MD10G1103100.v1.1

sulfhydryl oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009180
Physical Location & Seq
Forward (+)
16766402 .. 16768863
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1103100.v1.1.491

Sequence Viewer

Length: 612 bp
ATGTGTATGAAGATGTCGGAGCATCCATTCCACGCCCTCTTTCAAACTTTTGCAAAAGTCTCCAATTCGATTCAAACCCAACTCTCGAATCTCATCGCCCACTCTCACGAGCCATCACCCACCGCCCAAAAACGCCTCCTCTCTTCCTCCTCCAAAGTGGCAGGCTCATCAGACAATGGCTCCGCCCAATCCCAGCCTAAAGATATCCTCAGCACGAAAATGGGAAAATCGGTTTCTCCTTTGACTAAAGAAGAACTCGGAAGATCCACTTGGACGTTTCTTCACACTCTTGCTGCTCAGTATCCAGATAAACCGACAAGGCAACAGAAGAAGGATGTAAAGGAACTGATGTCGATATTATCACGGATGTACCCCTGCAAGGAATGTGCAGATCACTTTCAAGAAATTCTGAGATCAAATCCTGTACAGGCTGGATCGCATGCTGAGTTCTCCCAGTGGCTGTGTCATGTGCATAATACAGTTAATAGAAGCCTTAACAAACCGGTGTTCCCCTGTGAACGCGTTGATGCACGCTGGGGCAAGTTAGAGTGTGAACAGCGTGCTTGCGATCTACTAGGAATTACCGGAGATTTTGGCGACAATGCATTTTAA

Protein Analysis

204

Amino Acids

22.72

Weight (kDa)

8.68

Isoelectric Point (pI)

63.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Evr1_Alr PF04777 88 - 179 5.2e-35 Erv1 / Alr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 522
AciI CCGC 2 cut(s) 123, 183
AclWI GGATC 2 cut(s) 258, 442
AcsI RAATTY 1 cut(s) 405
AfaI GTAC 2 cut(s) 371, 426
AfiI CCNNNNNNNGG 1 cut(s) 379
AflIII ACRYGT 1 cut(s) 520
AgeI ACCGGT 1 cut(s) 502
AgsI TTSAA 3 cut(s) 44, 74, 401
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 2 cut(s) 258, 442
AlwNI CAGNNNCTG 1 cut(s) 460
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 405
AsiGI ACCGGT 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 108
BaeI ACNNNNGTAYC 2 cut(s) 353, 386
BauI CACGAG 1 cut(s) 107
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 280
BccI CCATC 1 cut(s) 121
BciVI GTATCC 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 575
BfuI GTATCC 1 cut(s) 312
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 181
BmrI ACTGGG 1 cut(s) 448
BmsI GCATC 2 cut(s) 31, 517
BmuI ACTGGG 1 cut(s) 448
Bpu10I CCTNAGC 1 cut(s) 209
BsaWI WCCGGW 2 cut(s) 502, 584
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 454
BseGI GGATG 3 cut(s) 22, 340, 372
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 4 cut(s) 223, 311, 401, 435
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 2 cut(s) 128, 139
BseXI GCAGC 1 cut(s) 280
BseYI CCCAGC 2 cut(s) 192, 534
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 522
BshTI ACCGGT 1 cut(s) 502
BsiSI CCGG 2 cut(s) 503, 585
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 64
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 263, 391, 413, 434, 568
BspACI CCGC 2 cut(s) 123, 183
BspCNI CTCAG 4 cut(s) 222, 310, 402, 436
BspFNI CGCG 1 cut(s) 522
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 2 cut(s) 258, 442
BsrFI RCCGGY 1 cut(s) 502
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 1 cut(s) 454
BssAI RCCGGY 1 cut(s) 502
BssMI GATC 5 cut(s) 263, 391, 413, 434, 568
BssSI CACGAG 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 1 cut(s) 148
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 5 cut(s) 163, 441, 532, 561, 565
BstDEI CTNAG 4 cut(s) 209, 297, 410, 444
BstF5I GGATG 3 cut(s) 22, 340, 372
BstFNI CGCG 1 cut(s) 522
BstKTI GATC 5 cut(s) 266, 394, 416, 437, 571
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 5 cut(s) 263, 391, 413, 434, 568
BstNSI RCATGY 1 cut(s) 443
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuI GTATCC 1 cut(s) 312
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 3 cut(s) 22, 340, 372
BtsIMutI CAGTG 1 cut(s) 461
Cac8I GCNNGC 5 cut(s) 163, 441, 532, 561, 565
CaiI CAGNNNCTG 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 502
Csp6I GTAC 2 cut(s) 370, 425
CspAI ACCGGT 1 cut(s) 502
CviAII CATG 2 cut(s) 440, 467
CviJI RGCY 7 cut(s) 112, 165, 180, 196, 431, 460, 492
CviKI_1 RGCY 7 cut(s) 112, 165, 180, 196, 431, 460, 492
CviQI GTAC 2 cut(s) 370, 425
DdeI CTNAG 4 cut(s) 209, 297, 410, 444
DpnI GATC 5 cut(s) 265, 393, 415, 436, 570
DpnII GATC 5 cut(s) 263, 391, 413, 434, 568
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
EciI GGCGGA 1 cut(s) 172
Eco32I GATATC 1 cut(s) 205
EcoRV GATATC 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 607
FaeI CATG 2 cut(s) 443, 470
FaiI YATR 4 cut(s) 8, 441, 468, 474
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FatI CATG 2 cut(s) 439, 466
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 3 cut(s) 9, 347, 379
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 1 cut(s) 575
GluI GCNGC 1 cut(s) 294
GsaI CCCAGC 2 cut(s) 196, 538
HapII CCGG 2 cut(s) 503, 585
Hin1II CATG 2 cut(s) 443, 470
HinfI GANTC 2 cut(s) 70, 88
HpaII CCGG 2 cut(s) 503, 585
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 518, 554
Hpy188I TCNGA 4 cut(s) 19, 172, 260, 411
Hpy188III TCNNGA 4 cut(s) 85, 107, 305, 401
Hpy8I GTNNAC 2 cut(s) 518, 554
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 6 cut(s) 53, 378, 389, 472, 530, 605
HpyF3I CTNAG 4 cut(s) 209, 297, 410, 444
HpySE526I ACGT 1 cut(s) 275
Hsp92II CATG 2 cut(s) 443, 470
Kzo9I GATC 5 cut(s) 263, 391, 413, 434, 568
LmnI GCTCC 2 cut(s) 19, 185
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 2 cut(s) 31, 517
MaeI CTAG 1 cut(s) 575
MaeII ACGT 1 cut(s) 275
MalI GATC 5 cut(s) 265, 393, 415, 436, 570
MboI GATC 5 cut(s) 263, 391, 413, 434, 568
MboII GAAGA 6 cut(s) 22, 135, 263, 272, 273, 340
MflI RGATCY 1 cut(s) 263
MluCI AATT 3 cut(s) 64, 405, 579
MluI ACGCGT 1 cut(s) 520
MnlI CCTC 6 cut(s) 47, 146, 149, 157, 160, 218
Mph1103I ATGCAT 1 cut(s) 607
MseI TTAA 3 cut(s) 483, 495, 610
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 2 cut(s) 503, 585
MvnI CGCG 1 cut(s) 522
NdeII GATC 5 cut(s) 263, 391, 413, 434, 568
NlaIII CATG 2 cut(s) 443, 470
NlaIV GGNNCC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 1 cut(s) 443
PaeI GCATGC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 70, 88
PinAI ACCGGT 1 cut(s) 502
PkrI GCNGC 1 cut(s) 295
PspFI CCCAGC 2 cut(s) 192, 534
PspN4I GGNNCC 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 2 cut(s) 371, 426
RsaNI GTAC 2 cut(s) 370, 425
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 3 cut(s) 483, 495, 610
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 5 cut(s) 263, 391, 413, 434, 568
SetI ASST 1 cut(s) 278
SfaNI GCATC 2 cut(s) 31, 517
SmiMI CAYNNNNRTG 1 cut(s) 218
SphI GCATGC 1 cut(s) 443
Sse9I AATT 3 cut(s) 64, 405, 579
SsiI CCGC 2 cut(s) 123, 183
SspMI CTAG 1 cut(s) 575
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 1 cut(s) 278
TaqI TCGA 3 cut(s) 68, 86, 353
TasI AATT 3 cut(s) 64, 405, 579
TatI WGTACW 1 cut(s) 424
TfiI GAWTC 2 cut(s) 70, 88
Tru1I TTAA 3 cut(s) 483, 495, 610
Tru9I TTAA 3 cut(s) 483, 495, 610
TscAI CASTG 1 cut(s) 461
TseI GCWGC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 1 cut(s) 461
XapI RAATTY 1 cut(s) 405
XceI RCATGY 1 cut(s) 443
XspI CTAG 1 cut(s) 575
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.