Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008226

Physical Location & Seq

Genomic Coordinates Forward (+)

31816906 .. 31818901

Gene Structure 1996 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1248700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGCTACAGAGCAGCTTTTTCCAGTGGTTGTGGCTCAAGATAACTCCATTGGACAAGATAAATGTTCCTTTGAAAAATGGAATACCGTATCTGATGGAGTCGCGGACTCCGACAACAAATCCTGTGGCACCTTCGACTGCAGCATCTGCTTAGACTCTGTGCAGGATCCTGTGGTCACCCTCTGCGGTCACCTCTACTGCTGGCCTTGCATATACAAGTGGCTTCAGTTCCAAAGCCCGTCTTCCGAAAACCAAGATGAGCAGAAACAGCCCCAGTGCCCTGTATGCAAAGCTGAAGTCTCCGAGTCCTCTCTAGTCCCACTCTACGGCCGGGGACAAACAACCAAACCCCAAAAGCCCTCGAAAGGCAAGGCTCCTCATCTTGGTATAGTGATTCCGCGAAGACCCTTTCGTCCTTGTGGGGCTGACACACCGCGATCAGCCCGTACACCCACCAGTCAATACGTTACTCCGCAACTTCATCATCGGAGTTCTCCTTACGGTTCACAACTAAACAGTCCCCTACAAGACCCAATGAATAGTCCTGGCGTCGCCACAACCGCGATCAATCCAGGAGCTGACATGTTTGGTGAAATGGTATATGCAAGGGTTTTTGGCAACTCGTTAACAAACTTGTATACCAATTCGAATTCGTATAATCTGGCGGGGAGCGGTAGTCCGAGGGTTAGAAGGCATGTAATGCAGGCTGATAAGTCGCTCAGCAGAATATGTTTTTTTCTCTTCTGTTGCTTTGTGCTGTGCCTTGTTTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009611 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009987 GO:0010033 GO:0010035 GO:0010243 GO:0010498 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0031984 GO:0032446 GO:0032527 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0051788 GO:0070647 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071702 GO:0071704 GO:0071705 GO:0071712 GO:0098796 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

259

Amino Acids

28.42

Weight (kDa)

8.17

Isoelectric Point (pI)

52.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	46 - 97	2e-07	Ring finger domain
zf-C3HC4_3	PF13920	46 - 102	4.6e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	47 - 96	4.9e-10	Zinc finger, C3HC4 type (RING finger)
RING_XB3-XBAT31	PF24921	47 - 100	2.8e-10	E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-RING_UBOX	PF13445	47 - 94	7.3e-10	RING-type zinc-finger
zf-C3HC4_2	PF13923	47 - 76	1.8e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	47 - 97	5.6e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03510 AT4G03510 AT4G03510 AT4G03510
fragaria_vesca	FvH4_6g26170
malus_domestica	MD09G1248700.v1.1 MD17G1241200.v1.1
prunus_persica	Prupe.3G137000_v2.0.a1
pyrus_communis	pycom09g16560 pycom17g24430 pycom17g24440 pycom17g24450
rosa_chinensis	RchiOBHm_Chr2g0126321
rosa_laevigata	RLG00000018901
rosa_multiflora	Rmu_ssc0000264.1_g000040
rosa_roxburghii	Rroxscaffold_2G00117240
rosa_rugosa	Rorug02G0262600 Rorug02G0262700
rosa_samantha	Rh2AG319500 Rh2BG327900 Rh2CG307800 Rh2DG347600
rosa_wichuraiana	Rw2G025890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	411
AccB1I	GGYRCC	1 cut(s)	128
AccBSI	CCGCTC	1 cut(s)	672
AccI	GTMKAC	1 cut(s)	638
AccII	CGCG	4 cut(s)	104, 400, 436, 563
AciI	CCGC	8 cut(s)	104, 186, 398, 434, 473, 561, 665, 672
AclWI	GGATC	2 cut(s)	161, 174
AcoI	YGGCCR	1 cut(s)	328
AcsI	RAATTY	1 cut(s)	649
AcuI	CTGAAG	2 cut(s)	209, 315
AcyI	GRCGYC	1 cut(s)	549
AfaI	GTAC	1 cut(s)	448
AfiI	CCNNNNNNNGG	3 cut(s)	326, 365, 383
AflIII	ACRYGT	1 cut(s)	582
AgsI	TTSAA	1 cut(s)	74
AjnI	CCWGG	2 cut(s)	544, 571
AluBI	AGCT	3 cut(s)	16, 293, 578
AluI	AGCT	3 cut(s)	16, 293, 578
Alw26I	GTCTC	1 cut(s)	304
AlwI	GGATC	2 cut(s)	161, 174
AlwNI	CAGNNNCTG	2 cut(s)	147, 578
AoxI	GGCC	2 cut(s)	203, 328
ApeKI	GCWGC	2 cut(s)	13, 141
ApoI	RAATTY	1 cut(s)	649
ArsI	GACNNNNNNTTYG	2 cut(s)	282, 314
AsuC2I	CCSGG	1 cut(s)	332
AsuHPI	GGTGA	3 cut(s)	169, 182, 602
AsuII	TTCGAA	1 cut(s)	647
BaeGI	GKGCMC	1 cut(s)	281
BamHI	GGATCC	1 cut(s)	166
BanI	GGYRCC	1 cut(s)	128
BbsI	GAAGAC	2 cut(s)	234, 409
BbvI	GCAGC	2 cut(s)	25, 153
BccI	CCATC	1 cut(s)	89
BceAI	ACGGC	1 cut(s)	343
BciT130I	CCWGG	2 cut(s)	546, 573
BcnI	CCSGG	1 cut(s)	332
BcoDI	GTCTC	1 cut(s)	304
BfaI	CTAG	1 cut(s)	314
BfmI	CTRYAG	2 cut(s)	6, 139
BisI	GCNGC	2 cut(s)	14, 142
BlpI	GCTNAGC	1 cut(s)	719
BlsI	GCNGC	2 cut(s)	15, 143
Bme1390I	CCNGG	3 cut(s)	332, 546, 573
BmiI	GGNNCC	3 cut(s)	130, 168, 375
BmrFI	CCNGG	3 cut(s)	332, 546, 573
BmrI	ACTGGG	1 cut(s)	268
BmsI	GCATC	1 cut(s)	153
BmuI	ACTGGG	1 cut(s)	268
BpiI	GAAGAC	2 cut(s)	234, 409
Bpu1102I	GCTNAGC	1 cut(s)	719
Bpu14I	TTCGAA	1 cut(s)	647
BpuEI	CTTGAG	1 cut(s)	21
BpuMI	CCSGG	1 cut(s)	332
BsaHI	GRCGYC	1 cut(s)	549
BsaJI	CCNNGG	2 cut(s)	331, 680
BsaXI	ACNNNNNCTCC	2 cut(s)	661, 691
Bsc4I	CCNNNNNNNGG	3 cut(s)	326, 365, 383
Bse1I	ACTGG	3 cut(s)	23, 274, 456
BseBI	CCWGG	2 cut(s)	546, 573
BseDI	CCNNGG	2 cut(s)	331, 680
BseLI	CCNNNNNNNGG	3 cut(s)	326, 365, 383
BseMII	CTCAG	1 cut(s)	733
BseNI	ACTGG	3 cut(s)	23, 274, 456
BseRI	GAGGAG	1 cut(s)	366
BseSI	GKGCMC	1 cut(s)	281
BseX3I	CGGCCG	1 cut(s)	328
BseXI	GCAGC	2 cut(s)	25, 153
BsgI	GTGCAG	1 cut(s)	182
Bsh1236I	CGCG	4 cut(s)	104, 400, 436, 563
Bsh1285I	CGRYCG	1 cut(s)	331
BshFI	GGCC	2 cut(s)	205, 330
BshNI	GGYRCC	1 cut(s)	128
BsiEI	CGRYCG	1 cut(s)	331
BsiSI	CCGG	1 cut(s)	331
BslFI	GGGAC	3 cut(s)	302, 348, 504
BslI	CCNNNNNNNGG	3 cut(s)	326, 365, 383
BsmAI	GTCTC	1 cut(s)	304
BsmFI	GGGAC	3 cut(s)	302, 348, 504
BsnI	GGCC	2 cut(s)	205, 330
Bsp119I	TTCGAA	1 cut(s)	647
Bsp1286I	GDGCHC	1 cut(s)	281
Bsp143I	GATC	3 cut(s)	166, 437, 564
Bsp1720I	GCTNAGC	1 cut(s)	719
BspACI	CCGC	8 cut(s)	104, 186, 398, 434, 473, 561, 665, 672
BspANI	GGCC	2 cut(s)	205, 330
BspCNI	CTCAG	1 cut(s)	732
BspFNI	CGCG	4 cut(s)	104, 400, 436, 563
BspLI	GGNNCC	3 cut(s)	130, 168, 375
BspMAI	CTGCAG	1 cut(s)	143
BspPI	GGATC	2 cut(s)	161, 174
BspT104I	TTCGAA	1 cut(s)	647
BspT107I	GGYRCC	1 cut(s)	128
BsrBI	CCGCTC	1 cut(s)	672
BsrI	ACTGG	3 cut(s)	23, 274, 456
BssECI	CCNNGG	2 cut(s)	331, 680
BssMI	GATC	3 cut(s)	166, 437, 564
BssNAI	GTATAC	1 cut(s)	639
BssNI	GRCGYC	1 cut(s)	549
Bst1107I	GTATAC	1 cut(s)	639
Bst2UI	CCWGG	2 cut(s)	546, 573
Bst4CI	ACNGT	3 cut(s)	88, 503, 518
Bst6I	CTCTTC	1 cut(s)	746
BstACI	GRCGYC	1 cut(s)	549
BstAPI	GCANNNNNTGC	2 cut(s)	147, 700
BstBI	TTCGAA	1 cut(s)	647
BstC8I	GCNNGC	2 cut(s)	203, 705
BstDEI	CTNAG	2 cut(s)	151, 719
BstEII	GGTNACC	2 cut(s)	175, 188
BstFNI	CGCG	4 cut(s)	104, 400, 436, 563
BstKTI	GATC	3 cut(s)	169, 440, 567
BstMAI	GTCTC	1 cut(s)	304
BstMBI	GATC	3 cut(s)	166, 437, 564
BstMCI	CGRYCG	1 cut(s)	331
BstMWI	GCNNNNNNNGC	6 cut(s)	147, 207, 268, 285, 560, 700
BstNI	CCWGG	2 cut(s)	546, 573
BstNSI	RCATGY	2 cut(s)	586, 698
BstPI	GGTNACC	2 cut(s)	175, 188
BstSCI	CCNGG	3 cut(s)	330, 544, 571
BstSFI	CTRYAG	2 cut(s)	6, 139
BstSLI	GKGCMC	1 cut(s)	281
BstUI	CGCG	4 cut(s)	104, 400, 436, 563
BstV1I	GCAGC	2 cut(s)	25, 153
BstV2I	GAAGAC	2 cut(s)	234, 409
BstX2I	RGATCY	1 cut(s)	166
BstYI	RGATCY	1 cut(s)	166
BstZ17I	GTATAC	1 cut(s)	639
BstZI	CGGCCG	1 cut(s)	328
BsuRI	GGCC	2 cut(s)	205, 330
BtsIMutI	CAGTG	2 cut(s)	30, 281
Cac8I	GCNNGC	2 cut(s)	203, 705
CaiI	CAGNNNCTG	2 cut(s)	147, 578
CseI	GACGC	1 cut(s)	538
Csp6I	GTAC	1 cut(s)	447
CspCI	CAANNNNNGTGG	2 cut(s)	106, 141
CviAII	CATG	2 cut(s)	583, 695
CviQI	GTAC	1 cut(s)	447
DdeI	CTNAG	2 cut(s)	151, 719
DpnI	GATC	3 cut(s)	168, 439, 566
DpnII	GATC	3 cut(s)	166, 437, 564
DrdI	GACNNNNNNGTC	1 cut(s)	411
DseDI	GACNNNNNNGTC	1 cut(s)	411
EaeI	YGGCCR	1 cut(s)	328
EagI	CGGCCG	1 cut(s)	328
Eam1104I	CTCTTC	1 cut(s)	746
EarI	CTCTTC	1 cut(s)	746
EclXI	CGGCCG	1 cut(s)	328
Eco52I	CGGCCG	1 cut(s)	328
Eco57I	CTGAAG	2 cut(s)	209, 315
Eco91I	GGTNACC	2 cut(s)	175, 188
EcoO65I	GGTNACC	2 cut(s)	175, 188
EcoRI	GAATTC	1 cut(s)	649
EcoRII	CCWGG	2 cut(s)	544, 571
FaeI	CATG	2 cut(s)	586, 698
FalI	AAGNNNNNCTT	2 cut(s)	226, 258
FaqI	GGGAC	3 cut(s)	302, 348, 504
FatI	CATG	2 cut(s)	582, 694
FauI	CCCGC	1 cut(s)	658
FblI	GTMKAC	1 cut(s)	638
Fnu4HI	GCNGC	2 cut(s)	14, 142
Fsp4HI	GCNGC	2 cut(s)	14, 142
FspBI	CTAG	1 cut(s)	314
GluI	GCNGC	2 cut(s)	14, 142
HaeIII	GGCC	2 cut(s)	205, 330
HapII	CCGG	1 cut(s)	331
HgaI	GACGC	1 cut(s)	538
Hin1I	GRCGYC	1 cut(s)	549
Hin1II	CATG	2 cut(s)	586, 698
HincII	GTYRAC	1 cut(s)	627
HindII	GTYRAC	1 cut(s)	627
HinfI	GANTC	5 cut(s)	99, 107, 155, 305, 394
HpaI	GTTAAC	1 cut(s)	627
HpaII	CCGG	1 cut(s)	331
HphI	GGTGA	3 cut(s)	169, 182, 602
Hpy166II	GTNNAC	4 cut(s)	449, 506, 627, 639
Hpy188I	TCNGA	7 cut(s)	94, 112, 247, 304, 489, 681, 776
Hpy188III	TCNNGA	1 cut(s)	38
Hpy8I	GTNNAC	4 cut(s)	449, 506, 627, 639
Hpy99I	CGWCG	1 cut(s)	554
HpyAV	CCTTC	2 cut(s)	142, 684
HpyCH4III	ACNGT	3 cut(s)	88, 503, 518
HpyCH4IV	ACGT	1 cut(s)	465
HpyCH4V	TGCA	6 cut(s)	141, 163, 210, 288, 605, 703
HpyF10VI	GCNNNNNNNGC	6 cut(s)	147, 207, 268, 285, 560, 700
HpyF3I	CTNAG	2 cut(s)	151, 719
HpySE526I	ACGT	1 cut(s)	465
Hsp92I	GRCGYC	1 cut(s)	549
Hsp92II	CATG	2 cut(s)	586, 698
KspAI	GTTAAC	1 cut(s)	627
Kzo9I	GATC	3 cut(s)	166, 437, 564
LmnI	GCTCC	3 cut(s)	379, 575, 669
Lsp1109I	GCAGC	2 cut(s)	25, 153
LweI	GCATC	1 cut(s)	153
MaeI	CTAG	1 cut(s)	314
MaeII	ACGT	1 cut(s)	465
MaeIII	GTNAC	3 cut(s)	175, 188, 466
MalI	GATC	3 cut(s)	168, 439, 566
MbiI	CCGCTC	1 cut(s)	672
MboI	GATC	3 cut(s)	166, 437, 564
MboII	GAAGA	3 cut(s)	234, 414, 733
MflI	RGATCY	1 cut(s)	166
MhlI	GDGCHC	1 cut(s)	281
MluCI	AATT	2 cut(s)	643, 649
MlyI	GAGTC	4 cut(s)	101, 108, 149, 314
MmeI	TCCRAC	1 cut(s)	135
MnlI	CCTC	6 cut(s)	191, 203, 319, 370, 387, 675
MseI	TTAA	1 cut(s)	626
MspI	CCGG	1 cut(s)	331
MspR9I	CCNGG	3 cut(s)	332, 546, 573
MvaI	CCWGG	2 cut(s)	546, 573
MvnI	CGCG	4 cut(s)	104, 400, 436, 563
MwoI	GCNNNNNNNGC	6 cut(s)	147, 207, 268, 285, 560, 700
NciI	CCSGG	1 cut(s)	332
NdeII	GATC	3 cut(s)	166, 437, 564
NlaIII	CATG	2 cut(s)	586, 698
NlaIV	GGNNCC	3 cut(s)	130, 168, 375
NmuCI	GTSAC	2 cut(s)	175, 188
NspI	RCATGY	2 cut(s)	586, 698
NspV	TTCGAA	1 cut(s)	647
PciI	ACATGT	1 cut(s)	582
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PfeI	GAWTC	1 cut(s)	394
PfoI	TCCNGGA	1 cut(s)	571
PkrI	GCNGC	2 cut(s)	15, 143
PleI	GAGTC	4 cut(s)	101, 107, 149, 313
PpsI	GAGTC	4 cut(s)	101, 107, 149, 313
PscI	ACATGT	1 cut(s)	582
Psp6I	CCWGG	2 cut(s)	544, 571
PspEI	GGTNACC	2 cut(s)	175, 188
PspGI	CCWGG	2 cut(s)	544, 571
PspN4I	GGNNCC	3 cut(s)	130, 168, 375
PstI	CTGCAG	1 cut(s)	143
PstNI	CAGNNNCTG	2 cut(s)	147, 578
PsuI	RGATCY	1 cut(s)	166
RsaI	GTAC	1 cut(s)	448
RsaNI	GTAC	1 cut(s)	447
SaqAI	TTAA	1 cut(s)	626
SatI	GCNGC	2 cut(s)	14, 142
Sau3AI	GATC	3 cut(s)	166, 437, 564
SchI	GAGTC	4 cut(s)	101, 108, 149, 314
ScrFI	CCNGG	3 cut(s)	332, 546, 573
SduI	GDGCHC	1 cut(s)	281
SetI	ASST	6 cut(s)	18, 134, 195, 295, 468, 580
SfaNI	GCATC	1 cut(s)	153
SfcI	CTRYAG	2 cut(s)	6, 139
SfuI	TTCGAA	1 cut(s)	647
SmlI	CTYRAG	1 cut(s)	36
SmoI	CTYRAG	1 cut(s)	36
Sse9I	AATT	2 cut(s)	643, 649
SsiI	CCGC	8 cut(s)	104, 186, 398, 434, 473, 561, 665, 672
SspMI	CTAG	1 cut(s)	314
StyD4I	CCNGG	3 cut(s)	330, 544, 571
TaaI	ACNGT	3 cut(s)	88, 503, 518
TaiI	ACGT	1 cut(s)	468
TaqI	TCGA	3 cut(s)	135, 362, 647
TasI	AATT	2 cut(s)	643, 649
TfiI	GAWTC	1 cut(s)	394
Tru1I	TTAA	1 cut(s)	626
Tru9I	TTAA	1 cut(s)	626
TscAI	CASTG	2 cut(s)	30, 281
TseFI	GTSAC	2 cut(s)	175, 188
TseI	GCWGC	2 cut(s)	13, 141
Tsp45I	GTSAC	2 cut(s)	175, 188
TspDTI	ATGAA	2 cut(s)	470, 551
TspRI	CASTG	2 cut(s)	30, 281
XapI	RAATTY	1 cut(s)	649
XceI	RCATGY	2 cut(s)	586, 698
XmiI	GTMKAC	1 cut(s)	638
XspI	CTAG	1 cut(s)	314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.