Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003998

Physical Location & Seq

Genomic Coordinates Forward (+)

4738453 .. 4743030

Gene Structure 4578 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1036100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGCCATCAAGACACTCGGTCTAGTTAAAGGTTGTCTTCCAGAGGAATTAAAGAAGGTACTTTTATCTGTAGCTTCTGAATGGGGGGATGTAGTGGATGATTTGAATGCCTTGCAGGTCACCCCATTGAAAGGGGCAATGACTAATGAGGTCTATCAAATAAGTTGGCCTACGAGGAACAATGGTGACCTTTTCCGGAAAGTTCTGGTTCGGGTTTACGGTGAGGGAGTTGAAGTGTTTTTTAATCGGGAGAATGAAATTCAGACGTTCGAGTGCATGTCCAAGCACGGCCAGGGGCCGCGTCTTCTTGGGCGATTTGCAGAGGGACGGGTTGAGGAGTTCATTCATGCTAGAACGCTATCAGCTGCTGACCTCCGTGATCCCGAAATATCTGCTATTATAGCAGCTAAGCTGAGAGAGTTCCACAATTTGGATATGACTGGTCCGAGAAATGTAGTCCTTTGGGATAGAATGAGGAACTGGCTCAATGAGGCAAAAAACTTGTGTTCGATCAAAGACATACAGGAATTCGGCTTGGATACTTTGGAAGAGGAAATTAGTATGTTAGAGAAAGAACTGTCACAGGACTATCAAGAAATTGGGTTCTGTCACAATGACCTGCAGTACGGTAACATAATGATGGATGAAGAGACCAGATTAATCACTTTAATTGATTATGAATATGCCAGTTACAATCCTGTCGCTTATGACCTTGCAAATCATTTCTGTGAAATGGTAGCAAACTACCACTCTGAGACACCTCACGTTTTGGACTACAGGAATTATCCAGGTCTGGAGGAGCGCCAGAGATTTGTCCGTGCATATCTGAGCTCTGCAGGTTGCGAACCCAGTGATGCTGAGGTGGAGCAGCTAGTTGAAATAGCAGAGAAGTACACTTTGGCCAACCATCTCTTTTGGGGCTTATGGGGAATAATTTCGGGTCATGTGAACAAAATTGATTTTGACTACACGGAGTATGCGAGGCAGAGGTTTCAGCAGTACTGGTTGAGAAAGCCGGCATTGTTGGGTTCTTCAGGTGACTCCCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004103 GO:0004305 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006644 GO:0006646 GO:0006650 GO:0006656 GO:0006657 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009611 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046337 GO:0046470 GO:0046474 GO:0046486 GO:0050896 GO:0071704 GO:0090407 GO:0097164 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00564 ko01100 ko05231 map00564 map01100 map05231

Pfam Domains

Protein Families

Choline_kin_N Choline_kinase

Protein Analysis

350

Amino Acids

40.23

Weight (kDa)

4.9

Isoelectric Point (pI)

36.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Choline_kinase	PF01633	40 - 335	1.2e-97	Choline/ethanolamine kinase
APH	PF01636	41 - 245	1.3e-14	Phosphotransferase enzyme family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71697 AT4G09760 AT4G09760 AT4G09760 AT4G09760
fragaria_vesca	FvH4_2g12980 FvH4_2g12980 FvH4_2g12980 FvH4_2g12980
malus_domestica	MD05G1034200.v1.1 MD10G1036100.v1.1
prunus_persica	Prupe.8G041800_v2.0.a1 Prupe.8G041800_v2.0.a1 Prupe.8G041800_v2.0.a1 Prupe.8G041800_v2.0.a1
pyrus_communis	pycom05g02470 pycom10g02450
rosa_chinensis	RchiOBHm_Chr6g0274111
rosa_laevigata	RLG00000013577
rosa_multiflora	Rmu_ssc0000413.1_g000002
rosa_roxburghii	Rroxscaffold_7G00194640
rosa_rugosa	Rorug06G0078500 Rorug06G0078500 Rorug06G0078600 Rorug06G0078700 Rorug06G0078700 Rorug06G0078700 Rorug06G0078800
rosa_samantha	Rh6AG194600 Rh6BG198700 Rh6CG198500
rosa_wichuraiana	Rw0G007920 Rw6G016960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	106, 627, 827
AccB7I	CCANNNNNTGG	1 cut(s)	430
AccII	CGCG	1 cut(s)	301
AccIII	TCCGGA	1 cut(s)	195
AciI	CCGC	1 cut(s)	299
AclWI	GGATC	1 cut(s)	374
AcoI	YGGCCR	3 cut(s)	3, 289, 900
AcsI	RAATTY	2 cut(s)	258, 527
AcuI	CTGAAG	1 cut(s)	1017
AfaI	GTAC	4 cut(s)	60, 626, 893, 1001
AfiI	CCNNNNNNNGG	2 cut(s)	131, 430
AgsI	TTSAA	4 cut(s)	106, 130, 233, 878
AhdI	GACNNNNNGTC	1 cut(s)	18
AjnI	CCWGG	2 cut(s)	291, 787
AjuI	GAANNNNNNNTTGG	2 cut(s)	881, 913
AluBI	AGCT	6 cut(s)	74, 365, 407, 412, 831, 871
AluI	AGCT	6 cut(s)	74, 365, 407, 412, 831, 871
Alw21I	GWGCWC	1 cut(s)	833
Alw26I	GTCTC	2 cut(s)	644, 749
AlwI	GGATC	1 cut(s)	374
AlwNI	CAGNNNCTG	1 cut(s)	368
Aor13HI	TCCGGA	1 cut(s)	195
AoxI	GGCC	5 cut(s)	3, 167, 289, 296, 900
ApeKI	GCWGC	3 cut(s)	365, 404, 868
ApoI	RAATTY	2 cut(s)	258, 527
AseI	ATTAAT	1 cut(s)	659
Asp700I	GAANNNNTTC	1 cut(s)	934
AspLEI	GCGC	1 cut(s)	804
AspS9I	GGNCC	2 cut(s)	296, 443
AsuHPI	GGTGA	4 cut(s)	112, 197, 233, 1049
AvaII	GGWCC	1 cut(s)	443
BalI	TGGCCA	2 cut(s)	5, 902
BanII	GRGCYC	1 cut(s)	833
BbsI	GAAGAC	2 cut(s)	29, 296
Bbv12I	GWGCWC	1 cut(s)	833
BbvCI	CCTCAGC	1 cut(s)	858
BbvI	GCAGC	3 cut(s)	352, 416, 880
BccI	CCATC	3 cut(s)	14, 634, 915
BceAI	ACGGC	1 cut(s)	304
BcgI	CGANNNNNNTGC	2 cut(s)	374, 408
BciT130I	CCWGG	2 cut(s)	293, 789
BciVI	GTATCC	1 cut(s)	532
BcoDI	GTCTC	2 cut(s)	644, 749
BfaI	CTAG	3 cut(s)	23, 351, 872
BfmI	CTRYAG	4 cut(s)	69, 620, 775, 834
BfoI	RGCGCY	1 cut(s)	805
BfuAI	ACCTGC	3 cut(s)	106, 627, 827
BfuI	GTATCC	1 cut(s)	532
BisI	GCNGC	4 cut(s)	299, 366, 405, 869
BlpI	GCTNAGC	1 cut(s)	408
BlsI	GCNGC	4 cut(s)	300, 367, 406, 870
BmcAI	AGTACT	1 cut(s)	1001
Bme1390I	CCNGG	2 cut(s)	293, 789
Bme18I	GGWCC	1 cut(s)	443
BmeRI	GACNNNNNGTC	1 cut(s)	18
BmgT120I	GGNCC	2 cut(s)	296, 443
BmiI	GGNNCC	1 cut(s)	297
BmrFI	CCNGG	2 cut(s)	293, 789
BmrI	ACTGGG	1 cut(s)	843
BmsI	GCATC	1 cut(s)	844
BmuI	ACTGGG	1 cut(s)	843
BpiI	GAAGAC	2 cut(s)	29, 296
BpmI	CTGGAG	1 cut(s)	815
Bpu10I	CCTNAGC	1 cut(s)	858
Bpu1102I	GCTNAGC	1 cut(s)	408
BsaI	GGTCTC	1 cut(s)	644
BsaJI	CCNNGG	1 cut(s)	292
BsaWI	WCCGGW	1 cut(s)	195
BsaXI	ACNNNNNCTCC	2 cut(s)	219, 249
Bsc4I	CCNNNNNNNGG	2 cut(s)	131, 430
Bse118I	RCCGGY	1 cut(s)	1015
Bse1I	ACTGG	5 cut(s)	445, 485, 687, 849, 1007
Bse3DI	GCAATG	1 cut(s)	144
BseAI	TCCGGA	1 cut(s)	195
BseBI	CCWGG	2 cut(s)	293, 789
BseDI	CCNNGG	1 cut(s)	292
BseGI	GGATG	3 cut(s)	94, 103, 649
BseLI	CCNNNNNNNGG	2 cut(s)	131, 430
BseMI	GCAATG	1 cut(s)	144
BseMII	CTCAG	4 cut(s)	404, 744, 818, 849
BseNI	ACTGG	5 cut(s)	445, 485, 687, 849, 1007
BseRI	GAGGAG	2 cut(s)	350, 812
BseXI	GCAGC	3 cut(s)	352, 416, 880
Bsh1236I	CGCG	1 cut(s)	301
BshFI	GGCC	5 cut(s)	5, 169, 291, 298, 902
BsiHKAI	GWGCWC	1 cut(s)	833
BsiSI	CCGG	2 cut(s)	196, 1016
BslFI	GGGAC	1 cut(s)	339
BslI	CCNNNNNNNGG	2 cut(s)	131, 430
BsmAI	GTCTC	2 cut(s)	644, 749
BsmFI	GGGAC	1 cut(s)	339
BsmI	GAATGC	1 cut(s)	112
BsnI	GGCC	5 cut(s)	5, 169, 291, 298, 902
Bso31I	GGTCTC	1 cut(s)	644
Bsp1286I	GDGCHC	1 cut(s)	833
Bsp13I	TCCGGA	1 cut(s)	195
Bsp143I	GATC	2 cut(s)	379, 510
Bsp1720I	GCTNAGC	1 cut(s)	408
BspACI	CCGC	1 cut(s)	299
BspANI	GGCC	5 cut(s)	5, 169, 291, 298, 902
BspCNI	CTCAG	4 cut(s)	405, 745, 819, 850
BspEI	TCCGGA	1 cut(s)	195
BspFNI	CGCG	1 cut(s)	301
BspLI	GGNNCC	1 cut(s)	297
BspMAI	CTGCAG	2 cut(s)	624, 838
BspMI	ACCTGC	3 cut(s)	106, 627, 827
BspPI	GGATC	1 cut(s)	374
BspTNI	GGTCTC	1 cut(s)	644
BsrDI	GCAATG	1 cut(s)	144
BsrFI	RCCGGY	1 cut(s)	1015
BsrI	ACTGG	5 cut(s)	445, 485, 687, 849, 1007
BssAI	RCCGGY	1 cut(s)	1015
BssECI	CCNNGG	1 cut(s)	292
BssMI	GATC	2 cut(s)	379, 510
Bst2UI	CCWGG	2 cut(s)	293, 789
Bst4CI	ACNGT	3 cut(s)	221, 579, 629
Bst6I	CTCTTC	2 cut(s)	543, 642
BstC8I	GCNNGC	1 cut(s)	1017
BstDEI	CTNAG	5 cut(s)	408, 413, 753, 827, 858
BstEII	GGTNACC	2 cut(s)	118, 185
BstF5I	GGATG	3 cut(s)	94, 103, 649
BstFNI	CGCG	1 cut(s)	301
BstH2I	RGCGCY	1 cut(s)	805
BstHHI	GCGC	1 cut(s)	804
BstKTI	GATC	2 cut(s)	382, 513
BstMAI	GTCTC	2 cut(s)	644, 749
BstMBI	GATC	2 cut(s)	379, 510
BstMWI	GCNNNNNNNGC	1 cut(s)	401
BstNI	CCWGG	2 cut(s)	293, 789
BstNSI	RCATGY	1 cut(s)	280
BstPI	GGTNACC	2 cut(s)	118, 185
BstSCI	CCNGG	2 cut(s)	291, 787
BstSFI	CTRYAG	4 cut(s)	69, 620, 775, 834
BstUI	CGCG	1 cut(s)	301
BstV1I	GCAGC	3 cut(s)	352, 416, 880
BstV2I	GAAGAC	2 cut(s)	29, 296
BsuI	GTATCC	1 cut(s)	532
BsuRI	GGCC	5 cut(s)	5, 169, 291, 298, 902
BtsCI	GGATG	3 cut(s)	94, 103, 649
BtsIMutI	CAGTG	1 cut(s)	856
BveI	ACCTGC	3 cut(s)	106, 627, 827
Cac8I	GCNNGC	1 cut(s)	1017
CaiI	CAGNNNCTG	1 cut(s)	368
CfoI	GCGC	1 cut(s)	804
Cfr10I	RCCGGY	1 cut(s)	1015
Cfr13I	GGNCC	2 cut(s)	296, 443
CseI	GACGC	1 cut(s)	290
Csp6I	GTAC	4 cut(s)	59, 625, 892, 1000
CviAII	CATG	3 cut(s)	277, 347, 944
CviQI	GTAC	4 cut(s)	59, 625, 892, 1000
DdeI	CTNAG	5 cut(s)	408, 413, 753, 827, 858
DpnI	GATC	2 cut(s)	381, 512
DpnII	GATC	2 cut(s)	379, 510
DriI	GACNNNNNGTC	1 cut(s)	18
EaeI	YGGCCR	3 cut(s)	3, 289, 900
Eam1104I	CTCTTC	2 cut(s)	543, 642
Eam1105I	GACNNNNNGTC	1 cut(s)	18
EarI	CTCTTC	2 cut(s)	543, 642
Ecl136II	GAGCTC	1 cut(s)	831
Eco24I	GRGCYC	1 cut(s)	833
Eco31I	GGTCTC	1 cut(s)	644
Eco47I	GGWCC	1 cut(s)	443
Eco53kI	GAGCTC	1 cut(s)	831
Eco57I	CTGAAG	1 cut(s)	1017
Eco91I	GGTNACC	2 cut(s)	118, 185
EcoICRI	GAGCTC	1 cut(s)	831
EcoO65I	GGTNACC	2 cut(s)	118, 185
EcoRI	GAATTC	1 cut(s)	527
EcoRII	CCWGG	2 cut(s)	291, 787
EcoT38I	GRGCYC	1 cut(s)	833
FaeI	CATG	3 cut(s)	280, 350, 947
FalI	AAGNNNNNCTT	2 cut(s)	21, 53
FaqI	GGGAC	1 cut(s)	339
FatI	CATG	3 cut(s)	276, 346, 943
Fnu4HI	GCNGC	4 cut(s)	299, 366, 405, 869
FokI	GGATG	3 cut(s)	101, 110, 656
FriOI	GRGCYC	1 cut(s)	833
Fsp4HI	GCNGC	4 cut(s)	299, 366, 405, 869
FspBI	CTAG	3 cut(s)	23, 351, 872
GlaI	GCGC	1 cut(s)	803
GluI	GCNGC	4 cut(s)	299, 366, 405, 869
GsuI	CTGGAG	1 cut(s)	815
HaeII	RGCGCY	1 cut(s)	805
HaeIII	GGCC	5 cut(s)	5, 169, 291, 298, 902
HapII	CCGG	2 cut(s)	196, 1016
HgaI	GACGC	1 cut(s)	290
HhaI	GCGC	1 cut(s)	804
Hin1II	CATG	3 cut(s)	280, 350, 947
Hin6I	GCGC	1 cut(s)	802
HinP1I	GCGC	1 cut(s)	802
HinfI	GANTC	1 cut(s)	1040
HpaII	CCGG	2 cut(s)	196, 1016
HphI	GGTGA	4 cut(s)	112, 197, 233, 1049
Hpy166II	GTNNAC	3 cut(s)	217, 894, 949
Hpy188I	TCNGA	5 cut(s)	79, 264, 447, 754, 828
Hpy188III	TCNNGA	7 cut(s)	10, 41, 196, 248, 383, 593, 794
Hpy8I	GTNNAC	3 cut(s)	217, 894, 949
HpyAV	CCTTC	1 cut(s)	49
HpyCH4III	ACNGT	3 cut(s)	221, 579, 629
HpyCH4IV	ACGT	2 cut(s)	266, 765
HpyCH4V	TGCA	7 cut(s)	115, 276, 320, 622, 716, 821, 836
HpyF10VI	GCNNNNNNNGC	1 cut(s)	401
HpyF3I	CTNAG	5 cut(s)	408, 413, 753, 827, 858
HpySE526I	ACGT	2 cut(s)	266, 765
Hsp92II	CATG	3 cut(s)	280, 350, 947
HspAI	GCGC	1 cut(s)	802
Kpn2I	TCCGGA	1 cut(s)	195
KroI	GCCGGC	1 cut(s)	1015
KroNI	GCCGGC	1 cut(s)	1017
Kzo9I	GATC	2 cut(s)	379, 510
LmnI	GCTCC	2 cut(s)	799, 865
Lsp1109I	GCAGC	3 cut(s)	352, 416, 880
LweI	GCATC	1 cut(s)	844
MaeI	CTAG	3 cut(s)	23, 351, 872
MaeII	ACGT	2 cut(s)	266, 765
MaeIII	GTNAC	7 cut(s)	118, 185, 579, 608, 629, 689, 1037
MalI	GATC	2 cut(s)	381, 512
MboI	GATC	2 cut(s)	379, 510
MboII	GAAGA	5 cut(s)	29, 296, 560, 659, 1023
MhlI	GDGCHC	1 cut(s)	833
MlsI	TGGCCA	2 cut(s)	5, 902
MluNI	TGGCCA	2 cut(s)	5, 902
MlyI	GAGTC	1 cut(s)	1034
Mox20I	TGGCCA	2 cut(s)	5, 902
MroI	TCCGGA	1 cut(s)	195
MroNI	GCCGGC	1 cut(s)	1015
MroXI	GAANNNNTTC	1 cut(s)	934
MscI	TGGCCA	2 cut(s)	5, 902
MseI	TTAA	5 cut(s)	27, 50, 243, 659, 668
MslI	CAYNNNNRTG	2 cut(s)	638, 726
Msp20I	TGGCCA	2 cut(s)	5, 902
MspA1I	CMGCKG	1 cut(s)	365
MspI	CCGG	2 cut(s)	196, 1016
MspR9I	CCNGG	2 cut(s)	293, 789
Mva1269I	GAATGC	1 cut(s)	112
MvaI	CCWGG	2 cut(s)	293, 789
MvnI	CGCG	1 cut(s)	301
MwoI	GCNNNNNNNGC	1 cut(s)	401
NaeI	GCCGGC	1 cut(s)	1017
NdeII	GATC	2 cut(s)	379, 510
NgoMIV	GCCGGC	1 cut(s)	1015
NlaIII	CATG	3 cut(s)	280, 350, 947
NlaIV	GGNNCC	1 cut(s)	297
NmuCI	GTSAC	5 cut(s)	118, 185, 579, 608, 1037
NspI	RCATGY	1 cut(s)	280
PcsI	WCGNNNNNNNCGW	1 cut(s)	977
PctI	GAATGC	1 cut(s)	112
PdiI	GCCGGC	1 cut(s)	1017
PdmI	GAANNNNTTC	1 cut(s)	934
PflMI	CCANNNNNTGG	1 cut(s)	430
PkrI	GCNGC	4 cut(s)	300, 367, 406, 870
PleI	GAGTC	1 cut(s)	1034
PpsI	GAGTC	1 cut(s)	1034
PshBI	ATTAAT	1 cut(s)	659
Psp124BI	GAGCTC	1 cut(s)	833
Psp6I	CCWGG	2 cut(s)	291, 787
PspEI	GGTNACC	2 cut(s)	118, 185
PspGI	CCWGG	2 cut(s)	291, 787
PspN4I	GGNNCC	1 cut(s)	297
PspPI	GGNCC	2 cut(s)	296, 443
PstI	CTGCAG	2 cut(s)	624, 838
PstNI	CAGNNNCTG	1 cut(s)	368
PvuII	CAGCTG	1 cut(s)	365
RsaI	GTAC	4 cut(s)	60, 626, 893, 1001
RsaNI	GTAC	4 cut(s)	59, 625, 892, 1000
RseI	CAYNNNNRTG	2 cut(s)	638, 726
SacI	GAGCTC	1 cut(s)	833
SaqAI	TTAA	5 cut(s)	27, 50, 243, 659, 668
SatI	GCNGC	4 cut(s)	299, 366, 405, 869
Sau3AI	GATC	2 cut(s)	379, 510
Sau96I	GGNCC	2 cut(s)	296, 443
ScaI	AGTACT	1 cut(s)	1001
SchI	GAGTC	1 cut(s)	1034
ScrFI	CCNGG	2 cut(s)	293, 789
SduI	GDGCHC	1 cut(s)	833
SfaNI	GCATC	1 cut(s)	844
SfcI	CTRYAG	4 cut(s)	69, 620, 775, 834
SinI	GGWCC	1 cut(s)	443
SmiMI	CAYNNNNRTG	2 cut(s)	638, 726
SsiI	CCGC	1 cut(s)	299
SspMI	CTAG	3 cut(s)	23, 351, 872
SstI	GAGCTC	1 cut(s)	833
StyD4I	CCNGG	2 cut(s)	291, 787
TaaI	ACNGT	3 cut(s)	221, 579, 629
TaiI	ACGT	2 cut(s)	269, 768
TaqI	TCGA	2 cut(s)	270, 509
TaqII	GACCGA	1 cut(s)	8
TatI	WGTACW	2 cut(s)	891, 999
TauI	GCSGC	1 cut(s)	301
Tru1I	TTAA	5 cut(s)	27, 50, 243, 659, 668
Tru9I	TTAA	5 cut(s)	27, 50, 243, 659, 668
TscAI	CASTG	1 cut(s)	856
TseFI	GTSAC	5 cut(s)	118, 185, 579, 608, 1037
TseI	GCWGC	3 cut(s)	365, 404, 868
Tsp45I	GTSAC	5 cut(s)	118, 185, 579, 608, 1037
TspDTI	ATGAA	5 cut(s)	270, 331, 335, 660, 693
TspGWI	ACGGA	3 cut(s)	365, 806, 986
TspRI	CASTG	1 cut(s)	856
Van91I	CCANNNNNTGG	1 cut(s)	430
VpaK11BI	GGWCC	1 cut(s)	443
VspI	ATTAAT	1 cut(s)	659
XapI	RAATTY	2 cut(s)	258, 527
XceI	RCATGY	1 cut(s)	280
XmnI	GAANNNNTTC	1 cut(s)	934
XspI	CTAG	3 cut(s)	23, 351, 872
ZrmI	AGTACT	1 cut(s)	1001

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.