Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003013

Physical Location & Seq

Genomic Coordinates Reverse (-)

4503693 .. 4508791

Gene Structure 5099 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1066100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGGACGCCGACGGAAATACCGAAGAGCCGTCGCGGCTGGTCCTCGTCCGCTTCAAAACGGACCTCGGCCACCCCTACAAAGTTACAGAATCCTCCATTTCCATCCCCGCCAATCTCACCCGCTTCGGCCTCTCCACCGTTGTCAACAACCTCATCAAATCAGGAAACCCCGATTGGGAATCGGAGCCTTTCGATTTTCTTATCAAGAATGAGCTGGTTCGGATGTCGCTCGAAAAGTTCCTTCTTGCCAAGAACATTTCTGCGGAGAGGATATTGGAAATCGAATATATACGGGCTGTGGTCCCACGCAAAGAAGAAGAACCTTCTTTACATAATGACTGGGTCAGTGCAGTTGATGGTTCTAGTCCCAGGTTCTTTTTGACAGGCTGCTATGATGGTTTGGGAAGGGTATGGAAAGCTGCCGGAGCATGTACACATGTTCTGGAAGGACACAGTGAACCTGTTACTTCAGTTAGTATAATCAATCCAAAAGGTGGAGAAAGTGTTACCGTAGCTACTGCTTCGAAAGATCGGACACTGAGACTGTGGAAGTTCAATGCAGAAGAGACCCGAAAAAATCCTTTGAAGATAAGAGCATTCAAAATTTTACGTGGACATACAGCAGCTGTTCAAAGTGTTGCTGCTCAGACCTCTGGAAACATGGTCTGTTCAGGCTCTTGGGATTCTACTATCAATTTATGGCAGACAAATGAGCCTGATTCAGAAGGTGATACTGTGTCAACGAAGAAGAGAAAAAAGACTGGTCAAGAAAAGGAATCGCAGTGGGAGGGGGAGGCTGTTTCTTCTCTAATAGGCCATACACAATGCGTATCTACTGTGAAGTGGCCAGAGCCTAAAACAATTTATTCTGCATCGTGGGACCATTCCATTAAAGAGTGGAATGTCGAGAGAGGCACAGCCACGTCGACAATATATCATGATAAAGTACTCAACTGCATTGATATTGGAGGTGAAGGTTCTGCTTTGATTGCTGCTGGTGGTTCTGACCCCATTGTTAGGGTATGGGATCCTCGTAAACCAGGAACTTCTGCTTCTATCAAAACTTTCTCATCTCACACTTCCTGGGTTACGGCATGCAAGTGGCATGAGAAGTCTCCATTTCATTTAGTCTCTGCATCTTATGACGGAAAAGTTATGCTGTGGGATACGAGAACTGCTTGGCCCTTGTTTGTCTTTGATTCACACAAGGACAAGGCGCTATGCGCTGACTGGTGGAAAGGTGATACTGTGCTTAGTGGTGGTGCAGATTCCAAGCTTTGCATGACTTCCGGGGTTTCTGTGCTCCATAAGCAGAAGAGTGATTTGAAGATGGAAGGACTTTTGCATCGCTTACAACTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000460 GO:0000470 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030054 GO:0030684 GO:0030686 GO:0030687 GO:0031461 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NLE WD40

Protein Analysis

455

Amino Acids

50.23

Weight (kDa)

7.22

Isoelectric Point (pI)

35.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NLE	PF08154	14 - 81	1.7e-18	NLE (NUC135) domain
Beta-prop_WDR5	PF25175	111 - 238	3e-17	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	112 - 238	7.1e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	112 - 190	3.5e-06	THOC3 beta-propeller domain
WD40_Prp19	PF24814	118 - 238	4.7e-12	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	120 - 240	8.1e-11	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	125 - 235	1.5e-10	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	126 - 238	3e-06	WDR3 second beta-propeller domain
WD40	PF00400	142 - 183	2.4e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	152 - 342	3.3e-13	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	170 - 250	2.1e-10	THOC3 beta-propeller domain
WD40	PF00400	201 - 234	6.5e-07	WD domain, G-beta repeat
WDR55	PF24796	268 - 424	2e-08	WDR55
Beta-prop_WDR5	PF25175	270 - 424	1.2e-20	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	272 - 425	2.2e-13	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	272 - 392	1.8e-07	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	283 - 419	5.1e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	300 - 425	1.4e-12	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	313 - 427	4.2e-13	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	313 - 427	6.1e-15	THOC3 beta-propeller domain
WD40_Prp19	PF24814	317 - 405	6.1e-09	Prp19 WD40 domain
WD40	PF00400	352 - 389	8.6e-08	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15550 AT5G15550 AT5G15550
fragaria_vesca	FvH4_6g46921 FvH4_6g46921 FvH4_6g46921 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930 FvH4_6g46930
malus_domestica	MD09G1066000.v1.1 MD09G1066100.v1.1 MD17G1058700.v1.1 MD17G1058800.v1.1
prunus_persica	Prupe.3G255400_v2.0.a1 Prupe.3G255500_v2.0.a1
pyrus_communis	pycom111g05550 pycom17g05870
rosa_chinensis	RchiOBHm_Chr2g0165901 RchiOBHm_Chr2g0165911
rosa_laevigata	RLG00000021569
rosa_multiflora	Rmu_co8422767.1_g000001 Rmu_sc0006218.1_g000010
rosa_roxburghii	Rroxscaffold_2G00085310 Rroxscaffold_2G00085320
rosa_rugosa	Rorug02G0519200 Rorug02G0519300
rosa_samantha	Rh2AG584700 Rh2AG584800 Rh2BG596600 Rh2CG567400 Rh2DG606700 Rh2DG606800
rosa_wichuraiana	Rw2G048750 Rw2G048760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	494
AccI	GTMKAC	1 cut(s)	926
AccII	CGCG	1 cut(s)	34
AciI	CCGC	5 cut(s)	34, 49, 108, 121, 263
AclWI	GGATC	2 cut(s)	1022, 1035
AcoI	YGGCCR	2 cut(s)	67, 845
AcsI	RAATTY	1 cut(s)	603
AcuI	CTGAAG	1 cut(s)	453
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	2 cut(s)	433, 948
AfiI	CCNNNNNNNGG	3 cut(s)	175, 494, 1017
AflIII	ACRYGT	1 cut(s)	436
AgsI	TTSAA	6 cut(s)	55, 556, 586, 601, 632, 1327
AjiI	CACGTC	1 cut(s)	924
AjnI	CCWGG	3 cut(s)	368, 1039, 1082
AjuI	GAANNNNNNNTTGG	2 cut(s)	157, 189
AluBI	AGCT	5 cut(s)	214, 419, 515, 626, 1276
AluI	AGCT	5 cut(s)	214, 419, 515, 626, 1276
Alw21I	GWGCWC	1 cut(s)	1305
Alw26I	GTCTC	4 cut(s)	535, 560, 1119, 1135
AlwI	GGATC	2 cut(s)	1022, 1035
AlwNI	CAGNNNCTG	1 cut(s)	626
AoxI	GGCC	5 cut(s)	67, 127, 814, 845, 1181
ApeKI	GCWGC	5 cut(s)	387, 419, 623, 641, 992
ApoI	RAATTY	1 cut(s)	603
AspLEI	GCGC	2 cut(s)	1219, 1226
AspS9I	GGNCC	5 cut(s)	40, 61, 301, 880, 1182
AsuC2I	CCSGG	1 cut(s)	1291
AsuHPI	GGTGA	4 cut(s)	109, 740, 983, 1253
AsuII	TTCGAA	1 cut(s)	524
AvaII	GGWCC	4 cut(s)	40, 61, 301, 880
BalI	TGGCCA	1 cut(s)	847
BamHI	GGATCC	1 cut(s)	1027
Bbv12I	GWGCWC	1 cut(s)	1305
BbvI	GCAGC	5 cut(s)	374, 406, 628, 635, 979
BccI	CCATC	4 cut(s)	110, 350, 389, 1324
BceAI	ACGGC	2 cut(s)	13, 1107
BciT130I	CCWGG	3 cut(s)	370, 1041, 1084
BciVI	GTATCC	1 cut(s)	1159
BcnI	CCSGG	1 cut(s)	1291
BcoDI	GTCTC	4 cut(s)	535, 560, 1119, 1135
BfaI	CTAG	1 cut(s)	363
BfoI	RGCGCY	1 cut(s)	1220
BfuI	GTATCC	1 cut(s)	1159
BglI	GCCNNNNNGGC	1 cut(s)	34
BisI	GCNGC	6 cut(s)	35, 388, 420, 624, 642, 993
BlsI	GCNGC	6 cut(s)	36, 389, 421, 625, 643, 994
BmcAI	AGTACT	1 cut(s)	948
Bme1390I	CCNGG	4 cut(s)	370, 1041, 1084, 1291
Bme18I	GGWCC	4 cut(s)	40, 61, 301, 880
BmgBI	CACGTC	1 cut(s)	924
BmgT120I	GGNCC	5 cut(s)	40, 61, 301, 880, 1182
BmiI	GGNNCC	4 cut(s)	186, 303, 881, 1029
BmrFI	CCNGG	4 cut(s)	370, 1041, 1084, 1291
BmrI	ACTGGG	1 cut(s)	349
BmsI	GCATC	3 cut(s)	881, 1145, 1354
BmuI	ACTGGG	1 cut(s)	349
Bpu14I	TTCGAA	1 cut(s)	524
BpuMI	CCSGG	1 cut(s)	1291
BsaAI	YACGTR	1 cut(s)	611
BsaHI	GRCGYC	1 cut(s)	6
BsaI	GGTCTC	1 cut(s)	560
BsaJI	CCNNGG	4 cut(s)	64, 368, 1083, 1290
BsaXI	ACNNNNNCTCC	2 cut(s)	489, 519
Bsc4I	CCNNNNNNNGG	3 cut(s)	175, 494, 1017
Bse1I	ACTGG	3 cut(s)	344, 766, 1235
BseBI	CCWGG	3 cut(s)	370, 1041, 1084
BseDI	CCNNGG	4 cut(s)	64, 368, 1083, 1290
BseGI	GGATG	2 cut(s)	102, 228
BseLI	CCNNNNNNNGG	3 cut(s)	175, 494, 1017
BseMII	CTCAG	2 cut(s)	530, 659
BseNI	ACTGG	3 cut(s)	344, 766, 1235
BseXI	GCAGC	5 cut(s)	374, 406, 628, 635, 979
BsgI	GTGCAG	2 cut(s)	369, 1284
Bsh1236I	CGCG	1 cut(s)	34
BshFI	GGCC	5 cut(s)	69, 129, 816, 847, 1183
BsiHKAI	GWGCWC	1 cut(s)	1305
BsiSI	CCGG	2 cut(s)	423, 1290
BslFI	GGGAC	3 cut(s)	287, 351, 893
BslI	CCNNNNNNNGG	3 cut(s)	175, 494, 1017
BsmAI	GTCTC	4 cut(s)	535, 560, 1119, 1135
BsmFI	GGGAC	3 cut(s)	287, 351, 893
BsmI	GAATGC	1 cut(s)	596
BsnI	GGCC	5 cut(s)	69, 129, 816, 847, 1183
Bso31I	GGTCTC	1 cut(s)	560
Bsp119I	TTCGAA	1 cut(s)	524
Bsp1286I	GDGCHC	1 cut(s)	1305
Bsp1407I	TGTACA	1 cut(s)	431
Bsp143I	GATC	2 cut(s)	529, 1027
BspACI	CCGC	5 cut(s)	34, 49, 108, 121, 263
BspANI	GGCC	5 cut(s)	69, 129, 816, 847, 1183
BspCNI	CTCAG	2 cut(s)	531, 658
BspFNI	CGCG	1 cut(s)	34
BspHI	TCATGA	1 cut(s)	937
BspLI	GGNNCC	4 cut(s)	186, 303, 881, 1029
BspPI	GGATC	2 cut(s)	1022, 1035
BspQI	GCTCTTC	1 cut(s)	18
BspT104I	TTCGAA	1 cut(s)	524
BspTNI	GGTCTC	1 cut(s)	560
BsrGI	TGTACA	1 cut(s)	431
BsrI	ACTGG	3 cut(s)	344, 766, 1235
BssECI	CCNNGG	4 cut(s)	64, 368, 1083, 1290
BssMI	GATC	2 cut(s)	529, 1027
BssNI	GRCGYC	1 cut(s)	6
Bst2UI	CCWGG	3 cut(s)	370, 1041, 1084
Bst4CI	ACNGT	7 cut(s)	139, 455, 511, 546, 736, 838, 1249
Bst6I	CTCTTC	4 cut(s)	18, 558, 743, 1310
BstACI	GRCGYC	1 cut(s)	6
BstAUI	TGTACA	1 cut(s)	431
BstBAI	YACGTR	1 cut(s)	611
BstBI	TTCGAA	1 cut(s)	524
BstC8I	GCNNGC	1 cut(s)	1096
BstDEI	CTNAG	3 cut(s)	539, 645, 1253
BstF5I	GGATG	2 cut(s)	102, 228
BstFNI	CGCG	1 cut(s)	34
BstH2I	RGCGCY	1 cut(s)	1220
BstHHI	GCGC	2 cut(s)	1219, 1226
BstKTI	GATC	2 cut(s)	532, 1030
BstMAI	GTCTC	4 cut(s)	535, 560, 1119, 1135
BstMBI	GATC	2 cut(s)	529, 1027
BstMWI	GCNNNNNNNGC	5 cut(s)	34, 425, 989, 1223, 1309
BstNI	CCWGG	3 cut(s)	370, 1041, 1084
BstNSI	RCATGY	3 cut(s)	432, 440, 1098
BstSCI	CCNGG	4 cut(s)	368, 1039, 1082, 1289
BstUI	CGCG	1 cut(s)	34
BstV1I	GCAGC	5 cut(s)	374, 406, 628, 635, 979
BstX2I	RGATCY	1 cut(s)	1027
BstYI	RGATCY	1 cut(s)	1027
BsuI	GTATCC	1 cut(s)	1159
BsuRI	GGCC	5 cut(s)	69, 129, 816, 847, 1183
BtgZI	GCGATG	1 cut(s)	1331
BtrI	CACGTC	1 cut(s)	924
BtsCI	GGATG	2 cut(s)	102, 228
BtsI	GCAGTG	1 cut(s)	788
BtsIMutI	CAGTG	4 cut(s)	352, 460, 536, 788
Cac8I	GCNNGC	1 cut(s)	1096
CaiI	CAGNNNCTG	1 cut(s)	626
CciI	TCATGA	1 cut(s)	937
CfoI	GCGC	2 cut(s)	1219, 1226
Cfr13I	GGNCC	5 cut(s)	40, 61, 301, 880, 1182
CseI	GACGC	1 cut(s)	14
Csp6I	GTAC	2 cut(s)	432, 947
CviAII	CATG	7 cut(s)	429, 437, 661, 938, 1095, 1106, 1282
CviQI	GTAC	2 cut(s)	432, 947
DdeI	CTNAG	3 cut(s)	539, 645, 1253
DpnI	GATC	2 cut(s)	531, 1029
DpnII	GATC	2 cut(s)	529, 1027
EaeI	YGGCCR	2 cut(s)	67, 845
Eam1104I	CTCTTC	4 cut(s)	18, 558, 743, 1310
EarI	CTCTTC	4 cut(s)	18, 558, 743, 1310
Eco31I	GGTCTC	1 cut(s)	560
Eco47I	GGWCC	4 cut(s)	40, 61, 301, 880
Eco57I	CTGAAG	1 cut(s)	453
EcoRII	CCWGG	3 cut(s)	368, 1039, 1082
FaeI	CATG	7 cut(s)	432, 440, 664, 941, 1098, 1109, 1285
FaqI	GGGAC	3 cut(s)	287, 351, 893
FatI	CATG	7 cut(s)	428, 436, 660, 937, 1094, 1105, 1281
FauI	CCCGC	2 cut(s)	115, 128
FblI	GTMKAC	1 cut(s)	926
Fnu4HI	GCNGC	6 cut(s)	35, 388, 420, 624, 642, 993
FokI	GGATG	2 cut(s)	89, 235
Fsp4HI	GCNGC	6 cut(s)	35, 388, 420, 624, 642, 993
FspBI	CTAG	1 cut(s)	363
GlaI	GCGC	2 cut(s)	1218, 1225
GluI	GCNGC	6 cut(s)	35, 388, 420, 624, 642, 993
HaeII	RGCGCY	1 cut(s)	1220
HaeIII	GGCC	5 cut(s)	69, 129, 816, 847, 1183
HapII	CCGG	2 cut(s)	423, 1290
HgaI	GACGC	1 cut(s)	14
HhaI	GCGC	2 cut(s)	1219, 1226
Hin1I	GRCGYC	1 cut(s)	6
Hin1II	CATG	7 cut(s)	432, 440, 664, 941, 1098, 1109, 1285
Hin6I	GCGC	2 cut(s)	1217, 1224
HinP1I	GCGC	2 cut(s)	1217, 1224
HincII	GTYRAC	3 cut(s)	145, 741, 927
HindII	GTYRAC	3 cut(s)	145, 741, 927
HindIII	AAGCTT	1 cut(s)	1274
HinfI	GANTC	7 cut(s)	89, 179, 683, 719, 776, 1199, 1268
HpaII	CCGG	2 cut(s)	423, 1290
HphI	GGTGA	4 cut(s)	109, 740, 983, 1253
Hpy166II	GTNNAC	7 cut(s)	145, 434, 458, 614, 741, 927, 1037
Hpy188I	TCNGA	6 cut(s)	184, 222, 534, 648, 724, 1006
Hpy188III	TCNNGA	7 cut(s)	162, 205, 443, 654, 767, 907, 938
Hpy8I	GTNNAC	7 cut(s)	145, 434, 458, 614, 741, 927, 1037
Hpy99I	CGWCG	3 cut(s)	14, 34, 928
HpyAV	CCTTC	7 cut(s)	251, 333, 399, 440, 719, 968, 1328
HpyCH4III	ACNGT	7 cut(s)	139, 455, 511, 546, 736, 838, 1249
HpyCH4IV	ACGT	2 cut(s)	610, 923
HpyCH4V	TGCA	9 cut(s)	350, 560, 872, 957, 1098, 1136, 1265, 1281, 1345
HpyF10VI	GCNNNNNNNGC	5 cut(s)	34, 425, 989, 1223, 1309
HpyF3I	CTNAG	3 cut(s)	539, 645, 1253
HpySE526I	ACGT	2 cut(s)	610, 923
Hsp92I	GRCGYC	1 cut(s)	6
Hsp92II	CATG	7 cut(s)	432, 440, 664, 941, 1098, 1109, 1285
HspAI	GCGC	2 cut(s)	1217, 1224
Kzo9I	GATC	2 cut(s)	529, 1027
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	3 cut(s)	184, 425, 1308
Lsp1109I	GCAGC	5 cut(s)	374, 406, 628, 635, 979
LweI	GCATC	3 cut(s)	881, 1145, 1354
MaeI	CTAG	1 cut(s)	363
MaeII	ACGT	2 cut(s)	610, 923
MaeIII	GTNAC	4 cut(s)	82, 463, 505, 1087
MalI	GATC	2 cut(s)	531, 1029
MboI	GATC	2 cut(s)	529, 1027
MflI	RGATCY	1 cut(s)	1027
MhlI	GDGCHC	1 cut(s)	1305
MlsI	TGGCCA	1 cut(s)	847
MluCI	AATT	3 cut(s)	603, 694, 861
MluNI	TGGCCA	1 cut(s)	847
Mox20I	TGGCCA	1 cut(s)	847
MscI	TGGCCA	1 cut(s)	847
MseI	TTAA	1 cut(s)	891
MslI	CAYNNNNRTG	1 cut(s)	1099
Msp20I	TGGCCA	1 cut(s)	847
MspA1I	CMGCKG	1 cut(s)	626
MspI	CCGG	2 cut(s)	423, 1290
MspR9I	CCNGG	4 cut(s)	370, 1041, 1084, 1291
Mva1269I	GAATGC	1 cut(s)	596
MvaI	CCWGG	3 cut(s)	370, 1041, 1084
MvnI	CGCG	1 cut(s)	34
MwoI	GCNNNNNNNGC	5 cut(s)	34, 425, 989, 1223, 1309
NciI	CCSGG	1 cut(s)	1291
NdeII	GATC	2 cut(s)	529, 1027
NlaIII	CATG	7 cut(s)	432, 440, 664, 941, 1098, 1109, 1285
NlaIV	GGNNCC	4 cut(s)	186, 303, 881, 1029
NmeAIII	GCCGAG	1 cut(s)	45
NspI	RCATGY	3 cut(s)	432, 440, 1098
NspV	TTCGAA	1 cut(s)	524
PaeI	GCATGC	1 cut(s)	1098
PagI	TCATGA	1 cut(s)	937
PciI	ACATGT	1 cut(s)	436
PciSI	GCTCTTC	1 cut(s)	18
PcsI	WCGNNNNNNNCGW	1 cut(s)	18
PctI	GAATGC	1 cut(s)	596
PfeI	GAWTC	7 cut(s)	89, 179, 683, 719, 776, 1199, 1268
PflFI	GACNNNGTC	1 cut(s)	341
PflMI	CCANNNNNTGG	1 cut(s)	494
PkrI	GCNGC	6 cut(s)	36, 389, 421, 625, 643, 994
Ppu21I	YACGTR	1 cut(s)	611
PscI	ACATGT	1 cut(s)	436
Psp6I	CCWGG	3 cut(s)	368, 1039, 1082
PspGI	CCWGG	3 cut(s)	368, 1039, 1082
PspN4I	GGNNCC	4 cut(s)	186, 303, 881, 1029
PspPI	GGNCC	5 cut(s)	40, 61, 301, 880, 1182
PsrI	GAACNNNNNNTAC	2 cut(s)	312, 344
PstNI	CAGNNNCTG	1 cut(s)	626
PsuI	RGATCY	1 cut(s)	1027
PsyI	GACNNNGTC	1 cut(s)	341
PvuII	CAGCTG	1 cut(s)	626
RsaI	GTAC	2 cut(s)	433, 948
RsaNI	GTAC	2 cut(s)	432, 947
RseI	CAYNNNNRTG	1 cut(s)	1099
SalI	GTCGAC	1 cut(s)	925
SapI	GCTCTTC	1 cut(s)	18
SaqAI	TTAA	1 cut(s)	891
SatI	GCNGC	6 cut(s)	35, 388, 420, 624, 642, 993
Sau3AI	GATC	2 cut(s)	529, 1027
Sau96I	GGNCC	5 cut(s)	40, 61, 301, 880, 1182
ScaI	AGTACT	1 cut(s)	948
ScrFI	CCNGG	4 cut(s)	370, 1041, 1084, 1291
SduI	GDGCHC	1 cut(s)	1305
SfaNI	GCATC	3 cut(s)	881, 1145, 1354
SfuI	TTCGAA	1 cut(s)	524
SinI	GGWCC	4 cut(s)	40, 61, 301, 880
SmiMI	CAYNNNNRTG	1 cut(s)	1099
SphI	GCATGC	1 cut(s)	1098
Sse9I	AATT	3 cut(s)	603, 694, 861
SsiI	CCGC	5 cut(s)	34, 49, 108, 121, 263
SspMI	CTAG	1 cut(s)	363
StyD4I	CCNGG	4 cut(s)	368, 1039, 1082, 1289
TaaI	ACNGT	7 cut(s)	139, 455, 511, 546, 736, 838, 1249
TaiI	ACGT	2 cut(s)	613, 926
TaqI	TCGA	6 cut(s)	192, 231, 282, 524, 906, 926
TasI	AATT	3 cut(s)	603, 694, 861
TatI	WGTACW	2 cut(s)	431, 946
TauI	GCSGC	1 cut(s)	37
TfiI	GAWTC	7 cut(s)	89, 179, 683, 719, 776, 1199, 1268
Tru1I	TTAA	1 cut(s)	891
Tru9I	TTAA	1 cut(s)	891
TscAI	CASTG	4 cut(s)	352, 460, 543, 788
TseI	GCWGC	5 cut(s)	387, 419, 623, 641, 992
TspDTI	ATGAA	1 cut(s)	1112
TspGWI	ACGGA	3 cut(s)	27, 74, 1161
TspRI	CASTG	4 cut(s)	352, 460, 543, 788
Tth111I	GACNNNGTC	1 cut(s)	341
Van91I	CCANNNNNTGG	1 cut(s)	494
VpaK11BI	GGWCC	4 cut(s)	40, 61, 301, 880
XapI	RAATTY	1 cut(s)	603
XceI	RCATGY	3 cut(s)	432, 440, 1098
XmiI	GTMKAC	1 cut(s)	926
XspI	CTAG	1 cut(s)	363
ZrmI	AGTACT	1 cut(s)	948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.