Rw1G004580

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006172
Physical Location & Seq
Reverse (-)
8776501 .. 8777230
730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004580.1

Sequence Viewer

Length: 699 bp
ATGGATATAAACAAAGAAACTAAGGAGCATATGCTTCATCAGACTCCCTTTTACAATGGGGACAATGGCAACTGCTTGATTCTATCCTCCCAACTGGGCGGTTTTGCTCATGCCTTTGGTCTTGAGGCTGCAATCCATGTTCGGATGGTCTCGGGTCCTAAAGATCTCCGAACTTTCGTAATTCATCTGTCAGAGAATACTGCAAGCTTACTCCTTCCTTTTATATATTCTACAACAACATCAACTGATTTGGAGACCTTGCGGAAACACGACAAGATGTTGGACGCAATGCTAACTAATGAAGTGGGGTCTGCACTCATGAGAGTGGCTGCAGCAGTGTATTCAGAAATACCGTCTCTTAAATTTATGAGGGAAACTCCTTTATGTAATGGAGTCGTTTCTTTTCACCGTGCTCCTATGTATGGGCTTATATGTGGATTGCTTGGGTTGGATAGCAGTACTTCCCAAAGAGCTTTCATGTTTATCACAATGAGAGATGTTATTTCTGCTGCAACAAGGCTGAATTTGGTCGGATCCCTTGGTGCAGCTGTTCTGCAACATCATATTGCTCCTATCTCTGACGCTATACTGAATCAATGGAAGGACATTCCAGTTGAGGAAGCATGCCAAACTGTTCCTTTGCTTGATATCGTGCAGGGTTGTCATAGCTACCTGTTTTCTAGATCGTTCTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0051336 GO:0051345 GO:0065007 GO:0065009 GO:1905181 GO:1905182
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UreF

Protein Analysis

232

Amino Acids

25.5

Weight (kDa)

6.18

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UreF PF01730 50 - 189 1.2e-13 UreF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 99, 262
AclWI GGATC 2 cut(s) 528, 541
AcsI RAATTY 2 cut(s) 362, 523
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 1 cut(s) 422
AluBI AGCT 4 cut(s) 207, 473, 548, 669
AluI AGCT 4 cut(s) 207, 473, 548, 669
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 3 cut(s) 154, 248, 360
AlwI GGATC 2 cut(s) 528, 541
Ama87I CYCGRG 1 cut(s) 151
ApeKI GCWGC 5 cut(s) 128, 329, 332, 509, 545
ApoI RAATTY 2 cut(s) 362, 523
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 1 cut(s) 155
BamHI GGATCC 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 5 cut(s) 115, 316, 344, 496, 557
BccI CCATC 1 cut(s) 139
BcoDI GTCTC 3 cut(s) 154, 248, 360
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 330
BglII AGATCT 1 cut(s) 163
BisI GCNGC 5 cut(s) 129, 330, 333, 510, 546
BlsI GCNGC 5 cut(s) 130, 331, 334, 511, 547
BmcAI AGTACT 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 155
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 2 cut(s) 156, 535
BmrI ACTGGG 1 cut(s) 104
BmuI ACTGGG 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 361, 393
BpuEI CTTGAG 1 cut(s) 143
BsaI GGTCTC 2 cut(s) 154, 248
BsaJI CCNNGG 1 cut(s) 538
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 99, 611
Bse3DI GCAATG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 538
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 422
BseMI GCAATG 1 cut(s) 294
BseNI ACTGG 2 cut(s) 99, 611
BseXI GCAGC 5 cut(s) 115, 316, 344, 496, 557
BsgI GTGCAG 3 cut(s) 297, 564, 674
BsiHKAI GWGCWC 1 cut(s) 415
BsiHKCI CYCGRG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 422
BsmAI GTCTC 3 cut(s) 154, 248, 360
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 74
Bso31I GGTCTC 2 cut(s) 154, 248
BsoBI CYCGRG 1 cut(s) 151
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 3 cut(s) 163, 533, 683
BspACI CCGC 2 cut(s) 99, 262
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 156, 535
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 2 cut(s) 528, 541
BspTNI GGTCTC 2 cut(s) 154, 248
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 2 cut(s) 99, 611
BssECI CCNNGG 1 cut(s) 538
BssMI GATC 3 cut(s) 163, 533, 683
BssT1I CCWWGG 1 cut(s) 538
Bst4CI ACNGT 3 cut(s) 354, 410, 634
BstC8I GCNNGC 2 cut(s) 205, 625
BstDEI CTNAG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 3 cut(s) 166, 536, 686
BstMAI GTCTC 3 cut(s) 154, 248, 360
BstMBI GATC 3 cut(s) 163, 533, 683
BstNSI RCATGY 1 cut(s) 627
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 5 cut(s) 115, 316, 344, 496, 557
BstX2I RGATCY 2 cut(s) 163, 533
BstYI RGATCY 2 cut(s) 163, 533
BtsCI GGATG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 2 cut(s) 205, 625
CciI TCATGA 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 155
CseI GACGC 2 cut(s) 293, 590
Csp6I GTAC 1 cut(s) 459
CviAII CATG 5 cut(s) 110, 137, 319, 478, 624
CviJI RGCY 8 cut(s) 128, 207, 329, 427, 473, 520, 548, 669
CviKI_1 RGCY 8 cut(s) 128, 207, 329, 427, 473, 520, 548, 669
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 1 cut(s) 21
DpnI GATC 3 cut(s) 165, 535, 685
DpnII GATC 3 cut(s) 163, 533, 683
Eco130I CCWWGG 1 cut(s) 538
Eco31I GGTCTC 2 cut(s) 154, 248
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 155
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 538
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 5 cut(s) 113, 140, 322, 481, 627
FaqI GGGAC 1 cut(s) 74
FatI CATG 5 cut(s) 109, 136, 318, 477, 623
FauNDI CATATG 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 129, 330, 333, 510, 546
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 5 cut(s) 129, 330, 333, 510, 546
FspBI CTAG 1 cut(s) 681
GluI GCNGC 5 cut(s) 129, 330, 333, 510, 546
HgaI GACGC 2 cut(s) 293, 590
Hin1II CATG 5 cut(s) 113, 140, 322, 481, 627
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 4 cut(s) 43, 79, 393, 592
HphI GGTGA 1 cut(s) 398
Hpy188I TCNGA 7 cut(s) 42, 144, 170, 193, 346, 533, 580
Hpy188III TCNNGA 4 cut(s) 122, 319, 681, 696
HpyAV CCTTC 2 cut(s) 224, 595
HpyCH4III ACNGT 3 cut(s) 354, 410, 634
HpyCH4V TGCA 8 cut(s) 131, 203, 314, 332, 512, 545, 556, 655
HpyF3I CTNAG 1 cut(s) 21
Hsp92II CATG 5 cut(s) 113, 140, 322, 481, 627
Kzo9I GATC 3 cut(s) 163, 533, 683
LmnI GCTCC 3 cut(s) 25, 418, 574
LpnPI CCDG 4 cut(s) 80, 624, 641, 686
Lsp1109I GCAGC 5 cut(s) 115, 316, 344, 496, 557
MaeI CTAG 1 cut(s) 681
MalI GATC 3 cut(s) 165, 535, 685
MboI GATC 3 cut(s) 163, 533, 683
MflI RGATCY 2 cut(s) 163, 533
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 3 cut(s) 180, 362, 523
MlyI GAGTC 2 cut(s) 37, 402
MmeI TCCRAC 3 cut(s) 261, 429, 511
MnlI CCTC 4 cut(s) 97, 118, 363, 610
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 548
NdeI CATATG 1 cut(s) 30
NdeII GATC 3 cut(s) 163, 533, 683
NlaIII CATG 5 cut(s) 113, 140, 322, 481, 627
NlaIV GGNNCC 2 cut(s) 156, 535
NspI RCATGY 1 cut(s) 627
PaeI GCATGC 1 cut(s) 627
PagI TCATGA 1 cut(s) 318
PfeI GAWTC 2 cut(s) 79, 592
PkrI GCNGC 5 cut(s) 130, 331, 334, 511, 547
PleI GAGTC 2 cut(s) 37, 401
PpsI GAGTC 2 cut(s) 37, 401
PpuMI RGGWCCY 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 155
PspN4I GGNNCC 2 cut(s) 156, 535
PspPI GGNCC 1 cut(s) 155
PspPPI RGGWCCY 1 cut(s) 155
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 2 cut(s) 163, 533
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 5 cut(s) 129, 330, 333, 510, 546
Sau3AI GATC 3 cut(s) 163, 533, 683
Sau96I GGNCC 1 cut(s) 155
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 2 cut(s) 37, 402
SduI GDGCHC 1 cut(s) 415
SetI ASST 6 cut(s) 209, 260, 475, 550, 671, 675
SfcI CTRYAG 1 cut(s) 330
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SphI GCATGC 1 cut(s) 627
Sse9I AATT 3 cut(s) 180, 362, 523
SsiI CCGC 2 cut(s) 99, 262
SspMI CTAG 1 cut(s) 681
StyI CCWWGG 1 cut(s) 538
TaaI ACNGT 3 cut(s) 354, 410, 634
TasI AATT 3 cut(s) 180, 362, 523
TatI WGTACW 1 cut(s) 458
TfiI GAWTC 2 cut(s) 79, 592
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 342
TseI GCWGC 5 cut(s) 128, 329, 332, 509, 545
TspDTI ATGAA 4 cut(s) 26, 173, 315, 466
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 155
XapI RAATTY 2 cut(s) 362, 523
XbaI TCTAGA 1 cut(s) 680
XceI RCATGY 1 cut(s) 627
XspI CTAG 1 cut(s) 681
ZrmI AGTACT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.