MD17G1284900.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005145
Physical Location & Seq
Forward (+)
34414585 .. 34420455
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1284900.v1.1.491

Sequence Viewer

Length: 2613 bp
ATGTCAAATCCACCGCAGCCTCCCATTGGATACTCTGTCAGTATCACTCCCTCACGCCCACACATACCATCTCCTGACCCTGAGAAAATTTCTATTCCTCCACCACCTTTAATTACTCCTAGATTCCCTCCACCAAAGTTTCAACACGATCAAACTCCTCCTCCTTTGATCAAAACCCCAAATGTACCATCACCAGCTAATGGGTTGAAAACTGGCAGCCCTATTCCTCACTTGAGTACACCCCCTGGACCTCCTGTTTTTACTTCCCCTGTGCGGCCTGCTGCTGTGCCTTTTCGTGCTTCACCTGCAACTCCTCAGCCAGTTGCTTTCTCCCCGGGTACATCTTTGTCAACATGTTCGCCTCCCAATTTTTCGAATGGCTCACAGCACCAACTTTCTAATGATACAGAGGATGATGCTGTGCCTGTTGGAGAATCACCATATGTCCTATTCTCAGCGCATAAGGTGTTGAAACAAAAGAAACAAGCAAATGTACCCAGTTTGGGTTTTGGGGCCTTGGTTTCACCTGGAAGGGAAATTTCTCCAGGTCCCCAGATAATACAACGTGATCCTCATCGCTGCCACAGTTGTGGAGCTTATGCCAATATTTATTGCAACATCTTATTGGGCTCAGGTCAGTGGCAATGTGTAATTTGCCGAGAACTGAATGGAAGCGAGGGCGAATACAGAGCTCCCAGCAAGGAAGATCTTTGTAATTTTCCAGAGTTGTCATCTCCTATGGTTGATTATGTTCAAACTGGGAACAATAGACCCGGTTTTGTTCCCGTTTCTGACTCAAGAATGTCTGCACCTATAGTTCTTGTCATAGATGAGTGTTTAGACGAACCACATCTGTGGGACTTACAGAGCTCCTTGCATGCATTTGTTGATTCACTTCCCCCGACAATGAGAATTGGAATAATACTGTATGGTCGCACAGTATCAGTTTATGATTTTTCAGAGGAATCAATTGCATCTGCTGATGTGCTTCCAGGAGATAAATCACCAAGTCAGGACGCCTTGAAGGCTTTGATATATGGAAGTGGTTTATACTTGTCTCCAATGCATGCATCATTACCAGTAGCACATGCCATATTCTCATCCTTGAGGCCATACAAACTGAAAATTTCAGAAACTTCTAGAGATCGGTGCCTGGGTACGGCAGTTGAGGTTGCACTTGCTATAGTTCAAGGGCCATCAGGAGAAATGTCTCGCGGGGTAATTAAAAGGTCTGGGGGTAATAGCAGAATCATTGTTTGTGCTGGTGGGCCTAATACATACGGTCCTGGATCTGTTCCTCATTCTTTCAGTCACCCAAATTATCCTCATATGGAAAAGACTGCATTGAAATGGATGGAGCATCTGGGTCAAGAGGCACACCGACAAAATACAGTGGTCGACATTTTATGTGCTGGGCAATGCCCTGTAAGAGTTCCTGTTTTGCAGCCTCTTGCAAAAGCTTCTGGAGGCATTTTTGTTCTCCATGACGACTTTGGGGAAGCCTTTGGTGTGAACTTACAAAGGGCATCTACCAGGGCTGCAGGCTCCCGTGGGTTCTTGGCAATACGCTGTTCCGATGACATTCTCATAACTCAAGTTGTGGGTCCTGGTGAAGAGGCACATATTGACACTCATGAAACCTTCAAAAACGACACTTCCCTTTATATACAAATGCTTAGTGTTGAGGAGACACAGAGCTTCTCACTCTCCTTGGAAAATAAGAGGGACATTAGGACTGAGTATGTGTATTTCCAGTTTACAATTCAGTACTTAAATGTGTATCAAGCTGATATATCAAGAGTAATTACTGTTAGATTGCCAACAGTTGATAGTGTTTCAGCATATCTTACAAGTGTTCAAGATGAAGTAGCAGCAGTTCTTATTGCAAAACGGACTCTCTTGCGAGCTAAAAACTATTCTGATGCAATTGATATGCGAGCAACAATAGATGAAAGAATTAAAGACATTGCTCTGAAATTTGGGTCTCAAGTACCAAAGTCAAAGTTTTATCGGTTTCCAAAGGAGATCTCTTTATTGCCAGAGCTCCTGTTTCAGCTAAGAAGGGGCCCACTGTTGGGAAGCATTGTTGGCCATGAAGATGAGAGGTCTGTGTTACGAAACTTGTTTCTGAATGCATCCTTTGATCTCTCACTTCGAATGGTAGCACCTCGCTGTTTAATGCATCGGGAAGGGGGAACATTTGAGGAACTACCAGCTTATGACCTTGCTATGCAGTCAGATGCAGCAGTTGTTCTTGACCATGGTACAGATGTCTTCATTTGGTTGGGTGCTGAACTCGCTGCTGATGAAGGAAAAAGTGCAGCTGCTTTAGCAGCTTGCAGAACATTAGCTGAAGAGCTCACTGAACTGCGGTTTCCAGCTCCTCGGATCCTGTCGTTCAAGGAAGGAAGTTCTCAGGCTCGATATTTTGTATCGCGGCTCATACCTGCACACAAGGATCCTCCTTATGAGCAGGAGGCAAGATTCCCACAGCTACGAACTTTGACAACAGAAAAGCGGACAAAGCTGAAAAGCAGTTTTATTAGTTTTGACGAGCCTAGTTTCTGCGAGTGGGTACGAAGCTTGAAAGTGGTGCCTCCGGAACCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

871

Amino Acids

95.23

Weight (kDa)

6.38

Isoelectric Point (pI)

54.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 191 - 229 1.9e-10 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 273 - 499 1.7e-12 Sec23/Sec24 trunk domain
Sec23_BS PF08033 518 - 608 7.3e-12 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 622 - 718 4.7e-13 Sec23/Sec24 helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 313
Acc36I ACCTGC 2 cut(s) 313, 2455
AccB1I GGYRCC 2 cut(s) 1149, 2593
AccB7I CCANNNNNTGG 1 cut(s) 200
AccI GTMKAC 1 cut(s) 1398
AccII CGCG 2 cut(s) 1215, 2437
AccIII TCCGGA 1 cut(s) 2599
AciI CCGC 6 cut(s) 14, 274, 1215, 2371, 2437, 2518
AclWI GGATC 6 cut(s) 563, 1297, 2383, 2396, 2453, 2466
AcoI YGGCCR 1 cut(s) 2089
AcsI RAATTY 4 cut(s) 87, 537, 1125, 1976
AcuI CTGAAG 1 cut(s) 2373
AcyI GRCGYC 1 cut(s) 1017
AfaI GTAC 9 cut(s) 186, 238, 340, 495, 1159, 1769, 1992, 2266, 2577
AfiI CCNNNNNNNGG 7 cut(s) 26, 200, 273, 503, 1159, 2074, 2075
AflIII ACRYGT 1 cut(s) 353
AjnI CCWGG 8 cut(s) 244, 526, 544, 991, 1152, 1285, 1532, 1606
AleI CACNNNNGTG 2 cut(s) 588, 853
Alw21I GWGCWC 4 cut(s) 694, 872, 2046, 2361
Alw26I GTCTC 4 cut(s) 1062, 1215, 1682, 1989
AlwI GGATC 6 cut(s) 563, 1297, 2383, 2396, 2453, 2466
Ama87I CYCGRG 1 cut(s) 334
Aor13HI TCCGGA 1 cut(s) 2599
AoxI GGCC 7 cut(s) 275, 513, 1109, 1193, 1268, 2065, 2089
ApaI GGGCCC 1 cut(s) 2069
ApoI RAATTY 4 cut(s) 87, 537, 1125, 1976
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 9 cut(s) 248, 513, 548, 1193, 1268, 1283, 1604, 2065, 2066
AsuC2I CCSGG 3 cut(s) 335, 336, 774
AsuHPI GGTGA 7 cut(s) 183, 294, 429, 516, 996, 1304, 1622
AsuII TTCGAA 2 cut(s) 374, 2155
AvaI CYCGRG 1 cut(s) 334
AvaII GGWCC 4 cut(s) 248, 548, 1283, 1604
BaeGI GKGCMC 1 cut(s) 2069
BaeI ACNNNNGTAYC 4 cut(s) 1149, 1182, 2256, 2289
BalI TGGCCA 1 cut(s) 2091
BamHI GGATCC 2 cut(s) 2388, 2458
BanI GGYRCC 2 cut(s) 1149, 2593
BanII GRGCYC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
BbsI GAAGAC 1 cut(s) 2266
Bbv12I GWGCWC 4 cut(s) 694, 872, 2046, 2361
BbvCI CCTCAGC 1 cut(s) 315
BccI CCATC 4 cut(s) 76, 196, 1204, 1348
BceAI ACGGC 1 cut(s) 1176
BciT130I CCWGG 8 cut(s) 246, 528, 546, 993, 1154, 1287, 1534, 1608
BciVI GTATCC 1 cut(s) 23
BclI TGATCA 1 cut(s) 168
BcnI CCSGG 3 cut(s) 335, 336, 774
BcoDI GTCTC 4 cut(s) 1062, 1215, 1682, 1989
BfaI CTAG 4 cut(s) 120, 1140, 2559, 2611
BfmI CTRYAG 3 cut(s) 813, 1182, 1539
BfuAI ACCTGC 2 cut(s) 313, 2455
BfuI GTATCC 1 cut(s) 23
BglI GCCNNNNNGGC 1 cut(s) 1025
BglII AGATCT 2 cut(s) 706, 2025
BmcAI AGTACT 1 cut(s) 1769
Bme18I GGWCC 4 cut(s) 248, 548, 1283, 1604
BmeT110I CYCGRG 1 cut(s) 334
BmgT120I GGNCC 9 cut(s) 248, 513, 548, 1193, 1268, 1283, 1604, 2065, 2066
BmrI ACTGGG 2 cut(s) 492, 768
BmsI GCATC 9 cut(s) 406, 983, 1079, 1369, 1535, 1912, 2144, 2191, 2230
BmuI ACTGGG 2 cut(s) 492, 768
BpiI GAAGAC 1 cut(s) 2266
BpmI CTGGAG 2 cut(s) 528, 1485
Bpu10I CCTNAGC 2 cut(s) 315, 631
Bpu14I TTCGAA 2 cut(s) 374, 2155
BpuEI CTTGAG 5 cut(s) 253, 781, 1126, 1578, 1971
BpuMI CCSGG 3 cut(s) 335, 336, 774
BsaBI GATNNNNATC 1 cut(s) 573
BsaHI GRCGYC 1 cut(s) 1017
BsaI GGTCTC 1 cut(s) 1989
BsaWI WCCGGW 1 cut(s) 2599
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 200, 273, 503, 1159, 2074, 2075
Bse1I ACTGG 6 cut(s) 217, 320, 498, 763, 1079, 1753
Bse3DI GCAATG 3 cut(s) 650, 1424, 1965
Bse8I GATNNNNATC 1 cut(s) 573
BseAI TCCGGA 1 cut(s) 2599
BseBI CCWGG 8 cut(s) 246, 528, 546, 993, 1154, 1287, 1534, 1608
BseGI GGATG 4 cut(s) 418, 1100, 1359, 2135
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 7 cut(s) 26, 200, 273, 503, 1159, 2074, 2075
BseMI GCAATG 3 cut(s) 650, 1424, 1965
BseMII CTCAG 6 cut(s) 72, 329, 468, 645, 1728, 2429
BseNI ACTGG 6 cut(s) 217, 320, 498, 763, 1079, 1753
BseRI GAGGAG 5 cut(s) 147, 150, 303, 1700, 2373
BseSI GKGCMC 1 cut(s) 2069
BseYI CCCAGC 2 cut(s) 695, 1412
BsgI GTGCAG 3 cut(s) 792, 2340, 2433
Bsh1236I CGCG 2 cut(s) 1215, 2437
BshFI GGCC 7 cut(s) 277, 515, 1111, 1195, 1270, 2067, 2091
BshNI GGYRCC 2 cut(s) 1149, 2593
BsiHKAI GWGCWC 4 cut(s) 694, 872, 2046, 2361
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 3 cut(s) 335, 774, 2600
BslFI GGGAC 3 cut(s) 534, 872, 1739
BslI CCNNNNNNNGG 7 cut(s) 26, 200, 273, 503, 1159, 2074, 2075
BsmAI GTCTC 4 cut(s) 1062, 1215, 1682, 1989
BsmFI GGGAC 3 cut(s) 534, 872, 1739
BsmI GAATGC 1 cut(s) 2137
BsnI GGCC 7 cut(s) 277, 515, 1111, 1195, 1270, 2067, 2091
Bso31I GGTCTC 1 cut(s) 1989
BsoBI CYCGRG 1 cut(s) 334
Bsp119I TTCGAA 2 cut(s) 374, 2155
Bsp120I GGGCCC 1 cut(s) 2065
Bsp1286I GDGCHC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
Bsp13I TCCGGA 1 cut(s) 2599
Bsp19I CCATGG 1 cut(s) 2260
BspACI CCGC 6 cut(s) 14, 274, 1215, 2371, 2437, 2518
BspANI GGCC 7 cut(s) 277, 515, 1111, 1195, 1270, 2067, 2091
BspCNI CTCAG 6 cut(s) 73, 328, 467, 644, 1729, 2428
BspEI TCCGGA 1 cut(s) 2599
BspFNI CGCG 2 cut(s) 1215, 2437
BspHI TCATGA 1 cut(s) 1633
BspMAI CTGCAG 1 cut(s) 1543
BspMI ACCTGC 2 cut(s) 313, 2455
BspPI GGATC 6 cut(s) 563, 1297, 2383, 2396, 2453, 2466
BspQI GCTCTTC 1 cut(s) 2349
BspT104I TTCGAA 2 cut(s) 374, 2155
BspT107I GGYRCC 2 cut(s) 1149, 2593
BspTNI GGTCTC 1 cut(s) 1989
BsrDI GCAATG 3 cut(s) 650, 1424, 1965
BsrI ACTGG 6 cut(s) 217, 320, 498, 763, 1079, 1753
BssNI GRCGYC 1 cut(s) 1017
BssT1I CCWWGG 3 cut(s) 516, 1710, 2260
Bst2UI CCWGG 8 cut(s) 246, 528, 546, 993, 1154, 1287, 1534, 1608
Bst4CI ACNGT 8 cut(s) 587, 927, 940, 1283, 1393, 1810, 1825, 2073
Bst6I CTCTTC 2 cut(s) 1608, 2349
BstACI GRCGYC 1 cut(s) 1017
BstBI TTCGAA 2 cut(s) 374, 2155
BstC8I GCNNGC 7 cut(s) 279, 879, 1068, 1543, 1905, 1938, 2338
BstDEI CTNAG 8 cut(s) 81, 315, 454, 631, 1676, 1737, 2057, 2415
BstDSI CCRYGG 2 cut(s) 1549, 2260
BstF5I GGATG 4 cut(s) 418, 1100, 1359, 2135
BstFNI CGCG 2 cut(s) 1215, 2437
BstHHI GCGC 1 cut(s) 460
BstMAI GTCTC 4 cut(s) 1062, 1215, 1682, 1989
BstMWI GCNNNNNNNGC 7 cut(s) 305, 1025, 2088, 2297, 2330, 2333, 2524
BstNI CCWGG 8 cut(s) 246, 528, 546, 993, 1154, 1287, 1534, 1608
BstNSI RCATGY 4 cut(s) 357, 881, 1070, 1091
BstSFI CTRYAG 3 cut(s) 813, 1182, 1539
BstSLI GKGCMC 1 cut(s) 2069
BstUI CGCG 2 cut(s) 1215, 2437
BstV2I GAAGAC 1 cut(s) 2266
BstX2I RGATCY 5 cut(s) 706, 1289, 2025, 2388, 2458
BstXI CCANNNNNNTGG 2 cut(s) 590, 855
BstYI RGATCY 5 cut(s) 706, 1289, 2025, 2388, 2458
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 7 cut(s) 277, 515, 1111, 1195, 1270, 2067, 2091
BtgI CCRYGG 2 cut(s) 1549, 2260
BtgZI GCGATG 1 cut(s) 560
BtsCI GGATG 4 cut(s) 418, 1100, 1359, 2135
BtsIMutI CAGTG 4 cut(s) 644, 1398, 2069, 2361
BveI ACCTGC 2 cut(s) 313, 2455
Cac8I GCNNGC 7 cut(s) 279, 879, 1068, 1543, 1905, 1938, 2338
CciI TCATGA 1 cut(s) 1633
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 9 cut(s) 248, 513, 548, 1193, 1268, 1283, 1604, 2065, 2066
Cfr9I CCCGGG 1 cut(s) 334
CseI GACGC 1 cut(s) 1025
Csp6I GTAC 9 cut(s) 185, 237, 339, 494, 1158, 1768, 1991, 2265, 2576
CviAII CATG 8 cut(s) 354, 878, 1067, 1088, 1484, 1634, 2093, 2261
CviQI GTAC 9 cut(s) 185, 237, 339, 494, 1158, 1768, 1991, 2265, 2576
DdeI CTNAG 8 cut(s) 81, 315, 454, 631, 1676, 1737, 2057, 2415
EaeI YGGCCR 1 cut(s) 2089
Eam1104I CTCTTC 2 cut(s) 1608, 2349
EarI CTCTTC 2 cut(s) 1608, 2349
Ecl136II GAGCTC 4 cut(s) 692, 870, 2044, 2359
Eco130I CCWWGG 3 cut(s) 516, 1710, 2260
Eco24I GRGCYC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
Eco31I GGTCTC 1 cut(s) 1989
Eco47I GGWCC 4 cut(s) 248, 548, 1283, 1604
Eco53kI GAGCTC 4 cut(s) 692, 870, 2044, 2359
Eco57I CTGAAG 1 cut(s) 2373
Eco88I CYCGRG 1 cut(s) 334
EcoICRI GAGCTC 4 cut(s) 692, 870, 2044, 2359
EcoO109I RGGNCCY 4 cut(s) 513, 548, 1604, 2065
EcoRII CCWGG 8 cut(s) 244, 526, 544, 991, 1152, 1285, 1532, 1606
EcoT14I CCWWGG 3 cut(s) 516, 1710, 2260
EcoT22I ATGCAT 5 cut(s) 883, 1068, 1072, 2137, 2184
EcoT38I GRGCYC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
ErhI CCWWGG 3 cut(s) 516, 1710, 2260
FaeI CATG 8 cut(s) 357, 881, 1070, 1091, 1487, 1637, 2096, 2264
FaqI GGGAC 3 cut(s) 534, 872, 1739
FatI CATG 8 cut(s) 353, 877, 1066, 1087, 1483, 1633, 2092, 2260
FauI CCCGC 1 cut(s) 1208
FauNDI CATATG 2 cut(s) 442, 1329
FbaI TGATCA 1 cut(s) 168
FblI GTMKAC 1 cut(s) 1398
FokI GGATG 4 cut(s) 425, 1087, 1366, 2122
FriOI GRGCYC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
FspBI CTAG 4 cut(s) 120, 1140, 2559, 2611
GlaI GCGC 1 cut(s) 459
GsaI CCCAGC 2 cut(s) 699, 1416
GsuI CTGGAG 2 cut(s) 528, 1485
HaeIII GGCC 7 cut(s) 277, 515, 1111, 1195, 1270, 2067, 2091
HapII CCGG 3 cut(s) 335, 774, 2600
HgaI GACGC 1 cut(s) 1025
HhaI GCGC 1 cut(s) 460
Hin1I GRCGYC 1 cut(s) 1017
Hin1II CATG 8 cut(s) 357, 881, 1070, 1091, 1487, 1637, 2096, 2264
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 2 cut(s) 351, 1399
HindII GTYRAC 2 cut(s) 351, 1399
HindIII AAGCTT 2 cut(s) 1458, 2581
HinfI GANTC 8 cut(s) 123, 434, 794, 890, 965, 1248, 1894, 2484
HpaII CCGG 3 cut(s) 335, 774, 2600
HphI GGTGA 7 cut(s) 183, 294, 429, 516, 996, 1304, 1622
Hpy166II GTNNAC 5 cut(s) 239, 351, 1399, 1513, 1758
Hpy188I TCNGA 9 cut(s) 793, 961, 1132, 1576, 1921, 1974, 2130, 2239, 2388
Hpy8I GTNNAC 5 cut(s) 239, 351, 1399, 1513, 1758
HpyAV CCTTC 7 cut(s) 525, 1018, 1651, 2055, 2183, 2303, 2399
HpyCH4III ACNGT 8 cut(s) 587, 927, 940, 1283, 1393, 1810, 1825, 2073
HpyCH4IV ACGT 1 cut(s) 565
HpyF10VI GCNNNNNNNGC 7 cut(s) 305, 1025, 2088, 2297, 2330, 2333, 2524
HpyF3I CTNAG 8 cut(s) 81, 315, 454, 631, 1676, 1737, 2057, 2415
HpySE526I ACGT 1 cut(s) 565
Hsp92I GRCGYC 1 cut(s) 1017
Hsp92II CATG 8 cut(s) 357, 881, 1070, 1091, 1487, 1637, 2096, 2264
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 2599
Ksp22I TGATCA 1 cut(s) 168
LguI GCTCTTC 1 cut(s) 2349
LmnI GCTCC 7 cut(s) 593, 697, 875, 1357, 1550, 2049, 2386
LweI GCATC 9 cut(s) 406, 983, 1079, 1369, 1535, 1912, 2144, 2191, 2230
MaeI CTAG 4 cut(s) 120, 1140, 2559, 2611
MaeII ACGT 1 cut(s) 565
MaeIII GTNAC 2 cut(s) 1310, 2112
MboII GAAGA 5 cut(s) 716, 1625, 2108, 2266, 2366
MfeI CAATTG 2 cut(s) 969, 1926
MflI RGATCY 5 cut(s) 706, 1289, 2025, 2388, 2458
MhlI GDGCHC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
MlsI TGGCCA 1 cut(s) 2091
MluNI TGGCCA 1 cut(s) 2091
MlyI GAGTC 2 cut(s) 788, 1888
MmeI TCCRAC 1 cut(s) 409
Mox20I TGGCCA 1 cut(s) 2091
Mph1103I ATGCAT 5 cut(s) 883, 1068, 1072, 2137, 2184
MroI TCCGGA 1 cut(s) 2599
MscI TGGCCA 1 cut(s) 2091
MseI TTAA 5 cut(s) 110, 1224, 1772, 1959, 2177
MslI CAYNNNNRTG 4 cut(s) 588, 853, 1348, 2097
Msp20I TGGCCA 1 cut(s) 2091
MspA1I CMGCKG 1 cut(s) 2324
MspI CCGG 3 cut(s) 335, 774, 2600
MunI CAATTG 2 cut(s) 969, 1926
Mva1269I GAATGC 1 cut(s) 2137
MvaI CCWGG 8 cut(s) 246, 528, 546, 993, 1154, 1287, 1534, 1608
MvnI CGCG 2 cut(s) 1215, 2437
MwoI GCNNNNNNNGC 7 cut(s) 305, 1025, 2088, 2297, 2330, 2333, 2524
NciI CCSGG 3 cut(s) 335, 336, 774
NcoI CCATGG 1 cut(s) 2260
NdeI CATATG 2 cut(s) 442, 1329
NlaIII CATG 8 cut(s) 357, 881, 1070, 1091, 1487, 1637, 2096, 2264
NmeAIII GCCGAG 1 cut(s) 683
NmuCI GTSAC 1 cut(s) 1310
NsiI ATGCAT 5 cut(s) 883, 1068, 1072, 2137, 2184
NspI RCATGY 4 cut(s) 357, 881, 1070, 1091
NspV TTCGAA 2 cut(s) 374, 2155
OliI CACNNNNGTG 2 cut(s) 588, 853
PaeI GCATGC 2 cut(s) 881, 1070
PagI TCATGA 1 cut(s) 1633
PaqCI CACCTGC 1 cut(s) 313
PciI ACATGT 1 cut(s) 353
PciSI GCTCTTC 1 cut(s) 2349
PctI GAATGC 1 cut(s) 2137
PfeI GAWTC 6 cut(s) 123, 434, 890, 965, 1248, 2484
PflMI CCANNNNNTGG 1 cut(s) 200
PfoI TCCNGGA 2 cut(s) 991, 1285
PleI GAGTC 2 cut(s) 788, 1888
PpsI GAGTC 2 cut(s) 788, 1888
PpuMI RGGWCCY 2 cut(s) 548, 1604
PscI ACATGT 1 cut(s) 353
Psp124BI GAGCTC 4 cut(s) 694, 872, 2046, 2361
Psp5II RGGWCCY 2 cut(s) 548, 1604
Psp6I CCWGG 8 cut(s) 244, 526, 544, 991, 1152, 1285, 1532, 1606
PspFI CCCAGC 2 cut(s) 695, 1412
PspGI CCWGG 8 cut(s) 244, 526, 544, 991, 1152, 1285, 1532, 1606
PspOMI GGGCCC 1 cut(s) 2065
PspPI GGNCC 9 cut(s) 248, 513, 548, 1193, 1268, 1283, 1604, 2065, 2066
PspPPI RGGWCCY 2 cut(s) 548, 1604
PsrI GAACNNNNNNTAC 2 cut(s) 1860, 1892
PstI CTGCAG 1 cut(s) 1543
PsuI RGATCY 5 cut(s) 706, 1289, 2025, 2388, 2458
PvuII CAGCTG 1 cut(s) 2324
RsaI GTAC 9 cut(s) 186, 238, 340, 495, 1159, 1769, 1992, 2266, 2577
RsaNI GTAC 9 cut(s) 185, 237, 339, 494, 1158, 1768, 1991, 2265, 2576
RseI CAYNNNNRTG 4 cut(s) 588, 853, 1348, 2097
SacI GAGCTC 4 cut(s) 694, 872, 2046, 2361
SalI GTCGAC 1 cut(s) 1397
SapI GCTCTTC 1 cut(s) 2349
SaqAI TTAA 5 cut(s) 110, 1224, 1772, 1959, 2177
Sau96I GGNCC 9 cut(s) 248, 513, 548, 1193, 1268, 1283, 1604, 2065, 2066
ScaI AGTACT 1 cut(s) 1769
SchI GAGTC 2 cut(s) 788, 1888
SduI GDGCHC 6 cut(s) 632, 694, 872, 2046, 2069, 2361
SfaNI GCATC 9 cut(s) 406, 983, 1079, 1369, 1535, 1912, 2144, 2191, 2230
SfcI CTRYAG 3 cut(s) 813, 1182, 1539
SfuI TTCGAA 2 cut(s) 374, 2155
SinI GGWCC 4 cut(s) 248, 548, 1283, 1604
SmaI CCCGGG 1 cut(s) 336
SmiMI CAYNNNNRTG 4 cut(s) 588, 853, 1348, 2097
SmlI CTYRAG 5 cut(s) 232, 796, 1105, 1593, 1986
SmoI CTYRAG 5 cut(s) 232, 796, 1105, 1593, 1986
SphI GCATGC 2 cut(s) 881, 1070
SsiI CCGC 6 cut(s) 14, 274, 1215, 2371, 2437, 2518
SspI AATATT 1 cut(s) 607
SspMI CTAG 4 cut(s) 120, 1140, 2559, 2611
SstI GAGCTC 4 cut(s) 694, 872, 2046, 2361
StyI CCWWGG 3 cut(s) 516, 1710, 2260
TaaI ACNGT 8 cut(s) 587, 927, 940, 1283, 1393, 1810, 1825, 2073
TaiI ACGT 1 cut(s) 568
TaqI TCGA 4 cut(s) 374, 1398, 2155, 2422
TatI WGTACW 2 cut(s) 236, 1767
TauI GCSGC 2 cut(s) 277, 2440
TfiI GAWTC 6 cut(s) 123, 434, 890, 965, 1248, 2484
Tru1I TTAA 5 cut(s) 110, 1224, 1772, 1959, 2177
Tru9I TTAA 5 cut(s) 110, 1224, 1772, 1959, 2177
TscAI CASTG 4 cut(s) 644, 1398, 2076, 2368
TseFI GTSAC 1 cut(s) 1310
Tsp45I GTSAC 1 cut(s) 1310
TspDTI ATGAA 6 cut(s) 1650, 1878, 1965, 2109, 2266, 2322
TspGWI ACGGA 1 cut(s) 1906
TspMI CCCGGG 1 cut(s) 334
TspRI CASTG 4 cut(s) 644, 1398, 2076, 2368
Van91I CCANNNNNTGG 1 cut(s) 200
VpaK11BI GGWCC 4 cut(s) 248, 548, 1283, 1604
XapI RAATTY 4 cut(s) 87, 537, 1125, 1976
XbaI TCTAGA 1 cut(s) 1139
XceI RCATGY 4 cut(s) 357, 881, 1070, 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 1490
XmaI CCCGGG 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 1398
XspI CTAG 4 cut(s) 120, 1140, 2559, 2611
ZrmI AGTACT 1 cut(s) 1769
Zsp2I ATGCAT 5 cut(s) 883, 1068, 1072, 2137, 2184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.