Rh2BG377400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002834
Physical Location & Seq
Reverse (-)
53329786 .. 53331622
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG377400.1

Sequence Viewer

Length: 429 bp
ATGGCGGCGGCAACTGCAGCAGCAGCTTCAGGAGCCCTCCTCCTCCGGTACTCTCCCTCTTCTGGGCCTGCAAACAACTTCATGGCCTCTTCAAAAGCTGCCATCCACATCCCACATTGCTTCTCTTCCATGGCGGCCAAAGGGTCTTTTCCCATCCGAGCTCTTGACCCTCGCAGACGACAAGAAGAAGAAGCAGAAGAGAATTCAGGCATCGTTAACAACGCCACCGGTTTCAACTCTCGGAAGGATTTGGAATATCTGGGGAAGGTGCTAGCTGGTTCCATTGTGGGTGGAGCTGTAATAAAGTATGGCAGCATAGTTTTTCCTGAGATAGCCAGACCCAACGTCATACTGGCTCTTATTATGATATTCACTCCTGTCATTCTAGCTACTTTACTTCTGATCAAGCAAAGTAGTGCACATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

14.87

Weight (kDa)

9.51

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 8, 134
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 62, 63
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 2 cut(s) 93, 235
AhdI GACNNNNNGTC 1 cut(s) 344
AluBI AGCT 6 cut(s) 26, 98, 161, 275, 296, 389
AluI AGCT 6 cut(s) 26, 98, 161, 275, 296, 389
Alw21I GWGCWC 2 cut(s) 163, 421
Alw44I GTGCAC 1 cut(s) 417
AoxI GGCC 3 cut(s) 65, 84, 135
ApaLI GTGCAC 1 cut(s) 417
ApeKI GCWGC 5 cut(s) 17, 20, 23, 98, 312
ApoI RAATTY 1 cut(s) 202
AsiGI ACCGGT 1 cut(s) 227
AspS9I GGNCC 1 cut(s) 65
AsuNHI GCTAGC 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 421
BanII GRGCYC 2 cut(s) 37, 163
Bbv12I GWGCWC 2 cut(s) 163, 421
BbvI GCAGC 5 cut(s) 29, 32, 35, 85, 324
BccI CCATC 2 cut(s) 110, 161
BclI TGATCA 1 cut(s) 402
BfaI CTAG 2 cut(s) 272, 386
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 8 cut(s) 6, 9, 18, 21, 24, 99, 135, 313
BlsI GCNGC 8 cut(s) 7, 10, 19, 22, 25, 100, 136, 314
BmeRI GACNNNNNGTC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 2 cut(s) 34, 280
BmsI GCATC 1 cut(s) 219
BmtI GCTAGC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 2 cut(s) 45, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 63
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 3 cut(s) 102, 108, 153
BseLI CCNNNNNNNGG 2 cut(s) 62, 63
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 357
BseRI GAGGAG 2 cut(s) 29, 32
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 5 cut(s) 29, 32, 35, 85, 324
BshFI GGCC 3 cut(s) 67, 86, 137
BshTI ACCGGT 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 163, 421
BsiSI CCGG 2 cut(s) 46, 228
BslI CCNNNNNNNGG 2 cut(s) 62, 63
BsnI GGCC 3 cut(s) 67, 86, 137
Bsp1286I GDGCHC 3 cut(s) 37, 163, 421
Bsp143I GATC 1 cut(s) 402
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 3 cut(s) 5, 8, 134
BspANI GGCC 3 cut(s) 67, 86, 137
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 34, 280
BspMAI CTGCAG 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 357
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 129
Bst6I CTCTTC 4 cut(s) 64, 94, 130, 192
BstC8I GCNNGC 2 cut(s) 69, 273
BstDEI CTNAG 1 cut(s) 327
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 102, 108, 153
BstKTI GATC 1 cut(s) 405
BstMBI GATC 1 cut(s) 402
BstMWI GCNNNNNNNGC 4 cut(s) 14, 17, 23, 32
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 5 cut(s) 29, 32, 35, 85, 324
BsuRI GGCC 3 cut(s) 67, 86, 137
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 3 cut(s) 102, 108, 153
Cac8I GCNNGC 2 cut(s) 69, 273
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 49
CspAI ACCGGT 1 cut(s) 227
CviAII CATG 3 cut(s) 82, 130, 422
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 1 cut(s) 327
DpnI GATC 1 cut(s) 404
DpnII GATC 1 cut(s) 402
DriI GACNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 4 cut(s) 64, 94, 130, 192
Eam1105I GACNNNNNGTC 1 cut(s) 344
EarI CTCTTC 4 cut(s) 64, 94, 130, 192
Ecl136II GAGCTC 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 37, 163
Eco53kI GAGCTC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 161
EcoRI GAATTC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 37, 163
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 3 cut(s) 85, 133, 425
FaiI YATR 7 cut(s) 83, 131, 309, 317, 350, 365, 423
FatI CATG 3 cut(s) 81, 129, 421
FbaI TGATCA 1 cut(s) 402
Fnu4HI GCNGC 8 cut(s) 6, 9, 18, 21, 24, 99, 135, 313
FokI GGATG 3 cut(s) 89, 95, 140
FriOI GRGCYC 2 cut(s) 37, 163
Fsp4HI GCNGC 8 cut(s) 6, 9, 18, 21, 24, 99, 135, 313
FspBI CTAG 2 cut(s) 272, 386
GluI GCNGC 8 cut(s) 6, 9, 18, 21, 24, 99, 135, 313
HaeIII GGCC 3 cut(s) 67, 86, 137
HapII CCGG 2 cut(s) 46, 228
Hin1II CATG 3 cut(s) 85, 133, 425
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 2 cut(s) 46, 228
Hpy166II GTNNAC 2 cut(s) 217, 419
Hpy188I TCNGA 3 cut(s) 158, 243, 402
Hpy188III TCNNGA 3 cut(s) 30, 164, 326
Hpy8I GTNNAC 2 cut(s) 217, 419
HpyAV CCTTC 2 cut(s) 238, 259
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 17, 71, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 17, 23, 32
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 3 cut(s) 85, 133, 425
Ksp22I TGATCA 1 cut(s) 402
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 1 cut(s) 402
LmnI GCTCC 2 cut(s) 32, 293
Lsp1109I GCAGC 5 cut(s) 29, 32, 35, 85, 324
LweI GCATC 1 cut(s) 219
MaeI CTAG 2 cut(s) 272, 386
MaeII ACGT 1 cut(s) 345
MalI GATC 1 cut(s) 404
MboI GATC 1 cut(s) 402
MboII GAAGA 6 cut(s) 51, 81, 117, 197, 200, 209
MhlI GDGCHC 3 cut(s) 37, 163, 421
MluCI AATT 1 cut(s) 202
MnlI CCTC 6 cut(s) 47, 50, 53, 67, 97, 180
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 424
MspI CCGG 2 cut(s) 46, 228
MwoI GCNNNNNNNGC 4 cut(s) 14, 17, 23, 32
NcoI CCATGG 1 cut(s) 129
NdeII GATC 1 cut(s) 402
NheI GCTAGC 1 cut(s) 271
NlaIII CATG 3 cut(s) 85, 133, 425
NlaIV GGNNCC 2 cut(s) 34, 280
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 8 cut(s) 7, 10, 19, 22, 25, 100, 136, 314
Psp124BI GAGCTC 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 34, 280
PspPI GGNCC 1 cut(s) 65
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 424
SacI GAGCTC 1 cut(s) 163
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 8 cut(s) 6, 9, 18, 21, 24, 99, 135, 313
Sau3AI GATC 1 cut(s) 402
Sau96I GGNCC 1 cut(s) 65
SduI GDGCHC 3 cut(s) 37, 163, 421
SetI ASST 8 cut(s) 28, 100, 163, 270, 277, 298, 348, 391
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 424
Sse9I AATT 1 cut(s) 202
SsiI CCGC 3 cut(s) 5, 8, 134
SspMI CTAG 2 cut(s) 272, 386
SstI GAGCTC 1 cut(s) 163
StyI CCWWGG 1 cut(s) 129
TaiI ACGT 1 cut(s) 348
TasI AATT 1 cut(s) 202
TauI GCSGC 3 cut(s) 8, 11, 137
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseI GCWGC 5 cut(s) 17, 20, 23, 98, 312
TspDTI ATGAA 1 cut(s) 70
VneI GTGCAC 1 cut(s) 417
XapI RAATTY 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XspI CTAG 2 cut(s) 272, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.