MD09G1166500.v1.1

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008700
Physical Location & Seq
Forward (+)
13646629 .. 13652279
5651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1166500.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGTCGTTCGCAGCAACACCAGCGACAACGATGACGACATGCCGCTTCGTTACGCTTCGAATTCAGTGCACTGATTCCAAGCCCAGAACTGGTTTCGGAAGCAAAACCAACAGCAAGAACAAGAACAGGAACAAGAACAACCTCAGCCAGGAATCAACTACCAAACGGTCTGGTGGCGTTCCTACTCAGGCACCCGGGTTAAGTTCTCGGTTCGATGGAAAGGTTAAGAGAAACCCTGCTGAGCTTGAGTTTGAGGAACGTCTAGCAGCAGTCAGAAGTTCAGCAATTGAGCAGAAGAAGGTAGTGGAGAAAAAGGAGTTTGGGCCAATTGACTATGATGCACCTGTTGAGTTGGAGAAAACGGATAAGAAAAAAATTGGACTCGGTGCACAGATTGGAGTAGGTGTAGCTGTAGTGGTCTTTGGTTTGGTTTTTGCTCTTGGAGACTTTCTTCCTTCTGGAAGTTTGAGTCCTACTGAGGATACTGCAGCAACTGCCAATAAACTGTCCGAAGAAGAGAAAGCGTCACTTCAGACTAGGCTGAAAGAATATGAAGCAACGCTTAGTAACTCCCCAAAAGATCCAACTGCTCTTGAGGGAGCTGCAGTAACCTTAGCAGAATTAGGAGAATATACACAGGCTTCAACTCTGCTTCAAGACTTGATTAAGGAGAAACCAAGTGATCCTGAAGTTTTTCGTTTGCTTGGAGAAGTTAAATATGAACTCAAGGATTTTGAAGAAAGTGCTGCTGCATTTAAGGTTTCTTCATCGATGTCTAAAGATCTCAAATTTGAAGTTCTGCGTGGCCTTACGAATGCATTACTTGCTGCTAAAAAACCAGATGAGGCTGTTCAATTTCTTCTTGCCTCTCATGAACGTATGAATGTGGACAAGCCAGACACAAAGGCTGGAAGTAGTGCGACTGAAACAAATTCGCAGGTGGACCCTATTCAAGTCGAATTACTTCTTGGAAAAGCCTATTCAGATTGGGGCCATATCAGTGATGCTGTTTCTGTCTATGACCGGCTCATCTCTAGTCATCCAAATGACTTCCGCGGTTACTTGGCCAAGGGAATTATTTTGAGAGAAAATGGTAAAGCTGGAGAAGCGGAGAGGATGTTTATACAGGCACGGTTCTTTGCACCGGATAAAGCCAAAGCATTTGTAGACCGATACTCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_6 TPR_8

Protein Analysis

395

Amino Acids

43.03

Weight (kDa)

7.64

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_19 PF14559 184 - 236 3.3e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 928
Acc36I ACCTGC 1 cut(s) 928
AccB1I GGYRCC 1 cut(s) 190
AccI GTMKAC 1 cut(s) 1167
AccII CGCG 1 cut(s) 1056
AciI CCGC 4 cut(s) 43, 1054, 1056, 1109
AclWI GGATC 2 cut(s) 575, 677
AcoI YGGCCR 1 cut(s) 1065
AcsI RAATTY 3 cut(s) 60, 788, 931
AcuI CTGAAG 2 cut(s) 515, 708
AfiI CCNNNNNNNGG 2 cut(s) 89, 148
AgsI TTSAA 6 cut(s) 645, 656, 737, 794, 854, 953
AjnI CCWGG 1 cut(s) 147
AjuI GAANNNNNNNTTGG 2 cut(s) 951, 983
AluBI AGCT 4 cut(s) 244, 410, 602, 1100
AluI AGCT 4 cut(s) 244, 410, 602, 1100
Alw21I GWGCWC 2 cut(s) 71, 391
Alw26I GTCTC 1 cut(s) 438
Alw44I GTGCAC 2 cut(s) 67, 387
AlwI GGATC 2 cut(s) 575, 677
AlwNI CAGNNNCTG 1 cut(s) 494
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 4 cut(s) 323, 805, 991, 1065
ApaLI GTGCAC 2 cut(s) 67, 387
ApeKI GCWGC 7 cut(s) 11, 266, 488, 602, 746, 749, 827
ApoI RAATTY 3 cut(s) 60, 788, 931
Asp700I GAANNNNTTC 2 cut(s) 693, 963
AspS9I GGNCC 3 cut(s) 323, 943, 991
AsuC2I CCSGG 2 cut(s) 195, 196
AsuII TTCGAA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 943
BaeGI GKGCMC 2 cut(s) 71, 391
BalI TGGCCA 1 cut(s) 1067
BanI GGYRCC 1 cut(s) 190
Bbv12I GWGCWC 2 cut(s) 71, 391
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 7 cut(s) 23, 278, 500, 589, 733, 736, 814
BccI CCATC 1 cut(s) 209
BcgI CGANNNNNNTGC 2 cut(s) 48, 82
BciT130I CCWGG 1 cut(s) 149
BciVI GTATCC 1 cut(s) 475
BcnI CCSGG 2 cut(s) 195, 196
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 3 cut(s) 263, 537, 1035
BfmI CTRYAG 3 cut(s) 411, 486, 603
BfuAI ACCTGC 1 cut(s) 928
BfuI GTATCC 1 cut(s) 475
BglII AGATCT 1 cut(s) 781
BisI GCNGC 8 cut(s) 12, 43, 267, 489, 603, 747, 750, 828
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 8 cut(s) 13, 44, 268, 490, 604, 748, 751, 829
Bme1390I CCNGG 3 cut(s) 149, 195, 196
Bme18I GGWCC 1 cut(s) 943
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 3 cut(s) 323, 943, 991
BmiI GGNNCC 3 cut(s) 192, 945, 992
BmrFI CCNGG 3 cut(s) 149, 195, 196
BmsI GCATC 2 cut(s) 328, 994
BpmI CTGGAG 1 cut(s) 1122
Bpu10I CCTNAGC 2 cut(s) 143, 613
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 58
BpuEI CTTGAG 4 cut(s) 266, 614, 710, 1162
BpuMI CCSGG 2 cut(s) 195, 196
Bsa29I ATCGAT 1 cut(s) 770
BsaJI CCNNGG 3 cut(s) 194, 1054, 1068
BsaWI WCCGGW 1 cut(s) 1144
BsaXI ACNNNNNCTCC 6 cut(s) 390, 420, 591, 621, 1103, 1133
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 148
Bse118I RCCGGY 1 cut(s) 1023
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 149
BseCI ATCGAT 1 cut(s) 770
BseDI CCNNGG 3 cut(s) 194, 1054, 1068
BseGI GGATG 2 cut(s) 1039, 1122
BseLI CCNNNNNNNGG 2 cut(s) 89, 148
BseMII CTCAG 4 cut(s) 157, 200, 231, 468
BseNI ACTGG 1 cut(s) 94
BseSI GKGCMC 2 cut(s) 71, 391
BseXI GCAGC 7 cut(s) 23, 278, 500, 589, 733, 736, 814
Bsh1236I CGCG 1 cut(s) 1056
BshFI GGCC 4 cut(s) 325, 807, 993, 1067
BshNI GGYRCC 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 770
BsiHKAI GWGCWC 2 cut(s) 71, 391
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 3 cut(s) 195, 1024, 1145
BslI CCNNNNNNNGG 2 cut(s) 89, 148
BsmAI GTCTC 1 cut(s) 438
BsmI GAATGC 1 cut(s) 820
BsnI GGCC 4 cut(s) 325, 807, 993, 1067
BsoBI CYCGRG 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 58
Bsp1286I GDGCHC 2 cut(s) 71, 391
Bsp143I GATC 3 cut(s) 580, 682, 781
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 4 cut(s) 43, 1054, 1056, 1109
BspANI GGCC 4 cut(s) 325, 807, 993, 1067
BspCNI CTCAG 4 cut(s) 156, 199, 232, 469
BspDI ATCGAT 1 cut(s) 770
BspFNI CGCG 1 cut(s) 1056
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 3 cut(s) 192, 945, 992
BspMAI CTGCAG 2 cut(s) 490, 607
BspMI ACCTGC 1 cut(s) 928
BspPI GGATC 2 cut(s) 575, 677
BspT104I TTCGAA 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 1023
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 1023
BssECI CCNNGG 3 cut(s) 194, 1054, 1068
BssMI GATC 3 cut(s) 580, 682, 781
BssT1I CCWWGG 1 cut(s) 1068
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 3 cut(s) 168, 507, 1134
Bst6I CTCTTC 1 cut(s) 510
BstAPI GCANNNNNTGC 2 cut(s) 494, 824
BstBI TTCGAA 1 cut(s) 58
BstDEI CTNAG 6 cut(s) 143, 186, 240, 477, 563, 613
BstDSI CCRYGG 1 cut(s) 1054
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 2 cut(s) 1039, 1122
BstFNI CGCG 1 cut(s) 1056
BstKTI GATC 3 cut(s) 583, 685, 784
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 3 cut(s) 580, 682, 781
BstMWI GCNNNNNNNGC 4 cut(s) 20, 494, 824, 1106
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 3 cut(s) 147, 193, 194
BstSFI CTRYAG 3 cut(s) 411, 486, 603
BstSLI GKGCMC 2 cut(s) 71, 391
BstUI CGCG 1 cut(s) 1056
BstV1I GCAGC 7 cut(s) 23, 278, 500, 589, 733, 736, 814
BstX2I RGATCY 2 cut(s) 580, 781
BstYI RGATCY 2 cut(s) 580, 781
Bsu15I ATCGAT 1 cut(s) 770
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 4 cut(s) 325, 807, 993, 1067
BsuTUI ATCGAT 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 1054
BtsCI GGATG 2 cut(s) 1039, 1122
BtsIMutI CAGTG 3 cut(s) 69, 71, 1006
BveI ACCTGC 1 cut(s) 928
CaiI CAGNNNCTG 1 cut(s) 494
CciI TCATGA 1 cut(s) 871
Cfr10I RCCGGY 1 cut(s) 1023
Cfr13I GGNCC 3 cut(s) 323, 943, 991
Cfr42I CCGCGG 1 cut(s) 1057
Cfr9I CCCGGG 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 770
CseI GACGC 1 cut(s) 513
CviAII CATG 2 cut(s) 39, 872
DdeI CTNAG 6 cut(s) 143, 186, 240, 477, 563, 613
DpnI GATC 3 cut(s) 582, 684, 783
DpnII GATC 3 cut(s) 580, 682, 781
EaeI YGGCCR 1 cut(s) 1065
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 1068
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 2 cut(s) 515, 708
Eco88I CYCGRG 1 cut(s) 194
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 1068
EcoT22I ATGCAT 1 cut(s) 820
ErhI CCWWGG 1 cut(s) 1068
FaeI CATG 2 cut(s) 42, 875
FalI AAGNNNNNCTT 4 cut(s) 513, 545, 546, 578
FatI CATG 2 cut(s) 38, 871
FblI GTMKAC 1 cut(s) 1167
Fnu4HI GCNGC 8 cut(s) 12, 43, 267, 489, 603, 747, 750, 828
FokI GGATG 2 cut(s) 1026, 1129
Fsp4HI GCNGC 8 cut(s) 12, 43, 267, 489, 603, 747, 750, 828
FspBI CTAG 3 cut(s) 263, 537, 1035
GluI GCNGC 8 cut(s) 12, 43, 267, 489, 603, 747, 750, 828
GsuI CTGGAG 1 cut(s) 1122
HaeIII GGCC 4 cut(s) 325, 807, 993, 1067
HapII CCGG 3 cut(s) 195, 1024, 1145
HgaI GACGC 1 cut(s) 513
Hin1II CATG 2 cut(s) 42, 875
HinfI GANTC 4 cut(s) 74, 152, 381, 469
HpaII CCGG 3 cut(s) 195, 1024, 1145
Hpy166II GTNNAC 5 cut(s) 69, 389, 889, 943, 1168
Hpy188I TCNGA 5 cut(s) 98, 275, 511, 534, 985
Hpy188III TCNNGA 6 cut(s) 459, 593, 656, 686, 872, 1179
Hpy8I GTNNAC 5 cut(s) 69, 389, 889, 943, 1168
HpyAV CCTTC 2 cut(s) 292, 465
HpyCH4III ACNGT 3 cut(s) 168, 507, 1134
HpyCH4IV ACGT 2 cut(s) 259, 877
HpyCH4V TGCA 8 cut(s) 69, 341, 389, 488, 605, 752, 818, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 494, 824, 1106
HpyF3I CTNAG 6 cut(s) 143, 186, 240, 477, 563, 613
HpySE526I ACGT 2 cut(s) 259, 877
Hsp92II CATG 2 cut(s) 42, 875
KspI CCGCGG 1 cut(s) 1057
Kzo9I GATC 3 cut(s) 580, 682, 781
LmnI GCTCC 1 cut(s) 599
Lsp1109I GCAGC 7 cut(s) 23, 278, 500, 589, 733, 736, 814
LweI GCATC 2 cut(s) 328, 994
MaeI CTAG 3 cut(s) 263, 537, 1035
MaeII ACGT 2 cut(s) 259, 877
MaeIII GTNAC 5 cut(s) 49, 525, 566, 607, 1058
MalI GATC 3 cut(s) 582, 684, 783
MboI GATC 3 cut(s) 580, 682, 781
MboII GAAGA 7 cut(s) 307, 443, 524, 527, 749, 756, 851
MfeI CAATTG 2 cut(s) 285, 327
MflI RGATCY 2 cut(s) 580, 781
MhlI GDGCHC 2 cut(s) 71, 391
MlsI TGGCCA 1 cut(s) 1067
MluNI TGGCCA 1 cut(s) 1067
MlyI GAGTC 2 cut(s) 375, 478
MmeI TCCRAC 2 cut(s) 333, 608
MnlI CCTC 7 cut(s) 152, 247, 472, 589, 838, 877, 1107
Mox20I TGGCCA 1 cut(s) 1067
Mph1103I ATGCAT 1 cut(s) 820
MroXI GAANNNNTTC 2 cut(s) 693, 963
MscI TGGCCA 1 cut(s) 1067
MseI TTAA 5 cut(s) 200, 225, 666, 714, 756
MslI CAYNNNNRTG 2 cut(s) 999, 1044
Msp20I TGGCCA 1 cut(s) 1067
MspA1I CMGCKG 1 cut(s) 1056
MspI CCGG 3 cut(s) 195, 1024, 1145
MspR9I CCNGG 3 cut(s) 149, 195, 196
MunI CAATTG 2 cut(s) 285, 327
Mva1269I GAATGC 1 cut(s) 820
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 1056
MwoI GCNNNNNNNGC 4 cut(s) 20, 494, 824, 1106
NciI CCSGG 2 cut(s) 195, 196
NdeII GATC 3 cut(s) 580, 682, 781
NlaIII CATG 2 cut(s) 42, 875
NlaIV GGNNCC 3 cut(s) 192, 945, 992
NmuCI GTSAC 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 42
NspV TTCGAA 1 cut(s) 58
PagI TCATGA 1 cut(s) 871
PaqCI CACCTGC 1 cut(s) 928
PctI GAATGC 1 cut(s) 820
PdmI GAANNNNTTC 2 cut(s) 693, 963
PfeI GAWTC 2 cut(s) 74, 152
PkrI GCNGC 8 cut(s) 13, 44, 268, 490, 604, 748, 751, 829
PleI GAGTC 2 cut(s) 375, 477
PpsI GAGTC 2 cut(s) 375, 477
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 3 cut(s) 192, 945, 992
PspPI GGNCC 3 cut(s) 323, 943, 991
PstI CTGCAG 2 cut(s) 490, 607
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 2 cut(s) 580, 781
RseI CAYNNNNRTG 2 cut(s) 999, 1044
SacII CCGCGG 1 cut(s) 1057
SaqAI TTAA 5 cut(s) 200, 225, 666, 714, 756
SatI GCNGC 8 cut(s) 12, 43, 267, 489, 603, 747, 750, 828
Sau3AI GATC 3 cut(s) 580, 682, 781
Sau96I GGNCC 3 cut(s) 323, 943, 991
SchI GAGTC 2 cut(s) 375, 478
ScrFI CCNGG 3 cut(s) 149, 195, 196
SduI GDGCHC 2 cut(s) 71, 391
SfaNI GCATC 2 cut(s) 328, 994
SfcI CTRYAG 3 cut(s) 411, 486, 603
Sfr303I CCGCGG 1 cut(s) 1057
SfuI TTCGAA 1 cut(s) 58
SgrBI CCGCGG 1 cut(s) 1057
SinI GGWCC 1 cut(s) 943
SmaI CCCGGG 1 cut(s) 196
SmiMI CAYNNNNRTG 2 cut(s) 999, 1044
SmlI CTYRAG 4 cut(s) 245, 593, 725, 1177
SmoI CTYRAG 4 cut(s) 245, 593, 725, 1177
SsiI CCGC 4 cut(s) 43, 1054, 1056, 1109
SspMI CTAG 3 cut(s) 263, 537, 1035
StyD4I CCNGG 3 cut(s) 147, 193, 194
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 3 cut(s) 168, 507, 1134
TaiI ACGT 2 cut(s) 262, 880
TaqI TCGA 4 cut(s) 58, 213, 770, 957
TaqII GACCGA 1 cut(s) 1185
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 2 cut(s) 74, 152
Tru1I TTAA 5 cut(s) 200, 225, 666, 714, 756
Tru9I TTAA 5 cut(s) 200, 225, 666, 714, 756
TscAI CASTG 3 cut(s) 71, 76, 1006
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 7 cut(s) 11, 266, 488, 602, 746, 749, 827
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 5 cut(s) 567, 735, 756, 888, 896
TspGWI ACGGA 1 cut(s) 377
TspMI CCCGGG 1 cut(s) 194
TspRI CASTG 3 cut(s) 71, 76, 1006
VneI GTGCAC 2 cut(s) 67, 387
VpaK11BI GGWCC 1 cut(s) 943
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 3 cut(s) 60, 788, 931
XceI RCATGY 1 cut(s) 42
XmaI CCCGGG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 1167
XmnI GAANNNNTTC 2 cut(s) 693, 963
XspI CTAG 3 cut(s) 263, 537, 1035
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.