Rorug05G0497700

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006172
Physical Location & Seq
Reverse (-)
67941649 .. 67943716
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0497700.1

Sequence Viewer

Length: 1482 bp
ATGACAAGTTGTCAAGTTCAAGGAGCTTCTAGTGAGAGTATAACGATGGACAGATTCAGTGATCTCCCAGATGAAATTTCTCATCATATTATTTCGTTCCTCTCTGACTCTTTAAGGAGCCTCATTCAAGTGGGAGCTGTGTCCAAAAGATGTAGGAAACTTTATCTGTCAGTTCCATCAATAATTATTGATGCAAGTAGCGTTGCAGATTGCACCAAGTTGCAAGTAAGATTGTTGAACTCTTGGGATAGGTACTTGTTTCACCGTGGTAATAATAAGATTGAGCGCCTTAGTATTTACTTGGTTTTCAGAACCTCTTGGGAGGCAAAGATAAGTGATGAGTATTTCCGAGTCACCACATGGATCCAAAATGCAGTGAGGTGTAATGTTGAAGTACTTCACCTTGATCTGAATCAATATGACCTCAGGATGACAGTTGTGTTGCCGTCTTGCATCTTCCTTTGTCGTTCTTTGAGGACTCTATCTGTGAATTTGTATATGGGCAAGATTCAGTCACCCAACTTGTCTTTTTCCTGTAATCTCCACTACTTGAAGTTGAAGTATAATTATGAGCTCTGGGATCTTAGCATTTCAGCTGAAAAACTTGAAGATATATGTATACACTGGCAATTTGATTCGAGACGTGATTCAAATTTTCATCTTTTACCTCGCATCAGATCATTTAATCTCTTTGCTCCAAAGCTTAAAAATTTCAAATGGAGTGGGAATTTTCTGAACCATCATCATCTTGGGAACTTGATGTGTTTAGAAGAAGCTGACATATTTCTGATGCCCGATTTAGATGATTCTAGCAACCTATTTGAGGTTCTTTGCAGCATATGTATGGTTAAAGTTCTAACCCTAAATGAAGAAACTACAAAGGCTTTATGCAGGGGAGGTTCCATGCCGGTACCATTAGATAGTATTCATTACTTGAGTTTGCATGTTACAAGTCTGAATGCTGACTTGGTCGCGGCAATGGTGTCTATTTTTAGAGGAATTACTTCTTTGAATACTTTGTGCATAATGTCTGACCTATCCAAGTCATCTGTATCTGGAACTGAAGATGACAGTGATATTGAGGAAGTTGTTGAGGATAATGATACGGAGGAAGAGGATAATGATACAGAGGAAGAGAATAATGAAAACGAGGAAGAGGACAATGACAATTATACCGAGGAAGAAGATAATGAAACTGAGGAAAATGCAACTGAGAAAGAGAATAATGAATCCAGGTTCAATGTGGGATACTGGAAATCCCAAAACCTAACCTTTATTTATCAGCTCAAGGAAGTGAGAATAGACCTTCTAGATGGTAATAATGAATTGGAGTTCGCAAGGTATATTCTGGAGAATGCTCCGAGTTTGGAGAAAATGGTCATAATTCAATCACCCCAGCATTCGGATGTTGTGGAGATGGTAACCAAAAGCAAGATGATTTCCAATGCGACAGTTGTTTTACAAGAAAGGCAGAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0051336 GO:0051345 GO:0065007 GO:0065009 GO:1905181 GO:1905182
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UreF

Protein Analysis

493

Amino Acids

56.71

Weight (kDa)

4.74

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 910
AccB1I GGYRCC 1 cut(s) 910
AccI GTMKAC 1 cut(s) 619
AccII CGCG 1 cut(s) 974
AciI CCGC 1 cut(s) 974
AclWI GGATC 3 cut(s) 358, 371, 588
AcsI RAATTY 5 cut(s) 75, 490, 652, 709, 727
AcuI CTGAAG 1 cut(s) 1083
AfaI GTAC 3 cut(s) 254, 396, 912
AfiI CCNNNNNNNGG 2 cut(s) 823, 1402
AhdI GACNNNNNGTC 1 cut(s) 9
AjiI CACGTC 1 cut(s) 644
AjnI CCWGG 1 cut(s) 1232
AjuI GAANNNNNNNTTGG 2 cut(s) 950, 982
AluBI AGCT 7 cut(s) 26, 137, 574, 596, 703, 776, 1285
AluI AGCT 7 cut(s) 26, 137, 574, 596, 703, 776, 1285
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 1 cut(s) 634
AlwI GGATC 3 cut(s) 358, 371, 588
ApeKI GCWGC 1 cut(s) 834
ApoI RAATTY 5 cut(s) 75, 490, 652, 709, 727
Asp700I GAANNNNTTC 2 cut(s) 396, 1003
Asp718I GGTACC 1 cut(s) 910
AspLEI GCGC 1 cut(s) 288
AsuHPI GGTGA 5 cut(s) 254, 346, 392, 507, 1383
AxyI CCTNAGG 1 cut(s) 425
BamHI GGATCC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 910
BanII GRGCYC 1 cut(s) 576
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 846
BccI CCATC 5 cut(s) 40, 184, 747, 1307, 1411
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 1234
BciVI GTATCC 1 cut(s) 1241
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 3 cut(s) 30, 810, 1310
BfoI RGCGCY 1 cut(s) 289
BfuI GTATCC 1 cut(s) 1241
BisI GCNGC 2 cut(s) 835, 975
BlsI GCNGC 2 cut(s) 836, 976
BmcAI AGTACT 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 1234
BmeRI GACNNNNNGTC 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 644
BmiI GGNNCC 4 cut(s) 119, 365, 901, 912
BmrFI CCNGG 1 cut(s) 1234
BmsI GCATC 4 cut(s) 181, 462, 681, 780
BpmI CTGGAG 1 cut(s) 1370
BpuEI CTTGAG 2 cut(s) 955, 1271
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 265, 1176
Bsc4I CCNNNNNNNGG 2 cut(s) 823, 1402
Bse118I RCCGGY 1 cut(s) 907
Bse1I ACTGG 2 cut(s) 629, 1256
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 984
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 1234
BseDI CCNNGG 2 cut(s) 265, 1176
BseGI GGATG 2 cut(s) 435, 1411
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 823, 1402
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 3 cut(s) 439, 1188, 1203
BseNI ACTGG 2 cut(s) 629, 1256
BseXI GCAGC 1 cut(s) 846
BseYI CCCAGC 1 cut(s) 1395
Bsh1236I CGCG 1 cut(s) 974
BshNI GGYRCC 1 cut(s) 910
BsiHKAI GWGCWC 1 cut(s) 576
BsiSI CCGG 1 cut(s) 908
BslI CCNNNNNNNGG 2 cut(s) 823, 1402
BsmAI GTCTC 1 cut(s) 634
BsmBI CGTCTC 1 cut(s) 634
BsmI GAATGC 3 cut(s) 964, 1360, 1399
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 5 cut(s) 61, 363, 406, 580, 677
BspACI CCGC 1 cut(s) 974
BspCNI CTCAG 3 cut(s) 438, 1189, 1204
BspFNI CGCG 1 cut(s) 974
BspLI GGNNCC 4 cut(s) 119, 365, 901, 912
BspPI GGATC 3 cut(s) 358, 371, 588
BspT107I GGYRCC 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 984
BsrFI RCCGGY 1 cut(s) 907
BsrI ACTGG 2 cut(s) 629, 1256
BssAI RCCGGY 1 cut(s) 907
BssECI CCNNGG 2 cut(s) 265, 1176
BssMI GATC 5 cut(s) 61, 363, 406, 580, 677
BssNAI GTATAC 1 cut(s) 620
Bst1107I GTATAC 1 cut(s) 620
Bst2UI CCWGG 1 cut(s) 1234
Bst4CI ACNGT 4 cut(s) 266, 436, 1073, 1453
Bst6I CTCTTC 3 cut(s) 1107, 1128, 1149
BstDEI CTNAG 5 cut(s) 290, 425, 584, 1197, 1212
BstDSI CCRYGG 1 cut(s) 265
BstEII GGTNACC 1 cut(s) 1420
BstENI CCTNNNNNAGG 1 cut(s) 821
BstF5I GGATG 2 cut(s) 435, 1411
BstFNI CGCG 1 cut(s) 974
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 5 cut(s) 64, 366, 409, 583, 680
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 5 cut(s) 61, 363, 406, 580, 677
BstNI CCWGG 1 cut(s) 1234
BstNSI RCATGY 1 cut(s) 947
BstPI GGTNACC 1 cut(s) 1420
BstSCI CCNGG 1 cut(s) 1232
BstUI CGCG 1 cut(s) 974
BstV1I GCAGC 1 cut(s) 846
BstX2I RGATCY 2 cut(s) 363, 580
BstYI RGATCY 2 cut(s) 363, 580
BstZ17I GTATAC 1 cut(s) 620
Bsu36I CCTNAGG 1 cut(s) 425
BsuI GTATCC 1 cut(s) 1241
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 644
BtsCI GGATG 2 cut(s) 435, 1411
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 4 cut(s) 64, 381, 622, 1078
CfoI GCGC 1 cut(s) 288
Cfr10I RCCGGY 1 cut(s) 907
Csp6I GTAC 3 cut(s) 253, 395, 911
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviAII CATG 3 cut(s) 360, 904, 944
CviJI RGCY 9 cut(s) 26, 120, 137, 574, 596, 703, 776, 884, 1285
CviKI_1 RGCY 9 cut(s) 26, 120, 137, 574, 596, 703, 776, 884, 1285
CviQI GTAC 3 cut(s) 253, 395, 911
DdeI CTNAG 5 cut(s) 290, 425, 584, 1197, 1212
DpnI GATC 5 cut(s) 63, 365, 408, 582, 679
DpnII GATC 5 cut(s) 61, 363, 406, 580, 677
DriI GACNNNNNGTC 1 cut(s) 9
Eam1104I CTCTTC 3 cut(s) 1107, 1128, 1149
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 3 cut(s) 1107, 1128, 1149
Ecl136II GAGCTC 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 576
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 1083
Eco81I CCTNAGG 1 cut(s) 425
Eco91I GGTNACC 1 cut(s) 1420
EcoICRI GAGCTC 1 cut(s) 574
EcoNI CCTNNNNNAGG 1 cut(s) 821
EcoO65I GGTNACC 1 cut(s) 1420
EcoRII CCWGG 1 cut(s) 1232
EcoT38I GRGCYC 1 cut(s) 576
Esp3I CGTCTC 1 cut(s) 634
FaeI CATG 3 cut(s) 363, 907, 947
FatI CATG 3 cut(s) 359, 903, 943
FauNDI CATATG 1 cut(s) 839
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 2 cut(s) 835, 975
FokI GGATG 2 cut(s) 442, 1418
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 2 cut(s) 835, 975
FspBI CTAG 3 cut(s) 30, 810, 1310
GlaI GCGC 1 cut(s) 287
GluI GCNGC 2 cut(s) 835, 975
GsaI CCCAGC 1 cut(s) 1399
GsuI CTGGAG 1 cut(s) 1370
HaeII RGCGCY 1 cut(s) 289
HapII CCGG 1 cut(s) 908
HhaI GCGC 1 cut(s) 288
Hin1II CATG 3 cut(s) 363, 907, 947
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HindIII AAGCTT 1 cut(s) 701
HpaII CCGG 1 cut(s) 908
HphI GGTGA 5 cut(s) 254, 346, 392, 507, 1383
Hpy166II GTNNAC 1 cut(s) 620
Hpy188III TCNNGA 5 cut(s) 427, 639, 1056, 1310, 1349
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 1 cut(s) 1316
HpyCH4III ACNGT 4 cut(s) 266, 436, 1073, 1453
HpyCH4IV ACGT 1 cut(s) 643
HpyF3I CTNAG 5 cut(s) 290, 425, 584, 1197, 1212
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 3 cut(s) 363, 907, 947
HspAI GCGC 1 cut(s) 286
KpnI GGTACC 1 cut(s) 914
Kzo9I GATC 5 cut(s) 61, 363, 406, 580, 677
LmnI GCTCC 5 cut(s) 23, 117, 134, 700, 1363
Lsp1109I GCAGC 1 cut(s) 846
LweI GCATC 4 cut(s) 181, 462, 681, 780
MaeI CTAG 3 cut(s) 30, 810, 1310
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 4 cut(s) 352, 513, 946, 1420
MalI GATC 5 cut(s) 63, 365, 408, 582, 679
MboI GATC 5 cut(s) 61, 363, 406, 580, 677
MflI RGATCY 2 cut(s) 363, 580
MhlI GDGCHC 1 cut(s) 576
MlyI GAGTC 3 cut(s) 101, 360, 472
MroXI GAANNNNTTC 2 cut(s) 396, 1003
MseI TTAA 4 cut(s) 113, 684, 705, 849
MslI CAYNNNNRTG 3 cut(s) 128, 842, 1404
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 1 cut(s) 1234
Mva1269I GAATGC 3 cut(s) 964, 1360, 1399
MvaI CCWGG 1 cut(s) 1234
MvnI CGCG 1 cut(s) 974
NdeI CATATG 1 cut(s) 839
NdeII GATC 5 cut(s) 61, 363, 406, 580, 677
NlaIII CATG 3 cut(s) 363, 907, 947
NlaIV GGNNCC 4 cut(s) 119, 365, 901, 912
NmuCI GTSAC 2 cut(s) 352, 513
NspI RCATGY 1 cut(s) 947
PctI GAATGC 3 cut(s) 964, 1360, 1399
PdmI GAANNNNTTC 2 cut(s) 396, 1003
PfeI GAWTC 7 cut(s) 54, 412, 508, 635, 647, 806, 1229
PflFI GACNNNGTC 1 cut(s) 968
PkrI GCNGC 2 cut(s) 836, 976
PleI GAGTC 3 cut(s) 101, 359, 472
PpsI GAGTC 3 cut(s) 101, 359, 472
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 1232
PspEI GGTNACC 1 cut(s) 1420
PspFI CCCAGC 1 cut(s) 1395
PspGI CCWGG 1 cut(s) 1232
PspN4I GGNNCC 4 cut(s) 119, 365, 901, 912
PsuI RGATCY 2 cut(s) 363, 580
PsyI GACNNNGTC 1 cut(s) 968
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 3 cut(s) 254, 396, 912
RsaNI GTAC 3 cut(s) 253, 395, 911
RseI CAYNNNNRTG 3 cut(s) 128, 842, 1404
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 4 cut(s) 113, 684, 705, 849
SatI GCNGC 2 cut(s) 835, 975
Sau3AI GATC 5 cut(s) 61, 363, 406, 580, 677
ScaI AGTACT 1 cut(s) 396
SchI GAGTC 3 cut(s) 101, 360, 472
ScrFI CCNGG 1 cut(s) 1234
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 4 cut(s) 181, 462, 681, 780
SmiMI CAYNNNNRTG 3 cut(s) 128, 842, 1404
SmlI CTYRAG 2 cut(s) 934, 1286
SmoI CTYRAG 2 cut(s) 934, 1286
SsiI CCGC 1 cut(s) 974
SspMI CTAG 3 cut(s) 30, 810, 1310
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 1232
TaaI ACNGT 4 cut(s) 266, 436, 1073, 1453
TaiI ACGT 1 cut(s) 646
TaqI TCGA 1 cut(s) 638
TatI WGTACW 1 cut(s) 394
TauI GCSGC 1 cut(s) 977
TfiI GAWTC 7 cut(s) 54, 412, 508, 635, 647, 806, 1229
Tru1I TTAA 4 cut(s) 113, 684, 705, 849
Tru9I TTAA 4 cut(s) 113, 684, 705, 849
TscAI CASTG 4 cut(s) 64, 381, 629, 1078
TseFI GTSAC 2 cut(s) 352, 513
TseI GCWGC 1 cut(s) 834
Tsp45I GTSAC 2 cut(s) 352, 513
TspDTI ATGAA 8 cut(s) 87, 647, 882, 917, 1158, 1206, 1242, 1338
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 4 cut(s) 64, 381, 629, 1078
Tth111I GACNNNGTC 1 cut(s) 968
XagI CCTNNNNNAGG 1 cut(s) 821
XapI RAATTY 5 cut(s) 75, 490, 652, 709, 727
XbaI TCTAGA 1 cut(s) 1309
XceI RCATGY 1 cut(s) 947
XcmI CCANNNNNNNNNTGG 2 cut(s) 746, 1240
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 2 cut(s) 396, 1003
XspI CTAG 3 cut(s) 30, 810, 1310
ZrmI AGTACT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.