MD09G1290300.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005145
Physical Location & Seq
Forward (+)
37186177 .. 37191886
5710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1290300.v1.1.491

Sequence Viewer

Length: 2622 bp
ATGTCAAATCCACCGCAGCCTCCCATTGGATACTCTGTCAGTATCACTCCCTCACGCCCAGACACACTATCTCTTGACCCTGAGAAAATTTCTATTCCTCCACCACCTTTAATTGCTCCGAGATTCCCTCCACCAAAGTTTCAACAAGATCAAACTCCTTCTCCATCCATCAAAACCCCAAATGCACCATCACCAGCTAATGGGTTGAAAACTGGCAGCCCTATTCCTCATCTGAGTACACCCCCTGGACCTCCTGTTTTTACTTCCCCTGTGCGGCCTGCTGCTGTGCCTTTTCGTGCTTCACCTGCAACTCCTCAGCCAGTTGCTTTCACCCCGGGCACATTTTTGCCAACATCTTCGCCTCCCAATTTTTCGAATGGCTCGCATGAACTACATCACCAACTTTCTAATGATACAGAGGATGATGCTGTGCCTGTTGGAGAATCACCATATGTCCTATTCTCAGCTCATAAGGCGTTGAAACAAAAGAAACAAGCAAATGTACCCAGTTTGGGTTTTGGGGCCTTGGTTTCACCTGGAAGGGGAATTTCACCTGGTCCCCAGATAATACAACGTGATCCTCATCGCTGCCACAGCTGTGGAGCTTATGCAAATATTTTTTGCAACATCTTATTGTGCTCAGGTCAGTGGCAATGTGTAATTTGCCGAGAACTGAATGGAAGTGAGGGAGAATACATAGTTCCTTGCAAGGAAGATCTTTGTAATTTTCCAGAGTTGTCATCTCCAATGGTTGATTATGTTCAAACTGGGAACAATAGACCTGGTTTTGTTCCTGTTTCTGACTCAAGAATGTCTGCACCTATAGTTCTTGTCATAGATGAGTGTTTAGACGAACCACATCTGTGGGATTTACAAAGCTCCTTGCATGCATTTGTTGATTCACTTCCCCCGACAACAAGAATTGGAATAATACTGTATGGTCGCACAGTATCAGTCTATGACTTTTCAGAGGAATCATTTGCATCTGCTGATGTGCTTCCAGGAGCTAAATCACCAAGTCAGGATGCCTTAAAAGCATTGATATATGGAACTGGTATATACTTGTCTCCAATGCATGCTTCCTTACCAGTGGCACATGCCATATTCTCATCATTGAGGCCATACAAAATGAAAACTCCAGAAACTTCTAGAGATCGGTGCCTGGGGACGGCAGTTGAGGTTGCTCTTGCTATAGTTCAAGGGCCATCAGGAGAAATGTCTCGAGGGGTAATTAAAAGGTCTGGAGGTAATAGCAGAATCATTGTTTGTGCTGGTGGGCCTAATACATATGGTCCTGGATCTGTTCCTCATTCTTTCAGTCACCCAAATTATCCTCATATGGAAAAGACTGCATTGAAATGGATGGAGCATCTGGGTCAAGAGGCACACCGACAAAATACAGTGGTTGACATTTTGTGCGCTGGGCAATGCCCTGTAAGAGTTCCTGTTTTGCAGCCTCTTGCAAAAGCTTCTGGAGGCATTTTTGTTCTCCATGACGACTTTGGGGAAGCCTTTGGTGTGAACTTACAAAGGGCATCTACCAGGGCTGCAGGTTCCCGTGGGTTCTTGGCAATACGCTGTTCCGATGACATTCTCATAACTCAAGTTGTGGGTCCTGGTGAAGAGGCACATATTGACACTCATGAAACCTTCAAAAATGACACTTCTCTTTACATACAAATGCTTAGTGTTGAAGAGACACAGAGCTTCTCACTCTCCTTGGAAAATAAGAGGGACATTAGGACTGAGTATGTGTATTTCCAGTTTACAATCCAGTATTTAAATGTGTATCAAGCTGATATATCAAGAGTAATTACTGTTAGATTGCCAACAGTTGATAGTGTTTCAGCATATCTTGCAAGTGTTCAAGATGAAGTTGCAGCAGTTCTTATTGCAAAAAGGACTCTCTTGCGAGCTAAAAACTTTTCAGATGCAATTGATATGCGAGGAACAATAGATGAAAGAATTAAAGACATTGCTCTGAAATTTGGGTCTCAAGTACCAAAGTCAAAGCTTTATCGGTTTCCAAAGGAGATCCCTTTATTGCCAGAGCTCCTGCTTCATCTAAGAAGGGGCCCACTGCTGGGAAGCATTGTTGGCCATGAAGATGAGAGATCTGTAATACGAAACTTGTTTCTGAATGCATCCTTTGATCTCTCACTCCGAATGGTAGCACCTCGTTGTTTAATGCATCGGGAAGGGGGAACATTTGAGGAACTACCAGCTTATGACCTTGCTATGCAGTCAGATGCAGCAGTTGTCCTTGATCATGGCACAGATGTCTTCATTTGGTTGGGTGCTGAACTTGCTGCTGATGAAGGAAAAAGTGCAGCTGCTTTAGCGGCTTGCAGAACATTAGCTGAAGAGCTCACTGAACTGAGGTTTCCGGCTCCTCGGATCCTGTCGTTCAAGGAAGGAAGTTCTCAGGCTCGATATTTCGTATCTCGGCTCATACCCGCACACAAGGATCCTCCTTATGAGCAGGAGGCAAGATTCCCACAGCTACGAACATTGAGAACAGAAGAGCGGACGAAGCTGAAAAACAGTTTTATTAGTTTTGATGAGCCTAGTTTTTGCGAGTGGGTACGAAGCTTGAAAGTGGTGCCTCCGGAACCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

874

Amino Acids

95.48

Weight (kDa)

6.26

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 194 - 232 2.2e-08 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 276 - 502 7.7e-13 Sec23/Sec24 trunk domain
Sec23_BS PF08033 521 - 611 7.3e-12 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 625 - 721 1.2e-12 Sec23/Sec24 helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 313
Acc36I ACCTGC 2 cut(s) 313, 1541
AccB1I GGYRCC 2 cut(s) 1158, 2602
AccB7I CCANNNNNTGG 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 2527
AccIII TCCGGA 1 cut(s) 2608
AciI CCGC 5 cut(s) 14, 274, 2342, 2457, 2527
AclWI GGATC 7 cut(s) 572, 1306, 2029, 2392, 2405, 2462, 2475
AcoI YGGCCR 1 cut(s) 2098
AcsI RAATTY 3 cut(s) 87, 546, 1985
AcuI CTGAAG 1 cut(s) 2382
AfaI GTAC 4 cut(s) 238, 504, 2001, 2586
AfiI CCNNNNNNNGG 8 cut(s) 26, 200, 273, 512, 542, 1168, 2083, 2084
AjnI CCWGG 9 cut(s) 244, 535, 553, 781, 1000, 1161, 1294, 1541, 1615
AjuI GAANNNNNNNTTGG 2 cut(s) 1063, 1095
AleI CACNNNNGTG 2 cut(s) 597, 862
Alw21I GWGCWC 3 cut(s) 641, 2055, 2370
Alw26I GTCTC 4 cut(s) 1071, 1224, 1691, 1998
AlwI GGATC 7 cut(s) 572, 1306, 2029, 2392, 2405, 2462, 2475
Ama87I CYCGRG 2 cut(s) 334, 1221
Aor13HI TCCGGA 1 cut(s) 2608
AoxI GGCC 7 cut(s) 275, 522, 1118, 1202, 1277, 2074, 2098
ApaI GGGCCC 1 cut(s) 2078
ApoI RAATTY 3 cut(s) 87, 546, 1985
AspLEI GCGC 1 cut(s) 1421
AspS9I GGNCC 9 cut(s) 248, 522, 557, 1202, 1277, 1292, 1613, 2074, 2075
AsuC2I CCSGG 2 cut(s) 335, 336
AsuII TTCGAA 1 cut(s) 374
AvaI CYCGRG 2 cut(s) 334, 1221
AvaII GGWCC 4 cut(s) 248, 557, 1292, 1613
BaeGI GKGCMC 2 cut(s) 341, 2078
BalI TGGCCA 1 cut(s) 2100
BamHI GGATCC 2 cut(s) 2397, 2467
BanI GGYRCC 2 cut(s) 1158, 2602
BanII GRGCYC 3 cut(s) 2055, 2078, 2370
BbsI GAAGAC 1 cut(s) 2275
Bbv12I GWGCWC 3 cut(s) 641, 2055, 2370
BbvCI CCTCAGC 1 cut(s) 315
BccI CCATC 5 cut(s) 172, 176, 196, 1213, 1357
BceAI ACGGC 1 cut(s) 1185
BciT130I CCWGG 9 cut(s) 246, 537, 555, 783, 1002, 1163, 1296, 1543, 1617
BciVI GTATCC 1 cut(s) 23
BclI TGATCA 1 cut(s) 2266
BcnI CCSGG 2 cut(s) 335, 336
BcoDI GTCTC 4 cut(s) 1071, 1224, 1691, 1998
BfaI CTAG 3 cut(s) 1149, 2568, 2620
BfmI CTRYAG 3 cut(s) 822, 1191, 1548
BfuAI ACCTGC 2 cut(s) 313, 1541
BfuI GTATCC 1 cut(s) 23
BglII AGATCT 2 cut(s) 715, 2114
Bme18I GGWCC 4 cut(s) 248, 557, 1292, 1613
BmeT110I CYCGRG 2 cut(s) 334, 1221
BmgT120I GGNCC 9 cut(s) 248, 522, 557, 1202, 1277, 1292, 1613, 2074, 2075
BmrI ACTGGG 2 cut(s) 501, 777
BmsI GCATC 9 cut(s) 415, 992, 1015, 1378, 1544, 1921, 2153, 2200, 2239
BmuI ACTGGG 2 cut(s) 501, 777
BpiI GAAGAC 1 cut(s) 2275
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpmI CTGGAG 3 cut(s) 1122, 1263, 1494
Bpu10I CCTNAGC 2 cut(s) 315, 640
Bpu14I TTCGAA 1 cut(s) 374
BpuEI CTTGAG 3 cut(s) 790, 1587, 1980
BpuMI CCSGG 2 cut(s) 335, 336
BsaBI GATNNNNATC 1 cut(s) 582
BsaI GGTCTC 1 cut(s) 1998
BsaJI CCNNGG 9 cut(s) 244, 333, 334, 525, 1162, 1542, 1558, 1719, 2393
BsaWI WCCGGW 1 cut(s) 2608
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 8 cut(s) 26, 200, 273, 512, 542, 1168, 2083, 2084
Bse1I ACTGG 8 cut(s) 217, 320, 507, 772, 1057, 1088, 1762, 1774
Bse3DI GCAATG 3 cut(s) 659, 1433, 1974
Bse8I GATNNNNATC 1 cut(s) 582
BseAI TCCGGA 1 cut(s) 2608
BseBI CCWGG 9 cut(s) 246, 537, 555, 783, 1002, 1163, 1296, 1543, 1617
BseDI CCNNGG 9 cut(s) 244, 333, 334, 525, 1162, 1542, 1558, 1719, 2393
BseGI GGATG 5 cut(s) 164, 427, 1030, 1368, 2144
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 8 cut(s) 26, 200, 273, 512, 542, 1168, 2083, 2084
BseMI GCAATG 3 cut(s) 659, 1433, 1974
BseMII CTCAG 8 cut(s) 72, 224, 329, 477, 654, 1737, 2369, 2438
BseNI ACTGG 8 cut(s) 217, 320, 507, 772, 1057, 1088, 1762, 1774
BseRI GAGGAG 2 cut(s) 303, 2382
BseSI GKGCMC 2 cut(s) 341, 2078
BseYI CCCAGC 2 cut(s) 1421, 2083
BsgI GTGCAG 2 cut(s) 801, 2349
BshFI GGCC 7 cut(s) 277, 524, 1120, 1204, 1279, 2076, 2100
BshNI GGYRCC 2 cut(s) 1158, 2602
BsiHKAI GWGCWC 3 cut(s) 641, 2055, 2370
BsiHKCI CYCGRG 2 cut(s) 334, 1221
BsiSI CCGG 3 cut(s) 335, 2387, 2609
BslFI GGGAC 3 cut(s) 543, 1180, 1748
BslI CCNNNNNNNGG 8 cut(s) 26, 200, 273, 512, 542, 1168, 2083, 2084
BsmAI GTCTC 4 cut(s) 1071, 1224, 1691, 1998
BsmFI GGGAC 3 cut(s) 543, 1180, 1748
BsmI GAATGC 1 cut(s) 2146
BsnI GGCC 7 cut(s) 277, 524, 1120, 1204, 1279, 2076, 2100
Bso31I GGTCTC 1 cut(s) 1998
BsoBI CYCGRG 2 cut(s) 334, 1221
Bsp119I TTCGAA 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 2074
Bsp1286I GDGCHC 5 cut(s) 341, 641, 2055, 2078, 2370
Bsp13I TCCGGA 1 cut(s) 2608
BspACI CCGC 5 cut(s) 14, 274, 2342, 2457, 2527
BspANI GGCC 7 cut(s) 277, 524, 1120, 1204, 1279, 2076, 2100
BspCNI CTCAG 8 cut(s) 73, 225, 328, 476, 653, 1738, 2370, 2437
BspEI TCCGGA 1 cut(s) 2608
BspHI TCATGA 1 cut(s) 1642
BspMAI CTGCAG 1 cut(s) 1552
BspMI ACCTGC 2 cut(s) 313, 1541
BspPI GGATC 7 cut(s) 572, 1306, 2029, 2392, 2405, 2462, 2475
BspQI GCTCTTC 2 cut(s) 2358, 2517
BspT104I TTCGAA 1 cut(s) 374
BspT107I GGYRCC 2 cut(s) 1158, 2602
BspTNI GGTCTC 1 cut(s) 1998
BsrBI CCGCTC 1 cut(s) 2527
BsrDI GCAATG 3 cut(s) 659, 1433, 1974
BsrI ACTGG 8 cut(s) 217, 320, 507, 772, 1057, 1088, 1762, 1774
BssECI CCNNGG 9 cut(s) 244, 333, 334, 525, 1162, 1542, 1558, 1719, 2393
BssT1I CCWWGG 2 cut(s) 525, 1719
Bst2UI CCWGG 9 cut(s) 246, 537, 555, 783, 1002, 1163, 1296, 1543, 1617
Bst4CI ACNGT 6 cut(s) 936, 949, 1402, 1819, 1834, 2546
Bst6I CTCTTC 4 cut(s) 1617, 1689, 2358, 2517
BstAPI GCANNNNNTGC 1 cut(s) 1856
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 6 cut(s) 279, 383, 888, 1077, 1914, 2347
BstDSI CCRYGG 1 cut(s) 1558
BstF5I GGATG 5 cut(s) 164, 427, 1030, 1368, 2144
BstHHI GCGC 1 cut(s) 1421
BstMAI GTCTC 4 cut(s) 1071, 1224, 1691, 1998
BstNI CCWGG 9 cut(s) 246, 537, 555, 783, 1002, 1163, 1296, 1543, 1617
BstNSI RCATGY 3 cut(s) 890, 1079, 1100
BstSFI CTRYAG 3 cut(s) 822, 1191, 1548
BstSLI GKGCMC 2 cut(s) 341, 2078
BstV2I GAAGAC 1 cut(s) 2275
BstX2I RGATCY 6 cut(s) 715, 1298, 2034, 2114, 2397, 2467
BstXI CCANNNNNNTGG 2 cut(s) 599, 864
BstYI RGATCY 6 cut(s) 715, 1298, 2034, 2114, 2397, 2467
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 7 cut(s) 277, 524, 1120, 1204, 1279, 2076, 2100
BtgI CCRYGG 1 cut(s) 1558
BtgZI GCGATG 1 cut(s) 569
BtsCI GGATG 5 cut(s) 164, 427, 1030, 1368, 2144
BtsI GCAGTG 1 cut(s) 2078
BtsIMutI CAGTG 5 cut(s) 653, 1095, 1407, 2078, 2370
BveI ACCTGC 2 cut(s) 313, 1541
Cac8I GCNNGC 6 cut(s) 279, 383, 888, 1077, 1914, 2347
CciI TCATGA 1 cut(s) 1642
CfoI GCGC 1 cut(s) 1421
Cfr13I GGNCC 9 cut(s) 248, 522, 557, 1202, 1277, 1292, 1613, 2074, 2075
Cfr9I CCCGGG 1 cut(s) 334
CsiI ACCWGGT 2 cut(s) 553, 781
Csp6I GTAC 4 cut(s) 237, 503, 2000, 2585
CviAII CATG 8 cut(s) 386, 887, 1076, 1097, 1493, 1643, 2102, 2270
CviQI GTAC 4 cut(s) 237, 503, 2000, 2585
DraI TTTAAA 1 cut(s) 1782
EaeI YGGCCR 1 cut(s) 2098
Eam1104I CTCTTC 4 cut(s) 1617, 1689, 2358, 2517
EarI CTCTTC 4 cut(s) 1617, 1689, 2358, 2517
Ecl136II GAGCTC 2 cut(s) 2053, 2368
Eco130I CCWWGG 2 cut(s) 525, 1719
Eco24I GRGCYC 3 cut(s) 2055, 2078, 2370
Eco31I GGTCTC 1 cut(s) 1998
Eco47I GGWCC 4 cut(s) 248, 557, 1292, 1613
Eco53kI GAGCTC 2 cut(s) 2053, 2368
Eco57I CTGAAG 1 cut(s) 2382
Eco88I CYCGRG 2 cut(s) 334, 1221
EcoICRI GAGCTC 2 cut(s) 2053, 2368
EcoO109I RGGNCCY 3 cut(s) 522, 1613, 2074
EcoRII CCWGG 9 cut(s) 244, 535, 553, 781, 1000, 1161, 1294, 1541, 1615
EcoT14I CCWWGG 2 cut(s) 525, 1719
EcoT22I ATGCAT 4 cut(s) 892, 1077, 2146, 2193
EcoT38I GRGCYC 3 cut(s) 2055, 2078, 2370
ErhI CCWWGG 2 cut(s) 525, 1719
FaeI CATG 8 cut(s) 389, 890, 1079, 1100, 1496, 1646, 2105, 2273
FaqI GGGAC 3 cut(s) 543, 1180, 1748
FatI CATG 8 cut(s) 385, 886, 1075, 1096, 1492, 1642, 2101, 2269
FauI CCCGC 1 cut(s) 2464
FauNDI CATATG 3 cut(s) 451, 1288, 1338
FbaI TGATCA 1 cut(s) 2266
FokI GGATG 5 cut(s) 151, 434, 1037, 1375, 2131
FriOI GRGCYC 3 cut(s) 2055, 2078, 2370
FspBI CTAG 3 cut(s) 1149, 2568, 2620
GlaI GCGC 1 cut(s) 1420
GsaI CCCAGC 2 cut(s) 1425, 2087
GsuI CTGGAG 3 cut(s) 1122, 1263, 1494
HaeIII GGCC 7 cut(s) 277, 524, 1120, 1204, 1279, 2076, 2100
HapII CCGG 3 cut(s) 335, 2387, 2609
HhaI GCGC 1 cut(s) 1421
Hin1II CATG 8 cut(s) 389, 890, 1079, 1100, 1496, 1646, 2105, 2273
Hin6I GCGC 1 cut(s) 1419
HinP1I GCGC 1 cut(s) 1419
HincII GTYRAC 1 cut(s) 1408
HindII GTYRAC 1 cut(s) 1408
HindIII AAGCTT 3 cut(s) 1467, 2012, 2590
HinfI GANTC 8 cut(s) 123, 443, 803, 899, 974, 1257, 1903, 2493
HpaII CCGG 3 cut(s) 335, 2387, 2609
Hpy166II GTNNAC 4 cut(s) 239, 1408, 1522, 1767
Hpy8I GTNNAC 4 cut(s) 239, 1408, 1522, 1767
HpyAV CCTTC 7 cut(s) 168, 534, 1660, 2064, 2192, 2312, 2408
HpyCH4III ACNGT 6 cut(s) 936, 949, 1402, 1819, 1834, 2546
HpyCH4IV ACGT 1 cut(s) 574
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 8 cut(s) 389, 890, 1079, 1100, 1496, 1646, 2105, 2273
HspAI GCGC 1 cut(s) 1419
Kpn2I TCCGGA 1 cut(s) 2608
Ksp22I TGATCA 1 cut(s) 2266
LguI GCTCTTC 2 cut(s) 2358, 2517
LmnI GCTCC 7 cut(s) 121, 602, 884, 1004, 1366, 2058, 2395
LweI GCATC 9 cut(s) 415, 992, 1015, 1378, 1544, 1921, 2153, 2200, 2239
MabI ACCWGGT 2 cut(s) 553, 781
MaeI CTAG 3 cut(s) 1149, 2568, 2620
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 1 cut(s) 1319
MbiI CCGCTC 1 cut(s) 2527
MboII GAAGA 8 cut(s) 348, 725, 1634, 1706, 2117, 2275, 2375, 2534
MfeI CAATTG 1 cut(s) 1935
MflI RGATCY 6 cut(s) 715, 1298, 2034, 2114, 2397, 2467
MhlI GDGCHC 5 cut(s) 341, 641, 2055, 2078, 2370
MlsI TGGCCA 1 cut(s) 2100
MluNI TGGCCA 1 cut(s) 2100
MlyI GAGTC 2 cut(s) 797, 1897
MmeI TCCRAC 1 cut(s) 418
Mox20I TGGCCA 1 cut(s) 2100
Mph1103I ATGCAT 4 cut(s) 892, 1077, 2146, 2193
MroI TCCGGA 1 cut(s) 2608
MscI TGGCCA 1 cut(s) 2100
MseI TTAA 6 cut(s) 110, 1031, 1233, 1781, 1968, 2186
MslI CAYNNNNRTG 5 cut(s) 597, 862, 1357, 1679, 2106
Msp20I TGGCCA 1 cut(s) 2100
MspA1I CMGCKG 2 cut(s) 597, 2333
MspI CCGG 3 cut(s) 335, 2387, 2609
MunI CAATTG 1 cut(s) 1935
Mva1269I GAATGC 1 cut(s) 2146
MvaI CCWGG 9 cut(s) 246, 537, 555, 783, 1002, 1163, 1296, 1543, 1617
NciI CCSGG 2 cut(s) 335, 336
NdeI CATATG 3 cut(s) 451, 1288, 1338
NlaIII CATG 8 cut(s) 389, 890, 1079, 1100, 1496, 1646, 2105, 2273
NmeAIII GCCGAG 2 cut(s) 692, 2425
NmuCI GTSAC 1 cut(s) 1319
NsiI ATGCAT 4 cut(s) 892, 1077, 2146, 2193
NspI RCATGY 3 cut(s) 890, 1079, 1100
NspV TTCGAA 1 cut(s) 374
OliI CACNNNNGTG 2 cut(s) 597, 862
PaeI GCATGC 2 cut(s) 890, 1079
PaeR7I CTCGAG 1 cut(s) 1221
PagI TCATGA 1 cut(s) 1642
PaqCI CACCTGC 1 cut(s) 313
PciSI GCTCTTC 2 cut(s) 2358, 2517
PctI GAATGC 1 cut(s) 2146
PfeI GAWTC 6 cut(s) 123, 443, 899, 974, 1257, 2493
PflMI CCANNNNNTGG 1 cut(s) 200
PfoI TCCNGGA 2 cut(s) 1000, 1294
PleI GAGTC 2 cut(s) 797, 1897
PpsI GAGTC 2 cut(s) 797, 1897
PpuMI RGGWCCY 1 cut(s) 1613
Psp124BI GAGCTC 2 cut(s) 2055, 2370
Psp5II RGGWCCY 1 cut(s) 1613
Psp6I CCWGG 9 cut(s) 244, 535, 553, 781, 1000, 1161, 1294, 1541, 1615
PspFI CCCAGC 2 cut(s) 1421, 2083
PspGI CCWGG 9 cut(s) 244, 535, 553, 781, 1000, 1161, 1294, 1541, 1615
PspOMI GGGCCC 1 cut(s) 2074
PspPI GGNCC 9 cut(s) 248, 522, 557, 1202, 1277, 1292, 1613, 2074, 2075
PspPPI RGGWCCY 1 cut(s) 1613
PstI CTGCAG 1 cut(s) 1552
PsuI RGATCY 6 cut(s) 715, 1298, 2034, 2114, 2397, 2467
PvuII CAGCTG 2 cut(s) 597, 2333
RsaI GTAC 4 cut(s) 238, 504, 2001, 2586
RsaNI GTAC 4 cut(s) 237, 503, 2000, 2585
RseI CAYNNNNRTG 5 cut(s) 597, 862, 1357, 1679, 2106
SacI GAGCTC 2 cut(s) 2055, 2370
SapI GCTCTTC 2 cut(s) 2358, 2517
SaqAI TTAA 6 cut(s) 110, 1031, 1233, 1781, 1968, 2186
Sau96I GGNCC 9 cut(s) 248, 522, 557, 1202, 1277, 1292, 1613, 2074, 2075
SchI GAGTC 2 cut(s) 797, 1897
SduI GDGCHC 5 cut(s) 341, 641, 2055, 2078, 2370
SexAI ACCWGGT 2 cut(s) 553, 781
SfaNI GCATC 9 cut(s) 415, 992, 1015, 1378, 1544, 1921, 2153, 2200, 2239
SfcI CTRYAG 3 cut(s) 822, 1191, 1548
Sfr274I CTCGAG 1 cut(s) 1221
SfuI TTCGAA 1 cut(s) 374
SinI GGWCC 4 cut(s) 248, 557, 1292, 1613
SlaI CTCGAG 1 cut(s) 1221
SmaI CCCGGG 1 cut(s) 336
SmiI ATTTAAAT 1 cut(s) 1782
SmiMI CAYNNNNRTG 5 cut(s) 597, 862, 1357, 1679, 2106
SmlI CTYRAG 4 cut(s) 805, 1221, 1602, 1995
SmoI CTYRAG 4 cut(s) 805, 1221, 1602, 1995
SphI GCATGC 2 cut(s) 890, 1079
SsiI CCGC 5 cut(s) 14, 274, 2342, 2457, 2527
SspI AATATT 1 cut(s) 616
SspMI CTAG 3 cut(s) 1149, 2568, 2620
SstI GAGCTC 2 cut(s) 2055, 2370
StyI CCWWGG 2 cut(s) 525, 1719
SwaI ATTTAAAT 1 cut(s) 1782
TaaI ACNGT 6 cut(s) 936, 949, 1402, 1819, 1834, 2546
TaiI ACGT 1 cut(s) 577
TaqI TCGA 3 cut(s) 374, 1222, 2431
TatI WGTACW 1 cut(s) 236
TauI GCSGC 2 cut(s) 277, 2345
TfiI GAWTC 6 cut(s) 123, 443, 899, 974, 1257, 2493
Tru1I TTAA 6 cut(s) 110, 1031, 1233, 1781, 1968, 2186
Tru9I TTAA 6 cut(s) 110, 1031, 1233, 1781, 1968, 2186
TscAI CASTG 5 cut(s) 653, 1095, 1407, 2085, 2377
TseFI GTSAC 1 cut(s) 1319
Tsp45I GTSAC 1 cut(s) 1319
TspDTI ATGAA 9 cut(s) 402, 1145, 1659, 1887, 1974, 2051, 2118, 2275, 2331
TspMI CCCGGG 1 cut(s) 334
TspRI CASTG 5 cut(s) 653, 1095, 1407, 2085, 2377
Van91I CCANNNNNTGG 1 cut(s) 200
VpaK11BI GGWCC 4 cut(s) 248, 557, 1292, 1613
XapI RAATTY 3 cut(s) 87, 546, 1985
XbaI TCTAGA 1 cut(s) 1148
XceI RCATGY 3 cut(s) 890, 1079, 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 1499
XhoI CTCGAG 1 cut(s) 1221
XmaI CCCGGG 1 cut(s) 334
XspI CTAG 3 cut(s) 1149, 2568, 2620
Zsp2I ATGCAT 4 cut(s) 892, 1077, 2146, 2193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.