RchiOBHm_Chr6g0250481

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006172
Physical Location & Seq
Forward (+)
5991396 .. 5994536
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22453

Sequence Viewer

Length: 720 bp
ATGGATATACACAAAGAAAGCAAGGAGCATGCTTCGTCTGAATCCCTTTTACAATGGGGCCAATGGCAACTGCTTGATTCTATCCTCCCAACCGGTGGTTTTGCACATTCCTTTGGTCTTGAGGCTGCAATCCAAGCTCGGATAGTCTCTGGTCCTGAAGATCTTCAGACTTTTGTAATTCATGTGTTAGAGAATACGGCAAGCTTACTCCTTCCTTTTGTGTATTCTACAACAATATCGCCTGATTTGGAGAGTTTGCGTAGACATGACAAGATATTGGACGCAATGCTAACTAATGAAGTGGGTAGAAAGGCATCAATTGCACAAGGGTCTGCGCTGATGCGAGTGGCTGCAGCTGTTTATTCGGAAATACCATCTCTTAAATCTATGAGGGAAACTTCTTTGAGTAATGGAGTTGTTTCTTTTCATCATGCTCCTATATTTGGGCTTATATGTGGATTGCTTGGGTTCGATAGCAGTACTTCTCAAAGAGCTTTCATGTTTATCACAATGAGAGATATTATTTCTGCTGCAACAAGGATGAATTTGGTTGGACCCCTAGGCGCGGCTGTTCTGCAACATCATATTGCTCCTATCTCTGAAGCTATACTGAATCAATGGAAGGACCGTCCAGTTGAGGAAGCATGCCAAACTGTTGCTTTGCTTGATATCGTGCAGGGTTGTCATAGCTACCTGTTTTCTAGACTATTCTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0051336 GO:0051345 GO:0065007 GO:0065009 GO:1905181 GO:1905182
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UreF

Protein Analysis

239

Amino Acids

26.16

Weight (kDa)

5.9

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UreF PF01730 49 - 196 5.8e-23 UreF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AccI GTMKAC 1 cut(s) 262
AccII CGCG 1 cut(s) 566
AciI CCGC 1 cut(s) 566
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 3 cut(s) 149, 177, 621
AfaI GTAC 1 cut(s) 481
AfiI CCNNNNNNNGG 3 cut(s) 95, 443, 565
AgeI ACCGGT 1 cut(s) 92
AluBI AGCT 6 cut(s) 137, 204, 356, 494, 605, 690
AluI AGCT 6 cut(s) 137, 204, 356, 494, 605, 690
Alw26I GTCTC 1 cut(s) 151
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 4 cut(s) 125, 350, 353, 530
ApoI RAATTY 1 cut(s) 544
AsiGI ACCGGT 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 162
AspA2I CCTAGG 1 cut(s) 559
AspLEI GCGC 2 cut(s) 337, 566
AspS9I GGNCC 4 cut(s) 58, 152, 554, 625
AvaII GGWCC 3 cut(s) 152, 554, 625
AvrII CCTAGG 1 cut(s) 559
BbvI GCAGC 4 cut(s) 112, 337, 365, 517
BccI CCATC 1 cut(s) 382
BceAI ACGGC 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 2 cut(s) 560, 702
BfmI CTRYAG 1 cut(s) 351
BglII AGATCT 1 cut(s) 160
BisI GCNGC 5 cut(s) 126, 351, 354, 531, 567
BlnI CCTAGG 1 cut(s) 559
BlsI GCNGC 5 cut(s) 127, 352, 355, 532, 568
BmcAI AGTACT 1 cut(s) 481
Bme18I GGWCC 3 cut(s) 152, 554, 625
BmgT120I GGNCC 4 cut(s) 58, 152, 554, 625
BmiI GGNNCC 2 cut(s) 59, 556
BmsI GCATC 2 cut(s) 323, 330
BpuEI CTTGAG 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 559
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 443, 565
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 632
Bse3DI GCAATG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 559
BseGI GGATG 1 cut(s) 546
BseLI CCNNNNNNNGG 3 cut(s) 95, 443, 565
BseMI GCAATG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 632
BseXI GCAGC 4 cut(s) 112, 337, 365, 517
BsgI GTGCAG 1 cut(s) 695
Bsh1236I CGCG 1 cut(s) 566
BshFI GGCC 1 cut(s) 60
BshTI ACCGGT 1 cut(s) 92
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 95, 443, 565
BsmAI GTCTC 1 cut(s) 151
BsnI GGCC 1 cut(s) 60
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 1 cut(s) 566
BspANI GGCC 1 cut(s) 60
BspFNI CGCG 1 cut(s) 566
BspLI GGNNCC 2 cut(s) 59, 556
BspMAI CTGCAG 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 632
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 559
BssMI GATC 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 629, 655
BstAPI GCANNNNNTGC 1 cut(s) 320
BstC8I GCNNGC 3 cut(s) 30, 202, 646
BstF5I GGATG 1 cut(s) 546
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 2 cut(s) 337, 566
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 1 cut(s) 160
BstMWI GCNNNNNNNGC 2 cut(s) 134, 320
BstNSI RCATGY 2 cut(s) 32, 648
BstSFI CTRYAG 1 cut(s) 351
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 4 cut(s) 112, 337, 365, 517
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 546
Cac8I GCNNGC 3 cut(s) 30, 202, 646
CfoI GCGC 2 cut(s) 337, 566
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 58, 152, 554, 625
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 480
CspAI ACCGGT 1 cut(s) 92
CviAII CATG 6 cut(s) 29, 182, 266, 431, 499, 645
CviQI GTAC 1 cut(s) 480
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 559
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 3 cut(s) 152, 554, 625
Eco57I CTGAAG 3 cut(s) 149, 177, 621
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 559
FaeI CATG 6 cut(s) 32, 185, 269, 434, 502, 648
FatI CATG 6 cut(s) 28, 181, 265, 430, 498, 644
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 5 cut(s) 126, 351, 354, 531, 567
FokI GGATG 1 cut(s) 553
Fsp4HI GCNGC 5 cut(s) 126, 351, 354, 531, 567
FspBI CTAG 2 cut(s) 560, 702
GlaI GCGC 2 cut(s) 336, 565
GluI GCNGC 5 cut(s) 126, 351, 354, 531, 567
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 337, 566
Hin1II CATG 6 cut(s) 32, 185, 269, 434, 502, 648
Hin6I GCGC 2 cut(s) 335, 564
HinP1I GCGC 2 cut(s) 335, 564
HindIII AAGCTT 1 cut(s) 202
HinfI GANTC 3 cut(s) 41, 77, 613
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 5 cut(s) 40, 141, 168, 367, 601
Hpy188III TCNNGA 4 cut(s) 119, 155, 702, 717
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 221, 616
HpyCH4III ACNGT 2 cut(s) 629, 655
HpyCH4V TGCA 7 cut(s) 104, 128, 323, 353, 533, 577, 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 320
Hsp92II CATG 6 cut(s) 32, 185, 269, 434, 502, 648
HspAI GCGC 2 cut(s) 335, 564
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 3 cut(s) 25, 439, 595
LpnPI CCDG 7 cut(s) 106, 135, 168, 255, 645, 662, 707
Lsp1109I GCAGC 4 cut(s) 112, 337, 365, 517
LweI GCATC 2 cut(s) 323, 330
MaeI CTAG 2 cut(s) 560, 702
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 2 cut(s) 155, 170
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 1 cut(s) 160
MluCI AATT 3 cut(s) 177, 318, 544
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 4 cut(s) 95, 115, 384, 631
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 1 cut(s) 93
MunI CAATTG 1 cut(s) 318
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 2 cut(s) 134, 320
NdeII GATC 1 cut(s) 160
NlaIII CATG 6 cut(s) 32, 185, 269, 434, 502, 648
NlaIV GGNNCC 2 cut(s) 59, 556
NspI RCATGY 2 cut(s) 32, 648
PaeI GCATGC 2 cut(s) 32, 648
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 3 cut(s) 41, 77, 613
PflMI CCANNNNNTGG 1 cut(s) 95
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 5 cut(s) 127, 352, 355, 532, 568
PspN4I GGNNCC 2 cut(s) 59, 556
PspPI GGNCC 4 cut(s) 58, 152, 554, 625
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 1 cut(s) 481
RsaNI GTAC 1 cut(s) 480
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 5 cut(s) 126, 351, 354, 531, 567
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 4 cut(s) 58, 152, 554, 625
ScaI AGTACT 1 cut(s) 481
SetI ASST 7 cut(s) 139, 206, 358, 496, 607, 692, 696
SfaNI GCATC 2 cut(s) 323, 330
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 3 cut(s) 152, 554, 625
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SphI GCATGC 2 cut(s) 32, 648
Sse9I AATT 3 cut(s) 177, 318, 544
SsiI CCGC 1 cut(s) 566
SspMI CTAG 2 cut(s) 560, 702
StyI CCWWGG 1 cut(s) 559
TaaI ACNGT 2 cut(s) 629, 655
TaqI TCGA 1 cut(s) 471
TasI AATT 3 cut(s) 177, 318, 544
TatI WGTACW 1 cut(s) 479
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 3 cut(s) 41, 77, 613
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 4 cut(s) 125, 350, 353, 530
TspDTI ATGAA 5 cut(s) 170, 312, 416, 487, 557
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 3 cut(s) 152, 554, 625
XapI RAATTY 1 cut(s) 544
XbaI TCTAGA 1 cut(s) 701
XceI RCATGY 2 cut(s) 32, 648
XmaJI CCTAGG 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 2 cut(s) 560, 702
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.