Rorug03G0230100

Protein of unknown function (DUF724)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004216
Physical Location & Seq
Forward (+)
21133482 .. 21192548
59067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0230100.1

Sequence Viewer

Length: 3843 bp
ATGGCAGAACTCTTGCAGATTATAACATCCAAAAGGAGTCAACTCTTCACCTGGCCGGTTTTGAGGCTGAGGGGAGGAACTATGATTAAGGAGAAGGCTCTTACATGGAAAGAAATTGAGATTGATATTGAACCGACTGACACCATTGACCAAATGAAGGAAAAGGTTGAGGAGAAAGAGGGAATTCCTCCAGTGCAACAAAGGCTTATCACATGGAATTCAAGTGCTGATTGCTGCTCTTGGCTCGGTGTAACTTGCAGCACTAATGGGTATGTTGTCGGACTTGACCTCAGCAGCGAGTTTGTTTCTTGCAGAATTGACAATTCCAGCAGTCTCTTCCAACTTCAACATCTTGAGAGCCTCAATTTGGCCAATAACGTCTTCAATGGCTCTCAAATTCCATCTACAATTGGAGAGCTTACAAATCTGAGGTATCTAAATTTAGCCAGTGGTTTCTATTCCGGGAAAATTCCCATTGAGATTTCACGCTTGACAAGGTTGGTAAGTCTTGATCTCTCTAGAAATAACTATGATTGGGTTTCGTTAAAACTTGAAAACCCAAATTTAAGCATGCTAATTCAGAACCTCACAGAGCTTACAGAGTTATATCTTGATTCAGTAGAGATATCAGGACAGGGGAGTGACTGGTGCGAAGCCATATCATCTTCACTGCCAAATCTGAGGGTGTTGAGCTTGTCTTTTTGTGGTCTTTCAGGCCCTATTCATGAATCATTTGCCAGGCTTCATTCTCTATCCACGTTAATATTGGATGGAAACAATATCTCTGCTCCAGTTCCAAAATTCTTTGCCAATTTTTCAAGCTTGATTTCCTTGAGTCTCTCTAAAAGTAACTTCCATGGAGCATTTCCCAAAGAGATATTCCAGGTACCTACACTACAGTCTGTTGACCTATCAGATAATTCAGAGCTTCGTGGTTCCTTACCGGAGTTTCCAAAGAATGGATCTCTCAAATCCCTAGTTCTATCCGGGACTAATTTCTCAGGAGTCTTGCCGGACTCTATTGGCAACCTTAAAATGCTGTCTGAATTGGATCTTAGAGAATGCAATTTCACAGGAGCAGTTCCAAAGTCATTGGCCAACCTAACACAATTGGGGCATTTGTACTTGTCATCCAACAAGTTTTCTAGTCCGATTAATTCTATTCAATGGGACAAACTCATCAAGCTTGAGTATCTCGACTTGTCCGACAATCTACTCTACGGGAGTATTCCATTGTCTCCCTTCTCTATTCCATTGCTGCAGAGCCTCAATTTGGCCAATAACTACTTTAATCTCGCTTCAATTCCATCTGCAATTGGAAAGCTTGCAGACTTGAGGTATCTGAATTTAAGTGATGCTTTTTTTTCTGGGACTATTCCTTCATCTGTTGTTTCTCTTCCCAAACTGGAAACATTAACACTATCCCACAATCGGTTCTCGGGTCCAGTCCCTGAATTTTCCAATACCTCTTCGCCCTTACTTGAAACCCTTCATTTGGAAAACAACAATTTAGAAGGACAAATACCAACATCTATCTTCAATCTTCAAGGGCTTCTGTATCTCAATCTTTCTTCAAACAACTTCAGCAGCTTTCCTTTTAATGGTCCTCGGCTGCCCAGAAATCTTTCATCGGTTGACCTTTCCCACAATAGCTTGTTGTTTGATCACAATGACACTAATTCCACCTTCCTTCATATTGAAAATTTGGTATTGGCTTCTAACAAGTTGAGAGCATTCCCAGATTTCTTGAGAACTCAGTCTACATTAGACTACTTGGACCTTTCAAACAACCAGATTCAAGGTGAGATACCTAATTGGATTTTGAGTCTCAAATCTCTTAATTCCCTTGATCTTTCTGGCAACTCCTTGGTGACTCTAGAAGCTCCTCTTCCTAATTTTACTTCTGCTTTATCTTCTCTGAACCTTCATTCCAACAAGCTTCAAGGCGAGATACCTAATTGGATTTTGAGTCTTAAATCTCTTAATTCCCTTGATCTTTCTGGCAACTCCTTGGTGACTCTAGAAGCTCCTCTTCCTAATTCCACTTCTACTTTATCTTCTCTGGACATTCATTCTAACAAGCTTCATGGTGAGATACCTAATTGGATTTTGAGTCTCAAAACTCTTAATTCCCTTGATCTTTCTAACAACTCCTTGGTGACTCTAGAAGCTCCTCTACCTAATTCCACTTCTGCTTTATCTTCTCTGAACCTTCATTCCAACAAGCTTCAAGAAGCTCCTCTTCCTAATTCCACTTCTGCTTTATCTTCTTTGGACCTTCATTCCAACAAGCTTCAAGTTGATCTTCATTCAAACCAGCTTCAGGGACATTTCCCCTTGTTCTTAAATAGTAGTTATCTAGATTACTCAAGAAATAATTTCAACTCTAGTATATTGATTGACATTGGTAATTTCATTTCTACTGGGTCTTACCTTTCTCTTTCAAGTAATAACTTTCACGGGGCCATTCCAAAATCAATATGCAATGCAAGTGGTGTGGTTCTTGATCTGTCCAATAATTCCTTGAGTGGAAACATTCCCCAATGCTTGACTCAATCACATTCGCTTTTGGCCATTGATATGAGGAGAAACAACCTGAGTGGCACATTTCCTGATAATTTTCTCAAGGATTGTGAGCTACGAACTCTCGACCTCAGCAACAATCAAAGACAAGGCCAACTTCCGAAATCTCTTGTCAACTGCTCAAAGTTAGAGGTTTTAAACCTTGGAAACAATAACATAACTGATACTTTTCCATGCTTTTTGATGAGCATATCCACCTTGCGTGTCCTTGTTTTGCGGTCCAACAAGTTTTATGGAAGCATTAGATGTCCCAAGACAAATGGCACATGGCCTGTGCTTCAAATAATAGATTTAGCTTACAACAATTTCAGTGGTGATATGCCAGGAAGAATGTCTCTGACTTGGCAAGCTATGGTGTCTAATGAGGATGATTCTCCAACTAACCTTGGATTTTCTTATAAGCTGCCGGGCAGGTATGATAGAGATAATGAAAATATATCTACTAGAGACAATATTCAGTTGGTTGCTCGGGGCGATTATTATGGAGATGCATCTATGGCTCGAAATAATCTGCCATGCAGGTGTAAGACAGATAATAAAAATCCAGTTGCAAGATATAATTATCGGTATGTTACAGGTCCTAGCGTTTATTATAGAAATGCTATTACGACTATCATCAAAGGTTTAGAGTTGAATGTGGTTAAGATTCTAACTATATTCACCTTGATTGACTACTCCTCCAACAAATTCAACGGATCAATACCTGAGGAAATCGGAGAGTTGAAATTACTGTATGTCCTCAACTTATCCGGTAATGCTTTTACAGGAAAAATCCCATCATCATTTGGTAAGATGCAACAACTGGAGTCCTTAGATCTCTCAAAGAACCACTTGAGCGGCCAAATTCCCCCACAGTTTGTATATCTCACTTTCCTTGCATTCTTGGATCTCTCAAATAATGAACTGGTCGGGAAGATCCCAACCAGCACTCAAATTTCAACATTTCCAAATACCTCATTCGAAGGTAACAAAGGATTATGGGAGCCTCCTTTGACAAAGGATGCAGTATTGCCGCCACCAATTTTAAATGGAAGCTCAAGTAATCCTAATTCTGGAGATGAGATTGATTGGGATGTTATCTGTGTTGAAATTGGATTTACATGTGGGTTTGGAATTGTCATCGGGTCACTTTTGTTTTGCAAAAGATGGAGGAAATGGTATTACAGAGCTATACGTAGTATTCTTTTCAAGATATTCCCTCAGCTGGAGCAAAGATTTGGTAATCATAGAAGACACGTCTACGTAAACACAAGGCGTTGA

Protein Analysis

1280

Amino Acids

141.89

Weight (kDa)

5.98

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 34 - 70 8e-08 Ubiquitin family
LRRNT_2 PF08263 66 - 87 4.2e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 108 - 303 1.7e-10 Leucine-rich repeat region
LRR_14 PF23598 318 - 401 1.7e-07 Leucine-rich repeat region
LRR_8 PF13855 322 - 381 7.4e-07 Leucine rich repeat
LRR_8 PF13855 347 - 406 4.8e-06 Leucine rich repeat
LRR_8 PF13855 419 - 479 8e-06 Leucine rich repeat
LRR_8 PF13855 566 - 623 4.3e-07 Leucine rich repeat
LRR_8 PF13855 879 - 938 2.3e-06 Leucine rich repeat
LRR_8 PF13855 1105 - 1163 1.5e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 23, 2982
AarI CACCTGC 1 cut(s) 3093
Acc36I ACCTGC 2 cut(s) 2985, 3093
Acc65I GGTACC 1 cut(s) 886
AccB1I GGYRCC 1 cut(s) 886
AccB7I CCANNNNNTGG 1 cut(s) 959
AccBSI CCGCTC 1 cut(s) 3420
AccI GTMKAC 2 cut(s) 1760, 3822
AciI CCGC 3 cut(s) 2800, 3420, 3596
AclWI GGATC 5 cut(s) 970, 1059, 3286, 3477, 3493
AcoI YGGCCR 6 cut(s) 53, 369, 1095, 1275, 2571, 3421
AcuI CTGAAG 2 cut(s) 1567, 2306
AfaI GTAC 2 cut(s) 888, 1124
AflIII ACRYGT 2 cut(s) 3683, 3817
AjiI CACGTC 1 cut(s) 3820
AjnI CCWGG 4 cut(s) 50, 737, 882, 2905
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 863, 895
AloI GAACNNNNNNTCC 4 cut(s) 1913, 1945, 2201, 2233
Alw26I GTCTC 7 cut(s) 338, 842, 1242, 1832, 2120, 2922, 3024
AlwI GGATC 5 cut(s) 970, 1059, 3286, 3477, 3493
AlwNI CAGNNNCTG 1 cut(s) 1451
Ama87I CYCGRG 2 cut(s) 1438, 3051
ApeKI GCWGC 7 cut(s) 234, 258, 294, 1258, 1587, 1612, 2986
AseI ATTAAT 1 cut(s) 1155
Asp700I GAANNNNTTC 2 cut(s) 1488, 1624
Asp718I GGTACC 1 cut(s) 886
AspS9I GGNCC 8 cut(s) 716, 1442, 1604, 1777, 2275, 2463, 2802, 3161
AsuC2I CCSGG 3 cut(s) 463, 988, 2991
AsuHPI GGTGA 8 cut(s) 40, 1814, 1882, 2026, 2102, 2170, 2909, 3235
AsuII TTCGAA 1 cut(s) 3543
AvaI CYCGRG 2 cut(s) 1438, 3051
AvaII GGWCC 6 cut(s) 1442, 1604, 1777, 2275, 2802, 3161
AxyI CCTNAGG 1 cut(s) 3288
BalI TGGCCA 4 cut(s) 371, 1097, 1277, 2573
BanI GGYRCC 1 cut(s) 886
BarI GAAGNNNNNNTAC 4 cut(s) 1506, 1538, 2160, 2192
BbsI GAAGAC 2 cut(s) 373, 3820
BbvCI CCTCAGC 4 cut(s) 68, 290, 2654, 3783
BbvI GCAGC 7 cut(s) 221, 270, 306, 1245, 1599, 1599, 2973
BccI CCATC 5 cut(s) 409, 764, 1315, 3367, 3723
BciT130I CCWGG 4 cut(s) 52, 739, 884, 2907
BclI TGATCA 1 cut(s) 1663
BcnI CCSGG 3 cut(s) 463, 988, 2991
BcoDI GTCTC 7 cut(s) 338, 842, 1242, 1832, 2120, 2922, 3024
BfmI CTRYAG 2 cut(s) 896, 1259
BfuAI ACCTGC 2 cut(s) 2985, 3093
BglII AGATCT 1 cut(s) 3397
BisI GCNGC 9 cut(s) 235, 259, 295, 1259, 1588, 1613, 2987, 3421, 3596
BlsI GCNGC 9 cut(s) 236, 260, 296, 1260, 1589, 1614, 2988, 3422, 3597
Bme1390I CCNGG 7 cut(s) 52, 463, 739, 884, 988, 2907, 2991
Bme18I GGWCC 6 cut(s) 1442, 1604, 1777, 2275, 2802, 3161
BmeT110I CYCGRG 2 cut(s) 1438, 3051
BmgBI CACGTC 1 cut(s) 3820
BmgT120I GGNCC 8 cut(s) 716, 1442, 1604, 1777, 2275, 2463, 2802, 3161
BmiI GGNNCC 5 cut(s) 888, 937, 1443, 2464, 3567
BmrFI CCNGG 7 cut(s) 52, 463, 739, 884, 988, 2907, 2991
BmrI ACTGGG 1 cut(s) 2433
BmsI GCATC 5 cut(s) 1345, 3061, 3083, 3366, 3574
BmuI ACTGGG 1 cut(s) 2433
BpiI GAAGAC 2 cut(s) 373, 3820
BpmI CTGGAG 5 cut(s) 174, 774, 3407, 3657, 3809
Bpu10I CCTNAGC 4 cut(s) 68, 290, 2654, 3783
Bpu14I TTCGAA 1 cut(s) 3543
BpuMI CCSGG 3 cut(s) 463, 988, 2991
BsaAI YACGTR 2 cut(s) 3758, 3826
BsaJI CCNNGG 7 cut(s) 856, 1607, 1866, 2010, 2154, 2725, 2968
BsaWI WCCGGW 2 cut(s) 943, 3332
BsaXI ACNNNNNCTCC 2 cut(s) 3245, 3275
Bse118I RCCGGY 1 cut(s) 55
Bse21I CCTNAGG 1 cut(s) 3288
Bse3DI GCAATG 2 cut(s) 1253, 2491
BseBI CCWGG 4 cut(s) 52, 739, 884, 2907
BseDI CCNNGG 7 cut(s) 856, 1607, 1866, 2010, 2154, 2725, 2968
BseGI GGATG 6 cut(s) 26, 775, 1130, 2956, 3589, 3661
BseMI GCAATG 2 cut(s) 1253, 2491
BseRI GAGGAG 7 cut(s) 185, 1875, 2019, 2163, 2229, 2599, 3250
BseXI GCAGC 7 cut(s) 221, 270, 306, 1245, 1599, 1599, 2973
BshNI GGYRCC 1 cut(s) 886
BsiHKCI CYCGRG 2 cut(s) 1438, 3051
BsiSI CCGG 7 cut(s) 56, 462, 944, 987, 1013, 2990, 3333
BslFI GGGAC 6 cut(s) 1003, 1184, 1384, 1433, 2340, 2817
BsmAI GTCTC 7 cut(s) 338, 842, 1242, 1832, 2120, 2922, 3024
BsmFI GGGAC 6 cut(s) 1003, 1184, 1384, 1433, 2340, 2817
BsmI GAATGC 3 cut(s) 1067, 1733, 3461
BsoBI CYCGRG 2 cut(s) 1438, 3051
Bsp119I TTCGAA 1 cut(s) 3543
Bsp19I CCATGG 1 cut(s) 856
BspACI CCGC 3 cut(s) 2800, 3420, 3596
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 5 cut(s) 888, 937, 1443, 2464, 3567
BspMAI CTGCAG 1 cut(s) 1263
BspMI ACCTGC 2 cut(s) 2985, 3093
BspPI GGATC 5 cut(s) 970, 1059, 3286, 3477, 3493
BspT104I TTCGAA 1 cut(s) 3543
BspT107I GGYRCC 1 cut(s) 886
BsrBI CCGCTC 1 cut(s) 3420
BsrDI GCAATG 2 cut(s) 1253, 2491
BsrFI RCCGGY 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 7 cut(s) 856, 1607, 1866, 2010, 2154, 2725, 2968
BssT1I CCWWGG 6 cut(s) 856, 1866, 2010, 2154, 2725, 2968
Bst2UI CCWGG 4 cut(s) 52, 739, 884, 2907
Bst4CI ACNGT 3 cut(s) 900, 3315, 3438
Bst6I CTCTTC 7 cut(s) 50, 341, 1401, 1474, 1893, 2037, 2247
BstBAI YACGTR 2 cut(s) 3758, 3826
BstBI TTCGAA 1 cut(s) 3543
BstC8I GCNNGC 3 cut(s) 572, 1326, 2931
BstDSI CCRYGG 1 cut(s) 856
BstF5I GGATG 6 cut(s) 26, 775, 1130, 2956, 3589, 3661
BstMAI GTCTC 7 cut(s) 338, 842, 1242, 1832, 2120, 2922, 3024
BstMWI GCNNNNNNNGC 2 cut(s) 202, 3080
BstNI CCWGG 4 cut(s) 52, 739, 884, 2907
BstNSI RCATGY 2 cut(s) 574, 3687
BstSCI CCNGG 7 cut(s) 50, 461, 737, 882, 986, 2905, 2989
BstSFI CTRYAG 2 cut(s) 896, 1259
BstSNI TACGTA 2 cut(s) 3758, 3826
BstV1I GCAGC 7 cut(s) 221, 270, 306, 1245, 1599, 1599, 2973
BstV2I GAAGAC 2 cut(s) 373, 3820
BstX2I RGATCY 5 cut(s) 962, 1051, 3397, 3469, 3498
BstYI RGATCY 5 cut(s) 962, 1051, 3397, 3469, 3498
Bsu36I CCTNAGG 1 cut(s) 3288
BtgI CCRYGG 1 cut(s) 856
BtrI CACGTC 1 cut(s) 3820
BtsCI GGATG 6 cut(s) 26, 775, 1130, 2956, 3589, 3661
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 4 cut(s) 198, 454, 668, 2899
BveI ACCTGC 2 cut(s) 2985, 3093
Cac8I GCNNGC 3 cut(s) 572, 1326, 2931
CaiI CAGNNNCTG 1 cut(s) 1451
CciI TCATGA 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 8 cut(s) 716, 1442, 1604, 1777, 2275, 2463, 2802, 3161
Csp6I GTAC 2 cut(s) 887, 1123
CspCI CAANNNNNGTGG 8 cut(s) 2473, 2478, 2508, 2513, 2581, 2616, 2875, 2910
CviQI GTAC 2 cut(s) 887, 1123
DraI TTTAAA 2 cut(s) 2721, 3609
EaeI YGGCCR 6 cut(s) 53, 369, 1095, 1275, 2571, 3421
Eam1104I CTCTTC 7 cut(s) 50, 341, 1401, 1474, 1893, 2037, 2247
EarI CTCTTC 7 cut(s) 50, 341, 1401, 1474, 1893, 2037, 2247
Eco105I TACGTA 2 cut(s) 3758, 3826
Eco130I CCWWGG 6 cut(s) 856, 1866, 2010, 2154, 2725, 2968
Eco32I GATATC 1 cut(s) 627
Eco47I GGWCC 6 cut(s) 1442, 1604, 1777, 2275, 2802, 3161
Eco57I CTGAAG 2 cut(s) 1567, 2306
Eco81I CCTNAGG 1 cut(s) 3288
Eco88I CYCGRG 2 cut(s) 1438, 3051
EcoO109I RGGNCCY 2 cut(s) 716, 3161
EcoRI GAATTC 2 cut(s) 183, 217
EcoRII CCWGG 4 cut(s) 50, 737, 882, 2905
EcoRV GATATC 1 cut(s) 627
EcoT14I CCWWGG 6 cut(s) 856, 1866, 2010, 2154, 2725, 2968
EcoT22I ATGCAT 1 cut(s) 3076
ErhI CCWWGG 6 cut(s) 856, 1866, 2010, 2154, 2725, 2968
FaqI GGGAC 6 cut(s) 1003, 1184, 1384, 1433, 2340, 2817
FbaI TGATCA 1 cut(s) 1663
FblI GTMKAC 2 cut(s) 1760, 3822
Fnu4HI GCNGC 9 cut(s) 235, 259, 295, 1259, 1588, 1613, 2987, 3421, 3596
FokI GGATG 6 cut(s) 13, 782, 1117, 2963, 3596, 3668
Fsp4HI GCNGC 9 cut(s) 235, 259, 295, 1259, 1588, 1613, 2987, 3421, 3596
GluI GCNGC 9 cut(s) 235, 259, 295, 1259, 1588, 1613, 2987, 3421, 3596
GsuI CTGGAG 5 cut(s) 174, 774, 3407, 3657, 3809
HapII CCGG 7 cut(s) 56, 462, 944, 987, 1013, 2990, 3333
HincII GTYRAC 4 cut(s) 41, 907, 1636, 2698
HindII GTYRAC 4 cut(s) 41, 907, 1636, 2698
HindIII AAGCTT 7 cut(s) 820, 1184, 1322, 1937, 2081, 2225, 2291
HpaII CCGG 7 cut(s) 56, 462, 944, 987, 1013, 2990, 3333
HphI GGTGA 8 cut(s) 40, 1814, 1882, 2026, 2102, 2170, 2909, 3235
Hpy166II GTNNAC 7 cut(s) 41, 907, 1636, 1761, 2698, 3823, 3829
Hpy8I GTNNAC 7 cut(s) 41, 907, 1636, 1761, 2698, 3823, 3829
HpyCH4III ACNGT 3 cut(s) 900, 3315, 3438
HpyCH4IV ACGT 5 cut(s) 378, 758, 3757, 3819, 3825
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 3080
HpySE526I ACGT 5 cut(s) 378, 758, 3757, 3819, 3825
KpnI GGTACC 1 cut(s) 890
Ksp22I TGATCA 1 cut(s) 1663
LmnI GCTCC 9 cut(s) 793, 860, 1076, 1888, 2032, 2176, 2242, 3565, 3790
Lsp1109I GCAGC 7 cut(s) 221, 270, 306, 1245, 1599, 1599, 2973
LweI GCATC 5 cut(s) 1345, 3061, 3083, 3366, 3574
MaeII ACGT 5 cut(s) 378, 758, 3757, 3819, 3825
MaeIII GTNAC 9 cut(s) 250, 641, 848, 1870, 2014, 2158, 3154, 3548, 3708
MbiI CCGCTC 1 cut(s) 3420
MfeI CAATTG 3 cut(s) 408, 1109, 1314
MflI RGATCY 5 cut(s) 962, 1051, 3397, 3469, 3498
MlsI TGGCCA 4 cut(s) 371, 1097, 1277, 2573
MluNI TGGCCA 4 cut(s) 371, 1097, 1277, 2573
Mox20I TGGCCA 4 cut(s) 371, 1097, 1277, 2573
Mph1103I ATGCAT 1 cut(s) 3076
MroXI GAANNNNTTC 2 cut(s) 1488, 1624
MscI TGGCCA 4 cut(s) 371, 1097, 1277, 2573
MslI CAYNNNNRTG 2 cut(s) 2579, 3103
Msp20I TGGCCA 4 cut(s) 371, 1097, 1277, 2573
MspA1I CMGCKG 1 cut(s) 3787
MspI CCGG 7 cut(s) 56, 462, 944, 987, 1013, 2990, 3333
MspR9I CCNGG 7 cut(s) 52, 463, 739, 884, 988, 2907, 2991
MunI CAATTG 3 cut(s) 408, 1109, 1314
Mva1269I GAATGC 3 cut(s) 1067, 1733, 3461
MvaI CCWGG 4 cut(s) 52, 739, 884, 2907
MwoI GCNNNNNNNGC 2 cut(s) 202, 3080
NciI CCSGG 3 cut(s) 463, 988, 2991
NcoI CCATGG 1 cut(s) 856
NlaIV GGNNCC 5 cut(s) 888, 937, 1443, 2464, 3567
NmeAIII GCCGAG 1 cut(s) 1588
NmuCI GTSAC 5 cut(s) 641, 1870, 2014, 2158, 3708
NsiI ATGCAT 1 cut(s) 3076
NspI RCATGY 2 cut(s) 574, 3687
NspV TTCGAA 1 cut(s) 3543
PaeI GCATGC 1 cut(s) 574
PagI TCATGA 1 cut(s) 724
PaqCI CACCTGC 1 cut(s) 3093
PciI ACATGT 1 cut(s) 3683
PcsI WCGNNNNNNNCGW 1 cut(s) 1203
PctI GAATGC 3 cut(s) 1067, 1733, 3461
PdmI GAANNNNTTC 2 cut(s) 1488, 1624
PfeI GAWTC 5 cut(s) 614, 728, 1795, 2954, 3229
PflMI CCANNNNNTGG 1 cut(s) 959
PfoI TCCNGGA 2 cut(s) 461, 986
PkrI GCNGC 9 cut(s) 236, 260, 296, 1260, 1589, 1614, 2988, 3422, 3597
Ppu21I YACGTR 2 cut(s) 3758, 3826
PpuMI RGGWCCY 1 cut(s) 3161
PscI ACATGT 1 cut(s) 3683
PshBI ATTAAT 1 cut(s) 1155
PsiI TTATAA 2 cut(s) 23, 2982
Psp5II RGGWCCY 1 cut(s) 3161
Psp6I CCWGG 4 cut(s) 50, 737, 882, 2905
PspGI CCWGG 4 cut(s) 50, 737, 882, 2905
PspN4I GGNNCC 5 cut(s) 888, 937, 1443, 2464, 3567
PspPI GGNCC 8 cut(s) 716, 1442, 1604, 1777, 2275, 2463, 2802, 3161
PspPPI RGGWCCY 1 cut(s) 3161
PsrI GAACNNNNNNTAC 2 cut(s) 1744, 1776
PstI CTGCAG 1 cut(s) 1263
PstNI CAGNNNCTG 1 cut(s) 1451
PsuI RGATCY 5 cut(s) 962, 1051, 3397, 3469, 3498
PvuII CAGCTG 1 cut(s) 3787
RsaI GTAC 2 cut(s) 888, 1124
RsaNI GTAC 2 cut(s) 887, 1123
RseI CAYNNNNRTG 2 cut(s) 2579, 3103
SatI GCNGC 9 cut(s) 235, 259, 295, 1259, 1588, 1613, 2987, 3421, 3596
Sau96I GGNCC 8 cut(s) 716, 1442, 1604, 1777, 2275, 2463, 2802, 3161
ScrFI CCNGG 7 cut(s) 52, 463, 739, 884, 988, 2907, 2991
SfaNI GCATC 5 cut(s) 1345, 3061, 3083, 3366, 3574
SfcI CTRYAG 2 cut(s) 896, 1259
SfuI TTCGAA 1 cut(s) 3543
SinI GGWCC 6 cut(s) 1442, 1604, 1777, 2275, 2802, 3161
SmiMI CAYNNNNRTG 2 cut(s) 2579, 3103
SnaBI TACGTA 2 cut(s) 3758, 3826
SphI GCATGC 1 cut(s) 574
SsiI CCGC 3 cut(s) 2800, 3420, 3596
SspI AATATT 2 cut(s) 765, 3037
StyD4I CCNGG 7 cut(s) 50, 461, 737, 882, 986, 2905, 2989
StyI CCWWGG 6 cut(s) 856, 1866, 2010, 2154, 2725, 2968
TaaI ACNGT 3 cut(s) 900, 3315, 3438
TaiI ACGT 5 cut(s) 381, 761, 3760, 3822, 3828
TaqI TCGA 4 cut(s) 1197, 2649, 3085, 3543
TatI WGTACW 1 cut(s) 1122
TauI GCSGC 2 cut(s) 3423, 3598
TfiI GAWTC 5 cut(s) 614, 728, 1795, 2954, 3229
TscAI CASTG 4 cut(s) 198, 454, 675, 2899
TseFI GTSAC 5 cut(s) 641, 1870, 2014, 2158, 3708
TseI GCWGC 7 cut(s) 234, 258, 294, 1258, 1587, 1612, 2986
Tsp45I GTSAC 5 cut(s) 641, 1870, 2014, 2158, 3708
TspGWI ACGGA 1 cut(s) 3291
TspRI CASTG 4 cut(s) 198, 454, 675, 2899
Van91I CCANNNNNTGG 1 cut(s) 959
VpaK11BI GGWCC 6 cut(s) 1442, 1604, 1777, 2275, 2802, 3161
VspI ATTAAT 1 cut(s) 1155
XbaI TCTAGA 5 cut(s) 518, 1876, 2020, 2164, 2359
XceI RCATGY 2 cut(s) 574, 3687
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XmiI GTMKAC 2 cut(s) 1760, 3822
XmnI GAANNNNTTC 2 cut(s) 1488, 1624
Zsp2I ATGCAT 1 cut(s) 3076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.