RchiOBHm_Chr6g0253051

sulfhydryl oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009180
Physical Location & Seq
Forward (+)
8255333 .. 8257616
2284 bp
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UTR
Exon/CDS
Intron
PRQ22691

Sequence Viewer

Length: 432 bp
ATGACCCCACCCATCTCCAACCCCAAGATCTCATCAACAACAAGAGAATTTTATGGCTCGGCTGGTCCTGTGACGAAAGAAGGGCTCGGAAGAGCCACTTGGACTTTTCTTCACACTCTTGCTGCTCAGTATCCGGATAATCCAACGAGGCAACAGAAGAAGGATGTAAAAGAATTGATGTCAATACTATCACGAATGTACCCCTGCAAGGAATGTGCAAATCATTTTCAAGAAATTCTGAGATCAAATCCTGTACAGGCTGGATCTCATGCTGAATTCTCCCAATGGCTATGTCATGTGCATAATACTGTCAACAGAAGCCTTAACAAAGTGGTTTTCCCCTGTGAACGAGTTGATGCAAGGTGGGGCAAGTTAGAGTGCGAACAGCGTGCTTGCGATGTACTAGGAAGTACCACAAGTTTTTGGAAATAA

Protein Analysis

143

Amino Acids

16.26

Weight (kDa)

8.99

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Evr1_Alr PF04777 31 - 122 4.5e-34 Erv1 / Alr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 133
AclWI GGATC 1 cut(s) 271
AcsI RAATTY 3 cut(s) 47, 234, 275
AfaI GTAC 4 cut(s) 200, 255, 402, 412
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 1 cut(s) 230
AjuI GAANNNNNNNTTGG 2 cut(s) 82, 114
AlwI GGATC 1 cut(s) 271
Aor13HI TCCGGA 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 3 cut(s) 47, 234, 275
AspS9I GGNCC 1 cut(s) 65
AvaII GGWCC 1 cut(s) 65
BaeI ACNNNNGTAYC 2 cut(s) 182, 215
BanII GRGCYC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 109
BccI CCATC 1 cut(s) 20
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciVI GTATCC 1 cut(s) 141
BfaI CTAG 1 cut(s) 404
BfuI GTATCC 1 cut(s) 141
BglII AGATCT 1 cut(s) 27
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 65
BmsI GCATC 1 cut(s) 346
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 208
BseAI TCCGGA 1 cut(s) 133
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 2 cut(s) 140, 230
BseXI GCAGC 1 cut(s) 109
BsiSI CCGG 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 253
Bsp143I GATC 3 cut(s) 27, 242, 263
BspCNI CTCAG 2 cut(s) 139, 231
BspEI TCCGGA 1 cut(s) 133
BspPI GGATC 1 cut(s) 271
BspQI GCTCTTC 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 253
BssMI GATC 3 cut(s) 27, 242, 263
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 253
BstC8I GCNNGC 2 cut(s) 390, 394
BstDEI CTNAG 2 cut(s) 126, 239
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 3 cut(s) 30, 245, 266
BstMBI GATC 3 cut(s) 27, 242, 263
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 2 cut(s) 27, 263
BstYI RGATCY 2 cut(s) 27, 263
BsuI GTATCC 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 390, 394
Cfr13I GGNCC 1 cut(s) 65
Csp6I GTAC 4 cut(s) 199, 254, 401, 411
CviAII CATG 2 cut(s) 269, 296
CviJI RGCY 7 cut(s) 57, 62, 85, 95, 260, 289, 321
CviKI_1 RGCY 7 cut(s) 57, 62, 85, 95, 260, 289, 321
CviQI GTAC 4 cut(s) 199, 254, 401, 411
DdeI CTNAG 2 cut(s) 126, 239
DpnI GATC 3 cut(s) 29, 244, 265
DpnII GATC 3 cut(s) 27, 242, 263
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 65
EcoRI GAATTC 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 87
FaeI CATG 2 cut(s) 272, 299
FaiI YATR 5 cut(s) 54, 270, 292, 297, 303
FalI AAGNNNNNCTT 2 cut(s) 82, 114
FatI CATG 2 cut(s) 268, 295
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 404
GluI GCNGC 1 cut(s) 123
HapII CCGG 1 cut(s) 134
Hin1II CATG 2 cut(s) 272, 299
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HpaII CCGG 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 313, 347
Hpy188I TCNGA 2 cut(s) 89, 240
Hpy188III TCNNGA 3 cut(s) 134, 192, 230
Hpy8I GTNNAC 2 cut(s) 313, 347
HpyAV CCTTC 2 cut(s) 74, 154
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4V TGCA 4 cut(s) 207, 218, 301, 359
HpyF3I CTNAG 2 cut(s) 126, 239
Hsp92II CATG 2 cut(s) 272, 299
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 27, 242, 263
LguI GCTCTTC 1 cut(s) 85
LpnPI CCDG 8 cut(s) 48, 81, 147, 217, 242, 246, 264, 355
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 70
MalI GATC 3 cut(s) 29, 244, 265
MboI GATC 3 cut(s) 27, 242, 263
MboII GAAGA 3 cut(s) 101, 102, 169
MflI RGATCY 2 cut(s) 27, 263
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 4 cut(s) 47, 173, 234, 275
MmeI TCCRAC 2 cut(s) 42, 167
MnlI CCTC 1 cut(s) 141
MroI TCCGGA 1 cut(s) 133
MseI TTAA 1 cut(s) 324
MspI CCGG 1 cut(s) 134
NdeII GATC 3 cut(s) 27, 242, 263
NlaIII CATG 2 cut(s) 272, 299
NmeAIII GCCGAG 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 70
PciSI GCTCTTC 1 cut(s) 85
PkrI GCNGC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 65
PsuI RGATCY 2 cut(s) 27, 263
RsaI GTAC 4 cut(s) 200, 255, 402, 412
RsaNI GTAC 4 cut(s) 199, 254, 401, 411
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 3 cut(s) 27, 242, 263
Sau96I GGNCC 1 cut(s) 65
SduI GDGCHC 1 cut(s) 87
SetI ASST 1 cut(s) 365
SfaNI GCATC 1 cut(s) 346
SinI GGWCC 1 cut(s) 65
Sse9I AATT 4 cut(s) 47, 173, 234, 275
SspMI CTAG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 310
TasI AATT 4 cut(s) 47, 173, 234, 275
TatI WGTACW 2 cut(s) 253, 400
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 65
XapI RAATTY 3 cut(s) 47, 234, 275
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.