Rorug05G0497600

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006172
Physical Location & Seq
Forward (+)
67932534 .. 67941103
8570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0497600.1

Sequence Viewer

Length: 972 bp
ATGGCTAATGAGAAGGGTCTGATTGTGAGTTTCGGGGAGATGCTGATCGACTTTGTGCCAACTGTTTCGGGCGTTTCGCTGGCAGAAGCCCCGGCTTTCTTGAAAGCCCCCGGTGGCGCCCCCGCCAACGTGGCGATCGCGGTGGCTCGGCTCGGTGGCAACGCCTCCTTCGTCGGGAAGCTCGGCGCCGACGAGTTCGGGCACATGCTTGCTGGAATCTTGAAGGAGAACGGTGTCTCCGGACAGGGCATCCTGTTGGACCAAGGCGCACGTACCGCTCTGGCCTTCGTGACCCTACGCGCTGATGGAGAGCGCGAGTTCATGTTCTACCGGAACCCTAGCGCTGATATGCTGCTCAAGCCCGAAGAGCTCAATTTCGAGCTGATCAGATCCGCCAAAGTGTTCCACTATGGATCAATAAGCTTGATTGTGGAACCATGCAGGTCGGCACATCTGAAGGCAATGGAGGTCGCAAAAGATGCAGGTGCCTTGCTTTCCTACGACCCGAACCTCCGGCTACCACTGTGGCCCTCACCGGAGGAGGCACGCGAGCAGATAATGAGTATATGGGAAAAGGCTGAAGTGATCAAGGTCAGTGATGTGGAGCTTGAGTTTCTCACAGGAAATCCCAACATTGATGATGAATCTGCTATGAGTCTGTGGCACCCTAACTTGAAGCTCCTCCTTGTCACTCTTGGTGAAAAGGGTTGCAGATACTACACTAAGAATTTCCGTGGATGTGTGGAAGCTTTTCATGTAACTGCGGTAGATACTACTGGTGCTGGTGATTCATTTGTGGGTGCTCTGCTCGCAAAGATTGTTGATGATCAATCTGTTCTTGATGATGAGCAAAGGTTGAGGGAAGTACTCAAATTTGCAAATGCATGTGGAGCAATAACAACGACCAAGAAGGGAGCAATTCCTGCACTCCCCACTGAGTCTGAAGTCCTCGCCCTAATCAAAGGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0051336 GO:0051345 GO:0065007 GO:0065009 GO:1905181 GO:1905182
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UreF

Protein Analysis

323

Amino Acids

34.69

Weight (kDa)

5.06

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 7 - 312 2.1e-74 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 2 cut(s) 432, 473
AccB1I GGYRCC 4 cut(s) 116, 185, 485, 663
AccBSI CCGCTC 1 cut(s) 278
AccII CGCG 4 cut(s) 140, 300, 315, 549
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 5 cut(s) 123, 140, 276, 393, 764
AclWI GGATC 2 cut(s) 384, 421
AcsI RAATTY 2 cut(s) 727, 872
AcuI CTGAAG 3 cut(s) 476, 600, 963
AcyI GRCGYC 2 cut(s) 117, 186
AfaI GTAC 2 cut(s) 274, 867
AfeI AGCGCT 1 cut(s) 343
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 3 cut(s) 103, 223, 676
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
AluBI AGCT 7 cut(s) 181, 370, 382, 423, 607, 679, 749
AluI AGCT 7 cut(s) 181, 370, 382, 423, 607, 679, 749
Alw21I GWGCWC 2 cut(s) 372, 805
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 2 cut(s) 384, 421
Aor13HI TCCGGA 1 cut(s) 239
Aor51HI AGCGCT 1 cut(s) 343
AoxI GGCC 2 cut(s) 282, 527
ApeKI GCWGC 1 cut(s) 352
ApoI RAATTY 2 cut(s) 727, 872
AsiSI GCGATCGC 1 cut(s) 138
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 6 cut(s) 119, 188, 269, 302, 315, 344
AspS9I GGNCC 2 cut(s) 259, 528
AsuC2I CCSGG 2 cut(s) 92, 111
AsuHPI GGTGA 3 cut(s) 525, 710, 797
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 204
BanI GGYRCC 4 cut(s) 116, 185, 485, 663
BanII GRGCYC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 372, 805
BbvI GCAGC 1 cut(s) 339
BccI CCATC 1 cut(s) 299
BclI TGATCA 3 cut(s) 384, 585, 826
BcnI CCSGG 2 cut(s) 92, 111
BcoDI GTCTC 1 cut(s) 241
BfaI CTAG 1 cut(s) 339
BfoI RGCGCY 3 cut(s) 120, 189, 345
BfuAI ACCTGC 2 cut(s) 432, 473
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 1 cut(s) 353
BlsI GCNGC 1 cut(s) 354
BmcAI AGTACT 1 cut(s) 867
Bme1390I CCNGG 2 cut(s) 92, 111
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 259, 528
BmiI GGNNCC 6 cut(s) 118, 187, 335, 435, 487, 665
BmrFI CCNGG 2 cut(s) 92, 111
BmsI GCATC 3 cut(s) 30, 258, 469
BpuEI CTTGAG 2 cut(s) 341, 629
BpuMI CCSGG 2 cut(s) 92, 111
BsaAI YACGTR 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 44
BsaHI GRCGYC 2 cut(s) 117, 186
BsaJI CCNNGG 4 cut(s) 90, 109, 262, 733
BsaWI WCCGGW 3 cut(s) 239, 330, 535
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 596, 626
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 1 cut(s) 781
Bse3DI GCAATG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 44
BseAI TCCGGA 1 cut(s) 239
BseDI CCNNGG 4 cut(s) 90, 109, 262, 733
BseGI GGATG 2 cut(s) 249, 743
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 927
BseNI ACTGG 1 cut(s) 781
BseRI GAGGAG 2 cut(s) 554, 671
BseSI GKGCMC 1 cut(s) 204
BseXI GCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 4 cut(s) 140, 300, 315, 549
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 284, 529
BshNI GGYRCC 4 cut(s) 116, 185, 485, 663
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 372, 805
BsiSI CCGG 6 cut(s) 92, 111, 240, 331, 514, 536
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 241
BsnI GGCC 2 cut(s) 284, 529
Bsp1286I GDGCHC 3 cut(s) 204, 372, 805
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
BspACI CCGC 5 cut(s) 123, 140, 276, 393, 764
BspANI GGCC 2 cut(s) 284, 529
BspCNI CTCAG 1 cut(s) 928
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 4 cut(s) 140, 300, 315, 549
BspLI GGNNCC 6 cut(s) 118, 187, 335, 435, 487, 665
BspMI ACCTGC 2 cut(s) 432, 473
BspPI GGATC 2 cut(s) 384, 421
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 4 cut(s) 116, 185, 485, 663
BsrBI CCGCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 1 cut(s) 781
BssECI CCNNGG 4 cut(s) 90, 109, 262, 733
BssMI GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
BssNI GRCGYC 2 cut(s) 117, 186
BssT1I CCWWGG 1 cut(s) 262
Bst4CI ACNGT 3 cut(s) 64, 233, 525
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 2 cut(s) 117, 186
BstAPI GCANNNNNTGC 2 cut(s) 479, 923
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 5 cut(s) 81, 210, 547, 551, 810
BstDEI CTNAG 2 cut(s) 723, 936
BstDSI CCRYGG 1 cut(s) 733
BstF5I GGATG 2 cut(s) 249, 743
BstFNI CGCG 4 cut(s) 140, 300, 315, 549
BstH2I RGCGCY 3 cut(s) 120, 189, 345
BstHHI GCGC 6 cut(s) 119, 188, 269, 302, 315, 344
BstKTI GATC 7 cut(s) 48, 138, 387, 392, 416, 588, 829
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 8 cut(s) 131, 275, 358, 367, 479, 809, 890, 923
BstNSI RCATGY 2 cut(s) 208, 888
BstSCI CCNGG 2 cut(s) 90, 109
BstSLI GKGCMC 1 cut(s) 204
BstUI CGCG 4 cut(s) 140, 300, 315, 549
BstV1I GCAGC 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 2 cut(s) 284, 529
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 2 cut(s) 249, 743
BtsIMutI CAGTG 3 cut(s) 521, 601, 933
BveI ACCTGC 2 cut(s) 432, 473
Cac8I GCNNGC 5 cut(s) 81, 210, 547, 551, 810
CfoI GCGC 6 cut(s) 119, 188, 269, 302, 315, 344
Cfr13I GGNCC 2 cut(s) 259, 528
Csp6I GTAC 2 cut(s) 273, 866
CviAII CATG 5 cut(s) 205, 322, 438, 755, 885
CviQI GTAC 2 cut(s) 273, 866
DdeI CTNAG 2 cut(s) 723, 936
DinI GGCGCC 2 cut(s) 118, 187
DpnI GATC 7 cut(s) 47, 137, 386, 391, 415, 587, 828
DpnII GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 3 cut(s) 476, 600, 963
EcoICRI GAGCTC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 886
EcoT38I GRGCYC 1 cut(s) 372
EgeI GGCGCC 2 cut(s) 118, 187
EheI GGCGCC 2 cut(s) 118, 187
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 5 cut(s) 208, 325, 441, 758, 888
FatI CATG 5 cut(s) 204, 321, 437, 754, 884
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 3 cut(s) 384, 585, 826
Fnu4HI GCNGC 1 cut(s) 353
FokI GGATG 2 cut(s) 236, 750
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 1 cut(s) 353
FspBI CTAG 1 cut(s) 339
GlaI GCGC 6 cut(s) 118, 187, 268, 301, 314, 343
GluI GCNGC 1 cut(s) 353
HaeII RGCGCY 3 cut(s) 120, 189, 345
HaeIII GGCC 2 cut(s) 284, 529
HapII CCGG 6 cut(s) 92, 111, 240, 331, 514, 536
HhaI GCGC 6 cut(s) 119, 188, 269, 302, 315, 344
Hin1I GRCGYC 2 cut(s) 117, 186
Hin1II CATG 5 cut(s) 208, 325, 441, 758, 888
Hin6I GCGC 6 cut(s) 117, 186, 267, 300, 313, 342
HinP1I GCGC 6 cut(s) 117, 186, 267, 300, 313, 342
HindIII AAGCTT 2 cut(s) 421, 747
HinfI GANTC 5 cut(s) 216, 644, 655, 788, 938
HpaII CCGG 6 cut(s) 92, 111, 240, 331, 514, 536
HphI GGTGA 3 cut(s) 525, 710, 797
Hpy188I TCNGA 4 cut(s) 21, 389, 456, 943
Hpy188III TCNNGA 6 cut(s) 100, 175, 220, 240, 289, 839
Hpy99I CGWCG 2 cut(s) 176, 194
HpyAV CCTTC 6 cut(s) 7, 178, 217, 295, 451, 904
HpyCH4III ACNGT 3 cut(s) 64, 233, 525
HpyCH4IV ACGT 2 cut(s) 129, 271
HpyCH4V TGCA 6 cut(s) 441, 482, 711, 878, 884, 926
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 275, 358, 367, 479, 809, 890, 923
HpyF3I CTNAG 2 cut(s) 723, 936
HpySE526I ACGT 2 cut(s) 129, 271
Hsp92I GRCGYC 2 cut(s) 117, 186
Hsp92II CATG 5 cut(s) 208, 325, 441, 758, 888
HspAI GCGC 6 cut(s) 117, 186, 267, 300, 313, 342
KasI GGCGCC 2 cut(s) 116, 185
Kpn2I TCCGGA 1 cut(s) 239
Ksp22I TGATCA 3 cut(s) 384, 585, 826
Kzo9I GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 4 cut(s) 604, 684, 890, 914
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 3 cut(s) 30, 258, 469
MaeI CTAG 1 cut(s) 339
MaeII ACGT 2 cut(s) 129, 271
MaeIII GTNAC 3 cut(s) 289, 688, 757
MalI GATC 7 cut(s) 47, 137, 386, 391, 415, 587, 828
MbiI CCGCTC 1 cut(s) 278
MboI GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
MboII GAAGA 1 cut(s) 377
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 3 cut(s) 204, 372, 805
MluCI AATT 4 cut(s) 373, 727, 872, 918
Mly113I GGCGCC 2 cut(s) 117, 186
MlyI GAGTC 2 cut(s) 664, 947
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 9 cut(s) 175, 460, 521, 532, 535, 541, 692, 852, 959
Mph1103I ATGCAT 1 cut(s) 886
MroI TCCGGA 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 750
MspI CCGG 6 cut(s) 92, 111, 240, 331, 514, 536
MspR9I CCNGG 2 cut(s) 92, 111
MvnI CGCG 4 cut(s) 140, 300, 315, 549
MwoI GCNNNNNNNGC 8 cut(s) 131, 275, 358, 367, 479, 809, 890, 923
NarI GGCGCC 2 cut(s) 117, 186
NciI CCSGG 2 cut(s) 92, 111
NdeII GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
NlaIII CATG 5 cut(s) 208, 325, 441, 758, 888
NlaIV GGNNCC 6 cut(s) 118, 187, 335, 435, 487, 665
NmeAIII GCCGAG 2 cut(s) 127, 162
NmuCI GTSAC 2 cut(s) 289, 688
NsiI ATGCAT 1 cut(s) 886
NspI RCATGY 2 cut(s) 208, 888
PaqCI CACCTGC 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 360
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 189
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 3 cut(s) 216, 644, 788
PkrI GCNGC 1 cut(s) 354
Ple19I CGATCG 1 cut(s) 138
PleI GAGTC 2 cut(s) 663, 946
PluTI GGCGCC 2 cut(s) 120, 189
PpsI GAGTC 2 cut(s) 663, 946
Ppu21I YACGTR 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 372
PspN4I GGNNCC 6 cut(s) 118, 187, 335, 435, 487, 665
PspPI GGNCC 2 cut(s) 259, 528
PsuI RGATCY 1 cut(s) 389
PvuI CGATCG 1 cut(s) 138
RgaI GCGATCGC 1 cut(s) 138
RsaI GTAC 2 cut(s) 274, 867
RsaNI GTAC 2 cut(s) 273, 866
SacI GAGCTC 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 360
SatI GCNGC 1 cut(s) 353
Sau3AI GATC 7 cut(s) 45, 135, 384, 389, 413, 585, 826
Sau96I GGNCC 2 cut(s) 259, 528
ScaI AGTACT 1 cut(s) 867
SchI GAGTC 2 cut(s) 664, 947
ScrFI CCNGG 2 cut(s) 92, 111
SduI GDGCHC 3 cut(s) 204, 372, 805
SfaAI GCGATCGC 1 cut(s) 138
SfaNI GCATC 3 cut(s) 30, 258, 469
SfoI GGCGCC 2 cut(s) 118, 187
SgfI GCGATCGC 1 cut(s) 138
SinI GGWCC 1 cut(s) 259
SmlI CTYRAG 2 cut(s) 356, 608
SmoI CTYRAG 2 cut(s) 356, 608
Sse9I AATT 4 cut(s) 373, 727, 872, 918
SsiI CCGC 5 cut(s) 123, 140, 276, 393, 764
SspDI GGCGCC 2 cut(s) 116, 185
SspMI CTAG 1 cut(s) 339
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 90, 109
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 3 cut(s) 64, 233, 525
TaiI ACGT 2 cut(s) 132, 274
TaqI TCGA 2 cut(s) 48, 378
TasI AATT 4 cut(s) 373, 727, 872, 918
TatI WGTACW 1 cut(s) 865
TfiI GAWTC 3 cut(s) 216, 644, 788
TscAI CASTG 3 cut(s) 528, 601, 940
TseFI GTSAC 2 cut(s) 289, 688
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 2 cut(s) 289, 688
TspDTI ATGAA 4 cut(s) 310, 657, 743, 780
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 3 cut(s) 528, 601, 940
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 2 cut(s) 727, 872
XceI RCATGY 2 cut(s) 208, 888
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 1 cut(s) 339
ZrmI AGTACT 1 cut(s) 867
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.