Rorug02G0332100

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002834
Physical Location & Seq
Reverse (-)
41175044 .. 41177326
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0332100.1

Sequence Viewer

Length: 783 bp
ATGGCAACTCTGTCTCAACCAACGTTGTTTCAGGAGAAGGAAAATCTTGATCTCAATGGTAATCTTTCATTTCCTTCTTCTTTTCATGTTTCAAATTGTTCTTCTAACCCTAATTTTGCCAGAGCACTGAGTGTCAATTCCAATTCCAGAAAGCTCCACTTGGTTCTTGATCTTGATCACACACTCCTCCACACCACTCAACTCTGTAATCTGACACCTGAAGAAGACTATCTCAAGATCGGAACCCATCCTCATCGAGATGTTTTCGTGCTTGAAACTGTGATCACCAAGCTCAGGCCCTTTGTCAGGAGATTCTTGGATGAAGCCAGCAAGATGTTTGAGCTGTATATATACACAAAGGGTAACCGGTACTATGCGGGTCTAATGGCTGCCCTGCTTGATCCTAGGAAGGAGTATTTCACTTCCGTTTCTAGAGTGATATCGTGCGAGGATCACGGAATCGTGGGGCGATATAAGAGTTTGGATGCTGTGGTTGGCTGCAATTATTCTAATGTTTTGATCCTTGATGATACAGAAGAGGCATGGACAAAGGAAAGCCGGGACAATCTAATCGTTGTCGACAAGTATTGTTTCTTTAGGCACAAAATGGGCCTAGCTAAATGCAAGTCTTATGCTGAGTTAAAGACTGATGAATGTGGATATCTGGCAGCACTTCTTGAAGTTCTTAAGGTAATCAACCGTATCTTCTGTGATGAACTAGCCAGAAATCCTTCTGGTGTAGATGTGAGGGATGTGTTACAACTGTTTGGAAACAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

260

Amino Acids

29.63

Weight (kDa)

6.25

Isoelectric Point (pI)

29.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 53 - 211 2.1e-24 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 579
AciI CCGC 1 cut(s) 377
AclI AACGTT 1 cut(s) 23
AclWI GGATC 3 cut(s) 395, 459, 514
AcuI CTGAAG 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 131
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 2 cut(s) 294, 306
AflII CTTAAG 1 cut(s) 686
AgeI ACCGGT 1 cut(s) 366
AgsI TTSAA 3 cut(s) 93, 275, 680
AluBI AGCT 4 cut(s) 154, 292, 343, 617
AluI AGCT 4 cut(s) 154, 292, 343, 617
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 3 cut(s) 395, 459, 514
AoxI GGCC 2 cut(s) 296, 610
ApeKI GCWGC 3 cut(s) 389, 498, 668
AsiGI ACCGGT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 730
AspA2I CCTAGG 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 297, 610
AsuC2I CCSGG 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 277
AvrII CCTAGG 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 3 cut(s) 376, 485, 680
BccI CCATC 1 cut(s) 255
BclI TGATCA 2 cut(s) 175, 282
BcnI CCSGG 1 cut(s) 560
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 4 cut(s) 405, 432, 614, 719
BfrI CTTAAG 1 cut(s) 686
BisI GCNGC 3 cut(s) 390, 499, 669
BlnI CCTAGG 1 cut(s) 404
BlsI GCNGC 3 cut(s) 391, 500, 670
Bme1390I CCNGG 1 cut(s) 560
BmgT120I GGNCC 2 cut(s) 297, 610
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 560
BmsI GCATC 1 cut(s) 475
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 404
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 306
Bse118I RCCGGY 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 4 cut(s) 247, 325, 490, 757
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 2 cut(s) 294, 306
BseMII CTCAG 3 cut(s) 119, 307, 627
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 3 cut(s) 376, 485, 680
BshFI GGCC 2 cut(s) 298, 612
BshTI ACCGGT 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 2 cut(s) 367, 559
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 2 cut(s) 294, 306
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 2 cut(s) 298, 612
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
BspACI CCGC 1 cut(s) 377
BspANI GGCC 2 cut(s) 298, 612
BspCNI CTCAG 3 cut(s) 120, 306, 628
BspLI GGNNCC 1 cut(s) 244
BspPI GGATC 3 cut(s) 395, 459, 514
BspTI CTTAAG 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 366
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
BssT1I CCWWGG 1 cut(s) 404
Bst4CI ACNGT 3 cut(s) 280, 701, 765
Bst6I CTCTTC 1 cut(s) 531
BstAFI CTTAAG 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 3 cut(s) 128, 293, 636
BstEII GGTNACC 1 cut(s) 362
BstENI CCTNNNNNAGG 1 cut(s) 304
BstF5I GGATG 4 cut(s) 247, 325, 490, 757
BstKTI GATC 8 cut(s) 52, 172, 178, 240, 285, 403, 454, 522
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
BstPI GGTNACC 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 558
BstV1I GCAGC 3 cut(s) 376, 485, 680
BstV2I GAAGAC 1 cut(s) 231
BsuRI GGCC 2 cut(s) 298, 612
BtsCI GGATG 4 cut(s) 247, 325, 490, 757
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 297, 610
Csp6I GTAC 1 cut(s) 370
CspAI ACCGGT 1 cut(s) 366
CviAII CATG 2 cut(s) 86, 543
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 3 cut(s) 128, 293, 636
DpnI GATC 8 cut(s) 51, 171, 177, 239, 284, 402, 453, 521
DpnII GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
DraIII CACNNNGTG 1 cut(s) 131
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 404
Eco32I GATATC 2 cut(s) 441, 662
Eco57I CTGAAG 1 cut(s) 240
Eco91I GGTNACC 1 cut(s) 362
EcoNI CCTNNNNNAGG 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 297
EcoO65I GGTNACC 1 cut(s) 362
EcoRV GATATC 2 cut(s) 441, 662
EcoT14I CCWWGG 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 89, 546
FaiI YATR 8 cut(s) 87, 348, 350, 352, 375, 474, 544, 633
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 575
FatI CATG 2 cut(s) 85, 542
FauI CCCGC 1 cut(s) 370
FbaI TGATCA 2 cut(s) 175, 282
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 3 cut(s) 390, 499, 669
FokI GGATG 4 cut(s) 234, 332, 497, 764
Fsp4HI GCNGC 3 cut(s) 390, 499, 669
FspBI CTAG 4 cut(s) 405, 432, 614, 719
GluI GCNGC 3 cut(s) 390, 499, 669
HaeIII GGCC 2 cut(s) 298, 612
HapII CCGG 2 cut(s) 367, 559
Hin1II CATG 2 cut(s) 89, 546
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 2 cut(s) 312, 459
HpaII CCGG 2 cut(s) 367, 559
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 2 cut(s) 213, 242
Hpy8I GTNNAC 1 cut(s) 580
HpyAV CCTTC 4 cut(s) 31, 84, 403, 741
HpyCH4III ACNGT 3 cut(s) 280, 701, 765
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 501, 624
HpyF3I CTNAG 3 cut(s) 128, 293, 636
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 89, 546
Ksp22I TGATCA 2 cut(s) 175, 282
Kzo9I GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 3 cut(s) 376, 485, 680
LweI GCATC 1 cut(s) 475
MaeI CTAG 4 cut(s) 405, 432, 614, 719
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 362, 756
MalI GATC 8 cut(s) 51, 171, 177, 239, 284, 402, 453, 521
MboI GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
MboII GAAGA 6 cut(s) 69, 93, 233, 236, 548, 697
MfeI CAATTG 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 6 cut(s) 94, 112, 136, 142, 502, 775
MnlI CCTC 5 cut(s) 197, 261, 442, 532, 741
MroXI GAANNNNTTC 1 cut(s) 730
MseI TTAA 2 cut(s) 641, 687
MslI CAYNNNNRTG 1 cut(s) 258
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 2 cut(s) 367, 559
MspR9I CCNGG 1 cut(s) 560
MunI CAATTG 1 cut(s) 775
NciI CCSGG 1 cut(s) 560
NdeII GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
NlaIII CATG 2 cut(s) 89, 546
NlaIV GGNNCC 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 730
PfeI GAWTC 2 cut(s) 312, 459
PinAI ACCGGT 1 cut(s) 366
PkrI GCNGC 3 cut(s) 391, 500, 670
Psp1406I AACGTT 1 cut(s) 23
PspEI GGTNACC 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 2 cut(s) 297, 610
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 258
SalI GTCGAC 1 cut(s) 578
SaqAI TTAA 2 cut(s) 641, 687
SatI GCNGC 3 cut(s) 390, 499, 669
Sau3AI GATC 8 cut(s) 49, 169, 175, 237, 282, 400, 451, 519
Sau96I GGNCC 2 cut(s) 297, 610
ScrFI CCNGG 1 cut(s) 560
SduI GDGCHC 1 cut(s) 127
SetI ASST 7 cut(s) 26, 156, 220, 294, 345, 619, 693
SfaNI GCATC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 2 cut(s) 233, 686
SmoI CTYRAG 2 cut(s) 233, 686
Sse9I AATT 6 cut(s) 94, 112, 136, 142, 502, 775
SsiI CCGC 1 cut(s) 377
SspMI CTAG 4 cut(s) 405, 432, 614, 719
StyD4I CCNGG 1 cut(s) 558
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 3 cut(s) 280, 701, 765
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 256, 579
TasI AATT 6 cut(s) 94, 112, 136, 142, 502, 775
TfiI GAWTC 2 cut(s) 312, 459
Tru1I TTAA 2 cut(s) 641, 687
Tru9I TTAA 2 cut(s) 641, 687
TscAI CASTG 1 cut(s) 132
TseI GCWGC 3 cut(s) 389, 498, 668
TspDTI ATGAA 5 cut(s) 57, 74, 336, 666, 729
TspGWI ACGGA 2 cut(s) 415, 471
TspRI CASTG 1 cut(s) 132
Vha464I CTTAAG 1 cut(s) 686
XagI CCTNNNNNAGG 1 cut(s) 304
XbaI TCTAGA 1 cut(s) 431
XmaJI CCTAGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 730
XspI CTAG 4 cut(s) 405, 432, 614, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.