MD09G1191600.v1.1

Serine threonine-protein phosphatase 7 long form homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003095
Physical Location & Seq
Reverse (-)
17019434 .. 17022402
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1191600.v1.1.491

Sequence Viewer

Length: 1497 bp
ATGGAGTCACGCATAGATCCTGGACCAGTTGATAAGTCTGTACTTTATGATCAAGAAAATCATATCTCTTCAGCGATTTGGGATGGACAGGAGCGTGGCGTGCTTAGATGTCATGAACACACTTCGATGCTTGAGCAATGGAGACTCATACCTCAACAAGTTGAGTTGGTTGAGAAGGCCGGTTTTAAACACTTCAGATCAATACCGACAATTGTTCTTGACAATTCACTAATATCGGCACTTGTTGAGAGGTGGAGGAGAGAAACAAATACATTTCATTTGCCTCTTGGAGAAATGACTGTAACGCTTGAGGATGTTGCACTTATACTTGGGCTACCAATCGATGGAAAACCTGTTATTGGTGTACCGGTGAAGACACCCAACTCGGTATGTGAAGACTTGCTTGGGAAATCACCGAAGGACCTGAATGGGGGAATGATGAAGCTAAATTGGTTGAGAGAGTGTTTTTCTAGATGTCCTGAAGATGCACCGATTGAAGAGATTGAGCGCCACACTCGTGCTTACCTCCTATATATTGTTGGTTGTACAATATTTTCCACAACCACTGGAAATAAAGTCTCTGTTACATTTCTTCCGCTGTTTGAAAAGTTTGAAGAAGCTGGAAAATATGCATGGGGTGCAGCAGCATTGGCATTCCTCTATAGAGCTCTTGGTAATGCCTCCCTTAAATCTCAAGGCACGATTAGTGGCTCTTTGACGCTACTGCAGTGCTGGAGTTACTATCACCTGAATGTTGGCCAACCGAAGTTCAATGAAGAGTCGAGCGATGGTTTTTTTCCATTTGCTCTTAGGTGGAAAGGGAGATCAACTGGATCAAGATCGAAAACCAACATAGTTGCCTACCGCAAGGCCTTGGATTCGCTTCAACCTTTTGATGTTAAGTGGTTTCCATACAAAGATTTGGATCACACAATTATACCGGAAGATATAATAAACAATCTGATTTTACGGACTTCAAAAACGATCCTGATATGCTTTGGCAAGGCAGAAAGACACCTCCCTGATCGCTGCCTCAGGCAGTTTGGCATGCTTCAACATATACCAGAGGATGTCCAGAAATGGGAGAGGAAGATTCCTGCAGTTGATCAGGGGGTAGACTTGTCAACGGATGTAGAGTTGGGGGGCAAAGGAAAAATGCGCTCAGAGATCAAGGAATGGTTAGAGCGTCGAGTCCATATTTTGGAAGGTAACGATGGTGTTGATGAAAACACGTACATGGATTGGTACGAGAGAATTACCCGCAAATATGTGGGGAGGCCTGAATCTTTAGAATCTGAGTTTCAGAGAATAGCTGCTTCTATGAGAGATATTGCGAGCATAGCTGATACATTGTCAAGAGACCAGATGAACCCCCAAGATGGGGAGTTACTTGATGAAATCAAGAAGACGGTGCATGTATGCTTGACGGATGTCGTTGGGAATTCTAAAAATGGCAGGGCTAAGAATGCCGTGAAACGTAAGAGAGAGGGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

499

Amino Acids

56.6

Weight (kDa)

6.49

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 61 - 417 2.4e-101 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 344, 1201
AccI GTMKAC 1 cut(s) 1116
AciI CCGC 3 cut(s) 596, 865, 1261
AclWI GGATC 4 cut(s) 11, 841, 933, 979
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 1 cut(s) 1441
AcuI CTGAAG 3 cut(s) 54, 178, 501
AfaI GTAC 5 cut(s) 42, 366, 547, 1235, 1247
AfiI CCNNNNNNNGG 8 cut(s) 344, 359, 430, 1081, 1201, 1379, 1380, 1381
AflIII ACRYGT 1 cut(s) 1230
AgeI ACCGGT 1 cut(s) 367
AgsI TTSAA 7 cut(s) 497, 605, 614, 772, 887, 978, 1055
AjnI CCWGG 1 cut(s) 19
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AleI CACNNNNGTG 1 cut(s) 516
AluBI AGCT 5 cut(s) 445, 620, 668, 1313, 1343
AluI AGCT 5 cut(s) 445, 620, 668, 1313, 1343
Alw21I GWGCWC 1 cut(s) 670
Alw26I GTCTC 3 cut(s) 136, 583, 1353
AlwI GGATC 4 cut(s) 11, 841, 933, 979
AoxI GGCC 4 cut(s) 177, 757, 870, 1277
ApeKI GCWGC 4 cut(s) 641, 644, 1029, 1313
ApoI RAATTY 1 cut(s) 1441
AsiGI ACCGGT 1 cut(s) 367
AspLEI GCGC 2 cut(s) 510, 1161
AspS9I GGNCC 2 cut(s) 23, 421
AsuHPI GGTGA 3 cut(s) 382, 405, 737
AvaII GGWCC 2 cut(s) 23, 421
AxyI CCTNAGG 1 cut(s) 1034
BalI TGGCCA 1 cut(s) 759
BanII GRGCYC 1 cut(s) 670
BauI CACGAG 1 cut(s) 516
BbsI GAAGAC 3 cut(s) 380, 402, 1412
Bbv12I GWGCWC 1 cut(s) 670
BbvI GCAGC 4 cut(s) 653, 656, 1016, 1300
BccI CCATC 5 cut(s) 77, 338, 782, 1208, 1373
BceAI ACGGC 1 cut(s) 1454
BciT130I CCWGG 1 cut(s) 21
BclI TGATCA 2 cut(s) 49, 1105
BcoDI GTCTC 3 cut(s) 136, 583, 1353
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 3 cut(s) 661, 725, 1098
BfoI RGCGCY 1 cut(s) 511
BisI GCNGC 4 cut(s) 642, 645, 1030, 1314
BlsI GCNGC 4 cut(s) 643, 646, 1031, 1315
Bme1390I CCNGG 1 cut(s) 21
Bme18I GGWCC 2 cut(s) 23, 421
BmgT120I GGNCC 2 cut(s) 23, 421
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 2 cut(s) 117, 475
BoxI GACNNNNGTC 1 cut(s) 1430
BpiI GAAGAC 3 cut(s) 380, 402, 1412
BpmI CTGGAG 1 cut(s) 754
BpuEI CTTGAG 3 cut(s) 152, 329, 678
Bsa29I ATCGAT 1 cut(s) 342
BsaAI YACGTR 1 cut(s) 1233
BsaBI GATNNNNATC 2 cut(s) 838, 924
BsaI GGTCTC 1 cut(s) 1353
BsaJI CCNNGG 1 cut(s) 873
BsaWI WCCGGW 2 cut(s) 367, 940
Bsc4I CCNNNNNNNGG 8 cut(s) 344, 359, 430, 1081, 1201, 1379, 1380, 1381
Bse118I RCCGGY 2 cut(s) 179, 367
Bse1I ACTGG 3 cut(s) 26, 571, 835
Bse21I CCTNAGG 1 cut(s) 1034
Bse3DI GCAATG 1 cut(s) 143
Bse8I GATNNNNATC 2 cut(s) 838, 924
BseBI CCWGG 1 cut(s) 21
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 873
BseGI GGATG 5 cut(s) 88, 319, 1075, 1135, 1435
BseJI GATNNNNATC 2 cut(s) 838, 924
BseLI CCNNNNNNNGG 8 cut(s) 344, 359, 430, 1081, 1201, 1379, 1380, 1381
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 3 cut(s) 1048, 1176, 1287
BseNI ACTGG 3 cut(s) 26, 571, 835
BseRI GAGGAG 1 cut(s) 271
BseXI GCAGC 4 cut(s) 653, 656, 1016, 1300
BsgI GTGCAG 1 cut(s) 660
BshFI GGCC 4 cut(s) 179, 759, 872, 1279
BshTI ACCGGT 1 cut(s) 367
BshVI ATCGAT 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 670
BsiSI CCGG 3 cut(s) 180, 368, 941
BslI CCNNNNNNNGG 8 cut(s) 344, 359, 430, 1081, 1201, 1379, 1380, 1381
BsmAI GTCTC 3 cut(s) 136, 583, 1353
BsmI GAATGC 2 cut(s) 653, 1471
BsnI GGCC 4 cut(s) 179, 759, 872, 1279
Bso31I GGTCTC 1 cut(s) 1353
Bsp1286I GDGCHC 1 cut(s) 670
Bsp1407I TGTACA 1 cut(s) 545
BspACI CCGC 3 cut(s) 596, 865, 1261
BspANI GGCC 4 cut(s) 179, 759, 872, 1279
BspCNI CTCAG 3 cut(s) 1047, 1175, 1288
BspDI ATCGAT 1 cut(s) 342
BspHI TCATGA 1 cut(s) 112
BspMAI CTGCAG 2 cut(s) 729, 1102
BspPI GGATC 4 cut(s) 11, 841, 933, 979
BspTNI GGTCTC 1 cut(s) 1353
BsrDI GCAATG 1 cut(s) 143
BsrFI RCCGGY 2 cut(s) 179, 367
BsrGI TGTACA 1 cut(s) 545
BsrI ACTGG 3 cut(s) 26, 571, 835
BssAI RCCGGY 2 cut(s) 179, 367
BssECI CCNNGG 1 cut(s) 873
BssSI CACGAG 1 cut(s) 516
BssT1I CCWWGG 1 cut(s) 873
Bst2BI CACGAG 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 301, 1411
Bst6I CTCTTC 3 cut(s) 73, 492, 771
BstAPI GCANNNNNTGC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 1233
BstC8I GCNNGC 3 cut(s) 101, 1049, 1336
BstDEI CTNAG 6 cut(s) 104, 809, 1034, 1162, 1296, 1461
BstF5I GGATG 5 cut(s) 88, 319, 1075, 1135, 1435
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 2 cut(s) 510, 1161
BstMAI GTCTC 3 cut(s) 136, 583, 1353
BstMWI GCNNNNNNNGC 5 cut(s) 100, 638, 650, 1340, 1466
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 2 cut(s) 1051, 1418
BstPAI GACNNNNGTC 1 cut(s) 1430
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 3 cut(s) 661, 725, 1098
BstV1I GCAGC 4 cut(s) 653, 656, 1016, 1300
BstV2I GAAGAC 3 cut(s) 380, 402, 1412
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 342
Bsu36I CCTNAGG 1 cut(s) 1034
BsuRI GGCC 4 cut(s) 179, 759, 872, 1279
BsuTUI ATCGAT 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 5 cut(s) 88, 319, 1075, 1135, 1435
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 2 cut(s) 564, 734
Cac8I GCNNGC 3 cut(s) 101, 1049, 1336
CciI TCATGA 1 cut(s) 112
CfoI GCGC 2 cut(s) 510, 1161
Cfr10I RCCGGY 2 cut(s) 179, 367
Cfr13I GGNCC 2 cut(s) 23, 421
ClaI ATCGAT 1 cut(s) 342
CseI GACGC 2 cut(s) 727, 1175
Csp6I GTAC 5 cut(s) 41, 365, 546, 1234, 1246
CspAI ACCGGT 1 cut(s) 367
CviAII CATG 5 cut(s) 113, 633, 1048, 1237, 1415
CviQI GTAC 5 cut(s) 41, 365, 546, 1234, 1246
DdeI CTNAG 6 cut(s) 104, 809, 1034, 1162, 1296, 1461
DraI TTTAAA 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 757
Eam1104I CTCTTC 3 cut(s) 73, 492, 771
EarI CTCTTC 3 cut(s) 73, 492, 771
Ecl136II GAGCTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 873
Eco147I AGGCCT 2 cut(s) 872, 1279
Eco24I GRGCYC 1 cut(s) 670
Eco31I GGTCTC 1 cut(s) 1353
Eco47I GGWCC 2 cut(s) 23, 421
Eco53kI GAGCTC 1 cut(s) 668
Eco57I CTGAAG 3 cut(s) 54, 178, 501
Eco81I CCTNAGG 1 cut(s) 1034
EcoICRI GAGCTC 1 cut(s) 668
EcoO109I RGGNCCY 1 cut(s) 421
EcoRI GAATTC 1 cut(s) 1441
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 873
EcoT22I ATGCAT 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 5 cut(s) 116, 636, 1051, 1240, 1418
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FatI CATG 5 cut(s) 112, 632, 1047, 1236, 1414
FauI CCCGC 1 cut(s) 1268
FbaI TGATCA 2 cut(s) 49, 1105
FblI GTMKAC 1 cut(s) 1116
Fnu4HI GCNGC 4 cut(s) 642, 645, 1030, 1314
FokI GGATG 5 cut(s) 95, 326, 1082, 1142, 1442
FriOI GRGCYC 1 cut(s) 670
Fsp4HI GCNGC 4 cut(s) 642, 645, 1030, 1314
FspBI CTAG 1 cut(s) 471
GlaI GCGC 2 cut(s) 509, 1160
GluI GCNGC 4 cut(s) 642, 645, 1030, 1314
GsuI CTGGAG 1 cut(s) 754
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 4 cut(s) 179, 759, 872, 1279
HapII CCGG 3 cut(s) 180, 368, 941
HgaI GACGC 2 cut(s) 727, 1175
HhaI GCGC 2 cut(s) 510, 1161
Hin1II CATG 5 cut(s) 116, 636, 1051, 1240, 1418
Hin6I GCGC 2 cut(s) 508, 1159
HinP1I GCGC 2 cut(s) 508, 1159
HincII GTYRAC 1 cut(s) 1125
HindII GTYRAC 1 cut(s) 1125
HinfI GANTC 8 cut(s) 5, 144, 779, 878, 1093, 1191, 1283, 1292
HpaII CCGG 3 cut(s) 180, 368, 941
HphI GGTGA 3 cut(s) 382, 405, 737
Hpy166II GTNNAC 3 cut(s) 365, 1117, 1125
Hpy188I TCNGA 5 cut(s) 197, 963, 1165, 1297, 1305
Hpy8I GTNNAC 3 cut(s) 365, 1117, 1125
Hpy99I CGWCG 1 cut(s) 1191
HpyAV CCTTC 3 cut(s) 169, 412, 1199
HpyCH4III ACNGT 2 cut(s) 301, 1411
HpyCH4IV ACGT 2 cut(s) 1232, 1477
HpyCH4V TGCA 7 cut(s) 320, 488, 632, 641, 727, 1100, 1414
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 638, 650, 1340, 1466
HpyF3I CTNAG 6 cut(s) 104, 809, 1034, 1162, 1296, 1461
HpySE526I ACGT 2 cut(s) 1232, 1477
Hsp92II CATG 5 cut(s) 116, 636, 1051, 1240, 1418
HspAI GCGC 2 cut(s) 508, 1159
Ksp22I TGATCA 2 cut(s) 49, 1105
LmnI GCTCC 1 cut(s) 91
Lsp1109I GCAGC 4 cut(s) 653, 656, 1016, 1300
LweI GCATC 2 cut(s) 117, 475
MaeI CTAG 1 cut(s) 471
MaeII ACGT 2 cut(s) 1232, 1477
MaeIII GTNAC 6 cut(s) 6, 301, 583, 737, 1208, 1386
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 670
MlsI TGGCCA 1 cut(s) 759
MluCI AATT 6 cut(s) 210, 223, 448, 933, 1254, 1441
MluNI TGGCCA 1 cut(s) 759
MlyI GAGTC 4 cut(s) 14, 138, 788, 1200
Mox20I TGGCCA 1 cut(s) 759
Mph1103I ATGCAT 1 cut(s) 634
MscI TGGCCA 1 cut(s) 759
MseI TTAA 3 cut(s) 186, 687, 900
MslI CAYNNNNRTG 4 cut(s) 125, 516, 750, 1235
Msp20I TGGCCA 1 cut(s) 759
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 3 cut(s) 180, 368, 941
MspR9I CCNGG 1 cut(s) 21
MunI CAATTG 1 cut(s) 210
Mva1269I GAATGC 2 cut(s) 653, 1471
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 5 cut(s) 100, 638, 650, 1340, 1466
NlaIII CATG 5 cut(s) 116, 636, 1051, 1240, 1418
NmuCI GTSAC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 634
NspI RCATGY 2 cut(s) 1051, 1418
OliI CACNNNNGTG 1 cut(s) 516
PaeI GCATGC 1 cut(s) 1051
PagI TCATGA 1 cut(s) 112
PceI AGGCCT 2 cut(s) 872, 1279
PctI GAATGC 2 cut(s) 653, 1471
PfeI GAWTC 4 cut(s) 878, 1093, 1283, 1292
PflMI CCANNNNNTGG 2 cut(s) 344, 1201
PfoI TCCNGGA 1 cut(s) 19
PinAI ACCGGT 1 cut(s) 367
PkrI GCNGC 4 cut(s) 643, 646, 1031, 1315
PleI GAGTC 4 cut(s) 13, 138, 787, 1199
PpsI GAGTC 4 cut(s) 13, 138, 787, 1199
Ppu21I YACGTR 1 cut(s) 1233
PpuMI RGGWCCY 1 cut(s) 421
PshAI GACNNNNGTC 1 cut(s) 1430
Psp124BI GAGCTC 1 cut(s) 670
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
PspPI GGNCC 2 cut(s) 23, 421
PspPPI RGGWCCY 1 cut(s) 421
PstI CTGCAG 2 cut(s) 729, 1102
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 5 cut(s) 42, 366, 547, 1235, 1247
RsaNI GTAC 5 cut(s) 41, 365, 546, 1234, 1246
RseI CAYNNNNRTG 4 cut(s) 125, 516, 750, 1235
SacI GAGCTC 1 cut(s) 670
SaqAI TTAA 3 cut(s) 186, 687, 900
SatI GCNGC 4 cut(s) 642, 645, 1030, 1314
Sau96I GGNCC 2 cut(s) 23, 421
SchI GAGTC 4 cut(s) 14, 138, 788, 1200
ScrFI CCNGG 1 cut(s) 21
SduI GDGCHC 1 cut(s) 670
SfaNI GCATC 2 cut(s) 117, 475
SfcI CTRYAG 3 cut(s) 661, 725, 1098
SinI GGWCC 2 cut(s) 23, 421
SmiMI CAYNNNNRTG 4 cut(s) 125, 516, 750, 1235
SmlI CTYRAG 3 cut(s) 131, 308, 693
SmoI CTYRAG 3 cut(s) 131, 308, 693
SphI GCATGC 1 cut(s) 1051
Sse9I AATT 6 cut(s) 210, 223, 448, 933, 1254, 1441
SseBI AGGCCT 2 cut(s) 872, 1279
SsiI CCGC 3 cut(s) 596, 865, 1261
SspI AATATT 1 cut(s) 552
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 670
StuI AGGCCT 2 cut(s) 872, 1279
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 2 cut(s) 301, 1411
TaiI ACGT 2 cut(s) 1235, 1480
TaqI TCGA 5 cut(s) 125, 342, 782, 842, 1189
TasI AATT 6 cut(s) 210, 223, 448, 933, 1254, 1441
TatI WGTACW 2 cut(s) 40, 545
TfiI GAWTC 4 cut(s) 878, 1093, 1283, 1292
Tru1I TTAA 3 cut(s) 186, 687, 900
Tru9I TTAA 3 cut(s) 186, 687, 900
TscAI CASTG 2 cut(s) 571, 734
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 4 cut(s) 641, 644, 1029, 1313
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 7 cut(s) 129, 266, 455, 789, 1239, 1382, 1410
TspGWI ACGGA 3 cut(s) 985, 1142, 1442
TspRI CASTG 2 cut(s) 571, 734
Van91I CCANNNNNTGG 2 cut(s) 344, 1201
VpaK11BI GGWCC 2 cut(s) 23, 421
XapI RAATTY 1 cut(s) 1441
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 2 cut(s) 1051, 1418
XmiI GTMKAC 1 cut(s) 1116
XspI CTAG 1 cut(s) 471
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.