MD10G1145200.v1.1

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008638
Physical Location & Seq
Reverse (-)
23158581 .. 23162621
4041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1145200.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGCCAAGCATTATCCTAATCCAACAGATTGGATGGCGTCAGGTTTTGCCAGATGAGAATGTATGCTGCTCTTCTGAACTTGACATGTTTCACTTGGGTTCTCCGCAACTGGGTCTCATCATTTTCCTGCCGGCTGGAGCCCTCAAACGGTCAAACCAGAAAAGTGCTGCTGATTTGAAGGGGATGGATGAAAAACTTGAATCCAAAGGCAAAGTTTGTGTTACGGGGGCTTCTGGCTATCTGGCATCTTGCCTTATAAAGCGGCTTCTCTTATCGGGCTATCATGTAATTGGAACCGTCAGAGATCTAGGAAACCAGAAAACGTTGGCACATTTATGGAAGCTGGAAGGAGCTAAAGAAAGACTCAGATTGGTGGAGGCTGATTTAATGAAAGAATGCAGCTTTGATGATGCAATCTTGGGATGCCATGGTGTCTTCCATTCTGCTTCCCCTGTATTGAAGAAACCTTCATCTGATCCAAAGAGAGAAGTCTTGGAGCCAGCGATTGAGGGTACCTTGAACGTGTTGCGTTCGTGTAAGAAAAATCCATCTCTGAGGCGAGTGGTTCTCACTTCATCTTCTACAGCTCTAAGGAATAGACCCGATGAAGATTTAGCATCCTGTGTAAAACTGGATGAATCATCTTGGAGCTCTGTGGAACACTGCCAGAGACTTCAGCTCTGGTACCCTTTATCTAAAGTTCTAGCCGAGAAGGCAGCGGGGGATTTTTGCAAAGAAAATGGGATCGATTTAGTAACTGTTCTGCCTGCGTGGGTGATTGGACCTAGTTTGTCACCTGATCTATGCTTGACTGCATCACGGGTACTCAGCCTGCTTAAAGGGGATACCGTATGGTATGGAAGAATGGGATATGTTCACATTGATGATGTTGCACTCTGTCACATCTTAGTTTATGAGCATGAAAGTGCTCATGGTCGATATCTTTGTAGCTCAACAGTTTTGGACAACGATGAGTTGGCTTCCTTATTATCCATGCGATATCCTTCCTTACCTATTGCCAAAAGGTTGAAGCAAGTAAGCAGACCATATTATGAGTTCGACACGTCAAAGTTGCAGAGTCTAGGATTCAAGTTCAAGAGTATCCAGGAGATGTTTGATGATTGCATTGACTCTTTGGTGGAGCAAGGCCATCTTTCTGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

389

Amino Acids

42.97

Weight (kDa)

6.82

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 69 - 149 8.1e-06 NmrA-like family
Epimerase PF01370 72 - 309 5.3e-20 NAD dependent epimerase/dehydratase family
NAD_binding_4 PF07993 74 - 263 2.2e-11 Male sterility protein
3Beta_HSD PF01073 74 - 261 3.2e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 74 - 195 8.2e-08 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 76 - 203 8.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc65I GGTACC 2 cut(s) 512, 684
AccB1I GGYRCC 2 cut(s) 512, 684
AciI CCGC 3 cut(s) 104, 262, 719
AclI AACGTT 1 cut(s) 323
AclWI GGATC 2 cut(s) 470, 752
AcuI CTGAAG 1 cut(s) 659
AcyI GRCGYC 1 cut(s) 37
AfaI GTAC 3 cut(s) 514, 686, 825
AfiI CCNNNNNNNGG 2 cut(s) 110, 147
AflIII ACRYGT 3 cut(s) 84, 522, 1062
AgsI TTSAA 7 cut(s) 178, 200, 460, 520, 1030, 1090, 1096
AjiI CACGTC 1 cut(s) 1065
AjnI CCWGG 1 cut(s) 1104
AluBI AGCT 7 cut(s) 343, 353, 402, 587, 651, 679, 951
AluI AGCT 7 cut(s) 343, 353, 402, 587, 651, 679, 951
Alw21I GWGCWC 2 cut(s) 653, 931
Alw26I GTCTC 2 cut(s) 119, 664
AlwI GGATC 2 cut(s) 470, 752
AoxI GGCC 1 cut(s) 1147
ApeKI GCWGC 4 cut(s) 66, 167, 399, 716
Asp718I GGTACC 2 cut(s) 512, 684
AspS9I GGNCC 1 cut(s) 782
AsuHPI GGTGA 2 cut(s) 786, 787
AvaII GGWCC 1 cut(s) 782
BanI GGYRCC 2 cut(s) 512, 684
BanII GRGCYC 2 cut(s) 142, 653
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 2 cut(s) 653, 931
BbvI GCAGC 4 cut(s) 53, 154, 411, 728
BccI CCATC 4 cut(s) 27, 178, 556, 1158
BciT130I CCWGG 1 cut(s) 1106
BciVI GTATCC 2 cut(s) 838, 1112
BcoDI GTCTC 2 cut(s) 119, 664
BfaI CTAG 5 cut(s) 308, 704, 786, 1082, 1165
BfmI CTRYAG 1 cut(s) 582
BfuI GTATCC 2 cut(s) 838, 1112
BglI GCCNNNNNGGC 1 cut(s) 713
BglII AGATCT 1 cut(s) 304
BisI GCNGC 5 cut(s) 67, 168, 263, 400, 717
BlsI GCNGC 5 cut(s) 68, 169, 264, 401, 718
Bme1390I CCNGG 1 cut(s) 1106
Bme18I GGWCC 1 cut(s) 782
BmgBI CACGTC 1 cut(s) 1065
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 5 cut(s) 139, 295, 498, 514, 686
BmrFI CCNGG 1 cut(s) 1106
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 5 cut(s) 254, 400, 413, 626, 824
BmuI ACTGGG 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 427
BplI GAGNNNNNCTC 2 cut(s) 552, 584
BpmI CTGGAG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 747
BsaHI GRCGYC 1 cut(s) 37
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 427
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 147
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 114, 636
BseBI CCWGG 1 cut(s) 1106
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 6 cut(s) 38, 189, 193, 428, 617, 640
BseLI CCNNNNNNNGG 2 cut(s) 110, 147
BseMII CTCAG 3 cut(s) 379, 545, 841
BseNI ACTGG 2 cut(s) 114, 636
BseXI GCAGC 4 cut(s) 53, 154, 411, 728
BsgI GTGCAG 1 cut(s) 1143
BshFI GGCC 1 cut(s) 1149
BshNI GGYRCC 2 cut(s) 512, 684
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 2 cut(s) 653, 931
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 110, 147
BsmAI GTCTC 2 cut(s) 119, 664
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 1 cut(s) 1149
Bso31I GGTCTC 1 cut(s) 119
Bsp1286I GDGCHC 3 cut(s) 142, 653, 931
Bsp143I GATC 4 cut(s) 304, 475, 744, 799
Bsp19I CCATGG 1 cut(s) 427
BspACI CCGC 3 cut(s) 104, 262, 719
BspANI GGCC 1 cut(s) 1149
BspCNI CTCAG 3 cut(s) 378, 546, 840
BspDI ATCGAT 1 cut(s) 747
BspLI GGNNCC 5 cut(s) 139, 295, 498, 514, 686
BspPI GGATC 2 cut(s) 470, 752
BspQI GCTCTTC 1 cut(s) 76
BspT107I GGYRCC 2 cut(s) 512, 684
BspTNI GGTCTC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 114, 636
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 4 cut(s) 304, 475, 744, 799
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 1106
Bst4CI ACNGT 5 cut(s) 150, 298, 760, 850, 958
Bst6I CTCTTC 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 37
BstC8I GCNNGC 4 cut(s) 132, 501, 768, 833
BstDEI CTNAG 5 cut(s) 365, 554, 590, 827, 907
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 6 cut(s) 38, 189, 193, 428, 617, 640
BstKTI GATC 4 cut(s) 307, 478, 747, 802
BstMAI GTCTC 2 cut(s) 119, 664
BstMBI GATC 4 cut(s) 304, 475, 744, 799
BstMWI GCNNNNNNNGC 1 cut(s) 713
BstNI CCWGG 1 cut(s) 1106
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 1104
BstSFI CTRYAG 1 cut(s) 582
BstV1I GCAGC 4 cut(s) 53, 154, 411, 728
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 304
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 1 cut(s) 304
Bsu15I ATCGAT 1 cut(s) 747
BsuI GTATCC 2 cut(s) 838, 1112
BsuRI GGCC 1 cut(s) 1149
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 1 cut(s) 427
BtrI CACGTC 1 cut(s) 1065
BtsCI GGATG 6 cut(s) 38, 189, 193, 428, 617, 640
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 4 cut(s) 132, 501, 768, 833
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 782
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 26
Csp6I GTAC 3 cut(s) 513, 685, 824
CviAII CATG 6 cut(s) 85, 284, 428, 920, 932, 994
CviQI GTAC 3 cut(s) 513, 685, 824
DdeI CTNAG 5 cut(s) 365, 554, 590, 827, 907
DpnI GATC 4 cut(s) 306, 477, 746, 801
DpnII GATC 4 cut(s) 304, 475, 744, 799
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 142, 653
Eco31I GGTCTC 1 cut(s) 119
Eco32I GATATC 2 cut(s) 941, 1001
Eco47I GGWCC 1 cut(s) 782
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 659
EcoICRI GAGCTC 1 cut(s) 651
EcoRII CCWGG 1 cut(s) 1104
EcoRV GATATC 2 cut(s) 941, 1001
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 142, 653
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 6 cut(s) 88, 287, 431, 923, 935, 997
FalI AAGNNNNNCTT 1 cut(s) 1137
FatI CATG 6 cut(s) 84, 283, 427, 919, 931, 993
FauI CCCGC 1 cut(s) 712
Fnu4HI GCNGC 5 cut(s) 67, 168, 263, 400, 717
FokI GGATG 6 cut(s) 45, 196, 200, 435, 604, 647
FriOI GRGCYC 2 cut(s) 142, 653
Fsp4HI GCNGC 5 cut(s) 67, 168, 263, 400, 717
FspBI CTAG 5 cut(s) 308, 704, 786, 1082, 1165
GluI GCNGC 5 cut(s) 67, 168, 263, 400, 717
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 1 cut(s) 1149
HapII CCGG 1 cut(s) 131
HgaI GACGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 6 cut(s) 88, 287, 431, 923, 935, 997
HinfI GANTC 6 cut(s) 200, 363, 638, 1078, 1086, 1130
HpaII CCGG 1 cut(s) 131
HphI GGTGA 2 cut(s) 786, 787
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 5 cut(s) 76, 302, 368, 475, 555
Hpy188III TCNNGA 1 cut(s) 1096
Hpy8I GTNNAC 1 cut(s) 877
HpyAV CCTTC 5 cut(s) 172, 341, 477, 706, 1014
HpyCH4III ACNGT 5 cut(s) 150, 298, 760, 850, 958
HpyCH4IV ACGT 3 cut(s) 323, 522, 1064
HpyCH4V TGCA 8 cut(s) 399, 413, 732, 815, 893, 1075, 1125, 1160
HpyF10VI GCNNNNNNNGC 1 cut(s) 713
HpyF3I CTNAG 5 cut(s) 365, 554, 590, 827, 907
HpySE526I ACGT 3 cut(s) 323, 522, 1064
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 6 cut(s) 88, 287, 431, 923, 935, 997
KpnI GGTACC 2 cut(s) 516, 688
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 4 cut(s) 304, 475, 744, 799
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 5 cut(s) 137, 350, 496, 648, 1141
Lsp1109I GCAGC 4 cut(s) 53, 154, 411, 728
LweI GCATC 5 cut(s) 254, 400, 413, 626, 824
MaeI CTAG 5 cut(s) 308, 704, 786, 1082, 1165
MaeII ACGT 3 cut(s) 323, 522, 1064
MaeIII GTNAC 4 cut(s) 220, 754, 792, 899
MalI GATC 4 cut(s) 306, 477, 746, 801
MboI GATC 4 cut(s) 304, 475, 744, 799
MboII GAAGA 6 cut(s) 63, 427, 472, 570, 620, 873
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 3 cut(s) 142, 653, 931
MluCI AATT 1 cut(s) 288
MlyI GAGTC 3 cut(s) 357, 1087, 1124
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 4 cut(s) 152, 370, 502, 549
MroNI GCCGGC 1 cut(s) 130
MseI TTAA 2 cut(s) 386, 837
MslI CAYNNNNRTG 3 cut(s) 334, 882, 924
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 1106
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 1 cut(s) 1106
MwoI GCNNNNNNNGC 1 cut(s) 713
NaeI GCCGGC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 427
NdeII GATC 4 cut(s) 304, 475, 744, 799
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 6 cut(s) 88, 287, 431, 923, 935, 997
NlaIV GGNNCC 5 cut(s) 139, 295, 498, 514, 686
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 2 cut(s) 792, 899
NspI RCATGY 1 cut(s) 88
PciI ACATGT 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 76
PctI GAATGC 1 cut(s) 401
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 3 cut(s) 200, 638, 1086
PfoI TCCNGGA 1 cut(s) 1104
PkrI GCNGC 5 cut(s) 68, 169, 264, 401, 718
PleI GAGTC 3 cut(s) 357, 1086, 1124
PpsI GAGTC 3 cut(s) 357, 1086, 1124
PscI ACATGT 1 cut(s) 84
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 653
Psp1406I AACGTT 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 1104
PspGI CCWGG 1 cut(s) 1104
PspN4I GGNNCC 5 cut(s) 139, 295, 498, 514, 686
PspPI GGNCC 1 cut(s) 782
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 3 cut(s) 514, 686, 825
RsaNI GTAC 3 cut(s) 513, 685, 824
RseI CAYNNNNRTG 3 cut(s) 334, 882, 924
SacI GAGCTC 1 cut(s) 653
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 2 cut(s) 386, 837
SatI GCNGC 5 cut(s) 67, 168, 263, 400, 717
Sau3AI GATC 4 cut(s) 304, 475, 744, 799
Sau96I GGNCC 1 cut(s) 782
SchI GAGTC 3 cut(s) 357, 1087, 1124
ScrFI CCNGG 1 cut(s) 1106
SduI GDGCHC 3 cut(s) 142, 653, 931
SfaNI GCATC 5 cut(s) 254, 400, 413, 626, 824
SfcI CTRYAG 1 cut(s) 582
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 3 cut(s) 334, 882, 924
Sse9I AATT 1 cut(s) 288
SsiI CCGC 3 cut(s) 104, 262, 719
SspMI CTAG 5 cut(s) 308, 704, 786, 1082, 1165
SstI GAGCTC 1 cut(s) 653
StyD4I CCNGG 1 cut(s) 1104
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 5 cut(s) 150, 298, 760, 850, 958
TaiI ACGT 3 cut(s) 326, 525, 1067
TaqI TCGA 3 cut(s) 747, 937, 1059
TasI AATT 1 cut(s) 288
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 3 cut(s) 200, 638, 1086
Tru1I TTAA 2 cut(s) 386, 837
Tru9I TTAA 2 cut(s) 386, 837
TscAI CASTG 1 cut(s) 668
TseFI GTSAC 2 cut(s) 792, 899
TseI GCWGC 4 cut(s) 66, 167, 399, 716
Tsp45I GTSAC 2 cut(s) 792, 899
TspDTI ATGAA 7 cut(s) 204, 404, 459, 564, 621, 651, 936
TspRI CASTG 1 cut(s) 668
VpaK11BI GGWCC 1 cut(s) 782
XceI RCATGY 1 cut(s) 88
XspI CTAG 5 cut(s) 308, 704, 786, 1082, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.