FvH4_6g29470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002834
Physical Location & Seq
Forward (+)
22764200 .. 22766265
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29470.t1

Sequence Viewer

Length: 411 bp
ATGGCGGCGGCAACTGCAGCCCCCAAGCTCCTTCTGCTCCTCCGGTACTTCTCCTCTTCCGGGCCTCCGAACACCTTGATGGGCTCTTCAAAAGTTGCCGTCCACATTCCATATTCGTGCTTCTCTTCCACCAGGGCGAAAAGGTCTCTTCCCATCCGAGCTCGCGGACGAGACCAACAAGAAGAAGATGATGATTCAAACACCACCGGTTTCAACTCACCACAGAATGATTTGGAATATCTGGGGAAGGTGCTAGCCGGTTCCATTGTGGGTGGAGCTGTAATAAAGTATGGCAGCATAGTTTTTCCTGAGATAACCAGACCCAACATCATACTGGCTCTAGTTATGATATTCACTCCTGTCGTTGTAGCTACCTTACTTCTGATCAAGCAAAGTCGTGCAAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.56

Weight (kDa)

9.66

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 165
AciI CCGC 3 cut(s) 5, 8, 165
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 60
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 3 cut(s) 90, 198, 214
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 4 cut(s) 28, 161, 278, 371
AluI AGCT 4 cut(s) 28, 161, 278, 371
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 150, 165
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 17, 294
AsiGI ACCGGT 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 210
AsuNHI GCTAGC 1 cut(s) 253
BanII GRGCYC 2 cut(s) 86, 163
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 29, 306
BccI CCATC 2 cut(s) 73, 161
BceAI ACGGC 1 cut(s) 83
BciT130I CCWGG 1 cut(s) 133
BclI TGATCA 1 cut(s) 384
BcnI CCSGG 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 150, 165
BfaI CTAG 2 cut(s) 254, 341
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 4 cut(s) 6, 9, 18, 295
BlsI GCNGC 4 cut(s) 7, 10, 19, 296
Bme1390I CCNGG 2 cut(s) 61, 133
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 2 cut(s) 61, 133
BmtI GCTAGC 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 61
BsaI GGTCTC 2 cut(s) 150, 165
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 2 cut(s) 42, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse118I RCCGGY 2 cut(s) 206, 257
Bse1I ACTGG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 1 cut(s) 339
BseRI GAGGAG 2 cut(s) 29, 43
BseXI GCAGC 2 cut(s) 29, 306
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 64
BshTI ACCGGT 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 4 cut(s) 43, 60, 207, 258
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 2 cut(s) 150, 165
BsnI GGCC 1 cut(s) 64
Bso31I GGTCTC 2 cut(s) 150, 165
Bsp1286I GDGCHC 2 cut(s) 86, 163
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 3 cut(s) 5, 8, 165
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 262
BspMAI CTGCAG 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 91
BspTNI GGTCTC 2 cut(s) 150, 165
BsrFI RCCGGY 2 cut(s) 206, 257
BsrI ACTGG 1 cut(s) 339
BssAI RCCGGY 2 cut(s) 206, 257
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 133
Bst6I CTCTTC 4 cut(s) 61, 91, 130, 153
BstC8I GCNNGC 2 cut(s) 163, 255
BstDEI CTNAG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 2 cut(s) 150, 165
BstMBI GATC 1 cut(s) 384
BstMWI GCNNNNNNNGC 3 cut(s) 14, 17, 34
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 2 cut(s) 59, 131
BstSFI CTRYAG 1 cut(s) 15
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 29, 306
BsuRI GGCC 1 cut(s) 64
BtsCI GGATG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 163, 255
Cfr10I RCCGGY 2 cut(s) 206, 257
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 46
CspAI ACCGGT 1 cut(s) 206
CviJI RGCY 9 cut(s) 20, 28, 64, 84, 161, 257, 278, 338, 371
CviKI_1 RGCY 9 cut(s) 20, 28, 64, 84, 161, 257, 278, 338, 371
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 1 cut(s) 309
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
Eam1104I CTCTTC 4 cut(s) 61, 91, 130, 153
EarI CTCTTC 4 cut(s) 61, 91, 130, 153
Ecl136II GAGCTC 1 cut(s) 161
Eco24I GRGCYC 2 cut(s) 86, 163
Eco31I GGTCTC 2 cut(s) 150, 165
Eco53kI GAGCTC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 131
EcoT38I GRGCYC 2 cut(s) 86, 163
FaiI YATR 5 cut(s) 112, 291, 299, 332, 347
FbaI TGATCA 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 6, 9, 18, 295
FokI GGATG 1 cut(s) 140
FriOI GRGCYC 2 cut(s) 86, 163
Fsp4HI GCNGC 4 cut(s) 6, 9, 18, 295
FspBI CTAG 2 cut(s) 254, 341
GluI GCNGC 4 cut(s) 6, 9, 18, 295
HaeIII GGCC 1 cut(s) 64
HapII CCGG 4 cut(s) 43, 60, 207, 258
HinfI GANTC 1 cut(s) 194
HpaII CCGG 4 cut(s) 43, 60, 207, 258
HphI GGTGA 1 cut(s) 210
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 69, 158, 384
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 41, 241
HpyCH4V TGCA 2 cut(s) 17, 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 17, 34
HpyF3I CTNAG 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 1 cut(s) 384
LguI GCTCTTC 1 cut(s) 91
LmnI GCTCC 3 cut(s) 33, 42, 275
Lsp1109I GCAGC 2 cut(s) 29, 306
MaeI CTAG 2 cut(s) 254, 341
MalI GATC 1 cut(s) 386
MboI GATC 1 cut(s) 384
MboII GAAGA 6 cut(s) 48, 78, 117, 140, 194, 197
MhlI GDGCHC 2 cut(s) 86, 163
MnlI CCTC 3 cut(s) 50, 64, 75
MslI CAYNNNNRTG 2 cut(s) 77, 115
MspI CCGG 4 cut(s) 43, 60, 207, 258
MspR9I CCNGG 2 cut(s) 61, 133
MvaI CCWGG 1 cut(s) 133
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 3 cut(s) 14, 17, 34
NciI CCSGG 1 cut(s) 61
NdeII GATC 1 cut(s) 384
NheI GCTAGC 1 cut(s) 253
NlaIV GGNNCC 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 91
PfeI GAWTC 1 cut(s) 194
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 4 cut(s) 7, 10, 19, 296
Psp124BI GAGCTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 1 cut(s) 62
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 77, 115
SacI GAGCTC 1 cut(s) 163
SapI GCTCTTC 1 cut(s) 91
SatI GCNGC 4 cut(s) 6, 9, 18, 295
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 61, 133
SduI GDGCHC 2 cut(s) 86, 163
SetI ASST 8 cut(s) 30, 77, 146, 163, 252, 280, 373, 377
SfcI CTRYAG 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 77, 115
SsiI CCGC 3 cut(s) 5, 8, 165
SspMI CTAG 2 cut(s) 254, 341
SstI GAGCTC 1 cut(s) 163
StyD4I CCNGG 2 cut(s) 59, 131
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 1 cut(s) 194
TseI GCWGC 2 cut(s) 17, 294
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XspI CTAG 2 cut(s) 254, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.