Rorug03G0222300

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005145
Physical Location & Seq
Reverse (-)
20248574 .. 20248933
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0222300.1

Sequence Viewer

Length: 360 bp
ATGCAAACGACGTCGACGTTGCCGAGATCTTCAACCAATGGATCATCAAGTGTCCAACATCAGAGGGTGTACTCAACCGAGGTTCACAAACAAAACCGTTTTGAGATATTCAAAGCCAACTGGGAATATGTCGAGAACTTCAAGAAGATCGGCAACAAGACCTATACCTTAGGTCTGAATAAGTTCTCAGACTTAAGCCCGGAAGAATTCCTGGAAATTTATAGCTGCGGCACAGATCCCACTCCCACCATGCCAGTACCCACTCAAACTCGACCTCCACATATCCTCATGTCGGCATGGATTATCTTGACTATGAGGCATGCTATGAATCAAGGGACTGGACGGAGGATGGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

119

Amino Acids

13.7

Weight (kDa)

9.21

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 19 - 70 2.1e-17 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
AccI GTMKAC 1 cut(s) 14
AciI CCGC 1 cut(s) 228
AclWI GGATC 2 cut(s) 49, 230
AcsI RAATTY 2 cut(s) 206, 216
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 2 cut(s) 71, 258
AfiI CCNNNNNNNGG 1 cut(s) 292
AflII CTTAAG 1 cut(s) 193
AgsI TTSAA 3 cut(s) 33, 112, 142
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 1 cut(s) 225
AluI AGCT 1 cut(s) 225
AlwI GGATC 2 cut(s) 49, 230
ApeKI GCWGC 1 cut(s) 225
ApoI RAATTY 2 cut(s) 206, 216
Asp700I GAANNNNTTC 1 cut(s) 182
AsuC2I CCSGG 1 cut(s) 200
AxyI CCTNAGG 1 cut(s) 169
BbvI GCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 212
BcnI CCSGG 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 193
BglII AGATCT 1 cut(s) 26
BisI GCNGC 2 cut(s) 226, 229
BlsI GCNGC 2 cut(s) 227, 230
Bme1390I CCNGG 2 cut(s) 200, 212
BmrFI CCNGG 2 cut(s) 200, 212
BmrI ACTGGG 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse1I ACTGG 3 cut(s) 125, 254, 343
Bse21I CCTNAGG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 3 cut(s) 125, 254, 343
BseXI GCAGC 1 cut(s) 212
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 1 cut(s) 292
BsmFI GGGAC 1 cut(s) 349
Bsp143I GATC 4 cut(s) 26, 41, 147, 235
BspACI CCGC 1 cut(s) 228
BspCNI CTCAG 1 cut(s) 200
BspPI GGATC 2 cut(s) 49, 230
BspTI CTTAAG 1 cut(s) 193
BsrI ACTGG 3 cut(s) 125, 254, 343
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 4 cut(s) 26, 41, 147, 235
BssNI GRCGYC 1 cut(s) 11
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 11
BstAFI CTTAAG 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 169, 187
BstF5I GGATG 1 cut(s) 354
BstKTI GATC 4 cut(s) 29, 44, 150, 238
BstMBI GATC 4 cut(s) 26, 41, 147, 235
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 198, 210
BstV1I GCAGC 1 cut(s) 212
BstX2I RGATCY 2 cut(s) 26, 235
BstYI RGATCY 2 cut(s) 26, 235
Bsu36I CCTNAGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 321
Csp6I GTAC 2 cut(s) 70, 257
CviAII CATG 4 cut(s) 250, 289, 297, 320
CviJI RGCY 3 cut(s) 116, 198, 225
CviKI_1 RGCY 3 cut(s) 116, 198, 225
CviQI GTAC 2 cut(s) 70, 257
DdeI CTNAG 2 cut(s) 169, 187
DpnI GATC 4 cut(s) 28, 43, 149, 237
DpnII GATC 4 cut(s) 26, 41, 147, 235
Eco81I CCTNAGG 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 210
FaeI CATG 4 cut(s) 253, 292, 300, 323
FaqI GGGAC 1 cut(s) 349
FatI CATG 4 cut(s) 249, 288, 296, 319
FblI GTMKAC 1 cut(s) 14
Fnu4HI GCNGC 2 cut(s) 226, 229
Fsp4HI GCNGC 2 cut(s) 226, 229
GluI GCNGC 2 cut(s) 226, 229
HapII CCGG 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 4 cut(s) 253, 292, 300, 323
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 1 cut(s) 328
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 3 cut(s) 15, 70, 85
Hpy188I TCNGA 3 cut(s) 63, 177, 190
Hpy188III TCNNGA 3 cut(s) 133, 142, 307
Hpy8I GTNNAC 3 cut(s) 15, 70, 85
Hpy99I CGWCG 3 cut(s) 13, 16, 19
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 2 cut(s) 11, 17
HpyCH4V TGCA 1 cut(s) 4
HpyF3I CTNAG 2 cut(s) 169, 187
HpySE526I ACGT 2 cut(s) 11, 17
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 253, 292, 300, 323
Kzo9I GATC 4 cut(s) 26, 41, 147, 235
LpnPI CCDG 6 cut(s) 106, 197, 213, 224, 267, 324
Lsp1109I GCAGC 1 cut(s) 212
MaeII ACGT 2 cut(s) 11, 17
MalI GATC 4 cut(s) 28, 43, 149, 237
MboI GATC 4 cut(s) 26, 41, 147, 235
MboII GAAGA 3 cut(s) 21, 157, 215
MflI RGATCY 2 cut(s) 26, 235
MluCI AATT 2 cut(s) 206, 216
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 6 cut(s) 57, 73, 285, 296, 309, 339
MroXI GAANNNNTTC 1 cut(s) 182
MseI TTAA 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 193
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 200, 212
MvaI CCWGG 1 cut(s) 212
NciI CCSGG 1 cut(s) 200
NdeII GATC 4 cut(s) 26, 41, 147, 235
NlaIII CATG 4 cut(s) 253, 292, 300, 323
NmeAIII GCCGAG 1 cut(s) 48
NspI RCATGY 1 cut(s) 323
PaeI GCATGC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 20
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 1 cut(s) 328
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 2 cut(s) 227, 230
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PsuI RGATCY 2 cut(s) 26, 235
RsaI GTAC 2 cut(s) 71, 258
RsaNI GTAC 2 cut(s) 70, 257
SalI GTCGAC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 194
SatI GCNGC 2 cut(s) 226, 229
Sau3AI GATC 4 cut(s) 26, 41, 147, 235
ScrFI CCNGG 2 cut(s) 200, 212
SetI ASST 8 cut(s) 14, 20, 84, 164, 170, 175, 227, 277
SgrDI CGTCGACG 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SphI GCATGC 1 cut(s) 323
Sse9I AATT 2 cut(s) 206, 216
SsiI CCGC 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 198, 210
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 2 cut(s) 14, 20
TaqI TCGA 3 cut(s) 14, 132, 271
TasI AATT 2 cut(s) 206, 216
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TseI GCWGC 1 cut(s) 225
TspDTI ATGAA 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 358
Vha464I CTTAAG 1 cut(s) 193
XapI RAATTY 2 cut(s) 206, 216
XceI RCATGY 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 14
XmnI GAANNNNTTC 1 cut(s) 182
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.