Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005723

Physical Location & Seq

Genomic Coordinates Reverse (-)

16358496 .. 16359254

Gene Structure 759 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1187500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 759 bp

ATGGCAGATCACCAAAAGATTCATCCAGTACTTGATCGAGATGTTGAGGCACCACATGCAGCAGCTGAGGTACCCTTGGTACCAAGGGGAGCTTCTAAGTCGGATGGAAGTGATCCTCCTTTACGACGTACCTTTCCAGTGATGCACTCCCCACCACCCATTCACAAGAAGAGAAGTTGTTGCTGCAGGTGCCTCTGTTGGATTGTATGCCTGCTCGTGCTCCAAGTGGTGGTCATTGCGATCACTGGTGGAATTATATACCTCGTGTTCCGACCAAAGCTTCCAAAATATTCGGTGGACCAATTGCAAATAACCCAGTTCAATGTTAGCACCGATCAGAGCTTGTATTCTACTTTCGACGTGAGCATCACTGCACGAAACCCCAACAAGAAGATCGGTATTTACTATGAAGGTGGTAGCCGCTTAAGCGTGTTTTACACACGCACCAAGCTTTGTGAAGGAGCTTTGCCAAAATTCTACCAGGGTCACCGCAACACCACACAACTCCTGGTTACATTGACCGGACAAACCCAAGATGCCAGCGGCCTGCTGGCCACTCTCCAACAACAGCAGCAACAGACAGGTAATGTTCCGCTCACTCTCAGGGTCAGACAACCTGTAAAGATTAAACTCGGCAGCTTGAAGCTCCCAAAGATCTTCTTCTTGGTCAGGTGCAGGTTGCTGGTGGACAGTCTTTCGGAAAATAATGATATTAGAATTCAAAGCAGTAGTTGCAAGTTCCGGGGGATTAGGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010115 GO:0010116 GO:0010565 GO:0016020 GO:0019216 GO:0019222 GO:0019747 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033993 GO:0042221 GO:0043455 GO:0044424 GO:0044444 GO:0044464 GO:0045828 GO:0045834 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0062012 GO:0062013 GO:0065007 GO:0071944 GO:0080090 GO:0097305 GO:1901700 GO:1902074 GO:1902930 GO:1902932

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LEA_2

Protein Analysis

253

Amino Acids

28.23

Weight (kDa)

9.77

Isoelectric Point (pI)

51.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LEA_2	PF03168	123 - 207	1.2e-08	Late embryogenesis abundant protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005723)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65690 AT5G36970
fragaria_vesca	FvH4_5g39741 FvH4_5g39741
malus_domestica	MD09G1187500.v1.1
prunus_persica	Prupe.8G267700_v2.0.a1
pyrus_communis	pycom11g27770
rosa_chinensis	RchiOBHm_Chr7g0182611 RchiOBHm_Chr7g0242031
rosa_laevigata	RLG00000000514 RLG00000005116
rosa_multiflora	Rmu_co8132812.1_g000001 Rmu_co8258617.1_g000001 Rmu_sc0003559.1_g000022 Rmu_sc0005811.1_g000010 Rmu_sc0013953.1_g000002
rosa_roxburghii	Rroxscaffold_3G00218260
rosa_rugosa	Rorug06G0448600 Rorug07G0349900
rosa_samantha	Rh7AG052400 Rh7AG504800 Rh7BG051600 Rh7BG476600 Rh7CG521200 Rh7DG051600 Rh7DG490300
rosa_wichuraiana	Rw7G004250 Rw7G042670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	177
Acc36I	ACCTGC	2 cut(s)	177, 666
Acc65I	GGTACC	2 cut(s)	70, 79
AccB1I	GGYRCC	4 cut(s)	49, 70, 79, 189
AccB7I	CCANNNNNTGG	1 cut(s)	229
AccBSI	CCGCTC	1 cut(s)	595
AciI	CCGC	4 cut(s)	421, 490, 543, 593
AclWI	GGATC	1 cut(s)	107
AcoI	YGGCCR	1 cut(s)	552
AcsI	RAATTY	2 cut(s)	473, 717
AfaI	GTAC	4 cut(s)	30, 72, 81, 130
AfiI	CCNNNNNNNGG	1 cut(s)	229
AflII	CTTAAG	1 cut(s)	424
AgsI	TTSAA	3 cut(s)	322, 643, 722
AjiI	CACGTC	1 cut(s)	361
AjnI	CCWGG	2 cut(s)	480, 507
AjuI	GAANNNNNNNTTGG	2 cut(s)	76, 108
AluBI	AGCT	8 cut(s)	65, 92, 280, 342, 451, 464, 639, 646
AluI	AGCT	8 cut(s)	65, 92, 280, 342, 451, 464, 639, 646
Alw21I	GWGCWC	1 cut(s)	222
AlwI	GGATC	1 cut(s)	107
AlwNI	CAGNNNCTG	1 cut(s)	65
AoxI	GGCC	2 cut(s)	544, 552
ApeKI	GCWGC	5 cut(s)	59, 62, 183, 571, 636
ApoI	RAATTY	2 cut(s)	473, 717
Asp718I	GGTACC	2 cut(s)	70, 79
AspS9I	GGNCC	1 cut(s)	298
AsuC2I	CCSGG	1 cut(s)	743
AsuHPI	GGTGA	1 cut(s)	479
AvaII	GGWCC	1 cut(s)	298
BalI	TGGCCA	1 cut(s)	554
BanI	GGYRCC	4 cut(s)	49, 70, 79, 189
BauI	CACGAG	2 cut(s)	215, 263
Bbv12I	GWGCWC	1 cut(s)	222
BbvCI	CCTCAGC	1 cut(s)	66
BbvI	GCAGC	5 cut(s)	71, 74, 170, 583, 648
BccI	CCATC	1 cut(s)	98
BciT130I	CCWGG	2 cut(s)	482, 509
BcnI	CCSGG	1 cut(s)	743
BfmI	CTRYAG	1 cut(s)	184
BfrI	CTTAAG	1 cut(s)	424
BfuAI	ACCTGC	2 cut(s)	177, 666
BglII	AGATCT	1 cut(s)	654
BisI	GCNGC	7 cut(s)	60, 63, 184, 421, 544, 572, 637
BlsI	GCNGC	7 cut(s)	61, 64, 185, 422, 545, 573, 638
BmcAI	AGTACT	1 cut(s)	30
Bme1390I	CCNGG	3 cut(s)	482, 509, 743
Bme18I	GGWCC	1 cut(s)	298
BmgBI	CACGTC	1 cut(s)	361
BmgT120I	GGNCC	1 cut(s)	298
BmiI	GGNNCC	4 cut(s)	51, 72, 81, 191
BmrFI	CCNGG	3 cut(s)	482, 509, 743
BmrI	ACTGGG	1 cut(s)	310
BmsI	GCATC	3 cut(s)	132, 375, 526
BmuI	ACTGGG	1 cut(s)	310
Bpu10I	CCTNAGC	1 cut(s)	66
BpuMI	CCSGG	1 cut(s)	743
BsaJI	CCNNGG	4 cut(s)	75, 83, 481, 742
BsaWI	WCCGGW	1 cut(s)	521
Bsc4I	CCNNNNNNNGG	1 cut(s)	229
Bse1I	ACTGG	4 cut(s)	26, 137, 250, 316
Bse3DI	GCAATG	1 cut(s)	234
BseBI	CCWGG	2 cut(s)	482, 509
BseDI	CCNNGG	4 cut(s)	75, 83, 481, 742
BseGI	GGATG	2 cut(s)	22, 109
BseLI	CCNNNNNNNGG	1 cut(s)	229
BseMI	GCAATG	1 cut(s)	234
BseMII	CTCAG	2 cut(s)	57, 616
BseNI	ACTGG	4 cut(s)	26, 137, 250, 316
BseXI	GCAGC	5 cut(s)	71, 74, 170, 583, 648
BsgI	GTGCAG	2 cut(s)	357, 694
BshFI	GGCC	2 cut(s)	546, 554
BshNI	GGYRCC	4 cut(s)	49, 70, 79, 189
BsiHKAI	GWGCWC	1 cut(s)	222
BsiSI	CCGG	2 cut(s)	522, 742
BslI	CCNNNNNNNGG	1 cut(s)	229
BsnI	GGCC	2 cut(s)	546, 554
Bsp1286I	GDGCHC	1 cut(s)	222
Bsp143I	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
BspACI	CCGC	4 cut(s)	421, 490, 543, 593
BspANI	GGCC	2 cut(s)	546, 554
BspCNI	CTCAG	2 cut(s)	58, 615
BspLI	GGNNCC	4 cut(s)	51, 72, 81, 191
BspMAI	CTGCAG	1 cut(s)	188
BspMI	ACCTGC	2 cut(s)	177, 666
BspPI	GGATC	1 cut(s)	107
BspT107I	GGYRCC	4 cut(s)	49, 70, 79, 189
BspTI	CTTAAG	1 cut(s)	424
BsrBI	CCGCTC	1 cut(s)	595
BsrDI	GCAATG	1 cut(s)	234
BsrI	ACTGG	4 cut(s)	26, 137, 250, 316
BssECI	CCNNGG	4 cut(s)	75, 83, 481, 742
BssMI	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
BssSI	CACGAG	2 cut(s)	215, 263
BssT1I	CCWWGG	2 cut(s)	75, 83
Bst2BI	CACGAG	2 cut(s)	215, 263
Bst2UI	CCWGG	2 cut(s)	482, 509
Bst4CI	ACNGT	1 cut(s)	692
Bst6I	CTCTTC	1 cut(s)	164
BstAFI	CTTAAG	1 cut(s)	424
BstAPI	GCANNNNNTGC	2 cut(s)	56, 732
BstC8I	GCNNGC	4 cut(s)	212, 541, 548, 552
BstDEI	CTNAG	3 cut(s)	66, 96, 602
BstEII	GGTNACC	1 cut(s)	485
BstF5I	GGATG	2 cut(s)	22, 109
BstKTI	GATC	7 cut(s)	10, 37, 115, 243, 337, 396, 657
BstMBI	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
BstMWI	GCNNNNNNNGC	4 cut(s)	56, 189, 426, 732
BstNI	CCWGG	2 cut(s)	482, 509
BstNSI	RCATGY	1 cut(s)	59
BstPI	GGTNACC	1 cut(s)	485
BstSCI	CCNGG	3 cut(s)	480, 507, 741
BstSFI	CTRYAG	1 cut(s)	184
BstV1I	GCAGC	5 cut(s)	71, 74, 170, 583, 648
BstX2I	RGATCY	1 cut(s)	654
BstYI	RGATCY	1 cut(s)	654
BsuRI	GGCC	2 cut(s)	546, 554
BtrI	CACGTC	1 cut(s)	361
BtsCI	GGATG	2 cut(s)	22, 109
BtsI	GCAGTG	1 cut(s)	369
BtsIMutI	CAGTG	3 cut(s)	144, 243, 369
BveI	ACCTGC	2 cut(s)	177, 666
Cac8I	GCNNGC	4 cut(s)	212, 541, 548, 552
CaiI	CAGNNNCTG	1 cut(s)	65
Cfr13I	GGNCC	1 cut(s)	298
Csp6I	GTAC	4 cut(s)	29, 71, 80, 129
CviAII	CATG	1 cut(s)	56
CviQI	GTAC	4 cut(s)	29, 71, 80, 129
DdeI	CTNAG	3 cut(s)	66, 96, 602
DpnI	GATC	7 cut(s)	9, 36, 114, 242, 336, 395, 656
DpnII	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
EaeI	YGGCCR	1 cut(s)	552
Eam1104I	CTCTTC	1 cut(s)	164
EarI	CTCTTC	1 cut(s)	164
Eco130I	CCWWGG	2 cut(s)	75, 83
Eco47I	GGWCC	1 cut(s)	298
Eco91I	GGTNACC	1 cut(s)	485
EcoO65I	GGTNACC	1 cut(s)	485
EcoRI	GAATTC	1 cut(s)	717
EcoRII	CCWGG	2 cut(s)	480, 507
EcoT14I	CCWWGG	2 cut(s)	75, 83
ErhI	CCWWGG	2 cut(s)	75, 83
FaeI	CATG	1 cut(s)	59
FaiI	YATR	5 cut(s)	57, 208, 257, 259, 408
FalI	AAGNNNNNCTT	4 cut(s)	76, 108, 644, 676
FatI	CATG	1 cut(s)	55
Fnu4HI	GCNGC	7 cut(s)	60, 63, 184, 421, 544, 572, 637
FokI	GGATG	2 cut(s)	9, 116
Fsp4HI	GCNGC	7 cut(s)	60, 63, 184, 421, 544, 572, 637
GluI	GCNGC	7 cut(s)	60, 63, 184, 421, 544, 572, 637
HaeIII	GGCC	2 cut(s)	546, 554
HapII	CCGG	2 cut(s)	522, 742
Hin1II	CATG	1 cut(s)	59
HindIII	AAGCTT	2 cut(s)	278, 449
HinfI	GANTC	1 cut(s)	19
HpaII	CCGG	2 cut(s)	522, 742
HphI	GGTGA	1 cut(s)	479
Hpy166II	GTNNAC	2 cut(s)	298, 688
Hpy188I	TCNGA	5 cut(s)	103, 272, 339, 611, 700
Hpy188III	TCNNGA	1 cut(s)	38
Hpy8I	GTNNAC	2 cut(s)	298, 688
Hpy99I	CGWCG	2 cut(s)	129, 362
HpyAV	CCTTC	2 cut(s)	404, 452
HpyCH4III	ACNGT	1 cut(s)	692
HpyCH4IV	ACGT	2 cut(s)	127, 360
HpyCH4V	TGCA	7 cut(s)	59, 145, 186, 307, 374, 675, 735
HpyF10VI	GCNNNNNNNGC	4 cut(s)	56, 189, 426, 732
HpyF3I	CTNAG	3 cut(s)	66, 96, 602
HpySE526I	ACGT	2 cut(s)	127, 360
Hsp92II	CATG	1 cut(s)	59
KpnI	GGTACC	2 cut(s)	74, 83
Kzo9I	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
LmnI	GCTCC	4 cut(s)	89, 225, 461, 651
Lsp1109I	GCAGC	5 cut(s)	71, 74, 170, 583, 648
LweI	GCATC	3 cut(s)	132, 375, 526
MaeII	ACGT	2 cut(s)	127, 360
MaeIII	GTNAC	2 cut(s)	485, 511
MalI	GATC	7 cut(s)	9, 36, 114, 242, 336, 395, 656
MbiI	CCGCTC	1 cut(s)	595
MboI	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
MboII	GAAGA	4 cut(s)	181, 403, 649, 652
MfeI	CAATTG	1 cut(s)	302
MflI	RGATCY	1 cut(s)	654
MhlI	GDGCHC	1 cut(s)	222
MlsI	TGGCCA	1 cut(s)	554
MluCI	AATT	4 cut(s)	252, 302, 473, 717
MluNI	TGGCCA	1 cut(s)	554
MmeI	TCCRAC	4 cut(s)	81, 179, 295, 586
MnlI	CCTC	5 cut(s)	40, 61, 126, 203, 272
Mox20I	TGGCCA	1 cut(s)	554
MscI	TGGCCA	1 cut(s)	554
MseI	TTAA	2 cut(s)	425, 627
Msp20I	TGGCCA	1 cut(s)	554
MspA1I	CMGCKG	2 cut(s)	65, 543
MspCI	CTTAAG	1 cut(s)	424
MspI	CCGG	2 cut(s)	522, 742
MspR9I	CCNGG	3 cut(s)	482, 509, 743
MunI	CAATTG	1 cut(s)	302
MvaI	CCWGG	2 cut(s)	482, 509
MwoI	GCNNNNNNNGC	4 cut(s)	56, 189, 426, 732
NciI	CCSGG	1 cut(s)	743
NdeII	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
NlaIII	CATG	1 cut(s)	59
NlaIV	GGNNCC	4 cut(s)	51, 72, 81, 191
NmeAIII	GCCGAG	1 cut(s)	612
NmuCI	GTSAC	1 cut(s)	485
NspI	RCATGY	1 cut(s)	59
PaqCI	CACCTGC	1 cut(s)	177
PfeI	GAWTC	1 cut(s)	19
PflMI	CCANNNNNTGG	1 cut(s)	229
PkrI	GCNGC	7 cut(s)	61, 64, 185, 422, 545, 573, 638
Psp6I	CCWGG	2 cut(s)	480, 507
PspEI	GGTNACC	1 cut(s)	485
PspGI	CCWGG	2 cut(s)	480, 507
PspN4I	GGNNCC	4 cut(s)	51, 72, 81, 191
PspPI	GGNCC	1 cut(s)	298
PstI	CTGCAG	1 cut(s)	188
PstNI	CAGNNNCTG	1 cut(s)	65
PsuI	RGATCY	1 cut(s)	654
PvuII	CAGCTG	1 cut(s)	65
RsaI	GTAC	4 cut(s)	30, 72, 81, 130
RsaNI	GTAC	4 cut(s)	29, 71, 80, 129
SaqAI	TTAA	2 cut(s)	425, 627
SatI	GCNGC	7 cut(s)	60, 63, 184, 421, 544, 572, 637
Sau3AI	GATC	7 cut(s)	7, 34, 112, 240, 334, 393, 654
Sau96I	GGNCC	1 cut(s)	298
ScaI	AGTACT	1 cut(s)	30
ScrFI	CCNGG	3 cut(s)	482, 509, 743
SduI	GDGCHC	1 cut(s)	222
SfaNI	GCATC	3 cut(s)	132, 375, 526
SfcI	CTRYAG	1 cut(s)	184
SinI	GGWCC	1 cut(s)	298
SmlI	CTYRAG	1 cut(s)	424
SmoI	CTYRAG	1 cut(s)	424
Sse9I	AATT	4 cut(s)	252, 302, 473, 717
SsiI	CCGC	4 cut(s)	421, 490, 543, 593
SspI	AATATT	1 cut(s)	290
StyD4I	CCNGG	3 cut(s)	480, 507, 741
StyI	CCWWGG	2 cut(s)	75, 83
TaaI	ACNGT	1 cut(s)	692
TaiI	ACGT	2 cut(s)	130, 363
TaqI	TCGA	2 cut(s)	37, 357
TasI	AATT	4 cut(s)	252, 302, 473, 717
TatI	WGTACW	1 cut(s)	28
TauI	GCSGC	2 cut(s)	423, 546
TfiI	GAWTC	1 cut(s)	19
Tru1I	TTAA	2 cut(s)	425, 627
Tru9I	TTAA	2 cut(s)	425, 627
TscAI	CASTG	3 cut(s)	144, 250, 376
TseFI	GTSAC	1 cut(s)	485
TseI	GCWGC	5 cut(s)	59, 62, 183, 571, 636
Tsp45I	GTSAC	1 cut(s)	485
TspDTI	ATGAA	2 cut(s)	11, 423
TspRI	CASTG	3 cut(s)	144, 250, 376
Van91I	CCANNNNNTGG	1 cut(s)	229
Vha464I	CTTAAG	1 cut(s)	424
VpaK11BI	GGWCC	1 cut(s)	298
XapI	RAATTY	2 cut(s)	473, 717
XceI	RCATGY	1 cut(s)	59
XcmI	CCANNNNNNNNNTGG	2 cut(s)	505, 547
ZrmI	AGTACT	1 cut(s)	30

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.