Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0005145

Physical Location & Seq

Genomic Coordinates Reverse (-)

31753217 .. 31758994

Gene Structure 5778 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG301400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGTTGATTATGTTCAAACTGCGAACAATAGACCTGGTTTTGTTCCAGTTTCTGATTCTAGAATGTCTGCACCCCTTGTTCTTGTTATAGACGAGTGTTTAGATGAACCACATCTCTGGCATTTACAGAGCTCCTTGCATGCCTTTGTCGATTCACTTCCCCCTACAACGAGAATTGGAATAGTACTGTATGGTCGCACAGTATCAGTTTATGATTTTTCAGAGGAATCAGTTGCATCTGCTGATGTGCTTCCAGGCGACAAATCACCATGCCAGGAGTCCTTGAAGGCATTGATATATGGAACCGGTATATATTTGTCTCCAATGCATGCTTCACTGCCTGTAGCACATGCCATATTCTCATCATTAAGGCCGTATAAATTGAACATTCCAGAAGCTTCTAGAGACCGGTGCCTTGGCACAGCAGTTGAGGTTGCTCTTGCTATAATTCAAGGCCCATCAGCAGACATTTCTCGAGGGGTAATAAAAAGGTCAGGAGGTAATAGCAGAGTCATAGTTTGTGCTGGTGGACCCAATACTTATGGTCCTGGATCTGTTCCTCATTCTTTCAGTCACCCAAATTATGCTCACTTGGAAAAGACCGCATTGAAATGGATGGAGCATCTGGGTCAAGAGGCACACCGACACAATACAGTAGTTGATATTCTTTGCGCTGGGCAATGCCCTGTAAGGGTTCCTGTTTTGCAGCCTCTTGCAAAAGCTTCTGGGGGCGTTTTCGTTCTCCATGATGACTTTGGTGAAGCCTTTGGAGTGAACTTGCAAAGGGCATCCATCAGGGCAGCAGGCTTCCGTGGGTTCTTGGCAATTCGCTGTTCAGATGATATTCTCATAACCCAAGTTGTGGGTCCTGGTGAAGAGGCACATGTAGACACTCATGAAACGTTCAAAAATGACACATCTCTTTACATACAAATGCCAAGTGTCGAAGAAACACAGAGCTTCTCACTCTCCTTGGAAAATAAGAGGGACATTAGGACTGAGTATGTATATTTCCAGTTTACAACTCAGTTTCTGAATGTTTATCAAGCTGATATATCAAGAGTAATAACTGTTAGATTGCCAACAGTTGATAGTGTTTCAGCATATCTTGAGAGTGTTCAAGAGGAAGTGGCAGCAGTTCTTATTGCAAAGAGGACTCTCTTGCGAGCCAAAAACAGTTCTGATGCAATTGATATGCAATCAACAATAGATGAAAGAATTAAAGACATTGCTTTAAAATATGGTTCTCAAGTACCAAAATCAAAGCAATATCGTTTCCCAAAGGAGATGTCTTTATTACCAGAGCACCTGTTTCATCTTAGAAGGGGCCCACTTTTGGGAAACATTGTTGGCCATGAAGATGAGAGGTCTGTGTTACGGAACTTGTTTCTGAATGCATCCTTTGATCTGTCTCTGCGAATGGTTGCGCCTCGTTGTCTAATGCATCGGGAAGGGGGTACTTTTGAGGAACTACCAGCTTATGACCTTGCTATGCAGTCTGATGCAGCAGTTGTACTTGATCATGGCACAGATGTCTTCATTTGGTTGGGTGCTGAACTTGCTGCTGATGAAGGGAAAAGTGCAGCTGCTTTAGCTGCGTGCAGAACATTGGCTGAAGAGCTCAGTGAACTTCGGTTTCCAGCCCCTCGGATTCTTTCCTTCAAGGAAGGAAGTTCTCAGGCTCGGTATTTTGTATCACGGCTCATTCCTGCGCACAAGGATCCTCCTTATGAGCAGGAGGCAAGATTTCCTCAGCTACGAACTTTGACGATAGAACGGCGCACAAAGCTGAAAAGCAGTTTTATTAGTTTTGATGATCCTAGCTTCTGTGAGTGGGTACGAAGCTTGAGAGTGGTTCCCCCAGAACCAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

624

Amino Acids

69.09

Weight (kDa)

6.01

Isoelectric Point (pI)

51.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec23_trunk	PF04811	27 - 253	1.1e-12	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	269 - 363	3.9e-13	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	376 - 472	1.4e-15	Sec23/Sec24 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005145)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01810 AT4G01810 AT4G01810
fragaria_vesca	FvH4_1g26250 FvH4_1g26250 FvH4_1g26250 FvH4_1g26250 FvH4_1g26250
malus_domestica	MD09G1290300.v1.1 MD17G1284900.v1.1
prunus_persica	Prupe.3G002800_v2.0.a1 Prupe.3G002800_v2.0.a1 Prupe.3G002800_v2.0.a1 Prupe.3G002800_v2.0.a1
pyrus_communis	pycom09g19810 pycom17g28070
rosa_chinensis	RchiOBHm_Chr3g0485911
rosa_laevigata	RLG00000023123
rosa_multiflora	Rmu_sc0004876.1_g000006 Rmu_sc0012870.1_g000011
rosa_roxburghii	Rroxscaffold_6G00396210
rosa_rugosa	Rorug03G0222000 Rorug03G0222100 Rorug03G0222200 Rorug03G0222300
rosa_samantha	Rh3AG271700 Rh3BG306700 Rh3DG301400
rosa_wichuraiana	Rw0G015510 Rw3G024140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1713
AccB1I	GGYRCC	1 cut(s)	411
AccB7I	CCANNNNNTGG	1 cut(s)	862
AccI	GTMKAC	1 cut(s)	888
AciI	CCGC	1 cut(s)	603
AclI	AACGTT	1 cut(s)	902
AclWI	GGATC	4 cut(s)	559, 1715, 1728, 1811
AcoI	YGGCCR	1 cut(s)	1351
AcuI	CTGAAG	1 cut(s)	1635
AfaI	GTAC	5 cut(s)	186, 1254, 1459, 1515, 1839
AfiI	CCNNNNNNNGG	4 cut(s)	691, 862, 1336, 1337
AflIII	ACRYGT	1 cut(s)	883
AgeI	ACCGGT	2 cut(s)	305, 408
AgsI	TTSAA	8 cut(s)	17, 286, 385, 452, 610, 907, 1121, 1663
AjnI	CCWGG	5 cut(s)	34, 253, 273, 547, 868
AjuI	GAANNNNNNNTTGG	2 cut(s)	316, 348
Alw21I	GWGCWC	3 cut(s)	134, 1308, 1623
Alw26I	GTCTC	3 cut(s)	324, 399, 1416
AlwI	GGATC	4 cut(s)	559, 1715, 1728, 1811
AlwNI	CAGNNNCTG	2 cut(s)	53, 1033
Ama87I	CYCGRG	1 cut(s)	474
AoxI	GGCC	4 cut(s)	371, 454, 1327, 1351
ApaI	GGGCCC	1 cut(s)	1331
ApeKI	GCWGC	8 cut(s)	706, 800, 1133, 1505, 1562, 1583, 1586, 1595
ArsI	GACNNNNNNTTYG	2 cut(s)	1274, 1306
AsiGI	ACCGGT	2 cut(s)	305, 408
AspLEI	GCGC	4 cut(s)	674, 1429, 1714, 1782
AspS9I	GGNCC	6 cut(s)	455, 530, 545, 866, 1327, 1328
AsuHPI	GGTGA	4 cut(s)	258, 566, 770, 884
AvaI	CYCGRG	1 cut(s)	474
AvaII	GGWCC	3 cut(s)	530, 545, 866
BaeGI	GKGCMC	1 cut(s)	1331
BalI	TGGCCA	1 cut(s)	1353
BamHI	GGATCC	1 cut(s)	1720
BanI	GGYRCC	1 cut(s)	411
BanII	GRGCYC	3 cut(s)	134, 1331, 1623
BbsI	GAAGAC	1 cut(s)	1528
Bbv12I	GWGCWC	3 cut(s)	134, 1308, 1623
BbvCI	CCTCAGC	1 cut(s)	1752
BbvI	GCAGC	8 cut(s)	718, 812, 1145, 1517, 1549, 1573, 1582, 1595
BccI	CCATC	3 cut(s)	466, 610, 800
BceAI	ACGGC	3 cut(s)	358, 1715, 1793
BciT130I	CCWGG	5 cut(s)	36, 255, 275, 549, 870
BclI	TGATCA	1 cut(s)	1519
BcoDI	GTCTC	3 cut(s)	324, 399, 1416
BfaI	CTAG	3 cut(s)	60, 402, 1821
BfmI	CTRYAG	1 cut(s)	342
BisI	GCNGC	8 cut(s)	707, 801, 1134, 1506, 1563, 1584, 1587, 1596
BlsI	GCNGC	8 cut(s)	708, 802, 1135, 1507, 1564, 1585, 1588, 1597
BmcAI	AGTACT	1 cut(s)	186
Bme1390I	CCNGG	5 cut(s)	36, 255, 275, 549, 870
Bme18I	GGWCC	3 cut(s)	530, 545, 866
BmeT110I	CYCGRG	1 cut(s)	474
BmgT120I	GGNCC	6 cut(s)	455, 530, 545, 866, 1327, 1328
BmiI	GGNNCC	9 cut(s)	304, 413, 532, 696, 867, 1328, 1329, 1722, 1857
BmrFI	CCNGG	5 cut(s)	36, 255, 275, 549, 870
BmsI	GCATC	7 cut(s)	245, 631, 797, 1174, 1406, 1453, 1492
BpiI	GAAGAC	1 cut(s)	1528
Bpu10I	CCTNAGC	1 cut(s)	1752
BpuEI	CTTGAG	3 cut(s)	1130, 1233, 1867
BsaI	GGTCTC	1 cut(s)	399
BsaJI	CCNNGG	4 cut(s)	415, 811, 972, 1646
BsaWI	WCCGGW	2 cut(s)	305, 408
Bsc4I	CCNNNNNNNGG	4 cut(s)	691, 862, 1336, 1337
Bse118I	RCCGGY	2 cut(s)	305, 408
Bse1I	ACTGG	2 cut(s)	47, 1015
Bse3DI	GCAATG	2 cut(s)	686, 1227
BseBI	CCWGG	5 cut(s)	36, 255, 275, 549, 870
BseDI	CCNNGG	4 cut(s)	415, 811, 972, 1646
BseGI	GGATG	3 cut(s)	621, 788, 1397
BseLI	CCNNNNNNNGG	4 cut(s)	691, 862, 1336, 1337
BseMI	GCAATG	2 cut(s)	686, 1227
BseMII	CTCAG	5 cut(s)	990, 1040, 1636, 1691, 1766
BseNI	ACTGG	2 cut(s)	47, 1015
BseSI	GKGCMC	1 cut(s)	1331
BseXI	GCAGC	8 cut(s)	718, 812, 1145, 1517, 1549, 1573, 1582, 1595
BseYI	CCCAGC	1 cut(s)	674
BsgI	GTGCAG	3 cut(s)	54, 1602, 1621
BshFI	GGCC	4 cut(s)	373, 456, 1329, 1353
BshNI	GGYRCC	1 cut(s)	411
BshTI	ACCGGT	2 cut(s)	305, 408
BsiHKAI	GWGCWC	3 cut(s)	134, 1308, 1623
BsiHKCI	CYCGRG	1 cut(s)	474
BsiSI	CCGG	2 cut(s)	306, 409
BslFI	GGGAC	1 cut(s)	1001
BslI	CCNNNNNNNGG	4 cut(s)	691, 862, 1336, 1337
BsmAI	GTCTC	3 cut(s)	324, 399, 1416
BsmFI	GGGAC	1 cut(s)	1001
BsmI	GAATGC	1 cut(s)	1399
BsnI	GGCC	4 cut(s)	373, 456, 1329, 1353
Bso31I	GGTCTC	1 cut(s)	399
BsoBI	CYCGRG	1 cut(s)	474
Bsp120I	GGGCCC	1 cut(s)	1327
Bsp1286I	GDGCHC	4 cut(s)	134, 1308, 1331, 1623
Bsp143I	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
BspACI	CCGC	1 cut(s)	603
BspANI	GGCC	4 cut(s)	373, 456, 1329, 1353
BspCNI	CTCAG	5 cut(s)	991, 1039, 1635, 1690, 1765
BspHI	TCATGA	1 cut(s)	895
BspLI	GGNNCC	9 cut(s)	304, 413, 532, 696, 867, 1328, 1329, 1722, 1857
BspPI	GGATC	4 cut(s)	559, 1715, 1728, 1811
BspQI	GCTCTTC	1 cut(s)	1611
BspT107I	GGYRCC	1 cut(s)	411
BspTNI	GGTCTC	1 cut(s)	399
BsrDI	GCAATG	2 cut(s)	686, 1227
BsrFI	RCCGGY	2 cut(s)	305, 408
BsrI	ACTGG	2 cut(s)	47, 1015
BssAI	RCCGGY	2 cut(s)	305, 408
BssECI	CCNNGG	4 cut(s)	415, 811, 972, 1646
BssMI	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
BssT1I	CCWWGG	2 cut(s)	415, 972
Bst2UI	CCWGG	5 cut(s)	36, 255, 275, 549, 870
Bst4CI	ACNGT	6 cut(s)	189, 202, 655, 1072, 1087, 1178
Bst6I	CTCTTC	2 cut(s)	870, 1611
BstC8I	GCNNGC	5 cut(s)	141, 330, 805, 1167, 1600
BstDEI	CTNAG	6 cut(s)	999, 1026, 1319, 1622, 1677, 1752
BstDSI	CCRYGG	1 cut(s)	811
BstF5I	GGATG	3 cut(s)	621, 788, 1397
BstHHI	GCGC	4 cut(s)	674, 1429, 1714, 1782
BstKTI	GATC	5 cut(s)	554, 1408, 1522, 1723, 1819
BstMAI	GTCTC	3 cut(s)	324, 399, 1416
BstMBI	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
BstMWI	GCNNNNNNNGC	4 cut(s)	1559, 1592, 1595, 1786
BstNI	CCWGG	5 cut(s)	36, 255, 275, 549, 870
BstNSI	RCATGY	4 cut(s)	143, 332, 353, 887
BstSCI	CCNGG	5 cut(s)	34, 253, 273, 547, 868
BstSFI	CTRYAG	1 cut(s)	342
BstSLI	GKGCMC	1 cut(s)	1331
BstV1I	GCAGC	8 cut(s)	718, 812, 1145, 1517, 1549, 1573, 1582, 1595
BstV2I	GAAGAC	1 cut(s)	1528
BstX2I	RGATCY	2 cut(s)	551, 1720
BstXI	CCANNNNNNTGG	1 cut(s)	117
BstYI	RGATCY	2 cut(s)	551, 1720
BsuRI	GGCC	4 cut(s)	373, 456, 1329, 1353
BtgI	CCRYGG	1 cut(s)	811
BtsCI	GGATG	3 cut(s)	621, 788, 1397
BtsI	GCAGTG	1 cut(s)	335
BtsIMutI	CAGTG	2 cut(s)	335, 1630
Cac8I	GCNNGC	5 cut(s)	141, 330, 805, 1167, 1600
CaiI	CAGNNNCTG	2 cut(s)	53, 1033
CciI	TCATGA	1 cut(s)	895
CfoI	GCGC	4 cut(s)	674, 1429, 1714, 1782
Cfr10I	RCCGGY	2 cut(s)	305, 408
Cfr13I	GGNCC	6 cut(s)	455, 530, 545, 866, 1327, 1328
CsiI	ACCWGGT	1 cut(s)	34
Csp6I	GTAC	5 cut(s)	185, 1253, 1458, 1514, 1838
CspAI	ACCGGT	2 cut(s)	305, 408
CviAII	CATG	9 cut(s)	140, 270, 329, 350, 746, 884, 896, 1355, 1523
CviQI	GTAC	5 cut(s)	185, 1253, 1458, 1514, 1838
DdeI	CTNAG	6 cut(s)	999, 1026, 1319, 1622, 1677, 1752
DpnI	GATC	5 cut(s)	553, 1407, 1521, 1722, 1818
DpnII	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
DraI	TTTAAA	1 cut(s)	1236
EaeI	YGGCCR	1 cut(s)	1351
Eam1104I	CTCTTC	2 cut(s)	870, 1611
EarI	CTCTTC	2 cut(s)	870, 1611
Ecl136II	GAGCTC	2 cut(s)	132, 1621
Eco130I	CCWWGG	2 cut(s)	415, 972
Eco24I	GRGCYC	3 cut(s)	134, 1331, 1623
Eco31I	GGTCTC	1 cut(s)	399
Eco47I	GGWCC	3 cut(s)	530, 545, 866
Eco53kI	GAGCTC	2 cut(s)	132, 1621
Eco57I	CTGAAG	1 cut(s)	1635
Eco88I	CYCGRG	1 cut(s)	474
EcoICRI	GAGCTC	2 cut(s)	132, 1621
EcoO109I	RGGNCCY	2 cut(s)	866, 1327
EcoRII	CCWGG	5 cut(s)	34, 253, 273, 547, 868
EcoT14I	CCWWGG	2 cut(s)	415, 972
EcoT22I	ATGCAT	3 cut(s)	330, 1399, 1446
EcoT38I	GRGCYC	3 cut(s)	134, 1331, 1623
ErhI	CCWWGG	2 cut(s)	415, 972
FaeI	CATG	9 cut(s)	143, 273, 332, 353, 749, 887, 899, 1358, 1526
FaqI	GGGAC	1 cut(s)	1001
FatI	CATG	9 cut(s)	139, 269, 328, 349, 745, 883, 895, 1354, 1522
FbaI	TGATCA	1 cut(s)	1519
FblI	GTMKAC	1 cut(s)	888
Fnu4HI	GCNGC	8 cut(s)	707, 801, 1134, 1506, 1563, 1584, 1587, 1596
FokI	GGATG	3 cut(s)	628, 775, 1384
FriOI	GRGCYC	3 cut(s)	134, 1331, 1623
Fsp4HI	GCNGC	8 cut(s)	707, 801, 1134, 1506, 1563, 1584, 1587, 1596
FspBI	CTAG	3 cut(s)	60, 402, 1821
FspI	TGCGCA	1 cut(s)	1713
GlaI	GCGC	4 cut(s)	673, 1428, 1713, 1781
GluI	GCNGC	8 cut(s)	707, 801, 1134, 1506, 1563, 1584, 1587, 1596
GsaI	CCCAGC	1 cut(s)	678
HaeIII	GGCC	4 cut(s)	373, 456, 1329, 1353
HapII	CCGG	2 cut(s)	306, 409
HhaI	GCGC	4 cut(s)	674, 1429, 1714, 1782
Hin1II	CATG	9 cut(s)	143, 273, 332, 353, 749, 887, 899, 1358, 1526
Hin6I	GCGC	4 cut(s)	672, 1427, 1712, 1780
HinP1I	GCGC	4 cut(s)	672, 1427, 1712, 1780
HindIII	AAGCTT	3 cut(s)	396, 720, 1843
HinfI	GANTC	7 cut(s)	56, 152, 227, 278, 510, 1156, 1651
HpaII	CCGG	2 cut(s)	306, 409
HphI	GGTGA	4 cut(s)	258, 566, 770, 884
Hpy166II	GTNNAC	5 cut(s)	530, 775, 889, 1020, 1628
Hpy188I	TCNGA	8 cut(s)	55, 223, 838, 1035, 1183, 1392, 1501, 1650
Hpy8I	GTNNAC	5 cut(s)	530, 775, 889, 1020, 1628
HpyAV	CCTTC	6 cut(s)	280, 1317, 1445, 1565, 1661, 1669
HpyCH4III	ACNGT	6 cut(s)	189, 202, 655, 1072, 1087, 1178
HpyCH4IV	ACGT	1 cut(s)	902
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1559, 1592, 1595, 1786
HpyF3I	CTNAG	6 cut(s)	999, 1026, 1319, 1622, 1677, 1752
HpySE526I	ACGT	1 cut(s)	902
Hsp92II	CATG	9 cut(s)	143, 273, 332, 353, 749, 887, 899, 1358, 1526
HspAI	GCGC	4 cut(s)	672, 1427, 1712, 1780
Ksp22I	TGATCA	1 cut(s)	1519
Kzo9I	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
LguI	GCTCTTC	1 cut(s)	1611
LmnI	GCTCC	2 cut(s)	137, 619
Lsp1109I	GCAGC	8 cut(s)	718, 812, 1145, 1517, 1549, 1573, 1582, 1595
LweI	GCATC	7 cut(s)	245, 631, 797, 1174, 1406, 1453, 1492
MabI	ACCWGGT	1 cut(s)	34
MaeI	CTAG	3 cut(s)	60, 402, 1821
MaeII	ACGT	1 cut(s)	902
MaeIII	GTNAC	2 cut(s)	572, 1374
MalI	GATC	5 cut(s)	553, 1407, 1521, 1722, 1818
MboI	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
MboII	GAAGA	5 cut(s)	887, 959, 1370, 1528, 1628
MfeI	CAATTG	1 cut(s)	1188
MflI	RGATCY	2 cut(s)	551, 1720
MhlI	GDGCHC	4 cut(s)	134, 1308, 1331, 1623
MlsI	TGGCCA	1 cut(s)	1353
MluCI	AATT	7 cut(s)	174, 380, 447, 580, 825, 1188, 1218
MluNI	TGGCCA	1 cut(s)	1353
MlyI	GAGTC	3 cut(s)	287, 519, 1150
Mox20I	TGGCCA	1 cut(s)	1353
Mph1103I	ATGCAT	3 cut(s)	330, 1399, 1446
MscI	TGGCCA	1 cut(s)	1353
MseI	TTAA	3 cut(s)	368, 1221, 1235
MslI	CAYNNNNRTG	3 cut(s)	610, 932, 1359
Msp20I	TGGCCA	1 cut(s)	1353
MspA1I	CMGCKG	1 cut(s)	1586
MspI	CCGG	2 cut(s)	306, 409
MspR9I	CCNGG	5 cut(s)	36, 255, 275, 549, 870
MunI	CAATTG	1 cut(s)	1188
Mva1269I	GAATGC	1 cut(s)	1399
MvaI	CCWGG	5 cut(s)	36, 255, 275, 549, 870
MwoI	GCNNNNNNNGC	4 cut(s)	1559, 1592, 1595, 1786
NdeII	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
NlaIII	CATG	9 cut(s)	143, 273, 332, 353, 749, 887, 899, 1358, 1526
NlaIV	GGNNCC	9 cut(s)	304, 413, 532, 696, 867, 1328, 1329, 1722, 1857
NmuCI	GTSAC	1 cut(s)	572
NsbI	TGCGCA	1 cut(s)	1713
NsiI	ATGCAT	3 cut(s)	330, 1399, 1446
NspI	RCATGY	4 cut(s)	143, 332, 353, 887
PaeI	GCATGC	2 cut(s)	143, 332
PaeR7I	CTCGAG	1 cut(s)	474
PagI	TCATGA	1 cut(s)	895
PciI	ACATGT	1 cut(s)	883
PciSI	GCTCTTC	1 cut(s)	1611
PctI	GAATGC	1 cut(s)	1399
PfeI	GAWTC	4 cut(s)	56, 152, 227, 1651
PflMI	CCANNNNNTGG	1 cut(s)	862
PfoI	TCCNGGA	1 cut(s)	547
PinAI	ACCGGT	2 cut(s)	305, 408
PkrI	GCNGC	8 cut(s)	708, 802, 1135, 1507, 1564, 1585, 1588, 1597
PleI	GAGTC	3 cut(s)	286, 518, 1150
PpsI	GAGTC	3 cut(s)	286, 518, 1150
PpuMI	RGGWCCY	1 cut(s)	866
PscI	ACATGT	1 cut(s)	883
Psp124BI	GAGCTC	2 cut(s)	134, 1623
Psp1406I	AACGTT	1 cut(s)	902
Psp5II	RGGWCCY	1 cut(s)	866
Psp6I	CCWGG	5 cut(s)	34, 253, 273, 547, 868
PspFI	CCCAGC	1 cut(s)	674
PspGI	CCWGG	5 cut(s)	34, 253, 273, 547, 868
PspN4I	GGNNCC	9 cut(s)	304, 413, 532, 696, 867, 1328, 1329, 1722, 1857
PspOMI	GGGCCC	1 cut(s)	1327
PspPI	GGNCC	6 cut(s)	455, 530, 545, 866, 1327, 1328
PspPPI	RGGWCCY	1 cut(s)	866
PstNI	CAGNNNCTG	2 cut(s)	53, 1033
PsuI	RGATCY	2 cut(s)	551, 1720
PvuII	CAGCTG	1 cut(s)	1586
RsaI	GTAC	5 cut(s)	186, 1254, 1459, 1515, 1839
RsaNI	GTAC	5 cut(s)	185, 1253, 1458, 1514, 1838
RseI	CAYNNNNRTG	3 cut(s)	610, 932, 1359
SacI	GAGCTC	2 cut(s)	134, 1623
SapI	GCTCTTC	1 cut(s)	1611
SaqAI	TTAA	3 cut(s)	368, 1221, 1235
SatI	GCNGC	8 cut(s)	707, 801, 1134, 1506, 1563, 1584, 1587, 1596
Sau3AI	GATC	5 cut(s)	551, 1405, 1519, 1720, 1816
Sau96I	GGNCC	6 cut(s)	455, 530, 545, 866, 1327, 1328
ScaI	AGTACT	1 cut(s)	186
SchI	GAGTC	3 cut(s)	287, 519, 1150
ScrFI	CCNGG	5 cut(s)	36, 255, 275, 549, 870
SduI	GDGCHC	4 cut(s)	134, 1308, 1331, 1623
SexAI	ACCWGGT	1 cut(s)	34
SfaNI	GCATC	7 cut(s)	245, 631, 797, 1174, 1406, 1453, 1492
SfcI	CTRYAG	1 cut(s)	342
Sfr274I	CTCGAG	1 cut(s)	474
SinI	GGWCC	3 cut(s)	530, 545, 866
SlaI	CTCGAG	1 cut(s)	474
SmiMI	CAYNNNNRTG	3 cut(s)	610, 932, 1359
SmlI	CTYRAG	4 cut(s)	474, 1109, 1248, 1846
SmoI	CTYRAG	4 cut(s)	474, 1109, 1248, 1846
SphI	GCATGC	2 cut(s)	143, 332
Sse9I	AATT	7 cut(s)	174, 380, 447, 580, 825, 1188, 1218
SsiI	CCGC	1 cut(s)	603
SspMI	CTAG	3 cut(s)	60, 402, 1821
SstI	GAGCTC	2 cut(s)	134, 1623
StyD4I	CCNGG	5 cut(s)	34, 253, 273, 547, 868
StyI	CCWWGG	2 cut(s)	415, 972
TaaI	ACNGT	6 cut(s)	189, 202, 655, 1072, 1087, 1178
TaiI	ACGT	1 cut(s)	905
TaqI	TCGA	3 cut(s)	150, 475, 945
TasI	AATT	7 cut(s)	174, 380, 447, 580, 825, 1188, 1218
TatI	WGTACW	2 cut(s)	184, 1513
TfiI	GAWTC	4 cut(s)	56, 152, 227, 1651
Tru1I	TTAA	3 cut(s)	368, 1221, 1235
Tru9I	TTAA	3 cut(s)	368, 1221, 1235
TscAI	CASTG	2 cut(s)	342, 1630
TseFI	GTSAC	1 cut(s)	572
TseI	GCWGC	8 cut(s)	706, 800, 1133, 1505, 1562, 1583, 1586, 1595
Tsp45I	GTSAC	1 cut(s)	572
TspDTI	ATGAA	7 cut(s)	120, 912, 1227, 1304, 1371, 1528, 1584
TspGWI	ACGGA	2 cut(s)	800, 1393
TspRI	CASTG	2 cut(s)	342, 1630
Van91I	CCANNNNNTGG	1 cut(s)	862
VpaK11BI	GGWCC	3 cut(s)	530, 545, 866
XbaI	TCTAGA	2 cut(s)	59, 401
XceI	RCATGY	4 cut(s)	143, 332, 353, 887
XcmI	CCANNNNNNNNNTGG	1 cut(s)	752
XhoI	CTCGAG	1 cut(s)	474
XmiI	GTMKAC	1 cut(s)	888
XspI	CTAG	3 cut(s)	60, 402, 1821
ZrmI	AGTACT	1 cut(s)	186
Zsp2I	ATGCAT	3 cut(s)	330, 1399, 1446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.