MD10G1003100.v1.1

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006172
Physical Location & Seq
Forward (+)
638242 .. 640333
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1003100.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGAGGATCAGAATCAGATAGACGGAAACAAAGAAAACAAAAAACGTGCTTCATCAGGCTCTCTTTTGCAGCAGAATCAATGGTCACTGCTTGAATCTGACCTCCCAACAGATCCTATGGACGTAGACAGAGAAACCAAAAAACGTGCTTCATCGGACTCCTCCTTACAGTGGAGCCAATGGCAACTGCTCGATTCAATTCTCCCAACTGGCGGTTTTGCTCATTCTTTTGGTCTTGAGGCTGCAATCCAAGCTCGCATAGTCTCCGGTCCTGAAGATCTCCAAACTTTCATAATACATCTGTTGGAGAACGCTGGAAGCTTACTGCTTCCTTTTGTGTATTCTGCAACAGTATCACCTGATTCGGAGACCAGGCGTAGACTCGACAAAATCTTGGATGCAATGCTGACTAATGAAGTGAGTAGAAAGGCATCAATTGCACAAGGGTCGGCACTGATGAGGGTGGCTGCATCTGTGTATTCAGAAATACCGTATCTTAAATCTATGAGGGAAGCTTGTTTGAGTACTGGTGTTGTTTCTTTTCACCATGCTCCTCTGTTTGGGGTTATATGCGGACTGCTTGGGTTGGATAGCACTACTTCTCAAAGGGCTTACATGTTCATTACAATGAGAGACGTTATTTCTGCTGCGACGAGGCTCAATTTGGTAGGACCCCTTGGTGCGGCAGTACTGCAGCATCAGATTGGTCCAGTTGCCGAAGCTATACTGAATCAATGGAAGGACCGTCCTGTTGAGGAAGCATGCCAAACTGTTGCTTTGCTTGATATCGTGCAGGGATGTCATGGCTACTTGTTTTCTAGATTGTTCTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0051336 GO:0051345 GO:0065007 GO:0065009 GO:1905181 GO:1905182
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UreF

Protein Analysis

279

Amino Acids

30.48

Weight (kDa)

5.53

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UreF PF01730 88 - 234 1.5e-24 UreF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 126, 379
AciI CCGC 3 cut(s) 213, 573, 683
AclWI GGATC 2 cut(s) 15, 107
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 2 cut(s) 526, 690
AfiI CCNNNNNNNGG 4 cut(s) 171, 212, 560, 682
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 2 cut(s) 95, 198
AjnI CCWGG 1 cut(s) 371
AluBI AGCT 4 cut(s) 254, 321, 515, 722
AluI AGCT 4 cut(s) 254, 321, 515, 722
Alw26I GTCTC 3 cut(s) 268, 362, 627
AlwI GGATC 2 cut(s) 15, 107
ApeKI GCWGC 5 cut(s) 70, 242, 467, 647, 694
AspS9I GGNCC 4 cut(s) 269, 671, 707, 742
AsuHPI GGTGA 2 cut(s) 348, 536
AvaII GGWCC 4 cut(s) 269, 671, 707, 742
BbvI GCAGC 5 cut(s) 82, 229, 454, 634, 706
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BciT130I CCWGG 1 cut(s) 373
BcoDI GTCTC 3 cut(s) 268, 362, 627
BfaI CTAG 1 cut(s) 819
BfmI CTRYAG 1 cut(s) 692
BglII AGATCT 1 cut(s) 277
BisI GCNGC 6 cut(s) 71, 243, 468, 648, 684, 695
BlsI GCNGC 6 cut(s) 72, 244, 469, 649, 685, 696
BmcAI AGTACT 2 cut(s) 526, 690
Bme1390I CCNGG 1 cut(s) 373
Bme18I GGWCC 4 cut(s) 269, 671, 707, 742
BmgT120I GGNCC 4 cut(s) 269, 671, 707, 742
BmiI GGNNCC 2 cut(s) 176, 673
BmrFI CCNGG 1 cut(s) 373
BmsI GCATC 4 cut(s) 388, 440, 479, 706
BpuEI CTTGAG 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 676
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 212, 560, 682
Bse1I ACTGG 3 cut(s) 214, 532, 710
Bse3DI GCAATG 1 cut(s) 408
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 676
BseGI GGATG 2 cut(s) 403, 803
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 4 cut(s) 171, 212, 560, 682
BseMI GCAATG 1 cut(s) 408
BseNI ACTGG 3 cut(s) 214, 532, 710
BseRI GAGGAG 2 cut(s) 151, 543
BseXI GCAGC 5 cut(s) 82, 229, 454, 634, 706
BsgI GTGCAG 1 cut(s) 812
BsiSI CCGG 1 cut(s) 267
BslI CCNNNNNNNGG 4 cut(s) 171, 212, 560, 682
BsmAI GTCTC 3 cut(s) 268, 362, 627
BsmBI CGTCTC 1 cut(s) 627
Bso31I GGTCTC 1 cut(s) 362
Bsp143I GATC 3 cut(s) 7, 112, 277
BspACI CCGC 3 cut(s) 213, 573, 683
BspLI GGNNCC 2 cut(s) 176, 673
BspMAI CTGCAG 1 cut(s) 696
BspPI GGATC 2 cut(s) 15, 107
BspTNI GGTCTC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 3 cut(s) 214, 532, 710
BssECI CCNNGG 1 cut(s) 676
BssMI GATC 3 cut(s) 7, 112, 277
BssT1I CCWWGG 1 cut(s) 676
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 5 cut(s) 171, 352, 492, 746, 772
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 256, 763
BstF5I GGATG 2 cut(s) 403, 803
BstKTI GATC 3 cut(s) 10, 115, 280
BstMAI GTCTC 3 cut(s) 268, 362, 627
BstMBI GATC 3 cut(s) 7, 112, 277
BstMWI GCNNNNNNNGC 2 cut(s) 251, 437
BstNI CCWGG 1 cut(s) 373
BstNSI RCATGY 2 cut(s) 619, 765
BstSCI CCNGG 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 692
BstV1I GCAGC 5 cut(s) 82, 229, 454, 634, 706
BstX2I RGATCY 2 cut(s) 112, 277
BstYI RGATCY 2 cut(s) 112, 277
BtsCI GGATG 2 cut(s) 403, 803
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 3 cut(s) 86, 176, 452
Cac8I GCNNGC 2 cut(s) 256, 763
Cfr13I GGNCC 4 cut(s) 269, 671, 707, 742
Csp6I GTAC 2 cut(s) 525, 689
CviAII CATG 4 cut(s) 548, 616, 762, 803
CviQI GTAC 2 cut(s) 525, 689
DpnI GATC 3 cut(s) 9, 114, 279
DpnII GATC 3 cut(s) 7, 112, 277
Eco130I CCWWGG 1 cut(s) 676
Eco31I GGTCTC 1 cut(s) 362
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 4 cut(s) 269, 671, 707, 742
Eco57I CTGAAG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 371
EcoRV GATATC 1 cut(s) 787
EcoT14I CCWWGG 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 676
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 4 cut(s) 551, 619, 765, 806
FatI CATG 4 cut(s) 547, 615, 761, 802
FblI GTMKAC 2 cut(s) 126, 379
Fnu4HI GCNGC 6 cut(s) 71, 243, 468, 648, 684, 695
FokI GGATG 2 cut(s) 410, 810
Fsp4HI GCNGC 6 cut(s) 71, 243, 468, 648, 684, 695
FspBI CTAG 1 cut(s) 819
GluI GCNGC 6 cut(s) 71, 243, 468, 648, 684, 695
HapII CCGG 1 cut(s) 267
Hin1II CATG 4 cut(s) 551, 619, 765, 806
HindIII AAGCTT 2 cut(s) 319, 513
HinfI GANTC 8 cut(s) 13, 76, 95, 158, 194, 362, 381, 730
HpaII CCGG 1 cut(s) 267
HphI GGTGA 2 cut(s) 348, 536
Hpy166II GTNNAC 2 cut(s) 127, 380
Hpy188I TCNGA 7 cut(s) 12, 18, 100, 157, 367, 484, 702
Hpy188III TCNNGA 4 cut(s) 236, 272, 819, 834
Hpy8I GTNNAC 2 cut(s) 127, 380
Hpy99I CGWCG 1 cut(s) 655
HpyAV CCTTC 1 cut(s) 733
HpyCH4III ACNGT 5 cut(s) 171, 352, 492, 746, 772
HpyCH4IV ACGT 4 cut(s) 46, 123, 145, 636
HpyCH4V TGCA 8 cut(s) 70, 245, 347, 401, 440, 470, 694, 793
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 437
HpySE526I ACGT 4 cut(s) 46, 123, 145, 636
Hsp92II CATG 4 cut(s) 551, 619, 765, 806
Kzo9I GATC 3 cut(s) 7, 112, 277
LmnI GCTCC 2 cut(s) 174, 556
Lsp1109I GCAGC 5 cut(s) 82, 229, 454, 634, 706
LweI GCATC 4 cut(s) 388, 440, 479, 706
MaeI CTAG 1 cut(s) 819
MaeII ACGT 4 cut(s) 46, 123, 145, 636
MaeIII GTNAC 1 cut(s) 84
MalI GATC 3 cut(s) 9, 114, 279
MboI GATC 3 cut(s) 7, 112, 277
MboII GAAGA 1 cut(s) 287
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 2 cut(s) 112, 277
MluCI AATT 3 cut(s) 198, 435, 661
MlyI GAGTC 2 cut(s) 152, 375
MmeI TCCRAC 2 cut(s) 285, 567
MnlI CCTC 8 cut(s) 113, 172, 232, 453, 501, 564, 648, 748
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 1 cut(s) 626
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 373
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 373
MwoI GCNNNNNNNGC 2 cut(s) 251, 437
NdeII GATC 3 cut(s) 7, 112, 277
NlaIII CATG 4 cut(s) 551, 619, 765, 806
NlaIV GGNNCC 2 cut(s) 176, 673
NmuCI GTSAC 1 cut(s) 84
NspI RCATGY 2 cut(s) 619, 765
PaeI GCATGC 1 cut(s) 765
PciI ACATGT 1 cut(s) 615
PfeI GAWTC 6 cut(s) 13, 76, 95, 194, 362, 730
PkrI GCNGC 6 cut(s) 72, 244, 469, 649, 685, 696
PleI GAGTC 2 cut(s) 152, 375
PpsI GAGTC 2 cut(s) 152, 375
PpuMI RGGWCCY 1 cut(s) 671
PscI ACATGT 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 371
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 176, 673
PspPI GGNCC 4 cut(s) 269, 671, 707, 742
PspPPI RGGWCCY 1 cut(s) 671
PstI CTGCAG 1 cut(s) 696
PsuI RGATCY 2 cut(s) 112, 277
RsaI GTAC 2 cut(s) 526, 690
RsaNI GTAC 2 cut(s) 525, 689
RseI CAYNNNNRTG 1 cut(s) 626
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 6 cut(s) 71, 243, 468, 648, 684, 695
Sau3AI GATC 3 cut(s) 7, 112, 277
Sau96I GGNCC 4 cut(s) 269, 671, 707, 742
ScaI AGTACT 2 cut(s) 526, 690
SchI GAGTC 2 cut(s) 152, 375
ScrFI CCNGG 1 cut(s) 373
SfaNI GCATC 4 cut(s) 388, 440, 479, 706
SfcI CTRYAG 1 cut(s) 692
SinI GGWCC 4 cut(s) 269, 671, 707, 742
SmiMI CAYNNNNRTG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SphI GCATGC 1 cut(s) 765
Sse9I AATT 3 cut(s) 198, 435, 661
SsiI CCGC 3 cut(s) 213, 573, 683
SspMI CTAG 1 cut(s) 819
StyD4I CCNGG 1 cut(s) 371
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 5 cut(s) 171, 352, 492, 746, 772
TaiI ACGT 4 cut(s) 49, 126, 148, 639
TaqI TCGA 2 cut(s) 192, 384
TasI AATT 3 cut(s) 198, 435, 661
TatI WGTACW 2 cut(s) 524, 688
TauI GCSGC 1 cut(s) 686
TfiI GAWTC 6 cut(s) 13, 76, 95, 194, 362, 730
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 3 cut(s) 93, 176, 459
TseFI GTSAC 1 cut(s) 84
TseI GCWGC 5 cut(s) 70, 242, 467, 647, 694
Tsp45I GTSAC 1 cut(s) 84
TspDTI ATGAA 5 cut(s) 42, 141, 280, 429, 610
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 3 cut(s) 93, 176, 459
VpaK11BI GGWCC 4 cut(s) 269, 671, 707, 742
XbaI TCTAGA 1 cut(s) 818
XceI RCATGY 2 cut(s) 619, 765
XmiI GTMKAC 2 cut(s) 126, 379
XspI CTAG 1 cut(s) 819
ZrmI AGTACT 2 cut(s) 526, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.