SstI GAGCTC 1 cut(s) 1103
StyD4I CCNGG 4 cut(s) 36, 358, 668, 1027
TaaI ACNGT 3 cut(s) 228, 446, 944
TasI AATT 5 cut(s) 594, 686, 729, 747, 1052
TatI WGTACW 2 cut(s) 422, 910
TfiI GAWTC 3 cut(s) 674, 764, 1187
Tru1I TTAA 1 cut(s) 732
Tru9I TTAA 1 cut(s) 732
TscAI CASTG 3 cut(s) 451, 481, 534
TseFI GTSAC 2 cut(s) 49, 842
TseI GCWGC 3 cut(s) 614, 632, 980
Tsp45I GTSAC 2 cut(s) 49, 842
TspDTI ATGAA 4 cut(s) 355, 768, 1100, 1113
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 3 cut(s) 451, 481, 534
Tth111I GACNNNGTC 2 cut(s) 332, 653
VpaK11BI GGWCC 3 cut(s) 34, 118, 868
XapI RAATTY 1 cut(s) 594
XceI RCATGY 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 1024
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.