MD09G1221000.v1.1

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007344
Physical Location & Seq
Reverse (-)
25413128 .. 25422362
9235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1221000.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGTCTTTAAGGTCTGTAAAAATGTCGGAGGACGTCTGGCTGACGTGCCTTACACACGCATTGTCCACCGAGACGGAGGAGATCATGGGCCTCCTGCTTGGTGACATCGAGAACTCTGTAAATGGCAGTGTGACAGCACTAATTTGGGGAGCATCACCTCAGACACGATCTGATCGGAGGAAGGATCGTGTGGAGACTAATCCTGAACAGTTGGCTGCTGCATCAGCCCAGGCCGAAATATCCTTTGTAATGACACTGTCAACTGGAAGAACAACTAGAGTGATTGGGTGGTACCATTCACATCCTCATATTACAGTTCTTCCATCTCATGTTGACGTGCGGACGCAGGCAATGTATCAACTTCTAGATTCTGGGTTTATTGGGCTCATATTTTCCTGCTTTAGTGAAGATATAAACAAGGTTGGGCGAATCCAAGTCATTGCTTTTCAGTCCTCGGATGGGAAGCAGCATCACATGTCAAGACCTATATCTTTATCTCCTGTACATAGAAGTCCAGTTATAGATGTTGAATCGTCTTTAAGTACCTCAGAAAATGCACCAGTGAGATCCACTTTAACTAGAGCAGGGAGTCCTGAACAAGACACAGCTGATTCAAGAACTTCTGGAACTATAAAGGGTGGGGGGAGGTCTTCAGACCTGGGGCTTTTCTTTGCCAATGCTGATGCAAGTCATCCAGGAAGAGTTGGAGGAAACTACCAAACTGACACTTCAAGCAACATCATTGGCGATATAGATCCCATGGATATGTCAGAGAGTATGCAAGAAGCAATGCACCGTTCTAACTTGGATATGAGTGGTGCTGAGTATGCCAGGAAAGAAGTTCCTCTTCATGTTTTACCGACCTCATCTCTTGTTAAGCTCGATTCACCTTTAATGTCATTCGCCGAATTGCAACGAGTACTGTATGAAGAGGAGCGAGCGGCTTATAACCAAGCAATCTTGCAAAATATGAGGGATGGGAAAGTGCATCCTCTTACTTTCATACATCACACGTCAATGTACCAGTCTTCCATGTGCAAATTAATCGAATATTGTTTGAGTCCTGCCATAAGTGCACTGCAATGTCGGTTAAGAGAGAATGAAACTCGGTTATCAGTGCTCACCGATCAAGTGAAGATTTTGGAAACTGAGACGCATAGAATAGCTGAGACAAATTCAGGATCTCCTCGTCCAGTTTCATCTCCTGTACTCCGTGGGAGTGCTCCGTTTGGTCACAGGGAAATGTTTAGTCCTGCTGAATCTGTCAGTGCAAGGAGTCCAGCTGGTTCTGGCAGTAGGAGCCGAAAAGGTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

439

Amino Acids

47.92

Weight (kDa)

6.15

Isoelectric Point (pI)

70.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 2 - 121 7.8e-18 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Prok-JAB PF14464 10 - 116 1.7e-07 Prokaryotic homologs of the JAB domain
BRCC36_C PF18110 298 - 380 2.5e-21 BRCC36 C-terminal helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
AatII GACGTC 1 cut(s) 36
Acc65I GGTACC 1 cut(s) 291
AccB1I GGYRCC 1 cut(s) 291
AccBSI CCGCTC 1 cut(s) 941
AciI CCGC 2 cut(s) 340, 941
AclWI GGATC 4 cut(s) 192, 561, 749, 1189
AcsI RAATTY 1 cut(s) 1174
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 6 cut(s) 293, 504, 544, 921, 1022, 1209
AfiI CCNNNNNNNGG 1 cut(s) 459
AflIII ACRYGT 2 cut(s) 474, 1011
AgsI TTSAA 3 cut(s) 530, 615, 732
AjiI CACGTC 3 cut(s) 45, 337, 1014
AjnI CCWGG 4 cut(s) 228, 657, 694, 830
AluBI AGCT 4 cut(s) 608, 880, 1166, 1283
AluI AGCT 4 cut(s) 608, 880, 1166, 1283
Alw21I GWGCWC 3 cut(s) 1078, 1122, 1225
Alw26I GTCTC 4 cut(s) 65, 188, 1145, 1163
Alw44I GTGCAC 1 cut(s) 1074
AlwI GGATC 4 cut(s) 192, 561, 749, 1189
AoxI GGCC 2 cut(s) 88, 231
ApaLI GTGCAC 1 cut(s) 1074
ApeKI GCWGC 3 cut(s) 215, 218, 466
ApoI RAATTY 1 cut(s) 1174
AseI ATTAAT 1 cut(s) 1043
Asp700I GAANNNNTTC 1 cut(s) 1309
Asp718I GGTACC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 88
AsuHPI GGTGA 4 cut(s) 113, 147, 879, 1114
BaeGI GKGCMC 1 cut(s) 1078
BanI GGYRCC 1 cut(s) 291
BanII GRGCYC 1 cut(s) 387
BbsI GAAGAC 2 cut(s) 642, 1020
Bbv12I GWGCWC 3 cut(s) 1078, 1122, 1225
BbvI GCAGC 3 cut(s) 202, 205, 478
BccI CCATC 3 cut(s) 331, 452, 971
BcgI CGANNNNNNTGC 2 cut(s) 1027, 1061
BciT130I CCWGG 4 cut(s) 230, 659, 696, 832
BcoDI GTCTC 4 cut(s) 65, 188, 1145, 1163
BfaI CTAG 3 cut(s) 276, 365, 579
BisI GCNGC 4 cut(s) 216, 219, 467, 942
BlsI GCNGC 4 cut(s) 217, 220, 468, 943
BmcAI AGTACT 1 cut(s) 921
Bme1390I CCNGG 4 cut(s) 230, 659, 696, 832
BmgBI CACGTC 3 cut(s) 45, 337, 1014
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 2 cut(s) 293, 1301
BmrFI CCNGG 4 cut(s) 230, 659, 696, 832
BmsI GCATC 5 cut(s) 161, 230, 478, 673, 997
BpiI GAAGAC 2 cut(s) 642, 1020
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 5 cut(s) 228, 453, 658, 759, 1213
Bsc4I CCNNNNNNNGG 1 cut(s) 459
Bse1I ACTGG 5 cut(s) 268, 515, 560, 1024, 1193
Bse3DI GCAATG 4 cut(s) 357, 438, 795, 1088
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 4 cut(s) 230, 659, 696, 832
BseDI CCNNGG 5 cut(s) 228, 453, 658, 759, 1213
BseGI GGATG 5 cut(s) 301, 463, 691, 982, 988
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 1 cut(s) 459
BseMI GCAATG 4 cut(s) 357, 438, 795, 1088
BseMII CTCAG 5 cut(s) 173, 561, 813, 1140, 1158
BseNI ACTGG 5 cut(s) 268, 515, 560, 1024, 1193
BseRI GAGGAG 3 cut(s) 92, 947, 1176
BseSI GKGCMC 1 cut(s) 1078
BseXI GCAGC 3 cut(s) 202, 205, 478
BshFI GGCC 2 cut(s) 90, 233
BshNI GGYRCC 1 cut(s) 291
BsiHKAI GWGCWC 3 cut(s) 1078, 1122, 1225
BslI CCNNNNNNNGG 1 cut(s) 459
BsmAI GTCTC 4 cut(s) 65, 188, 1145, 1163
BsmBI CGTCTC 2 cut(s) 65, 1145
BsnI GGCC 2 cut(s) 90, 233
Bsp1286I GDGCHC 4 cut(s) 387, 1078, 1122, 1225
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
Bsp19I CCATGG 1 cut(s) 759
BspACI CCGC 2 cut(s) 340, 941
BspANI GGCC 2 cut(s) 90, 233
BspCNI CTCAG 5 cut(s) 172, 560, 814, 1141, 1159
BspLI GGNNCC 2 cut(s) 293, 1301
BspPI GGATC 4 cut(s) 192, 561, 749, 1189
BspT107I GGYRCC 1 cut(s) 291
BsrBI CCGCTC 1 cut(s) 941
BsrDI GCAATG 4 cut(s) 357, 438, 795, 1088
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 5 cut(s) 268, 515, 560, 1024, 1193
BssECI CCNNGG 5 cut(s) 228, 453, 658, 759, 1213
BssMI GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 759
Bst2UI CCWGG 4 cut(s) 230, 659, 696, 832
Bst4CI ACNGT 5 cut(s) 210, 258, 316, 797, 924
Bst6I CTCTTC 3 cut(s) 694, 852, 924
BstACI GRCGYC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 502
BstC8I GCNNGC 2 cut(s) 348, 939
BstDEI CTNAG 5 cut(s) 159, 547, 822, 1149, 1167
BstDSI CCRYGG 2 cut(s) 759, 1213
BstF5I GGATG 5 cut(s) 301, 463, 691, 982, 988
BstKTI GATC 8 cut(s) 84, 170, 175, 187, 569, 757, 1129, 1184
BstMAI GTCTC 4 cut(s) 65, 188, 1145, 1163
BstMBI GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
BstMWI GCNNNNNNNGC 4 cut(s) 224, 827, 1073, 1299
BstNI CCWGG 4 cut(s) 230, 659, 696, 832
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 4 cut(s) 228, 657, 694, 830
BstSLI GKGCMC 1 cut(s) 1078
BstV1I GCAGC 3 cut(s) 202, 205, 478
BstV2I GAAGAC 2 cut(s) 642, 1020
BstX2I RGATCY 3 cut(s) 566, 754, 1181
BstYI RGATCY 3 cut(s) 566, 754, 1181
BsuRI GGCC 2 cut(s) 90, 233
BtgI CCRYGG 2 cut(s) 759, 1213
BtrI CACGTC 3 cut(s) 45, 337, 1014
BtsCI GGATG 5 cut(s) 301, 463, 691, 982, 988
BtsI GCAGTG 2 cut(s) 133, 1076
BtsIMutI CAGTG 6 cut(s) 133, 254, 567, 1076, 1122, 1273
Cac8I GCNNGC 2 cut(s) 348, 939
Cfr13I GGNCC 1 cut(s) 88
CseI GACGC 2 cut(s) 352, 1162
Csp6I GTAC 6 cut(s) 292, 503, 543, 920, 1021, 1208
CviAII CATG 6 cut(s) 85, 329, 475, 760, 851, 1033
CviQI GTAC 6 cut(s) 292, 503, 543, 920, 1021, 1208
DdeI CTNAG 5 cut(s) 159, 547, 822, 1149, 1167
DpnI GATC 8 cut(s) 83, 169, 174, 186, 568, 756, 1128, 1183
DpnII GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
Eam1104I CTCTTC 3 cut(s) 694, 852, 924
EarI CTCTTC 3 cut(s) 694, 852, 924
Eco130I CCWWGG 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 636
EcoRII CCWGG 4 cut(s) 228, 657, 694, 830
EcoT14I CCWWGG 1 cut(s) 759
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 759
Esp3I CGTCTC 2 cut(s) 65, 1145
FaeI CATG 6 cut(s) 88, 332, 478, 763, 854, 1036
FalI AAGNNNNNCTT 2 cut(s) 831, 863
FatI CATG 6 cut(s) 84, 328, 474, 759, 850, 1032
Fnu4HI GCNGC 4 cut(s) 216, 219, 467, 942
FokI GGATG 5 cut(s) 288, 470, 678, 975, 989
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 4 cut(s) 216, 219, 467, 942
FspBI CTAG 3 cut(s) 276, 365, 579
GluI GCNGC 4 cut(s) 216, 219, 467, 942
HaeIII GGCC 2 cut(s) 90, 233
HgaI GACGC 2 cut(s) 352, 1162
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 6 cut(s) 88, 332, 478, 763, 854, 1036
HincII GTYRAC 2 cut(s) 261, 334
HindII GTYRAC 2 cut(s) 261, 334
HinfI GANTC 9 cut(s) 368, 429, 530, 589, 611, 884, 1060, 1259, 1276
HphI GGTGA 4 cut(s) 113, 147, 879, 1114
Hpy166II GTNNAC 4 cut(s) 66, 261, 334, 1076
Hpy188I TCNGA 8 cut(s) 28, 162, 172, 177, 457, 550, 655, 772
Hpy188III TCNNGA 8 cut(s) 109, 203, 365, 480, 593, 615, 624, 1179
Hpy8I GTNNAC 4 cut(s) 66, 261, 334, 1076
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 5 cut(s) 210, 258, 316, 797, 924
HpyCH4IV ACGT 4 cut(s) 33, 44, 336, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 827, 1073, 1299
HpyF3I CTNAG 5 cut(s) 159, 547, 822, 1149, 1167
HpySE526I ACGT 4 cut(s) 33, 44, 336, 1013
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 6 cut(s) 88, 332, 478, 763, 854, 1036
KpnI GGTACC 1 cut(s) 295
Kzo9I GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
LmnI GCTCC 4 cut(s) 149, 934, 1228, 1299
Lsp1109I GCAGC 3 cut(s) 202, 205, 478
LweI GCATC 5 cut(s) 161, 230, 478, 673, 997
MaeI CTAG 3 cut(s) 276, 365, 579
MaeII ACGT 4 cut(s) 33, 44, 336, 1013
MaeIII GTNAC 3 cut(s) 101, 130, 1232
MalI GATC 8 cut(s) 83, 169, 174, 186, 568, 756, 1128, 1183
MbiI CCGCTC 1 cut(s) 941
MboI GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
MboII GAAGA 9 cut(s) 279, 311, 419, 642, 711, 839, 941, 1020, 1147
MflI RGATCY 3 cut(s) 566, 754, 1181
MhlI GDGCHC 4 cut(s) 387, 1078, 1122, 1225
MluCI AATT 4 cut(s) 141, 908, 1040, 1174
MlyI GAGTC 3 cut(s) 598, 1069, 1285
MmeI TCCRAC 2 cut(s) 6, 685
MroXI GAANNNNTTC 1 cut(s) 1309
MseI TTAA 7 cut(s) 8, 539, 575, 876, 893, 1043, 1091
MslI CAYNNNNRTG 3 cut(s) 764, 1016, 1081
MspA1I CMGCKG 2 cut(s) 608, 1283
MspR9I CCNGG 4 cut(s) 230, 659, 696, 832
MvaI CCWGG 4 cut(s) 230, 659, 696, 832
MwoI GCNNNNNNNGC 4 cut(s) 224, 827, 1073, 1299
NcoI CCATGG 1 cut(s) 759
NdeII GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
NlaIII CATG 6 cut(s) 88, 332, 478, 763, 854, 1036
NlaIV GGNNCC 2 cut(s) 293, 1301
NmuCI GTSAC 3 cut(s) 101, 130, 1232
NspI RCATGY 1 cut(s) 478
PciI ACATGT 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 1309
PfeI GAWTC 6 cut(s) 368, 429, 530, 611, 884, 1259
PflFI GACNNNGTC 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 694
PkrI GCNGC 4 cut(s) 217, 220, 468, 943
PleI GAGTC 3 cut(s) 597, 1068, 1284
PpsI GAGTC 3 cut(s) 597, 1068, 1284
PscI ACATGT 1 cut(s) 474
PshBI ATTAAT 1 cut(s) 1043
PsiI TTATAA 1 cut(s) 948
Psp6I CCWGG 4 cut(s) 228, 657, 694, 830
PspGI CCWGG 4 cut(s) 228, 657, 694, 830
PspN4I GGNNCC 2 cut(s) 293, 1301
PspPI GGNCC 1 cut(s) 88
PsuI RGATCY 3 cut(s) 566, 754, 1181
PsyI GACNNNGTC 1 cut(s) 256
PvuII CAGCTG 2 cut(s) 608, 1283
RsaI GTAC 6 cut(s) 293, 504, 544, 921, 1022, 1209
RsaNI GTAC 6 cut(s) 292, 503, 543, 920, 1021, 1208
RseI CAYNNNNRTG 3 cut(s) 764, 1016, 1081
SaqAI TTAA 7 cut(s) 8, 539, 575, 876, 893, 1043, 1091
SatI GCNGC 4 cut(s) 216, 219, 467, 942
Sau3AI GATC 8 cut(s) 81, 167, 172, 184, 566, 754, 1126, 1181
Sau96I GGNCC 1 cut(s) 88
ScaI AGTACT 1 cut(s) 921
SchI GAGTC 3 cut(s) 598, 1069, 1285
ScrFI CCNGG 4 cut(s) 230, 659, 696, 832
SduI GDGCHC 4 cut(s) 387, 1078, 1122, 1225
SfaNI GCATC 5 cut(s) 161, 230, 478, 673, 997
SmiMI CAYNNNNRTG 3 cut(s) 764, 1016, 1081
Sse9I AATT 4 cut(s) 141, 908, 1040, 1174
SsiI CCGC 2 cut(s) 340, 941
SspI AATATT 1 cut(s) 1052
SspMI CTAG 3 cut(s) 276, 365, 579
StyD4I CCNGG 4 cut(s) 228, 657, 694, 830
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 5 cut(s) 210, 258, 316, 797, 924
TaiI ACGT 4 cut(s) 36, 47, 339, 1016
TaqI TCGA 3 cut(s) 108, 882, 1047
TasI AATT 4 cut(s) 141, 908, 1040, 1174
TatI WGTACW 3 cut(s) 502, 919, 1207
TauI GCSGC 1 cut(s) 944
TfiI GAWTC 6 cut(s) 368, 429, 530, 611, 884, 1259
Tru1I TTAA 7 cut(s) 8, 539, 575, 876, 893, 1043, 1091
Tru9I TTAA 7 cut(s) 8, 539, 575, 876, 893, 1043, 1091
TscAI CASTG 6 cut(s) 133, 261, 567, 1083, 1122, 1273
TseFI GTSAC 3 cut(s) 101, 130, 1232
TseI GCWGC 3 cut(s) 215, 218, 466
Tsp45I GTSAC 3 cut(s) 101, 130, 1232
TspDTI ATGAA 5 cut(s) 839, 942, 991, 1116, 1188
TspGWI ACGGA 3 cut(s) 89, 1202, 1215
TspRI CASTG 6 cut(s) 133, 261, 567, 1083, 1122, 1273
Tth111I GACNNNGTC 1 cut(s) 256
VneI GTGCAC 1 cut(s) 1074
VspI ATTAAT 1 cut(s) 1043
XapI RAATTY 1 cut(s) 1174
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 1 cut(s) 478
XmnI GAANNNNTTC 1 cut(s) 1309
XspI CTAG 3 cut(s) 276, 365, 579
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.