Rorug03G0230000

Protein of unknown function (DUF724)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004216
Physical Location & Seq
Forward (+)
21115555 .. 21118689
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0230000.1

Sequence Viewer

Length: 3135 bp
ATGCCAACTCTGTTCCTTCATTTCTTCCTTCTCTTATTCACTACCACTGTTATCCCCGCAGTTCACAGCAACTGTATTGAAGCCCAGAAACAATCATTGCTTCATTTCAAACTAAGTCTTGTACCTTATTCTTCCCCTTCCTCCAAGCTTATCACATGGAATTCGAGTACCGACTGCTGTTCTTGGCTCGGTATAACTTGCAGCACCAATGGGCGTGTTGTCGAACTTGACCTCAGCGGAGAGTATTTCTCATGCAACATTGACAATTCAAGCAGTCTCTTCCAACTTCAACATCTTCAGAGCCTCAATTTGGCCGAGAATTACTTCAATGACTCTTCAATTCCATCCGCAATTGGAAAGCTTACAGAGCTGAGGTATCTAAATTTAAGTGATGCTTTTTTTTCTGGGGTTATTCCCATTGAGATTTCACGCTTAACAAGACTGATAACTCTTGATCTCTCTGGTAATTACGGCCCCTCTGTTGCTGAGAACCAGAATTTAAGCATGCTAATTCAGAACCTCACAGAGCTTACAGAGTTATATCTTGATTATGTAGAGATATCAGGACAGGGGAGTGACTGGTGCGAAGCCATATCATCTTCACTGCCAAATCTGAGGGTGTTGAGCTTGTCTTTTTGTGGTCTTTCAGGCCCTATATGTGACTCCCTTGCAAAGCTTCAATTTCTGTATGAGATTGACTTGTCATATAACTCCTTCTATGCCCCGGTTCCAAGAGTCTTTGCCAATTTTTCAAACTTGAGTTACTTGAGTCTTCGTTATTCGAAATTGCTCGGAACATTTCCCAAAGAGGTCTTTCAGATACCTTCCCTACAAACCATTGACCTTTCTGCTAATTATGCTCTTCATGCTTCCTTTCCAGAATTTCCAAACAATGGGTCTCTTCGATCCTTAATTGTATCTGGGACAAATTTTTCAGGTGACCTGCCCAACTCTATTCGAAATCTTAAGATGTTGTCAAGCATAGATATTTCAAATTGCAATTTCACTGGATCAGTTCCCAAGTCGATTGAAAACCTCACACTATTGGCTGACCTGGACATGTCAGGGAACAGATTTAATAGTCCCATTAGTTCTTTTCACTGGGAAACCCTTGTTAATCTGGAAATCCTCGATTTGAGCAACAATCAACTCTATGGGACTATTCCTTCATCTGTTGTTTCTCTTCCCAAACTGGAAACAGTAAAACTTTCCCACAATCGGTTCTCGGTTATAGAAGCTCCTCTTCCTAATTCCACTTCTGCTTTATCTTCTTTGGACATTCATTCTAACAAGCTTCAAGGCGAGATACCTAATTGGATTTTGAGTCTCAAATCTCTTAATTCCCTTGATCTTTCTAACAACTCCTTGGTGACTCTAGAAGCTCCTCTTCCTAATTCCACTTCTGCTTTATCTTCTCTGGACCTTCATTCCAACAAACTTCAAGGTATCCATTCCTCATATTCCTTCCTTCAGCTTAATCACTTGGGATTATCATTAGCTTCTAACAAGTTGACAAGATTCCCAGACTTCTTGAGAAATCAATCCCATTTAACCAACTTGGACCTCTCAGACAACCAGATTCAAGGACAGATACCCAATTGGATTTGGATTTTGGATTCTCTTCAAAAACTAAATCTTTCTTGTAACTCCTTGGAAAGTTTTGAAGCTCCTCTACCTAATTCTACCTATGTTCTGTCAACGGTTGATCTTCATTCAAACCAGCTTCAGGGACATTTCCCCTTGTTCTTCAGTAGTAGGTATCTAGATTATTCAAGAAATAATTTCAGCTCTAGTATACCGACTGACATTGGTGACTTCATTTCTTCTGGGTCTTACCTTTCTCTTTCAAGCAATAACTTCCACGGGGCCATTCCAAAATCAATATGCAATGCAATTGGTCGGGTTCTTGATCTGTCCAATAATTCCTTCAGTGGAAACATTCCCCAATGCTTGACTCAATTAGATCTGCTTTCGGTCATTGATTTGAAGAGAAACAACCTGAGTGGCACAATTCCTGATAATTTTCTCAACAGTTGTGACCTACAAACTCTAGACCTCAGTAACAATCAGATACAAGGCCGGTTTCCGAAATCTCTTGTCAACTGCTCAAGGTTAGAGGTTTTAAACCTTGGAAACAATAATATAACTGATACTTTTCCATGCTTTCTAATGAGCTTATCCACCTTACGTATCCTTGTTTTGCGGTCCAACAAGTTTTATGGATGCATTGGATGTCCCAAGACCAATGACACCTGGCCTGTGCTTCAAATAATAGATTTAGCTCACAACAATTTCAGTGGTGATATGCCAGGAAGATTGTCTCTGACATGGCAGGCTATGGTGTCTAATGAGGATGATTCTCCAACTAACCTTGGATTTTCTTATCAGCCGTGCAGGAATAGTTCAATTGATATATGTTATTTGAATAATTTACAAAGAGATTCAGTTTATTATAGAGATGCAATTATGACTATCATCAAAGGTTTAGAGTTGGATGTAGTTAAGATTTTAACTATATTCACCTTGATTGACTACTCCTCCAACAAATTCAACGGATCAATACCTGAGGAAATCGGAGAGTTGAAATCACTATATTTCCTCAATTTATCCGGTAATGCTTTCACCGGAAACATCCCATCATCATTTGGTAAGATGGGGCAATTGGAGTCCTTAGACCTCTCACAGAACCACTTGAGCGGCCAAATTCCCCCCGAGTTTGCAAATCTCAATTTCCTTTCGCACTTGAATGTCTCATATAATCAATTGACGGGAAGGATCCCAACCAACACTCAGTTTTCGACATTTCCAAGTACCTCCTTCGAGGGTAATAAAGGATTGTGGGGGCCTCCTCTGACATCGGATACAGCAATCGTATTGCCACCTCCAAAGTTTAATGGAAGCTCAAGTAATCCGAATTCTGGAGATGAGATTGATTGGAATTTTATCAGTGCTGAAATTGGATTTACATGTGGGTTTGGAATTGCCGTCGGGTCACTTTTGTTTTGCAAGAGATGGAGGAAATGGTATTACAGAGCTATGTTTAAGATCCTGTTCAAGATATTCCCTCAGCTGGAACACAGATTTGGTAATCACAGAAGGCATGTTTACATAAATCAAAAGTATTGGAGACGTTGA

Protein Analysis

1044

Amino Acids

116.31

Weight (kDa)

5.74

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 5.1e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 82 - 231 5.3e-10 Leucine-rich repeat region
LRR_8 PF13855 229 - 285 4.9e-06 Leucine rich repeat
LRR_8 PF13855 396 - 456 4.1e-08 Leucine rich repeat
LRR_8 PF13855 681 - 739 2.1e-08 Leucine rich repeat
LRR_4 PF12799 681 - 717 4e-07 Leucine Rich repeats (2 copies)
LRR_14 PF23598 853 - 917 9.5e-06 Leucine-rich repeat region
LRR_8 PF13855 863 - 922 3e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 951
AccB7I CCANNNNNTGG 1 cut(s) 893
AccBSI CCGCTC 1 cut(s) 2697
AccI GTMKAC 1 cut(s) 1795
AciI CCGC 5 cut(s) 57, 237, 348, 2203, 2697
AclWI GGATC 6 cut(s) 900, 1018, 2563, 2770, 2783, 3040
AcoI YGGCCR 2 cut(s) 312, 2698
AcsI RAATTY 9 cut(s) 160, 382, 496, 881, 928, 2546, 2703, 2914, 2938
AcuI CTGAAG 5 cut(s) 281, 1454, 1709, 1732, 1912
AfaI GTAC 3 cut(s) 123, 169, 2812
AfiI CCNNNNNNNGG 6 cut(s) 310, 893, 1218, 1726, 2918, 3070
AflII CTTAAG 1 cut(s) 965
AflIII ACRYGT 2 cut(s) 1059, 2966
AjnI CCWGG 3 cut(s) 1053, 2252, 2308
AjuI GAANNNNNNNTTGG 4 cut(s) 1910, 1942, 3066, 3098
Alw26I GTCTC 6 cut(s) 281, 903, 1331, 2325, 2755, 3121
AlwI GGATC 6 cut(s) 900, 1018, 2563, 2770, 2783, 3040
AlwNI CAGNNNCTG 1 cut(s) 72
Ama87I CYCGRG 1 cut(s) 2711
AoxI GGCC 8 cut(s) 312, 472, 649, 1866, 2077, 2255, 2698, 2843
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 9 cut(s) 160, 382, 496, 881, 928, 2546, 2703, 2914, 2938
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
Asp700I GAANNNNTTC 1 cut(s) 323
AspS9I GGNCC 7 cut(s) 473, 650, 1420, 1561, 1866, 2205, 2843
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 6 cut(s) 950, 1381, 1823, 2312, 2512, 2614
AsuII TTCGAA 2 cut(s) 782, 958
AvaI CYCGRG 1 cut(s) 2711
AvaII GGWCC 3 cut(s) 1420, 1561, 2205
AxyI CCTNAGG 1 cut(s) 2565
BamHI GGATCC 1 cut(s) 2775
BarI GAAGNNNNNNTAC 2 cut(s) 1656, 1688
BbsI GAAGAC 1 cut(s) 764
BbvCI CCTCAGC 3 cut(s) 233, 371, 3066
BbvI GCAGC 1 cut(s) 213
BccI CCATC 4 cut(s) 352, 2644, 2647, 3006
BceAI ACGGC 3 cut(s) 487, 2374, 2969
BciT130I CCWGG 3 cut(s) 1055, 2254, 2310
BciVI GTATCC 3 cut(s) 1457, 2201, 2854
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 6 cut(s) 281, 903, 1331, 2325, 2755, 3121
BfaI CTAG 4 cut(s) 1376, 1763, 1791, 2051
BfrI CTTAAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 951
BfuI GTATCC 3 cut(s) 1457, 2201, 2854
BglII AGATCT 1 cut(s) 1963
BisI GCNGC 2 cut(s) 202, 2698
BlsI GCNGC 2 cut(s) 203, 2699
Bme1390I CCNGG 4 cut(s) 725, 1055, 2254, 2310
Bme18I GGWCC 3 cut(s) 1420, 1561, 2205
BmeT110I CYCGRG 1 cut(s) 2711
BmgT120I GGNCC 7 cut(s) 473, 650, 1420, 1561, 1866, 2205, 2843
BmiI GGNNCC 5 cut(s) 475, 729, 1867, 2777, 2844
BmrFI CCNGG 4 cut(s) 725, 1055, 2254, 2310
BmrI ACTGGG 1 cut(s) 1111
BmsI GCATC 3 cut(s) 382, 2213, 2449
BmuI ACTGGG 1 cut(s) 1111
BpiI GAAGAC 1 cut(s) 764
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 1 cut(s) 2940
Bpu10I CCTNAGC 3 cut(s) 233, 371, 3066
Bpu14I TTCGAA 2 cut(s) 782, 958
BpuEI CTTGAG 6 cut(s) 778, 787, 1552, 2092, 2713, 2887
BpuMI CCSGG 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 2189
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 6 cut(s) 723, 1365, 1650, 1861, 2128, 2371
BsaWI WCCGGW 2 cut(s) 2609, 2624
BsaXI ACNNNNNCTCC 2 cut(s) 2522, 2552
Bsc4I CCNNNNNNNGG 6 cut(s) 310, 893, 1218, 1726, 2918, 3070
Bse118I RCCGGY 1 cut(s) 2079
Bse1I ACTGG 4 cut(s) 584, 1012, 1106, 1197
Bse21I CCTNAGG 1 cut(s) 2565
Bse3DI GCAATG 2 cut(s) 95, 1894
BseBI CCWGG 3 cut(s) 1055, 2254, 2310
BseDI CCNNGG 6 cut(s) 723, 1365, 1650, 1861, 2128, 2371
BseGI GGATG 6 cut(s) 344, 2228, 2237, 2359, 2500, 2631
BseLI CCNNNNNNNGG 6 cut(s) 310, 893, 1218, 1726, 2918, 3070
BseMI GCAATG 2 cut(s) 95, 1894
BseNI ACTGG 4 cut(s) 584, 1012, 1106, 1197
BseRI GAGGAG 5 cut(s) 1230, 1374, 1659, 2527, 2838
BseXI GCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 2413
BshFI GGCC 8 cut(s) 314, 474, 651, 1868, 2079, 2257, 2700, 2845
BsiHKCI CYCGRG 1 cut(s) 2711
BsiSI CCGG 4 cut(s) 725, 2080, 2610, 2625
BslFI GGGAC 5 cut(s) 937, 1068, 1171, 1743, 2220
BslI CCNNNNNNNGG 6 cut(s) 310, 893, 1218, 1726, 2918, 3070
BsmAI GTCTC 6 cut(s) 281, 903, 1331, 2325, 2755, 3121
BsmBI CGTCTC 1 cut(s) 3121
BsmFI GGGAC 5 cut(s) 937, 1068, 1171, 1743, 2220
BsnI GGCC 8 cut(s) 314, 474, 651, 1868, 2079, 2257, 2700, 2845
Bso31I GGTCTC 1 cut(s) 903
BsoBI CYCGRG 1 cut(s) 2711
Bsp119I TTCGAA 2 cut(s) 782, 958
BspACI CCGC 5 cut(s) 57, 237, 348, 2203, 2697
BspANI GGCC 8 cut(s) 314, 474, 651, 1868, 2079, 2257, 2700, 2845
BspLI GGNNCC 5 cut(s) 475, 729, 1867, 2777, 2844
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 6 cut(s) 900, 1018, 2563, 2770, 2783, 3040
BspQI GCTCTTC 1 cut(s) 867
BspT104I TTCGAA 2 cut(s) 782, 958
BspTI CTTAAG 1 cut(s) 965
BspTNI GGTCTC 1 cut(s) 903
BsrBI CCGCTC 1 cut(s) 2697
BsrDI GCAATG 2 cut(s) 95, 1894
BsrFI RCCGGY 1 cut(s) 2079
BsrI ACTGG 4 cut(s) 584, 1012, 1106, 1197
BssAI RCCGGY 1 cut(s) 2079
BssECI CCNNGG 6 cut(s) 723, 1365, 1650, 1861, 2128, 2371
BssNAI GTATAC 1 cut(s) 1796
BssT1I CCWWGG 4 cut(s) 1365, 1650, 2128, 2371
Bst1107I GTATAC 1 cut(s) 1796
Bst2UI CCWGG 3 cut(s) 1055, 2254, 2310
Bst4CI ACNGT 5 cut(s) 49, 74, 1201, 1702, 2033
Bst6I CTCTTC 9 cut(s) 284, 340, 867, 906, 1188, 1248, 1392, 1626, 1982
BstAFI CTTAAG 1 cut(s) 965
BstBAI YACGTR 1 cut(s) 2189
BstBI TTCGAA 2 cut(s) 782, 958
BstC8I GCNNGC 2 cut(s) 506, 2334
BstDSI CCRYGG 1 cut(s) 1861
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 6 cut(s) 344, 2228, 2237, 2359, 2500, 2631
BstMAI GTCTC 6 cut(s) 281, 903, 1331, 2325, 2755, 3121
BstMWI GCNNNNNNNGC 3 cut(s) 367, 857, 866
BstNI CCWGG 3 cut(s) 1055, 2254, 2310
BstNSI RCATGY 4 cut(s) 508, 1063, 2970, 3104
BstPI GGTNACC 1 cut(s) 938
BstSCI CCNGG 4 cut(s) 723, 1053, 2252, 2308
BstSNI TACGTA 1 cut(s) 2189
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 764
BstX2I RGATCY 3 cut(s) 1963, 2775, 3045
BstYI RGATCY 3 cut(s) 1963, 2775, 3045
BstZ17I GTATAC 1 cut(s) 1796
Bsu36I CCTNAGG 1 cut(s) 2565
BsuI GTATCC 3 cut(s) 1457, 2201, 2854
BsuRI GGCC 8 cut(s) 314, 474, 651, 1868, 2079, 2257, 2700, 2845
BtgI CCRYGG 1 cut(s) 1861
BtsCI GGATG 6 cut(s) 344, 2228, 2237, 2359, 2500, 2631
BtsI GCAGTG 1 cut(s) 602
BtsIMutI CAGTG 7 cut(s) 45, 602, 1005, 1099, 1936, 2302, 2953
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 2 cut(s) 506, 2334
CaiI CAGNNNCTG 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 2079
Cfr13I GGNCC 7 cut(s) 473, 650, 1420, 1561, 1866, 2205, 2843
Csp6I GTAC 3 cut(s) 122, 168, 2811
CspCI CAANNNNNGTGG 4 cut(s) 1984, 2019, 2278, 2313
CviAII CATG 9 cut(s) 156, 252, 505, 866, 1060, 2160, 2328, 2967, 3101
CviQI GTAC 3 cut(s) 122, 168, 2811
DraI TTTAAA 1 cut(s) 2124
EaeI YGGCCR 2 cut(s) 312, 2698
Eam1104I CTCTTC 9 cut(s) 284, 340, 867, 906, 1188, 1248, 1392, 1626, 1982
EarI CTCTTC 9 cut(s) 284, 340, 867, 906, 1188, 1248, 1392, 1626, 1982
Eco105I TACGTA 1 cut(s) 2189
Eco130I CCWWGG 4 cut(s) 1365, 1650, 2128, 2371
Eco31I GGTCTC 1 cut(s) 903
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 3 cut(s) 1420, 1561, 2205
Eco57I CTGAAG 5 cut(s) 281, 1454, 1709, 1732, 1912
Eco81I CCTNAGG 1 cut(s) 2565
Eco88I CYCGRG 1 cut(s) 2711
Eco91I GGTNACC 1 cut(s) 938
EcoO109I RGGNCCY 2 cut(s) 650, 2843
EcoO65I GGTNACC 1 cut(s) 938
EcoRI GAATTC 2 cut(s) 160, 2914
EcoRII CCWGG 3 cut(s) 1053, 2252, 2308
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 4 cut(s) 1365, 1650, 2128, 2371
EcoT22I ATGCAT 1 cut(s) 2228
ErhI CCWWGG 4 cut(s) 1365, 1650, 2128, 2371
Esp3I CGTCTC 1 cut(s) 3121
FaeI CATG 9 cut(s) 159, 255, 508, 869, 1063, 2163, 2331, 2970, 3104
FalI AAGNNNNNCTT 6 cut(s) 379, 411, 1227, 1259, 1371, 1403
FaqI GGGAC 5 cut(s) 937, 1068, 1171, 1743, 2220
FatI CATG 9 cut(s) 155, 251, 504, 865, 1059, 2159, 2327, 2966, 3100
FauI CCCGC 1 cut(s) 64
FblI GTMKAC 1 cut(s) 1795
Fnu4HI GCNGC 2 cut(s) 202, 2698
FokI GGATG 6 cut(s) 331, 2235, 2244, 2366, 2507, 2618
Fsp4HI GCNGC 2 cut(s) 202, 2698
FspBI CTAG 4 cut(s) 1376, 1763, 1791, 2051
GluI GCNGC 2 cut(s) 202, 2698
GsuI CTGGAG 1 cut(s) 2940
HaeIII GGCC 8 cut(s) 314, 474, 651, 1868, 2079, 2257, 2700, 2845
HapII CCGG 4 cut(s) 725, 2080, 2610, 2625
Hin1II CATG 9 cut(s) 159, 255, 508, 869, 1063, 2163, 2331, 2970, 3104
HincII GTYRAC 3 cut(s) 1512, 1698, 2101
HindII GTYRAC 3 cut(s) 1512, 1698, 2101
HindIII AAGCTT 4 cut(s) 146, 359, 674, 1292
HpaII CCGG 4 cut(s) 725, 2080, 2610, 2625
HphI GGTGA 6 cut(s) 950, 1381, 1823, 2312, 2512, 2614
Hpy166II GTNNAC 6 cut(s) 64, 1512, 1698, 1796, 2101, 3106
Hpy8I GTNNAC 6 cut(s) 64, 1512, 1698, 1796, 2101, 3106
Hpy99I CGWCG 1 cut(s) 2990
HpyCH4III ACNGT 5 cut(s) 49, 74, 1201, 1702, 2033
HpyCH4IV ACGT 2 cut(s) 2188, 3130
HpyF10VI GCNNNNNNNGC 3 cut(s) 367, 857, 866
HpySE526I ACGT 2 cut(s) 2188, 3130
Hsp92II CATG 9 cut(s) 159, 255, 508, 869, 1063, 2163, 2331, 2970, 3104
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 3 cut(s) 1243, 1387, 1672
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 3 cut(s) 382, 2213, 2449
MaeI CTAG 4 cut(s) 1376, 1763, 1791, 2051
MaeII ACGT 2 cut(s) 2188, 3130
MbiI CCGCTC 1 cut(s) 2697
MfeI CAATTG 6 cut(s) 351, 1597, 1893, 2406, 2660, 2762
MflI RGATCY 3 cut(s) 1963, 2775, 3045
MlyI GAGTC 8 cut(s) 326, 656, 744, 778, 1333, 1366, 1948, 2675
MmeI TCCRAC 6 cut(s) 307, 1455, 2232, 2387, 2472, 2565
Mph1103I ATGCAT 1 cut(s) 2228
MroXI GAANNNNTTC 1 cut(s) 323
MslI CAYNNNNRTG 1 cut(s) 2745
MspA1I CMGCKG 2 cut(s) 237, 3070
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 4 cut(s) 725, 2080, 2610, 2625
MspR9I CCNGG 4 cut(s) 725, 1055, 2254, 2310
MunI CAATTG 6 cut(s) 351, 1597, 1893, 2406, 2660, 2762
MvaI CCWGG 3 cut(s) 1055, 2254, 2310
MwoI GCNNNNNNNGC 3 cut(s) 367, 857, 866
NciI CCSGG 1 cut(s) 725
NlaIII CATG 9 cut(s) 159, 255, 508, 869, 1063, 2163, 2331, 2970, 3104
NlaIV GGNNCC 5 cut(s) 475, 729, 1867, 2777, 2844
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 7 cut(s) 575, 659, 938, 1369, 1811, 2036, 2991
NsiI ATGCAT 1 cut(s) 2228
NspI RCATGY 4 cut(s) 508, 1063, 2970, 3104
NspV TTCGAA 2 cut(s) 782, 958
PaeI GCATGC 1 cut(s) 508
PciI ACATGT 2 cut(s) 1059, 2966
PciSI GCTCTTC 1 cut(s) 867
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 5 cut(s) 1518, 1579, 1616, 2357, 2441
PflMI CCANNNNNTGG 1 cut(s) 893
PkrI GCNGC 2 cut(s) 203, 2699
PleI GAGTC 8 cut(s) 326, 656, 743, 777, 1332, 1366, 1948, 2674
PpsI GAGTC 8 cut(s) 326, 656, 743, 777, 1332, 1366, 1948, 2674
Ppu21I YACGTR 1 cut(s) 2189
PscI ACATGT 2 cut(s) 1059, 2966
Psp6I CCWGG 3 cut(s) 1053, 2252, 2308
PspEI GGTNACC 1 cut(s) 938
PspGI CCWGG 3 cut(s) 1053, 2252, 2308
PspN4I GGNNCC 5 cut(s) 475, 729, 1867, 2777, 2844
PspPI GGNCC 7 cut(s) 473, 650, 1420, 1561, 1866, 2205, 2843
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 3 cut(s) 1963, 2775, 3045
PvuII CAGCTG 1 cut(s) 3070
RsaI GTAC 3 cut(s) 123, 169, 2812
RsaNI GTAC 3 cut(s) 122, 168, 2811
RseI CAYNNNNRTG 1 cut(s) 2745
SapI GCTCTTC 1 cut(s) 867
SatI GCNGC 2 cut(s) 202, 2698
Sau96I GGNCC 7 cut(s) 473, 650, 1420, 1561, 1866, 2205, 2843
SchI GAGTC 8 cut(s) 326, 656, 744, 778, 1333, 1366, 1948, 2675
ScrFI CCNGG 4 cut(s) 725, 1055, 2254, 2310
SfaNI GCATC 3 cut(s) 382, 2213, 2449
SfuI TTCGAA 2 cut(s) 782, 958
SinI GGWCC 3 cut(s) 1420, 1561, 2205
SmiMI CAYNNNNRTG 1 cut(s) 2745
SmlI CTYRAG 7 cut(s) 757, 766, 965, 1531, 2107, 2692, 2902
SmoI CTYRAG 7 cut(s) 757, 766, 965, 1531, 2107, 2692, 2902
SnaBI TACGTA 1 cut(s) 2189
SphI GCATGC 1 cut(s) 508
SsiI CCGC 5 cut(s) 57, 237, 348, 2203, 2697
SspMI CTAG 4 cut(s) 1376, 1763, 1791, 2051
StyD4I CCNGG 4 cut(s) 723, 1053, 2252, 2308
StyI CCWWGG 4 cut(s) 1365, 1650, 2128, 2371
TaaI ACNGT 5 cut(s) 49, 74, 1201, 1702, 2033
TaiI ACGT 2 cut(s) 2191, 3133
TaqI TCGA 9 cut(s) 164, 222, 782, 904, 958, 1025, 1131, 2798, 2820
TaqII GACCGA 1 cut(s) 1963
TauI GCSGC 1 cut(s) 2700
TfiI GAWTC 5 cut(s) 1518, 1579, 1616, 2357, 2441
TscAI CASTG 7 cut(s) 52, 609, 1012, 1106, 1936, 2302, 2953
TseFI GTSAC 7 cut(s) 575, 659, 938, 1369, 1811, 2036, 2991
TseI GCWGC 1 cut(s) 201
Tsp45I GTSAC 7 cut(s) 575, 659, 938, 1369, 1811, 2036, 2991
TspDTI ATGAA 8 cut(s) 8, 92, 854, 1158, 1271, 1415, 1700, 1807
TspGWI ACGGA 1 cut(s) 2568
TspRI CASTG 7 cut(s) 52, 609, 1012, 1106, 1936, 2302, 2953
Van91I CCANNNNNTGG 1 cut(s) 893
Vha464I CTTAAG 1 cut(s) 965
VpaK11BI GGWCC 3 cut(s) 1420, 1561, 2205
XapI RAATTY 9 cut(s) 160, 382, 496, 881, 928, 2546, 2703, 2914, 2938
XbaI TCTAGA 3 cut(s) 1375, 1762, 2050
XceI RCATGY 4 cut(s) 508, 1063, 2970, 3104
XmiI GTMKAC 1 cut(s) 1795
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 4 cut(s) 1376, 1763, 1791, 2051
Zsp2I ATGCAT 1 cut(s) 2228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.