MD09G1178400.v1.1

Protein FLUORESCENT IN BLUE LIGHT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003675
Physical Location & Seq
Forward (+)
15259134 .. 15261229
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1178400.v1.1.491

Sequence Viewer

Length: 705 bp
ATGTCAGGTATGACGGGAGATGTAAAGTTGAGATTCCCAGCAATGGGACTTCTTCTCGGCAATTCCTTAATGTTGACAACACCATTGGAAGCTTTGGCGGAAGCATGCGAGGCTGACGACTCGGGTTTCAGCATGCCTATACTGCTATTTGTAGCCCTTGTAGGGGCCACTGTTGGTGGCTTGGTTGCTCGGCAAAGGAAAGGAGAATTACAGCGAGTGAATGAACAGTTGCGTCAAATCAATCAATCTCTAAGGAGGCAAGCTAAGATCGAGTCATATGCTCCTAGTTTGAGTTATTCACCGATTGGTGCAAAAATACTACCGGAGAGTGAGGTGATAGTTGAGCCAAGGAAGCACGAGTTGATTTCTCGACTGAAAGCTGGGAAGAATTTTTTGAGAAATCAAGAAACGGAGAAAGCACTAGATGAGTTTAAGACAGCTCTTGAGCTTGCCCAAAGTATGAAGGACCCAATTGAGGAAAAGAAGGCTGCAAGAGGTTTAGGAGCCTCACTGCAAAGATTGGGCAAGTACCGAGACGCTATTAAGTGCCATTCTATGGTGTTGGACATCTCCAAGCGAGAAGGAGAAGACTCAGGAAACACAGAAGCTTATGGAGCGATTGCTGATTGTTATACTGAGCTTGGAGATCTCGAGCGTGCAGGGAAGTTCTATGACAATTACATTGCGAGGTTGGAGTCCGACTGA

Protein Analysis

235

Amino Acids

25.77

Weight (kDa)

5.89

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 164 - 229 3.9e-09 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 556
AciI CCGC 1 cut(s) 98
AcsI RAATTY 1 cut(s) 388
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 6 cut(s) 43, 44, 162, 163, 475, 556
AluBI AGCT 7 cut(s) 92, 263, 380, 440, 448, 608, 640
AluI AGCT 7 cut(s) 92, 263, 380, 440, 448, 608, 640
Alw26I GTCTC 1 cut(s) 528
Ama87I CYCGRG 2 cut(s) 121, 650
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 388
AspS9I GGNCC 2 cut(s) 165, 466
AsuHPI GGTGA 2 cut(s) 291, 346
AvaI CYCGRG 2 cut(s) 121, 650
AvaII GGWCC 1 cut(s) 466
BauI CACGAG 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 594
BbvI GCAGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BcoDI GTCTC 1 cut(s) 528
BfaI CTAG 2 cut(s) 285, 422
BglII AGATCT 1 cut(s) 646
BisI GCNGC 1 cut(s) 489
BlsI GCNGC 1 cut(s) 490
Bme18I GGWCC 1 cut(s) 466
BmeT110I CYCGRG 2 cut(s) 121, 650
BmgT120I GGNCC 2 cut(s) 165, 466
BmiI GGNNCC 3 cut(s) 166, 468, 505
BpiI GAAGAC 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 347
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 44, 162, 163, 475, 556
Bse3DI GCAATG 2 cut(s) 48, 681
BseDI CCNNGG 1 cut(s) 347
BseLI CCNNNNNNNGG 6 cut(s) 43, 44, 162, 163, 475, 556
BseMI GCAATG 2 cut(s) 48, 681
BseMII CTCAG 2 cut(s) 606, 627
BseXI GCAGC 1 cut(s) 475
BseYI CCCAGC 2 cut(s) 37, 380
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 1 cut(s) 167
BsiHKCI CYCGRG 2 cut(s) 121, 650
BsiSI CCGG 1 cut(s) 323
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 6 cut(s) 43, 44, 162, 163, 475, 556
BsmAI GTCTC 1 cut(s) 528
BsmBI CGTCTC 1 cut(s) 528
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 167
BsoBI CYCGRG 2 cut(s) 121, 650
Bsp143I GATC 2 cut(s) 267, 646
BspACI CCGC 1 cut(s) 98
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 2 cut(s) 605, 628
BspLI GGNNCC 3 cut(s) 166, 468, 505
BsrDI GCAATG 2 cut(s) 48, 681
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 2 cut(s) 267, 646
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 172, 228
BstC8I GCNNGC 5 cut(s) 106, 134, 261, 450, 657
BstDEI CTNAG 4 cut(s) 251, 264, 592, 636
BstKTI GATC 2 cut(s) 270, 649
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 2 cut(s) 267, 646
BstMWI GCNNNNNNNGC 4 cut(s) 110, 142, 352, 614
BstNSI RCATGY 2 cut(s) 108, 136
BstV1I GCAGC 1 cut(s) 475
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
BsuRI GGCC 1 cut(s) 167
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 2 cut(s) 168, 509
Cac8I GCNNGC 5 cut(s) 106, 134, 261, 450, 657
Cfr13I GGNCC 2 cut(s) 165, 466
CseI GACGC 2 cut(s) 221, 545
Csp6I GTAC 1 cut(s) 529
CviAII CATG 2 cut(s) 105, 133
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 4 cut(s) 251, 264, 592, 636
DpnI GATC 2 cut(s) 269, 648
DpnII GATC 2 cut(s) 267, 646
EciI GGCGGA 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 466
Eco88I CYCGRG 2 cut(s) 121, 650
EcoO109I RGGNCCY 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 347
Esp3I CGTCTC 1 cut(s) 528
FaeI CATG 2 cut(s) 108, 136
FaqI GGGAC 1 cut(s) 60
FatI CATG 2 cut(s) 104, 132
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 489
Fsp4HI GCNGC 1 cut(s) 489
FspBI CTAG 2 cut(s) 285, 422
GluI GCNGC 1 cut(s) 489
GsaI CCCAGC 2 cut(s) 41, 384
HaeIII GGCC 1 cut(s) 167
HapII CCGG 1 cut(s) 323
HgaI GACGC 2 cut(s) 221, 545
Hin1II CATG 2 cut(s) 108, 136
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HindIII AAGCTT 2 cut(s) 90, 606
HinfI GANTC 5 cut(s) 33, 119, 272, 590, 695
HpaII CCGG 1 cut(s) 323
HphI GGTGA 2 cut(s) 291, 346
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 700
Hpy188III TCNNGA 5 cut(s) 369, 404, 443, 594, 650
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 3 cut(s) 457, 478, 575
HpyCH4III ACNGT 2 cut(s) 172, 228
HpyCH4V TGCA 4 cut(s) 311, 491, 514, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 142, 352, 614
HpyF3I CTNAG 4 cut(s) 251, 264, 592, 636
Hsp92II CATG 2 cut(s) 108, 136
Kzo9I GATC 2 cut(s) 267, 646
LmnI GCTCC 3 cut(s) 286, 503, 614
LpnPI CCDG 5 cut(s) 51, 336, 366, 579, 645
Lsp1109I GCAGC 1 cut(s) 475
MaeI CTAG 2 cut(s) 285, 422
MalI GATC 2 cut(s) 269, 648
MboI GATC 2 cut(s) 267, 646
MboII GAAGA 3 cut(s) 44, 397, 599
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 646
MluCI AATT 5 cut(s) 61, 206, 388, 471, 676
MlyI GAGTC 4 cut(s) 113, 281, 584, 704
MmeI TCCRAC 2 cut(s) 543, 672
MnlI CCTC 7 cut(s) 103, 249, 325, 469, 488, 517, 681
MseI TTAA 3 cut(s) 68, 432, 543
MspI CCGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 471
MwoI GCNNNNNNNGC 4 cut(s) 110, 142, 352, 614
NdeI CATATG 1 cut(s) 277
NdeII GATC 2 cut(s) 267, 646
NlaIII CATG 2 cut(s) 108, 136
NlaIV GGNNCC 3 cut(s) 166, 468, 505
NmeAIII GCCGAG 2 cut(s) 36, 169
NspI RCATGY 2 cut(s) 108, 136
PaeI GCATGC 2 cut(s) 108, 136
PaeR7I CTCGAG 1 cut(s) 650
PfeI GAWTC 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 556
PkrI GCNGC 1 cut(s) 490
PleI GAGTC 4 cut(s) 113, 280, 584, 703
PpsI GAGTC 4 cut(s) 113, 280, 584, 703
PpuMI RGGWCCY 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 466
PspFI CCCAGC 2 cut(s) 37, 380
PspN4I GGNNCC 3 cut(s) 166, 468, 505
PspPI GGNCC 2 cut(s) 165, 466
PspPPI RGGWCCY 1 cut(s) 466
PsuI RGATCY 1 cut(s) 646
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
SaqAI TTAA 3 cut(s) 68, 432, 543
SatI GCNGC 1 cut(s) 489
Sau3AI GATC 2 cut(s) 267, 646
Sau96I GGNCC 2 cut(s) 165, 466
SchI GAGTC 4 cut(s) 113, 281, 584, 704
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 1 cut(s) 466
SlaI CTCGAG 1 cut(s) 650
SmlI CTYRAG 2 cut(s) 443, 650
SmoI CTYRAG 2 cut(s) 443, 650
SphI GCATGC 2 cut(s) 108, 136
Sse9I AATT 5 cut(s) 61, 206, 388, 471, 676
SsiI CCGC 1 cut(s) 98
SspMI CTAG 2 cut(s) 285, 422
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 2 cut(s) 172, 228
TaqI TCGA 3 cut(s) 270, 370, 651
TasI AATT 5 cut(s) 61, 206, 388, 471, 676
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 3 cut(s) 68, 432, 543
Tru9I TTAA 3 cut(s) 68, 432, 543
TscAI CASTG 2 cut(s) 175, 516
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 237, 476
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 2 cut(s) 175, 516
Van91I CCANNNNNTGG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 1 cut(s) 388
XceI RCATGY 2 cut(s) 108, 136
XhoI CTCGAG 1 cut(s) 650
XspI CTAG 2 cut(s) 285, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.