Rorug02G0519300

Alpha-amylase C-terminal beta-sheet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000438
Physical Location & Seq
Forward (+)
65155956 .. 65163596
7641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0519300.1

Sequence Viewer

Length: 846 bp
ATGGAAGAAAAAATGGACCCCTCAACAACCAAGTTGTTTTTCCTGGCCATACATTGCCTTGCTCTTTTGGGGCCGTTTTATTTCAACTGGTCTGCGTTTTGGCTGGCTGTGGCACTCTATTTTGTTACAGGTGTGGGTATAACTCTGTCTTTCCATAGAAACCTCGCTCACCGCAGCTTCAAGCTTCCCAGATGGCTCGAGTACTCTTTTGCCTACTGCGCCGTCCTTTCGCTCCAGGGAAGTCCAATTGAATGGGTGAGCACACACAGAATCCATCATCAGTTTACAGACACATGGGATGATCCTCACAGTCCCATTAAGGGATTTTGGTACAGTCATATTGGTTGGGTTGTTGACTATCATTCTCGGTTTGGAACTCCTGACACACTAGAATTCAAAAATGTCGGAGACTTAAAGAAGCAGTGGTACTATAGGTTTCTTCACTATACGTATCCTTGTCACTCTGTTGCTCTTGGAATTCTGCTATATGCTGGAGGAGGGCTACCATTCTTGGTTTGGGGAATGGGTGTGAGAACTGTATTTTTACTCCAGATTACTTTTTCAATAAACTCGATCTGCCACATATGGGGAAAGCAAGTATGGAATACTGGTGATTTGTCTAGAAACAACTGGTTGTTAGGGTTGCTAGCACTTGGCGAGGGTTGGCACAATAATCATCATGCCTTTGAGTACTCAGCTCGACAAGGCTTGGAATGGTGGGAGATTGATACTACTTGGTATATAATACGCTTCCTTGAAGCCATTGGTTTGGCTACAGACGTAAAACTCCCAACTGAAGCTCATAAGAAACGAAAGGCTTTATGCAAGAATCGCACTATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

281

Amino Acids

32.82

Weight (kDa)

8.52

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 29 - 250 9.8e-19 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02100
fragaria_vesca FvH4_6g46970 FvH4_6g46980 FvH4_6g46990 FvH4_6g47000
malus_domestica MD09G1064700.v1.1 MD09G1064800.v1.1 MD09G1064900.v1.1 MD09G1065000.v1.1 MD09G1065400.v1.1 MD17G1058100.v1.1 MD17G1058200.v1.1 MD17G1058300.v1.1 MD17G1058400.v1.1
prunus_persica Prupe.3G255900_v2.0.a1 Prupe.3G256000_v2.0.a1 Prupe.3G256100_v2.0.a1 Prupe.3G256200_v2.0.a1 Prupe.3G256300_v2.0.a1
pyrus_communis pycom111g05440 pycom111g05460 pycom111g05470 pycom111g05480 pycom111g05520 pycom17g05820 pycom17g05830
rosa_chinensis RchiOBHm_Chr2g0165951 RchiOBHm_Chr2g0165961 RchiOBHm_Chr2g0165981 RchiOBHm_Chr2g0165991 RchiOBHm_Chr2g0166001 RchiOBHm_Chr2g0166011 RchiOBHm_Chr2g0166021 RchiOBHm_Chr2g0166031 RchiOBHm_Chr2g0166041 RchiOBHm_Chr2g0166061
rosa_laevigata RLG00000021572 RLG00000021573 RLG00000021576 RLG00000021577 RLG00000021578 RLG00000021579 RLG00000021580
rosa_multiflora Rmu_co8157352.1_g000001 Rmu_sc0006218.1_g000013 Rmu_sc0006218.1_g000014 Rmu_sc0006218.1_g000015 Rmu_sc0006218.1_g000016 Rmu_sc0006218.1_g000017 Rmu_sc0006218.1_g000018 Rmu_sc0006218.1_g000019 Rmu_sc0006218.1_g000021 Rmu_ssc0000076.1_g000012 Rmu_ssc0000076.1_g000013
rosa_roxburghii Rroxscaffold_2G00085260 Rroxscaffold_2G00085270 Rroxscaffold_2G00085280
rosa_rugosa Rorug02G0519300 Rorug02G0519300 Rorug02G0519400 Rorug02G0519500 Rorug02G0519600 Rorug02G0519700 Rorug02G0519800 Rorug02G0519900.1
rosa_samantha Rh2AG585200 Rh2AG585300 Rh2AG585400 Rh2AG585500 Rh2AG585600 Rh2AG585700 Rh2AG585800 Rh2BG588800 Rh2BG597000 Rh2BG597100 Rh2BG597200 Rh2BG597300 Rh2BG597400 Rh2BG597500 Rh2CG567800 Rh2CG567900 Rh2CG568000 Rh2CG568100 Rh2DG607100 Rh2DG607200 Rh2DG607400 Rh2DG607500 Rh2DG607600
rosa_wichuraiana Rw2G047970 Rw2G048790 Rw2G048800 Rw2G048810 Rw2G048820 Rw2G048830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 172
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 2 cut(s) 392, 477
AcuI CTGAAG 1 cut(s) 816
AfaI GTAC 4 cut(s) 203, 332, 428, 692
AfiI CCNNNNNNNGG 2 cut(s) 320, 586
AgsI TTSAA 6 cut(s) 85, 181, 251, 397, 564, 758
AjnI CCWGG 2 cut(s) 42, 234
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 4 cut(s) 177, 184, 698, 800
AluI AGCT 4 cut(s) 177, 184, 698, 800
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 45, 71
ApeKI GCWGC 1 cut(s) 174
ApoI RAATTY 2 cut(s) 392, 477
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 16, 71
AsuHPI GGTGA 3 cut(s) 161, 268, 623
AsuNHI GCTAGC 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 1 cut(s) 16
BalI TGGCCA 1 cut(s) 47
BarI GAAGNNNNNNTAC 2 cut(s) 410, 442
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 186
BccI CCATC 2 cut(s) 186, 282
BceAI ACGGC 2 cut(s) 58, 206
BciT130I CCWGG 2 cut(s) 44, 236
BciVI GTATCC 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 3 cut(s) 389, 621, 647
BfmI CTRYAG 2 cut(s) 430, 774
BfuI GTATCC 1 cut(s) 462
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
BmcAI AGTACT 2 cut(s) 203, 692
Bme1390I CCNGG 2 cut(s) 44, 236
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 16, 71
BmiI GGNNCC 2 cut(s) 18, 72
BmrFI CCNGG 2 cut(s) 44, 236
BmtI GCTAGC 1 cut(s) 650
BpmI CTGGAG 3 cut(s) 218, 513, 533
BsaAI YACGTR 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 586
Bse1I ACTGG 3 cut(s) 92, 613, 635
Bse3DI GCAATG 1 cut(s) 52
BseBI CCWGG 2 cut(s) 44, 236
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 320, 586
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 708
BseNI ACTGG 3 cut(s) 92, 613, 635
BseRI GAGGAG 1 cut(s) 510
BseXI GCAGC 1 cut(s) 186
BshFI GGCC 2 cut(s) 47, 73
BsiHKAI GWGCWC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 197
BslFI GGGAC 1 cut(s) 297
BslI CCNNNNNNNGG 2 cut(s) 320, 586
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 297
BsnI GGCC 2 cut(s) 47, 73
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 2 cut(s) 301, 573
BspACI CCGC 1 cut(s) 172
BspANI GGCC 2 cut(s) 47, 73
BspCNI CTCAG 1 cut(s) 707
BspLI GGNNCC 2 cut(s) 18, 72
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 52
BsrI ACTGG 3 cut(s) 92, 613, 635
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 2 cut(s) 301, 573
Bst2UI CCWGG 2 cut(s) 44, 236
Bst4CI ACNGT 3 cut(s) 311, 335, 538
BstBAI YACGTR 1 cut(s) 450
BstC8I GCNNGC 2 cut(s) 105, 648
BstDEI CTNAG 1 cut(s) 694
BstF5I GGATG 1 cut(s) 304
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 2 cut(s) 304, 576
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 2 cut(s) 301, 573
BstMWI GCNNNNNNNGC 2 cut(s) 218, 831
BstNI CCWGG 2 cut(s) 44, 236
BstSCI CCNGG 2 cut(s) 42, 234
BstSFI CTRYAG 2 cut(s) 430, 774
BstSNI TACGTA 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 186
BstXI CCANNNNNNTGG 2 cut(s) 252, 769
BsuI GTATCC 1 cut(s) 462
BsuRI GGCC 2 cut(s) 47, 73
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 2 cut(s) 105, 648
CfoI GCGC 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 16, 71
Csp6I GTAC 4 cut(s) 202, 331, 427, 691
CviAII CATG 2 cut(s) 294, 680
CviQI GTAC 4 cut(s) 202, 331, 427, 691
DdeI CTNAG 1 cut(s) 694
DpnI GATC 2 cut(s) 303, 575
DpnII GATC 2 cut(s) 301, 573
EaeI YGGCCR 1 cut(s) 45
Eco105I TACGTA 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 816
Eco88I CYCGRG 1 cut(s) 197
EcoRI GAATTC 2 cut(s) 392, 477
EcoRII CCWGG 2 cut(s) 42, 234
FaeI CATG 2 cut(s) 297, 683
FaqI GGGAC 1 cut(s) 297
FatI CATG 2 cut(s) 293, 679
FauNDI CATATG 1 cut(s) 584
Fnu4HI GCNGC 1 cut(s) 175
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 175
FspBI CTAG 3 cut(s) 389, 621, 647
GlaI GCGC 1 cut(s) 220
GluI GCNGC 1 cut(s) 175
GsuI CTGGAG 3 cut(s) 218, 513, 533
HaeIII GGCC 2 cut(s) 47, 73
HhaI GCGC 1 cut(s) 221
Hin1II CATG 2 cut(s) 297, 683
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 2 cut(s) 270, 829
HphI GGTGA 3 cut(s) 161, 268, 623
Hpy166II GTNNAC 2 cut(s) 285, 355
Hpy188I TCNGA 1 cut(s) 407
Hpy188III TCNNGA 3 cut(s) 380, 550, 621
Hpy8I GTNNAC 2 cut(s) 285, 355
HpyCH4III ACNGT 3 cut(s) 311, 335, 538
HpyCH4IV ACGT 2 cut(s) 449, 780
HpyCH4V TGCA 1 cut(s) 825
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 831
HpyF3I CTNAG 1 cut(s) 694
HpySE526I ACGT 2 cut(s) 449, 780
Hsp92II CATG 2 cut(s) 297, 683
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 2 cut(s) 301, 573
LmnI GCTCC 1 cut(s) 237
Lsp1109I GCAGC 1 cut(s) 186
MaeI CTAG 3 cut(s) 389, 621, 647
MaeII ACGT 2 cut(s) 449, 780
MaeIII GTNAC 2 cut(s) 124, 458
MalI GATC 2 cut(s) 303, 575
MboI GATC 2 cut(s) 301, 573
MboII GAAGA 2 cut(s) 17, 431
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 3 cut(s) 246, 392, 477
MluNI TGGCCA 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 6 cut(s) 31, 173, 315, 488, 491, 652
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
MseI TTAA 2 cut(s) 318, 413
Msp20I TGGCCA 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 44, 236
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 2 cut(s) 44, 236
MwoI GCNNNNNNNGC 2 cut(s) 218, 831
NdeI CATATG 1 cut(s) 584
NdeII GATC 2 cut(s) 301, 573
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 2 cut(s) 297, 683
NlaIV GGNNCC 2 cut(s) 18, 72
NmuCI GTSAC 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 197
PfeI GAWTC 2 cut(s) 270, 829
PkrI GCNGC 1 cut(s) 176
Ppu21I YACGTR 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 42, 234
PspGI CCWGG 2 cut(s) 42, 234
PspN4I GGNNCC 2 cut(s) 18, 72
PspPI GGNCC 2 cut(s) 16, 71
PspXI VCTCGAGB 1 cut(s) 197
RsaI GTAC 4 cut(s) 203, 332, 428, 692
RsaNI GTAC 4 cut(s) 202, 331, 427, 691
SaqAI TTAA 2 cut(s) 318, 413
SatI GCNGC 1 cut(s) 175
Sau3AI GATC 2 cut(s) 301, 573
Sau96I GGNCC 2 cut(s) 16, 71
ScaI AGTACT 2 cut(s) 203, 692
ScrFI CCNGG 2 cut(s) 44, 236
SduI GDGCHC 1 cut(s) 263
SetI ASST 9 cut(s) 133, 165, 179, 186, 437, 452, 700, 783, 802
SfcI CTRYAG 2 cut(s) 430, 774
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SnaBI TACGTA 1 cut(s) 450
Sse9I AATT 3 cut(s) 246, 392, 477
SsiI CCGC 1 cut(s) 172
SspMI CTAG 3 cut(s) 389, 621, 647
StyD4I CCNGG 2 cut(s) 42, 234
TaaI ACNGT 3 cut(s) 311, 335, 538
TaiI ACGT 2 cut(s) 452, 783
TaqI TCGA 3 cut(s) 198, 572, 700
TasI AATT 3 cut(s) 246, 392, 477
TatI WGTACW 2 cut(s) 201, 690
TfiI GAWTC 2 cut(s) 270, 829
Tru1I TTAA 2 cut(s) 318, 413
Tru9I TTAA 2 cut(s) 318, 413
TscAI CASTG 1 cut(s) 428
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 1 cut(s) 174
Tsp45I GTSAC 1 cut(s) 458
TspRI CASTG 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 392, 477
XbaI TCTAGA 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XhoI CTCGAG 1 cut(s) 197
XspI CTAG 3 cut(s) 389, 621, 647
ZrmI AGTACT 2 cut(s) 203, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.