Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004216

Physical Location & Seq

Genomic Coordinates Reverse (-)

36507726 .. 36513577

Gene Structure 5852 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1286400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCGGGCACGGAGGTCAAAGAAGAAGAGCAGCTCCAATTGTTCAGCGTGGGCACAGAAGTAGAAGTGAAGAGCGACGAGGAAGGGTTCAAAGGCGCCTGGTTCAGAGCCACCATTGTCACAAGCCCCACAAATTCCGCCTCAAAGAAGAGGAAGAGGGCGTTGGTCGAGTACAAGAACTTGGTCACGGAAGACGGGTCTAAGCAGCTCAAGGAGTACGTCGATTCGGAGAACCTGAGGCCGACGCCGCCGCAGCTCGCCGACCGGAATTTCGATGTGGGAGATGTTGTGGACGCGGATTACAGAGATGGGTGGTGGACTGGGGTCGTAGGGGAGGCTCTTGACAGCAATTCCAAGTACCGCGTCGTCTTCACGAACCCCCCGGATTTGATCGAATTTCAGAAAGAACGTCTCAGATTGCATCAGGATTGGGTTAATGGCGAATGGGTTCGGCCTAACAAGCAGGATGTATTGGCTGCTTCAGATACTCCACAAGAATTAGAGCATGTGTTGCCTGCTCCAAAAGATCCTAACAATCTTCAAGTGGCTACTCAACTAGAAAATTCGAGTGCTGCAGAGGAGAATCCTGAATCAACTAATTCAGGGAAGAATTTAACAGAGCAACCAAGTTATCCACTAAGTGTCAAGGGCAAAAAGATGTTAGCCCGTAATGGTAGTGCTACAGACTCGCGTCCAGTTAAAAAGTTAAAGGACGAAAAAGCAGCTAAGCCCACAGTATCCGCTACAGCACATCAATTGAGCAAAACGCCCGACAATAAAGAATTGCCCCAGGAATTACCTCAACTGAGCACAGGAGTTAAGGGAACAAGACGCACAAGGAAACCTGTTGTACGCCATCAGTTTTTTAAAACAGAGAGCGTGCTTGGGAAAAATAATGTCAAGACTAAGCAAGAGAATGACGGTGAAGTGAATAGCCAGTGGATTCATCCTGTAGCAAGTAAAGGAAGGCGCACAAAATCTCATGCTGGAAGTCGATTCACCCCAGACGCAGGTAAGGAAGAGTCCGCAAGTGTTTCTTTGGCTGGACAAAATGTTCAGAAGGAGGGTAATATTAAAGAAGCTGAAGGGCCTCAAACTATTAGGTCAACAACTAAGGGCAAGGAGGGTTCACTGGCTGAAATACCGGCTCAGCTACCTGATCAAGAGTTGCAAGTGAAAGATCAGAGGAAGAGTGCAAATGATCCTGCAAAAGAAAAAAGCATGGAGTTTAAGCAGCAACCAGCTGGAGGAAGCAGTCATAAAAGGAAGAGAGGCAGGCCTCGAAAGTTGGTGGTCATAAGATCGCGAGCTTCAGAAGGAGTGAAGGGGCAGAATATTTCAGGAAATGTCGCCAGCAAAAATGTTGCGAATGATCAGACGCCTGAGGAAGCTGCTTTGCGTGTGCTGAGAGGGACGGATCCTACAGATGCACAAGATGCCTCTAAAAGAAAAACGGCTGAACTTCCTGGAAGGTGCATGATAAATGAAGCTTCATTGATAGCATCCGAGAATGCAGATGATGATGACAGACCTCTATCCATGTGGTTTGGAGGGATGCAGCATTCTGCGAGCGTGGGTGAATCAAGACCATCCCCTGATATGAATGTCGACCAGCATAGTGATAGAAAAGGACCAGTCGAGGTAGCAACGGAATCTCCCGCAGTTGCTGCAGTCAGTGGCAGTGGGACAGAGGAGAAGCAAGTGTTTCCATTTGTTAAGAGCTCCCCTGTGTGGAAGACTATTGAAACATTGGAAGTGTTCAAAAAGTTTCCCCAAAATCCTCATTTCCGGCCTTTGATTGAATGCAAAGAGGAGTACCGCGAGGGTTCAGCAATTGGAAACATGATAACCTTTTCCAGTCTGGCGGATAAAATATCCAGGCTGCAGTTCGACGACCATCGAAATGTTTTGGGTAGCATTTTGGAGAGTCTTCTTGACTTGGAGAAGTATGGGTTTAATGTTACAGTTCTACGTGGGCGGGTGAATGATCTGCTGTCTGTTAAAGACAGGCATGGACGGTTTCAGGTTGAGTCAAAAGACGCTGAACACATGATCATGGAGCATTCTCATGAGAAGACCAAACTTGTCGAAGATGCTGAGCATATTGCGAAGAAAATAATCGAGTTGCAGGATAAACATGCATCAATGAAGTCGGAAATGGAGGCCAAAGACCATGAGATTGCTAGATTGAAAATGCATATGGATTCCATGAATGAAGGCATTCAGAGCGCTCGGAGTGATTTTGAGAAGCTAGCTTTGGCTCCCGTGACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046983

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Agenet DUF724

Protein Analysis

758

Amino Acids

84.04

Weight (kDa)

6.72

Isoelectric Point (pI)

45.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Agenet	PF05641	18 - 84	5.5e-20	Agenet domain
DUF724	PF05266	569 - 755	2.3e-64	Protein of unknown function (DUF724)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004216)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g26800 FvH4_1g26800 FvH4_1g26800 FvH4_1g26800
malus_domestica	MD09G1286400.v1.1 MD17G1278800.v1.1
prunus_persica	Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1
pyrus_communis	pycom09g19350 pycom17g27640
rosa_chinensis	RchiOBHm_Chr3g0487021
rosa_laevigata	RLG00000023038
rosa_multiflora	Rmu_co8314393.1_g000001 Rmu_sc0002834.1_g000013 Rmu_sc0005856.1_g000019 Rmu_sc0012853.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395240
rosa_rugosa	Rorug03G0229700 Rorug03G0229800 Rorug03G0229900 Rorug03G0230000 Rorug03G0230100
rosa_samantha	Rh3AG278800 Rh3BG314700 Rh3CG313000 Rh3DG309600
rosa_wichuraiana	Rw3G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1001
AccB1I	GGYRCC	1 cut(s)	95
AccI	GTMKAC	1 cut(s)	1608
AccII	CGCG	5 cut(s)	296, 363, 691, 1306, 1821
AclWI	GGATC	4 cut(s)	521, 1196, 1412, 1425
AcsI	RAATTY	5 cut(s)	133, 268, 395, 562, 610
AcuI	CTGAAG	3 cut(s)	465, 1104, 1296
AcyI	GRCGYC	3 cut(s)	96, 245, 1379
AdeI	CACNNNGTG	1 cut(s)	641
AfaI	GTAC	5 cut(s)	173, 218, 359, 852, 1817
AfeI	AGCGCT	1 cut(s)	2230
AfiI	CCNNNNNNNGG	3 cut(s)	1010, 1247, 1731
AgsI	TTSAA	6 cut(s)	91, 542, 1745, 1762, 1802, 2191
AjnI	CCWGG	4 cut(s)	98, 789, 1465, 1877
AjuI	GAANNNNNNNTTGG	4 cut(s)	146, 178, 2240, 2272
AloI	GAACNNNNNNTCC	2 cut(s)	1038, 1070
Alw21I	GWGCWC	2 cut(s)	812, 1724
Alw26I	GTCTC	1 cut(s)	416
AlwI	GGATC	4 cut(s)	521, 1196, 1412, 1425
AlwNI	CAGNNNCTG	1 cut(s)	1667
Aor51HI	AGCGCT	1 cut(s)	2230
AoxI	GGCC	6 cut(s)	239, 452, 1088, 1277, 1790, 2163
ApoI	RAATTY	5 cut(s)	133, 268, 395, 562, 610
ArsI	GACNNNNNNTTYG	2 cut(s)	254, 286
Asp700I	GAANNNNTTC	2 cut(s)	447, 2220
AspLEI	GCGC	3 cut(s)	98, 972, 2231
AspS9I	GGNCC	2 cut(s)	1088, 1631
AsuC2I	CCSGG	1 cut(s)	383
AsuHPI	GGTGA	4 cut(s)	935, 991, 1589, 1993
AsuNHI	GCTAGC	1 cut(s)	2251
AvaII	GGWCC	1 cut(s)	1631
AxyI	CCTNAGG	2 cut(s)	236, 1383
BaeGI	GKGCMC	2 cut(s)	11, 56
BamHI	GGATCC	1 cut(s)	1417
BanI	GGYRCC	1 cut(s)	95
BanII	GRGCYC	1 cut(s)	1724
BbsI	GAAGAC	5 cut(s)	198, 361, 1742, 1922, 2081
Bbv12I	GWGCWC	2 cut(s)	812, 1724
BccI	CCATC	4 cut(s)	302, 864, 1597, 1905
BceAI	ACGGC	1 cut(s)	1470
BciT130I	CCWGG	4 cut(s)	100, 791, 1467, 1879
BciVI	GTATCC	1 cut(s)	748
BclI	TGATCA	3 cut(s)	1159, 1372, 2052
BcnI	CCSGG	1 cut(s)	383
BcoDI	GTCTC	1 cut(s)	416
BfaI	CTAG	3 cut(s)	557, 2184, 2252
BfmI	CTRYAG	7 cut(s)	573, 681, 744, 951, 1422, 1668, 1883
BfoI	RGCGCY	2 cut(s)	99, 2232
BfuAI	ACCTGC	1 cut(s)	1001
BfuI	GTATCC	1 cut(s)	748
BlpI	GCTNAGC	3 cut(s)	726, 1149, 2097
Bme1390I	CCNGG	5 cut(s)	100, 383, 791, 1467, 1879
Bme18I	GGWCC	1 cut(s)	1631
BmgT120I	GGNCC	2 cut(s)	1088, 1631
BmiI	GGNNCC	3 cut(s)	97, 1419, 2262
BmrFI	CCNGG	5 cut(s)	100, 383, 791, 1467, 1879
BmrI	ACTGGG	1 cut(s)	330
BmsI	GCATC	7 cut(s)	430, 1417, 1426, 1511, 1545, 2083, 2150
BmtI	GCTAGC	1 cut(s)	2255
BmuI	ACTGGG	1 cut(s)	330
BoxI	GACNNNNGTC	2 cut(s)	323, 690
BpiI	GAAGAC	5 cut(s)	198, 361, 1742, 1922, 2081
BpmI	CTGGAG	1 cut(s)	1266
Bpu1102I	GCTNAGC	3 cut(s)	726, 1149, 2097
BpuEI	CTTGAG	1 cut(s)	194
BpuMI	CCSGG	1 cut(s)	383
BsaAI	YACGTR	1 cut(s)	1973
BsaHI	GRCGYC	3 cut(s)	96, 245, 1379
BsaJI	CCNNGG	2 cut(s)	381, 789
BsaWI	WCCGGW	1 cut(s)	264
BsaXI	ACNNNNNCTCC	4 cut(s)	273, 303, 1639, 1669
Bsc4I	CCNNNNNNNGG	3 cut(s)	1010, 1247, 1731
Bse118I	RCCGGY	1 cut(s)	1144
Bse1I	ACTGG	6 cut(s)	325, 695, 937, 1137, 1634, 1857
Bse21I	CCTNAGG	2 cut(s)	236, 1383
BseBI	CCWGG	4 cut(s)	100, 791, 1467, 1879
BseDI	CCNNGG	2 cut(s)	381, 789
BseGI	GGATG	5 cut(s)	472, 946, 1502, 1560, 1589
BseLI	CCNNNNNNNGG	3 cut(s)	1010, 1247, 1731
BseMII	CTCAG	7 cut(s)	227, 427, 797, 1163, 1374, 1397, 2088
BseNI	ACTGG	6 cut(s)	325, 695, 937, 1137, 1634, 1857
BseRI	GAGGAG	3 cut(s)	593, 1706, 1826
BseSI	GKGCMC	2 cut(s)	11, 56
Bsh1236I	CGCG	5 cut(s)	296, 363, 691, 1306, 1821
Bsh1285I	CGRYCG	1 cut(s)	265
BshFI	GGCC	6 cut(s)	241, 454, 1090, 1279, 1792, 2165
BshNI	GGYRCC	1 cut(s)	95
BsiEI	CGRYCG	1 cut(s)	265
BsiHKAI	GWGCWC	2 cut(s)	812, 1724
BsiSI	CCGG	4 cut(s)	265, 383, 1145, 1789
BslFI	GGGAC	2 cut(s)	1426, 1699
BslI	CCNNNNNNNGG	3 cut(s)	1010, 1247, 1731
BsmAI	GTCTC	1 cut(s)	416
BsmBI	CGTCTC	1 cut(s)	416
BsmFI	GGGAC	2 cut(s)	1426, 1699
BsmI	GAATGC	5 cut(s)	1516, 1561, 1808, 2062, 2220
BsnI	GGCC	6 cut(s)	241, 454, 1090, 1279, 1792, 2165
Bsp1286I	GDGCHC	4 cut(s)	11, 56, 812, 1724
Bsp1720I	GCTNAGC	3 cut(s)	726, 1149, 2097
Bsp68I	TCGCGA	1 cut(s)	1306
BspANI	GGCC	6 cut(s)	241, 454, 1090, 1279, 1792, 2165
BspCNI	CTCAG	7 cut(s)	228, 426, 798, 1162, 1375, 1398, 2089
BspFNI	CGCG	5 cut(s)	296, 363, 691, 1306, 1821
BspHI	TCATGA	1 cut(s)	2068
BspLI	GGNNCC	3 cut(s)	97, 1419, 2262
BspMAI	CTGCAG	3 cut(s)	577, 1672, 1887
BspMI	ACCTGC	1 cut(s)	1001
BspOI	GCTAGC	1 cut(s)	2255
BspPI	GGATC	4 cut(s)	521, 1196, 1412, 1425
BspQI	GCTCTTC	2 cut(s)	21, 65
BspT107I	GGYRCC	1 cut(s)	95
BsrFI	RCCGGY	1 cut(s)	1144
BsrI	ACTGG	6 cut(s)	325, 695, 937, 1137, 1634, 1857
BssAI	RCCGGY	1 cut(s)	1144
BssECI	CCNNGG	2 cut(s)	381, 789
BssNI	GRCGYC	3 cut(s)	96, 245, 1379
Bst2UI	CCWGG	4 cut(s)	100, 791, 1467, 1879
Bst4CI	ACNGT	4 cut(s)	736, 923, 1966, 2019
Bst6I	CTCTTC	7 cut(s)	21, 65, 143, 149, 1014, 1184, 1262
BstACI	GRCGYC	3 cut(s)	96, 245, 1379
BstAPI	GCANNNNNTGC	3 cut(s)	511, 1436, 1667
BstBAI	YACGTR	1 cut(s)	1973
BstC8I	GCNNGC	9 cut(s)	7, 258, 516, 881, 1277, 1308, 1354, 1570, 2253
BstF5I	GGATG	5 cut(s)	472, 946, 1502, 1560, 1589
BstFNI	CGCG	5 cut(s)	296, 363, 691, 1306, 1821
BstH2I	RGCGCY	2 cut(s)	99, 2232
BstHHI	GCGC	3 cut(s)	98, 972, 2231
BstMAI	GTCTC	1 cut(s)	416
BstMCI	CGRYCG	1 cut(s)	265
BstMWI	GCNNNNNNNGC	7 cut(s)	247, 253, 460, 511, 1436, 1667, 2226
BstNI	CCWGG	4 cut(s)	100, 791, 1467, 1879
BstNSI	RCATGY	2 cut(s)	509, 2141
BstPAI	GACNNNNGTC	2 cut(s)	323, 690
BstSCI	CCNGG	5 cut(s)	98, 381, 789, 1465, 1877
BstSFI	CTRYAG	7 cut(s)	573, 681, 744, 951, 1422, 1668, 1883
BstSLI	GKGCMC	2 cut(s)	11, 56
BstUI	CGCG	5 cut(s)	296, 363, 691, 1306, 1821
BstV2I	GAAGAC	5 cut(s)	198, 361, 1742, 1922, 2081
BstX2I	RGATCY	2 cut(s)	526, 1417
BstYI	RGATCY	2 cut(s)	526, 1417
Bsu36I	CCTNAGG	2 cut(s)	236, 1383
BsuI	GTATCC	1 cut(s)	748
BsuRI	GGCC	6 cut(s)	241, 454, 1090, 1279, 1792, 2165
BtsCI	GGATG	5 cut(s)	472, 946, 1502, 1560, 1589
BtsI	GCAGTG	1 cut(s)	1687
BtsIMutI	CAGTG	4 cut(s)	944, 1130, 1681, 1687
BtuMI	TCGCGA	1 cut(s)	1306
BveI	ACCTGC	1 cut(s)	1001
Cac8I	GCNNGC	9 cut(s)	7, 258, 516, 881, 1277, 1308, 1354, 1570, 2253
CaiI	CAGNNNCTG	1 cut(s)	1667
CciI	TCATGA	1 cut(s)	2068
CfoI	GCGC	3 cut(s)	98, 972, 2231
Cfr10I	RCCGGY	1 cut(s)	1144
Cfr13I	GGNCC	2 cut(s)	1088, 1631
CseI	GACGC	8 cut(s)	253, 302, 352, 680, 840, 1016, 1387, 2048
Csp6I	GTAC	5 cut(s)	172, 217, 358, 851, 1816
CviQI	GTAC	5 cut(s)	172, 217, 358, 851, 1816
DinI	GGCGCC	1 cut(s)	97
DraI	TTTAAA	1 cut(s)	868
DraIII	CACNNNGTG	1 cut(s)	641
Eam1104I	CTCTTC	7 cut(s)	21, 65, 143, 149, 1014, 1184, 1262
EarI	CTCTTC	7 cut(s)	21, 65, 143, 149, 1014, 1184, 1262
EciI	GGCGGA	2 cut(s)	127, 1880
Ecl136II	GAGCTC	1 cut(s)	1722
Eco147I	AGGCCT	1 cut(s)	1279
Eco24I	GRGCYC	1 cut(s)	1724
Eco47I	GGWCC	1 cut(s)	1631
Eco47III	AGCGCT	1 cut(s)	2230
Eco53kI	GAGCTC	1 cut(s)	1722
Eco57I	CTGAAG	3 cut(s)	465, 1104, 1296
Eco81I	CCTNAGG	2 cut(s)	236, 1383
EcoICRI	GAGCTC	1 cut(s)	1722
EcoO109I	RGGNCCY	1 cut(s)	1088
EcoRII	CCWGG	4 cut(s)	98, 789, 1465, 1877
EcoT22I	ATGCAT	2 cut(s)	2143, 2199
EcoT38I	GRGCYC	1 cut(s)	1724
EgeI	GGCGCC	1 cut(s)	97
EheI	GGCGCC	1 cut(s)	97
Esp3I	CGTCTC	1 cut(s)	416
FalI	AAGNNNNNCTT	2 cut(s)	1021, 1053
FaqI	GGGAC	2 cut(s)	1426, 1699
FauI	CCCGC	2 cut(s)	1666, 1971
FauNDI	CATATG	1 cut(s)	2199
FbaI	TGATCA	3 cut(s)	1159, 1372, 2052
FblI	GTMKAC	1 cut(s)	1608
FokI	GGATG	5 cut(s)	479, 933, 1489, 1567, 1576
FriOI	GRGCYC	1 cut(s)	1724
FspBI	CTAG	3 cut(s)	557, 2184, 2252
GlaI	GCGC	3 cut(s)	97, 971, 2230
GsuI	CTGGAG	1 cut(s)	1266
HaeII	RGCGCY	2 cut(s)	99, 2232
HaeIII	GGCC	6 cut(s)	241, 454, 1090, 1279, 1792, 2165
HapII	CCGG	4 cut(s)	265, 383, 1145, 1789
HgaI	GACGC	8 cut(s)	253, 302, 352, 680, 840, 1016, 1387, 2048
HhaI	GCGC	3 cut(s)	98, 972, 2231
Hin1I	GRCGYC	3 cut(s)	96, 245, 1379
Hin6I	GCGC	3 cut(s)	96, 970, 2229
HinP1I	GCGC	3 cut(s)	96, 970, 2229
HincII	GTYRAC	2 cut(s)	1107, 1609
HindII	GTYRAC	2 cut(s)	1107, 1609
HindIII	AAGCTT	1 cut(s)	1488
HpaII	CCGG	4 cut(s)	265, 383, 1145, 1789
HphI	GGTGA	4 cut(s)	935, 991, 1589, 1993
Hpy166II	GTNNAC	5 cut(s)	292, 318, 1107, 1130, 1609
Hpy8I	GTNNAC	5 cut(s)	292, 318, 1107, 1130, 1609
Hpy99I	CGWCG	5 cut(s)	80, 224, 247, 368, 1895
HpyAV	CCTTC	8 cut(s)	77, 960, 1054, 1079, 1310, 1318, 1464, 2210
HpyCH4III	ACNGT	4 cut(s)	736, 923, 1966, 2019
HpyCH4IV	ACGT	3 cut(s)	219, 409, 1972
HpyF10VI	GCNNNNNNNGC	7 cut(s)	247, 253, 460, 511, 1436, 1667, 2226
HpySE526I	ACGT	3 cut(s)	219, 409, 1972
Hsp92I	GRCGYC	3 cut(s)	96, 245, 1379
HspAI	GCGC	3 cut(s)	96, 970, 2229
KasI	GGCGCC	1 cut(s)	95
Ksp22I	TGATCA	3 cut(s)	1159, 1372, 2052
LguI	GCTCTTC	2 cut(s)	21, 65
LmnI	GCTCC	5 cut(s)	39, 523, 1727, 2059, 2266
LweI	GCATC	7 cut(s)	430, 1417, 1426, 1511, 1545, 2083, 2150
MaeI	CTAG	3 cut(s)	557, 2184, 2252
MaeII	ACGT	3 cut(s)	219, 409, 1972
MaeIII	GTNAC	4 cut(s)	118, 184, 1960, 2266
MfeI	CAATTG	3 cut(s)	38, 755, 1833
MflI	RGATCY	2 cut(s)	526, 1417
MhlI	GDGCHC	4 cut(s)	11, 56, 812, 1724
Mly113I	GGCGCC	1 cut(s)	96
MlyI	GAGTC	4 cut(s)	680, 1031, 1936, 2039
MmeI	TCCRAC	1 cut(s)	2133
Mph1103I	ATGCAT	2 cut(s)	2143, 2199
MroXI	GAANNNNTTC	2 cut(s)	447, 2220
MslI	CAYNNNNRTG	3 cut(s)	1902, 2054, 2067
MspA1I	CMGCKG	1 cut(s)	1244
MspI	CCGG	4 cut(s)	265, 383, 1145, 1789
MspR9I	CCNGG	5 cut(s)	100, 383, 791, 1467, 1879
MunI	CAATTG	3 cut(s)	38, 755, 1833
Mva1269I	GAATGC	5 cut(s)	1516, 1561, 1808, 2062, 2220
MvaI	CCWGG	4 cut(s)	100, 791, 1467, 1879
MvnI	CGCG	5 cut(s)	296, 363, 691, 1306, 1821
MwoI	GCNNNNNNNGC	7 cut(s)	247, 253, 460, 511, 1436, 1667, 2226
NarI	GGCGCC	1 cut(s)	96
NciI	CCSGG	1 cut(s)	383
NdeI	CATATG	1 cut(s)	2199
NheI	GCTAGC	1 cut(s)	2251
NlaIV	GGNNCC	3 cut(s)	97, 1419, 2262
NmuCI	GTSAC	3 cut(s)	118, 184, 2266
NruI	TCGCGA	1 cut(s)	1306
NsiI	ATGCAT	2 cut(s)	2143, 2199
NspI	RCATGY	2 cut(s)	509, 2141
PagI	TCATGA	1 cut(s)	2068
PceI	AGGCCT	1 cut(s)	1279
PciSI	GCTCTTC	2 cut(s)	21, 65
PctI	GAATGC	5 cut(s)	1516, 1561, 1808, 2062, 2220
PdmI	GAANNNNTTC	2 cut(s)	447, 2220
PfeI	GAWTC	8 cut(s)	224, 583, 590, 943, 996, 1580, 1652, 2204
PfoI	TCCNGGA	1 cut(s)	1465
PleI	GAGTC	4 cut(s)	680, 1030, 1935, 2038
PluTI	GGCGCC	1 cut(s)	99
PpsI	GAGTC	4 cut(s)	680, 1030, 1935, 2038
Ppu21I	YACGTR	1 cut(s)	1973
PshAI	GACNNNNGTC	2 cut(s)	323, 690
Psp124BI	GAGCTC	1 cut(s)	1724
Psp6I	CCWGG	4 cut(s)	98, 789, 1465, 1877
PspGI	CCWGG	4 cut(s)	98, 789, 1465, 1877
PspN4I	GGNNCC	3 cut(s)	97, 1419, 2262
PspPI	GGNCC	2 cut(s)	1088, 1631
PstI	CTGCAG	3 cut(s)	577, 1672, 1887
PstNI	CAGNNNCTG	1 cut(s)	1667
PsuI	RGATCY	2 cut(s)	526, 1417
PvuII	CAGCTG	1 cut(s)	1244
RruI	TCGCGA	1 cut(s)	1306
RsaI	GTAC	5 cut(s)	173, 218, 359, 852, 1817
RsaNI	GTAC	5 cut(s)	172, 217, 358, 851, 1816
RseI	CAYNNNNRTG	3 cut(s)	1902, 2054, 2067
SacI	GAGCTC	1 cut(s)	1724
SalI	GTCGAC	1 cut(s)	1607
SapI	GCTCTTC	2 cut(s)	21, 65
Sau96I	GGNCC	2 cut(s)	1088, 1631
SchI	GAGTC	4 cut(s)	680, 1031, 1936, 2039
ScrFI	CCNGG	5 cut(s)	100, 383, 791, 1467, 1879
SduI	GDGCHC	4 cut(s)	11, 56, 812, 1724
SfaNI	GCATC	7 cut(s)	430, 1417, 1426, 1511, 1545, 2083, 2150
SfcI	CTRYAG	7 cut(s)	573, 681, 744, 951, 1422, 1668, 1883
SfoI	GGCGCC	1 cut(s)	97
SinI	GGWCC	1 cut(s)	1631
SmiMI	CAYNNNNRTG	3 cut(s)	1902, 2054, 2067
SmlI	CTYRAG	1 cut(s)	209
SmoI	CTYRAG	1 cut(s)	209
SseBI	AGGCCT	1 cut(s)	1279
SspDI	GGCGCC	1 cut(s)	95
SspI	AATATT	2 cut(s)	1072, 1336
SspMI	CTAG	3 cut(s)	557, 2184, 2252
SstI	GAGCTC	1 cut(s)	1724
StuI	AGGCCT	1 cut(s)	1279
StyD4I	CCNGG	5 cut(s)	98, 381, 789, 1465, 1877
TaaI	ACNGT	4 cut(s)	736, 923, 1966, 2019
TaiI	ACGT	3 cut(s)	222, 412, 1975
TatI	WGTACW	1 cut(s)	171
TauI	GCSGC	2 cut(s)	250, 253
TfiI	GAWTC	8 cut(s)	224, 583, 590, 943, 996, 1580, 1652, 2204
TscAI	CASTG	4 cut(s)	944, 1137, 1681, 1687
TseFI	GTSAC	3 cut(s)	118, 184, 2266
Tsp45I	GTSAC	3 cut(s)	118, 184, 2266
TspDTI	ATGAA	7 cut(s)	935, 1482, 1500, 1616, 2162, 2225, 2229
TspGWI	ACGGA	4 cut(s)	26, 203, 1430, 1664
TspRI	CASTG	4 cut(s)	944, 1137, 1681, 1687
VpaK11BI	GGWCC	1 cut(s)	1631
XapI	RAATTY	5 cut(s)	133, 268, 395, 562, 610
XceI	RCATGY	2 cut(s)	509, 2141
XmiI	GTMKAC	1 cut(s)	1608
XmnI	GAANNNNTTC	2 cut(s)	447, 2220
XspI	CTAG	3 cut(s)	557, 2184, 2252
Zsp2I	ATGCAT	2 cut(s)	2143, 2199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.