Prupe.3G089500_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0002834
Physical Location & Seq
Reverse (-)
6641322 .. 6644718
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G089500.1

Sequence Viewer

Length: 465 bp
ATGGAAATGGTGGCGACAACTGCCTCAGCCACACCCACAGCCCCTGGAGTCTCCAGCCTCCGCTACTCTTCCGGGTCGGGCCACCGGCGGATAAGGCCCGCAAAGAAGGTCACGGGTTACAGAAAATTCGCCATCCACTACCCAAACTGCTTCTCTTCCACGAGGGTAAAATGGTCTTTGCTTATCCGAGCTCTTGACCCTCAAAGAGGAGGAGGAGAAGAAGAAGAAGAAAGTTCAGGCATCGCTAACACTACAAGCGCTCCAAACTCTCAGGACGATCTGGAATATCTGGGGAAGGTTGTAGCTGGTTCCATTGTGGCTGCAGCTGTTATAAAGTATGGAAGCATAGTTTTCCCCGAGATAACCAGACCCAACATCGTACAGGCTCTTATCATGATATTAACTCCTGTTATTGTAGCCATTTTGCTTTTGATCAAGCAGAGCCGTGCAGAGGGGCGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.53

Weight (kDa)

9.72

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AciI CCGC 3 cut(s) 61, 88, 99
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 1 cut(s) 381
AfeI AGCGCT 1 cut(s) 259
AfiI CCNNNNNNNGG 2 cut(s) 206, 451
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 4 cut(s) 191, 305, 326, 462
AluI AGCT 4 cut(s) 191, 305, 326, 462
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 259
AoxI GGCC 2 cut(s) 79, 95
ApeKI GCWGC 2 cut(s) 320, 323
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 2 cut(s) 79, 96
AsuC2I CCSGG 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 356
BanII GRGCYC 1 cut(s) 193
BauI CACGAG 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 193
BbvCI CCTCAGC 1 cut(s) 25
BbvI GCAGC 2 cut(s) 307, 335
BccI CCATC 1 cut(s) 140
BceAI ACGGC 1 cut(s) 429
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 463
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 261
BisI GCNGC 2 cut(s) 321, 324
BlsI GCNGC 2 cut(s) 322, 325
Bme1390I CCNGG 2 cut(s) 45, 73
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 2 cut(s) 79, 96
BmiI GGNNCC 1 cut(s) 310
BmrFI CCNGG 2 cut(s) 45, 73
BmsI GCATC 1 cut(s) 249
BpmI CTGGAG 2 cut(s) 37, 66
Bpu10I CCTNAGC 1 cut(s) 25
BpuMI CCSGG 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 451
Bse118I RCCGGY 1 cut(s) 84
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 206, 451
BseMII CTCAG 2 cut(s) 39, 284
BseRI GAGGAG 3 cut(s) 222, 225, 228
BseXI GCAGC 2 cut(s) 307, 335
BshFI GGCC 2 cut(s) 81, 97
BsiHKAI GWGCWC 1 cut(s) 193
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 2 cut(s) 72, 85
BslI CCNNNNNNNGG 2 cut(s) 206, 451
BsmAI GTCTC 1 cut(s) 55
BsnI GGCC 2 cut(s) 81, 97
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 2 cut(s) 277, 432
BspACI CCGC 3 cut(s) 61, 88, 99
BspANI GGCC 2 cut(s) 81, 97
BspCNI CTCAG 2 cut(s) 38, 283
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 1 cut(s) 310
BspMAI CTGCAG 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 2 cut(s) 277, 432
BssSI CACGAG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 45
Bst6I CTCTTC 2 cut(s) 73, 160
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 25, 270
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 2 cut(s) 280, 435
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 2 cut(s) 277, 432
BstMWI GCNNNNNNNGC 2 cut(s) 20, 94
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 2 cut(s) 43, 71
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 307, 335
BsuRI GGCC 2 cut(s) 81, 97
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 99
CaiI CAGNNNCTG 1 cut(s) 44
CciI TCATGA 1 cut(s) 393
CfoI GCGC 1 cut(s) 260
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 79, 96
Csp6I GTAC 1 cut(s) 380
CviAII CATG 1 cut(s) 394
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 2 cut(s) 25, 270
DpnI GATC 2 cut(s) 279, 434
DpnII GATC 2 cut(s) 277, 432
Eam1104I CTCTTC 2 cut(s) 73, 160
EarI CTCTTC 2 cut(s) 73, 160
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 193
Eco47III AGCGCT 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 1 cut(s) 397
FaiI YATR 4 cut(s) 332, 339, 347, 395
FatI CATG 1 cut(s) 393
FauI CCCGC 1 cut(s) 106
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 321, 324
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 2 cut(s) 321, 324
FspBI CTAG 1 cut(s) 463
GlaI GCGC 1 cut(s) 259
GluI GCNGC 2 cut(s) 321, 324
GsuI CTGGAG 2 cut(s) 37, 66
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 2 cut(s) 81, 97
HapII CCGG 2 cut(s) 72, 85
HhaI GCGC 1 cut(s) 260
Hin1II CATG 1 cut(s) 397
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HinfI GANTC 1 cut(s) 48
HpaII CCGG 2 cut(s) 72, 85
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 4 cut(s) 194, 272, 281, 394
HpyAV CCTTC 2 cut(s) 100, 289
HpyCH4V TGCA 2 cut(s) 323, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 94
HpyF3I CTNAG 2 cut(s) 25, 270
Hsp92II CATG 1 cut(s) 397
HspAI GCGC 1 cut(s) 258
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 2 cut(s) 277, 432
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 2 cut(s) 307, 335
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 463
MaeIII GTNAC 2 cut(s) 109, 116
MalI GATC 2 cut(s) 279, 434
MboI GATC 2 cut(s) 277, 432
MboII GAAGA 6 cut(s) 60, 147, 230, 233, 236, 239
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 1 cut(s) 125
MlyI GAGTC 1 cut(s) 57
MnlI CCTC 8 cut(s) 34, 68, 156, 200, 203, 206, 210, 445
MseI TTAA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 2 cut(s) 72, 85
MspR9I CCNGG 2 cut(s) 45, 73
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 20, 94
NciI CCSGG 1 cut(s) 73
NdeII GATC 2 cut(s) 277, 432
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 109
PagI TCATGA 1 cut(s) 393
PkrI GCNGC 2 cut(s) 322, 325
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PsiI TTATAA 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 2 cut(s) 79, 96
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
SacI GAGCTC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 2 cut(s) 321, 324
Sau3AI GATC 2 cut(s) 277, 432
Sau96I GGNCC 2 cut(s) 79, 96
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 2 cut(s) 45, 73
SduI GDGCHC 1 cut(s) 193
SetI ASST 6 cut(s) 111, 193, 300, 307, 328, 464
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 321
SgrAI CRCCGGYG 1 cut(s) 84
Sse9I AATT 1 cut(s) 125
SsiI CCGC 3 cut(s) 61, 88, 99
SspMI CTAG 1 cut(s) 463
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 2 cut(s) 43, 71
TasI AATT 1 cut(s) 125
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 2 cut(s) 320, 323
Tsp45I GTSAC 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 204
XapI RAATTY 1 cut(s) 125
XspI CTAG 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.