Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007603

Physical Location & Seq

Genomic Coordinates Forward (+)

33417467 .. 33427853

Gene Structure 10387 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1272200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGAAGCTTTCAGCATCAGGGCTGGGTCAGCAGGATCATGAAGGAGGGGGAGGAGGAGGAGTGGAAAAGAAGTGCTTGAATTCGGAGCTGTGGCATGCGTGCGCTGGGCCTCTGGTATCCCTGCCGACACGTGGGACTCGTGTGGTCTACTTTCCGCAGGGCCACAGCGACCAGGTTGCCGCCACTACCAACAAGCAAATTGATGCCCACCTACCAAACTACCCGAGCTTGCCCCCCCAGTTGATCTGTCAGCTCCATAATATCACCATGCATGCAGATGTGGAGACAGATGAAGTATATGCCCAAATGACATTGCAGCCTTTGACGCCGCAAGAACAAAAAGAAACATTTCTTCCAATGGAGTTGGGGCTTCCAAGCAAGCAACCCACAAACTACTTTTGCAAGACATTGACCGCAAGTGATACTAGTACACATGGTGGCTTCTCTGTTCCTCGTCGTGCTGCTGAGAAAGTTTTTCCTCCACTTGATTTTACGCTACAACCACCAGCTCAGGAACTTATTGCTAGGGATCTCCATGATGTTGAGTGGAAGTTTAGGCATATTTTTCGAGGACAGCCGAAACGACATCTTCTTACTACAGGCTGGAGTGTGTTTGTTAGTGCCAAAAGACTTGTTGCTGGAGATTCTGTTCTTTTTATTTGGAATGAAAAGAATCAGCTTTTTTTGGGGATTCGTCGTGCCACTCGACCACAAACTGTGATGCCATCTTCTGTTTTGTCAAGTGATAGCATGCATATTGGACTTCTTGCTGCTGCGGCTCATGCAGCTTCAACTAACAGCTGTTTTACAGTTTTTTATAATCCAAGGGCTAGTCCATCTGAGTTTGTCATACCCCTATCAAAATACATTAAAGCAGTATTTCACACACGTGTTTCAGTTGGGATGCGATTCCGGATGCTATTTGAGACTGAAGAGTCTAGTGTCAGAAGGTACATGGGTACGATAACTGGCATAAGTGATCTGGATCCTGTTCGGTGGCCAAATTCTCATTGGCGATCTGTCAAGGTTGGTTGGGATGAGTCTACTGCAGGTGAGAGGCAACCAAGGGTATCATTATGGGAGATTGAGCCTTTGACAACTTTCCCCATGTACCCATCACTGTTTCCCCTTCGACTGAAACGTCCTTGGCATCCTGGGGCCTCATCTATGCATGATAATAGAGATGAATCTAATAGCTTAATGTGGCTAAGGGGGGGAGCTGTAGAGCAAGGTCTTCAGTCCATGAACTTTCAAAATGTTGGTATGTTTCCATGGATGCAACAGAGATTGGATTCGACATTGATTGGAAATGATCACAATCAGCAGTACCAGGCCATGTTGGCTGCTGGTTCGCAGAATCTAGGAAGTGGAGATCAGCTAAGACAACAGATGATGCACTTTCAGCAGCCTTTTCAGTATGTTCAGCAGTCGGGTAGCCACAATCCGATGCTGCAGTTACAACAGCAAGCAATTCAGCAGTCAATCCCTCATAATATCCTGCAGGGACAGCAACAAGTTTCAATGGAGAATATGCCTCAGCACCTTCTCCAGCAACAATTTAACAATCAAACAGATGAGCAGGCTCAGCAACACCAAAACGCCTATCAGGATGCACTCAAGGTTCAAAATGAACAACTCCATCAGAGTCAGCAAATGAATGTGTCATCTACGTCGTTCCTGAAAACAGACTTAAGTGATTCAAGCACAAAGTTCTCAGCATCTACTACTCCTAGACAGAACATGCTTGGTACACTGTGTCCTGATGGGAGCGGAAATCTTTTGAGCATGTCCAGAGCTGGTCATTCAATGCCAACTGAGCAGTCACCTCAGCAATCCTGGGCTCCAAAGTATGCACATGGACAGGCCAATGCTTTTGCCAACTCAATGTCATTTCCACCATTTAATGAGAAAGACAATGCAGTTGAGCAAGAGAATTTAAATTCAGATACCCATAATCCTACTCTGTTTGGTGTTAATATTGAATCTTCTGGACTTCTATTCCCAACCAGTGCGCCCAGTTTTGCGACTTCCTCAAATGATGCTGACATTTCAATGCCTTTAGGGGACTCGGGACTTCAGAGATCTCTGTATGGTTGCATGCAAGACTCTTCTGAGTTGTTGCACAGTGCAGGGCAAGTTGATCCACCGACCCCCAACAATTGTACATTTGTCAAGGTTTATAAGTCAGGGTCAGTTGGCCGCTCACTGGACATATCCCGGTTCAGCAGCTATCATGAGCTGCGAGAAGAGCTGGGACAGATGTTTGGGATTGAGGGGAAGTTGGAGGATCGTCTTAGATCAGGCTGGCAGCTTGTATTCGTTGACAGGGAGGATGATGTGCTTCTCCTTGGAGACGACCCATGGGAGTCTTTTGTGAACAATGTTTGGTATATCAAGATACTTTCTCCTGAGGATGTGCACAAAATGGGGCACCAAGCAGTTGAATCCTTTAGCCCAAATACAGGTCAAAGGTTGAACAGCGGTGGTGGCGAAGGTCAAGACATTGTTTCAGGGCTACCATCTCTCGGCTCACTTGAATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009908 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

848

Amino Acids

94.42

Weight (kDa)

5.91

Isoelectric Point (pI)

64.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	133 - 234	6.8e-22	B3 DNA binding domain
ARF_AD	PF06507	282 - 365	7.6e-34	Auxin response factor ancillary domain
AUX_IAA	PF02309	718 - 806	1.3e-07	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007603)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37020 AT5G37020 AT5G37020
fragaria_vesca	FvH4_1g27650 FvH4_1g27650 FvH4_1g27650
malus_domestica	MD09G1282200.v1.1 MD17G1272200.v1.1
prunus_persica	Prupe.3G011800_v2.0.a1
pyrus_communis	pycom09g19010 pycom17g27100
rosa_chinensis	RchiOBHm_Chr3g0487771
rosa_laevigata	RLG00000022974
rosa_multiflora	Rmu_sc0009101.1_g000014 Rmu_sc0040239.1_g000002 Rmu_sc0040567.1_g000004
rosa_roxburghii	Rroxscaffold_6G00394490
rosa_rugosa	Rorug03G0236300 Rorug03G0236400
rosa_samantha	Rh3AG284500 Rh3BG321300 Rh3CG319700 Rh3DG318200
rosa_wichuraiana	Rw3G025230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	819, 2181
AarI	CACCTGC	1 cut(s)	1040
AasI	GACNNNNNNGTC	2 cut(s)	934, 1140
Acc36I	ACCTGC	1 cut(s)	1040
AccB1I	GGYRCC	1 cut(s)	2430
AccBSI	CCGCTC	2 cut(s)	1770, 2202
AccI	GTMKAC	2 cut(s)	147, 1043
AccIII	TCCGGA	1 cut(s)	912
AciI	CCGC	8 cut(s)	155, 180, 329, 414, 776, 1770, 2200, 2481
AclWI	GGATC	6 cut(s)	42, 537, 980, 993, 2135, 2295
AcoI	YGGCCR	2 cut(s)	998, 2197
AcsI	RAATTY	4 cut(s)	79, 1003, 1933, 1939
AcuI	CTGAAG	3 cut(s)	951, 1220, 2060
AcvI	CACGTG	2 cut(s)	131, 890
AcyI	GRCGYC	1 cut(s)	326
AdeI	CACNNNGTG	1 cut(s)	437
AfaI	GTAC	7 cut(s)	430, 953, 961, 1112, 1328, 1750, 2164
AfiI	CCNNNNNNNGG	4 cut(s)	131, 2206, 2462, 2525
AflII	CTTAAG	1 cut(s)	1690
AflIII	ACRYGT	3 cut(s)	128, 887, 889
AhlI	ACTAGT	1 cut(s)	425
AjnI	CCWGG	4 cut(s)	171, 1153, 1329, 1835
AleI	CACNNNNGTG	1 cut(s)	888
AloI	GAACNNNNNNTCC	2 cut(s)	633, 665
Alw21I	GWGCWC	1 cut(s)	2421
Alw26I	GTCTC	3 cut(s)	278, 920, 2346
Alw44I	GTGCAC	1 cut(s)	2417
AlwI	GGATC	6 cut(s)	42, 537, 980, 993, 2135, 2295
Ama87I	CYCGRG	2 cut(s)	223, 2068
Aor13HI	TCCGGA	1 cut(s)	912
AoxI	GGCC	7 cut(s)	107, 160, 998, 1158, 1332, 1863, 2197
ApaLI	GTGCAC	1 cut(s)	2417
ApoI	RAATTY	4 cut(s)	79, 1003, 1933, 1939
Asp700I	GAANNNNTTC	1 cut(s)	348
AspLEI	GCGC	2 cut(s)	104, 2014
AspS9I	GGNCC	3 cut(s)	107, 160, 1158
AsuC2I	CCSGG	1 cut(s)	2218
AsuHPI	GGTGA	3 cut(s)	256, 1064, 1815
AvaI	CYCGRG	2 cut(s)	223, 2068
AxyI	CCTNAGG	1 cut(s)	2409
BaeGI	GKGCMC	2 cut(s)	2421, 2433
BaeI	ACNNNNGTAYC	2 cut(s)	943, 976
BalI	TGGCCA	1 cut(s)	1000
BamHI	GGATCC	1 cut(s)	985
BanI	GGYRCC	1 cut(s)	2430
BanII	GRGCYC	1 cut(s)	1843
BauI	CACGAG	1 cut(s)	138
BbrPI	CACGTG	2 cut(s)	131, 890
BbsI	GAAGAC	1 cut(s)	1226
Bbv12I	GWGCWC	1 cut(s)	2421
BbvCI	CCTCAGC	2 cut(s)	1536, 1827
BccI	CCATC	6 cut(s)	733, 844, 1123, 1647, 1757, 2527
BcgI	CGANNNNNNTGC	4 cut(s)	158, 192, 548, 582
BciT130I	CCWGG	4 cut(s)	173, 1155, 1331, 1837
BciVI	GTATCC	1 cut(s)	127
BclI	TGATCA	1 cut(s)	1312
BcnI	CCSGG	1 cut(s)	2218
BcoDI	GTCTC	3 cut(s)	278, 920, 2346
BcuI	ACTAGT	1 cut(s)	425
BfaI	CTAG	6 cut(s)	426, 525, 831, 939, 1361, 1731
BfmI	CTRYAG	5 cut(s)	597, 1047, 1221, 1451, 1499
BfrI	CTTAAG	1 cut(s)	1690
BfuAI	ACCTGC	1 cut(s)	1040
BfuI	GTATCC	1 cut(s)	127
BglI	GCCNNNNNGGC	1 cut(s)	1340
BglII	AGATCT	1 cut(s)	2081
BlpI	GCTNAGC	1 cut(s)	1584
Bme1390I	CCNGG	5 cut(s)	173, 1155, 1331, 1837, 2218
BmeT110I	CYCGRG	2 cut(s)	223, 2068
BmgT120I	GGNCC	3 cut(s)	107, 160, 1158
BmiI	GGNNCC	4 cut(s)	987, 1159, 1842, 2432
BmrFI	CCNGG	5 cut(s)	173, 1155, 1331, 1837, 2218
BmrI	ACTGGG	2 cut(s)	232, 2010
BmuI	ACTGGG	2 cut(s)	232, 2010
BpiI	GAAGAC	1 cut(s)	1226
BpmI	CTGGAG	3 cut(s)	625, 660, 1532
Bpu10I	CCTNAGC	4 cut(s)	510, 1208, 1536, 1827
Bpu1102I	GCTNAGC	1 cut(s)	1584
BpuEI	CTTGAG	1 cut(s)	1601
BpuMI	CCSGG	1 cut(s)	2218
BsaAI	YACGTR	2 cut(s)	131, 890
BsaBI	GATNNNNATC	2 cut(s)	984, 1317
BsaHI	GRCGYC	1 cut(s)	326
BsaJI	CCNNGG	8 cut(s)	824, 1064, 1145, 1154, 1271, 1836, 2347, 2360
BsaWI	WCCGGW	1 cut(s)	912
BsaXI	ACNNNNNCTCC	4 cut(s)	45, 75, 633, 663
Bsc4I	CCNNNNNNNGG	4 cut(s)	131, 2206, 2462, 2525
Bse1I	ACTGG	5 cut(s)	238, 973, 2007, 2016, 2211
Bse21I	CCTNAGG	1 cut(s)	2409
Bse3DI	GCAATG	1 cut(s)	311
Bse8I	GATNNNNATC	2 cut(s)	984, 1317
BseAI	TCCGGA	1 cut(s)	912
BseBI	CCWGG	4 cut(s)	173, 1155, 1331, 1837
BseDI	CCNNGG	8 cut(s)	824, 1064, 1145, 1154, 1271, 1836, 2347, 2360
BseGI	GGATG	8 cut(s)	909, 921, 1042, 1150, 1281, 1615, 2338, 2419
BseJI	GATNNNNATC	2 cut(s)	984, 1317
BseLI	CCNNNNNNNGG	4 cut(s)	131, 2206, 2462, 2525
BseMI	GCAATG	1 cut(s)	311
BseNI	ACTGG	5 cut(s)	238, 973, 2007, 2016, 2211
BseRI	GAGGAG	3 cut(s)	66, 69, 72
BseSI	GKGCMC	2 cut(s)	2421, 2433
BseYI	CCCAGC	3 cut(s)	22, 104, 2251
BsgI	GTGCAG	1 cut(s)	2148
BshFI	GGCC	7 cut(s)	109, 162, 1000, 1160, 1334, 1865, 2199
BshNI	GGYRCC	1 cut(s)	2430
BsiHKAI	GWGCWC	1 cut(s)	2421
BsiHKCI	CYCGRG	2 cut(s)	223, 2068
BsiSI	CCGG	2 cut(s)	913, 2218
BslFI	GGGAC	5 cut(s)	148, 1518, 2078, 2085, 2268
BslI	CCNNNNNNNGG	4 cut(s)	131, 2206, 2462, 2525
BsmAI	GTCTC	3 cut(s)	278, 920, 2346
BsmBI	CGTCTC	1 cut(s)	2346
BsmFI	GGGAC	5 cut(s)	148, 1518, 2078, 2085, 2268
BsnI	GGCC	7 cut(s)	109, 162, 1000, 1160, 1334, 1865, 2199
BsoBI	CYCGRG	2 cut(s)	223, 2068
Bsp1286I	GDGCHC	3 cut(s)	1843, 2421, 2433
Bsp13I	TCCGGA	1 cut(s)	912
Bsp1407I	TGTACA	1 cut(s)	2162
Bsp1720I	GCTNAGC	1 cut(s)	1584
Bsp19I	CCATGG	2 cut(s)	1271, 2360
BspACI	CCGC	8 cut(s)	155, 180, 329, 414, 776, 1770, 2200, 2481
BspANI	GGCC	7 cut(s)	109, 162, 1000, 1160, 1334, 1865, 2199
BspEI	TCCGGA	1 cut(s)	912
BspHI	TCATGA	2 cut(s)	37, 2233
BspLI	GGNNCC	4 cut(s)	987, 1159, 1842, 2432
BspMAI	CTGCAG	3 cut(s)	1051, 1455, 1503
BspMI	ACCTGC	1 cut(s)	1040
BspPI	GGATC	6 cut(s)	42, 537, 980, 993, 2135, 2295
BspQI	GCTCTTC	1 cut(s)	2241
BspT107I	GGYRCC	1 cut(s)	2430
BspTI	CTTAAG	1 cut(s)	1690
BsrBI	CCGCTC	2 cut(s)	1770, 2202
BsrDI	GCAATG	1 cut(s)	311
BsrGI	TGTACA	1 cut(s)	2162
BsrI	ACTGG	5 cut(s)	238, 973, 2007, 2016, 2211
BssECI	CCNNGG	8 cut(s)	824, 1064, 1145, 1154, 1271, 1836, 2347, 2360
BssNI	GRCGYC	1 cut(s)	326
BssSI	CACGAG	1 cut(s)	138
BssT1I	CCWWGG	6 cut(s)	824, 1064, 1145, 1271, 2347, 2360
Bst2BI	CACGAG	1 cut(s)	138
Bst2UI	CCWGG	4 cut(s)	173, 1155, 1331, 1837
Bst4CI	ACNGT	5 cut(s)	718, 811, 1122, 1755, 2126
Bst6I	CTCTTC	3 cut(s)	927, 2113, 2241
BstACI	GRCGYC	1 cut(s)	326
BstAFI	CTTAAG	1 cut(s)	1690
BstAUI	TGTACA	1 cut(s)	2162
BstBAI	YACGTR	2 cut(s)	131, 890
BstDSI	CCRYGG	2 cut(s)	1271, 2360
BstF5I	GGATG	8 cut(s)	909, 921, 1042, 1150, 1281, 1615, 2338, 2419
BstHHI	GCGC	2 cut(s)	104, 2014
BstMAI	GTCTC	3 cut(s)	278, 920, 2346
BstNI	CCWGG	4 cut(s)	173, 1155, 1331, 1837
BstNSI	RCATGY	6 cut(s)	98, 275, 754, 1744, 1789, 2101
BstSCI	CCNGG	5 cut(s)	171, 1153, 1329, 1835, 2216
BstSFI	CTRYAG	5 cut(s)	597, 1047, 1221, 1451, 1499
BstSLI	GKGCMC	2 cut(s)	2421, 2433
BstV2I	GAAGAC	1 cut(s)	1226
BstX2I	RGATCY	3 cut(s)	529, 985, 2081
BstYI	RGATCY	3 cut(s)	529, 985, 2081
Bsu36I	CCTNAGG	1 cut(s)	2409
BsuI	GTATCC	1 cut(s)	127
BsuRI	GGCC	7 cut(s)	109, 162, 1000, 1160, 1334, 1865, 2199
BtgI	CCRYGG	2 cut(s)	1271, 2360
BtsCI	GGATG	8 cut(s)	909, 921, 1042, 1150, 1281, 1615, 2338, 2419
BtsIMutI	CAGTG	5 cut(s)	1118, 1751, 2014, 2131, 2204
BveI	ACCTGC	1 cut(s)	1040
CciI	TCATGA	2 cut(s)	37, 2233
CfoI	GCGC	2 cut(s)	104, 2014
Cfr13I	GGNCC	3 cut(s)	107, 160, 1158
CseI	GACGC	1 cut(s)	334
CsiI	ACCWGGT	1 cut(s)	171
Csp6I	GTAC	7 cut(s)	429, 952, 960, 1111, 1327, 1749, 2163
CviQI	GTAC	7 cut(s)	429, 952, 960, 1111, 1327, 1749, 2163
DraI	TTTAAA	1 cut(s)	1938
DraIII	CACNNNGTG	1 cut(s)	437
DrdI	GACNNNNNNGTC	2 cut(s)	934, 1140
DseDI	GACNNNNNNGTC	2 cut(s)	934, 1140
EaeI	YGGCCR	2 cut(s)	998, 2197
Eam1104I	CTCTTC	3 cut(s)	927, 2113, 2241
EarI	CTCTTC	3 cut(s)	927, 2113, 2241
Eco130I	CCWWGG	6 cut(s)	824, 1064, 1145, 1271, 2347, 2360
Eco24I	GRGCYC	1 cut(s)	1843
Eco57I	CTGAAG	3 cut(s)	951, 1220, 2060
Eco72I	CACGTG	2 cut(s)	131, 890
Eco81I	CCTNAGG	1 cut(s)	2409
Eco88I	CYCGRG	2 cut(s)	223, 2068
EcoO109I	RGGNCCY	1 cut(s)	1158
EcoRI	GAATTC	1 cut(s)	79
EcoRII	CCWGG	4 cut(s)	171, 1153, 1329, 1835
EcoT14I	CCWWGG	6 cut(s)	824, 1064, 1145, 1271, 2347, 2360
EcoT22I	ATGCAT	3 cut(s)	273, 756, 1173
EcoT38I	GRGCYC	1 cut(s)	1843
ErhI	CCWWGG	6 cut(s)	824, 1064, 1145, 1271, 2347, 2360
Esp3I	CGTCTC	1 cut(s)	2346
FalI	AAGNNNNNCTT	2 cut(s)	59, 91
FaqI	GGGAC	5 cut(s)	148, 1518, 2078, 2085, 2268
FbaI	TGATCA	1 cut(s)	1312
FblI	GTMKAC	2 cut(s)	147, 1043
FokI	GGATG	8 cut(s)	916, 928, 1049, 1137, 1288, 1622, 2345, 2426
FriOI	GRGCYC	1 cut(s)	1843
FspBI	CTAG	6 cut(s)	426, 525, 831, 939, 1361, 1731
GlaI	GCGC	2 cut(s)	103, 2013
GsaI	CCCAGC	3 cut(s)	26, 108, 2255
GsuI	CTGGAG	3 cut(s)	625, 660, 1532
HaeIII	GGCC	7 cut(s)	109, 162, 1000, 1160, 1334, 1865, 2199
HapII	CCGG	2 cut(s)	913, 2218
HgaI	GACGC	1 cut(s)	334
HhaI	GCGC	2 cut(s)	104, 2014
Hin1I	GRCGYC	1 cut(s)	326
Hin6I	GCGC	2 cut(s)	102, 2012
HinP1I	GCGC	2 cut(s)	102, 2012
HincII	GTYRAC	1 cut(s)	2323
HindII	GTYRAC	1 cut(s)	2323
HindIII	AAGCTT	1 cut(s)	5
HpaII	CCGG	2 cut(s)	913, 2218
HphI	GGTGA	3 cut(s)	256, 1064, 1815
Hpy166II	GTNNAC	7 cut(s)	148, 431, 1044, 1751, 2323, 2377, 2419
Hpy188I	TCNGA	8 cut(s)	85, 841, 947, 1446, 1644, 1945, 2079, 2113
Hpy8I	GTNNAC	7 cut(s)	148, 431, 1044, 1751, 2323, 2377, 2419
Hpy99I	CGWCG	3 cut(s)	459, 699, 1675
HpyAV	CCTTC	5 cut(s)	35, 942, 1139, 1553, 2486
HpyCH4III	ACNGT	5 cut(s)	718, 811, 1122, 1755, 2126
HpyCH4IV	ACGT	4 cut(s)	130, 889, 1141, 1670
HpySE526I	ACGT	4 cut(s)	130, 889, 1141, 1670
Hsp92I	GRCGYC	1 cut(s)	326
HspAI	GCGC	2 cut(s)	102, 2012
Kpn2I	TCCGGA	1 cut(s)	912
Ksp22I	TGATCA	1 cut(s)	1312
LguI	GCTCTTC	1 cut(s)	2241
LmnI	GCTCC	5 cut(s)	85, 258, 1217, 1767, 1846
MabI	ACCWGGT	1 cut(s)	171
MaeI	CTAG	6 cut(s)	426, 525, 831, 939, 1361, 1731
MaeII	ACGT	4 cut(s)	130, 889, 1141, 1670
MaeIII	GTNAC	2 cut(s)	1455, 1821
MbiI	CCGCTC	2 cut(s)	1770, 2202
MboII	GAAGA	8 cut(s)	344, 581, 720, 944, 1226, 1977, 2100, 2258
MfeI	CAATTG	1 cut(s)	2158
MflI	RGATCY	3 cut(s)	529, 985, 2081
MhlI	GDGCHC	3 cut(s)	1843, 2421, 2433
MlsI	TGGCCA	1 cut(s)	1000
MluCI	AATT	8 cut(s)	79, 198, 1003, 1470, 1556, 1933, 1939, 2158
MluNI	TGGCCA	1 cut(s)	1000
MlyI	GAGTC	7 cut(s)	130, 944, 1049, 1654, 2060, 2099, 2375
MmeI	TCCRAC	1 cut(s)	2262
Mox20I	TGGCCA	1 cut(s)	1000
Mph1103I	ATGCAT	3 cut(s)	273, 756, 1173
MroI	TCCGGA	1 cut(s)	912
MroXI	GAANNNNTTC	1 cut(s)	348
MscI	TGGCCA	1 cut(s)	1000
MseI	TTAA	7 cut(s)	870, 1199, 1560, 1691, 1902, 1937, 1974
MslI	CAYNNNNRTG	3 cut(s)	276, 888, 2051
Msp20I	TGGCCA	1 cut(s)	1000
MspA1I	CMGCKG	2 cut(s)	801, 2481
MspCI	CTTAAG	1 cut(s)	1690
MspI	CCGG	2 cut(s)	913, 2218
MspR9I	CCNGG	5 cut(s)	173, 1155, 1331, 1837, 2218
MunI	CAATTG	1 cut(s)	2158
MvaI	CCWGG	4 cut(s)	173, 1155, 1331, 1837
NciI	CCSGG	1 cut(s)	2218
NcoI	CCATGG	2 cut(s)	1271, 2360
NlaIV	GGNNCC	4 cut(s)	987, 1159, 1842, 2432
NmeAIII	GCCGAG	1 cut(s)	2505
NmuCI	GTSAC	1 cut(s)	1821
NsiI	ATGCAT	3 cut(s)	273, 756, 1173
NspI	RCATGY	6 cut(s)	98, 275, 754, 1744, 1789, 2101
OliI	CACNNNNGTG	1 cut(s)	888
PaeI	GCATGC	4 cut(s)	98, 275, 754, 2101
PagI	TCATGA	2 cut(s)	37, 2233
PaqCI	CACCTGC	1 cut(s)	1040
PciSI	GCTCTTC	1 cut(s)	2241
PcsI	WCGNNNNNNNCGW	3 cut(s)	136, 703, 1138
PdmI	GAANNNNTTC	1 cut(s)	348
PleI	GAGTC	7 cut(s)	130, 943, 1048, 1653, 2060, 2099, 2374
PmaCI	CACGTG	2 cut(s)	131, 890
PmlI	CACGTG	2 cut(s)	131, 890
PpsI	GAGTC	7 cut(s)	130, 943, 1048, 1653, 2060, 2099, 2374
Ppu21I	YACGTR	2 cut(s)	131, 890
PsiI	TTATAA	2 cut(s)	819, 2181
Psp6I	CCWGG	4 cut(s)	171, 1153, 1329, 1835
PspCI	CACGTG	2 cut(s)	131, 890
PspFI	CCCAGC	3 cut(s)	22, 104, 2251
PspGI	CCWGG	4 cut(s)	171, 1153, 1329, 1835
PspN4I	GGNNCC	4 cut(s)	987, 1159, 1842, 2432
PspPI	GGNCC	3 cut(s)	107, 160, 1158
PstI	CTGCAG	3 cut(s)	1051, 1455, 1503
PsuI	RGATCY	3 cut(s)	529, 985, 2081
PvuII	CAGCTG	1 cut(s)	801
RsaI	GTAC	7 cut(s)	430, 953, 961, 1112, 1328, 1750, 2164
RsaNI	GTAC	7 cut(s)	429, 952, 960, 1111, 1327, 1749, 2163
RseI	CAYNNNNRTG	3 cut(s)	276, 888, 2051
SapI	GCTCTTC	1 cut(s)	2241
SaqAI	TTAA	7 cut(s)	870, 1199, 1560, 1691, 1902, 1937, 1974
Sau96I	GGNCC	3 cut(s)	107, 160, 1158
SbfI	CCTGCAGG	1 cut(s)	1503
SchI	GAGTC	7 cut(s)	130, 944, 1049, 1654, 2060, 2099, 2375
ScrFI	CCNGG	5 cut(s)	173, 1155, 1331, 1837, 2218
SdaI	CCTGCAGG	1 cut(s)	1503
SduI	GDGCHC	3 cut(s)	1843, 2421, 2433
SexAI	ACCWGGT	1 cut(s)	171
SfcI	CTRYAG	5 cut(s)	597, 1047, 1221, 1451, 1499
SmiI	ATTTAAAT	1 cut(s)	1938
SmiMI	CAYNNNNRTG	3 cut(s)	276, 888, 2051
SmlI	CTYRAG	2 cut(s)	1616, 1690
SmoI	CTYRAG	2 cut(s)	1616, 1690
SpeI	ACTAGT	1 cut(s)	425
SphI	GCATGC	4 cut(s)	98, 275, 754, 2101
Sse8387I	CCTGCAGG	1 cut(s)	1503
Sse9I	AATT	8 cut(s)	79, 198, 1003, 1470, 1556, 1933, 1939, 2158
SsiI	CCGC	8 cut(s)	155, 180, 329, 414, 776, 1770, 2200, 2481
SspI	AATATT	2 cut(s)	1978, 2540
SspMI	CTAG	6 cut(s)	426, 525, 831, 939, 1361, 1731
StyD4I	CCNGG	5 cut(s)	171, 1153, 1329, 1835, 2216
StyI	CCWWGG	6 cut(s)	824, 1064, 1145, 1271, 2347, 2360
SwaI	ATTTAAAT	1 cut(s)	1938
TaaI	ACNGT	5 cut(s)	718, 811, 1122, 1755, 2126
TaiI	ACGT	4 cut(s)	133, 892, 1144, 1673
TaqI	TCGA	4 cut(s)	568, 706, 1132, 1295
TasI	AATT	8 cut(s)	79, 198, 1003, 1470, 1556, 1933, 1939, 2158
TatI	WGTACW	2 cut(s)	428, 2162
TauI	GCSGC	4 cut(s)	182, 331, 779, 2202
Tru1I	TTAA	7 cut(s)	870, 1199, 1560, 1691, 1902, 1937, 1974
Tru9I	TTAA	7 cut(s)	870, 1199, 1560, 1691, 1902, 1937, 1974
TscAI	CASTG	5 cut(s)	1125, 1758, 2014, 2131, 2211
TseFI	GTSAC	1 cut(s)	1821
Tsp45I	GTSAC	1 cut(s)	1821
TspDTI	ATGAA	8 cut(s)	17, 54, 306, 681, 1200, 1259, 1644, 1670
TspRI	CASTG	5 cut(s)	1125, 1758, 2014, 2131, 2211
Vha464I	CTTAAG	1 cut(s)	1690
VneI	GTGCAC	1 cut(s)	2417
XapI	RAATTY	4 cut(s)	79, 1003, 1933, 1939
XceI	RCATGY	6 cut(s)	98, 275, 754, 1744, 1789, 2101
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1008
XmiI	GTMKAC	2 cut(s)	147, 1043
XmnI	GAANNNNTTC	1 cut(s)	348
XspI	CTAG	6 cut(s)	426, 525, 831, 939, 1361, 1731
Zsp2I	ATGCAT	3 cut(s)	273, 756, 1173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.