Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0007603

Physical Location & Seq

Genomic Coordinates Forward (+)

35925291 .. 35936264

Gene Structure 10974 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1282200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2526 bp

ATGAAGCTTTCAACATCAGGGTTGGGTCAGCAGGATCATGAAGGAGGAGGAGGATTGGAAAAGAAGTGCTTGAATTCGGAGCTGTGGCATGCGTGCGCGGGACCTCTGGTGTCCCTGCCGACACCTGGGACTCGTGTAGTCTACTTTCCGCAGGGCCACAGCGACCAGGTTGCTGCCACTACCAACAAGCAAGTTGATGCCCACATACCAAACTACCCGAACTTGCCACCCCAGTTAATCTGTCAGCTCCACAATGTGACAATGCATGCAGATGTGGAGACAGATGAAGTATATGCCCAAATGACATTGCAGCCTTTGACGCCGCAAGAACAAAAGGAAACATTTCTTCCAATGGAGTTGGGGCTTCCAAGCAAGCAACCGACAAACTACTTTTGCAAGACATTGACGGCGAGTGATACTAGTACACACGGAGGCTTCTCTGTTCCACGTCGTGCTGCCGAGAAAGTTTTTCCTCCACTGGATTTTTCGCTACAACCTCCAGCTCAGGAACTAATTGCTAGGGATCTCCATGATGTTGAGTGGAAGTTTAGGCATATTTTTCGAGGACAGCCGAAACGACATCTTCTTACTACAGGCTGGAGTGTGTTTGTTAGTGCCAAAAGACTTGTTGCCGGAGATTCTGTTCTTTTTATTTGGAATGAAAAGAATCAGCTTCTTTTGGGGATTCGCCATGCTACTCGACCACAAACTGTGATGCCGTCTTCTGTTTTGTCAAGCGATAGCATGCATATTGGACTTCTTGCAGCTGCGGCTCATGCAGCTTCAACTAATAGCTGTTTTACGGTTTTTTATAATCCAAGGGCTAGTCCATCTGAGTTTGTCATACCCCTATCAAAATACATTAAAGCAGTATTTCACACACGTGTTTCAGTTGGGATGCGATTCCGGATGCTATTTGAGACTGAAGAGTCTAGTGTCAGAAGGTACATGGGTACGATAACTGGCATAAGTGACCTGGATCCTGTTCGGTGGCCAAATTCTCATTGGCGATCTGTCAAGGTTGGTTGGGATGAGTCAACTTCAGGTGAGAGGCAGCCAAGGGTATCGTTATGGGAGATTGAGCCTTTGACAACTTTCCCCATGTATCCATCACTGTTTCCCCTTCGACTGAAACGTCCTTGGATTCCTGGGGCCTCATCTATGCATGATAATAGAGATGAAGCTAATAGCTTAATGTGGCTAAGGGGGGGAGCTGGGGAGCAAGGTCTTCAGTCAATGAACTTTCAAAATGTAGGTATGTTTCCATGGATGCAACAGAGATTGGATTCAACATTGATTGGAAATGATCACAATCAGCAGTACCAGTCCATGTTGGCTGCTGGTTTGCAGAATCTAGGAAGTGGGGATCAACTAAGACAACAGATGATGCACTTTCAGCAGCCTTTTCAGTATGTTCAGCAGCCGGGTAGCCACAATCCGATGCTGCAGTTACAACAGCAAGCAATTCAGCAGTCAATCCCTCATAATATCCTGCAGGGACAGCCACAAGTTTCAATGGAGAATATGCATCAGCAACTTCTCCAGCAACAATTTAACAATCAAACTGATGCGCAGGTGCAGCAACAGCAAAATGCCTATCAGGATGCACTCAAGGTTCAAAATGAACAGCTCCATCAGAGTCATGTGCCATCTCCATCATTTACAAAACCAGACTTCAGTGATTCAAGCACAAATTTCTCCACATCTACTCCTAGACAGAACGTGCTTGGTACACTCTGTCCTGAGGGGAGGGGGAATCTTTTGAGCATGTCCAGTGCTGGTCATTCAATGCCAACTGAGCAGTCACCTCAACAATCCTGGGATCCTAAGTATGCACATGGACAAGCCAATGCTTTTTCCAACTCAATGTCATTTCCACCATTTAATGGGAAAGACAACGCAGTTGAGCAAGAGAATTTAAATTCAGATACCCATAATCCTACTCTGTTTGGTGTTAATATTGAATCTTCTGGACTACTATTCCCAAACACTGTGCCCAGTTTTGCGACTTCGTCAAATGATGCTGACATTTCAATGCCTTTAGGGAACACAGGATTTCAGAGCTCTCTGTATGGTTGCATGCAAGACTCATCTGAGTTGTTGCACGGTGCAGGGCAAGTTGATCCACCAACCCCCAACAATTGTACATTTGTCAAGGTTTATAAGTCAGGTTCGGTTGGCCGCTCAATTGACATCTCCCGGTTCAGCAGCTATCATGAGCTGCGAGAAGAGCTGGGACAGATGTTTGGAATTGAGGGGAAGTTGGAATATCGTCTTAGATCAGGCTGGCAGCTTGTATTCGTTGACAGGGAGGATGATGTGCTTCTCCTTGGAGACGATCCATGGGAGTCATTTGTGAACAATGTTTGGTATATCAAGATACTTTCTCCCGAGGATGTGCACAAAATGGGGCACCAAGCAGTTGAATCCTTTAGCCCAAATACGGGTCAAAGGTTGAACAGCAGTGGCTGTGAAGGGCAAGATATTGTTTCAGGGCCACCGTCTCTTGGCTCACTTGAATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009908 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

842

Amino Acids

93.83

Weight (kDa)

5.86

Isoelectric Point (pI)

62.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	131 - 231	2.3e-21	B3 DNA binding domain
BRWD_AD	PF25313	255 - 363	9.8e-06	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	280 - 363	7.8e-34	Auxin response factor ancillary domain
AUX_IAA	PF02309	713 - 800	1.4e-07	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007603)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37020 AT5G37020 AT5G37020
fragaria_vesca	FvH4_1g27650 FvH4_1g27650 FvH4_1g27650
malus_domestica	MD09G1282200.v1.1 MD17G1272200.v1.1
prunus_persica	Prupe.3G011800_v2.0.a1
pyrus_communis	pycom09g19010 pycom17g27100
rosa_chinensis	RchiOBHm_Chr3g0487771
rosa_laevigata	RLG00000022974
rosa_multiflora	Rmu_sc0009101.1_g000014 Rmu_sc0040239.1_g000002 Rmu_sc0040567.1_g000004
rosa_roxburghii	Rroxscaffold_6G00394490
rosa_rugosa	Rorug03G0236300 Rorug03G0236400
rosa_samantha	Rh3AG284500 Rh3BG321300 Rh3CG319700 Rh3DG318200
rosa_wichuraiana	Rw3G025230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	813, 2163
AarI	CACCTGC	1 cut(s)	1564
AasI	GACNNNNNNGTC	2 cut(s)	928, 1134
Acc16I	TGCGCA	1 cut(s)	1572
Acc36I	ACCTGC	1 cut(s)	1564
AccB1I	GGYRCC	1 cut(s)	2412
AccB7I	CCANNNNNTGG	1 cut(s)	1886
AccBSI	CCGCTC	1 cut(s)	2184
AccI	GTMKAC	1 cut(s)	141
AccII	CGCG	1 cut(s)	98
AccIII	TCCGGA	1 cut(s)	906
AciI	CCGC	5 cut(s)	98, 149, 323, 770, 2182
AclWI	GGATC	9 cut(s)	42, 531, 974, 987, 1374, 1817, 1830, 2117, 2333
AcoI	YGGCCR	2 cut(s)	992, 2179
AcsI	RAATTY	5 cut(s)	73, 997, 1693, 1915, 1921
AcuI	CTGAAG	4 cut(s)	945, 1026, 1214, 1660
AcvI	CACGTG	1 cut(s)	884
AcyI	GRCGYC	1 cut(s)	320
AdeI	CACNNNGTG	2 cut(s)	256, 452
AfaI	GTAC	6 cut(s)	424, 947, 955, 1322, 1732, 2146
AfiI	CCNNNNNNNGG	5 cut(s)	125, 1886, 2443, 2444, 2507
AflIII	ACRYGT	2 cut(s)	881, 883
AhlI	ACTAGT	1 cut(s)	419
AjiI	CACGTC	1 cut(s)	449
AjnI	CCWGG	5 cut(s)	124, 165, 975, 1147, 1817
AleI	CACNNNNGTG	1 cut(s)	882
AloI	GAACNNNNNNTCC	2 cut(s)	627, 659
Alw21I	GWGCWC	2 cut(s)	2066, 2403
Alw26I	GTCTC	4 cut(s)	272, 914, 2328, 2508
Alw44I	GTGCAC	1 cut(s)	2399
AlwI	GGATC	9 cut(s)	42, 531, 974, 987, 1374, 1817, 1830, 2117, 2333
AlwNI	CAGNNNCTG	1 cut(s)	2469
Ama87I	CYCGRG	1 cut(s)	2390
Aor13HI	TCCGGA	1 cut(s)	906
AoxI	GGCC	5 cut(s)	154, 992, 1152, 2179, 2495
ApaLI	GTGCAC	1 cut(s)	2399
ApoI	RAATTY	5 cut(s)	73, 997, 1693, 1915, 1921
Asp700I	GAANNNNTTC	1 cut(s)	342
AspLEI	GCGC	2 cut(s)	98, 1573
AspS9I	GGNCC	4 cut(s)	101, 154, 1152, 2495
AsuC2I	CCSGG	2 cut(s)	1425, 2200
AsuHPI	GGTGA	2 cut(s)	1058, 1797
AvaI	CYCGRG	1 cut(s)	2390
AvaII	GGWCC	1 cut(s)	101
AxyI	CCTNAGG	1 cut(s)	1743
BaeGI	GKGCMC	3 cut(s)	1998, 2403, 2415
BaeI	ACNNNNGTAYC	2 cut(s)	937, 970
BalI	TGGCCA	1 cut(s)	994
BamHI	GGATCC	2 cut(s)	979, 1822
BanI	GGYRCC	1 cut(s)	2412
BanII	GRGCYC	1 cut(s)	2066
BauI	CACGAG	1 cut(s)	132
BbrPI	CACGTG	1 cut(s)	884
BbsI	GAAGAC	2 cut(s)	714, 1220
Bbv12I	GWGCWC	2 cut(s)	2066, 2403
BccI	CCATC	5 cut(s)	838, 1117, 1641, 1657, 1663
BceAI	ACGGC	2 cut(s)	423, 703
BcgI	CGANNNNNNTGC	4 cut(s)	152, 186, 542, 576
BciT130I	CCWGG	5 cut(s)	126, 167, 977, 1149, 1819
BciVI	GTATCC	1 cut(s)	1116
BclI	TGATCA	1 cut(s)	1306
BcnI	CCSGG	2 cut(s)	1425, 2200
BcoDI	GTCTC	4 cut(s)	272, 914, 2328, 2508
BcuI	ACTAGT	1 cut(s)	419
BfaI	CTAG	6 cut(s)	420, 519, 825, 933, 1355, 1713
BfmI	CTRYAG	3 cut(s)	591, 1445, 1493
BfuAI	ACCTGC	1 cut(s)	1564
BfuI	GTATCC	1 cut(s)	1116
Bme1390I	CCNGG	7 cut(s)	126, 167, 977, 1149, 1425, 1819, 2200
Bme18I	GGWCC	1 cut(s)	101
BmeT110I	CYCGRG	1 cut(s)	2390
BmgBI	CACGTC	1 cut(s)	449
BmgT120I	GGNCC	4 cut(s)	101, 154, 1152, 2495
BmiI	GGNNCC	5 cut(s)	102, 981, 1153, 1824, 2414
BmrFI	CCNGG	7 cut(s)	126, 167, 977, 1149, 1425, 1819, 2200
BmrI	ACTGGG	2 cut(s)	226, 1992
BmuI	ACTGGG	2 cut(s)	226, 1992
BpiI	GAAGAC	2 cut(s)	714, 1220
BpmI	CTGGAG	3 cut(s)	483, 619, 1526
Bpu10I	CCTNAGC	2 cut(s)	504, 1202
BpuEI	CTTGAG	1 cut(s)	1595
BpuMI	CCSGG	2 cut(s)	1425, 2200
BsaAI	YACGTR	1 cut(s)	884
BsaBI	GATNNNNATC	1 cut(s)	1311
BsaHI	GRCGYC	1 cut(s)	320
BsaWI	WCCGGW	1 cut(s)	906
BsaXI	ACNNNNNCTCC	4 cut(s)	627, 657, 1693, 1723
Bsc4I	CCNNNNNNNGG	5 cut(s)	125, 1886, 2443, 2444, 2507
Bse1I	ACTGG	6 cut(s)	232, 483, 967, 1324, 1773, 1998
Bse21I	CCTNAGG	1 cut(s)	1743
Bse3DI	GCAATG	1 cut(s)	305
Bse8I	GATNNNNATC	1 cut(s)	1311
BseAI	TCCGGA	1 cut(s)	906
BseBI	CCWGG	5 cut(s)	126, 167, 977, 1149, 1819
BseGI	GGATG	7 cut(s)	903, 915, 1036, 1275, 1609, 2320, 2401
BseJI	GATNNNNATC	1 cut(s)	1311
BseLI	CCNNNNNNNGG	5 cut(s)	125, 1886, 2443, 2444, 2507
BseMI	GCAATG	1 cut(s)	305
BseMII	CTCAG	5 cut(s)	518, 825, 1734, 1788, 2085
BseNI	ACTGG	6 cut(s)	232, 483, 967, 1324, 1773, 1998
BseRI	GAGGAG	2 cut(s)	60, 63
BseSI	GKGCMC	3 cut(s)	1998, 2403, 2415
BseYI	CCCAGC	2 cut(s)	1214, 2233
BsgI	GTGCAG	2 cut(s)	1598, 2130
Bsh1236I	CGCG	1 cut(s)	98
BshFI	GGCC	5 cut(s)	156, 994, 1154, 2181, 2497
BshNI	GGYRCC	1 cut(s)	2412
BsiHKAI	GWGCWC	2 cut(s)	2066, 2403
BsiHKCI	CYCGRG	1 cut(s)	2390
BsiSI	CCGG	4 cut(s)	633, 907, 1424, 2200
BslFI	GGGAC	5 cut(s)	97, 114, 142, 1512, 2250
BslI	CCNNNNNNNGG	5 cut(s)	125, 1886, 2443, 2444, 2507
BsmAI	GTCTC	4 cut(s)	272, 914, 2328, 2508
BsmBI	CGTCTC	2 cut(s)	2328, 2508
BsmFI	GGGAC	5 cut(s)	97, 114, 142, 1512, 2250
BsnI	GGCC	5 cut(s)	156, 994, 1154, 2181, 2497
BsoBI	CYCGRG	1 cut(s)	2390
Bsp1286I	GDGCHC	4 cut(s)	1998, 2066, 2403, 2415
Bsp13I	TCCGGA	1 cut(s)	906
Bsp1407I	TGTACA	1 cut(s)	2144
Bsp19I	CCATGG	2 cut(s)	1265, 2342
BspACI	CCGC	5 cut(s)	98, 149, 323, 770, 2182
BspANI	GGCC	5 cut(s)	156, 994, 1154, 2181, 2497
BspCNI	CTCAG	5 cut(s)	517, 826, 1735, 1789, 2086
BspEI	TCCGGA	1 cut(s)	906
BspFNI	CGCG	1 cut(s)	98
BspHI	TCATGA	2 cut(s)	37, 2215
BspLI	GGNNCC	5 cut(s)	102, 981, 1153, 1824, 2414
BspMAI	CTGCAG	2 cut(s)	1449, 1497
BspMI	ACCTGC	1 cut(s)	1564
BspPI	GGATC	9 cut(s)	42, 531, 974, 987, 1374, 1817, 1830, 2117, 2333
BspQI	GCTCTTC	1 cut(s)	2223
BspT107I	GGYRCC	1 cut(s)	2412
BsrBI	CCGCTC	1 cut(s)	2184
BsrDI	GCAATG	1 cut(s)	305
BsrGI	TGTACA	1 cut(s)	2144
BsrI	ACTGG	6 cut(s)	232, 483, 967, 1324, 1773, 1998
BssNI	GRCGYC	1 cut(s)	320
BssSI	CACGAG	1 cut(s)	132
BssT1I	CCWWGG	6 cut(s)	818, 1058, 1139, 1265, 2329, 2342
Bst2BI	CACGAG	1 cut(s)	132
Bst2UI	CCWGG	5 cut(s)	126, 167, 977, 1149, 1819
Bst4CI	ACNGT	6 cut(s)	712, 805, 1116, 1993, 2108, 2502
Bst6I	CTCTTC	2 cut(s)	921, 2223
BstACI	GRCGYC	1 cut(s)	320
BstAUI	TGTACA	1 cut(s)	2144
BstBAI	YACGTR	1 cut(s)	884
BstC8I	GCNNGC	8 cut(s)	90, 94, 267, 374, 746, 1461, 2081, 2288
BstDEI	CTNAG	9 cut(s)	504, 834, 1202, 1373, 1743, 1797, 1827, 2094, 2276
BstDSI	CCRYGG	2 cut(s)	1265, 2342
BstF5I	GGATG	7 cut(s)	903, 915, 1036, 1275, 1609, 2320, 2401
BstFNI	CGCG	1 cut(s)	98
BstHHI	GCGC	2 cut(s)	98, 1573
BstMAI	GTCTC	4 cut(s)	272, 914, 2328, 2508
BstNI	CCWGG	5 cut(s)	126, 167, 977, 1149, 1819
BstNSI	RCATGY	5 cut(s)	92, 269, 748, 1771, 2083
BstSCI	CCNGG	7 cut(s)	124, 165, 975, 1147, 1423, 1817, 2198
BstSFI	CTRYAG	3 cut(s)	591, 1445, 1493
BstSLI	GKGCMC	3 cut(s)	1998, 2403, 2415
BstUI	CGCG	1 cut(s)	98
BstV2I	GAAGAC	2 cut(s)	714, 1220
BstX2I	RGATCY	3 cut(s)	523, 979, 1822
BstYI	RGATCY	3 cut(s)	523, 979, 1822
Bsu36I	CCTNAGG	1 cut(s)	1743
BsuI	GTATCC	1 cut(s)	1116
BsuRI	GGCC	5 cut(s)	156, 994, 1154, 2181, 2497
BtgI	CCRYGG	2 cut(s)	1265, 2342
BtrI	CACGTC	1 cut(s)	449
BtsCI	GGATG	7 cut(s)	903, 915, 1036, 1275, 1609, 2320, 2401
BtsI	GCAGTG	1 cut(s)	2470
BtsIMutI	CAGTG	6 cut(s)	476, 1112, 1684, 1780, 1989, 2470
BveI	ACCTGC	1 cut(s)	1564
Cac8I	GCNNGC	8 cut(s)	90, 94, 267, 374, 746, 1461, 2081, 2288
CaiI	CAGNNNCTG	1 cut(s)	2469
CciI	TCATGA	2 cut(s)	37, 2215
CfoI	GCGC	2 cut(s)	98, 1573
Cfr13I	GGNCC	4 cut(s)	101, 154, 1152, 2495
CseI	GACGC	1 cut(s)	328
CsiI	ACCWGGT	1 cut(s)	165
Csp6I	GTAC	6 cut(s)	423, 946, 954, 1321, 1731, 2145
CviQI	GTAC	6 cut(s)	423, 946, 954, 1321, 1731, 2145
DdeI	CTNAG	9 cut(s)	504, 834, 1202, 1373, 1743, 1797, 1827, 2094, 2276
DraI	TTTAAA	1 cut(s)	1920
DraIII	CACNNNGTG	2 cut(s)	256, 452
DrdI	GACNNNNNNGTC	2 cut(s)	928, 1134
DseDI	GACNNNNNNGTC	2 cut(s)	928, 1134
EaeI	YGGCCR	2 cut(s)	992, 2179
Eam1104I	CTCTTC	2 cut(s)	921, 2223
EarI	CTCTTC	2 cut(s)	921, 2223
Ecl136II	GAGCTC	1 cut(s)	2064
Eco130I	CCWWGG	6 cut(s)	818, 1058, 1139, 1265, 2329, 2342
Eco24I	GRGCYC	1 cut(s)	2066
Eco47I	GGWCC	1 cut(s)	101
Eco53kI	GAGCTC	1 cut(s)	2064
Eco57I	CTGAAG	4 cut(s)	945, 1026, 1214, 1660
Eco72I	CACGTG	1 cut(s)	884
Eco81I	CCTNAGG	1 cut(s)	1743
Eco88I	CYCGRG	1 cut(s)	2390
EcoICRI	GAGCTC	1 cut(s)	2064
EcoO109I	RGGNCCY	2 cut(s)	101, 1152
EcoRI	GAATTC	1 cut(s)	73
EcoRII	CCWGG	5 cut(s)	124, 165, 975, 1147, 1817
EcoT14I	CCWWGG	6 cut(s)	818, 1058, 1139, 1265, 2329, 2342
EcoT22I	ATGCAT	4 cut(s)	267, 750, 1167, 1530
EcoT38I	GRGCYC	1 cut(s)	2066
ErhI	CCWWGG	6 cut(s)	818, 1058, 1139, 1265, 2329, 2342
Esp3I	CGTCTC	2 cut(s)	2328, 2508
FalI	AAGNNNNNCTT	2 cut(s)	53, 85
FaqI	GGGAC	5 cut(s)	97, 114, 142, 1512, 2250
FauI	CCCGC	1 cut(s)	91
FbaI	TGATCA	1 cut(s)	1306
FblI	GTMKAC	1 cut(s)	141
FokI	GGATG	7 cut(s)	910, 922, 1043, 1282, 1616, 2327, 2408
FriOI	GRGCYC	1 cut(s)	2066
FspBI	CTAG	6 cut(s)	420, 519, 825, 933, 1355, 1713
FspI	TGCGCA	1 cut(s)	1572
GlaI	GCGC	2 cut(s)	97, 1572
GsaI	CCCAGC	2 cut(s)	1218, 2237
GsuI	CTGGAG	3 cut(s)	483, 619, 1526
HaeIII	GGCC	5 cut(s)	156, 994, 1154, 2181, 2497
HapII	CCGG	4 cut(s)	633, 907, 1424, 2200
HgaI	GACGC	1 cut(s)	328
HhaI	GCGC	2 cut(s)	98, 1573
Hin1I	GRCGYC	1 cut(s)	320
Hin6I	GCGC	2 cut(s)	96, 1571
HinP1I	GCGC	2 cut(s)	96, 1571
HincII	GTYRAC	2 cut(s)	1038, 2305
HindII	GTYRAC	2 cut(s)	1038, 2305
HindIII	AAGCTT	1 cut(s)	5
HpaII	CCGG	4 cut(s)	633, 907, 1424, 2200
HphI	GGTGA	2 cut(s)	1058, 1797
Hpy166II	GTNNAC	7 cut(s)	142, 425, 1038, 1733, 2305, 2359, 2401
Hpy188I	TCNGA	8 cut(s)	79, 835, 941, 1440, 1638, 1927, 2061, 2095
Hpy188III	TCNNGA	9 cut(s)	38, 506, 907, 1601, 1742, 1971, 2216, 2377, 2390
Hpy8I	GTNNAC	7 cut(s)	142, 425, 1038, 1733, 2305, 2359, 2401
Hpy99I	CGWCG	1 cut(s)	453
HpyAV	CCTTC	4 cut(s)	35, 936, 1133, 2468
HpyCH4III	ACNGT	6 cut(s)	712, 805, 1116, 1993, 2108, 2502
HpyCH4IV	ACGT	4 cut(s)	448, 883, 1135, 1722
HpyF3I	CTNAG	9 cut(s)	504, 834, 1202, 1373, 1743, 1797, 1827, 2094, 2276
HpySE526I	ACGT	4 cut(s)	448, 883, 1135, 1722
Hsp92I	GRCGYC	1 cut(s)	320
HspAI	GCGC	2 cut(s)	96, 1571
Kpn2I	TCCGGA	1 cut(s)	906
Ksp22I	TGATCA	1 cut(s)	1306
LguI	GCTCTTC	1 cut(s)	2223
LmnI	GCTCC	5 cut(s)	79, 252, 1211, 1219, 1635
MabI	ACCWGGT	1 cut(s)	165
MaeI	CTAG	6 cut(s)	420, 519, 825, 933, 1355, 1713
MaeII	ACGT	4 cut(s)	448, 883, 1135, 1722
MaeIII	GTNAC	4 cut(s)	256, 971, 1449, 1803
MbiI	CCGCTC	1 cut(s)	2184
MboII	GAAGA	7 cut(s)	338, 575, 714, 938, 1220, 1959, 2240
MfeI	CAATTG	2 cut(s)	2140, 2187
MflI	RGATCY	3 cut(s)	523, 979, 1822
MhlI	GDGCHC	4 cut(s)	1998, 2066, 2403, 2415
MlsI	TGGCCA	1 cut(s)	994
MluNI	TGGCCA	1 cut(s)	994
MlyI	GAGTC	6 cut(s)	124, 938, 1043, 1648, 2081, 2357
MmeI	TCCRAC	2 cut(s)	1884, 2244
Mox20I	TGGCCA	1 cut(s)	994
Mph1103I	ATGCAT	4 cut(s)	267, 750, 1167, 1530
MroI	TCCGGA	1 cut(s)	906
MroXI	GAANNNNTTC	1 cut(s)	342
MscI	TGGCCA	1 cut(s)	994
MseI	TTAA	7 cut(s)	236, 864, 1193, 1554, 1884, 1919, 1956
MslI	CAYNNNNRTG	3 cut(s)	270, 882, 2033
Msp20I	TGGCCA	1 cut(s)	994
MspA1I	CMGCKG	1 cut(s)	767
MspI	CCGG	4 cut(s)	633, 907, 1424, 2200
MspR9I	CCNGG	7 cut(s)	126, 167, 977, 1149, 1425, 1819, 2200
MunI	CAATTG	2 cut(s)	2140, 2187
MvaI	CCWGG	5 cut(s)	126, 167, 977, 1149, 1819
MvnI	CGCG	1 cut(s)	98
NciI	CCSGG	2 cut(s)	1425, 2200
NcoI	CCATGG	2 cut(s)	1265, 2342
NlaIV	GGNNCC	5 cut(s)	102, 981, 1153, 1824, 2414
NmeAIII	GCCGAG	1 cut(s)	484
NmuCI	GTSAC	3 cut(s)	256, 971, 1803
NsbI	TGCGCA	1 cut(s)	1572
NsiI	ATGCAT	4 cut(s)	267, 750, 1167, 1530
NspI	RCATGY	5 cut(s)	92, 269, 748, 1771, 2083
OliI	CACNNNNGTG	1 cut(s)	882
PaeI	GCATGC	4 cut(s)	92, 269, 748, 2083
PagI	TCATGA	2 cut(s)	37, 2215
PaqCI	CACCTGC	1 cut(s)	1564
PciSI	GCTCTTC	1 cut(s)	2223
PcsI	WCGNNNNNNNCGW	1 cut(s)	1132
PdmI	GAANNNNTTC	1 cut(s)	342
PflFI	GACNNNGTC	1 cut(s)	2011
PflMI	CCANNNNNTGG	1 cut(s)	1886
PleI	GAGTC	6 cut(s)	124, 937, 1042, 1647, 2081, 2356
PmaCI	CACGTG	1 cut(s)	884
PmlI	CACGTG	1 cut(s)	884
PpsI	GAGTC	6 cut(s)	124, 937, 1042, 1647, 2081, 2356
Ppu21I	YACGTR	1 cut(s)	884
PpuMI	RGGWCCY	1 cut(s)	101
PsiI	TTATAA	2 cut(s)	813, 2163
Psp124BI	GAGCTC	1 cut(s)	2066
Psp5II	RGGWCCY	1 cut(s)	101
Psp6I	CCWGG	5 cut(s)	124, 165, 975, 1147, 1817
PspCI	CACGTG	1 cut(s)	884
PspFI	CCCAGC	2 cut(s)	1214, 2233
PspGI	CCWGG	5 cut(s)	124, 165, 975, 1147, 1817
PspN4I	GGNNCC	5 cut(s)	102, 981, 1153, 1824, 2414
PspPI	GGNCC	4 cut(s)	101, 154, 1152, 2495
PspPPI	RGGWCCY	1 cut(s)	101
PstI	CTGCAG	2 cut(s)	1449, 1497
PstNI	CAGNNNCTG	1 cut(s)	2469
PsuI	RGATCY	3 cut(s)	523, 979, 1822
PsyI	GACNNNGTC	1 cut(s)	2011
PvuII	CAGCTG	1 cut(s)	767
RsaI	GTAC	6 cut(s)	424, 947, 955, 1322, 1732, 2146
RsaNI	GTAC	6 cut(s)	423, 946, 954, 1321, 1731, 2145
RseI	CAYNNNNRTG	3 cut(s)	270, 882, 2033
SacI	GAGCTC	1 cut(s)	2066
SapI	GCTCTTC	1 cut(s)	2223
SaqAI	TTAA	7 cut(s)	236, 864, 1193, 1554, 1884, 1919, 1956
Sau96I	GGNCC	4 cut(s)	101, 154, 1152, 2495
SbfI	CCTGCAGG	1 cut(s)	1497
SchI	GAGTC	6 cut(s)	124, 938, 1043, 1648, 2081, 2357
ScrFI	CCNGG	7 cut(s)	126, 167, 977, 1149, 1425, 1819, 2200
SdaI	CCTGCAGG	1 cut(s)	1497
SduI	GDGCHC	4 cut(s)	1998, 2066, 2403, 2415
SexAI	ACCWGGT	1 cut(s)	165
SfcI	CTRYAG	3 cut(s)	591, 1445, 1493
SinI	GGWCC	1 cut(s)	101
SmiI	ATTTAAAT	1 cut(s)	1920
SmiMI	CAYNNNNRTG	3 cut(s)	270, 882, 2033
SmlI	CTYRAG	1 cut(s)	1610
SmoI	CTYRAG	1 cut(s)	1610
SpeI	ACTAGT	1 cut(s)	419
SphI	GCATGC	4 cut(s)	92, 269, 748, 2083
Sse8387I	CCTGCAGG	1 cut(s)	1497
SsiI	CCGC	5 cut(s)	98, 149, 323, 770, 2182
SspI	AATATT	2 cut(s)	1960, 2522
SspMI	CTAG	6 cut(s)	420, 519, 825, 933, 1355, 1713
SstI	GAGCTC	1 cut(s)	2066
StyD4I	CCNGG	7 cut(s)	124, 165, 975, 1147, 1423, 1817, 2198
StyI	CCWWGG	6 cut(s)	818, 1058, 1139, 1265, 2329, 2342
SwaI	ATTTAAAT	1 cut(s)	1920
TaaI	ACNGT	6 cut(s)	712, 805, 1116, 1993, 2108, 2502
TaiI	ACGT	4 cut(s)	451, 886, 1138, 1725
TaqI	TCGA	3 cut(s)	562, 700, 1126
TatI	WGTACW	2 cut(s)	422, 2144
TauI	GCSGC	3 cut(s)	325, 773, 2184
Tru1I	TTAA	7 cut(s)	236, 864, 1193, 1554, 1884, 1919, 1956
Tru9I	TTAA	7 cut(s)	236, 864, 1193, 1554, 1884, 1919, 1956
TscAI	CASTG	6 cut(s)	483, 1119, 1684, 1780, 1996, 2470
TseFI	GTSAC	3 cut(s)	256, 971, 1803
Tsp45I	GTSAC	3 cut(s)	256, 971, 1803
TspDTI	ATGAA	7 cut(s)	17, 54, 300, 675, 1194, 1253, 1638
TspGWI	ACGGA	1 cut(s)	444
TspRI	CASTG	6 cut(s)	483, 1119, 1684, 1780, 1996, 2470
Tth111I	GACNNNGTC	1 cut(s)	2011
Van91I	CCANNNNNTGG	1 cut(s)	1886
VneI	GTGCAC	1 cut(s)	2399
VpaK11BI	GGWCC	1 cut(s)	101
XapI	RAATTY	5 cut(s)	73, 997, 1693, 1915, 1921
XceI	RCATGY	5 cut(s)	92, 269, 748, 1771, 2083
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1002
XmiI	GTMKAC	1 cut(s)	141
XmnI	GAANNNNTTC	1 cut(s)	342
XspI	CTAG	6 cut(s)	420, 519, 825, 933, 1355, 1713
Zsp2I	ATGCAT	4 cut(s)	267, 750, 1167, 1530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.