Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0004216

Physical Location & Seq

Genomic Coordinates Reverse (-)

16311218 .. 16317712

Gene Structure 6495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00395240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGGCGGGGTTCGATGGCTCCGAATCCCAGCAACAAAACCAGCTATTCAGCGTAGGGTCAGAAGTGGAAGTGAGGAGCGACGAGGACGGGTTCAAAGGAGCGTGGTTCAGAGCCACCATTGTTCAGAGCCCAGCTGCTAGCTCAGCCTCAAAGAAGAAGAAGAAGGCTCTGGTGGAGTACAAGACCTTGGTCACCGAAGACGGGTCTCAGCAGCCACTGAAAGAACACGTCGATTTCGGTTATATAAGGCCTCTGCCGCCTCACGTGCCTGACCAAGATCTTGAATACGGCCACATAGTCGACGCCGACTACAAAGACGGCTGGTGGACCGGAGTTATTGACAAGGTTATCAAGAAGAACTCCAAATATAGGATCTTCTTTGAGAACCCGCCCGACTTTCTGGAATTCGAGAGAGACCGTCTGCGACTCCATCAGGATTGGGTTGCCGGCTTTTGGGTTCGCCCCGGCAAGCAGGAGATACTGAGTGCTTCAGATTCTCAACAAGCGGAAGAGCATCGGCTGCCGCCTGTCCAAAATGATTCCAACCATCCTGAGGTGGCTGCACAACCGAAAACTTCAGATGCTGCAGGGGATAACCCTGAATCAACTAGTTCAAGGAAGAATCTTGTGGAGCAGTCAAGTTTTACCCAAAGCATCAAGGATGTAGCACCTAATGGTGGTGCTACAGACTTGCGTCCTTTGAAAAAATTAAAGGACGACAAAGTGTCAGAGGCTGCATCATCTGTTACACCACGGCAATTGAGGAAAAATCCAAATGATAAAGAAATGCTATTTGAATTAGACACTGCGACCAGGGAAAGCAGTGGAACTAGAGGATCAAGTCGAGTAAGAAAACCGGCTGTTAATCCTCAGCTTTTTCAAGTAGAGAGCTTGGTTGGTGAAAAGAAAGTTAAGACAAAGCAAGAGAAAGACGGTGAGCTAGAAAACCTCTGCACACACTCTGTCAAAAGTAAAGGAAGGCGTTCGAAGGCTCGAGTTGGAAGTGCCCCAGTTATATGTGCACTTCCTAGCCAAGAGAGACATAAGGAAAAGGGTAAGGAAGGGTATGGAGTTATTGGTTCTGCTGGAAATGTTGTTCAAAAGGAGGGCATAAGTAAAGAAGCTGAATCGTCTCTCACTATAGAGTCAAAGGTCAATGGAAAGCAGGATTCATCAGCTGAATATCCATGTCAGATTCCCAACGAAGATTTGCTGAAGCTCGTGAGAGATCAGAAGAAGAATGCAAATGGTCCAGCACAAGAAAACAGCACGGTTACGTCAGCTGAACATGGCAGTGATCCAGTTTTGGATAACCTTCTCCGTAGCCTGGTTCTTTTTCCAGACATGGATTTTAAGCAGCAACCAACTGAAGGAAGCAGCCACAAAAGAAAGAGGGGCAGACCTCCTAAAAAGGTTCCTAAAAACTCTATCGGAATCACAGAAGCCTCACAGGGAGTCAGGGAACTAAATGGATCAAGAAATGTTGCTGACAAAAGTGCTGTAGAAGATCAGAGACTTGATGAAGTTGCTTTGCATTTTCCTGGAGGGAGAGATGCTTCAGATTCACAAGACGCGTCCAGGAGAAATACAGCTGATGTTCCTGGAACAAGGGGCATACCAAATGAAGCTGCTAGGACCCGTGTTCAAGCATCGGAAAATATAGATGATGATGACCAACCTCTCTCCACATGGGTTGTAGGAGTGCAGGGTCCAGCAAACATGGATGAATTAAGTAAAACTGTTGAACAAGTTAACCTTGCCAGAGATTCTACTCCAGTTGATTCAGTCAGTGACACTGTGCCAGATGAGAACCAAATTATGCCATTTGTGAAGAGCTCCCCAATCTGGAAAGCAATTGAATCATTTGACGTATTCAGAATAATGCCACAAAATCCACATTTTCGTCCCTTGGTTAAATGTAAAGAGGAATATCGCGAGGGATTTGCTATTGGCAATATGGTGACCTTTTCCAGTTTGGTGGAGAAGATATCGAGTCTGCATTTTGATGACCCTATACAGGTTTTTGATAGCATTTTGGAGAGTCTTTTTGACTTGGAAAAATATGGATTCAATGTTACCGTTTTACGTGGGCGTGTGAACGAACTTTTGTCTATCAAACAAAGGCAAGGACAGTTTGAGGGCGTATCAAAAGACGCGGAAAGTAAGATAGTGGAGCAAACTCGAGAGAAATCAAAACTTGAAGATGAGGCTGATTGCATCGAGAAAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046983

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Agenet DUF724

Protein Analysis

743

Amino Acids

82.5

Weight (kDa)

5.76

Isoelectric Point (pI)

42.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Agenet	PF05641	19 - 87	1.6e-18	Agenet domain
DUF724	PF05266	607 - 743	6.6e-50	Protein of unknown function (DUF724)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004216)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g26800 FvH4_1g26800 FvH4_1g26800 FvH4_1g26800
malus_domestica	MD09G1286400.v1.1 MD17G1278800.v1.1
prunus_persica	Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1
pyrus_communis	pycom09g19350 pycom17g27640
rosa_chinensis	RchiOBHm_Chr3g0487021
rosa_laevigata	RLG00000023038
rosa_multiflora	Rmu_co8314393.1_g000001 Rmu_sc0002834.1_g000013 Rmu_sc0005856.1_g000019 Rmu_sc0012853.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395240
rosa_rugosa	Rorug03G0229700 Rorug03G0229800 Rorug03G0229900 Rorug03G0230000 Rorug03G0230100
rosa_samantha	Rh3AG278800 Rh3BG314700 Rh3CG313000 Rh3DG309600
rosa_wichuraiana	Rw3G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	300
AccII	CGCG	3 cut(s)	1574, 1935, 2156
AciI	CCGC	6 cut(s)	5, 257, 389, 506, 524, 2156
AclWI	GGATC	4 cut(s)	380, 844, 1292, 1480
AcoI	YGGCCR	1 cut(s)	289
AcsI	RAATTY	1 cut(s)	404
AcuI	CTGAAG	5 cut(s)	474, 561, 1235, 1389, 1542
AcvI	CACGTG	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	1 cut(s)	179
AfiI	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 553, 677, 1327, 1370, 1845, 2017
AflIII	ACRYGT	2 cut(s)	226, 1572
AhdI	GACNNNNNGTC	1 cut(s)	724
AhlI	ACTAGT	1 cut(s)	608
AjiI	CACGTC	1 cut(s)	229
AjnI	CCWGG	5 cut(s)	812, 1326, 1540, 1577, 1600
AloI	GAACNNNNNNTCC	2 cut(s)	1080, 1112
Alw21I	GWGCWC	2 cut(s)	1024, 1838
Alw26I	GTCTC	5 cut(s)	210, 408, 1033, 1137, 1507
Alw44I	GTGCAC	1 cut(s)	1020
AlwI	GGATC	4 cut(s)	380, 844, 1292, 1480
AlwNI	CAGNNNCTG	3 cut(s)	217, 584, 734
Ama87I	CYCGRG	2 cut(s)	993, 2181
AoxI	GGCC	2 cut(s)	248, 289
ApaLI	GTGCAC	1 cut(s)	1020
ApeKI	GCWGC	9 cut(s)	134, 211, 520, 560, 584, 734, 1357, 1377, 1628
ApoI	RAATTY	1 cut(s)	404
Asp700I	GAANNNNTTC	1 cut(s)	982
AspS9I	GGNCC	4 cut(s)	327, 1250, 1635, 1709
AsuC2I	CCSGG	1 cut(s)	465
AsuHPI	GGTGA	4 cut(s)	184, 911, 947, 1972
AsuII	TTCGAA	1 cut(s)	986
AsuNHI	GCTAGC	1 cut(s)	137
AvaI	CYCGRG	2 cut(s)	993, 2181
AvaII	GGWCC	4 cut(s)	327, 1250, 1635, 1709
AxyI	CCTNAGG	1 cut(s)	552
BaeGI	GKGCMC	2 cut(s)	1009, 1024
BanII	GRGCYC	2 cut(s)	131, 1838
BauI	CACGAG	1 cut(s)	1220
BbrPI	CACGTG	1 cut(s)	265
BbsI	GAAGAC	1 cut(s)	204
Bbv12I	GWGCWC	2 cut(s)	1024, 1838
BbvCI	CCTCAGC	1 cut(s)	870
BbvI	GCAGC	9 cut(s)	121, 223, 507, 547, 571, 721, 1369, 1389, 1615
BccI	CCATC	3 cut(s)	8, 438, 555
BceAI	ACGGC	3 cut(s)	304, 334, 770
BcgI	CGANNNNNNTGC	2 cut(s)	1925, 1959
BciT130I	CCWGG	5 cut(s)	814, 1328, 1542, 1579, 1602
BcnI	CCSGG	1 cut(s)	465
BcoDI	GTCTC	5 cut(s)	210, 408, 1033, 1137, 1507
BcuI	ACTAGT	1 cut(s)	608
BfaI	CTAG	6 cut(s)	138, 609, 831, 941, 1029, 1632
BfmI	CTRYAG	4 cut(s)	585, 684, 1140, 1500
BglII	AGATCT	1 cut(s)	277
BlpI	GCTNAGC	1 cut(s)	142
Bme1390I	CCNGG	6 cut(s)	465, 814, 1328, 1542, 1579, 1602
Bme18I	GGWCC	4 cut(s)	327, 1250, 1635, 1709
BmeRI	GACNNNNNGTC	1 cut(s)	724
BmeT110I	CYCGRG	2 cut(s)	993, 2181
BmgBI	CACGTC	1 cut(s)	229
BmgT120I	GGNCC	4 cut(s)	327, 1250, 1635, 1709
BmiI	GGNNCC	4 cut(s)	19, 1416, 1637, 1710
BmrFI	CCNGG	6 cut(s)	465, 814, 1328, 1542, 1579, 1602
BmrI	ACTGGG	1 cut(s)	1004
BmsI	GCATC	7 cut(s)	523, 571, 663, 746, 1543, 1658, 2226
BmtI	GCTAGC	1 cut(s)	141
BmuI	ACTGGG	1 cut(s)	1004
BoxI	GACNNNNGTC	2 cut(s)	188, 693
BpiI	GAAGAC	1 cut(s)	204
BpmI	CTGGAG	2 cut(s)	1563, 1758
Bpu10I	CCTNAGC	1 cut(s)	870
Bpu1102I	GCTNAGC	1 cut(s)	142
Bpu14I	TTCGAA	1 cut(s)	986
BpuMI	CCSGG	1 cut(s)	465
BsaAI	YACGTR	2 cut(s)	265, 2087
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	2 cut(s)	210, 408
BsaJI	CCNNGG	5 cut(s)	186, 463, 752, 813, 1908
BsaWI	WCCGGW	1 cut(s)	329
Bsc4I	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 553, 677, 1327, 1370, 1845, 2017
Bse118I	RCCGGY	2 cut(s)	446, 856
Bse1I	ACTGG	4 cut(s)	1010, 1301, 1775, 1971
Bse21I	CCTNAGG	1 cut(s)	552
BseBI	CCWGG	5 cut(s)	814, 1328, 1542, 1579, 1602
BseDI	CCNNGG	5 cut(s)	186, 463, 752, 813, 1908
BseGI	GGATG	3 cut(s)	547, 667, 1729
BseLI	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 553, 677, 1327, 1370, 1845, 2017
BseMII	CTCAG	5 cut(s)	156, 221, 473, 543, 884
BseNI	ACTGG	4 cut(s)	1010, 1301, 1775, 1971
BseRI	GAGGAG	1 cut(s)	88
BseSI	GKGCMC	2 cut(s)	1009, 1024
BseXI	GCAGC	9 cut(s)	121, 223, 507, 547, 571, 721, 1369, 1389, 1615
BseYI	CCCAGC	2 cut(s)	27, 130
BsgI	GTGCAG	3 cut(s)	546, 937, 1724
Bsh1236I	CGCG	3 cut(s)	1574, 1935, 2156
BshFI	GGCC	2 cut(s)	250, 291
BsiHKAI	GWGCWC	2 cut(s)	1024, 1838
BsiHKCI	CYCGRG	2 cut(s)	993, 2181
BsiSI	CCGG	4 cut(s)	330, 447, 465, 857
BslFI	GGGAC	1 cut(s)	1890
BslI	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 553, 677, 1327, 1370, 1845, 2017
BsmAI	GTCTC	5 cut(s)	210, 408, 1033, 1137, 1507
BsmBI	CGTCTC	1 cut(s)	1137
BsmFI	GGGAC	1 cut(s)	1890
BsmI	GAATGC	1 cut(s)	1246
BsnI	GGCC	2 cut(s)	250, 291
Bso31I	GGTCTC	2 cut(s)	210, 408
BsoBI	CYCGRG	2 cut(s)	993, 2181
Bsp119I	TTCGAA	1 cut(s)	986
Bsp1286I	GDGCHC	4 cut(s)	131, 1009, 1024, 1838
Bsp143I	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
Bsp1720I	GCTNAGC	1 cut(s)	142
Bsp68I	TCGCGA	1 cut(s)	1935
BspACI	CCGC	6 cut(s)	5, 257, 389, 506, 524, 2156
BspANI	GGCC	2 cut(s)	250, 291
BspCNI	CTCAG	5 cut(s)	155, 220, 474, 544, 883
BspFNI	CGCG	3 cut(s)	1574, 1935, 2156
BspLI	GGNNCC	4 cut(s)	19, 1416, 1637, 1710
BspMAI	CTGCAG	1 cut(s)	589
BspOI	GCTAGC	1 cut(s)	141
BspPI	GGATC	4 cut(s)	380, 844, 1292, 1480
BspQI	GCTCTTC	2 cut(s)	504, 1826
BspT104I	TTCGAA	1 cut(s)	986
BspTNI	GGTCTC	2 cut(s)	210, 408
BsrFI	RCCGGY	2 cut(s)	446, 856
BsrI	ACTGG	4 cut(s)	1010, 1301, 1775, 1971
BssAI	RCCGGY	2 cut(s)	446, 856
BssECI	CCNNGG	5 cut(s)	186, 463, 752, 813, 1908
BssMI	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
BssNI	GRCGYC	1 cut(s)	303
BssSI	CACGAG	1 cut(s)	1220
BssT1I	CCWWGG	2 cut(s)	186, 1908
Bst2BI	CACGAG	1 cut(s)	1220
Bst2UI	CCWGG	5 cut(s)	814, 1328, 1542, 1579, 1602
Bst4CI	ACNGT	7 cut(s)	419, 935, 1273, 1741, 1798, 2080, 2133
Bst6I	CTCTTC	2 cut(s)	504, 1826
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	520
BstBAI	YACGTR	2 cut(s)	265, 2087
BstBI	TTCGAA	1 cut(s)	986
BstC8I	GCNNGC	3 cut(s)	139, 448, 470
BstDEI	CTNAG	5 cut(s)	142, 207, 482, 552, 870
BstDSI	CCRYGG	1 cut(s)	752
BstEII	GGTNACC	2 cut(s)	190, 1960
BstF5I	GGATG	3 cut(s)	547, 667, 1729
BstFNI	CGCG	3 cut(s)	1574, 1935, 2156
BstKTI	GATC	7 cut(s)	280, 375, 839, 1231, 1300, 1475, 1510
BstMAI	GTCTC	5 cut(s)	210, 408, 1033, 1137, 1507
BstMBI	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
BstMWI	GCNNNNNNNGC	4 cut(s)	143, 256, 265, 520
BstNI	CCWGG	5 cut(s)	814, 1328, 1542, 1579, 1602
BstPAI	GACNNNNGTC	2 cut(s)	188, 693
BstPI	GGTNACC	2 cut(s)	190, 1960
BstSCI	CCNGG	6 cut(s)	463, 812, 1326, 1540, 1577, 1600
BstSFI	CTRYAG	4 cut(s)	585, 684, 1140, 1500
BstSLI	GKGCMC	2 cut(s)	1009, 1024
BstUI	CGCG	3 cut(s)	1574, 1935, 2156
BstV1I	GCAGC	9 cut(s)	121, 223, 507, 547, 571, 721, 1369, 1389, 1615
BstV2I	GAAGAC	1 cut(s)	204
BstX2I	RGATCY	2 cut(s)	277, 372
BstXI	CCANNNNNNTGG	1 cut(s)	1978
BstYI	RGATCY	2 cut(s)	277, 372
Bsu36I	CCTNAGG	1 cut(s)	552
BsuRI	GGCC	2 cut(s)	250, 291
BtgI	CCRYGG	1 cut(s)	752
BtrI	CACGTC	1 cut(s)	229
BtsCI	GGATG	3 cut(s)	547, 667, 1729
BtsI	GCAGTG	3 cut(s)	804, 829, 1300
BtsIMutI	CAGTG	6 cut(s)	215, 804, 829, 1300, 1794, 1795
BtuMI	TCGCGA	1 cut(s)	1935
Cac8I	GCNNGC	3 cut(s)	139, 448, 470
CaiI	CAGNNNCTG	3 cut(s)	217, 584, 734
Cfr10I	RCCGGY	2 cut(s)	446, 856
Cfr13I	GGNCC	4 cut(s)	327, 1250, 1635, 1709
CseI	GACGC	5 cut(s)	311, 683, 1563, 1580, 2162
Csp6I	GTAC	1 cut(s)	178
CspCI	CAANNNNNGTGG	4 cut(s)	741, 776, 1675, 1710
CviAII	CATG	5 cut(s)	1188, 1289, 1345, 1689, 1720
CviQI	GTAC	1 cut(s)	178
DdeI	CTNAG	5 cut(s)	142, 207, 482, 552, 870
DpnI	GATC	7 cut(s)	279, 374, 838, 1230, 1299, 1474, 1509
DpnII	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
DriI	GACNNNNNGTC	1 cut(s)	724
EaeI	YGGCCR	1 cut(s)	289
Eam1104I	CTCTTC	2 cut(s)	504, 1826
Eam1105I	GACNNNNNGTC	1 cut(s)	724
EarI	CTCTTC	2 cut(s)	504, 1826
Ecl136II	GAGCTC	1 cut(s)	1836
Eco130I	CCWWGG	2 cut(s)	186, 1908
Eco147I	AGGCCT	1 cut(s)	250
Eco24I	GRGCYC	2 cut(s)	131, 1838
Eco31I	GGTCTC	2 cut(s)	210, 408
Eco32I	GATATC	1 cut(s)	1989
Eco47I	GGWCC	4 cut(s)	327, 1250, 1635, 1709
Eco53kI	GAGCTC	1 cut(s)	1836
Eco57I	CTGAAG	5 cut(s)	474, 561, 1235, 1389, 1542
Eco72I	CACGTG	1 cut(s)	265
Eco81I	CCTNAGG	1 cut(s)	552
Eco88I	CYCGRG	2 cut(s)	993, 2181
Eco91I	GGTNACC	2 cut(s)	190, 1960
EcoICRI	GAGCTC	1 cut(s)	1836
EcoO109I	RGGNCCY	1 cut(s)	1635
EcoO65I	GGTNACC	2 cut(s)	190, 1960
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	5 cut(s)	812, 1326, 1540, 1577, 1600
EcoRV	GATATC	1 cut(s)	1989
EcoT14I	CCWWGG	2 cut(s)	186, 1908
EcoT38I	GRGCYC	2 cut(s)	131, 1838
ErhI	CCWWGG	2 cut(s)	186, 1908
Esp3I	CGTCTC	1 cut(s)	1137
FaeI	CATG	5 cut(s)	1191, 1292, 1348, 1692, 1723
FalI	AAGNNNNNCTT	2 cut(s)	1740, 1772
FaqI	GGGAC	1 cut(s)	1890
FatI	CATG	5 cut(s)	1187, 1288, 1344, 1688, 1719
FauI	CCCGC	1 cut(s)	396
FblI	GTMKAC	1 cut(s)	300
FokI	GGATG	3 cut(s)	534, 674, 1736
FriOI	GRGCYC	2 cut(s)	131, 1838
FspBI	CTAG	6 cut(s)	138, 609, 831, 941, 1029, 1632
GsaI	CCCAGC	2 cut(s)	31, 134
GsuI	CTGGAG	2 cut(s)	1563, 1758
HaeIII	GGCC	2 cut(s)	250, 291
HapII	CCGG	4 cut(s)	330, 447, 465, 857
HgaI	GACGC	5 cut(s)	311, 683, 1563, 1580, 2162
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	5 cut(s)	1191, 1292, 1348, 1692, 1723
HincII	GTYRAC	2 cut(s)	301, 1753
HindII	GTYRAC	2 cut(s)	301, 1753
HpaI	GTTAAC	1 cut(s)	1753
HpaII	CCGG	4 cut(s)	330, 447, 465, 857
HphI	GGTGA	4 cut(s)	184, 911, 947, 1972
Hpy166II	GTNNAC	5 cut(s)	301, 327, 1022, 1753, 2098
Hpy8I	GTNNAC	5 cut(s)	301, 327, 1022, 1753, 2098
Hpy99I	CGWCG	3 cut(s)	83, 233, 305
HpyAV	CCTTC	6 cut(s)	157, 972, 982, 1055, 1325, 1364
HpyCH4III	ACNGT	7 cut(s)	419, 935, 1273, 1741, 1798, 2080, 2133
HpyCH4IV	ACGT	5 cut(s)	228, 264, 1277, 1869, 2086
HpyF10VI	GCNNNNNNNGC	4 cut(s)	143, 256, 265, 520
HpyF3I	CTNAG	5 cut(s)	142, 207, 482, 552, 870
HpySE526I	ACGT	5 cut(s)	228, 264, 1277, 1869, 2086
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	5 cut(s)	1191, 1292, 1348, 1692, 1723
KroI	GCCGGC	1 cut(s)	446
KroNI	GCCGGC	1 cut(s)	448
KspAI	GTTAAC	1 cut(s)	1753
Kzo9I	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
LguI	GCTCTTC	2 cut(s)	504, 1826
LmnI	GCTCC	6 cut(s)	23, 75, 98, 631, 1841, 2173
Lsp1109I	GCAGC	9 cut(s)	121, 223, 507, 547, 571, 721, 1369, 1389, 1615
LweI	GCATC	7 cut(s)	523, 571, 663, 746, 1543, 1658, 2226
MaeI	CTAG	6 cut(s)	138, 609, 831, 941, 1029, 1632
MaeII	ACGT	5 cut(s)	228, 264, 1277, 1869, 2086
MaeIII	GTNAC	6 cut(s)	190, 745, 1273, 1790, 1960, 2074
MalI	GATC	7 cut(s)	279, 374, 838, 1230, 1299, 1474, 1509
MboI	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
MfeI	CAATTG	2 cut(s)	758, 1854
MflI	RGATCY	2 cut(s)	277, 372
MhlI	GDGCHC	4 cut(s)	131, 1009, 1024, 1838
MluCI	AATT	7 cut(s)	404, 707, 758, 797, 1727, 1815, 1854
MluI	ACGCGT	1 cut(s)	1572
MlyI	GAGTC	5 cut(s)	420, 1154, 1464, 2002, 2050
MmeI	TCCRAC	2 cut(s)	567, 979
MroNI	GCCGGC	1 cut(s)	446
MroXI	GAANNNNTTC	1 cut(s)	982
MseI	TTAA	7 cut(s)	710, 864, 912, 1353, 1730, 1752, 1914
MslI	CAYNNNNRTG	2 cut(s)	1293, 2004
MspA1I	CMGCKG	4 cut(s)	134, 1178, 1283, 1592
MspI	CCGG	4 cut(s)	330, 447, 465, 857
MspR9I	CCNGG	6 cut(s)	465, 814, 1328, 1542, 1579, 1602
MunI	CAATTG	2 cut(s)	758, 1854
Mva1269I	GAATGC	1 cut(s)	1246
MvaI	CCWGG	5 cut(s)	814, 1328, 1542, 1579, 1602
MvnI	CGCG	3 cut(s)	1574, 1935, 2156
MwoI	GCNNNNNNNGC	4 cut(s)	143, 256, 265, 520
NaeI	GCCGGC	1 cut(s)	448
NciI	CCSGG	1 cut(s)	465
NdeII	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
NgoMIV	GCCGGC	1 cut(s)	446
NheI	GCTAGC	1 cut(s)	137
NlaIII	CATG	5 cut(s)	1191, 1292, 1348, 1692, 1723
NlaIV	GGNNCC	4 cut(s)	19, 1416, 1637, 1710
NmuCI	GTSAC	3 cut(s)	190, 1790, 1960
NruI	TCGCGA	1 cut(s)	1935
NspV	TTCGAA	1 cut(s)	986
PaeR7I	CTCGAG	2 cut(s)	993, 2181
PceI	AGGCCT	1 cut(s)	250
PciSI	GCTCTTC	2 cut(s)	504, 1826
PcsI	WCGNNNNNNNCGW	1 cut(s)	18
PctI	GAATGC	1 cut(s)	1246
PdiI	GCCGGC	1 cut(s)	448
PdmI	GAANNNNTTC	1 cut(s)	982
PfoI	TCCNGGA	3 cut(s)	1540, 1577, 1600
PleI	GAGTC	5 cut(s)	420, 1153, 1463, 2001, 2049
PmaCI	CACGTG	1 cut(s)	265
PmlI	CACGTG	1 cut(s)	265
PpsI	GAGTC	5 cut(s)	420, 1153, 1463, 2001, 2049
Ppu21I	YACGTR	2 cut(s)	265, 2087
PpuMI	RGGWCCY	1 cut(s)	1635
PshAI	GACNNNNGTC	2 cut(s)	188, 693
Psp124BI	GAGCTC	1 cut(s)	1838
Psp5II	RGGWCCY	1 cut(s)	1635
Psp6I	CCWGG	5 cut(s)	812, 1326, 1540, 1577, 1600
PspCI	CACGTG	1 cut(s)	265
PspEI	GGTNACC	2 cut(s)	190, 1960
PspFI	CCCAGC	2 cut(s)	27, 130
PspGI	CCWGG	5 cut(s)	812, 1326, 1540, 1577, 1600
PspN4I	GGNNCC	4 cut(s)	19, 1416, 1637, 1710
PspPI	GGNCC	4 cut(s)	327, 1250, 1635, 1709
PspPPI	RGGWCCY	1 cut(s)	1635
PspXI	VCTCGAGB	1 cut(s)	993
PstI	CTGCAG	1 cut(s)	589
PstNI	CAGNNNCTG	3 cut(s)	217, 584, 734
PsuI	RGATCY	2 cut(s)	277, 372
PvuII	CAGCTG	4 cut(s)	134, 1178, 1283, 1592
RruI	TCGCGA	1 cut(s)	1935
RsaI	GTAC	1 cut(s)	179
RsaNI	GTAC	1 cut(s)	178
RseI	CAYNNNNRTG	2 cut(s)	1293, 2004
SacI	GAGCTC	1 cut(s)	1838
SalI	GTCGAC	1 cut(s)	299
SapI	GCTCTTC	2 cut(s)	504, 1826
SaqAI	TTAA	7 cut(s)	710, 864, 912, 1353, 1730, 1752, 1914
Sau3AI	GATC	7 cut(s)	277, 372, 836, 1228, 1297, 1472, 1507
Sau96I	GGNCC	4 cut(s)	327, 1250, 1635, 1709
SchI	GAGTC	5 cut(s)	420, 1154, 1464, 2002, 2050
ScrFI	CCNGG	6 cut(s)	465, 814, 1328, 1542, 1579, 1602
SduI	GDGCHC	4 cut(s)	131, 1009, 1024, 1838
SfaNI	GCATC	7 cut(s)	523, 571, 663, 746, 1543, 1658, 2226
SfcI	CTRYAG	4 cut(s)	585, 684, 1140, 1500
Sfr274I	CTCGAG	2 cut(s)	993, 2181
SfuI	TTCGAA	1 cut(s)	986
SinI	GGWCC	4 cut(s)	327, 1250, 1635, 1709
SlaI	CTCGAG	2 cut(s)	993, 2181
SmiMI	CAYNNNNRTG	2 cut(s)	1293, 2004
SmlI	CTYRAG	2 cut(s)	993, 2181
SmoI	CTYRAG	2 cut(s)	993, 2181
SpeI	ACTAGT	1 cut(s)	608
Sse9I	AATT	7 cut(s)	404, 707, 758, 797, 1727, 1815, 1854
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	6 cut(s)	5, 257, 389, 506, 524, 2156
SspMI	CTAG	6 cut(s)	138, 609, 831, 941, 1029, 1632
SstI	GAGCTC	1 cut(s)	1838
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	6 cut(s)	463, 812, 1326, 1540, 1577, 1600
StyI	CCWWGG	2 cut(s)	186, 1908
TaaI	ACNGT	7 cut(s)	419, 935, 1273, 1741, 1798, 2080, 2133
TaiI	ACGT	5 cut(s)	231, 267, 1280, 1872, 2089
TasI	AATT	7 cut(s)	404, 707, 758, 797, 1727, 1815, 1854
TatI	WGTACW	1 cut(s)	177
TauI	GCSGC	2 cut(s)	259, 526
Tru1I	TTAA	7 cut(s)	710, 864, 912, 1353, 1730, 1752, 1914
Tru9I	TTAA	7 cut(s)	710, 864, 912, 1353, 1730, 1752, 1914
TscAI	CASTG	6 cut(s)	222, 811, 829, 1300, 1795, 1801
TseFI	GTSAC	3 cut(s)	190, 1790, 1960
TseI	GCWGC	9 cut(s)	134, 211, 520, 560, 584, 734, 1357, 1377, 1628
Tsp45I	GTSAC	3 cut(s)	190, 1790, 1960
TspDTI	ATGAA	4 cut(s)	1161, 1536, 1638, 1740
TspGWI	ACGGA	1 cut(s)	1310
TspRI	CASTG	6 cut(s)	222, 811, 829, 1300, 1795, 1801
VneI	GTGCAC	1 cut(s)	1020
VpaK11BI	GGWCC	4 cut(s)	327, 1250, 1635, 1709
XapI	RAATTY	1 cut(s)	404
XhoI	CTCGAG	2 cut(s)	993, 2181
XmiI	GTMKAC	1 cut(s)	300
XmnI	GAANNNNTTC	1 cut(s)	982
XspI	CTAG	6 cut(s)	138, 609, 831, 941, 1029, 1632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.