Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004216

Physical Location & Seq

Genomic Coordinates Reverse (-)

17094456 .. 17100362

Gene Structure 5907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023038

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGCGGGGTTCGATGGCTCCGAATCCCAGCAACAAAACCAGCTATTCAGCGTAGGGTCAGAAGTGGAAGTGAGGAGCGACGAGGACGGGTTCAAAGGAGCGTGGTTCAGAGCCACCATTGTTCAGAGCCCAGCTGCTAGCTCAGCCTCAAAGAAGAAGAAGAGGGCTCTGGTGGAGTACAAGACCTTGGTCACCGAAGACGGGTCTCAGCAGCCACTGAAAGAACACGTCGATTGCGGTTATATAAGGCCTCTGCCGCCTCACGTGCCTGACCAAGATCTTGAACACGGCCACATAGTCGACGCCGACTACAAAGACGGATGGTGGACCGGAGTTATTGACAAGGTTATCAAGAAGAACTCCAAATATAGGATCTTCTTTGAGAACCCGCCCGACTTTCTGGAATTCGAGAGAGAACGTCTGCGACTCCATCAGGATTGGGTTGCCGGCTTTTGGGTTCGCCCCGAGAAGCAGATACCGAGTGCTTCAGATTCTCAACAAGCAGAAGAGCATCGGCTGCGACTGCTGCCTGTCCAAAATGATTCCAACCATCCTGAGGTGGCTGCACAACCGAAAACTTCAGGTGCTGCAGGGGATAACCCTGAATCAACTAGTTCAAAGAAGAATCTTGTGGAGCAGTCAAGTTTTACCCAAAGCATCAGGGATGTAGCGCCTAATGGTGGTGCTACAGACTTGCGTCCTTTGAAAAAGTTAAAGGACGACAAAGTGTCAGAGGCTACATTATCTGTTACACCACGGCAATTGAGGAAAACTCCAAATGATAAAGAAATGCTATGTGAATTAGCCACTGCGACCAAGGAAAGCAGTGGAACTAGAGGATCAAGACGAGTAAGAAAACCGGCTGTTAGTCATCAGCTTTTTCACGTTGAGAGCTTGGTTGGTGAAAAGAAAGTTAAGACAAAGCAAGAGAAAGATGGTGAGCTAGAAAACCACTGGACGCACTCTGTCAAAAGTAAAGGAAGGCGTTCGAAGGCTCAAGTTGGAAGTGCCCAGGAAGCGTTTATATGTGCGCTTCCTAGCCAAGAGAGACATAAGGAAAAAGGTAAGGAAGGGTATGGAGTTATTGGTTCTGCTGGAAATGTCGTTCAAAAGGAGGGCATAAGTAAAGAAGCTGAATTGTCTCTCACTATAGAGTCAAAGGTCAATGGAAAGCAGGATTCATCAGCTGAATATCCATGTCAGATTCCCAACGAAGATTTGCTGAAGCTCATGAGAGATCAGAAGAAGAATGCAAATGGTCCAGCACAAGAAAACAGCACGGATTTTAAGCAGCAACCAACTGAAGGAAGCAGCCACAAAAGAAAGAGGGGCAGACCTAGAAAGGTTCCTAAAAACTTTATTGTAATCACACAAGCCTCACAGGGAGTCAGGGAACTAAATGGATCAAGAAATGTTGCTGACAAAAGTGCTGTAGAAGATCAGAGACTTGATGAAGTTGCTTTGCATTTGCCCGGAGGGAGAGATGCTTCAGATTCACACGATGCGTCCAGGAGAAATACAGCTGATGTTCCTGGATCAAGGGGCATACCAAATGAAGCTGCTAGGACCCGTGTTCAAGCATCGGAAAATATAGATGATGATGACCAACCTCTCTCCACATGGTTTGGAGGAATTCAGGGTCCAGCAAACATGGATGAATTAAGTAAAACTGTTGAACAAGTTAAGCTTGCCAGAGATTCTACTCCAGTTGATTCCGTCAGTGACACTGTGCCAGATGAGAACCAAATTATGCCATTTGTGAAGAGCTCCCCAATCTGGAAAGCAATTGAATCATTTGACGTATTCAGAATAATGCCACAAAATCCACATTTTCGTCCCTTGGTTAAATGTAAAGAGGAATATCGCGAGGGATTTGCAATTGGCAATATGGTGACCTTTTCCAGTTTGGTGGAGAAGATATCGAGTCTGCATTTTGATGACCCTATACAGGTTTTTGATAGCATTTTGGAGAGTCTTTTTGACTTGGAAAAATATGGATTCAATGTTACCGTTTTACGTGGGCGTGTGAACGAACTTTTGTCTATGAAACAAAGGCAAGGACAGTTCGAGGGCGAATCAAAAGACGCGGAAAGTAAGATAGTGGAGCAAACTCAAGAGAAATCAAAACTTGAAGATGAGGCTGATTGCATCGAGAAAAAAATAATTGAGTTAAAGGAGAAGCATGCATCAATCCAGGCAGAGATAGGGGCTAAAGAGCGTGACATTGCTAGCTTACAGTTGAGTGTGGATGCTATCAATGAAAGCATTAAGACCACACGTTCTGATTTTGAGAAGCTAGCTTTGGTCCCATTGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046983

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Agenet DUF724

Protein Analysis

773

Amino Acids

86.17

Weight (kDa)

6.21

Isoelectric Point (pI)

40.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Agenet	PF05641	19 - 87	3.4e-18	Agenet domain
DUF724	PF05266	584 - 768	7.6e-62	Protein of unknown function (DUF724)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004216)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g26800 FvH4_1g26800 FvH4_1g26800 FvH4_1g26800
malus_domestica	MD09G1286400.v1.1 MD17G1278800.v1.1
prunus_persica	Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1
pyrus_communis	pycom09g19350 pycom17g27640
rosa_chinensis	RchiOBHm_Chr3g0487021
rosa_laevigata	RLG00000023038
rosa_multiflora	Rmu_co8314393.1_g000001 Rmu_sc0002834.1_g000013 Rmu_sc0005856.1_g000019 Rmu_sc0012853.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395240
rosa_rugosa	Rorug03G0229700 Rorug03G0229800 Rorug03G0229900 Rorug03G0230000 Rorug03G0230100
rosa_samantha	Rh3AG278800 Rh3BG314700 Rh3CG313000 Rh3DG309600
rosa_wichuraiana	Rw3G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	300
AccII	CGCG	2 cut(s)	1866, 2087
AciI	CCGC	5 cut(s)	5, 237, 257, 389, 2087
AclWI	GGATC	4 cut(s)	380, 847, 1411, 1543
AcoI	YGGCCR	1 cut(s)	289
AcsI	RAATTY	2 cut(s)	404, 1632
AcuI	CTGAAG	5 cut(s)	471, 564, 1244, 1323, 1473
AcvI	CACGTG	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	1 cut(s)	179
AfiI	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1776, 1948
AflIII	ACRYGT	2 cut(s)	226, 2276
AhdI	GACNNNNNGTC	1 cut(s)	727
AhlI	ACTAGT	1 cut(s)	611
AjiI	CACGTC	1 cut(s)	229
AjnI	CCWGG	4 cut(s)	1011, 1508, 1531, 2193
AjuI	GAANNNNNNNTTGG	2 cut(s)	2285, 2317
AloI	GAACNNNNNNTCC	2 cut(s)	1089, 1121
Alw21I	GWGCWC	1 cut(s)	1769
Alw26I	GTCTC	4 cut(s)	210, 1042, 1146, 1438
AlwI	GGATC	4 cut(s)	380, 847, 1411, 1543
AlwNI	CAGNNNCTG	2 cut(s)	217, 587
Ama87I	CYCGRG	1 cut(s)	464
AoxI	GGCC	2 cut(s)	248, 289
ApeKI	GCWGC	9 cut(s)	134, 211, 517, 526, 563, 587, 1291, 1311, 1559
ApoI	RAATTY	2 cut(s)	404, 1632
Asp700I	GAANNNNTTC	1 cut(s)	985
AspLEI	GCGC	2 cut(s)	673, 1033
AspS9I	GGNCC	5 cut(s)	327, 1259, 1566, 1640, 2305
AsuC2I	CCSGG	1 cut(s)	1473
AsuHPI	GGTGA	4 cut(s)	184, 914, 950, 1903
AsuII	TTCGAA	1 cut(s)	989
AsuNHI	GCTAGC	3 cut(s)	137, 2228, 2296
AvaI	CYCGRG	1 cut(s)	464
AvaII	GGWCC	5 cut(s)	327, 1259, 1566, 1640, 2305
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	1 cut(s)	1012
BanII	GRGCYC	3 cut(s)	131, 169, 1769
BbrPI	CACGTG	1 cut(s)	265
BbsI	GAAGAC	1 cut(s)	204
Bbv12I	GWGCWC	1 cut(s)	1769
BbvI	GCAGC	9 cut(s)	121, 223, 504, 513, 550, 574, 1303, 1323, 1546
BccI	CCATC	5 cut(s)	8, 315, 438, 558, 929
BceAI	ACGGC	2 cut(s)	304, 773
BcgI	CGANNNNNNTGC	2 cut(s)	1856, 1890
BciT130I	CCWGG	4 cut(s)	1013, 1510, 1533, 2195
BcnI	CCSGG	1 cut(s)	1473
BcoDI	GTCTC	4 cut(s)	210, 1042, 1146, 1438
BcuI	ACTAGT	1 cut(s)	611
BfaI	CTAG	9 cut(s)	138, 612, 834, 944, 1038, 1338, 1563, 2229, 2297
BfmI	CTRYAG	4 cut(s)	588, 687, 1149, 1431
BfoI	RGCGCY	1 cut(s)	674
BglII	AGATCT	1 cut(s)	277
BlpI	GCTNAGC	1 cut(s)	142
Bme1390I	CCNGG	5 cut(s)	1013, 1473, 1510, 1533, 2195
Bme18I	GGWCC	5 cut(s)	327, 1259, 1566, 1640, 2305
BmeRI	GACNNNNNGTC	1 cut(s)	727
BmeT110I	CYCGRG	1 cut(s)	464
BmgBI	CACGTC	1 cut(s)	229
BmgT120I	GGNCC	5 cut(s)	327, 1259, 1566, 1640, 2305
BmiI	GGNNCC	5 cut(s)	19, 1347, 1568, 1641, 2307
BmrFI	CCNGG	5 cut(s)	1013, 1473, 1510, 1533, 2195
BmsI	GCATC	8 cut(s)	520, 666, 1474, 1492, 1589, 2157, 2195, 2239
BmtI	GCTAGC	3 cut(s)	141, 2232, 2300
BoxI	GACNNNNGTC	2 cut(s)	188, 696
BpiI	GAAGAC	1 cut(s)	204
BplI	GAGNNNNNCTC	2 cut(s)	757, 789
BpmI	CTGGAG	1 cut(s)	1689
Bpu1102I	GCTNAGC	1 cut(s)	142
Bpu14I	TTCGAA	1 cut(s)	989
BpuEI	CTTGAG	2 cut(s)	981, 2097
BpuMI	CCSGG	1 cut(s)	1473
BsaAI	YACGTR	2 cut(s)	265, 2018
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	1 cut(s)	210
BsaJI	CCNNGG	5 cut(s)	186, 755, 816, 1011, 1839
BsaWI	WCCGGW	1 cut(s)	329
Bsc4I	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1776, 1948
Bse118I	RCCGGY	2 cut(s)	446, 859
Bse1I	ACTGG	3 cut(s)	959, 1706, 1902
Bse21I	CCTNAGG	1 cut(s)	555
Bse3DI	GCAATG	1 cut(s)	2223
BseBI	CCWGG	4 cut(s)	1013, 1510, 1533, 2195
BseDI	CCNNGG	5 cut(s)	186, 755, 816, 1011, 1839
BseGI	GGATG	5 cut(s)	326, 550, 670, 1660, 2254
BseLI	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1776, 1948
BseMI	GCAATG	1 cut(s)	2223
BseMII	CTCAG	3 cut(s)	156, 221, 546
BseNI	ACTGG	3 cut(s)	959, 1706, 1902
BseRI	GAGGAG	1 cut(s)	88
BseSI	GKGCMC	1 cut(s)	1012
BseXI	GCAGC	9 cut(s)	121, 223, 504, 513, 550, 574, 1303, 1323, 1546
BseYI	CCCAGC	2 cut(s)	27, 130
BsgI	GTGCAG	1 cut(s)	549
Bsh1236I	CGCG	2 cut(s)	1866, 2087
BshFI	GGCC	2 cut(s)	250, 291
BsiHKAI	GWGCWC	1 cut(s)	1769
BsiHKCI	CYCGRG	1 cut(s)	464
BsiSI	CCGG	4 cut(s)	330, 447, 860, 1473
BslFI	GGGAC	2 cut(s)	1821, 2291
BslI	CCNNNNNNNGG	8 cut(s)	201, 369, 453, 556, 680, 1304, 1776, 1948
BsmAI	GTCTC	4 cut(s)	210, 1042, 1146, 1438
BsmFI	GGGAC	2 cut(s)	1821, 2291
BsmI	GAATGC	1 cut(s)	1255
BsnI	GGCC	2 cut(s)	250, 291
Bso31I	GGTCTC	1 cut(s)	210
BsoBI	CYCGRG	1 cut(s)	464
Bsp119I	TTCGAA	1 cut(s)	989
Bsp1286I	GDGCHC	4 cut(s)	131, 169, 1012, 1769
Bsp143I	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
Bsp1720I	GCTNAGC	1 cut(s)	142
Bsp68I	TCGCGA	1 cut(s)	1866
BspACI	CCGC	5 cut(s)	5, 237, 257, 389, 2087
BspANI	GGCC	2 cut(s)	250, 291
BspCNI	CTCAG	3 cut(s)	155, 220, 547
BspFNI	CGCG	2 cut(s)	1866, 2087
BspHI	TCATGA	1 cut(s)	1230
BspLI	GGNNCC	5 cut(s)	19, 1347, 1568, 1641, 2307
BspMAI	CTGCAG	1 cut(s)	592
BspOI	GCTAGC	3 cut(s)	141, 2232, 2300
BspPI	GGATC	4 cut(s)	380, 847, 1411, 1543
BspQI	GCTCTTC	2 cut(s)	501, 1757
BspT104I	TTCGAA	1 cut(s)	989
BspTNI	GGTCTC	1 cut(s)	210
BsrDI	GCAATG	1 cut(s)	2223
BsrFI	RCCGGY	2 cut(s)	446, 859
BsrI	ACTGG	3 cut(s)	959, 1706, 1902
BssAI	RCCGGY	2 cut(s)	446, 859
BssECI	CCNNGG	5 cut(s)	186, 755, 816, 1011, 1839
BssMI	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
BssNI	GRCGYC	1 cut(s)	303
BssT1I	CCWWGG	3 cut(s)	186, 816, 1839
Bst2UI	CCWGG	4 cut(s)	1013, 1510, 1533, 2195
Bst4CI	ACNGT	5 cut(s)	1672, 1729, 2011, 2064, 2238
Bst6I	CTCTTC	3 cut(s)	155, 501, 1757
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	517
BstBAI	YACGTR	2 cut(s)	265, 2018
BstBI	TTCGAA	1 cut(s)	989
BstC8I	GCNNGC	6 cut(s)	139, 448, 1689, 2184, 2230, 2298
BstDEI	CTNAG	3 cut(s)	142, 207, 555
BstDSI	CCRYGG	1 cut(s)	755
BstEII	GGTNACC	2 cut(s)	190, 1891
BstF5I	GGATG	5 cut(s)	326, 550, 670, 1660, 2254
BstFNI	CGCG	2 cut(s)	1866, 2087
BstH2I	RGCGCY	1 cut(s)	674
BstHHI	GCGC	2 cut(s)	673, 1033
BstKTI	GATC	7 cut(s)	280, 375, 842, 1240, 1406, 1441, 1538
BstMAI	GTCTC	4 cut(s)	210, 1042, 1146, 1438
BstMBI	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
BstMWI	GCNNNNNNNGC	7 cut(s)	143, 256, 265, 517, 523, 526, 1016
BstNI	CCWGG	4 cut(s)	1013, 1510, 1533, 2195
BstNSI	RCATGY	1 cut(s)	2186
BstPAI	GACNNNNGTC	2 cut(s)	188, 696
BstPI	GGTNACC	2 cut(s)	190, 1891
BstSCI	CCNGG	5 cut(s)	1011, 1471, 1508, 1531, 2193
BstSFI	CTRYAG	4 cut(s)	588, 687, 1149, 1431
BstSLI	GKGCMC	1 cut(s)	1012
BstUI	CGCG	2 cut(s)	1866, 2087
BstV1I	GCAGC	9 cut(s)	121, 223, 504, 513, 550, 574, 1303, 1323, 1546
BstV2I	GAAGAC	1 cut(s)	204
BstX2I	RGATCY	2 cut(s)	277, 372
BstXI	CCANNNNNNTGG	1 cut(s)	1909
BstYI	RGATCY	2 cut(s)	277, 372
Bsu36I	CCTNAGG	1 cut(s)	555
BsuRI	GGCC	2 cut(s)	250, 291
BtgI	CCRYGG	1 cut(s)	755
BtrI	CACGTC	1 cut(s)	229
BtsCI	GGATG	5 cut(s)	326, 550, 670, 1660, 2254
BtsI	GCAGTG	2 cut(s)	807, 832
BtsIMutI	CAGTG	6 cut(s)	215, 807, 832, 952, 1725, 1726
BtuMI	TCGCGA	1 cut(s)	1866
Cac8I	GCNNGC	6 cut(s)	139, 448, 1689, 2184, 2230, 2298
CaiI	CAGNNNCTG	2 cut(s)	217, 587
CciI	TCATGA	1 cut(s)	1230
CfoI	GCGC	2 cut(s)	673, 1033
Cfr10I	RCCGGY	2 cut(s)	446, 859
Cfr13I	GGNCC	5 cut(s)	327, 1259, 1566, 1640, 2305
CseI	GACGC	5 cut(s)	311, 686, 967, 1494, 2093
Csp6I	GTAC	1 cut(s)	178
CspCI	CAANNNNNGTGG	4 cut(s)	744, 779, 1606, 1641
CviAII	CATG	5 cut(s)	1197, 1231, 1620, 1651, 2183
CviQI	GTAC	1 cut(s)	178
DdeI	CTNAG	3 cut(s)	142, 207, 555
DpnI	GATC	7 cut(s)	279, 374, 841, 1239, 1405, 1440, 1537
DpnII	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
DriI	GACNNNNNGTC	1 cut(s)	727
EaeI	YGGCCR	1 cut(s)	289
Eam1104I	CTCTTC	3 cut(s)	155, 501, 1757
Eam1105I	GACNNNNNGTC	1 cut(s)	727
EarI	CTCTTC	3 cut(s)	155, 501, 1757
Ecl136II	GAGCTC	1 cut(s)	1767
Eco130I	CCWWGG	3 cut(s)	186, 816, 1839
Eco147I	AGGCCT	1 cut(s)	250
Eco24I	GRGCYC	3 cut(s)	131, 169, 1769
Eco31I	GGTCTC	1 cut(s)	210
Eco32I	GATATC	1 cut(s)	1920
Eco47I	GGWCC	5 cut(s)	327, 1259, 1566, 1640, 2305
Eco53kI	GAGCTC	1 cut(s)	1767
Eco57I	CTGAAG	5 cut(s)	471, 564, 1244, 1323, 1473
Eco72I	CACGTG	1 cut(s)	265
Eco81I	CCTNAGG	1 cut(s)	555
Eco88I	CYCGRG	1 cut(s)	464
Eco91I	GGTNACC	2 cut(s)	190, 1891
EcoICRI	GAGCTC	1 cut(s)	1767
EcoO109I	RGGNCCY	1 cut(s)	1566
EcoO65I	GGTNACC	2 cut(s)	190, 1891
EcoRI	GAATTC	2 cut(s)	404, 1632
EcoRII	CCWGG	4 cut(s)	1011, 1508, 1531, 2193
EcoRV	GATATC	1 cut(s)	1920
EcoT14I	CCWWGG	3 cut(s)	186, 816, 1839
EcoT22I	ATGCAT	1 cut(s)	2188
EcoT38I	GRGCYC	3 cut(s)	131, 169, 1769
ErhI	CCWWGG	3 cut(s)	186, 816, 1839
FaeI	CATG	5 cut(s)	1200, 1234, 1623, 1654, 2186
FalI	AAGNNNNNCTT	2 cut(s)	1671, 1703
FaqI	GGGAC	2 cut(s)	1821, 2291
FatI	CATG	5 cut(s)	1196, 1230, 1619, 1650, 2182
FauI	CCCGC	1 cut(s)	396
FblI	GTMKAC	1 cut(s)	300
FokI	GGATG	5 cut(s)	333, 537, 677, 1667, 2261
FriOI	GRGCYC	3 cut(s)	131, 169, 1769
FspBI	CTAG	9 cut(s)	138, 612, 834, 944, 1038, 1338, 1563, 2229, 2297
GlaI	GCGC	2 cut(s)	672, 1032
GsaI	CCCAGC	2 cut(s)	31, 134
GsuI	CTGGAG	1 cut(s)	1689
HaeII	RGCGCY	1 cut(s)	674
HaeIII	GGCC	2 cut(s)	250, 291
HapII	CCGG	4 cut(s)	330, 447, 860, 1473
HgaI	GACGC	5 cut(s)	311, 686, 967, 1494, 2093
HhaI	GCGC	2 cut(s)	673, 1033
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	5 cut(s)	1200, 1234, 1623, 1654, 2186
Hin6I	GCGC	2 cut(s)	671, 1031
HinP1I	GCGC	2 cut(s)	671, 1031
HincII	GTYRAC	1 cut(s)	301
HindII	GTYRAC	1 cut(s)	301
HindIII	AAGCTT	1 cut(s)	1685
HpaII	CCGG	4 cut(s)	330, 447, 860, 1473
HphI	GGTGA	4 cut(s)	184, 914, 950, 1903
Hpy166II	GTNNAC	3 cut(s)	301, 327, 2029
Hpy8I	GTNNAC	3 cut(s)	301, 327, 2029
Hpy99I	CGWCG	3 cut(s)	83, 233, 305
HpyAV	CCTTC	4 cut(s)	975, 985, 1064, 1298
HpyCH4III	ACNGT	5 cut(s)	1672, 1729, 2011, 2064, 2238
HpyCH4IV	ACGT	7 cut(s)	228, 264, 418, 885, 1800, 2017, 2278
HpyCH4V	TGCA	8 cut(s)	566, 590, 1253, 1465, 1877, 1930, 2148, 2186
HpyF10VI	GCNNNNNNNGC	7 cut(s)	143, 256, 265, 517, 523, 526, 1016
HpyF3I	CTNAG	3 cut(s)	142, 207, 555
HpySE526I	ACGT	7 cut(s)	228, 264, 418, 885, 1800, 2017, 2278
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	5 cut(s)	1200, 1234, 1623, 1654, 2186
HspAI	GCGC	2 cut(s)	671, 1031
KroI	GCCGGC	1 cut(s)	446
KroNI	GCCGGC	1 cut(s)	448
Kzo9I	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
LguI	GCTCTTC	2 cut(s)	501, 1757
LmnI	GCTCC	6 cut(s)	23, 75, 98, 634, 1772, 2104
Lsp1109I	GCAGC	9 cut(s)	121, 223, 504, 513, 550, 574, 1303, 1323, 1546
LweI	GCATC	8 cut(s)	520, 666, 1474, 1492, 1589, 2157, 2195, 2239
MaeI	CTAG	9 cut(s)	138, 612, 834, 944, 1038, 1338, 1563, 2229, 2297
MaeII	ACGT	7 cut(s)	228, 264, 418, 885, 1800, 2017, 2278
MaeIII	GTNAC	6 cut(s)	190, 748, 1721, 1891, 2005, 2219
MalI	GATC	7 cut(s)	279, 374, 841, 1239, 1405, 1440, 1537
MboI	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
MfeI	CAATTG	3 cut(s)	761, 1785, 1878
MflI	RGATCY	2 cut(s)	277, 372
MhlI	GDGCHC	4 cut(s)	131, 169, 1012, 1769
MlyI	GAGTC	5 cut(s)	420, 1163, 1395, 1933, 1981
MmeI	TCCRAC	2 cut(s)	570, 982
Mph1103I	ATGCAT	1 cut(s)	2188
MroNI	GCCGGC	1 cut(s)	446
MroXI	GAANNNNTTC	1 cut(s)	985
MseI	TTAA	8 cut(s)	713, 915, 1287, 1661, 1683, 1845, 2171, 2268
MslI	CAYNNNNRTG	1 cut(s)	1935
MspA1I	CMGCKG	3 cut(s)	134, 1187, 1523
MspI	CCGG	4 cut(s)	330, 447, 860, 1473
MspR9I	CCNGG	5 cut(s)	1013, 1473, 1510, 1533, 2195
MunI	CAATTG	3 cut(s)	761, 1785, 1878
Mva1269I	GAATGC	1 cut(s)	1255
MvaI	CCWGG	4 cut(s)	1013, 1510, 1533, 2195
MvnI	CGCG	2 cut(s)	1866, 2087
MwoI	GCNNNNNNNGC	7 cut(s)	143, 256, 265, 517, 523, 526, 1016
NaeI	GCCGGC	1 cut(s)	448
NciI	CCSGG	1 cut(s)	1473
NdeII	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
NgoMIV	GCCGGC	1 cut(s)	446
NheI	GCTAGC	3 cut(s)	137, 2228, 2296
NlaIII	CATG	5 cut(s)	1200, 1234, 1623, 1654, 2186
NlaIV	GGNNCC	5 cut(s)	19, 1347, 1568, 1641, 2307
NmuCI	GTSAC	4 cut(s)	190, 1721, 1891, 2219
NruI	TCGCGA	1 cut(s)	1866
NsiI	ATGCAT	1 cut(s)	2188
NspI	RCATGY	1 cut(s)	2186
NspV	TTCGAA	1 cut(s)	989
PaeI	GCATGC	1 cut(s)	2186
PagI	TCATGA	1 cut(s)	1230
PceI	AGGCCT	1 cut(s)	250
PciSI	GCTCTTC	2 cut(s)	501, 1757
PcsI	WCGNNNNNNNCGW	1 cut(s)	18
PctI	GAATGC	1 cut(s)	1255
PdiI	GCCGGC	1 cut(s)	448
PdmI	GAANNNNTTC	1 cut(s)	985
PfoI	TCCNGGA	2 cut(s)	1508, 1531
PleI	GAGTC	5 cut(s)	420, 1162, 1394, 1932, 1980
PmaCI	CACGTG	1 cut(s)	265
PmlI	CACGTG	1 cut(s)	265
PpsI	GAGTC	5 cut(s)	420, 1162, 1394, 1932, 1980
Ppu21I	YACGTR	2 cut(s)	265, 2018
PpuMI	RGGWCCY	1 cut(s)	1566
PshAI	GACNNNNGTC	2 cut(s)	188, 696
Psp124BI	GAGCTC	1 cut(s)	1769
Psp5II	RGGWCCY	1 cut(s)	1566
Psp6I	CCWGG	4 cut(s)	1011, 1508, 1531, 2193
PspCI	CACGTG	1 cut(s)	265
PspEI	GGTNACC	2 cut(s)	190, 1891
PspFI	CCCAGC	2 cut(s)	27, 130
PspGI	CCWGG	4 cut(s)	1011, 1508, 1531, 2193
PspN4I	GGNNCC	5 cut(s)	19, 1347, 1568, 1641, 2307
PspPI	GGNCC	5 cut(s)	327, 1259, 1566, 1640, 2305
PspPPI	RGGWCCY	1 cut(s)	1566
PstI	CTGCAG	1 cut(s)	592
PstNI	CAGNNNCTG	2 cut(s)	217, 587
PsuI	RGATCY	2 cut(s)	277, 372
PvuII	CAGCTG	3 cut(s)	134, 1187, 1523
RruI	TCGCGA	1 cut(s)	1866
RsaI	GTAC	1 cut(s)	179
RsaNI	GTAC	1 cut(s)	178
RseI	CAYNNNNRTG	1 cut(s)	1935
SacI	GAGCTC	1 cut(s)	1769
SalI	GTCGAC	1 cut(s)	299
SapI	GCTCTTC	2 cut(s)	501, 1757
SaqAI	TTAA	8 cut(s)	713, 915, 1287, 1661, 1683, 1845, 2171, 2268
Sau3AI	GATC	7 cut(s)	277, 372, 839, 1237, 1403, 1438, 1535
Sau96I	GGNCC	5 cut(s)	327, 1259, 1566, 1640, 2305
SchI	GAGTC	5 cut(s)	420, 1163, 1395, 1933, 1981
ScrFI	CCNGG	5 cut(s)	1013, 1473, 1510, 1533, 2195
SduI	GDGCHC	4 cut(s)	131, 169, 1012, 1769
SfaNI	GCATC	8 cut(s)	520, 666, 1474, 1492, 1589, 2157, 2195, 2239
SfcI	CTRYAG	4 cut(s)	588, 687, 1149, 1431
SfuI	TTCGAA	1 cut(s)	989
SinI	GGWCC	5 cut(s)	327, 1259, 1566, 1640, 2305
SmiMI	CAYNNNNRTG	1 cut(s)	1935
SmlI	CTYRAG	2 cut(s)	996, 2112
SmoI	CTYRAG	2 cut(s)	996, 2112
SpeI	ACTAGT	1 cut(s)	611
SphI	GCATGC	1 cut(s)	2186
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	5 cut(s)	5, 237, 257, 389, 2087
SspMI	CTAG	9 cut(s)	138, 612, 834, 944, 1038, 1338, 1563, 2229, 2297
SstI	GAGCTC	1 cut(s)	1769
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	5 cut(s)	1011, 1471, 1508, 1531, 2193
StyI	CCWWGG	3 cut(s)	186, 816, 1839
TaaI	ACNGT	5 cut(s)	1672, 1729, 2011, 2064, 2238
TaiI	ACGT	7 cut(s)	231, 267, 421, 888, 1803, 2020, 2281
TaqI	TCGA	8 cut(s)	12, 231, 300, 408, 989, 1922, 2067, 2151
TatI	WGTACW	1 cut(s)	177
TauI	GCSGC	1 cut(s)	259
Tru1I	TTAA	8 cut(s)	713, 915, 1287, 1661, 1683, 1845, 2171, 2268
Tru9I	TTAA	8 cut(s)	713, 915, 1287, 1661, 1683, 1845, 2171, 2268
TscAI	CASTG	6 cut(s)	222, 814, 832, 959, 1726, 1732
TseFI	GTSAC	4 cut(s)	190, 1721, 1891, 2219
TseI	GCWGC	9 cut(s)	134, 211, 517, 526, 563, 587, 1291, 1311, 1559
Tsp45I	GTSAC	4 cut(s)	190, 1721, 1891, 2219
TspDTI	ATGAA	6 cut(s)	1170, 1467, 1569, 1671, 2060, 2274
TspGWI	ACGGA	3 cut(s)	333, 1295, 1705
TspRI	CASTG	6 cut(s)	222, 814, 832, 959, 1726, 1732
VpaK11BI	GGWCC	5 cut(s)	327, 1259, 1566, 1640, 2305
XapI	RAATTY	2 cut(s)	404, 1632
XceI	RCATGY	1 cut(s)	2186
XmiI	GTMKAC	1 cut(s)	300
XmnI	GAANNNNTTC	1 cut(s)	985
XspI	CTAG	9 cut(s)	138, 612, 834, 944, 1038, 1338, 1563, 2229, 2297
Zsp2I	ATGCAT	1 cut(s)	2188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.