Rw2G048800

UDP-glycosyltransferase 83A1-like

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
Physical Location & Seq
Forward (+)
76344792 .. 76346432
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G048800.1

Sequence Viewer

Length: 1332 bp
ATGAGCAAGCCACATATTTTAGCTATTCCTTACCCAGCACAAGGCCATGTAATTCCCTTGATGGAGTTATCACAGTGCTTAATAAATCATGGCTTCAAAGTCACATTCGTGAATACGGATCATACTCACGAGCGAGTCATGAATGCTGTAGCTGATGAAACTCATAGAAGAGATGATCAGCTTCATCTGGTTTCAATTCCAGATGGGTTAGAACCATGGGAGGACAGGAATGAGCTGGGTCAGTTATCTGAAGCAATATACCGAGTGATGCCTGGGAAGTTGGAGCAGCTCATAGAGAAGATCAATGGAGAGGAAGGTGACAAAATAACTTGTGTCATTGCTGATTTCATTATGGGATGGGCTCTGGAAGTGGCCGAGAAAAAGAATATCAAGAGTGTTTCCTTTTTCCCTGCGGCAGCTGCAGTTCTTGCGTTGAATTTTTGTGTTCCACAGTTAATATGTGTAGGAATCATTAATAATGATGGAACTGTATCAAAAGACCAAGTGATTCACTTGGCACCAAACATGCCCATCATGAAGACCGAAGACATGCCGTGGTTATGCTTTGATGGCTTAGCCACTCAGAAAATTATGTTTCAAGTCGGACAAAGAAACAACAGAGCTGTGGAACTGGCAGGCTGGGTTATTTGCAACTCAACATTTGACCTCGAACCAGCAGCATTTGCCTTGGCACCCGAGATTTTACCAATAGGCCCTCTTTTGGCAAGCAGCCGGCTTGGAAAAACAGCAGGAAACTTCTGGCCAGCGGATTCCACTTGCTTGGATTGGCTCGATCAACAACCACCCTGCTTAGTCATCTATGTTGCATTTGGAAGCTTCACAGTTTTTGATCAAACTCAGTTCCAAGAATTGGCTTTAGGTCTTGAACTATCCAATAGGCCATTCCTTACTCCCTATCCAGAAGGATACCAAGAACGAGTAGGCTCTAAGGGAATGATGGTGGGATGGGCACCCCAACAGAAGGTTCTATCCCATCCTTCAATTGCTTGCTTCCTAAGTCACTGTGGTTGGAACTCTACCATGGAAGGTGTAAGCAATGGGCTTCCTTTCTTGTGTTGGCCATATTTTGCTGACCAGCTCTGTAACGAGGGCTACATTTGCGATGTTTGGGAGGTGGGATTGAGGTTTGATAAGAATGAGAGTGGAATCATCCCGAAAGGAGAAATCAAGAACAAGGTGGAACAACTTCTTGGAGACGAAAATTATAGAGCAAGGGCTTCTAAACTCAAGGAAATGGCCTTGACTAATGTCAAAGAAGGAGGCCAATCTAACAAGATCTTCAAGAATTTAATTGAATGGATAAAATCGTAG
Functional Annotation

Protein Analysis

443

Amino Acids

49.43

Weight (kDa)

5.18

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 272 - 417 7.6e-20 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Orthologous Genes (Group: OG0000438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02100
fragaria_vesca FvH4_6g46970 FvH4_6g46980 FvH4_6g46990 FvH4_6g47000
malus_domestica MD09G1064700.v1.1 MD09G1064800.v1.1 MD09G1064900.v1.1 MD09G1065000.v1.1 MD09G1065400.v1.1 MD17G1058100.v1.1 MD17G1058200.v1.1 MD17G1058300.v1.1 MD17G1058400.v1.1
prunus_persica Prupe.3G255900_v2.0.a1 Prupe.3G256000_v2.0.a1 Prupe.3G256100_v2.0.a1 Prupe.3G256200_v2.0.a1 Prupe.3G256300_v2.0.a1
pyrus_communis pycom111g05440 pycom111g05460 pycom111g05470 pycom111g05480 pycom111g05520 pycom17g05820 pycom17g05830
rosa_chinensis RchiOBHm_Chr2g0165951 RchiOBHm_Chr2g0165961 RchiOBHm_Chr2g0165981 RchiOBHm_Chr2g0165991 RchiOBHm_Chr2g0166001 RchiOBHm_Chr2g0166011 RchiOBHm_Chr2g0166021 RchiOBHm_Chr2g0166031 RchiOBHm_Chr2g0166041 RchiOBHm_Chr2g0166061
rosa_laevigata RLG00000021572 RLG00000021573 RLG00000021576 RLG00000021577 RLG00000021578 RLG00000021579 RLG00000021580
rosa_multiflora Rmu_co8157352.1_g000001 Rmu_sc0006218.1_g000013 Rmu_sc0006218.1_g000014 Rmu_sc0006218.1_g000015 Rmu_sc0006218.1_g000016 Rmu_sc0006218.1_g000017 Rmu_sc0006218.1_g000018 Rmu_sc0006218.1_g000019 Rmu_sc0006218.1_g000021 Rmu_ssc0000076.1_g000012 Rmu_ssc0000076.1_g000013
rosa_roxburghii Rroxscaffold_2G00085260 Rroxscaffold_2G00085270 Rroxscaffold_2G00085280
rosa_rugosa Rorug02G0519300 Rorug02G0519300 Rorug02G0519400 Rorug02G0519500 Rorug02G0519600 Rorug02G0519700 Rorug02G0519800 Rorug02G0519900.1
rosa_samantha Rh2AG585200 Rh2AG585300 Rh2AG585400 Rh2AG585500 Rh2AG585600 Rh2AG585700 Rh2AG585800 Rh2BG588800 Rh2BG597000 Rh2BG597100 Rh2BG597200 Rh2BG597300 Rh2BG597400 Rh2BG597500 Rh2CG567800 Rh2CG567900 Rh2CG568000 Rh2CG568100 Rh2DG607100 Rh2DG607200 Rh2DG607400 Rh2DG607500 Rh2DG607600
rosa_wichuraiana Rw2G047970 Rw2G048790 Rw2G048800 Rw2G048810 Rw2G048820 Rw2G048830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 517, 691, 970
AccB7I CCANNNNNTGG 1 cut(s) 871
AciI CCGC 2 cut(s) 413, 767
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 3 cut(s) 372, 761, 1079
AcsI RAATTY 2 cut(s) 436, 1306
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 4 cut(s) 41, 721, 871, 982
AgsI TTSAA 8 cut(s) 97, 195, 436, 599, 887, 1002, 1303, 1316
AjnI CCWGG 1 cut(s) 271
AjuI GAANNNNNNNTTGG 6 cut(s) 887, 919, 969, 1001, 1194, 1226
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 9 cut(s) 23, 152, 181, 235, 289, 419, 623, 837, 1099
AluI AGCT 9 cut(s) 23, 152, 181, 235, 289, 419, 623, 837, 1099
Alw26I GTCTC 1 cut(s) 1209
AlwI GGATC 1 cut(s) 126
Ama87I CYCGRG 1 cut(s) 695
AoxI GGCC 8 cut(s) 43, 372, 712, 761, 899, 1079, 1257, 1282
ApeKI GCWGC 5 cut(s) 286, 416, 419, 677, 729
ApoI RAATTY 2 cut(s) 436, 1306
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 1206
AspS9I GGNCC 1 cut(s) 713
AsuHPI GGTGA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 973
BalI TGGCCA 2 cut(s) 763, 1081
BanI GGYRCC 3 cut(s) 517, 691, 970
BanII GRGCYC 1 cut(s) 364
BauI CACGAG 1 cut(s) 128
BbsI GAAGAC 2 cut(s) 545, 552
BbvI GCAGC 5 cut(s) 298, 406, 428, 689, 741
BccI CCATC 9 cut(s) 55, 197, 351, 476, 539, 563, 952, 960, 1002
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 1 cut(s) 273
BciVI GTATCC 1 cut(s) 920
BclI TGATCA 2 cut(s) 175, 850
BcoDI GTCTC 1 cut(s) 1209
BfmI CTRYAG 2 cut(s) 147, 420
BfuI GTATCC 1 cut(s) 920
BglII AGATCT 1 cut(s) 1296
BisI GCNGC 6 cut(s) 287, 414, 417, 420, 678, 730
BlpI GCTNAGC 1 cut(s) 574
BlsI GCNGC 6 cut(s) 288, 415, 418, 421, 679, 731
Bme1390I CCNGG 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 695
BmgT120I GGNCC 1 cut(s) 713
BmiI GGNNCC 3 cut(s) 519, 693, 972
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 1268
BpiI GAAGAC 2 cut(s) 545, 552
Bpu1102I GCTNAGC 1 cut(s) 574
BpuEI CTTGAG 1 cut(s) 1232
BsaJI CCNNGG 5 cut(s) 215, 272, 554, 687, 1041
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 721, 871, 982
Bse118I RCCGGY 1 cut(s) 732
Bse1I ACTGG 1 cut(s) 636
Bse3DI GCAATG 2 cut(s) 336, 1063
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 5 cut(s) 215, 272, 554, 687, 1041
BseGI GGATG 4 cut(s) 362, 971, 994, 1170
BseLI CCNNNNNNNGG 4 cut(s) 41, 721, 871, 982
BseMI GCAATG 2 cut(s) 336, 1063
BseMII CTCAG 2 cut(s) 596, 872
BseNI ACTGG 1 cut(s) 636
BseSI GKGCMC 1 cut(s) 973
BseXI GCAGC 5 cut(s) 298, 406, 428, 689, 741
BseYI CCCAGC 3 cut(s) 34, 235, 639
BshFI GGCC 8 cut(s) 45, 374, 714, 763, 901, 1081, 1259, 1284
BshNI GGYRCC 3 cut(s) 517, 691, 970
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 1 cut(s) 733
BslI CCNNNNNNNGG 4 cut(s) 41, 721, 871, 982
BsmAI GTCTC 1 cut(s) 1209
BsmBI CGTCTC 1 cut(s) 1209
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 8 cut(s) 45, 374, 714, 763, 901, 1081, 1259, 1284
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 2 cut(s) 364, 973
Bsp143I GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
Bsp1720I GCTNAGC 1 cut(s) 574
Bsp19I CCATGG 2 cut(s) 215, 1041
BspACI CCGC 2 cut(s) 413, 767
BspANI GGCC 8 cut(s) 45, 374, 714, 763, 901, 1081, 1259, 1284
BspCNI CTCAG 2 cut(s) 595, 871
BspHI TCATGA 2 cut(s) 138, 534
BspLI GGNNCC 3 cut(s) 519, 693, 972
BspMAI CTGCAG 1 cut(s) 424
BspPI GGATC 1 cut(s) 126
BspT107I GGYRCC 3 cut(s) 517, 691, 970
BsrDI GCAATG 2 cut(s) 336, 1063
BsrFI RCCGGY 1 cut(s) 732
BsrI ACTGG 1 cut(s) 636
BssAI RCCGGY 1 cut(s) 732
BssECI CCNNGG 5 cut(s) 215, 272, 554, 687, 1041
BssMI GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 3 cut(s) 215, 687, 1041
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 5 cut(s) 75, 453, 490, 844, 1025
Bst6I CTCTTC 1 cut(s) 163
BstAPI GCANNNNNTGC 2 cut(s) 428, 683
BstC8I GCNNGC 6 cut(s) 8, 637, 727, 734, 765, 1009
BstDEI CTNAG 6 cut(s) 574, 582, 811, 858, 948, 1016
BstDSI CCRYGG 3 cut(s) 215, 554, 1041
BstF5I GGATG 4 cut(s) 362, 971, 994, 1170
BstKTI GATC 6 cut(s) 121, 178, 303, 796, 853, 1299
BstMAI GTCTC 1 cut(s) 1209
BstMBI GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
BstMWI GCNNNNNNNGC 5 cut(s) 419, 428, 570, 683, 1119
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 2 cut(s) 529, 553
BstPAI GACNNNNGTC 1 cut(s) 1268
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 2 cut(s) 147, 420
BstSLI GKGCMC 1 cut(s) 973
BstV1I GCAGC 5 cut(s) 298, 406, 428, 689, 741
BstV2I GAAGAC 2 cut(s) 545, 552
BstX2I RGATCY 1 cut(s) 1296
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 1 cut(s) 1296
BsuI GTATCC 1 cut(s) 920
BsuRI GGCC 8 cut(s) 45, 374, 714, 763, 901, 1081, 1259, 1284
BtgI CCRYGG 3 cut(s) 215, 554, 1041
BtgZI GCGATG 1 cut(s) 1137
BtsCI GGATG 4 cut(s) 362, 971, 994, 1170
BtsIMutI CAGTG 2 cut(s) 80, 1021
Cac8I GCNNGC 6 cut(s) 8, 637, 727, 734, 765, 1009
CciI TCATGA 2 cut(s) 138, 534
Cfr10I RCCGGY 1 cut(s) 732
Cfr13I GGNCC 1 cut(s) 713
CviAII CATG 8 cut(s) 47, 89, 139, 216, 526, 535, 550, 1042
DdeI CTNAG 6 cut(s) 574, 582, 811, 858, 948, 1016
DpnI GATC 6 cut(s) 120, 177, 302, 795, 852, 1298
DpnII GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
EaeI YGGCCR 3 cut(s) 372, 761, 1079
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 215, 687, 1041
Eco24I GRGCYC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 271
EcoT14I CCWWGG 3 cut(s) 215, 687, 1041
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 3 cut(s) 215, 687, 1041
Esp3I CGTCTC 1 cut(s) 1209
FaeI CATG 8 cut(s) 50, 92, 142, 219, 529, 538, 553, 1045
FatI CATG 8 cut(s) 46, 88, 138, 215, 525, 534, 549, 1041
FbaI TGATCA 2 cut(s) 175, 850
Fnu4HI GCNGC 6 cut(s) 287, 414, 417, 420, 678, 730
FokI GGATG 4 cut(s) 369, 978, 981, 1157
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 6 cut(s) 287, 414, 417, 420, 678, 730
GluI GCNGC 6 cut(s) 287, 414, 417, 420, 678, 730
GsaI CCCAGC 3 cut(s) 38, 239, 643
HaeIII GGCC 8 cut(s) 45, 374, 714, 763, 901, 1081, 1259, 1284
HapII CCGG 1 cut(s) 733
Hin1II CATG 8 cut(s) 50, 92, 142, 219, 529, 538, 553, 1045
HindIII AAGCTT 1 cut(s) 835
HinfI GANTC 5 cut(s) 135, 468, 508, 770, 1167
HpaII CCGG 1 cut(s) 733
HphI GGTGA 1 cut(s) 329
Hpy188I TCNGA 3 cut(s) 250, 585, 605
HpyAV CCTTC 6 cut(s) 308, 917, 976, 1008, 1040, 1271
HpyCH4III ACNGT 5 cut(s) 75, 453, 490, 844, 1025
HpyCH4V TGCA 3 cut(s) 422, 651, 827
HpyF10VI GCNNNNNNNGC 5 cut(s) 419, 428, 570, 683, 1119
HpyF3I CTNAG 6 cut(s) 574, 582, 811, 858, 948, 1016
Hsp92II CATG 8 cut(s) 50, 92, 142, 219, 529, 538, 553, 1045
KroI GCCGGC 1 cut(s) 732
KroNI GCCGGC 1 cut(s) 734
Ksp22I TGATCA 2 cut(s) 175, 850
Kzo9I GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 5 cut(s) 298, 406, 428, 689, 741
LweI GCATC 1 cut(s) 258
MaeIII GTNAC 4 cut(s) 100, 317, 1019, 1103
MalI GATC 6 cut(s) 120, 177, 302, 795, 852, 1298
MboI GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
MboII GAAGA 5 cut(s) 180, 310, 550, 557, 1291
MfeI CAATTG 1 cut(s) 1002
MflI RGATCY 1 cut(s) 1296
MhlI GDGCHC 2 cut(s) 364, 973
MlsI TGGCCA 2 cut(s) 763, 1081
MluCI AATT 9 cut(s) 51, 195, 436, 588, 869, 1002, 1222, 1306, 1311
MluNI TGGCCA 2 cut(s) 763, 1081
MlyI GAGTC 1 cut(s) 144
MmeI TCCRAC 3 cut(s) 261, 583, 1010
MnlI CCTC 8 cut(s) 214, 304, 677, 726, 1102, 1126, 1137, 1274
Mox20I TGGCCA 2 cut(s) 763, 1081
MroNI GCCGGC 1 cut(s) 732
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 2 cut(s) 763, 1081
MseI TTAA 4 cut(s) 80, 455, 474, 1310
MslI CAYNNNNRTG 1 cut(s) 107
Msp20I TGGCCA 2 cut(s) 763, 1081
MspA1I CMGCKG 2 cut(s) 419, 767
MspI CCGG 1 cut(s) 733
MspR9I CCNGG 1 cut(s) 273
MunI CAATTG 1 cut(s) 1002
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 5 cut(s) 419, 428, 570, 683, 1119
NaeI GCCGGC 1 cut(s) 734
NcoI CCATGG 2 cut(s) 215, 1041
NdeII GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
NgoMIV GCCGGC 1 cut(s) 732
NlaIII CATG 8 cut(s) 50, 92, 142, 219, 529, 538, 553, 1045
NlaIV GGNNCC 3 cut(s) 519, 693, 972
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 3 cut(s) 100, 317, 1019
NspI RCATGY 2 cut(s) 529, 553
OliI CACNNNNGTG 1 cut(s) 107
PagI TCATGA 2 cut(s) 138, 534
PctI GAATGC 1 cut(s) 148
PdiI GCCGGC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 4 cut(s) 468, 508, 770, 1167
PflMI CCANNNNNTGG 1 cut(s) 871
PkrI GCNGC 6 cut(s) 288, 415, 418, 421, 679, 731
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PshAI GACNNNNGTC 1 cut(s) 1268
PshBI ATTAAT 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 271
PspFI CCCAGC 3 cut(s) 34, 235, 639
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 3 cut(s) 519, 693, 972
PspPI GGNCC 1 cut(s) 713
PstI CTGCAG 1 cut(s) 424
PsuI RGATCY 1 cut(s) 1296
PvuII CAGCTG 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 4 cut(s) 80, 455, 474, 1310
SatI GCNGC 6 cut(s) 287, 414, 417, 420, 678, 730
Sau3AI GATC 6 cut(s) 118, 175, 300, 793, 850, 1296
Sau96I GGNCC 1 cut(s) 713
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 1 cut(s) 273
SduI GDGCHC 2 cut(s) 364, 973
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 2 cut(s) 147, 420
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 1247
SmoI CTYRAG 1 cut(s) 1247
Sse9I AATT 9 cut(s) 51, 195, 436, 588, 869, 1002, 1222, 1306, 1311
SsiI CCGC 2 cut(s) 413, 767
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 3 cut(s) 215, 687, 1041
TaaI ACNGT 5 cut(s) 75, 453, 490, 844, 1025
TaqI TCGA 2 cut(s) 669, 792
TaqII GACCGA 1 cut(s) 557
TasI AATT 9 cut(s) 51, 195, 436, 588, 869, 1002, 1222, 1306, 1311
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 4 cut(s) 468, 508, 770, 1167
Tru1I TTAA 4 cut(s) 80, 455, 474, 1310
Tru9I TTAA 4 cut(s) 80, 455, 474, 1310
TscAI CASTG 2 cut(s) 80, 1028
TseFI GTSAC 3 cut(s) 100, 317, 1019
TseI GCWGC 5 cut(s) 286, 416, 419, 677, 729
Tsp45I GTSAC 3 cut(s) 100, 317, 1019
TspDTI ATGAA 5 cut(s) 155, 171, 173, 337, 551
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 2 cut(s) 80, 1028
Van91I CCANNNNNTGG 1 cut(s) 871
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 2 cut(s) 436, 1306
XceI RCATGY 2 cut(s) 529, 553
XmnI GAANNNNTTC 1 cut(s) 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.