Prupe.3G079900_v2.0.a1

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007344
Physical Location & Seq
Forward (+)
5891815 .. 5899939
8125 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G079900.2

Sequence Viewer

Length: 1344 bp
ATGTCTTTAACGGGTGTGAAAATGTCCGAGGACGTTTGGTTGACATGTCTCACTCATGCATTGTCCACCGAGACCGAGGAGATCATGGGCCTCCTTCTTGGTGATATTGAGAACTCTGTAAATGGGGGTGTGACTGCACTAATTTGGGGAGCATCACCTCAAACGCGATCTGATCGGAGGAAGGACCGTGTGGAGACTAATCCTGAGCAGTTGGCTGCTGCATCAGCCCAGGCCGAAAGAATGACAACGTCAACAGGAAGAACAACTAGGGTGATTGGGTGGTACCATTCGCATCCTCATATTACGGTTCTTCCTTCCCATGTTGACGTGCGGACTCAGGCAATGTATCAACTTCTAGATTCTGGGTTCATTGGGCTTATATTTTCCTGTTTTAGTGAAGATATAAACAAGGTCGGACGAATCCAAGTAATTGCTTTTCAGTCCTCAGATGGGAAGCAGCATCACATGTCAAGACCTATATCTTTATCTCCTGTAAATAAAAGTCCAGTTATAGAAGTTGAATCGTCTTTAAGTACCTCAGAAAATGAACCTGCAAGATCCTTTAGAGCAGAGTGTCCTGAACAAGACACAGCTGATTCAAGAACTTCTGGAACTATAAAGGGTGGGGGACGATCTTCAGACCTGGGGCTTTTGTTTGCTAATGCTGATGCAAATCATCTAGAAAAAGAAAGAATTGGAGGAAACCACCACACTAACACTTCAGACATCATCATTGCCGATATAGATCACATGGATATGTCAGAGAGTATGCAAGAAGCAATGCACCGTTCGAATTTGGATATGAGTGGTGCCGAGTATGTCAGGAAAGAAGTTCCTCTCCATGTTTTACCGACCTCATCTCTTATTAAACTCGATTCACCATTATTATCATTTACGGAATTGCAACGAGTACTGTATGAAGAGGAGCGAGCGGCATATAACCAAGCTATCTCACAAAATATGAGGGATGGGAAAGTGCATCCACTTACTTACATACATCACACATCAATGTACCAGGCTTCCATGTGCAAATTAATTGAATATTGTTTAAGTCCTGCCATAAATGCACTGCAGTGTCGGTTAAGAGAGAATGAAATTCGGTTAGCAATGCTCACTGAAGAAGTGAAGAATTTGGAGACCGAGACACATAGAGCAAGCGAGCAAAGTTCAGGATCTCCTCGTCAAGTTTCATCGCCTCGTCAAATTTTGTCGCCTCGTCAAGTTGCGTCGCCTATACCCCGAGGAAGTACTCCGTTGGGTCACAGGGACTTGTTTAGTCCAAGTGAATCAATCAGTGCAAGAAGTCTAGCTGGTTCTGGTAGTCGAAGCCGAAAAGGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

448

Amino Acids

49.27

Weight (kDa)

6.07

Isoelectric Point (pI)

66.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 2 cut(s) 282, 809
AccBSI CCGCTC 1 cut(s) 932
AccII CGCG 1 cut(s) 166
AciI CCGC 2 cut(s) 331, 932
AclWI GGATC 2 cut(s) 552, 1180
AcsI RAATTY 4 cut(s) 793, 1095, 1129, 1203
AcuI CTGAAG 3 cut(s) 621, 705, 1137
AfaI GTAC 5 cut(s) 284, 535, 912, 1013, 1249
AfiI CCNNNNNNNGG 1 cut(s) 450
AflIII ACRYGT 2 cut(s) 44, 465
AgsI TTSAA 3 cut(s) 521, 600, 1040
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 3 cut(s) 228, 642, 1014
AleI CACNNNNGTG 1 cut(s) 1072
AluBI AGCT 3 cut(s) 593, 947, 1310
AluI AGCT 3 cut(s) 593, 947, 1310
Alw26I GTCTC 5 cut(s) 53, 65, 188, 1130, 1136
AlwI GGATC 2 cut(s) 552, 1180
Ama87I CYCGRG 1 cut(s) 1239
AoxI GGCC 2 cut(s) 88, 231
ApeKI GCWGC 3 cut(s) 215, 218, 457
ApoI RAATTY 4 cut(s) 793, 1095, 1129, 1203
AseI ATTAAT 1 cut(s) 1034
Asp718I GGTACC 1 cut(s) 282
AspS9I GGNCC 2 cut(s) 88, 184
AsuHPI GGTGA 4 cut(s) 113, 147, 283, 870
AsuII TTCGAA 1 cut(s) 791
AvaI CYCGRG 1 cut(s) 1239
AvaII GGWCC 1 cut(s) 184
BanI GGYRCC 2 cut(s) 282, 809
BbvI GCAGC 3 cut(s) 202, 205, 469
BccI CCATC 2 cut(s) 443, 962
BciT130I CCWGG 3 cut(s) 230, 644, 1016
BcoDI GTCTC 5 cut(s) 53, 65, 188, 1130, 1136
BfaI CTAG 4 cut(s) 267, 356, 680, 1307
BfmI CTRYAG 1 cut(s) 1070
BfuAI ACCTGC 1 cut(s) 559
BisI GCNGC 4 cut(s) 216, 219, 458, 933
BlsI GCNGC 4 cut(s) 217, 220, 459, 934
BmcAI AGTACT 2 cut(s) 912, 1249
Bme1390I CCNGG 3 cut(s) 230, 644, 1016
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 1239
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 2 cut(s) 88, 184
BmiI GGNNCC 2 cut(s) 284, 811
BmrFI CCNGG 3 cut(s) 230, 644, 1016
BmsI GCATC 6 cut(s) 161, 230, 301, 469, 658, 988
Bpu10I CCTNAGC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 791
BsaBI GATNNNNATC 2 cut(s) 672, 744
BsaI GGTCTC 2 cut(s) 65, 1130
BsaJI CCNNGG 5 cut(s) 27, 75, 228, 643, 1240
Bsc4I CCNNNNNNNGG 1 cut(s) 450
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 4 cut(s) 348, 732, 786, 1113
Bse8I GATNNNNATC 2 cut(s) 672, 744
BseBI CCWGG 3 cut(s) 230, 644, 1016
BseDI CCNNGG 5 cut(s) 27, 75, 228, 643, 1240
BseGI GGATG 3 cut(s) 292, 973, 979
BseJI GATNNNNATC 2 cut(s) 672, 744
BseLI CCNNNNNNNGG 1 cut(s) 450
BseMI GCAATG 4 cut(s) 348, 732, 786, 1113
BseMII CTCAG 4 cut(s) 195, 350, 459, 552
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 3 cut(s) 92, 938, 1167
BseXI GCAGC 3 cut(s) 202, 205, 469
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 2 cut(s) 90, 233
BshNI GGYRCC 2 cut(s) 282, 809
BsiHKCI CYCGRG 1 cut(s) 1239
BslFI GGGAC 2 cut(s) 642, 1280
BslI CCNNNNNNNGG 1 cut(s) 450
BsmAI GTCTC 5 cut(s) 53, 65, 188, 1130, 1136
BsmFI GGGAC 2 cut(s) 642, 1280
BsnI GGCC 2 cut(s) 90, 233
Bso31I GGTCTC 2 cut(s) 65, 1130
BsoBI CYCGRG 1 cut(s) 1239
Bsp119I TTCGAA 1 cut(s) 791
Bsp143I GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
BspACI CCGC 2 cut(s) 331, 932
BspANI GGCC 2 cut(s) 90, 233
BspCNI CTCAG 4 cut(s) 196, 349, 458, 551
BspFNI CGCG 1 cut(s) 166
BspLI GGNNCC 2 cut(s) 284, 811
BspMAI CTGCAG 1 cut(s) 1074
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 2 cut(s) 552, 1180
BspT104I TTCGAA 1 cut(s) 791
BspT107I GGYRCC 2 cut(s) 282, 809
BspTNI GGTCTC 2 cut(s) 65, 1130
BsrBI CCGCTC 1 cut(s) 932
BsrDI GCAATG 4 cut(s) 348, 732, 786, 1113
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 5 cut(s) 27, 75, 228, 643, 1240
BssMI GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
Bst2UI CCWGG 3 cut(s) 230, 644, 1016
Bst4CI ACNGT 4 cut(s) 188, 307, 788, 915
Bst6I CTCTTC 1 cut(s) 915
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 3 cut(s) 930, 1156, 1160
BstDEI CTNAG 4 cut(s) 204, 336, 445, 538
BstF5I GGATG 3 cut(s) 292, 973, 979
BstFNI CGCG 1 cut(s) 166
BstKTI GATC 8 cut(s) 84, 170, 175, 560, 635, 748, 1175, 1340
BstMAI GTCTC 5 cut(s) 53, 65, 188, 1130, 1136
BstMBI GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
BstMWI GCNNNNNNNGC 2 cut(s) 224, 1064
BstNI CCWGG 3 cut(s) 230, 644, 1016
BstNSI RCATGY 2 cut(s) 48, 469
BstSCI CCNGG 3 cut(s) 228, 642, 1014
BstSFI CTRYAG 1 cut(s) 1070
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 3 cut(s) 202, 205, 469
BstX2I RGATCY 2 cut(s) 557, 1172
BstYI RGATCY 2 cut(s) 557, 1172
BsuRI GGCC 2 cut(s) 90, 233
BtgZI GCGATG 1 cut(s) 1176
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 3 cut(s) 292, 973, 979
BtsI GCAGTG 2 cut(s) 1067, 1079
BtsIMutI CAGTG 4 cut(s) 1067, 1079, 1113, 1300
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 3 cut(s) 930, 1156, 1160
Cfr13I GGNCC 2 cut(s) 88, 184
CseI GACGC 1 cut(s) 1215
Csp6I GTAC 5 cut(s) 283, 534, 911, 1012, 1248
CviAII CATG 8 cut(s) 45, 56, 85, 320, 466, 751, 842, 1024
CviQI GTAC 5 cut(s) 283, 534, 911, 1012, 1248
DdeI CTNAG 4 cut(s) 204, 336, 445, 538
DpnI GATC 8 cut(s) 83, 169, 174, 559, 634, 747, 1174, 1339
DpnII GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Eco31I GGTCTC 2 cut(s) 65, 1130
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 3 cut(s) 621, 705, 1137
Eco88I CYCGRG 1 cut(s) 1239
EcoRII CCWGG 3 cut(s) 228, 642, 1014
EcoT22I ATGCAT 1 cut(s) 61
FaeI CATG 8 cut(s) 48, 59, 88, 323, 469, 754, 845, 1027
FaqI GGGAC 2 cut(s) 642, 1280
FatI CATG 8 cut(s) 44, 55, 84, 319, 465, 750, 841, 1023
Fnu4HI GCNGC 4 cut(s) 216, 219, 458, 933
FokI GGATG 3 cut(s) 279, 966, 980
Fsp4HI GCNGC 4 cut(s) 216, 219, 458, 933
FspBI CTAG 4 cut(s) 267, 356, 680, 1307
GluI GCNGC 4 cut(s) 216, 219, 458, 933
HaeIII GGCC 2 cut(s) 90, 233
HgaI GACGC 1 cut(s) 1215
Hin1II CATG 8 cut(s) 48, 59, 88, 323, 469, 754, 845, 1027
HincII GTYRAC 3 cut(s) 42, 252, 325
HindII GTYRAC 3 cut(s) 42, 252, 325
HinfI GANTC 7 cut(s) 334, 359, 420, 521, 596, 875, 1286
HphI GGTGA 4 cut(s) 113, 147, 283, 870
Hpy166II GTNNAC 4 cut(s) 42, 66, 252, 325
Hpy188I TCNGA 9 cut(s) 28, 172, 177, 416, 448, 541, 640, 724, 763
Hpy188III TCNNGA 9 cut(s) 203, 356, 471, 578, 600, 609, 680, 823, 1170
Hpy8I GTNNAC 4 cut(s) 42, 66, 252, 325
Hpy99I CGWCG 1 cut(s) 1231
HpyAV CCTTC 3 cut(s) 104, 175, 324
HpyCH4III ACNGT 4 cut(s) 188, 307, 788, 915
HpyCH4IV ACGT 3 cut(s) 33, 248, 327
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 1064
HpyF3I CTNAG 4 cut(s) 204, 336, 445, 538
HpySE526I ACGT 3 cut(s) 33, 248, 327
Hsp92II CATG 8 cut(s) 48, 59, 88, 323, 469, 754, 845, 1027
KpnI GGTACC 1 cut(s) 286
Kzo9I GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
LmnI GCTCC 2 cut(s) 149, 925
Lsp1109I GCAGC 3 cut(s) 202, 205, 469
LweI GCATC 6 cut(s) 161, 230, 301, 469, 658, 988
MaeI CTAG 4 cut(s) 267, 356, 680, 1307
MaeII ACGT 3 cut(s) 33, 248, 327
MaeIII GTNAC 2 cut(s) 130, 1259
MalI GATC 8 cut(s) 83, 169, 174, 559, 634, 747, 1174, 1339
MbiI CCGCTC 1 cut(s) 932
MboI GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
MboII GAAGA 7 cut(s) 270, 302, 410, 627, 932, 1130, 1138
MflI RGATCY 2 cut(s) 557, 1172
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 394
Mph1103I ATGCAT 1 cut(s) 61
MseI TTAA 6 cut(s) 8, 530, 867, 1034, 1049, 1082
MslI CAYNNNNRTG 3 cut(s) 755, 1007, 1072
MspA1I CMGCKG 1 cut(s) 593
MspR9I CCNGG 3 cut(s) 230, 644, 1016
MvaI CCWGG 3 cut(s) 230, 644, 1016
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 2 cut(s) 224, 1064
NdeII GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
NlaIII CATG 8 cut(s) 48, 59, 88, 323, 469, 754, 845, 1027
NlaIV GGNNCC 2 cut(s) 284, 811
NmeAIII GCCGAG 1 cut(s) 838
NmuCI GTSAC 2 cut(s) 130, 1259
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 2 cut(s) 48, 469
NspV TTCGAA 1 cut(s) 791
OliI CACNNNNGTG 1 cut(s) 1072
PciI ACATGT 2 cut(s) 44, 465
PfeI GAWTC 6 cut(s) 359, 420, 521, 596, 875, 1286
PflFI GACNNNGTC 1 cut(s) 247
PkrI GCNGC 4 cut(s) 217, 220, 459, 934
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PscI ACATGT 2 cut(s) 44, 465
PshBI ATTAAT 1 cut(s) 1034
Psp6I CCWGG 3 cut(s) 228, 642, 1014
PspGI CCWGG 3 cut(s) 228, 642, 1014
PspN4I GGNNCC 2 cut(s) 284, 811
PspPI GGNCC 2 cut(s) 88, 184
PstI CTGCAG 1 cut(s) 1074
PsuI RGATCY 2 cut(s) 557, 1172
PsyI GACNNNGTC 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 5 cut(s) 284, 535, 912, 1013, 1249
RsaNI GTAC 5 cut(s) 283, 534, 911, 1012, 1248
RseI CAYNNNNRTG 3 cut(s) 755, 1007, 1072
SaqAI TTAA 6 cut(s) 8, 530, 867, 1034, 1049, 1082
SatI GCNGC 4 cut(s) 216, 219, 458, 933
Sau3AI GATC 8 cut(s) 81, 167, 172, 557, 632, 745, 1172, 1337
Sau96I GGNCC 2 cut(s) 88, 184
ScaI AGTACT 2 cut(s) 912, 1249
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 230, 644, 1016
SfaNI GCATC 6 cut(s) 161, 230, 301, 469, 658, 988
SfcI CTRYAG 1 cut(s) 1070
SfuI TTCGAA 1 cut(s) 791
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 3 cut(s) 755, 1007, 1072
SsiI CCGC 2 cut(s) 331, 932
SspI AATATT 1 cut(s) 1043
SspMI CTAG 4 cut(s) 267, 356, 680, 1307
StyD4I CCNGG 3 cut(s) 228, 642, 1014
TaaI ACNGT 4 cut(s) 188, 307, 788, 915
TaiI ACGT 3 cut(s) 36, 251, 330
TaqI TCGA 3 cut(s) 791, 873, 1324
TaqII GACCGA 2 cut(s) 89, 1154
TatI WGTACW 2 cut(s) 910, 1247
TauI GCSGC 1 cut(s) 935
TfiI GAWTC 6 cut(s) 359, 420, 521, 596, 875, 1286
Tru1I TTAA 6 cut(s) 8, 530, 867, 1034, 1049, 1082
Tru9I TTAA 6 cut(s) 8, 530, 867, 1034, 1049, 1082
TscAI CASTG 4 cut(s) 1074, 1079, 1120, 1300
TseFI GTSAC 2 cut(s) 130, 1259
TseI GCWGC 3 cut(s) 215, 218, 457
Tsp45I GTSAC 2 cut(s) 130, 1259
TspDTI ATGAA 5 cut(s) 358, 561, 933, 1107, 1179
TspGWI ACGGA 2 cut(s) 911, 1242
TspRI CASTG 4 cut(s) 1074, 1079, 1120, 1300
Tth111I GACNNNGTC 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 1034
XapI RAATTY 4 cut(s) 793, 1095, 1129, 1203
XbaI TCTAGA 2 cut(s) 355, 679
XceI RCATGY 2 cut(s) 48, 469
XspI CTAG 4 cut(s) 267, 356, 680, 1307
ZrmI AGTACT 2 cut(s) 912, 1249
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.