Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0004216

Physical Location & Seq

Genomic Coordinates Forward (+)

32035038 .. 32040885

Gene Structure 5848 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG314700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGGCGGGGTTCGATGGCTCCGAATCCCAGCAACAAAACCAGCTATTCAGCGTAGGGTCAGAAGTGGAAGTGAGGAGCGACGAGGACGGGTTCAAAGGAGCGTGGTTCAGAGCCACCATTGTTCAGAGCCCAGCTGCTAGCTCAGCCTCAAAGAAGAAGAAGAGGGCTCTGGTGGAGTACAAGACCTTGGTCACCGAAGACGGGTCTCAGCAGCCACTGAAAGAACAAGTCGATTCCGCTTACATAAGGCCTCTGCCGCCTCACGTGGCTGACCAAGATCTTGAATACGGCCACATAGTCGACGCCGACTACAAAGACGGTTGGTGGACCGGAGTTATTGACAAGGTTGTCAAGAAGAACTCCAAATATAGGATCTTCTTTGAGAACCCGCCCGACTTTCTGGAATTCGAGAGAGACCGTCTGCGACTCCATCAGGATTGGGTTGCCGGCTTTTGGGTTCGGCCCGACAAGCAGATACTGAGTGCTTCAGATTCTCAACAAGCGGAAGAGCATCGGCTGCGACTGCTGCCTGTCCAAAATGATTCCAACCATCCTGAGGTGGCTGCACAACCGAAAACTTCAGGTGCTGCAGGGGATAACCCTGAATCAACTAGTTCAAAGAAGAATCTTGTGGAGCAGTCAAGTTGTACCCAAAGCATCAAGGATGTAGCGCCTAATGGTGGTGCTACAGACTCGCGTCCTTTGAAAAAGTTAAAGGACGACAAAGTGTCAGAGGCTACATTATCTGTTACACCACGGCAATTGAGGAAAACTCCAAATGATAAAGAAATGCTATCTGAATTAGCCACTGCGACCAGGGAAAGCAGTGGAACTAGAGGATCAAGACGAGTAAGAAAACCGGCTGTTAGTCATCAGCTTTTTCACGTAGAGAGCTTGGTTGGTGAAAAGAAAGTTAAGACAAAGCAAGAGAAAGATGGTGAGCTAGAAAACCACTGGATGCACTCTGCCAAAAGTAAAGGAAGGCGTTCGAAGGCTCAAGTTGGAAGTGCCCAGGAAGCGTTTATATGTGCGCTTCCTAGCCAAGAGAGACATAAGGAAAAAGGTAAGGAAGGGTATGGAGTTATTGGTTCTGCTGGAAATGTTGTTCAAAAGGAGGGCATAAGTAAAGAAGCTGAATTGTCTCTCACTATAGAGTCAAAGGTTAATGGAAAGCAGGATTCATCAGCTGAATATCCATGTCAGATTCCCAACGAAGATTTGCTGAAGCTCGTGAGAGATCAGAAGAAGAATGCAAATGGTCCAGCACAAGAAAACAGCACGGATTTTAAGCAGCAACCAACTGAAGGAAGCAGCCACAAAAGAAAGAGGGGCAGACCTAGAAAGGTTCCTAAAAACTTTAGCTCACAGGGAGTCAGGGAACTAAATGGATCAAGAAATGTTGTTGACAAAAGTGCTGTAGAAGATCAGACACTTGATGAAGTTGCTTTGCATTTTCCCGGAGGGAGAGATGCTTCAGATTCACAAGATGCGTCCAGGAGAAATACAGCTGATGTTCCTGGAACAAGGGGCATACCAAATGAAGCTGCTAGGACCCGTGTTCAAGCATCGGAAAATATAGATGATGATGACCAACCTCTCTCCACATGGTTTGGAGGAATGCAGGGTCCAGCAAACATGGAAGAATTAAGTAAAACTGTTGAACAAGTTAAGCTTGCCAGAGATTCTACTCCAGTTGATTCAGTCAGTGACACTGTGCCAGATGAGAACCAAATTATGCCATTTGTGAAGAGCTCCCCAATCTGGAAAGCAATTGAATCATTTGACGTATTCAGAATAATGCCACAAAATCCACATTTTCGTCCCTTGGTTAAATGTAAAGAGGAATATCGCGAGGGATTTGCTATTGGCAATATGGTGACCTTTTCCAGTTTGGTGGAGAAGATATCGAGTCTGCATTTTGATGACCCTATACAGGTTTTTGATAGCATTTTGGAGAGTCTTTTTGACTTGGAAAAATATGGATTCAATGTCACCGTTTTACGTGGGCGTGTGAACGAACTTTTGTCTATCAAACAAAGGCAAGGACAGTTTGAGGGCGAATCAAAAGACGCGGAAAGTAAGATAGTGGAGCAAACTCAAGAGAAATCAAAACTTGAAGATGAGGCTGATTGCATCGAGAAAAAAATAATTGAGTTAAAGGAGAAGCATGCATCGATCCAGGCAGAGATAGGGGCTAAAGAGCGTGACATTGCTAGCTTGCAGTTGAGTCTGGATGCTATCAATGAAAGCATTAAGAACACACGTTCTGATTTTGAGAAGGCAGCTTTGGTCCAATTGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046983

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Agenet DUF724

Protein Analysis

767

Amino Acids

85.52

Weight (kDa)

6.05

Isoelectric Point (pI)

44.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Agenet	PF05641	19 - 87	8.8e-19	Agenet domain
DUF724	PF05266	579 - 762	1.4e-61	Protein of unknown function (DUF724)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004216)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g26800 FvH4_1g26800 FvH4_1g26800 FvH4_1g26800
malus_domestica	MD09G1286400.v1.1 MD17G1278800.v1.1
prunus_persica	Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1
pyrus_communis	pycom09g19350 pycom17g27640
rosa_chinensis	RchiOBHm_Chr3g0487021
rosa_laevigata	RLG00000023038
rosa_multiflora	Rmu_co8314393.1_g000001 Rmu_sc0002834.1_g000013 Rmu_sc0005856.1_g000019 Rmu_sc0012853.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395240
rosa_rugosa	Rorug03G0229700 Rorug03G0229800 Rorug03G0229900 Rorug03G0230000 Rorug03G0230100
rosa_samantha	Rh3AG278800 Rh3BG314700 Rh3CG313000 Rh3DG309600
rosa_wichuraiana	Rw3G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	347
AccI	GTMKAC	1 cut(s)	300
AccII	CGCG	3 cut(s)	697, 1851, 2072
AciI	CCGC	6 cut(s)	5, 237, 257, 389, 503, 2072
AclWI	GGATC	4 cut(s)	380, 847, 1396, 2170
AcoI	YGGCCR	1 cut(s)	289
AcsI	RAATTY	1 cut(s)	404
AcuI	CTGAAG	5 cut(s)	471, 564, 1244, 1323, 1458
AcvI	CACGTG	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	2 cut(s)	179, 649
AfiI	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1761, 1933
AflIII	ACRYGT	1 cut(s)	2261
AhdI	GACNNNNNGTC	1 cut(s)	727
AhlI	ACTAGT	1 cut(s)	611
AjnI	CCWGG	5 cut(s)	815, 1011, 1493, 1516, 2178
AjuI	GAANNNNNNNTTGG	2 cut(s)	2270, 2302
AloI	GAACNNNNNNTCC	2 cut(s)	1089, 1121
Alw21I	GWGCWC	1 cut(s)	1754
Alw26I	GTCTC	4 cut(s)	210, 408, 1042, 1146
AlwI	GGATC	4 cut(s)	380, 847, 1396, 2170
AlwNI	CAGNNNCTG	3 cut(s)	217, 478, 587
AoxI	GGCC	3 cut(s)	248, 289, 461
ApoI	RAATTY	1 cut(s)	404
Asp700I	GAANNNNTTC	1 cut(s)	985
AspLEI	GCGC	2 cut(s)	673, 1033
AspS9I	GGNCC	6 cut(s)	327, 462, 1259, 1551, 1625, 2290
AsuC2I	CCSGG	1 cut(s)	1458
AsuHPI	GGTGA	5 cut(s)	184, 914, 950, 1888, 1984
AsuII	TTCGAA	1 cut(s)	989
AsuNHI	GCTAGC	2 cut(s)	137, 2213
AvaII	GGWCC	5 cut(s)	327, 1259, 1551, 1625, 2290
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	1 cut(s)	1012
BanII	GRGCYC	3 cut(s)	131, 169, 1754
BauI	CACGAG	1 cut(s)	1229
BbrPI	CACGTG	1 cut(s)	265
BbsI	GAAGAC	1 cut(s)	204
Bbv12I	GWGCWC	1 cut(s)	1754
BccI	CCATC	4 cut(s)	8, 438, 558, 929
BceAI	ACGGC	2 cut(s)	304, 773
BcgI	CGANNNNNNTGC	2 cut(s)	1841, 1875
BciT130I	CCWGG	5 cut(s)	817, 1013, 1495, 1518, 2180
BcnI	CCSGG	1 cut(s)	1458
BcoDI	GTCTC	4 cut(s)	210, 408, 1042, 1146
BcuI	ACTAGT	1 cut(s)	611
BfaI	CTAG	8 cut(s)	138, 612, 834, 944, 1038, 1338, 1548, 2214
BfmI	CTRYAG	4 cut(s)	588, 687, 1149, 1416
BfoI	RGCGCY	1 cut(s)	674
BglII	AGATCT	1 cut(s)	277
BlpI	GCTNAGC	1 cut(s)	142
Bme1390I	CCNGG	6 cut(s)	817, 1013, 1458, 1495, 1518, 2180
Bme18I	GGWCC	5 cut(s)	327, 1259, 1551, 1625, 2290
BmeRI	GACNNNNNGTC	1 cut(s)	727
BmgT120I	GGNCC	6 cut(s)	327, 462, 1259, 1551, 1625, 2290
BmiI	GGNNCC	4 cut(s)	19, 1347, 1553, 1626
BmrFI	CCNGG	6 cut(s)	817, 1013, 1458, 1495, 1518, 2180
BmsI	GCATC	9 cut(s)	520, 666, 948, 1459, 1477, 1574, 2142, 2180, 2224
BmtI	GCTAGC	2 cut(s)	141, 2217
BoxI	GACNNNNGTC	2 cut(s)	188, 696
BpiI	GAAGAC	1 cut(s)	204
BplI	GAGNNNNNCTC	2 cut(s)	757, 789
BpmI	CTGGAG	1 cut(s)	1674
Bpu1102I	GCTNAGC	1 cut(s)	142
Bpu14I	TTCGAA	1 cut(s)	989
BpuEI	CTTGAG	2 cut(s)	981, 2082
BpuMI	CCSGG	1 cut(s)	1458
Bsa29I	ATCGAT	1 cut(s)	2174
BsaAI	YACGTR	3 cut(s)	265, 886, 2003
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	2 cut(s)	210, 408
BsaJI	CCNNGG	5 cut(s)	186, 755, 816, 1011, 1824
BsaWI	WCCGGW	1 cut(s)	329
Bsc4I	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1761, 1933
Bse118I	RCCGGY	2 cut(s)	446, 859
Bse1I	ACTGG	3 cut(s)	959, 1691, 1887
Bse21I	CCTNAGG	1 cut(s)	555
Bse3DI	GCAATG	1 cut(s)	2208
BseBI	CCWGG	5 cut(s)	817, 1013, 1495, 1518, 2180
BseCI	ATCGAT	1 cut(s)	2174
BseDI	CCNNGG	5 cut(s)	186, 755, 816, 1011, 1824
BseGI	GGATG	4 cut(s)	550, 670, 963, 2239
BseLI	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1761, 1933
BseMI	GCAATG	1 cut(s)	2208
BseMII	CTCAG	4 cut(s)	156, 221, 470, 546
BseNI	ACTGG	3 cut(s)	959, 1691, 1887
BseRI	GAGGAG	1 cut(s)	88
BseSI	GKGCMC	1 cut(s)	1012
BseYI	CCCAGC	2 cut(s)	27, 130
BsgI	GTGCAG	1 cut(s)	549
Bsh1236I	CGCG	3 cut(s)	697, 1851, 2072
BshFI	GGCC	3 cut(s)	250, 291, 463
BshVI	ATCGAT	1 cut(s)	2174
BsiHKAI	GWGCWC	1 cut(s)	1754
BsiSI	CCGG	4 cut(s)	330, 447, 860, 1458
BslFI	GGGAC	1 cut(s)	1806
BslI	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1761, 1933
BsmAI	GTCTC	4 cut(s)	210, 408, 1042, 1146
BsmFI	GGGAC	1 cut(s)	1806
BsmI	GAATGC	2 cut(s)	1255, 1623
BsnI	GGCC	3 cut(s)	250, 291, 463
Bso31I	GGTCTC	2 cut(s)	210, 408
Bsp119I	TTCGAA	1 cut(s)	989
Bsp1286I	GDGCHC	4 cut(s)	131, 169, 1012, 1754
Bsp143I	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
Bsp1720I	GCTNAGC	1 cut(s)	142
Bsp68I	TCGCGA	1 cut(s)	1851
BspACI	CCGC	6 cut(s)	5, 237, 257, 389, 503, 2072
BspANI	GGCC	3 cut(s)	250, 291, 463
BspCNI	CTCAG	4 cut(s)	155, 220, 471, 547
BspDI	ATCGAT	1 cut(s)	2174
BspFNI	CGCG	3 cut(s)	697, 1851, 2072
BspLI	GGNNCC	4 cut(s)	19, 1347, 1553, 1626
BspMAI	CTGCAG	1 cut(s)	592
BspOI	GCTAGC	2 cut(s)	141, 2217
BspPI	GGATC	4 cut(s)	380, 847, 1396, 2170
BspQI	GCTCTTC	2 cut(s)	501, 1742
BspT104I	TTCGAA	1 cut(s)	989
BspTNI	GGTCTC	2 cut(s)	210, 408
BsrDI	GCAATG	1 cut(s)	2208
BsrFI	RCCGGY	2 cut(s)	446, 859
BsrI	ACTGG	3 cut(s)	959, 1691, 1887
BssAI	RCCGGY	2 cut(s)	446, 859
BssECI	CCNNGG	5 cut(s)	186, 755, 816, 1011, 1824
BssMI	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
BssNI	GRCGYC	1 cut(s)	303
BssSI	CACGAG	1 cut(s)	1229
BssT1I	CCWWGG	2 cut(s)	186, 1824
Bst2BI	CACGAG	1 cut(s)	1229
Bst2UI	CCWGG	5 cut(s)	817, 1013, 1495, 1518, 2180
Bst4CI	ACNGT	6 cut(s)	320, 419, 1657, 1714, 1996, 2049
Bst6I	CTCTTC	3 cut(s)	155, 501, 1742
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	517
BstBAI	YACGTR	3 cut(s)	265, 886, 2003
BstBI	TTCGAA	1 cut(s)	989
BstC8I	GCNNGC	6 cut(s)	139, 448, 1674, 2169, 2215, 2219
BstDEI	CTNAG	4 cut(s)	142, 207, 479, 555
BstDSI	CCRYGG	1 cut(s)	755
BstEII	GGTNACC	2 cut(s)	190, 1876
BstF5I	GGATG	4 cut(s)	550, 670, 963, 2239
BstFNI	CGCG	3 cut(s)	697, 1851, 2072
BstH2I	RGCGCY	1 cut(s)	674
BstHHI	GCGC	2 cut(s)	673, 1033
BstKTI	GATC	7 cut(s)	280, 375, 842, 1240, 1391, 1426, 2178
BstMAI	GTCTC	4 cut(s)	210, 408, 1042, 1146
BstMBI	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
BstMWI	GCNNNNNNNGC	7 cut(s)	143, 256, 469, 517, 523, 526, 1016
BstNI	CCWGG	5 cut(s)	817, 1013, 1495, 1518, 2180
BstNSI	RCATGY	1 cut(s)	2171
BstPAI	GACNNNNGTC	2 cut(s)	188, 696
BstPI	GGTNACC	2 cut(s)	190, 1876
BstSCI	CCNGG	6 cut(s)	815, 1011, 1456, 1493, 1516, 2178
BstSFI	CTRYAG	4 cut(s)	588, 687, 1149, 1416
BstSLI	GKGCMC	1 cut(s)	1012
BstUI	CGCG	3 cut(s)	697, 1851, 2072
BstV2I	GAAGAC	1 cut(s)	204
BstX2I	RGATCY	2 cut(s)	277, 372
BstXI	CCANNNNNNTGG	1 cut(s)	1894
BstYI	RGATCY	2 cut(s)	277, 372
Bsu15I	ATCGAT	1 cut(s)	2174
Bsu36I	CCTNAGG	1 cut(s)	555
BsuRI	GGCC	3 cut(s)	250, 291, 463
BsuTUI	ATCGAT	1 cut(s)	2174
BtgI	CCRYGG	1 cut(s)	755
BtsCI	GGATG	4 cut(s)	550, 670, 963, 2239
BtsI	GCAGTG	2 cut(s)	807, 832
BtsIMutI	CAGTG	6 cut(s)	215, 807, 832, 952, 1710, 1711
BtuMI	TCGCGA	1 cut(s)	1851
Cac8I	GCNNGC	6 cut(s)	139, 448, 1674, 2169, 2215, 2219
CaiI	CAGNNNCTG	3 cut(s)	217, 478, 587
CfoI	GCGC	2 cut(s)	673, 1033
Cfr10I	RCCGGY	2 cut(s)	446, 859
Cfr13I	GGNCC	6 cut(s)	327, 462, 1259, 1551, 1625, 2290
ClaI	ATCGAT	1 cut(s)	2174
CseI	GACGC	4 cut(s)	311, 686, 1479, 2078
Csp6I	GTAC	2 cut(s)	178, 648
CspCI	CAANNNNNGTGG	4 cut(s)	744, 779, 1591, 1626
CviAII	CATG	4 cut(s)	1197, 1605, 1636, 2168
CviQI	GTAC	2 cut(s)	178, 648
DdeI	CTNAG	4 cut(s)	142, 207, 479, 555
DpnI	GATC	7 cut(s)	279, 374, 841, 1239, 1390, 1425, 2177
DpnII	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
DrdI	GACNNNNNNGTC	1 cut(s)	347
DriI	GACNNNNNGTC	1 cut(s)	727
DseDI	GACNNNNNNGTC	1 cut(s)	347
EaeI	YGGCCR	1 cut(s)	289
Eam1104I	CTCTTC	3 cut(s)	155, 501, 1742
Eam1105I	GACNNNNNGTC	1 cut(s)	727
EarI	CTCTTC	3 cut(s)	155, 501, 1742
Ecl136II	GAGCTC	1 cut(s)	1752
Eco130I	CCWWGG	2 cut(s)	186, 1824
Eco147I	AGGCCT	1 cut(s)	250
Eco24I	GRGCYC	3 cut(s)	131, 169, 1754
Eco31I	GGTCTC	2 cut(s)	210, 408
Eco32I	GATATC	1 cut(s)	1905
Eco47I	GGWCC	5 cut(s)	327, 1259, 1551, 1625, 2290
Eco53kI	GAGCTC	1 cut(s)	1752
Eco57I	CTGAAG	5 cut(s)	471, 564, 1244, 1323, 1458
Eco72I	CACGTG	1 cut(s)	265
Eco81I	CCTNAGG	1 cut(s)	555
Eco91I	GGTNACC	2 cut(s)	190, 1876
EcoICRI	GAGCTC	1 cut(s)	1752
EcoO109I	RGGNCCY	1 cut(s)	1551
EcoO65I	GGTNACC	2 cut(s)	190, 1876
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	5 cut(s)	815, 1011, 1493, 1516, 2178
EcoRV	GATATC	1 cut(s)	1905
EcoT14I	CCWWGG	2 cut(s)	186, 1824
EcoT22I	ATGCAT	1 cut(s)	2173
EcoT38I	GRGCYC	3 cut(s)	131, 169, 1754
ErhI	CCWWGG	2 cut(s)	186, 1824
FaeI	CATG	4 cut(s)	1200, 1608, 1639, 2171
FalI	AAGNNNNNCTT	2 cut(s)	1656, 1688
FaqI	GGGAC	1 cut(s)	1806
FatI	CATG	4 cut(s)	1196, 1604, 1635, 2167
FauI	CCCGC	1 cut(s)	396
FblI	GTMKAC	1 cut(s)	300
FokI	GGATG	4 cut(s)	537, 677, 970, 2246
FriOI	GRGCYC	3 cut(s)	131, 169, 1754
FspBI	CTAG	8 cut(s)	138, 612, 834, 944, 1038, 1338, 1548, 2214
GlaI	GCGC	2 cut(s)	672, 1032
GsaI	CCCAGC	2 cut(s)	31, 134
GsuI	CTGGAG	1 cut(s)	1674
HaeII	RGCGCY	1 cut(s)	674
HaeIII	GGCC	3 cut(s)	250, 291, 463
HapII	CCGG	4 cut(s)	330, 447, 860, 1458
HgaI	GACGC	4 cut(s)	311, 686, 1479, 2078
HhaI	GCGC	2 cut(s)	673, 1033
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	4 cut(s)	1200, 1608, 1639, 2171
Hin6I	GCGC	2 cut(s)	671, 1031
HinP1I	GCGC	2 cut(s)	671, 1031
HincII	GTYRAC	2 cut(s)	301, 1405
HindII	GTYRAC	2 cut(s)	301, 1405
HindIII	AAGCTT	1 cut(s)	1670
HpaII	CCGG	4 cut(s)	330, 447, 860, 1458
HphI	GGTGA	5 cut(s)	184, 914, 950, 1888, 1984
Hpy166II	GTNNAC	4 cut(s)	301, 327, 1405, 2014
Hpy8I	GTNNAC	4 cut(s)	301, 327, 1405, 2014
Hpy99I	CGWCG	2 cut(s)	83, 305
HpyAV	CCTTC	5 cut(s)	975, 985, 1064, 1298, 2272
HpyCH4III	ACNGT	6 cut(s)	320, 419, 1657, 1714, 1996, 2049
HpyCH4IV	ACGT	5 cut(s)	264, 885, 1785, 2002, 2263
HpyF10VI	GCNNNNNNNGC	7 cut(s)	143, 256, 469, 517, 523, 526, 1016
HpyF3I	CTNAG	4 cut(s)	142, 207, 479, 555
HpySE526I	ACGT	5 cut(s)	264, 885, 1785, 2002, 2263
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	4 cut(s)	1200, 1608, 1639, 2171
HspAI	GCGC	2 cut(s)	671, 1031
KroI	GCCGGC	1 cut(s)	446
KroNI	GCCGGC	1 cut(s)	448
Kzo9I	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
LguI	GCTCTTC	2 cut(s)	501, 1742
LmnI	GCTCC	6 cut(s)	23, 75, 98, 634, 1757, 2089
LweI	GCATC	9 cut(s)	520, 666, 948, 1459, 1477, 1574, 2142, 2180, 2224
MaeI	CTAG	8 cut(s)	138, 612, 834, 944, 1038, 1338, 1548, 2214
MaeII	ACGT	5 cut(s)	264, 885, 1785, 2002, 2263
MaeIII	GTNAC	6 cut(s)	190, 748, 1706, 1876, 1990, 2204
MalI	GATC	7 cut(s)	279, 374, 841, 1239, 1390, 1425, 2177
MboI	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
MfeI	CAATTG	3 cut(s)	761, 1770, 2294
MflI	RGATCY	2 cut(s)	277, 372
MhlI	GDGCHC	4 cut(s)	131, 169, 1012, 1754
MluCI	AATT	9 cut(s)	404, 761, 800, 1136, 1643, 1731, 1770, 2148, 2294
MlyI	GAGTC	7 cut(s)	420, 686, 1163, 1380, 1918, 1966, 2236
MmeI	TCCRAC	2 cut(s)	570, 982
Mph1103I	ATGCAT	1 cut(s)	2173
MroNI	GCCGGC	1 cut(s)	446
MroXI	GAANNNNTTC	1 cut(s)	985
MseI	TTAA	9 cut(s)	713, 915, 1164, 1287, 1646, 1668, 1830, 2156, 2253
MslI	CAYNNNNRTG	1 cut(s)	1920
MspA1I	CMGCKG	3 cut(s)	134, 1187, 1508
MspI	CCGG	4 cut(s)	330, 447, 860, 1458
MspR9I	CCNGG	6 cut(s)	817, 1013, 1458, 1495, 1518, 2180
MunI	CAATTG	3 cut(s)	761, 1770, 2294
Mva1269I	GAATGC	2 cut(s)	1255, 1623
MvaI	CCWGG	5 cut(s)	817, 1013, 1495, 1518, 2180
MvnI	CGCG	3 cut(s)	697, 1851, 2072
MwoI	GCNNNNNNNGC	7 cut(s)	143, 256, 469, 517, 523, 526, 1016
NaeI	GCCGGC	1 cut(s)	448
NciI	CCSGG	1 cut(s)	1458
NdeII	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
NgoMIV	GCCGGC	1 cut(s)	446
NheI	GCTAGC	2 cut(s)	137, 2213
NlaIII	CATG	4 cut(s)	1200, 1608, 1639, 2171
NlaIV	GGNNCC	4 cut(s)	19, 1347, 1553, 1626
NmuCI	GTSAC	5 cut(s)	190, 1706, 1876, 1990, 2204
NruI	TCGCGA	1 cut(s)	1851
NsiI	ATGCAT	1 cut(s)	2173
NspI	RCATGY	1 cut(s)	2171
NspV	TTCGAA	1 cut(s)	989
PaeI	GCATGC	1 cut(s)	2171
PceI	AGGCCT	1 cut(s)	250
PciSI	GCTCTTC	2 cut(s)	501, 1742
PcsI	WCGNNNNNNNCGW	1 cut(s)	18
PctI	GAATGC	2 cut(s)	1255, 1623
PdiI	GCCGGC	1 cut(s)	448
PdmI	GAANNNNTTC	1 cut(s)	985
PfoI	TCCNGGA	3 cut(s)	1456, 1493, 1516
PleI	GAGTC	7 cut(s)	420, 686, 1162, 1379, 1917, 1965, 2235
PmaCI	CACGTG	1 cut(s)	265
PmlI	CACGTG	1 cut(s)	265
PpsI	GAGTC	7 cut(s)	420, 686, 1162, 1379, 1917, 1965, 2235
Ppu21I	YACGTR	3 cut(s)	265, 886, 2003
PpuMI	RGGWCCY	1 cut(s)	1551
PshAI	GACNNNNGTC	2 cut(s)	188, 696
Psp124BI	GAGCTC	1 cut(s)	1754
Psp5II	RGGWCCY	1 cut(s)	1551
Psp6I	CCWGG	5 cut(s)	815, 1011, 1493, 1516, 2178
PspCI	CACGTG	1 cut(s)	265
PspEI	GGTNACC	2 cut(s)	190, 1876
PspFI	CCCAGC	2 cut(s)	27, 130
PspGI	CCWGG	5 cut(s)	815, 1011, 1493, 1516, 2178
PspN4I	GGNNCC	4 cut(s)	19, 1347, 1553, 1626
PspPI	GGNCC	6 cut(s)	327, 462, 1259, 1551, 1625, 2290
PspPPI	RGGWCCY	1 cut(s)	1551
PstI	CTGCAG	1 cut(s)	592
PstNI	CAGNNNCTG	3 cut(s)	217, 478, 587
PsuI	RGATCY	2 cut(s)	277, 372
PvuII	CAGCTG	3 cut(s)	134, 1187, 1508
RruI	TCGCGA	1 cut(s)	1851
RsaI	GTAC	2 cut(s)	179, 649
RsaNI	GTAC	2 cut(s)	178, 648
RseI	CAYNNNNRTG	1 cut(s)	1920
SacI	GAGCTC	1 cut(s)	1754
SalI	GTCGAC	1 cut(s)	299
SapI	GCTCTTC	2 cut(s)	501, 1742
SaqAI	TTAA	9 cut(s)	713, 915, 1164, 1287, 1646, 1668, 1830, 2156, 2253
Sau3AI	GATC	7 cut(s)	277, 372, 839, 1237, 1388, 1423, 2175
Sau96I	GGNCC	6 cut(s)	327, 462, 1259, 1551, 1625, 2290
SchI	GAGTC	7 cut(s)	420, 686, 1163, 1380, 1918, 1966, 2236
ScrFI	CCNGG	6 cut(s)	817, 1013, 1458, 1495, 1518, 2180
SduI	GDGCHC	4 cut(s)	131, 169, 1012, 1754
SfaNI	GCATC	9 cut(s)	520, 666, 948, 1459, 1477, 1574, 2142, 2180, 2224
SfcI	CTRYAG	4 cut(s)	588, 687, 1149, 1416
SfuI	TTCGAA	1 cut(s)	989
SinI	GGWCC	5 cut(s)	327, 1259, 1551, 1625, 2290
SmiMI	CAYNNNNRTG	1 cut(s)	1920
SmlI	CTYRAG	2 cut(s)	996, 2097
SmoI	CTYRAG	2 cut(s)	996, 2097
SpeI	ACTAGT	1 cut(s)	611
SphI	GCATGC	1 cut(s)	2171
Sse9I	AATT	9 cut(s)	404, 761, 800, 1136, 1643, 1731, 1770, 2148, 2294
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	6 cut(s)	5, 237, 257, 389, 503, 2072
SspMI	CTAG	8 cut(s)	138, 612, 834, 944, 1038, 1338, 1548, 2214
SstI	GAGCTC	1 cut(s)	1754
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	6 cut(s)	815, 1011, 1456, 1493, 1516, 2178
StyI	CCWWGG	2 cut(s)	186, 1824
TaaI	ACNGT	6 cut(s)	320, 419, 1657, 1714, 1996, 2049
TaiI	ACGT	5 cut(s)	267, 888, 1788, 2005, 2266
TaqI	TCGA	8 cut(s)	12, 231, 300, 408, 989, 1907, 2136, 2174
TasI	AATT	9 cut(s)	404, 761, 800, 1136, 1643, 1731, 1770, 2148, 2294
TatI	WGTACW	1 cut(s)	177
TauI	GCSGC	1 cut(s)	259
Tru1I	TTAA	9 cut(s)	713, 915, 1164, 1287, 1646, 1668, 1830, 2156, 2253
Tru9I	TTAA	9 cut(s)	713, 915, 1164, 1287, 1646, 1668, 1830, 2156, 2253
TscAI	CASTG	6 cut(s)	222, 814, 832, 959, 1711, 1717
TseFI	GTSAC	5 cut(s)	190, 1706, 1876, 1990, 2204
Tsp45I	GTSAC	5 cut(s)	190, 1706, 1876, 1990, 2204
TspDTI	ATGAA	4 cut(s)	1170, 1452, 1554, 2259
TspGWI	ACGGA	1 cut(s)	1295
TspRI	CASTG	6 cut(s)	222, 814, 832, 959, 1711, 1717
VpaK11BI	GGWCC	5 cut(s)	327, 1259, 1551, 1625, 2290
XapI	RAATTY	1 cut(s)	404
XceI	RCATGY	1 cut(s)	2171
XmiI	GTMKAC	1 cut(s)	300
XmnI	GAANNNNTTC	1 cut(s)	985
XspI	CTAG	8 cut(s)	138, 612, 834, 944, 1038, 1338, 1548, 2214
Zsp2I	ATGCAT	1 cut(s)	2173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.