Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004216

Physical Location & Seq

Genomic Coordinates Reverse (-)

33861072 .. 33867226

Gene Structure 6155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1278800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGGCGGGCTCGGAGGGGAGAGAAGAAGAGCAGCAGCAGCTCCAATTGTTCGGCGTGGGCTCAGAAGTAGAAGTGAGGACCGACGAGGAAGGGTTCAAAGGCGCCTTGTTCAGAGCCACCATTGTCACAAGCCCCACAAACTCCGCCTCAAAGAAGAGGAAAAGGGCGTTGGTCGAGTACAAGAACTTGGTCACGGAAGACGGGTCTAAGCAGCTCAAGGAGTACGTCGATTCGGAGCACCTGAGGCCCACGCCGCCGCAGCTCGCTGACCGGAATTTTGAGGAGGGAGATGTGGTGGATGCGGATTACAGAGATGGGTGGTGGACTGGGGTCGTAGGGAAGGTTGTCGAGAACAATTCCAAGTACAGCGTCGTCTTCGACAACCCTCCGGATTTGATCGAATTTCAGAAAGACCGTCTCAGATTGCATCAAGATTGGGTTAATGGCGAATGGGTTCGGCCCAATAAGCAGGAAGTATTGGCTGCTTCAGATACTCCACAAGAATTAGAGCATTTGTTGCCTGCTCAAAAAGATTCTAACAATCTTGAAGTGGCTACTAAACTAGAAAATTTGAGTGCTGCAGAGGAGAATCCTGAATCAACCAACTCAGGGAAGAATTTAATGGAGCAGCCAAGTTATCCACGAAGCGTCAAGGACAAAAAGATGTTAACCCGTAATGGTAGTGCTACAGACTCGCGTCCGGTTAAAAAGTTGAAAGACGACAAAGCAGCTGAGCCCATATTATCCATTACAGCACGTCAATTGAGGAAAACGCCTGACAATAAAGAAATACCCCAGGAATTACCTCAACTGAGCACAGGAGTTAGGGGAACAAGACGCACACGGAAACCTGTTGTACGCCATCAGTTTATTAAAACAGAGAGCCTGCTCGGGAAAAATAATGTTAAGACTAAGCAAGAGAATGATGGTGAAGTGAATAGCCAGTGGATTCATCCCGTAGCAAGTAAAGGAAGGCGCACAAAATCTCAAACTGGAAGTCGATTGATCCCAGAAGCAGGGTATGTCTGTGCCCTGCCTAGCCAAAAGAGAGAGAAAGAAAAGGATAAGGAAGCGTCTGCAAGTGTTTCTTTGGCTGGACAAAATGTTCAGAATGAAGGTAATATTAAAGAAACTGAAGTGCCTCAAACTATCTGGTCAACAACTAAGGGCAAGGAAGTTACACTGGCTGAAAAACCGGCTCAGCTACCTGATCAAGAGTTGCAAGTGAAAGATCAGAAAAAGAGTGCAAATGATCCTGCAAAACCAGCTCAGCTACCTGATCAAGAGTTGCAGGTGAAGGATCAGAAGAAGAGTGCAAATGATCCTGCAAATGAAAAGAGCATGGTTATGTCAGCTGAACTTGGCAGTGATTCAATTTTGGCTAACCTGCTCCGTAGCCTGGTTACTTTTCCAGACATGGAGTTTAAGCAGCAACAAGCTGGAGGAAGTAGTCATAAAAGAAAGAGAGGCAGGCCTCGAAAGTTAGCGGTTGTAAGGTCACGAGCTTCAGACGGAGTTAAGGGGCAGAATATGTCAGGAAATGTTGCTGACAAAAATGATAAGAATGATCAGACGCCTGAGGAAGCAGCTTTGCATGTGCTGAGGGGGATGGATTCTACAGCTGCAAAAGATGCCTCTAAAAGAAAAACAGCCGATTTTCCTGGAAGGTGCAAAATAAAAGAAGCTGCACCAATAGCATCTGATAATCCAGATGATGATGATAAACCTCTATCCATGTGGTTTGGGGGGATGCAGCTTTCTGCGTATGTTGGTGAATCAAGACCATCCCCTGATGCAAATGTCAACCAGCATAATGATAGACAAGAACCAGTTGAGGTAGCTAGCGAATCTCCTGCAGTTGATGCAATCAGTGGCAGCGGGCCATATGAGGAGCAAGGGTTGCCTTTTGTTAAGAGCTCCCCTGTGTGGAAGACAATTGAAACATTGGAAGTATTCAGGCTTTTCCCCCAAAATCCTCATTTCCGACCTCTGGTTGAATGCAAAGAGGAATACCGCGAGGGTTCAGCAATTGGAAACATGATAACCTTTTCCAGTCTGACGGATAAAATATCCAGGCTGCAGTTCGACGACCATCAAAGTGTTTTTGATAGCATTTTGGAGAGTCTTGTTGACTTGGAAAATTATGGATTCAATGTTACAATTCTACGCAGGCGGGTGAATGATCTGCTGTCTGTTAAGGACAAGCAAGGGCGGTTTCAGGTCGAGTCAAGGGATGCTGAACAGAAGATCATGGAGCATTCTCGTGAGAAGAACAAACTTGTCGAAGAGGCTGACTATATTGCGAAGAAAATAATTGAGTTGCAGGACAAACATGCATCAATCAAGTCAGAAGTGGAGGCCAAAGAACATGTGATTGCTAGATTGCGAATGTCTTTTGATGCCATGAATGAAGGCATTCAGAGCGCTCGGAGTGATTTTGAAAAGCTAGCTTTGGCTCCCATGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046983

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Agenet DUF724

Protein Analysis

823

Amino Acids

91.95

Weight (kDa)

6.15

Isoelectric Point (pI)

48.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Agenet	PF05641	20 - 86	7.8e-17	Agenet domain
DUF724	PF05266	634 - 820	5.9e-62	Protein of unknown function (DUF724)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004216)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g26800 FvH4_1g26800 FvH4_1g26800 FvH4_1g26800
malus_domestica	MD09G1286400.v1.1 MD17G1278800.v1.1
prunus_persica	Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1 Prupe.3G007100_v2.0.a1
pyrus_communis	pycom09g19350 pycom17g27640
rosa_chinensis	RchiOBHm_Chr3g0487021
rosa_laevigata	RLG00000023038
rosa_multiflora	Rmu_co8314393.1_g000001 Rmu_sc0002834.1_g000013 Rmu_sc0005856.1_g000019 Rmu_sc0012853.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395240
rosa_rugosa	Rorug03G0229700 Rorug03G0229800 Rorug03G0229900 Rorug03G0230000 Rorug03G0230100
rosa_samantha	Rh3AG278800 Rh3BG314700 Rh3CG313000 Rh3DG309600
rosa_wichuraiana	Rw3G024710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1282
Acc36I	ACCTGC	2 cut(s)	1282, 1395
AccB1I	GGYRCC	1 cut(s)	101
AccII	CGCG	2 cut(s)	697, 2016
AccIII	TCCGGA	1 cut(s)	388
AclWI	GGATC	4 cut(s)	1000, 1247, 1308, 1316
AcsI	RAATTY	4 cut(s)	274, 401, 568, 616
AcuI	CTGAAG	3 cut(s)	471, 1155, 1491
AcyI	GRCGYC	2 cut(s)	102, 1574
AfaI	GTAC	4 cut(s)	179, 224, 365, 858
AfeI	AGCGCT	1 cut(s)	2425
AfiI	CCNNNNNNNGG	5 cut(s)	609, 1016, 1399, 1926, 1990
AflIII	ACRYGT	1 cut(s)	2368
AgsI	TTSAA	8 cut(s)	97, 548, 715, 1374, 1940, 1997, 2152, 2441
AjiI	CACGTC	1 cut(s)	758
AjnI	CCWGG	4 cut(s)	795, 1398, 1660, 2072
AjuI	GAANNNNNNNTTGG	2 cut(s)	152, 184
AloI	GAACNNNNNNTCC	2 cut(s)	1089, 1121
Alw21I	GWGCWC	3 cut(s)	240, 818, 1919
Alw26I	GTCTC	1 cut(s)	422
AlwI	GGATC	4 cut(s)	1000, 1247, 1308, 1316
Ama87I	CYCGRG	1 cut(s)	890
Aor13HI	TCCGGA	1 cut(s)	388
Aor51HI	AGCGCT	1 cut(s)	2425
AoxI	GGCC	5 cut(s)	245, 458, 1472, 1880, 2358
ApoI	RAATTY	4 cut(s)	274, 401, 568, 616
ArsI	GACNNNNNNTTYG	4 cut(s)	260, 292, 982, 1014
Asp700I	GAANNNNTTC	2 cut(s)	453, 2415
AspLEI	GCGC	3 cut(s)	104, 978, 2426
AspS9I	GGNCC	4 cut(s)	78, 246, 459, 1880
AsuHPI	GGTGA	4 cut(s)	941, 1306, 1784, 2188
AsuNHI	GCTAGC	2 cut(s)	1841, 2446
AvaI	CYCGRG	1 cut(s)	890
AvaII	GGWCC	1 cut(s)	78
AxyI	CCTNAGG	2 cut(s)	242, 1578
BaeGI	GKGCMC	1 cut(s)	1033
BanI	GGYRCC	1 cut(s)	101
BanII	GRGCYC	4 cut(s)	11, 62, 738, 1919
BauI	CACGAG	2 cut(s)	1500, 2262
BbsI	GAAGAC	3 cut(s)	204, 367, 1937
Bbv12I	GWGCWC	3 cut(s)	240, 818, 1919
BbvCI	CCTCAGC	1 cut(s)	1601
BccI	CCATC	6 cut(s)	308, 870, 920, 1603, 1792, 2100
BcgI	CGANNNNNNTGC	2 cut(s)	1835, 1869
BciT130I	CCWGG	4 cut(s)	797, 1400, 1662, 2074
BclI	TGATCA	3 cut(s)	1210, 1279, 1567
BcoDI	GTCTC	1 cut(s)	422
BfaI	CTAG	5 cut(s)	563, 1038, 1842, 2379, 2447
BfmI	CTRYAG	5 cut(s)	579, 687, 1617, 1854, 2078
BfoI	RGCGCY	2 cut(s)	105, 2427
BfuAI	ACCTGC	2 cut(s)	1282, 1395
BlpI	GCTNAGC	3 cut(s)	732, 1200, 1269
Bme1390I	CCNGG	4 cut(s)	797, 1400, 1662, 2074
Bme18I	GGWCC	1 cut(s)	78
BmeT110I	CYCGRG	1 cut(s)	890
BmgBI	CACGTC	1 cut(s)	758
BmgT120I	GGNCC	4 cut(s)	78, 246, 459, 1880
BmiI	GGNNCC	2 cut(s)	103, 2457
BmrFI	CCNGG	4 cut(s)	797, 1400, 1662, 2074
BmrI	ACTGGG	1 cut(s)	336
BmtI	GCTAGC	2 cut(s)	1845, 2450
BmuI	ACTGGG	1 cut(s)	336
BoxI	GACNNNNGTC	2 cut(s)	329, 696
BpiI	GAAGAC	3 cut(s)	204, 367, 1937
BpmI	CTGGAG	1 cut(s)	1461
Bpu10I	CCTNAGC	1 cut(s)	1601
Bpu1102I	GCTNAGC	3 cut(s)	732, 1200, 1269
BpuEI	CTTGAG	1 cut(s)	200
BsaHI	GRCGYC	2 cut(s)	102, 1574
BsaJI	CCNNGG	1 cut(s)	795
BsaWI	WCCGGW	3 cut(s)	270, 388, 700
BsaXI	ACNNNNNCTCC	4 cut(s)	279, 309, 1883, 1913
Bsc4I	CCNNNNNNNGG	5 cut(s)	609, 1016, 1399, 1926, 1990
Bse118I	RCCGGY	1 cut(s)	1195
Bse1I	ACTGG	6 cut(s)	331, 943, 997, 1188, 1829, 2052
Bse21I	CCTNAGG	2 cut(s)	242, 1578
BseAI	TCCGGA	1 cut(s)	388
BseBI	CCWGG	4 cut(s)	797, 1400, 1662, 2074
BseDI	CCNNGG	1 cut(s)	795
BseGI	GGATG	6 cut(s)	304, 952, 1614, 1755, 1784, 2239
BseLI	CCNNNNNNNGG	5 cut(s)	609, 1016, 1399, 1926, 1990
BseNI	ACTGG	6 cut(s)	331, 943, 997, 1188, 1829, 2052
BseRI	GAGGAG	3 cut(s)	296, 599, 1904
BseSI	GKGCMC	1 cut(s)	1033
BsgI	GTGCAG	1 cut(s)	1671
Bsh1236I	CGCG	2 cut(s)	697, 2016
BshFI	GGCC	5 cut(s)	247, 460, 1474, 1882, 2360
BshNI	GGYRCC	1 cut(s)	101
BsiHKAI	GWGCWC	3 cut(s)	240, 818, 1919
BsiHKCI	CYCGRG	1 cut(s)	890
BsiSI	CCGG	4 cut(s)	271, 389, 701, 1196
BslI	CCNNNNNNNGG	5 cut(s)	609, 1016, 1399, 1926, 1990
BsmAI	GTCTC	1 cut(s)	422
BsmBI	CGTCTC	1 cut(s)	422
BsmI	GAATGC	3 cut(s)	2003, 2257, 2415
BsnI	GGCC	5 cut(s)	247, 460, 1474, 1882, 2360
BsoBI	CYCGRG	1 cut(s)	890
Bsp1286I	GDGCHC	7 cut(s)	11, 62, 240, 738, 818, 1033, 1919
Bsp13I	TCCGGA	1 cut(s)	388
Bsp1720I	GCTNAGC	3 cut(s)	732, 1200, 1269
BspANI	GGCC	5 cut(s)	247, 460, 1474, 1882, 2360
BspEI	TCCGGA	1 cut(s)	388
BspFNI	CGCG	2 cut(s)	697, 2016
BspLI	GGNNCC	2 cut(s)	103, 2457
BspMAI	CTGCAG	3 cut(s)	583, 1858, 2082
BspMI	ACCTGC	2 cut(s)	1282, 1395
BspOI	GCTAGC	2 cut(s)	1845, 2450
BspPI	GGATC	4 cut(s)	1000, 1247, 1308, 1316
BspQI	GCTCTTC	1 cut(s)	21
BspT107I	GGYRCC	1 cut(s)	101
BsrFI	RCCGGY	1 cut(s)	1195
BsrI	ACTGG	6 cut(s)	331, 943, 997, 1188, 1829, 2052
BssAI	RCCGGY	1 cut(s)	1195
BssECI	CCNNGG	1 cut(s)	795
BssNI	GRCGYC	2 cut(s)	102, 1574
BssSI	CACGAG	2 cut(s)	1500, 2262
Bst2BI	CACGAG	2 cut(s)	1500, 2262
Bst2UI	CCWGG	4 cut(s)	797, 1400, 1662, 2074
Bst4CI	ACNGT	1 cut(s)	416
Bst6I	CTCTTC	4 cut(s)	21, 149, 1304, 2280
BstACI	GRCGYC	2 cut(s)	102, 1574
BstAPI	GCANNNNNTGC	4 cut(s)	517, 1631, 1862, 1900
BstC8I	GCNNGC	9 cut(s)	7, 264, 522, 887, 1472, 1843, 1880, 2171, 2448
BstF5I	GGATG	6 cut(s)	304, 952, 1614, 1755, 1784, 2239
BstFNI	CGCG	2 cut(s)	697, 2016
BstH2I	RGCGCY	2 cut(s)	105, 2427
BstHHI	GCGC	3 cut(s)	104, 978, 2426
BstMAI	GTCTC	1 cut(s)	422
BstNI	CCWGG	4 cut(s)	797, 1400, 1662, 2074
BstNSI	RCATGY	3 cut(s)	1598, 2336, 2372
BstPAI	GACNNNNGTC	2 cut(s)	329, 696
BstSCI	CCNGG	4 cut(s)	795, 1398, 1660, 2072
BstSFI	CTRYAG	5 cut(s)	579, 687, 1617, 1854, 2078
BstSLI	GKGCMC	1 cut(s)	1033
BstUI	CGCG	2 cut(s)	697, 2016
BstV2I	GAAGAC	3 cut(s)	204, 367, 1937
Bsu36I	CCTNAGG	2 cut(s)	242, 1578
BsuRI	GGCC	5 cut(s)	247, 460, 1474, 1882, 2360
BtrI	CACGTC	1 cut(s)	758
BtsCI	GGATG	6 cut(s)	304, 952, 1614, 1755, 1784, 2239
BtsI	GCAGTG	1 cut(s)	1372
BtsIMutI	CAGTG	4 cut(s)	950, 1181, 1372, 1876
BveI	ACCTGC	2 cut(s)	1282, 1395
Cac8I	GCNNGC	9 cut(s)	7, 264, 522, 887, 1472, 1843, 1880, 2171, 2448
CfoI	GCGC	3 cut(s)	104, 978, 2426
Cfr10I	RCCGGY	1 cut(s)	1195
Cfr13I	GGNCC	4 cut(s)	78, 246, 459, 1880
CseI	GACGC	6 cut(s)	358, 637, 686, 846, 1062, 1582
Csp6I	GTAC	4 cut(s)	178, 223, 364, 857
CviQI	GTAC	4 cut(s)	178, 223, 364, 857
DinI	GGCGCC	1 cut(s)	103
Eam1104I	CTCTTC	4 cut(s)	21, 149, 1304, 2280
EarI	CTCTTC	4 cut(s)	21, 149, 1304, 2280
EciI	GGCGGA	1 cut(s)	133
Ecl136II	GAGCTC	1 cut(s)	1917
Eco147I	AGGCCT	1 cut(s)	1474
Eco24I	GRGCYC	4 cut(s)	11, 62, 738, 1919
Eco47I	GGWCC	1 cut(s)	78
Eco47III	AGCGCT	1 cut(s)	2425
Eco53kI	GAGCTC	1 cut(s)	1917
Eco57I	CTGAAG	3 cut(s)	471, 1155, 1491
Eco81I	CCTNAGG	2 cut(s)	242, 1578
Eco88I	CYCGRG	1 cut(s)	890
EcoICRI	GAGCTC	1 cut(s)	1917
EcoRII	CCWGG	4 cut(s)	795, 1398, 1660, 2072
EcoT22I	ATGCAT	1 cut(s)	2338
EcoT38I	GRGCYC	4 cut(s)	11, 62, 738, 1919
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
Esp3I	CGTCTC	1 cut(s)	422
FalI	AAGNNNNNCTT	2 cut(s)	1072, 1104
FauI	CCCGC	2 cut(s)	1871, 2166
FauNDI	CATATG	1 cut(s)	1885
FbaI	TGATCA	3 cut(s)	1210, 1279, 1567
FokI	GGATG	6 cut(s)	311, 939, 1621, 1762, 1771, 2246
FriOI	GRGCYC	4 cut(s)	11, 62, 738, 1919
FspBI	CTAG	5 cut(s)	563, 1038, 1842, 2379, 2447
GlaI	GCGC	3 cut(s)	103, 977, 2425
GsuI	CTGGAG	1 cut(s)	1461
HaeII	RGCGCY	2 cut(s)	105, 2427
HaeIII	GGCC	5 cut(s)	247, 460, 1474, 1882, 2360
HapII	CCGG	4 cut(s)	271, 389, 701, 1196
HgaI	GACGC	6 cut(s)	358, 637, 686, 846, 1062, 1582
HhaI	GCGC	3 cut(s)	104, 978, 2426
Hin1I	GRCGYC	2 cut(s)	102, 1574
Hin6I	GCGC	3 cut(s)	102, 976, 2424
HinP1I	GCGC	3 cut(s)	102, 976, 2424
HincII	GTYRAC	4 cut(s)	669, 1158, 1804, 2131
HindII	GTYRAC	4 cut(s)	669, 1158, 1804, 2131
HpaI	GTTAAC	1 cut(s)	669
HpaII	CCGG	4 cut(s)	271, 389, 701, 1196
HphI	GGTGA	4 cut(s)	941, 1306, 1784, 2188
Hpy166II	GTNNAC	5 cut(s)	324, 669, 1158, 1804, 2131
Hpy8I	GTNNAC	5 cut(s)	324, 669, 1158, 1804, 2131
Hpy99I	CGWCG	4 cut(s)	86, 230, 374, 2090
HpyAV	CCTTC	7 cut(s)	83, 334, 966, 1109, 1291, 1659, 2405
HpyCH4III	ACNGT	1 cut(s)	416
HpyCH4IV	ACGT	2 cut(s)	225, 757
HpySE526I	ACGT	2 cut(s)	225, 757
Hsp92I	GRCGYC	2 cut(s)	102, 1574
HspAI	GCGC	3 cut(s)	102, 976, 2424
KasI	GGCGCC	1 cut(s)	101
Kpn2I	TCCGGA	1 cut(s)	388
Ksp22I	TGATCA	3 cut(s)	1210, 1279, 1567
KspAI	GTTAAC	1 cut(s)	669
LguI	GCTCTTC	1 cut(s)	21
LmnI	GCTCC	8 cut(s)	45, 235, 625, 1395, 1891, 1922, 2254, 2461
MaeI	CTAG	5 cut(s)	563, 1038, 1842, 2379, 2447
MaeII	ACGT	2 cut(s)	225, 757
MaeIII	GTNAC	6 cut(s)	124, 190, 1177, 1402, 1497, 2155
MfeI	CAATTG	4 cut(s)	44, 761, 1935, 2028
MhlI	GDGCHC	7 cut(s)	11, 62, 240, 738, 818, 1033, 1919
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	3 cut(s)	686, 2131, 2234
MmeI	TCCRAC	1 cut(s)	2008
Mph1103I	ATGCAT	1 cut(s)	2338
MroI	TCCGGA	1 cut(s)	388
MroXI	GAANNNNTTC	2 cut(s)	453, 2415
MslI	CAYNNNNRTG	3 cut(s)	1346, 2097, 2262
MspA1I	CMGCKG	4 cut(s)	731, 1355, 1622, 1878
MspI	CCGG	4 cut(s)	271, 389, 701, 1196
MspR9I	CCNGG	4 cut(s)	797, 1400, 1662, 2074
MunI	CAATTG	4 cut(s)	44, 761, 1935, 2028
Mva1269I	GAATGC	3 cut(s)	2003, 2257, 2415
MvaI	CCWGG	4 cut(s)	797, 1400, 1662, 2074
MvnI	CGCG	2 cut(s)	697, 2016
NarI	GGCGCC	1 cut(s)	102
NdeI	CATATG	1 cut(s)	1885
NheI	GCTAGC	2 cut(s)	1841, 2446
NlaIV	GGNNCC	2 cut(s)	103, 2457
NmuCI	GTSAC	3 cut(s)	124, 190, 1497
NsiI	ATGCAT	1 cut(s)	2338
NspI	RCATGY	3 cut(s)	1598, 2336, 2372
PaqCI	CACCTGC	1 cut(s)	1282
PceI	AGGCCT	1 cut(s)	1474
PciI	ACATGT	1 cut(s)	2368
PciSI	GCTCTTC	1 cut(s)	21
PctI	GAATGC	3 cut(s)	2003, 2257, 2415
PdmI	GAANNNNTTC	2 cut(s)	453, 2415
PfoI	TCCNGGA	1 cut(s)	1660
PleI	GAGTC	3 cut(s)	686, 2130, 2233
PluTI	GGCGCC	1 cut(s)	105
PpsI	GAGTC	3 cut(s)	686, 2130, 2233
PscI	ACATGT	1 cut(s)	2368
PshAI	GACNNNNGTC	2 cut(s)	329, 696
Psp124BI	GAGCTC	1 cut(s)	1919
Psp6I	CCWGG	4 cut(s)	795, 1398, 1660, 2072
PspGI	CCWGG	4 cut(s)	795, 1398, 1660, 2072
PspN4I	GGNNCC	2 cut(s)	103, 2457
PspPI	GGNCC	4 cut(s)	78, 246, 459, 1880
PstI	CTGCAG	3 cut(s)	583, 1858, 2082
PvuII	CAGCTG	3 cut(s)	731, 1355, 1622
RsaI	GTAC	4 cut(s)	179, 224, 365, 858
RsaNI	GTAC	4 cut(s)	178, 223, 364, 857
RseI	CAYNNNNRTG	3 cut(s)	1346, 2097, 2262
SacI	GAGCTC	1 cut(s)	1919
SapI	GCTCTTC	1 cut(s)	21
Sau96I	GGNCC	4 cut(s)	78, 246, 459, 1880
SchI	GAGTC	3 cut(s)	686, 2131, 2234
ScrFI	CCNGG	4 cut(s)	797, 1400, 1662, 2074
SduI	GDGCHC	7 cut(s)	11, 62, 240, 738, 818, 1033, 1919
SfcI	CTRYAG	5 cut(s)	579, 687, 1617, 1854, 2078
SfoI	GGCGCC	1 cut(s)	103
SinI	GGWCC	1 cut(s)	78
SmiMI	CAYNNNNRTG	3 cut(s)	1346, 2097, 2262
SmlI	CTYRAG	1 cut(s)	215
SmoI	CTYRAG	1 cut(s)	215
SseBI	AGGCCT	1 cut(s)	1474
SspDI	GGCGCC	1 cut(s)	101
SspI	AATATT	1 cut(s)	1123
SspMI	CTAG	5 cut(s)	563, 1038, 1842, 2379, 2447
SstI	GAGCTC	1 cut(s)	1919
StuI	AGGCCT	1 cut(s)	1474
StyD4I	CCNGG	4 cut(s)	795, 1398, 1660, 2072
TaaI	ACNGT	1 cut(s)	416
TaiI	ACGT	2 cut(s)	228, 760
TaqII	GACCGA	1 cut(s)	95
TatI	WGTACW	2 cut(s)	177, 363
TauI	GCSGC	2 cut(s)	256, 259
TscAI	CASTG	4 cut(s)	950, 1188, 1372, 1876
TseFI	GTSAC	3 cut(s)	124, 190, 1497
Tsp45I	GTSAC	3 cut(s)	124, 190, 1497
TspDTI	ATGAA	5 cut(s)	941, 1128, 1347, 2420, 2424
TspGWI	ACGGA	5 cut(s)	209, 859, 1382, 1527, 2075
TspRI	CASTG	4 cut(s)	950, 1188, 1372, 1876
VpaK11BI	GGWCC	1 cut(s)	78
XapI	RAATTY	4 cut(s)	274, 401, 568, 616
XceI	RCATGY	3 cut(s)	1598, 2336, 2372
XmnI	GAANNNNTTC	2 cut(s)	453, 2415
XspI	CTAG	5 cut(s)	563, 1038, 1842, 2379, 2447
Zsp2I	ATGCAT	1 cut(s)	2338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.