RLG00000019542

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007344
Physical Location & Seq
Reverse (-)
53328493 .. 53335659
7167 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019542

Sequence Viewer

Length: 1269 bp
ATGTCTTTAACGGGTGTGACAATGTCGGAGGACGTCTGGTTAACTTGTATGACTCATGCATTGTCCACCGAGACCGAGGAGATCATGGGCCTCCTTCTTGGTGATATTGAGAACTCTGCAAATGGGGGTGTGACTGCCGTGATTTGGGGAGCGTCGCCTCAGACTCGATCAGACCGGAGGAAGGATCGTGTTGAAACTAATCCTGAGCAAATGACAATATCATCTGGAAGAACAACTAGAGTGATTGGTTGGTACCATTCACATCCTCATATTACGGTTCTTCCTTCCCATGTTGATGTACGGACTCAGGCAATGTATCAACTTCTAGATTCTGGGTTCATCGGGCTGATATTTTCCTGTTTCAGTGAAGATATAAACAAGGTTGGACGAATACAAGTCATTGCCTTTCAGTCCTCAGATGGGAAGCAGCAACACATGTCAAGACCTATATCTTTATCTCCAGTAAATAAAAGTTCAATTATAGAAGTTGACTCATCTTTAAGTACCTCAGAAAATGCACCAGTAAGATCTGGCTCGACTAGAGCAGAAGCTGCTGAACAAGACACAGCTGATTCAAGAACTTCTGGAACTATAAAGGCTGGCGGAAGATCTTCAGAACTTGGGCATTTCTTTGCTAGTGCTGAGGCAAATCATCAAGACAGAGAAAGAACCGGAGGGAACTACCCCACAGAAACTTCAGCCAACAATATTGTTGATATAGATCCCATGGATATGTCTGAGAGTATGCAAGAAGCAATGCACCGTTCGAATTTGGATATGAGTGGTGCTGAGTATGTAAGAAAAGAAGTTCCACTTTATGTTTTACCGACCTCATCTCTTATTAAGCTTGATTCACCTCTAATGTCATTTACGGAATTGCAACGAGTACTGTATGAGGAGGAGCGAGCGGCATATAACCAAGCAATCTTGCAAAATATGAGGGACGGGAAAATGCATCCACTTACTTTCATACATCACACATCAATGTATCAATCTTCCATGTGCAAATTAATCGAATATTGCTTAAGTCCTGCCATAAATGCACTGCAGTGTCGGTTAAGAGAGAATGAAATTCGGTTGGCATTGCTCACTGATGAAGTGAAGGGTTTGGAGAACGAAACACGTAGAGGAAGCGAGTCAAGTTCTGGATCTCCTCGTCAAGTTTTGTTGCCTACACTCCGCGGAAGTGCTTCTTTAGGCCATAGGGACTTGTTCAGTCCAAGGAGTGTAACAGGTCCTGGTAGTCGAAGCAGAAAAGGATCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

423

Amino Acids

46.49

Weight (kDa)

5.84

Isoelectric Point (pI)

69.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 4 - 108 6.8e-18 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Prok-JAB PF14464 10 - 103 3.2e-07 Prokaryotic homologs of the JAB domain
BRCC36_C PF18110 287 - 368 9.9e-23 BRCC36 C-terminal helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 252
AccBSI CCGCTC 1 cut(s) 908
AccII CGCG 1 cut(s) 1182
AciI CCGC 4 cut(s) 603, 908, 1180, 1182
AclWI GGATC 4 cut(s) 192, 716, 1156, 1267
AcsI RAATTY 2 cut(s) 769, 1071
AcuI CTGAAG 2 cut(s) 597, 681
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 4 cut(s) 254, 300, 505, 888
AfiI CCNNNNNNNGG 3 cut(s) 144, 181, 420
AflII CTTAAG 1 cut(s) 1024
AflIII ACRYGT 2 cut(s) 435, 1121
AgsI TTSAA 3 cut(s) 194, 477, 576
AjnI CCWGG 1 cut(s) 1237
AleI CACNNNNGTG 1 cut(s) 1048
AluBI AGCT 3 cut(s) 551, 569, 847
AluI AGCT 3 cut(s) 551, 569, 847
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 4 cut(s) 192, 716, 1156, 1267
AlwNI CAGNNNCTG 2 cut(s) 551, 1238
AoxI GGCC 2 cut(s) 88, 1198
ApeKI GCWGC 2 cut(s) 427, 551
ApoI RAATTY 2 cut(s) 769, 1071
AseI ATTAAT 1 cut(s) 1010
Asp700I GAANNNNTTC 2 cut(s) 610, 1189
Asp718I GGTACC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 88, 1235
AsuHPI GGTGA 2 cut(s) 113, 846
AsuII TTCGAA 1 cut(s) 767
AvaII GGWCC 1 cut(s) 1235
BanI GGYRCC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 439, 538
BccI CCATC 1 cut(s) 413
BceAI ACGGC 1 cut(s) 122
BcgI CGANNNNNNTGC 2 cut(s) 994, 1028
BciT130I CCWGG 1 cut(s) 1239
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 4 cut(s) 237, 326, 540, 636
BfmI CTRYAG 1 cut(s) 1046
BfrI CTTAAG 1 cut(s) 1024
BglII AGATCT 2 cut(s) 527, 608
BisI GCNGC 3 cut(s) 428, 552, 909
BlsI GCNGC 3 cut(s) 429, 553, 910
BmcAI AGTACT 1 cut(s) 888
Bme1390I CCNGG 1 cut(s) 1239
Bme18I GGWCC 1 cut(s) 1235
BmgT120I GGNCC 2 cut(s) 88, 1235
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 1239
BmsI GCATC 1 cut(s) 964
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 2 cut(s) 204, 642
Bpu14I TTCGAA 1 cut(s) 767
BsaAI YACGTR 1 cut(s) 1124
BsaBI GATNNNNATC 1 cut(s) 720
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 75, 726, 1180, 1220
BsaWI WCCGGW 2 cut(s) 174, 671
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 181, 420
Bse1I ACTGG 2 cut(s) 461, 521
Bse3DI GCAATG 4 cut(s) 318, 399, 762, 1082
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 1 cut(s) 1239
BseDI CCNNGG 4 cut(s) 75, 726, 1180, 1220
BseGI GGATG 2 cut(s) 262, 955
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 3 cut(s) 144, 181, 420
BseMI GCAATG 4 cut(s) 318, 399, 762, 1082
BseMII CTCAG 8 cut(s) 173, 195, 320, 429, 522, 633, 729, 780
BseNI ACTGG 2 cut(s) 461, 521
BseRI GAGGAG 4 cut(s) 92, 911, 914, 1143
BseXI GCAGC 2 cut(s) 439, 538
Bsh1236I CGCG 1 cut(s) 1182
BshFI GGCC 2 cut(s) 90, 1200
BshNI GGYRCC 1 cut(s) 252
BsiSI CCGG 2 cut(s) 175, 672
BslFI GGGAC 2 cut(s) 956, 1220
BslI CCNNNNNNNGG 3 cut(s) 144, 181, 420
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 956, 1220
BsnI GGCC 2 cut(s) 90, 1200
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 767
Bsp143I GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
Bsp19I CCATGG 1 cut(s) 726
BspACI CCGC 4 cut(s) 603, 908, 1180, 1182
BspANI GGCC 2 cut(s) 90, 1200
BspCNI CTCAG 8 cut(s) 172, 196, 319, 428, 521, 634, 730, 781
BspFNI CGCG 1 cut(s) 1182
BspLI GGNNCC 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 1050
BspPI GGATC 4 cut(s) 192, 716, 1156, 1267
BspT104I TTCGAA 1 cut(s) 767
BspT107I GGYRCC 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 1024
BspTNI GGTCTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 908
BsrDI GCAATG 4 cut(s) 318, 399, 762, 1082
BsrI ACTGG 2 cut(s) 461, 521
BssECI CCNNGG 4 cut(s) 75, 726, 1180, 1220
BssMI GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 726, 1220
Bst2UI CCWGG 1 cut(s) 1239
Bst4CI ACNGT 3 cut(s) 277, 764, 891
BstACI GRCGYC 1 cut(s) 33
BstAFI CTTAAG 1 cut(s) 1024
BstAPI GCANNNNNTGC 1 cut(s) 551
BstBAI YACGTR 1 cut(s) 1124
BstBI TTCGAA 1 cut(s) 767
BstC8I GCNNGC 2 cut(s) 601, 906
BstDEI CTNAG 8 cut(s) 159, 204, 306, 415, 508, 642, 738, 789
BstDSI CCRYGG 2 cut(s) 726, 1180
BstF5I GGATG 2 cut(s) 262, 955
BstFNI CGCG 1 cut(s) 1182
BstKTI GATC 8 cut(s) 84, 170, 187, 530, 611, 724, 1151, 1262
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
BstMWI GCNNNNNNNGC 2 cut(s) 551, 1040
BstNI CCWGG 1 cut(s) 1239
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 1237
BstSFI CTRYAG 1 cut(s) 1046
BstUI CGCG 1 cut(s) 1182
BstV1I GCAGC 2 cut(s) 439, 538
BstX2I RGATCY 4 cut(s) 527, 608, 721, 1148
BstYI RGATCY 4 cut(s) 527, 608, 721, 1148
BsuRI GGCC 2 cut(s) 90, 1200
BtgI CCRYGG 2 cut(s) 726, 1180
BtsCI GGATG 2 cut(s) 262, 955
BtsI GCAGTG 2 cut(s) 1043, 1055
BtsIMutI CAGTG 4 cut(s) 370, 1043, 1055, 1089
Cac8I GCNNGC 2 cut(s) 601, 906
CaiI CAGNNNCTG 2 cut(s) 551, 1238
Cfr13I GGNCC 2 cut(s) 88, 1235
Cfr42I CCGCGG 1 cut(s) 1183
CseI GACGC 1 cut(s) 141
Csp6I GTAC 4 cut(s) 253, 299, 504, 887
CviAII CATG 6 cut(s) 56, 85, 290, 436, 727, 1000
CviJI RGCY 9 cut(s) 90, 346, 534, 551, 569, 599, 701, 847, 1200
CviKI_1 RGCY 9 cut(s) 90, 346, 534, 551, 569, 599, 701, 847, 1200
CviQI GTAC 4 cut(s) 253, 299, 504, 887
DdeI CTNAG 8 cut(s) 159, 204, 306, 415, 508, 642, 738, 789
DpnI GATC 8 cut(s) 83, 169, 186, 529, 610, 723, 1150, 1261
DpnII GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
EciI GGCGGA 1 cut(s) 618
Eco130I CCWWGG 2 cut(s) 726, 1220
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 1235
Eco57I CTGAAG 2 cut(s) 597, 681
EcoO109I RGGNCCY 1 cut(s) 1235
EcoRII CCWGG 1 cut(s) 1237
EcoT14I CCWWGG 2 cut(s) 726, 1220
EcoT22I ATGCAT 2 cut(s) 61, 957
ErhI CCWWGG 2 cut(s) 726, 1220
FaeI CATG 6 cut(s) 59, 88, 293, 439, 730, 1003
FalI AAGNNNNNCTT 4 cut(s) 798, 830, 1177, 1209
FaqI GGGAC 2 cut(s) 956, 1220
FatI CATG 6 cut(s) 55, 84, 289, 435, 726, 999
Fnu4HI GCNGC 3 cut(s) 428, 552, 909
FokI GGATG 2 cut(s) 249, 942
Fsp4HI GCNGC 3 cut(s) 428, 552, 909
FspBI CTAG 4 cut(s) 237, 326, 540, 636
GluI GCNGC 3 cut(s) 428, 552, 909
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 2 cut(s) 90, 1200
HapII CCGG 2 cut(s) 175, 672
HgaI GACGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 6 cut(s) 59, 88, 293, 439, 730, 1003
HincII GTYRAC 2 cut(s) 42, 490
HindII GTYRAC 2 cut(s) 42, 490
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 8 cut(s) 52, 163, 304, 329, 491, 572, 851, 1136
HpaI GTTAAC 1 cut(s) 42
HpaII CCGG 2 cut(s) 175, 672
HphI GGTGA 2 cut(s) 113, 846
Hpy166II GTNNAC 3 cut(s) 42, 66, 490
Hpy188I TCNGA 7 cut(s) 28, 162, 172, 418, 511, 616, 739
Hpy188III TCNNGA 8 cut(s) 203, 225, 326, 441, 576, 585, 656, 1146
Hpy8I GTNNAC 3 cut(s) 42, 66, 490
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 4 cut(s) 104, 175, 294, 1096
HpyCH4III ACNGT 3 cut(s) 277, 764, 891
HpyCH4IV ACGT 2 cut(s) 33, 1123
HpyF10VI GCNNNNNNNGC 2 cut(s) 551, 1040
HpyF3I CTNAG 8 cut(s) 159, 204, 306, 415, 508, 642, 738, 789
HpySE526I ACGT 2 cut(s) 33, 1123
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 6 cut(s) 59, 88, 293, 439, 730, 1003
KpnI GGTACC 1 cut(s) 256
KspAI GTTAAC 1 cut(s) 42
KspI CCGCGG 1 cut(s) 1183
Kzo9I GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
LmnI GCTCC 2 cut(s) 149, 901
Lsp1109I GCAGC 2 cut(s) 439, 538
LweI GCATC 1 cut(s) 964
MaeI CTAG 4 cut(s) 237, 326, 540, 636
MaeII ACGT 2 cut(s) 33, 1123
MaeIII GTNAC 3 cut(s) 16, 130, 1228
MalI GATC 8 cut(s) 83, 169, 186, 529, 610, 723, 1150, 1261
MbiI CCGCTC 1 cut(s) 908
MboI GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
MboII GAAGA 6 cut(s) 240, 272, 380, 603, 618, 987
MflI RGATCY 4 cut(s) 527, 608, 721, 1148
MluCI AATT 5 cut(s) 477, 769, 875, 1007, 1071
MlyI GAGTC 5 cut(s) 46, 157, 298, 485, 1145
MmeI TCCRAC 2 cut(s) 6, 364
Mph1103I ATGCAT 2 cut(s) 61, 957
MroXI GAANNNNTTC 2 cut(s) 610, 1189
MseI TTAA 7 cut(s) 8, 41, 500, 843, 1010, 1025, 1058
MslI CAYNNNNRTG 4 cut(s) 294, 731, 983, 1048
MspA1I CMGCKG 2 cut(s) 569, 1182
MspCI CTTAAG 1 cut(s) 1024
MspI CCGG 2 cut(s) 175, 672
MspR9I CCNGG 1 cut(s) 1239
MvaI CCWGG 1 cut(s) 1239
MvnI CGCG 1 cut(s) 1182
MwoI GCNNNNNNNGC 2 cut(s) 551, 1040
NcoI CCATGG 1 cut(s) 726
NdeII GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
NlaIII CATG 6 cut(s) 59, 88, 293, 439, 730, 1003
NlaIV GGNNCC 1 cut(s) 254
NmuCI GTSAC 2 cut(s) 16, 130
NsiI ATGCAT 2 cut(s) 61, 957
NspI RCATGY 1 cut(s) 439
NspV TTCGAA 1 cut(s) 767
OliI CACNNNNGTG 1 cut(s) 1048
PciI ACATGT 1 cut(s) 435
PdmI GAANNNNTTC 2 cut(s) 610, 1189
PfeI GAWTC 3 cut(s) 329, 572, 851
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 429, 553, 910
PleI GAGTC 5 cut(s) 46, 157, 298, 485, 1144
PpsI GAGTC 5 cut(s) 46, 157, 298, 485, 1144
Ppu21I YACGTR 1 cut(s) 1124
PpuMI RGGWCCY 1 cut(s) 1235
PscI ACATGT 1 cut(s) 435
PshBI ATTAAT 1 cut(s) 1010
Psp5II RGGWCCY 1 cut(s) 1235
Psp6I CCWGG 1 cut(s) 1237
PspGI CCWGG 1 cut(s) 1237
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 2 cut(s) 88, 1235
PspPPI RGGWCCY 1 cut(s) 1235
PstI CTGCAG 1 cut(s) 1050
PstNI CAGNNNCTG 2 cut(s) 551, 1238
PsuI RGATCY 4 cut(s) 527, 608, 721, 1148
PsyI GACNNNGTC 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 4 cut(s) 254, 300, 505, 888
RsaNI GTAC 4 cut(s) 253, 299, 504, 887
RseI CAYNNNNRTG 4 cut(s) 294, 731, 983, 1048
SacII CCGCGG 1 cut(s) 1183
SaqAI TTAA 7 cut(s) 8, 41, 500, 843, 1010, 1025, 1058
SatI GCNGC 3 cut(s) 428, 552, 909
Sau3AI GATC 8 cut(s) 81, 167, 184, 527, 608, 721, 1148, 1259
Sau96I GGNCC 2 cut(s) 88, 1235
ScaI AGTACT 1 cut(s) 888
SchI GAGTC 5 cut(s) 46, 157, 298, 485, 1145
ScrFI CCNGG 1 cut(s) 1239
SfaNI GCATC 1 cut(s) 964
SfcI CTRYAG 1 cut(s) 1046
Sfr303I CCGCGG 1 cut(s) 1183
SfuI TTCGAA 1 cut(s) 767
SgrBI CCGCGG 1 cut(s) 1183
SinI GGWCC 1 cut(s) 1235
SmiMI CAYNNNNRTG 4 cut(s) 294, 731, 983, 1048
SmlI CTYRAG 1 cut(s) 1024
SmoI CTYRAG 1 cut(s) 1024
Sse9I AATT 5 cut(s) 477, 769, 875, 1007, 1071
SsiI CCGC 4 cut(s) 603, 908, 1180, 1182
SspI AATATT 2 cut(s) 709, 1019
SspMI CTAG 4 cut(s) 237, 326, 540, 636
StyD4I CCNGG 1 cut(s) 1237
StyI CCWWGG 2 cut(s) 726, 1220
TaaI ACNGT 3 cut(s) 277, 764, 891
TaiI ACGT 2 cut(s) 36, 1126
TaqI TCGA 5 cut(s) 166, 536, 767, 1014, 1246
TaqII GACCGA 1 cut(s) 89
TasI AATT 5 cut(s) 477, 769, 875, 1007, 1071
TatI WGTACW 1 cut(s) 886
TauI GCSGC 1 cut(s) 911
TfiI GAWTC 3 cut(s) 329, 572, 851
Tru1I TTAA 7 cut(s) 8, 41, 500, 843, 1010, 1025, 1058
Tru9I TTAA 7 cut(s) 8, 41, 500, 843, 1010, 1025, 1058
TscAI CASTG 4 cut(s) 370, 1050, 1055, 1096
TseFI GTSAC 2 cut(s) 16, 130
TseI GCWGC 2 cut(s) 427, 551
Tsp45I GTSAC 2 cut(s) 16, 130
TspDTI ATGAA 4 cut(s) 328, 958, 1083, 1110
TspGWI ACGGA 2 cut(s) 316, 887
TspRI CASTG 4 cut(s) 370, 1050, 1055, 1096
Tth111I GACNNNGTC 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 1024
VpaK11BI GGWCC 1 cut(s) 1235
VspI ATTAAT 1 cut(s) 1010
XapI RAATTY 2 cut(s) 769, 1071
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 1 cut(s) 439
XmnI GAANNNNTTC 2 cut(s) 610, 1189
XspI CTAG 4 cut(s) 237, 326, 540, 636
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 1 cut(s) 888
Zsp2I ATGCAT 2 cut(s) 61, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.